Pill Bug Habitat Preferences Designing Your Own Experiment We need to collect and bring in Pill Bugs and Sow Bugs!!! We will use them in experiments that we design ourselves next week! While most people call them pill bugs, they are also called doodle bugs and rolly pollies, which has various spellings. Sow bugs are very similar in appearance, but they do not roll up into a ball when disturbed. Where can you find Pill Bugs and Sow Bugs? Isopods breathe with gills, so pill bugs and sow bugs, although they live on land, they are restricted to areas with high humidity, under rocks or logs, in leaf litter or in crevices. They prefer shady areas, and some species are nocturnal. Therefore, the recommended way to search for some would be after dark, in areas that during the day are shady, under some logs in some very moist soil. To attract them, water soil or leaf litter in the shade and cover with plastic, piece of plywood or cardboard. Keep the area moist and check under the covering in a couple days. How to collect – have fun! Before looking for isopods, prepare a container and tools to gather the isopods. To collect them, use a spoon or shovel and a container. Look under a rock or log and be prepared to collect the isopods quickly before they scurry away from the light. Gently scoop up soil with the isopods and place them in the container. Look on the underside of the log or stone for others. They can be gently picked or brushed off with a finger into the container. Pill bugs often curl up and can be picked up individually or scooped up with the spoon. If you are going to keep the isopods a couple days before placing them in the classroom, use a plastic margarine or cottage cheese container with small holes poked in the lid and a moistened piece of paper towel lightly crumple inside. Use an old pie tin to sort the isopods from the soil before placing them in the container. The paper towel must be kept moist or they will die. A slice of potato or apple will provide the pill bugs with both food and moisture. Pre-Lab Activity Answer each of the following questions. 1. What is the difference between qualitative and quantitative data? 2. What is an independent variable? 3. What is a dependent variable? 4. What is the difference between a control and an experimental group? 5. Describe the environmental conditions in the places where you found your pill bugs. 1. What is the difference between qualitative and quantitative data? 2. What is an independent variable? 3. What is a dependent variable? 4. What is the difference between a control and an experimental group? 5. Describe the environmental conditions in the places where you found your pill bugs. Objectives • To gain experience and understanding about the importance of experimental design • To gain experience in forming and revising hypotheses • To demonstrate the use of a control and replicates/trials in an experiment • To be able to identify the independent and dependent variables in an experiment • To understand how to gather and record scientific data, both qualitative and quantitative • To learn about pill bug behavior Instructions Step one: Initial observations Observe the pill bugs for 5 minutes and take note of their behavior. Try introducing them to some new situations, and see how they react! Begin generating some ideas for a hypothesis to test. Note some of your initial pill bug observations in your Data section. Instructions continued Step two: Design your experiment Based on your initial observations recorded above, decide what you would like to test, and determine what your variables are. Your experiment should help to determine pill bug habitat preferences. Consider where you found your pill bugs as well as your initial observations when choosing your independent variable. 1. 2. 3. 4. 5. 6. Name the independent variable of your experiment (the one that you will manipulate). Name the dependent variable of your experiment (the one that you will measure). Now, state your hypothesis in terms of the effect you expect the independent variable to have on the dependent variable. Use the following format: If (the independent variable is manipulated in this way), then (the dependent variable will respond in this way). What will be your control for your experiment? What will be your experimental group? How many replicates or trials will you carry out, and why? Constructing a Good Hypothesis 3. Now, state your hypothesis in terms of the effect you expect the independent variable to have on the dependent variable. Use the following format: If (the independent variable is manipulated in this way), then (the dependent variable will respond in this way). Constructing a Good Experiment from a Good Hypothesis 4. What will be your control for your experiment? 5. What will be your experimental group? 6. How many replicates or trials will you carry out, and why? Instructions continued 7. Draw a quick diagram below of what your experimental set-up will look like, and label everything. Instructions continued Step three: Write out your experimental procedure Scientists need to write down everything they are going to do during an experiment. They need to include enough details that someone else can carry out the experiment in exactly the same way, just by reading the procedure and not having to consult with the scientist. Decide on and record your experimental procedure for carrying out your experiment below. You may wish to list your procedure as a series of steps to take. Be sure your procedure takes into account each of the following: •independent and dependent variables •control and experimental groups •how long, how many, etc. •method for data collection Instructions continued Step four: Set up your data table on a separate piece of paper Based on your experimental design and your procedure, you need to set up the appropriate number of rows and columns for your data table, in order to record quantitative data. Decide what you will be recording, and what you will label the rows, and what you will label the columns. Below your data table, leave some room for the recording of some qualitative data (written out in sentences). Step Five: set up your experiment according to your diagram, carry out your written procedure, and record your data (your results) in your data table. Conclusions Use the following as a guide in writing a conclusion to this lab. •Briefly explain what they lab was and what your hypothesis was. •State if your hypothesis was supported by the results, or not supported by the results? Give the reasons why or why not. •Now that you have done the experiment, what do you know now that you wish you knew before you started? Would these be things that you would change about your experimental design before doing the experiment again? •What are some possible sources of human error in your experimental results? (ONE SENTENCE). •Did the results of your experiment lead you to ask more questions about the behavior of the pill bug? What are some new hypotheses you have as tentative explanations for pill bug behavior?