explorer.bio-rad.com Captivating Science Education 454 Classroom Kits 455 Laboratory Equipment and Consumables 458 Biotechnology Explorer™ Educational Products Classroom Kits explorer.bio-rad.com Captivating Science Education Our goal is to excite and inspire educators about the cutting-edge science of biotechnology and enable them to take that enthusiasm back to the classroom. We want to help teachers spend less time prepping and more time teaching. The centerpiece of the Bio-Rad education program comprises the Biotechnology Explorer™ kits — labs “in a box” — which provide the comprehensive applications that teachers need to update their syllabi and keep their content current. Supported by a complete line of research-quality equipment and supplies, the objective of the Biotechnology Explorer program is to support and revitalize life science education by providing educators with tools to keep up in the science-learning race. Biotechnology Explorer kit applications align with current life science education standards and performance indicators and meet the most rigorous college preparatory requirements. As a leading supplier to the life science research, clinical diagnostics, and food testing industries, Bio-Rad has a closeup view of where research and innovation are going. This enables us to develop products that are at the forefront of new technologies. Today’s students must be prepared to live and compete in a global economy driven by science and technology. From basics to best practices, Bio-Rad is committed to promoting scientific literacy by partnering with teachers, schools, and local educational organizations to inspire a new generation of scientists. 454 | Biotechnology Explorer Educational Products www.bio-rad.com Classroom Kits explorer.bio-rad.com Classroom Kits Biotechnology Explorer™ Kits A Foundation for Science Literacy Biotechnology Explorer kits address the critical need for inquiry-based activity — an important component of scientific literacy for an educated citizenry and a launch point of experience and practical training for students interested in careers in biotechnology. Bio-Rad offers a variety of kits that build upon key concepts in core life science curriculum content. Introductory kits include the Genes in a Bottle™ and pGLO™ bacterial transformation kits, advanced techniques are covered in kits such as the Crime Scene Investigator PCR Basics™ and GMO Investigator™ kits, and the Cloning and Sequencing Explorer Series guides students through an entire molecular biology workflow. See Ordering Information for the complete list of classroom kits. Environmental and Health Science Evolution Scientific Inquiry Life Science Core Content Cell and Molecuar Biology Chemistry of Life Genetics For more information, request the current Biotechnology Explorer catalog (bulletin 2112). Ordering Information Catalog # Description 166-5035EDU 166-5030EDU 166-2300EDU 166-0003EDU 166-0013EDU 166-0005EDU 166-0006EDU 166-0008EDU 166-2900EDU 166-2400EDU 166-2700EDU 166-2800EDU 166-0007EDU 166-0001EDU 166-0002EDU 166-2600EDU 166-2660EDU 166-2100EDU 166-2500EDU 166-2560EDU 166-5000EDU Biofuel Enzyme Kit, provides materials for 32 students or 8 workstations Microbes and Health Kit, provides materials for 32 students or 8 workstations Genes in a Bottle Kit, includes 1 DNA extraction module (166-2000EDU) and 2 DNA necklace modules (166-2200EDU); provides materials for 36 students or 9 workstations pGLO Bacterial Transformation Kit, provides materials for 32 students or 8 workstations pGLO Kit SDS-PAGE Extension, provides materials for 32 students or 8 workstations Green Fluorescent Protein (GFP) Chromatography Kit, provides materials for 32 students or 8 workstations Secrets of the Rainforest Kit, provides materials for 32 students or 8 workstations Size Exclusion Chromatography Kit, provides materials for 32 students or 8 workstations Got Protein? Kit, provides materials for 320 students or 80 workstations ELISA Immuno Explorer Kit, provides materials for 48 students or 12 workstations Comparative Proteomics Kit I: Protein Profiler Module, provides materials for 32 students or 8 workstations Comparative Proteomics Kit II: Western Blot Module, provides materials for 32 students or 8 workstations Forensic DNA Fingerprinting Kit, provides materials for 32 students or 8 workstations Analysis of Precut Lambda DNA Kit, provides materials for 32 students or 8 workstations Restriction Digestion and Analysis of Lambda DNA Kit, provides materials for 32 students or 8 workstations Crime Scene Investigator PCR Basics Kit, provides materials for 32 students or 8 workstations Crime Scene Investigator PCR Basics Real-Time PCR Starter Kit, provides materials for 32 students or 8 workstations plus additional real-time reagents for further studies PV92 PCR Informatics Kit, provides materials for 32 students or 8 workstations GMO Investigator Kit, provides materials for 32 students or 8 workstations GMO Investigator Real-Time PCR Starter Kit, provides materials for 32 students or 8 workstations plus additional real-time reagents for further studies Cloning and Sequencing Explorer Series, provides materials for 12 workstations Price, U.S. $ Inquire 95.00 134.00 89.00 85.00 101.00 146.00 75.00 160.00 124.00 163.00 187.00 130.00 89.00 101.00 160.00 520.00 199.00 175.00 528.00 1,495.00 EDU price discounts are for qualified educational institutions and educators only. Items are available at list price for noneducators (must be ordered without an EDU suffix). www.bio-rad.com Biotechnology Explorer Educational Products | 455 Classroom Kits explorer.bio-rad.com New Biofuel Enzyme Kit Reveal the power of enzyme kinetics by illustrating the theory through a real-world application to biofuels. Help your students learn how an enzyme influences the rate of a reaction. Discover how enzymes work and what makes them so powerful. Through guided inquiry activities, your students will determine how temperature, pH, the concentration of a substrate, and the concentration of an enzyme affect an enzymatic reaction. Biofuels are commonly used to power vehicles, heat homes, and provide fuel for cooking. New technologies provide the ability to convert pollution into renewable biofuel. Discover why enzymes are the basis for these advances. Can enzymes solve global warming? Let your students decide. Benefits of this kit include: ■■ ■■ Aligns with AP Biology Lab 2 Contains no caustic reagents Enables both qualitative and quantitative measurement of reactions ■■ Guides instruction on enzyme kinetics and biofuel energy sources ■■ ■■ Complete in one 45 minute lab session Ordering Information Catalog # Description Price, U.S. $ 166-5035EDU Biofuel Enzyme Kit, provides materials for 32 students or 8 workstations Inquire Cloning and Sequencing Explorer Series Equip students with technical skills for entry-level lab positions, or inspire them to pursue graduate degrees in scientific research. The Cloning and Sequencing Explorer Series has been designed with the assistance of undergraduate and community college educators to meet the needs of biotechnology and biology course instructors who want students to understand how molecular biology skills and techniques are applied to real-world research projects. This lab course provides students with the opportunity to perform novel research, allowing them to clone and sequence a gene from an uncharacterized plant species and to add to the body of scientific knowledge around the world. The series provides a fully developed and ready-togo lab course, including relevant background, protocols that work, and student assessment. From DNA extraction to computer-based sequence analysis, this modular kit is designed as a 6–8 week series of lab activities in which students clone and analyze GAPDH, a plant housekeeping gene that encodes glyceraldehyde-3-phosphate dehydrogenase. Microbial Culturing GAPDH PCR PCR Purification Ligation and Transformation Plasmid Purification DNA Sequencing Plasmid Purification Module • Plasmid isolation Nucleic Acid Extraction 456 | Biotechnology Explorer Educational Products Electrophoresis Bioinformatics www.bio-rad.com Classroom Kits explorer.bio-rad.com With the Cloning and Sequencing Explorer Series, students will: ■■ ■■ ■■ Extract plant source genomic DNA — use degenerate primers to perform nested PCR to amplify a major portion of the GAPC gene, a housekeeping gene in the GAPDH family that is essential for respiration, the most basic of biological processes Clone the gene fragment — transform the bacteria with the fragment, screen clones using plasmid minipreps and restriction enzymes, and send positive clones to be sequenced Perform extensive bioinformatic analysis on their novel sequences — conduct BLAST searches, assemble forward and reverse sequences into contigs, identify introns and exons, and transcribe and translate gene sequences into proteins Upon completion of the lab course, students may elect to submit novel sequences to the National Institutes of Health National Center for Biotechnology Information (NCBI) databases, thereby making their research available to the worldwide scientific community. This laboratory course is designed to ensure success for you and your students. Tailored controls permit the continuation of the experiment to its conclusion. This allows students to make mistakes, learn from them, and still be exposed to every step of the workflow within a specific time period. Students will experience firsthand the satisfaction that comes from a completed and successful research project, which may encourage them to pursue a career in research. Ordering Information Catalog # Description Price, U.S. $ 166-5000EDU*Complete Cloning and Sequencing Explorer Series, includes all 8 modules plus Curriculum Resource CD, 12 workstations 166-5005EDU Nucleic Acid Extraction Module 166-5010EDU GAPDH PCR Module 166-0451EDU Electrophoresis Module 732-6300EDU PCR Kleen Spin Purification Module 166-5015EDU Ligation and Transformation Module 166-5020EDU Microbial Culturing Module 732-6400EDU Aurum Plasmid Mini Purification Module 166-5025EDU Sequencing and Bioinformatics Module 166-5001EDU Curriculum Resource CD 166-5002EDU Cloning and Sequencing Explorer Series Instruction Manual, printed in full color 1,495.00 165.00 387.00 58.00 57.00 302.00 95.00 152.00 250.00 29.00 190.00 * Ships with both temperature sensitive and room temperature components. Immediately store temperature sensitive items at 4°C or –20°C as indicated. EDU price discounts are for qualified educational institutions and educators only. Items are available at list price for noneducators (must be ordered without an EDU suffix). www.bio-rad.com Biotechnology Explorer Educational Products | 457 Laboratory Equipment and Consumables explorer.bio-rad.com Laboratory Equipment and Consumables New Plastic Consumables Bio-Rad plastic racks and storage units provide the ultimate in ease of storage and sample organization. The green rack can hold up to eighty 1.5 or 2.0 ml tubes; the storage boxes can hold up to one hundred 1.5 or 2.0 ml tubes. Storage Boxes Green Rack Ordering Information See Also Pipet tips: pages 446–449. Catalog # Description 166-0481 166-0482 Green Rack, set of 5 Storage Boxes, set of 5, multicolored Price, U.S. $ 12.86 64.29 Micropipets These adjustable-volume digital pipets deliver exceptional comfort, durability, and performance, and are guaranteed to function efficiently and reliably for years. Micropipets are available in professional or classroom grade. Features of both grades include: ■■ ■■ ■■ Adjustable digital dial Tip ejector Accommodation of standard pipet tips Each grade is available in four models to cover all applications requiring transfer of small volumes of precious solutions: 0.5–10 µl, 2–20 µl, 20–200 µl, and 100–1,000 µl. Professional Adjustable-Volume Micropipets Classroom Digital Micropipets Ordering Information Catalog # Description 166-0505 166-0506 166-0507 166-0508 166-0550 166-0551 166-0552 166-0553 Professional Micropipet, adjustable volume, 0.5–10 µl Professional Micropipet, adjustable volume, 2–20 µl Professional Micropipet, adjustable volume, 20–200 µl Professional Micropipet, adjustable volume, 100–1,000 µl Classroom Micropipet, adjustable volume, 0.5–10 µl Classroom Micropipet, adjustable volume, 2–20 µl Classroom Micropipet, adjustable volume, 20–200 µl Classroom Micropipet, adjustable volume, 100–1,000 µl 458 | Biotechnology Explorer Educational Products Price, U.S. $ 294.67 294.67 294.67 294.67 148.00 148.00 148.00 148.00 www.bio-rad.com Laboratory Equipment and Consumables explorer.bio-rad.com See Also Laboratory Equipment Microtubes: pages 450–451. Centrifuges Mini centrifuge — includes microtube and PCR strip tube rotors and 0.4 and 0.5 ml tube adaptors; the maximum speed is 6,000 rpm (2,000 x g) PCR tubes: pages 433–434. ■■ ■■ ■■ Model 16K microcentrifuge — accommodates 1.5 or 2.0 ml tubes and has a quick-spin feature; the maximum speed is 14,000 rpm (16,000 x g). Safe for coldroom operation PCR tube adaptor for model 16K microcentrifuge — holds two PCR 8-tube strips or 16 individual 0.2 ml tubes UV Illuminators Long-wave UV lamp and UV penlight — designed and optimized for use during green fluorescent protein– based activities including with our pGLO™ bacterial transformation, pGLO kit SDS-PAGE extension, GFP chromatography, and Secrets of the Rainforest™ kits Model 16K Microcentrifuge (PCR tube adaptor sold separately) ■■ PowerPac™ Mini Power Supply ■■ ■■ ■■ 100 V or 200 V constant voltage output 200 mA maximum current 40 W maximum power Mixing Devices NEW Mini rocker — provides superior threedimensional mixing action; gentle yet thorough rocking makes it ideal to use for western blot incubations and gel staining. Compact footprint requires minimal benchspace Mini Centrifuge (includes PCR tube adaptor) Long-wave UV Lamp Penlight ■■ ■■ ■■ ■■ PowerPac Mini Power Supply NEW Tube roller — allows mixing of samples both horizontally and vertically; its compact design enables it to fit inside our mini incubation oven, making it ideal for mixing liquid cultures while incubating. Compact footprint requires minimal benchspace Tube Roller R-2000 vortexer — a general-purpose vortex mixer; B a flathead dimpled adaptor is also available ltraRocker ™ rocking platform — dual-platform U rocker that can be used for staining and destaining gels and blots, Southern hybridization, and overnight incubation Mini Rocker BR-2000 Vortexer (flathead dimpled adaptor sold separately) UltraRocker Rocking Platform www.bio-rad.com Biotechnology Explorer Educational Products | 459 Laboratory Equipment and Consumables explorer.bio-rad.com Temperature Control Equipment NEW Digital dry bath — digitally controlled dry bath that is ideal for a multitude of laboratory procedures where incubation of samples is needed; it’s accurate, built for safe continuous operation, economical, and versatile ■■ ■■ ■■ ■■ NEW DyNA Chill cooler — keeps precious samples (up to 12 in 1.5–2.0 ml microtubes) chilled; simply cool the DyNA Chill cooler to the desired temperature overnight, then you’re ready to chill samples without the mess of an ice bucket. Samples can also be easily transported ini incubation oven — can accommodate up to eighty M 6.5 cm plates and operates at temperatures up to 60˚C DyNA Chill Digital Dry Bath ater bath — temperature controlled, dependable, W affordable water bath that includes stainless steel tank and lid, electrostatically applied finish that resists rust, corrosion, and scratches, over-temperature protection, and thermometer Mini Incubation Oven Water Bath Ordering Information Catalog # Description Centrifuges 166-0603 166-0613 166-0623 166-0602 166-0612 166-0620 Mini Centrifuge, 120 V Mini Centrifuge, 220 V Mini Centrifuge, 220 V (UK) Model 16K Microcentrifuge, 120 V Model 16K Microcentrifuge, 220 V PCR Tube Adaptor, for model 16K microcentrifuge UV Illuminators 166-0500 Long-wave UV Lamp, requires 4 AA batteries 166-0530 Long-wave UV Penlight Power Supply 165-5048 PowerPac Mini Power Supply, 120/220 V Mixing Devices 166-0710 Mini Rocker, 120 V, includes blotting box, power cord 166-0720 Mini Rocker, 230 V (EU/UK), includes blotting box, 2 power cords (EU/UK) 166-0711 Tube Roller, 120 V, includes 2 tube carousels for 1.5 and 15 ml tubes 166-0721 Tube Roller, 230 V, includes 2 tube carousels for 1.5 and 15 ml tubes 166-0722 Tube Roller, 230 V (UK), includes 2 tube carousels for 1.5 and 15 ml tubes 166-0610 BR-2000 Vortexer, 120 V 166-0611 BR-2000 Vortexer, 220 V 166-0621 BR-2000 Vortexer, 220 V (UK) 166-0622 Flathead Dimpled Adaptor, for BR-2000 vortexer 166-0709 UltraRocker Rocking Platform, 120 V 166-0719 UltraRocker Rocking Platform, 220 V Temperature Control Equipment 166-0562 Digital Dry Bath, 120 V, includes 1.5 ml heating block, power cord 166-0563 Digital Dry Bath, 230 V (EU/UK), includes 1.5 ml heating block, 2 power cords (EU/UK) 166-0564 DyNA Chill Cooler, maintains sample temperature between –15°C and ambient for up to 8 hr without ice 166-0501 Mini Incubation Oven, 120 V 166-0521 Mini Incubation Oven, 220 V 166-0504 Temperature Controlled Water Bath, 120 V 166-0524 Temperature Controlled Water Bath, 220 V 460 | Biotechnology Explorer Educational Products Price, U.S. $ 427.14 Inquire Inquire 2,714.29 Inquire 140.00 40.00 12.50 Inquire 561.43 Inquire 664.29 Inquire Inquire 392.86 Inquire Inquire 64.29 1,040.00 Inquire 611.43 Inquire 94.29 524.29 Inquire 842.86 Inquire www.bio-rad.com