Office for Research and Programs
MBL
7 MBL Street
Woods Hole, MA 02543
USA
p: 508-289-7270
f: 508.289-7934
jgitlin@ MBL.edu
WHITMAN CENTER
DIRECTORY
2014
Founded in 1888 as the Marine Biological Laboratory
LastName
Alford
Armstrong
Augustine
Baker
Balmer
Bennett
Berger
Bezanilla
Bloom
Bodznick
Bohorquez
Brady
Brangwynne
Burgess
Chang
Chen
Cohen
Colin
Colon-Ramos
Costello
Dzakpasu
Echeverry
Field
Fritzenwanker
Furlow
Gadsby
Garcia-Arraras
Gatlin
Gillis
Gladfelter
Goldman
Gonczy
Goshima
Grant
Gray
Green
Groh
Gross
Hammarlund
Hershko
Huh
Jonas
Jorgensen
Kaczmarek
Karlstrom
Kaupp
Kittelberger
Kovar
Lee
Llinas
Llobet
FirstName
Simon
Peter
George
Robert
Tim
Michael
Christopher
Magdalena
Ona
David
Diego
Scott
Clifford
David
Fred
Christopher
Lawrence
Sean
Daniel
John
Rhonda
Fabio
Christine
Jens
John
David
Jose
Jesse
James
Amy
Robert
Pierre
Gohta
Philip
Jessica
William
Alexander
Jeffrey
Marc
Avram
Yeowool
Elizabeth
Erik
Leonard
Rolf
U. Benjamin
Matthew
David
Wei-Lih
Rodolfo
Artur
StartDate
05/15/2014
06/01/2014
06/01/2014
06/01/2014
05/26/2014
07/01/2014
06/28/2014
06/23/2014
07/01/2014
05/01/2014
05/26/2014
07/01/2014
06/13/2014
06/02/2014
06/30/2014
05/26/2014
05/20/2014
06/01/2014
06/01/2014
06/15/2014
06/15/2014
05/25/2014
06/24/2014
05/01/2014
07/01/2014
05/01/2014
05/15/2014
06/14/2014
05/26/2014
06/14/2014
06/02/2014
06/16/2014
07/14/2014
06/30/2014
05/01/2014
06/22/2014
05/26/2014
06/01/2014
06/29/2014
07/07/2014
05/26/2014
06/15/2014
06/27/2014
06/18/2014
07/07/2014
07/01/2014
05/23/2014
07/13/2014
06/23/2014
05/01/2014
06/16/2014
EndDate
Room Assignment
08/15/2014
R-407
09/30/2014
L-118
08/31/2014
R-221
10/01/2014
R-220
08/30/2014
R-201,223
09/15/2014
R-213
08/16/2014
L-341
08/22/2014
L-104,105
08/25/2014
R-406
09/01/2014
R-211
08/30/2014
R-201,223
08/31/2014
R-108,109
08/03/2014
R-319,320, 321
08/30/2014
R-308
08/10/2014
R-308
08/30/2014
R-201,223
09/05/2014
R-210
08/28/2014
R-301
08/15/2014
R-219
09/01/2014
R-301
08/02/2014
R-322
08/30/2014
R-201,223
09/04/2014
R-205/215
05/19/2014
R-108
09/30/2014
Lb NXR G12
09/21/2014
L-121
08/15/2014
L-341
08/17/2014
R-206
08/08/2014
Lb-257A
08/17/2014
R-319,320, 321
08/29/2014
R-305/307
07/28/2014
L-118
08/24/2014
L-104,105
09/06/2014
R-326
05/30/2014
R-108
08/09/2014
R-322
08/30/2014
R-201,223
09/15/2014
R-211
08/30/2014
L-341
08/28/2014
R-311
08/30/2014
R-201,223
09/15/2014
R-213
07/27/2014
R-310
09/15/2014
R-213
08/23/2014
R-421
08/30/2014
R-312
08/30/2014
R-201,223
08/02/2014
L-104,105
08/15/2014
L-104,105
10/31/2014
R-207,208,209
08/04/2014
Lb NXR G12
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Lowe
Maienschein
Malchow
Martinez-De Luna
Mease
Mensinger
Miller
Mitchison
Morfini
Nakamura
Oakey
Pant
Parker
Pereda
Rome
Rosa-Molinar
Rose
Rosen
Rosenthal
Rost
Selvin
Sloboda
Sluder
Song
Springer
Tamm
Terasaki
Treistman
Vale
Watanabe
Waterman
Wilhelm
Wu
Younger
Zimmerberg
Zottoli
Christopher
Jane
Robert
Reyna
Rebecca
Allen
Andrew
Timothy
Gerardo
Tetsuya
John
Harish C
Roy
Alberto
Lawrence
Eduardo
Robert
Michael
Joshua
Benjamin
Paul
Roger
Greenfield
Yuyu
Timothy
Sidney
Mark
Steven
Ron
Shigeki
Clare
Jim
Hao
Meg
Joshua
Steven
09/01/2014
05/17/2014
06/17/2014
05/26/2014
05/26/2014
06/15/2014
06/01/2014
06/22/2014
07/01/2014
06/20/2014
06/15/2014
06/15/2014
06/16/2014
05/23/2014
06/01/2014
06/28/2014
06/18/2014
06/16/2014
06/20/2014
05/23/2014
06/15/2014
06/20/2014
05/01/2014
05/26/2014
05/30/2014
06/15/2014
05/15/2014
06/01/2014
06/15/2014
05/26/2014
06/10/2014
06/14/2014
06/16/2014
05/26/2014
06/15/2014
05/01/2014
09/30/2014
09/16/2014
08/23/2014
08/30/2014
08/30/2014
08/25/2014
09/30/2014
08/31/2014
09/01/2014
08/31/2014
08/15/2014
09/03/2014
08/01/2014
09/01/2014
08/31/2014
08/03/2014
07/31/2014
06/28/2014
08/10/2014
08/30/2014
07/25/2014
08/30/2014
10/01/2014
08/30/2014
09/07/2014
10/15/2014
10/31/2014
09/30/2014
08/31/2014
08/20/2014
08/05/2014
08/16/2014
08/01/2014
08/30/2014
09/07/2014
08/31/2014
Library, L-228
MRC-306
R-201,223
R-201,223
MRC-306
R-220
R-205,215
R-108,109
MRC-306
R-206
R-326
R-319,320,321
R-201,223
R-313
L-208
R-401,423
R-319,320,322
R-219
R-201,223
R-322
L-121
R-308
R-201,223
R-305
R-113
R-113
R-219
R-319,320,321
R-201,223
R-305, 307
R-319,320,322
R-319, 320, 321
R-201,223
R-309
R-213
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Simon Alford
MBL Association:
PI-MBL Research Awardee
Institution Contact Information:
University of Illinois at Chicago
Biological Sciences
840 W Taylor St
Chicago IL 60607
Email:
sta@uic.edu
Dates at MBL:
5/15/2014 - 8/15/2014
Research Title:
Research
Description:
Temporo-spatial analysis of the synaptic vesicle pool
Room Assignment: R-407
Neurons interconnect at synapses, which function by releasing packets of neurotransmitter from vesicles located adjacent to
fusion sites in pools defined physiologically by their probability of fusion: the readily-releasable, recycling, and reserve pools. After
fusion, vesicle membrane is retrieved and proteins are sorted back into appropriate vesicle pools. This process of endocytosis
follows one of at least two possible fusion modes after low to medium frequency activation: full vesicle fusion, where the vesicle
fully collapses into the presynaptic membrane, or kiss-and-run, where it transiently fuses and the fusion pore reseals. These two
modes may lead to very different endocytic pathways, while a third mechanism – bulk endocytosis – may follow high frequency
activity. Though endocytosis is required for neural function, little is known of the processes that lead to different endocytic modes,
which protein partners are utilized in different modes, and which of the physiologically defined pools of synaptic vesicles the
endocytosed vesicle reenters. To address these questions, we will use the unique accessibility of the lamprey reticulospinal (RS)
synapse, which enables molecular perturbations and a variety of imaging modalities to be applied.
Peter Armstrong
MBL Association:
Principal Investigator
Institution Contact Information:
University of California
Molecular and Cellular Biology
One Shields Ave
Davis CA 95616
Email:
pbarmstrong@ucdavis.edu
Dates at MBL:
6/1/2014 - 9/30/2014
Research Title:
Research
Description:
Innate immunity in long-lived animals
Room Assignment: L-118
I will investigate the processes by which the important microbial toxin lipopolysaccharide (LPS, endotoxin) is processed by longlived animals.
1
George Augustine
MBL Association:
Principal Investigator & Grass Imaging Awards Director
Institution Contact Information:
Duke University Medical Center
Neurobiology
359 Bryan Research Building
Research Dr.
Durham NC 27710
Email:
georgea@neuro.duke.edu
Dates at MBL:
6/1/2014 - 8/31/2014
Research Title:
Research
Description:
Molecular mechanisms of neurotransmitter release
Room Assignment: R-221
Our lab uses the squid giant synapse as a model system to understand the mechanisms involved in communication between brain
cells.
Robert Baker
MBL Association:
Principal Investigator
Institution Contact Information:
New York University Langone Medical Center
Physiology and Neuroscience
550 First Ave
New York, NY NY 10016
Email:
robert.baker@nyumc.org
Dates at MBL:
6/1/2014 - 10/1/2014
Research Title:
Research
Description:
Analysis of hindbrain and cerebellar development in zebrafsih
Room Assignment: R-220
We plan to study the development of vestibular afferent and efferent neurons in zebrafish that are hypothesized to produce eye
movement following visual and vestibular stimulation. These neurons will be identified by stable transgenic markers and studied
between 3-5 days post fertilization. This work is is designed to provide a basic description of the structure and function of
vestibular neural circuits essential for zebrafish swimming, predation and in fact survival.
2
Tim Balmer
MBL Association:
PI-Grass Fellow
Institution Contact Information:
Georgia State University
Neuroscience Institute
PO Box 5030
Atlanta GA 30302
Email:
tbalmer2@student.gsu.edu
Dates at MBL:
5/26/2014 - 8/30/2014
Research Title:
Research
Description:
The contribution of perineuronal nets to regulation of neuronal activity
Room Assignment: R-201,223
Perineuronal nets (PNNs) are specialized condensations of extracellular matrix that surround the somata of neurons throughout
the brain. Because PNNs surround fast-spiking neurons and are highly negatively charged, it has been proposed that they act as
a buffering system for cations, supporting fast and precise action potential generation. Electrophysiological recordings paired with
enzymatic digestion of PNNs will allow me to test the role of PNNs in supporting fast-spiking and timing accuracy, and buffering
potassium ions that contribute to fast repolarization of action potentials. These experiments will identify how PNNs contribute to
neuronal function and how PNNs could contribute to pathologies of neuronal activity such as epilepsy.
Michael Bennett
MBL Association:
Principal Investigator
Institution Contact Information:
Albert Einstein College of Medicine
Neuroscience
1300 Morris Park Ave
Bronx NY 10461
Email:
mbennett@aecom.yu.edu
Dates at MBL:
7/1/2014 - 9/15/2014
Research Title:
Research
Description:
Evolution of electrical coupling
Room Assignment: R-213
Electrical coupling by way of gap junctions has evolved at least twice. In vertebrates, gap junctions are formed by connexins, a
gene family of 21 members in humans. Connexins first appear in ascidians and are not found in less derived deuterostomes
including branchiostomes or in protostomes. In protostomes gap junctions are formed by pannexins/innexins, a gene family
expressed in Coelenterates, Cniderians, Platyhelminthes, Nematodes, Molluscs, and Arthropods. Pannexins are also expressed in
Branchiostomes, ascidians, and vertebrates. In vertebrates, pannexins are not known to form gap junctions and electrically couple
cells expressing them endogenously. In mammals pannexins are thought to form “hemichannels” that directly connect cell
cytoplasm and external milieu. Neither connexins or pannexins are expressed in echinoderms or in a primitive eukaryote,
Tricoplax. We hope to find a collaborator to examine coupling in Arbacia, Brachiostoma, Saccoglossus, and Tricoplax.
3
Christopher Berger
MBL Association:
PI-MBL Research Awardee
Institution Contact Information:
University of Vermont
Molecular Physiology & Biophysics
149 Beaumont Ave.
E217 Given, Collegeof Medicine
Burlington VT 05405
Email:
cberger@uvm.edu
Dates at MBL:
6/28/2014 - 8/16/2014
Research Title:
Research
Description:
Single Molecule Imaging of Tau Dynamics in Isolated Squid Axoplasm
Room Assignment: L-341
Axonal transport is a critical process in nerve cells in which biomolecules and cellular organelles manufactured in the cell body are
moved along microtubule tracks by motor proteins (e.g., kinesin) to the distal regions of the axon, a long cellular extension that
interacts with other neurons and effector cells such as muscle. Defects in any one of the protein components in the axonal
transport machinery result in serious and often lethal neurodegenerative diseases, including Alzheimer’s, Huntington’s, and ALS.
These neurodegenerative disorders affect millions of Americans with a staggering economic toll on health care costs, but we
unfortunately still do not fully understand the biological mechanisms that lead to these pathological states. Tau is a neuronalspecific microtubule associated protein implicated in a number of these neurodegenerative diseases. It is a diverse molecule
known to be conformationally dynamic, adopting multiple structural states that define a variety of different functions within the
neuron, including stabilization of microtubules and modulating the axonal transport process. However, despite the importance of
tau’s structural dynamics to its function, the structure/function relationships within this molecule remain poorly defined. Previous in
vitro work in our lab has shown that tau exists in a dynamic equilibrium on the microtubule surface between static and mobile
(diffusive) states, where the static state can disrupt axonal transport. At the MBL we will extend our cutting-edge quantitative
single molecule fluorescence imaging techniques to study the dynamics of tau and their effects on axonal transport in the classic
isolated squid axoplasm system, recapitulating the intracellular environment within the neuron.
Magdalena Bezanilla
MBL Association:
PI-MBL Research Awardee
Institution Contact Information:
University of Massachusetts Amherst
Biology
221 Morrill South
611 N. Pleasant Street
Amherst MA 01003
Email:
bezanilla@bio.umass.edu
Dates at MBL:
6/23/2014 - 8/22/2014
Research Title:
An unconventional myosin mediates cross talk between the actin and microtubule
cytoskeletons _
_____________________________________________________________________
In
many cells, placement of the cell division plane often affects cell fate specification. In plants this is particularly relevant. Plants
Research
Description:
Room Assignment: L-104,105
are surrounded by a rigid cell wall; thus, patterns of cell division and expansion ultimately dictate cell shape. My lab has
discovered a link between microtubules in the phragmoplast, the structure responsible for cell plate formation, and cell plate
deposition during cytokinesis. We propose that peripheral phragmoplast microtubules loaded with myosin VIII on their plus ends
use actin filaments between the phragmoplast edge and the cell cortex to guide proper phragmoplast expansion. This summer we
aim to validate the model and gain a molecular understanding for myosin VIII localization throughout mitosis and cytokinesis.
4
Ona Bloom
MBL Association:
PI-MBL Research Awardee
Institution Contact Information:
Feinstein Institute for Medical Research; Hofstra North Shor
Autoimmune/Physical Med & Rehab
350 Community Drive
1FR
Manhasset NY 11030
Email:
obloom@nshs.edu
Dates at MBL:
7/1/2014 - 8/25/2014
Research Title:
Research
Description:
Defining the conserved molecular pathways underlying successful regeneration after SCI
Room Assignment: R-406
The ability to promote successful recovery after spinal cord injury (SCI) will likely derive from identification of biological processes
that accompany both success and failure to regenerate central nervous system (CNS) tissue in species where regenerative
capacity ranges from limited to robust. In contrast to mammals, other vertebrates, such as jawless (lamprey) and jawed
vertebrates (salamander), achieve significant spontaneous anatomical and functional recovery after SCI. The mechanisms that
support regeneration in these animals are largely unknown, limiting our ability to promote regeneration in mammals. Here, our
goal is to bridge that gap using a comparative genomics approach to identify conserved molecular responses underlying
successful regeneration after SCI using parallel injury models, data collection and analysis methods, and common molecular
manipulations in salamander and lamprey.
David Bodznick
MBL Association:
Principal Investigator
Institution Contact Information:
Wesleyan University
Biology
45 Wyllys Ave
Middletown CT 06459
Email:
dbodznick@wesleyan.edu
Dates at MBL:
5/1/2014 - 9/1/2014
Research Title:
Research
Description:
Did the cerebellum evolve from a cerebellum like sensory structure?
Room Assignment: R-211
To develop a novel motor learning paradigm in fish that demonstrates continuity in functional algorithm between cerebellum and
cerebellum-like structures. In particular, a negative motor image analogous to the negative sensory image that is diagnostic of the
sensory adaptive filter mechanism we have studied in the cerebellum-like sensory nuclei in sharks, skates and other fishes. Much
of this work has been the result of a long-term collaboration between Professors Bodznick and Montgomery.
5
Diego Bohorquez
MBL Association:
PI-Grass Fellow
Institution Contact Information:
Duke University Medical Center
Medicine
300 Research Dr.
Sands Building 334
Durham NC 27710
Email:
diego.bohorquez@duke.edu
Dates at MBL:
5/26/2014 - 8/30/2014
Research Title:
Research
Description:
Function of a gut-brain neural circuit modulating satiety
Room Assignment: R-201,223
Our gut is thought to transmit sensory information to the brain through the paracrine action of hormones. This is because
enteroendocrine cells, the epithelial sensors of the intestine, are thought to lack synaptic connections with nerves. However, I
recently discovered a physical connection between enteroendocrine cells and underlying sensory neurons. This neuro-epithelial
junction may constitute the first point of integration between food sensed in the gut and satiety perceived in the brain. Hence, my
objective for the Grass Fellowship would be to study the function of this enteroendocrine cell-neuron circuit by documenting its
ultrastructure and electrical properties.
Scott Brady
MBL Association:
Principal Investigator
Institution Contact Information:
University of Illinois at Chicago
Anatomy and Cell Biology
808 S Wood St Rm 578
MC 512 Chicago IL
Email:
stbrady@uic.edu
Dates at MBL:
7/1/2014 - 8/31/2014
Research Title:
Research
Description:
Molecular mechanisms of fast axonal transport
Room Assignment: R-108,109
Neuronal growth, maintenance and regeneration are critically dependent on delivery of materials from cell body to the nerve fiber
and terminal. Motor proteins that were first described at the MBL using the squid as a model organism mediate this process,
known as fast axonal transport. We use the giant axon of the squid and digital video microscopy to study how these motor
proteins work and how they are regulated in neurons. These studies are important for understanding the basic biology of the
neuron and have provided important insights into molecular mechanisms underlying neurodegenerative diseases like ALS as well
as Huntington's, Alzheimer's and Parkinson's disease.
6
Clifford Brangwynne
MBL Association:
Principal Investigator
Institution Contact Information:
Princeton University
Chemical and Biological Engineering
301 Hoyt Laboratory
William Street
Princeton NJ 08544
Email:
cbrangwy@princeton.edu
Dates at MBL:
6/13/2014 - 8/3/2014
Room Assignment: R-319,320, 321
Research Title:
Research
Description:
David Burgess
MBL Association:
Principal Investigator
Institution Contact Information:
Boston College
Biology
Higgins Hall
140 Commonwealth Ave
Chestnut Hill MA 02467
Email:
david.burgess@bc.edu
Dates at MBL:
6/2/2014 - 8/30/2014
Research Title:
Research
Description:
Control of spindle and division plane positioning
Room Assignment: R-308
Four summers ago, Fred Chang and I shared a lab at the MBL and began a very fruitful collaboration. With his postdoc Nico
Minc, an engineer learning biology, we devised microfabricated chambers used to change the shape of cells in a predictable
fashion. Our goal was to design shapes that would comment on the rules that position the cleavage furrow in sea urchin eggs, a
system my lab had been studying for over 30 years. By emulating Rappaport's famous single cell shape change experiments
using the power of scale, we thought we could further comment on the regulation of cytokinesis. What ensued was a study on the
control of nuclear centering and spindle positioning published in Cell. We propose to continue this work by performing live cell
imaging of the location and dynamics of microtubules and actin filaments in shaped cells and cell extracts.
7
Fred Chang
MBL Association:
Principal Investigator
Institution Contact Information:
Columbia University
Microbiology
701 168th St.
New York NY 10032
Email:
fc99@columbia.edu
Dates at MBL:
6/30/2014 - 8/10/2014
Research Title:
Research
Description:
Positioning of the cell division plane
Room Assignment: R-308
Cytokinesis is the process in which a cell is divided into two. One of the key questions in cytokinesis is how the cell decides where
to divide. The proper selection of the division plane is critical for the embryonic development and organization of cells in the body.
The mitotic spindle is thought to control where the cell divides. Models have been proposed in which the spindle microtubules
transport signals; to the right location on the cell surface. The molecular details of this process and even the precise role of
microtubules in this process are not well understood. The shape of the cell may guide division patterns during development. We
have recently found a way of altering cell shape by introducing cells into tiny wells of different shapes, forming cells for instance
the shape of squares, triangles and even stars. By combining these experiments in sea urchin cells with computer modeling, we
hope to test models of cell division.
Christopher Chen
MBL Association:
PI-Grass Fellow
Institution Contact Information:
Albert Einstein College of Medicine
Neuroscience
1410 Pelham Pkwy South, Albert
Kennedy Center Room 506
Bronx NY 10461
Email:
Dates at MBL:
5/26/2014 - 8/30/2014
Room Assignment: R-201,223
Research Title:
Research
Description:
Corticothalamic modulation of cerebellar gain
It is well known that attention sharpens and increases sensory perception. Similarly, attention is critical for the completion of
complex motor tasks, but the neural mechanisms of this are unknown. In sensory systems, corticothalamic modulation of thalamic
activity is a significant substrate for these increases in perception. Comparable circuits also exist in the motor system, suggesting
that corticothalamic inputs at the thalamus can increase the gain of motor systems. Moreover, there are significant inputs from the
frontal cortex to the origins of these corticothalamic axons, suggesting that executive control areas may exert control over
cerebellar outputs at the thalamus. Successful completion of this project will extend attentional mechanisms to the motor system
and advance our understanding of corticothalamic modulation of subcortical computations.
8
Lawrence Cohen
MBL Association:
Principal Investigator
Institution Contact Information:
Korea Institute of Science and Technology/ Yale
Center for Functional Connectomics/Physiology
Hwarangno 14-gil 5
Seongbuk-gu
Seoul Seoul 136-791
Email:
lawrence.cohen@yale.edu
Dates at MBL:
5/20/2014 - 9/5/2014
Research Title:
Research
Description:
Optical studies of neuron activity and organization
Room Assignment: R-210
The proposed experiments are directed toward understanding how the brain accomplishes odor recognition. The experiments
involve 2-photon microscopic measurements of calcium changes in neurons from in vivo mouse preparations. With this technique
we can follow the activity of hundreds of neurons simultaneously. We hope that this capability will increase our understanding of
these brain functions.
Sean Colin
MBL Association:
Principal Investigator
Institution Contact Information:
Roger Williams University
Environmental Science
One Old Ferry Road
Bristol RI 02809
Email:
scolin@rwu.edu
Dates at MBL:
6/1/2014 - 8/28/2014
Research Title:
Research
Description:
Effects of turbulence on the feeding ecology of the lobate ctenophore Mnemiopsis leidyi
Room Assignment: R-301
The lobate ctenophore Mnemiopsis leidyi is a voracious planktonic predator whose global distribution is expanding. In order to
understand the types of environmental conditions in which M. leidyi is capable of thriving we will investigate the effects of different
turbulence environments on M. leidyi. We will use a combination of laboratory experimentation and field sampling to quantify how
different levels of turbulent mixing affect the feeding behavior and predatory impact of M. leidyi.
9
Daniel Colon-Ramos
MBL Association:
PI-MBL Research Awardee
Institution Contact Information:
Yale University
Cell Biology
295 Congress Avenue
BCMM 436B CT 06510
Email:
daniel.colon-ramos@yale.edu
Dates at MBL:
6/1/2014 - 8/15/2014
Research Title:
Research
Description:
An integrated system to monitor complex tissues at single-cell resolution
Room Assignment: R-219
We have recently developed a prototype system for fast, long-term imaging and analysis of single-cell behaviors in complex
tissues and whole embryos. We aim to bring this innovative and unique system to maturity and to the hands of the research
community as a powerful and versatile tool for single-cell studies in complex, differentiating populations. During our summer
tenure at Woods Hole we will seek to achieve two complimentary goals: 1) Establish collaborations with resident and visiting
scientists to apply the versatility of this new instrumentation to different biological questions of interest. In particular, we have
established a formal collaboration with visiting scientist Steve Treistman, and the University of Puerto Rico lab at Woods Hole.
Woods Hole is an ideal setting to get this innovative system to the hands of the research community, and that is one of the primary
goals of our visit. 2) The four investigators listed in this proposal will collaborate during the summer months to bring their
neurobiology and cell biology expertise (Daniel Colón-Ramos), imaging expertise (Hari Shroff) and single cell tracking expertise
(Zhirong Bao) and developmental biology expertise (William Mohler) to bear in improving the current system.
John Costello
MBL Association:
Principal Investigator
Institution Contact Information:
Providence College
Biology
River Ave.
Providence RI 02918
Email:
costello@providence.edu
Dates at MBL:
6/15/2014 - 9/1/2014
Research Title:
Quatitatively evaluating the role of in situ turbulence on predation by the lobate ctenophore Mnemiopsis
leidyi
Research
Description:
The ctenophore Mnemiopsis leidyi is an important planktonic predator whose predatory impacts
have been associated with profound alterations in planktonic community structure both in its endemic and invaded ranges. Our
ability to predict its impact within planktonic communities depends upon the depth of our understanding of its predatory
mechanisms. Laboratory studies have provided us with an understanding of the mechanisms by which Mnemiopsis encounters
different types of prey and the efficiency with which it captures them. Yet, despite advances in the descriptions of ctenophore
predation mechanisms, the unpredictability of field diet selection patterns demonstrates that our current understanding is
incomplete.
Room Assignment: R-301
We propose a synthetic approach to resolve these shortcomings that utilizes the complimentary strengths of both laboratory and
field methods.
10
Rhonda Dzakpasu
MBL Association:
PI-MBL Research Awardee
Institution Contact Information:
Georgetown University
Physics
506 Reiss
37th and O St. NW
Washington DC 20057
Email:
dzakpasu@physics.georgetown.edu
Dates at MBL:
6/15/2014 - 8/2/2014
Research Title:
How Aβ pathology modulates network activity within the entorhinal cortex-hippocampus microcircuit in
an Alzheimer’s mouse model
Research
Description:
Alzheimer’s disease (AD) is a progressive, age-related neurodegenerative disorder characterized by significant impairment in
declarative memory. AD accounts for 60-80% of dementia cases in the United States and is the 6th leading cause of death in the
country. The largest overall risk factor for AD is aging. Mounting evidence indicates that AD pathology develops in a stereotypical
form with the accumulation of extracellular amyloid  (Aβ) plaques and intraneuronal neurofibrillary tangles (NFTs), as well as
Room Assignment: R-322
neuronal loss that initially occurs in layer II of entorhinal cortex. However, mechanisms that underlie the network effects and
propagation of pathology from the primary site of pathological origin remain to be elucidated. We will study deficits in network
activity from specific neural populations within an in vitro slice mouse model of AD via the following two aims. Aim 1: To
investigate how A modulates network temporal dynamics within the entorhinal-hippocampus circuit using an amyloidogenic
mouse model. Aim 2: Investigate the impact of Aon synaptic plasticity within a network of cultured hippocampal neurons.
Fabio Echeverry
MBL Association:
PI-Grass Fellow
Institution Contact Information:
Center of Advanced European Studies and Research (caesar)
Molecular Sensory Systems
Ludwig-Erhard-Allee 2
Bonn NRW 53175
Email:
fabio.echeverry-bautista@caesar.de
Dates at MBL:
5/25/2014 - 8/30/2014
Research Title:
Research
Description:
Physiological Role of cNMP-modulated Channels on Scallop Ciliary Photoreceptors
Room Assignment: R-201,223
Ciliary photoreceptors of the scallop (Pecten irradians) respond to light with a hyperpolarization. Patch-clamp studies have shown
that intracellular dialysis of cGMP opens light-activated K+ channels. In order to identify the light-activated channel, I have
molecularly identified different ion channels that harbor a cyclic-nucleotide binding domain (HCN, CNG and ERG channels),
expressing in the ciliary photoreceptors of Pecten. I aim to study the physiological role played by these ion channels in native
ciliary photoreceptors of the scallop by using the patch-clamp technique. This approach will help to deeper understand the
different photo-transduction pathways present through the ciliary photoreceptor lineage.
11
Christine Field
MBL Association:
PI-MBL Research Awardee
Institution Contact Information:
Harvard Medical School
Systems Biology WA 536
200 Longwood Av
Boston MA 02115
Email:
christine_field@hms.harvard.edu
Dates at MBL:
6/24/2014 - 9/4/2014
Research Title:
Research
Description:
Cytokinesis signaling in a cell free system
Room Assignment: R-205/215
We have developed methods for reconstituting cytokinesis signaling in a cell free system derived from Xenopus eggs. Overlap
zones between microtubule asters recruited various signaling complexes, including the Aurora B complex/CPC, to form midzonelike assemblies. On a supported lipid bilayer underlying these midzones, several cleavage furrow markers, including F-actin, RhoA
and Anillin were selectively recruited. Aurora B activity was required for midzone formation and for recruitment of furrow
components to the lipid bilayer. In 2104 we will start to probe mechanisms involved in these assembly and signaling processes.
We will test role of the kinesin, Kif20A in transporting Aurora B complex/CPC to the center of midzones. We will develop systems
for artificially localizing Aurora B complex/CPC on supported lipid bilayers, and ask if downstream cleavage components become
localized to lipid bilayers in response.
Jens Fritzenwanker
MBL Association:
Principal Investigator
Institution Contact Information:
Hopkins Marine Station of Stanford University
Hopkins Marine Station
120 Oceanview Blvd
Pacific Grove CA 93950
Email:
jens.fritzenwanker@web.de
Dates at MBL:
5/1/2014 - 5/19/2014
Research Title:
Research
Description:
Bilaterian body plan evolution
Room Assignment: R-108
My research focuses on general bilaterian body plan evolution with focus on early evolution of deuterostomes. I am especially
interested in the evolution of the bilaterian trunk and the evolutionary plasticity of nervous systems. I am addressing my research
questions by analyzing and comparing gene regulatory networks."
12
John Furlow
MBL Association:
PI-MBL Research Awardee
Institution Contact Information:
University of California, Davis
Neurobiology, Physiology and Behavior
One Shields Avenue
Davis CA 95616
Email:
jdfurlow@ucdavis.edu
Dates at MBL:
7/1/2014 - 9/30/2014
Research Title:
In vivo analysis of thyroid hormone receptor signaling during development using emerging genetic
technologies in Xenopus laevis and Xenopus tropicalis
Research
Description:
We are applying emerging powerful genetic approaches in Xenopus laevis and Xenopus tropicalis available at the Xenopus
National Resource at the MBL to reduce the expression of thyroid hormone receptor subtypes, and test their roles during early
development and thyroid hormone dependent metamorphosis. These studies will contribute to our understanding of tissue specific
actions of hormones in a developmental context, using this highly evolutionarily conserved endocrine signaling system. Another
important aspect of our work is determining the effect of environmental chemicals on proper thyroid hormone signaling in
vertebrate development. We are further applying the available genetic technologies now available in the organism to produce
tadpoles that express easily measurable, hormone responsive reporter genes. This will allow us to detect proper temporal and
spatial thyroid hormone action in vivo, in response to both endogenous hormones and potential environmental contaminants.
Room Assignment: Lb NXR G12
David Gadsby
MBL Association:
Principal Investigator
Institution Contact Information:
Rockefeller University
Lab Cardiac/Membrane Physiology
1230 York Ave
New York NY 10065
Email:
gadsby@rockefeller.edu
Dates at MBL:
5/1/2014 - 9/21/2014
Research Title:
Research
Description:
Mechanisms of function and dysfunction of the Na, K-ATPase pump
Room Assignment: L-121
We study how sodium and potassium ions, and recently also protons, move through the Na/K-ATPase pump. The Na/K pump
opens and closes gates to allow access to its ion-binding sites alternately from only the cytoplasmic or the extracellular side of the
membrane. We use electrical recordings to investigate the conformational changes of the Na/K pump that control this orchestrated
series of steps that effects ATP-driven active transport of sodium and potassium ions across the surface membranes of all animal
cells. This transport generates the transmembrane gradients of sodium and potassium concentration on which cell life depends.
We recently found that protons hijack a subset of Na/K pump conformations during normal sodium and potassium transport and
hop between a pair of protonatable residues to cause an inward current of protons. The physiological and/or pathological
consequences of this Na/K pump-mediated cellular proton influx remain to be determined.
13
Jose Garcia-Arraras
MBL Association:
PI-MBL Research Awardee
Institution Contact Information:
University of Puerto Rico
Biology
Box 23360
UPR Station
San Juan PR 00931-3360
Email:
jegarcia@hpcf.upr.edu
Dates at MBL:
5/15/2014 - 8/15/2014
Research Title:
Research
Description:
Regeneration of the nerve ring in holothurans: cellular and molecular studies
Room Assignment: L-341
The long-term goal of our project is to determine the cellular and molecular bases of echinoderm regenerative capacities. During
the summer period we will focus on studying regeneration in the holothurian species, Sclerodactyla briareus. Our project will
accomplish two different but related goals. First, it will serve to determine the cellular and molecular events associated with the
regeneration of the nerve ring, the structure that is the major component of the central nervous system of echinoderms. Second, it
will serve to establish new tools to study a species that is commercially available and thus can be easily obtained by national and
international researchers. Specifically we plan to develop an RNAi methodology for gene knockdown in regenerating holothurians.
Jesse Gatlin
MBL Association:
PI-MBL Research Awardee
Institution Contact Information:
University of Wyoming
Molecular Biology (#3944)
1000 E. University Ave
Laramie Wyoming 82071
Email:
jgatlin@uwyo.edu
Dates at MBL:
6/14/2014 - 8/17/2014
Research Title:
Research
Description:
Room Assignment: R-206
Spindle assembly and positioning in limiting cytoplasmic volumes
In order to accurately segregate its copied chromosomes, a dividing cell must first assemble a mitotic spindle, a microtubulebased structure that attaches to and physically pulls apart sister chromosomes. This structure is often assembled at or moved
close to the cell center prior to anaphase onset, ensuring proper positioning of the division plane. Many of the molecular players
involved in spindle assembly have now been identified, but it remains unclear how they self-assemble to form a steady-state
structure with a constant length and characteristic shape. Even less is known about how spindles find the center of cells,
particularly large ones. The objective of our MBL research is to characterize the effects of limiting cytoplasmic volumes on both
spindle assembly and spindle positioning. We have recently developed an experimental platform that combines microfluidics and
cell-free extracts to investigate spindle size regulation during early development. For our summer research, we plan to build upon
this platform to determine how limiting cytoplasmic volumes might affect spindle shape and to examine the biomechanics involved
in spindle positioning.
14
James Gillis
MBL Association:
PI-MBL Research Awardee
Institution Contact Information:
Dalhousie University
Biology
Life Science Centre
1355 Oxford Street
Halifax Nova Scotia B3H 4R2
Email:
andrew.gillis@dal.ca
Dates at MBL:
5/26/2014 - 8/8/2014
Research Title:
Experimental investigations of craniofacial development and cartilage repair in the little skate, Leucoraja
erinacea
Research
Description:
My research program uses cartilaginous fishes (sharks, skates, rays and holocephalans) as models for the development of the
skeleton and sensory nervous system of jawed vertebrates. Cartilaginous fishes have retained a number of primitive anatomical
conditions, and, as the sister group the bony fishes (including model systems like mouse and zebrafish), occupy a critical
phylogenetic position for studies aiming to understand the nature of the last common ancestor of jawed vertebrates. My 2014
summer research will use embryos of the little skate, Leucoraja erinacea, to address the following questions: 1) What is the
molecular basis of gill arch appendage patterning in cartilaginous fishes, and were gill arch appendages evolutionary antecedents
to fins/limbs; and 2) What are the embryonic origins of the spiracular sense organ of cartilaginous fishes, and is this organ
homologous with the paratympanic organ of birds and other tetrapods? This research will shed light on the evolutionary origin of
the jawed vertebrate body plan, and will yield new data on fundamental processes in developmental biology (e.g. axial patterning,
appendage development and sensory receptor cell differentiation).
Room Assignment: Lb-257A
Amy Gladfelter
MBL Association:
Principal Investigator
Institution Contact Information:
Dartmouth College
Biological Sciences
78 Life Sciences Center
Hanover NH 03755
Email:
amy.gladfelter@dartmouth.edu
Dates at MBL:
6/14/2014 - 8/17/2014
Research Title:
Research
Description:
Room Assignment: R-319,320, 321
Local control of cytokinesis analyzed in a cell-free system
We use Xenopus egg extracts and supported synthetic lipid bilayers to monitor the recruitment of proteins involved in cleavage
furrow formation (RhoA, anillin and septins) in response to signals from the cell cycle and microtubules. Our goals for this summer
are to determine to role of lipid membrane composition and the cytoskeleton in restricting the scale and position of furrow protein
recruitment. This system further extends our collaboration with the MBL Cell Dynamics Center on septin polymerization and
dynamics to a vertebrate system that is amenable to single molecule and orientational (by polarized fluorescence) imaging. This
work addresses two fundamental problems: 1) how cells locally signal the position their cleavage plane and 2) how septin
polymers assemble at the plasma membrane. Additionally, we aim to develop mRNA expression for the Xenopus interphase
extract to expand and make analysis of a wider array of factors more feasible.
15
Robert Goldman
MBL Association:
Principal Investigator
Institution Contact Information:
Northwestern Univ. Medical School
Cell and Molecular Biology
303 E. Chicago Ave.
Ward Building W11-145
Chicago IL 60611
Email:
r-goldman@northwestern.edu
Dates at MBL:
6/2/2014 - 8/29/2014
Research Title:
Research
Description:
Nuclear Lamins -- structure and function in nuclei and meiotic spindles
Room Assignment: R-305/307
We are studying the structure and function of intermediate filaments.
Pierre Gonczy
MBL Association:
Principal Investigator
Institution Contact Information:
Swiss Federal Institute of Technology, Lausanne (EPFL)
SV1526
Station 19
CH-1015 Lausanne
Switzerland
Email:
pierre.gonczy@epfl.ch
Dates at MBL:
6/16/2014 - 7/28/2014
Research Title:
Research
Description:
Investigating mechanisms of centriole elimination
Room Assignment: L-118
We plan notably to explore the fate of exogenously provided centrioles in starfish and sea urchin zygotes, as well as the patterns
of centriole inheritance in horseshoe crab embryos.
16
Gohta Goshima
MBL Association:
PI-MBL Research Awardee
Institution Contact Information:
Nagoya University
Division of Biological Science, Graduate School of
Science
Furo-cho, Chikusa-ku
Nagoya Aichi 464-8602
Email:
goshima@bio.nagoya-u.ac.jp
Dates at MBL:
7/14/2014 - 8/24/2014
Research Title:
Research
Description:
Organelle transport in plant cells
Room Assignment: L-104,105
Transport of intracellular cargo such as organelles, vesicles, or proteins along the microtubule is critical for cell function or
organisation. In animal cells, the retrograde transport along microtubules is executed by the cytoplasmic dynein motor.
Interestingly, although land plants lack cytoplasmic dynein, we recently observed retrograde transport of chloroplasts and
mitochondria, two large organelles in the cytoplasm, in the moss Physcomitrella patens. We aim to identify the motor protein
responsible for the retrograde transport of these organelles. We will perform time-lapse observation of organelle movement in the
absence of the kinesin-type motor proteins in moss cells and perform in vitro motility assays using purified motors.
Philip Grant
MBL Association:
Principal Investigator
Institution Contact Information:
National Institutes of Health
LNC NINDS
Bldg 49 Rm 2A35
9000 Rockville Pike
Bethesda MD 20892
Email:
grantp@ninds.nih.gov
Dates at MBL:
6/30/2014 - 9/6/2014
Research Title:
Research
Description:
Topographic regulation of cytoskeletal protein phosphorylation in the squid giant fiber system
Room Assignment: R-326
We are interested in the factors regulating the synthesis, assembly,organization and function of cytoskeletal proteins in nerve
cells. We find that these proteins, though synthesized in the nerve cell body, are only phosphorylated and assembled in the axon.
Phosphorylation of these proteins changes their stability, organization and function. The question is why and how is this
compartmentalization regulated? The giant fiber system of the squid possesses giant nerve cells which can be easily separated
into axon and cell body and studied biochemically. Several enzymes, e.g., kinases and phosphatases, are principally involved and
we are studying their relative activities, mutual interactions, localizations and response to various signaling systems. In this way
we hope to understand the metabolic network that determines the organization and function of these proteins in the nerve cell in
normal and in neurodegenerative disorders in which the process is deregulated.
17
Jessica Gray
MBL Association:
Principal Investigator
Institution Contact Information:
Harvard Medical School
Systems Biology
200 Longwood Avenue
Boston MA 02115
Email:
jessica_gray@hms.harvard.edu
Dates at MBL:
5/1/2014 - 5/30/2014
Research Title:
miRNAs in hemichordate development: Insights into the evolution of miRNA function
Research
Description:
miRNAs act as post-transcriptional regulators of gene expression networks in a number of developmental processes, multiple
evolutionary expansions of miRNAs are associated with increasing complexity. Despite growing evidence for developmental
miRNAs in model organisms and genome-wide small RNA studies in numerous species, data is lacking for a functional role of
miRNAs in the development of non-model organisms. The question is can evolution of miRNA targets and functions have driven
the evolution of developmental pathways or if they are instead uniquely regulated in different lineages. We investigate the
developmental expression and function of miRNAs in the direct-developing hemichordate Saccoglossus kowalevskii.
Hemichordates and vertebrates share a common ancestor and many developmental signaling pathways, making it an ideal model
for uncovering how ancestral miRNAs may have contributed to evolution of development in deuterostome lineage. Small RNA
sequencing shows that Saccoglossus miRNAs are dynamically expressed throughout development, suggesting potential roles in a
number of developmental processes. Target predictions are being combined with functional perturbations to reveal developmental
roles for a number of miRNAs. An initial functional screen has revealed roles for neural miRNAs and confirmed a conserved role
for miR-1 in muscle development. Targets and functions of both conserved and non-conserved Saccoglossus miRNAs will be
compared with their homologs and functional counterparts in vertebrates.
Room Assignment: R-108
William Green
MBL Association:
Principal Investigator
Institution Contact Information:
University of Chicago
Neurobiology, Pharmacology & Physiology
947 East 58th Street
Chicago IL 60637
Email:
wgreen@uchicago.edu
Dates at MBL:
6/22/2014 - 8/9/2014
Research Title:
Super-resolution to analyze post-synaptic density scaffolds and tracking of AMPA-type and NMDA-type
glutamate receptors
Research
Description:
Synapses are the basic cellular units modified during learning and memory formation. A major barrier to characterizing synaptic
function and plasticity is that the synaptic structures of most interest—presynaptic vesicles, presynaptic active zones, the synaptic
cleft, postsynaptic densities (PSDs) and postsynaptic receptors—are mostly below the diffraction-limit of light. While some of these
structures are resolvable using electron microscopy (EM), EM cannot assay structures in live tissue or real time, and has
significant problems identifying specific molecules and with sample preparation artifacts. These limitations prevent observing how
synapses change during plasticity events that underlie memory formation, such as long-term potentiation (LTP) and depression
(LTD), as well as synaptic changes occurring during neurodegenerative diseases, such as Alzheimer’s Disease or during
neurodevelopmental diseases, such as Autism Spectrum Disorders. In this proposal we apply different super-resolution
fluorescence microscopy techniques to live excitatory CNS synapses that improve imaging accuracy and resolution to near that of
EM. More specifically, we will test our hypothesis that AMPA-type and NMDA-type glutamate receptors (AMPARs/NMDARs) have
unique distributions and mobility within PSDs because of their differential interactions with the scaffold proteins, PSD-95 and
SAP97.
Room Assignment: R-322
18
Alexander Groh
MBL Association:
PI-Grass Fellow
Institution Contact Information:
Technical University of Munich
Institute of Neuroscience
Biedersteinerstr. 29
Munich Bavaria 80802
Email:
alexander.groh@gmail.com
Dates at MBL:
5/26/2014 - 8/30/2014
Research Title:
Research
Description:
The role of electrical synaptic transmission in synchronizing thalamic activity
Room Assignment: R-201,223
The role of electrical synaptic transmission in synchronizing thalamic activity
The role of synchronized activity in the thalamus is crucial to understanding key brain functions such as perception or attentional
shifts. How such synchronized activity is influenced by electrical coupling (EC) in thalamic circuits has been only sparsely
addressed. Recent evidence for thalamic EC in the intact brain from my own recordings triggered my interest in addressing this
issue in a simplified system (brain slices). This allows the application of both electrophysiological and imaging techniques for a
systematic assessment of thalamic EC and a putative function of EC in synchronizing thalamo-cortical activity.
Jeffrey Gross
MBL Association:
Principal Investigator
Institution Contact Information:
University of Texas at Austin
Molecular Biosciences
Box C1000
1 University Station
Austin TX 78712
Email:
jmgross@mail.utexas.edu
Dates at MBL:
6/1/2014 - 9/15/2014
Research Title:
Research
Description:
Molecular analysis of eye development in squid
Room Assignment: R-211
The Cephalopod eye, along with the vertebrate single-chambered eye and the Drosophila compound eye, are three examples of
high-acuity, complex, image-forming eyes. This is a rare organ and ability, found only in the Vertebrata, Cephalopoda, and a few
subphyla of the Arthropoda. While much is known about the development and morphogenesis of the vertebrate and Drosophila
eye, almost nothing is know about the development of the Cephalopod eye. We have focused on building the squid, Loligo pealeii
as a model Cephalopod for eye research, and we have generated a substantial amount of molecular and genomic data to provide
a strong foundation for further studies. Experiments this summer will continue our work to characterize eye development in Loligo
pealeii. We will quantify the spatial and temporal aspects of progenitor proliferation, determine whether apoptosis shapes the
developing eye, generate a fate-map of the stage 18 eye placode, and identify cell-type specific markers for use in the eye to
better understand differentiation events.
19
Marc Hammarlund
MBL Association:
PI-MBL Research Awardee
Institution Contact Information:
Yale University
Genetics and CNNR
295 Congress Ave
BCMM 436E
New Haven CT 06510
Email:
marc.hammarlund@yale.edu
Dates at MBL:
6/29/2014 - 8/30/2014
Research Title:
Research
Description:
Mechanism of neuronal circuit homeostasis
Room Assignment: L-341
I am studying the undead cells of the ced-3 mutant in C. elegans. ced-3 encodes a caspase critical for programmed cell death,
and ced-3 mutants have 131 extra “undead” cells that are normally destined to die (Ellis 1986). Most of these undead cells are
thought to differentiate into neurons, and previous studies indicate that at least one of these undead neurons can integrate into
functional neuronal circuits (Avery & Horvitz 1987). However, undead cells have not been studied due to a lack of tools. I am
building tools to label, isolate, activate, or kill these undead cells. These tools will allow me to investigate the unique biology of the
undead cells, and to analyze how they are incorporated into existing physical structures and neuronal circuits.
Avram Hershko
MBL Association:
Principal Investigator
Institution Contact Information:
Technion- Israel Institute of Technology, Fac. of Medicine
Biochemisry
1 Efron St.
Haifa Israel 31096
Email:
hershko@tx.technion.ac.il
Dates at MBL:
7/7/2014 - 8/28/2014
Research Title:
Research
Description:
Control of cell division by ubiquitin-mediated protein degradation
Room Assignment: R-311
Programmed ubiquitin-mediated degradation of cell cycle regulatory proteins is essential for the control of cell division. I wish to
gain insight into the underlying molecular mechanisms.
20
Yeowool Huh
MBL Association:
PI-Grass Fellow
Institution Contact Information:
Korea Institution of Science and Technology
Neuroscience
L7313, KIST
Hawolgok-dong Seonbuk-gu
Seoul NA 136791
Email:
lum00@kist.re.kr
Dates at MBL:
5/26/2014 - 8/30/2014
Research Title:
Research
Description:
Gating of pain pathways by activity-dependent recruitment of inhibitory circuits
Room Assignment: R-201,223
The cortex and the thalamus are intricately coordinated to modulate sensory information. All sensory inputs, except for olfaction,
are passed through the thalamus and the characteristic ability of thalamic neurons to switch between tonic and firing has been
suggested to play a role in sensory modulation before transmitting sensory signals to the cortex. Of the two firing modes, altered
burst firing has been repeatedly implicated with pathological pain condition, which suggests that maintaining a certain form of
thalamic bursts could be crucial for controlling pain. Moreover, results of a thalamic stimulation study have shown that electrical
stimulations mimicking specific elements of the naturally occurring bursts were important in producing an anti-nociceptive effect. It
also suggested that the anti-nociceptive effect may occur via the recruitment of cortical inhibitory interneurons, but actual
activation of cortical inhibitory neurons or the type of interneurons recruited by specific burst stimulations has not been directly
demonstrated. Therefore, the current study proposes to directly investigate the relationship between cortical inhibitory interneuron
activity and thalamic burst stimulations utilizing the slice patch recording technique. Results of the proposed study is expected to
determine part of the cortical network involved in the action mechanism of nociceptive signal modulation occurring by thalamic
burst stimulations and may possibly offer a therapeutic target for pain control.
Elizabeth Jonas
MBL Association:
Principal Investigator
Institution Contact Information:
Yale University
Internal Medicine
PO Box 208020
New Haven CT 06520
Email:
elizabeth.jonas@yale.edu
Dates at MBL:
6/15/2014 - 9/15/2014
Research Title:
Research
Description:
Mitochondrial ion channels in synaptic transmission
Room Assignment: R-213
Mitochondria are necessary for providing energy for cell metabolism. Synaptic transmission is potentiated over the long term
during learning. Changes in neuronal metabolism and mitochondrial morphology or positioning may be necessary for long term
changes in synaptic transmission that underlie learning. These events could become disrupted in neurodegenerative diseases
such as Alzheimer's and Parkinson's disease or during and after stroke.
21
Erik Jorgensen
MBL Association:
PI-Lillie Awardee
Institution Contact Information:
University of Utah/HHMI
Biology
257 S 1400 E, Room 201
Salt Lake City UT 84112-0840
Email:
jorgensen@biology.utah.edu
Dates at MBL:
6/27/2014 - 7/27/2014
Research Title:
Research
Description:
Ultrafast endocytosis during LTD
Room Assignment: R-310
Activity-dependent change in the number of AMPA receptors in the postsynaptic density is thought to underlie memory formation.
Long-term depression is caused by the reduction of AMPA receptors on the surface. Through a collaboration with the Rosenmund
lab, we will study endocytosis of AMPA receptors using state-of-the-art methods in electrophysiology and electron microscopy.
The Rosenmund lab has developed protocols to characterize long-term depression in hippocampal cell cultures. ‘Flash-andfreeze’ electron microscopy and ‘nano-fEM’ allow the direct observation of membrane or protein dynamics with ~5 millisecond
temporal resolution and ~10 nanometer spatial resolution. The synergy of these technologies should allow us to correlate
physiology with morphology in a way that has not been previously possible.
Leonard Kaczmarek
MBL Association:
Principal Investigator
Institution Contact Information:
Yale University
Pharmacology
333 Cedar St.
PO Box 208066
New Haven Connecticut CT 06520
Email:
leonard.kaczmarek@yale.edu
Dates at MBL:
6/18/2014 - 9/15/2014
Research Title:
Research
Description:
Regulation of synaptic transmission by BCL proteins
Room Assignment: R-213
A key problem in neurobiology is to understand how the properties of neurons become modified so as to produce prolonged
changes in the behavior of an animal or human. To understand the mechanisms of prolonged changes in neuronal excitability, our
lab has isolated the genes for many ion channels that determine the particular way that a neuron responds to its external inputs.
The systems of neurons that we have used to investigate these questions include neurons of squid and the sea hare Aplysia, as
well as mammalian neurons. At the MBL we investigate the factors that control opening of ion channels in mitochondria leading to
changes in synaptic strength, as well as influencing the death or survival of a neuron in response to changes in its environment.
22
Rolf Karlstrom
MBL Association:
PI-Grass Imaging Awardee
Institution Contact Information:
University of Massachusetts
Biology
611 N Pleasant
Dept. of Biology University of Massachus
Amherst MA 01003
Email:
karlstrom@bio.umass.edu
Dates at MBL:
7/7/2014 - 8/23/2014
Research Title:
Research
Description:
Fate Mapping the Zebrafish Pituitary Gland
Room Assignment: R-421
The vertebrate pituitary gland and lens originate as cranial placodes at the anterior margin of the developing nervous system. Our
work suggests that the zebrafish pituitary placode is patterned very early in development, with distinct regions of the
adenohypophysis being established soon after induction of the pituitary placode at the anterior margin of the neural keel. This
summer I will be examining the morphogenetic movements that give rise to the pituitary gland and lens. I will use 4-dimensional
cell tracking methods to create a fate map of the anterior margin of the CNS, the region that gives rise to pituitary and lens tissue.
These studies will contribute to our understanding of the origins of the pituitary and lens. Given the conservation in pituitary
structure and function across vertebrate species, this work in zebrafish can inform studies in mammals and provide insight into the
mechanisms underlying human birth defects that affect the pituitary and ventral forebrain.
U. Benjamin Kaupp
MBL Association:
Principal Investigator
Institution Contact Information:
Research Center caesar
Molekulare Neurosensorik
Ludwig-Erhard-Allee 2
Bonn NRW 53175
Email:
u.b.kaupp@caesar.de
Dates at MBL:
7/1/2014 - 8/30/2014
Research Title:
Research
Description:
Chemotactic signaling pathway in sea urchin sperm
Room Assignment: R-312
23
James Kittelberger
MBL Association:
PI-Grass Lab Associate Director
Institution Contact Information:
Gettysburg College
Biology
300 N. Washington St.
Gettysburg PA 17325
Email:
mkittelb@gettysburg.edu
Dates at MBL:
5/20/2014 - 8/31/2014
Research Title:
Research
Description:
Catecholamine modulation of vocal circuit function in a teleost fish.
Room Assignment: R-201,223
My primary research goal is to explore how the catecholamine neurotransmitters modulate social vocal behavior in a species of
teleost fish, the plainfin midshipman. Recent experiments in my lab have demonstrated that catecholamines are expressed in the
vocal circuit of the midshipman brain; that their expression is sexually dimorphic (Goebrecht et al., 2014); that injections of
dopamine (DA) to one portion of the vocal circuit (the periaqueductal gray) rapidly and reversibly inhibit vocal production; and that
this dopamine-induced vocal inhibition is mediated both by D1- and D2-type dopamine receptors. One possibility, consistent with
DA’s well-established role as a mediator of appetitive rewards across vertebrates, is that DA release is triggered by the arrival of a
gravid female in the nest of a courting male. In this situation, males cease courtship vocalizations and initiate copulatory behavior.
The next steps in testing this hypothesis are to determine a) specifically which populations of catecholamine neurons innervate
specific portions of the vocal circuit, and b) the behavioral contexts (social, vocal, auditory, etc) in which catecholamine neurons
are active. The first goal will be addressed by injecting neuronal tracers into the midshipman vocal circuit, coupled with
immunohistochemical labeling for the catecholamine synthesizing enzyme tyrosine hydroxylase (TH). The second goal will be
achieved using immediate-early genes to map patterns of activity in midshipman brains in vocally-active and non vocally-active
fish, with a particular emphasis on the activity of catecholamine neurons, as identified by TH-labeling.
David Kovar
MBL Association:
PI-MBL Research Awardee
Institution Contact Information:
The University of Chicago
Molecular Genetics and Cell Biology
920 East 58th St
Chicago IL 60637
Email:
drkovar@uchicago.edu
Dates at MBL:
7/13/2014 - 8/2/2014
Research Title:
Research
Description:
Homeostatsis F-actin networks compete for actin monomers
Room Assignment: L-104,105
Cells utilize multiple actin assembly factors such as Arp2/3 complex and formin to assemble diverse F-actin networks within a
common cytoplasm for diverse processes. These F-actin networks are usually investigated separately. However, we recently
discovered that competition between Arp2/3 complex and formin for a limited pool of actin monomers helps regulate the size and
density of F-actin networks in fission yeast. To begin to understand the extent to which the actin cytoskeleton in diverse cell types
are similarly regulated, we plan to assess the consequences on F-actin network density upon treating different cells with small
molecular inhibitors specific for particular actin assembly factors (CK-666: Arp2/3 complex; SMIFH2: Formin).
24
Wei-Lih Lee
MBL Association:
PI-MBL Research Awardee
Institution Contact Information:
UMass Amherst
Biology Department
221 Morrill South
611 North Pleasant St
Amherst MA 01003
Email:
wlee@bio.umass.edu
Dates at MBL:
6/23/2014 - 8/15/2014
Research Title:
Research
Description:
Mechanism of ER attachment and polarization during nuclear migration in S.cerevisiae
Room Assignment: L-104,105
How organelles polarize and segregate during asymmetric cell division is not known. We have uncovered a role for Num1, the
membrane attachment factor for dynein, in asymmetric polarization of endoplasmic reticulum (ER) during nuclear migration in
budding yeast S. cerevisiae. In cells lacking Num1, asymmetric bud cell-directed fenestration of nuclear envelope-associated ER
(nER) is lost. This summer at MBL, using rapid multi-color fluorescence imaging techniques, we will identify and characterize the
steps involved in the initiation of ER polarization. This study will provide mechanistic insights into the role of Num1 and dynein in
ER segregation.
Rodolfo Llinas
MBL Association:
Principal Investigator
Institution Contact Information:
New York University School of Medicine
Physiology & Neuroscience
550 First Avenue
4th Floor, Rm. 442
New York City NY 10016
Email:
rodolfo.llinas@nyumc.org
Dates at MBL:
5/1/2014 - 10/31/2014
Research Title:
Research
Description:
Transmitter release in the squid giant synapse
Room Assignment: R-207,208,209
Experiments will be performed to determine the mechanism by which synaptic transmitter is released into the giant synapse of the
squid stellate ganglia.
25
Artur Llobet
MBL Association:
PI-MBL Research Awardee
Institution Contact Information:
Bellvitge Biomedical Research Institute / Univ. Barcelona
Neuroscience
Feixa Llarga s/n
Pavello de Govern-Lab. 4112
L'Hospitalet de Llobregat Catalonia/Ba 08907
rcelona
Email:
allobet@ub.edu
Dates at MBL:
6/16/2014 - 8/4/2014
Research Title:
Research
Description:
Rewiring of sensory inputs in the Xenopus brain
Room Assignment: Lb NXR G12
Some amphibians are capable to recover the functionality of their sensory inputs after suffering traumatic injuries. Following a
lesion, their neuronal circuits can be repaired and synaptic connections restored to normal function. Using transgenic Xenopus
tadpoles, I aim to understand how synaptic connections established by olfactory sensory neurons recover the capacity to transmit
information after injury. My experimental methods are based in morphological approaches and measurements of intracellular
calcium changes in vivo. Results will shed light on mechanisms controlling formation, maturation and elimination of synapses
during rewiring of neuronal networks.
Christopher Lowe
MBL Association:
Principal Investigator
Institution Contact Information:
Hopkins Marine Station of Stanford University
Biology
120 Oceanview Blvd.
Pacific Grove CA 93950
Email:
clowe@stanford.edu
Dates at MBL:
9/1/2014 - 9/30/2014
Research Title:
Research
Description:
Insights from hemichordates
Room Assignment:
Utilization of the early development in hemichordates as a system to gain a better understanding of aspects of axis formation in
ancestral lineages leading to modern chordates.
26
Jane Maienschein
MBL Association:
Principal Investigator
Institution Contact Information:
Center for Biology and Society
School of Life Sciences 874501
Arizona State University
Tempe Arizona 85287-4501
Email:
maienschein@asu.edu
Dates at MBL:
5/17/2014 - 9/16/2014
Research Title:
Research
Description:
MBL History Digital Archives Project
Room Assignment: Library, L-228
Based in a digital repository (hpsrepository.asu.edu), we have developed a set of digital and computational projects. These
include the online Embryo Project Encyclopedia (embryo.asu.edu) and MBL Archives Project (history.archives.mbl.edu). In 2014,
the lab will focus on digitizing historically important scientific collections such as the Viktor Hamburger and the J.P. Trinkaus
Collections and other materials related to ecosystems, adding metadata, and developing interpretive exhibits. We welcome
contributions of material, stories, or interpretations. This work is funded by several grants from the NSF and Arizona State
University.
Robert Malchow
MBL Association:
Principal Investigator
Institution Contact Information:
University of Illinois at Chicago
M/C 067
840 West Taylor Street
Chicago IL 60607
Email:
paulmalc@uic.edu
Dates at MBL:
6/17/2014 - 8/23/2014
Research Title:
Research
Description:
Measurement of H+ and zinc transport; release from retinal neurons and glial cells
Room Assignment: MRC-306
H+ has been suggested to play a key neuromodulatory role in the shaping of visual signals. We plan to examine the regulation of
H+ transport from several types of retinal neurons and support cells and to examine the effects of H+ on voltage gated
conductances and various transport mechanisms present in these cells. We will examine these effects on isolated cells
maintained in primary culture and in cultured retinal slices. The results from these experiments are expected to significantly clarify
the role that H+ plays in retinal processing.
27
Reyna Martinez-De Luna
MBL Association:
PI-Grass Fellow
Institution Contact Information:
SUNY Upstate Medical University
Ophthalmology
5325 Weiskotten Hall Addition
117 Monroe St.
Syracuse New York 13210
Email:
martiner@upstate.edu
Dates at MBL:
5/26/2014 - 8/30/2014
Research Title:
Research
Description:
The role of intermediate filament proteins in neural degeneration and regeneration
Room Assignment: R-201,223
The expression of intermediate filament proteins (IFPs) in glial cells has been implicated in the inability of some vertebrates to
regenerate the central nervous system. Taking advantage of the ability of Xenopus laevis to regenerate the retina, I will use the
XOPNTR transgenic line, a model of rod degeneration and regeneration, to determine the role of IFPs in these two processes.
Specifically, I will investigate if the IFPs glial fibrillary acidic protein (GFAP) and Vimentin are important regulators of retinal
degeneration and the ability of the Xenopus retina to regenerate. The results of this study will determine if upregulation of GFAP
and Vimentin IFPs is required for Müller cell reactivity, retinal degeneration and restricting the regenerative capacity of the retina.
Rebecca Mease
MBL Association:
PI-Grass Fellow
Institution Contact Information:
Technical University of Munich
Institute of Neuroscience
Biedersteiner Str. 29
Munich Bavaria 80802
Email:
beckin@gmail.com
Dates at MBL:
5/26/2014 - 8/30/2014
Research Title:
Research
Description:
A conductance-based model of higher-order thalamic encoding of cortical feedback
Room Assignment: R-201,223
Corticothalamic feedback projections in the mammalian brain exert strong control over single thalamic neurons, thereby shaping
how information propagates in thalamocortical circuits implicated in sensation and global brain states. In the rodent whisker
system, one such pathway is cortical layer 5B (L5B) to posterior medial (POm) thalamus, which can drive spiking in thalamic
target neurons and serves to integrate cortical activity with incoming sensory information. Due to the combination of pronounced
synaptic depression and intrinsic POm bursting, spike transfer from L5B to POm is highly nonlinear and dependent on past
activity. I propose to model this pathway by fitting a detailed model of POm neurons and L5B depressing synapses. The resulting
model will be used to investigate thalamic encoding of low- and high- frequency information present in in vivo L5B spike trains.
28
Allen Mensinger
MBL Association:
Principal Investigator
Institution Contact Information:
University of Minnesotat Duluth
Biology
1035 Kirby Dr
Duluth MN 55812-3003
Email:
amensing@d.umn.edu
Dates at MBL:
6/15/2014 - 8/25/2014
Research Title:
Research
Description:
Multisensory integration in toadfish
Room Assignment: MRC-306
Recent experiments have demonstrated that the toadfish lateral line is sensitive to sound as well as water vibrations and that the
utricle is sensitive to sound and linear movements. Our goal is to determine how both these systems integrate multimodal sensory
integration
Andrew Miller
MBL Association:
Principal Investigator
Institution Contact Information:
HKUST
Division of Life Science
Clear Water Bay
Kowloon
Hong Kong New
HK SAR
Territories
Email:
almiller@ust.hk
Dates at MBL:
6/1/2014 - 9/30/2014
Research Title:
Research
Description:
Role of calcium signaling during embryonic development
Room Assignment: R-220
We study the role played by calcium signaling in the orchestration of embryonic development using a number of vertebrate and
invertebrate model systems.
29
Timothy Mitchison
MBL Association:
PI-MBL Research Awardee
Institution Contact Information:
Harvard Medical School
Systems Biology WA 536
200 Longwood Av
Boston MA MA 02115
Email:
timothy_mitchison@hms.harvard.edu
Dates at MBL:
6/22/2014 - 8/31/2014
Research Title:
Research
Description:
Limiting components for mitotic spindle assembly
Room Assignment: R-205,215
Early embryos are physically organized by asters; large, radial arrays of microtubules that grow out from centrosomes. Asters are
responsible for centering the centrosome and nucleus in the egg or blastomere, and the cleavage furrows are positioned at the
interaction plane between two asters. We will study how aster grow and interact in Xenopus eggs. These are huge cells (1.2mm in
diameter), which emphasize the scaling challenges inherent in organizing a cell on the micron scale using molecular processes on
the nm scale. Working at MBL 2009-12 we developed methods for reconstituting large embryo asters and the interactions
between them in extracts from Xenopus eggs. These methods allow biochemical manipulation and high resolution live imaging. In
2013 we will address two problems, how do aster grow out from centrosomes, and how do they interact to form planes that
subdivide the embryo and signal cleavage furrow position? We have evidence that asters grow by a process where microtubules
nucleate from the sides of pre-existing microtubules. In summer 2013 we will build mathematical models of this process and refine
them using microscopy data. We will also try to dissect the molecular mechanism by which microtubules nucleate. We will image
and quantify microtubule dynamics in aster-aster interaction zones, and determine the role in interaction zone formation of several
new molecular players, including three motor proteins, Kif4, Kif20A and Kif23. Our project will increase conceptual understanding
of how early embryos self-organize in space and time. It may also provide clues to cell organization mechanisms in other large
cells such including neurons.
30
Gerardo Morfini
MBL Association:
Principal Investigator
Institution Contact Information:
University of Illinois
Anatomy and Cell biology
808 S. Woods St
CME Rm#568
Chicago IL 60612
Email:
gmorfini@uic.edu
Dates at MBL:
7/1/2014 - 9/1/2014
Research Title:
Research
Description:
Molecular mechanisms for fast axonal transport in health and disease.
Room Assignment: R-108,109
The exquisitely organized distribution of cellular components within discrete subcellular compartments underlies the unique ability
of neurons to receive, process, and transmit information. Precise targeting and delivery of these components in mature neurons
largely depends upon axonal transport (AT), a cellular process largely mediated by the molecular motor proteins conventional
kinesin and cytoplasmic dynein (CDyn). An illumination of molecular mechanisms regulating AT is critical for a comprehensive
understanding of neuronal function. In this context, our recent studies identified specific protein kinases that regulate AT by
phosphorylating specific motor proteins subunits.
Strong genetic evidence in recent years demonstrated that human neurodegenerative diseases can result from alterations in AT.
Accordingly, alterations in protein phosphorylation and kinase activity have been widely documented in several neurological
conditions including Alzheimer’s, Huntington’s, and Parkinson’s diseases. This observation led us to hypothesize that alterations in
AT associated with abnormal kinase activity represent a critical pathogenic event in these diseases. Providing support to this
hypothesis, results from our research showed that pathogenic proteins associated with familiar forms of neurodegenerative
diseases differentially affect AT through various mechanisms involving selective activation of kinases and abnormal
phosphorylation of molecular motors.
The goals of our research program are: 1) to elucidate normal regulatory mechanisms of AT in neuronal cells; and 2) to determine
how alterations in AT contribute to the pathogenesis of human neurodegenerative conditions.
Adult-onset neurodegenerative diseases represent one of the most difficult and puzzling disorders faced by modern medicine. The
clinical relevance of our research relates to an understanding of molecular mechanisms underlying these diseases. An illumination
of such mechanisms will provide a conceptual framework for the development of novel therapeutic strategies.
Tetsuya Nakamura
MBL Association:
Principal Investigator
Institution Contact Information:
The University of Chicago
Organismal Biology and Anatomy
1012Ellis ave. 57 Street
Culver Hall 106
Chicago IL 60637
Email:
tetsuya@uchicago.edu
Dates at MBL:
6/20/2014 - 8/31/2014
Research Title:
Deeply conserved hox collinearity autonomously produces diversified fin patterns and evolution
depending on appendage size
Research
Description:
During the transition from fish fins into tetrapod limbs, appendage morphology shows diversified skeletal patterns as seen in a
number of fossils and extant animals. Examples of this disparity can be seen in the anteriorly elongated large cartilage of some
cartilaginous fish fins, as well as the autopod region which is exclusive to limbed animals. However, the molecular mechanisms
that produce such diversity and allow evolution from fins into limbs are still unknown. I will investigate how appendage shape is
controlled by studying the genetic mechanisms of fin development in cartilaginous fish and comparing these molecular players
with those of other animals.
Room Assignment: MRC-306
31
John Oakey
MBL Association:
Principal Investigator
Institution Contact Information:
University of Wyoming
Department of Chemical and Petroleum Engineering
1000 E University Ave
Laramie WY 82071
Email:
joakey@uwyo.edu
Dates at MBL:
6/15/2014 - 8/15/2014
Research Title:
Research
Description:
Manipulating Xenopus Extract with Microfluidics to Elucidate Scaling Mechanisms
Room Assignment: R-206
The size, shape, and overall morphology of a given intracellular organelle is often critical to its function. The nucleus and mitotic
spindle are particular examples of this rule, but how the sizes of the mitotic spindle and interphase nucleus are regulated within a
cell remains largely unknown. This gap in knowledge prevents us from understanding the functional significance of organelle size
control, particularly in the context of various cancers in which the scaling relationship between organelle and cell size has gone
awry. We have developed a microfluidic platform for encapsulating cell-free extracts, allowing us to address previously
intractable questions regarding organelle scaling. The long-term goal of this work is the identification of organelle size regulation
mechanisms in order to better understand how organelle size and morphology impact cell function. Specifically, we will address
the question of how physical constraints imposed by cell-size impact the size, shape, and function of the mitotic spindle and its
machinery.
Harish C Pant
MBL Association:
Principal Investigator
Institution Contact Information:
NINDS/ NIH
LNC
Bldg 49, Room 2A-28
Bethesda MD 20892
Email:
panth@ninds.nih.gov
Dates at MBL:
6/15/2014 - 9/3/2014
Research Title:
Research
Description:
Topographic Regulation of cytoskeletal protein phosphorylation in the squid giant fiber system
Room Assignment: R-326
We are interested in the factors regulating the synthesis, assembly,organization and function of cytoskeletal proteins in nerve
cells. We find that these proteins, though synthesized in the nerve cell body, are only phosphorylated and assembled in the axon.
Phosphorylation of these proteins changes their stability, organization and function. The question is why and how is this
compartmentalization regulated? The giant fiber system of the squid possesses giant nerve cells which can be easily separated
into axon and cell body and studied biochemically. Several enzymes, e.g., kinases and phosphatases, are principally involved and
we are studying their relative activities, mutual interactions, localizations and response to various signaling systems. In this way
we hope to understand the metabolic network that determines the organization and function of these proteins in the nerve cell in
normal and in neurodegenerative disorders in which the process is deregulated.
32
Roy Parker
MBL Association:
Principal Investigator
Institution Contact Information:
Univ of Colorado
Chemistry and Biochemistry
JSC Biotech Building
596 UCB
Boulder CO 80309
Email:
roy.parker@colorado.edu
Dates at MBL:
6/16/2014 - 8/1/2014
Research Title:
Research
Description:
Control of mRNA localization, translation and degradation
Room Assignment: R-319,320,321
The Parker lab studies the control of mRNA localization, translation and degradation in eukaryotic cells. This includes the analysis
of the mRNP assemblies referred to as stress granules and P-bodies, which are conserved cytoplasmic aggregates of nontranslating mRNPs implicated in the regulation of mRNA translation and decay, and are related to RNP granules in embryos,
neurons and in some neurodegenerative diseases. Projects in the lab include reconstitution of mRNP granules, determining their
modes of assembly and disassembly, and their biological functions.
Alberto Pereda
MBL Association:
PI- Grass Lab Director
Institution Contact Information:
Albert Einstein College of Medicine
Neuroscience
1300 Morris Park Ave
Bronx New York 10461
Email:
alberto.pereda@einstein.yu.edu
Dates at MBL:
5/23/2014 - 9/1/2014
Research Title:
Research
Description:
Regulated trafficking of gap junction channels at electrical synapses.
Room Assignment: R-201,223
The goal of my laboratory is to understand the complexity and regulatory mechanisms underlying gap junction mediated electrical
transmission. We investigate these properties at auditory afferents forming as mixed (electrical and chemical) synaptic contacts on
the goldfish Mauthner cell. Because of their unique experimental accessibility, these terminals are ideally suited for studies of
electrical transmission. The strength of these electrical synapses is under the fine regulatory control of neuronal activity. My
proposal deals on understanding the molecular mechanisms underlying the bi-directional control of junctional conductance at
these terminals by focusing in the role of regulated trafficking of gap junction channels. Thus, my research addresses the novel
concept that the strength of electrical synapses is achieved by dynamically regulating the trafficking of gap junction channels.
33
Lawrence Rome
MBL Association:
Principal Investigator
Institution Contact Information:
University of Pennsylvania
Biology Department
Leidy Labs
Philadelphia PA 19104
Email:
lrome@sas.upenn.edu
Dates at MBL:
6/1/2014 - 8/31/2014
Research Title:
Research
Description:
Calcium movements in superfast muscle of toadfish
Room Assignment: R-313
I study how muscles are designed to perform different activities particularly superfast muscles found in toadfish, rattlesnakes and
birds.
Eduardo Rosa-Molinar
MBL Association:
PI-Grass Imaging Awardee
Institution Contact Information:
University of Puerto Rico-Rio Piedras
Biological Imaging Group
P.O. Box 21809 UPR Station
San Juan Puerto Rico 00931
Email:
ed@hpcf.upr.edu
Dates at MBL:
6/28/2014 - 8/3/2014
Research Title:
Connexin composition of gap junction hemiplaques at mixed synapses between identifiable dye-coupled
spinal neurons revealed by super-resolution fluorescence imaging
Research
Description:
Electrical synapses, hereafter gap junctions, are thought to be central to integrating and regulating basic cell processes and to
synchronizing fast motor behavior, but most gap junction studies have focused on gap junctions’ role in development, perhaps
because they are generally thought not to persist into adulthood. Based on prior studies of gap junctions and on our on-going
studies of our “reference species”, the adult the Western Mosquitofish, Gambusia affinis (hereafter Mosquitofish), we propose to
determine the type, number, and extent of synapses in the neural circuitry responsible for an extremely rapid coital movement (20
-50 ms) of the sexually dimorphic internal fertilizing adult male Mosquitofish. During my summer, 2014 tenure at the Marine
Biological Laboratory, I will develop a novel imaging workflow for whole-mount preparations that integrates direct STochastic
Optical Reconstruction Microscopy (dSTORM), grid-mapped freeze-fracture-matched-double-replica-immunogold-labeling, and
serial block-face imaging using a gallium focused ion beam low voltage field emission scanning electron microscopy. The imaging
workflow will enhance the ability to: 1) accurately locate gap junctions at mixed synapses; 2) unambiguously identify one or two
constituent synaptic proteins found at mixed synapses; and 3) determine membrane “sidedness.” This imaging workflow has
tremendous potential to provide new views and insights into the structure and molecular organization of gap junctions at mixed
synapses.
Room Assignment: L-208
34
Robert Rose
MBL Association:
PI-MBL Research Awardee
Institution Contact Information:
North Carolina State University
Biochemistry
128 Polk Hall
Raleign NC 27695-7622
Email:
bob_rose@ncsu.edu
Dates at MBL:
6/18/2014 - 7/31/2014
Research Title:
Research
Description:
Transcriptional regulation of pancreas development
Room Assignment: R-401,423
My lab at North Carolina State University studies the interactions among transcription factors that regulate pancreas development,
using protein crystallography and cell culture assays. This summer I will be working with Marko Horb at the National Xenopus
Resource to characterize the role of a transcription factor complex, between the factors Pdx1 and Pbx1. Pdx1 is a master
regulator of pancreas development and we propose that its interaction with Pbx1 regulates proliferation, a critical aspect of
development. We plan to up-regulate and down-regulate Pdx1 and Pbx1 at various stages of Xenopus development and measure
expansion of pancreatic exocrine and endocrine cells. Since Pdx1 regulates hundreds of genes, we have generated a Pdx1-Pbx1
fusion to restrict activity to sites regulated by the complex.
Michael Rosen
MBL Association:
Principal Investigator
Institution Contact Information:
UT Southwestern Medical Center
Biophysics
5323 Harry Hines Blvd.
Dallas Texas 75390
Email:
michael.rosen@utsouthwestern.edu
Dates at MBL:
6/16/2014 - 6/28/2014
Research Title:
Research
Description:
Partitioning and polymerization--mechanisms for regulating protein activity
Room Assignment: R-319,320,322
Research at the Summer Institute is directed toward understanding how micron sized cellular structures at membranes and in the
cytosol are generated through the polymerization and phase separation of macromolecules and lipids.
35
Joshua Rosenthal
MBL Association:
PI-MBL Research Awardee
Institution Contact Information:
Universidad de Puerto Rico/Recinto de Ciencias Medicas
Institute of Neurobiology
201 Blvd. del Valle
San Juan Puerto Rico 00901
Email:
joshua.rosenthal@upr.edu
Dates at MBL:
6/20/2014 - 8/10/2014
Research Title:
Research
Description:
Development of a resource for transcripomes from non-model organisms
Room Assignment: R-219
Advancements in next-generation DNA sequencing technologies allow us to generate large sequence databases from any
organism at a low cost. Our group, in close collaboration with Dr. Titus Brown, aims to create an open access resource for
cephalopod transcriptomes and genomes, and also a freely accessible set of tutorials so that others can generate similar
resources. Accordingly, last summer we generated five different transcriptomes from the nervous system of Loligo pealei (http:
//ivory.idyll.org/blog/2014-loligo-transcriptome-data.html) and also created the “ Eel Pond mRNAseq tutorials (https://khmerprotocols.readthedocs.org/en/latest/mrnaseq/index.html). This summer we plan to significantly expand the number of Loligo
transcriptomes and to set up a server to host these resources at the MBL.
Benjamin Rost
MBL Association:
PI-Grass Fellow
Institution Contact Information:
German Center for Neurodegenerative Diseases (DZNE)
Charité - CCO, Schmitz group
Charitéplatz 1
intern Virchowweg 6
Berlin Berlin 10117
Email:
benjamin.rost@charite.de
Dates at MBL:
5/23/2014 - 8/30/2014
Research Title:
Research
Description:
Physiological correlates of fast postsynaptic endocytosis
Room Assignment: R-201,223
In hippocampal neurons, long-term depression (LTD) can be induced by prolonged low-frequency stimulation and activation of
NMDARs, or activation of metabotropic glutamate receptors. LTD may also be caused by presynaptic transmitter release shortly
after a postsynaptic back-propagating action potential that has unblocked synaptic NMDARs (pairing LTD). In consequence,
clathrin mediates endocytosis of AMPARs residing outside of the postsynaptic density, on a time-scale of 20 s and longer. Recent
experiments using precisely timed optogenetic stimulation and high-pressure freezing followed by electron-microscopy provided
evidence for much faster postsynaptic endocytic events. If rapid postsynaptic endocytosis removes AMPARs from the surface it
should cause synaptic depression. To test this hypothesis, I will determine the kinetics and the extent of fast AMPAR endocytosis
in living neurons. In combination with genetic or pharmacological manipulations, I will then investigate the molecular machinery
underlying this novel form of AMPAR surface regulation.
36
Paul Selvin
MBL Association:
PI-Grass Imaging Awardee
Institution Contact Information:
University of Illinois Urbana Champaign
Physics
1110 W. Green St.
Illinois Urbana 61801
Email:
Selvin@UIUC.edu
Dates at MBL:
6/15/2014 - 7/25/2014
Research Title:
Organization and Dynamics of PSD-bound Glutamate Receptors by Fluorescence Super-Resolution
Microscopy.
Research
Description:
Synapses are the basic cellular units modified during learning and memory formation. A major barrier to characterizing synaptic
function and plasticity is that many of the synaptic structures are below the visible diffraction-limit of light, or ~250 nm. We are
developing super-resolution fluorescence technique which are able to image live synapses in cultured neurons of rat hippocampi
to about 10-20 nm in x-y and about 25-40 nm in z. Furthermore, we have developed small quantum dots (~ 8 nm) as labels which
are able to get into the synaptic cleft and hence label synaptic receptors such as AMPAR and NMDAR. These are visible at the
single-molecule, have ~30x the usual brightness of fluorescent dyes, and are essentially indestructible. As a result, we can follow
the behavior of labeled single receptors with 10-100 millisecond time resolution. So far, we have found that AMPA-receptors are
surprising free to diffuse within the synaptic densities
Room Assignment: R-322
Roger Sloboda
MBL Association:
Principal Investigator
Institution Contact Information:
Dartmouth College
Biological Sciences
306 Gilman
Hanover NH 03755
Email:
rds@dartmouth.edu
Dates at MBL:
6/20/2014 - 8/30/2014
Research Title:
Research
Description:
Room Assignment: L-121
Assembly and disassembly of cilia and flagella
Cells with cilia and flagella must disassemble these organelles at each cell division in order to free the basal bodies for
participation in the assembly of the mitotic apparatus. We are focused currently on a protein methylation pathway that is activated
upon disassembly, and gather cila from marine organisms at the MBL for comparative studies.
37
Greenfield Sluder
MBL Association:
Principal Investigator
Institution Contact Information:
UMass Medical School
Cell and Developmental Biology S6-212
55 Lake Ave. North
Worcester MA 01655
Email:
greenfield.sluder@umassmed.edu
Dates at MBL:
5/1/2014 - 10/1/2014
Research Title:
Research
Description:
Investigation of the controls for centrosome duplication
Room Assignment: R-308
My research at the MBL uses sea urchin zygotes as a favorable experimental system to study the mechanisms that control
centrosome duplication (the one to only two doubling in preparation for mitosis). My work this summer will continue research
initiated last summer that focuses on the mechanisms that control the splitting of centrosomes which is a necessary event for later
centrosome duplication. I have published an interesting but unexplained phenomenon in which centrosomes split only once
during prolonged mitosis in sea urchin zygotes. This phenomenon does not fit in any simple way the current understanding of
what drives centrosome splitting. I will seek to provide insight into this phenomenon by using new small molecule inhibitors of key
enzymes (catalytic proteins) that have been implicated in centrosome splitting during other phases of the cell cycle. The goal is to
use an unusual phenomenon to provide more information on the normal mechanisms that control centrosome duplication and
prevent overduplication/reduplication of the centrosome (as is found in some cancer cells).
Yuyu Song
MBL Association:
PI-Grass Fellow
Institution Contact Information:
HHMI/Yale Medical School
Genetics
295 Congress Avenue BCMM 154
Box 9812
New Haven CT 06536-0812
Email:
yuyu.song@yale.edu
Dates at MBL:
5/26/2014 - 8/30/2014
Research Title:
Research
Description:
Synaptic Actions of misfolded ALS-associated G*%R-SOD1 protein
Room Assignment: R-201,223
Amyotrophic lateral sclerosis (ALS) is a lethal neurodegenerative disease that affects motor neurons and causes progressive
muscle weakness, atrophy and paralysis. The best studied subtype of ALS results from mutations in the SOD1 gene (e.g. G85R),
whose protein products are misfolded and may cause dysfunction of neuromuscular junctions (NMJ), loss of synapses, “dyingback” degeneration of axons, and neuronal death. Early changes in NMJ suggest an essential role of synaptic dysfunction in ALS
pathology. However, the molecular mechanism for the altered synaptic function in ALS is poorly understood, partially due to the
lack of a motor neuron system where acute effects can be studied. Using a unique model of squid giant synapse, I will start to
answer questions like whetherG85R-SOD1 directly inhibits synaptic transmission, if so, what is the underlying molecular
mechanism? Can we prevent or rescue synaptic dysfunction with chaperones? These approaches will help us understand ALS
neuropathology, as well as identify potential therapeutic targets for clinical intervention.
38
Timothy Springer
MBL Association:
Principal Investigator
Institution Contact Information:
Childrens Hospital/ Harvard Medical School
BCMP
CLSB, 3 Blackfan Circle
Boston MA 02115
Email:
springer@crystal.harvard.edu
Dates at MBL:
5/30/2014 - 9/7/2014
Research Title:
Research
Description:
Cell biology of integrins.
Room Assignment: R-305
Our structural work on integrins shows that they assume three overall conformational states. A resting low-affinity state is bent.
Another low-affinity state is extended. The high-affinity state is extended, with a swung out hybrid domain. Swing-out of 70 Å at the
knees is communicated by a rigid lever arm and alpha-helix pistoning to a 3 Å movement at the ligand binding site. This large
amplification seems important to transmit allostery through the flexible integrin lower legs. We hypothesize that tensile force
imposed by the actin cytoskeleton on the integrin beta subunit cytoplasmic domain, and resisted by ligands bound to the
extracellular matrix or on the surface of other cells, is on pathway with allosteric change to the high affinity state. In other words,
force acts as an allosteric effector, and couples ligand binding by integrins to their attachment to the actin cytoskeleton. We
propose this as a mechanism to give cells four-wheel drive; it avoids integrins spinning their wheels in regions of a cell where they
cannot grip the substrate. We have supported this hypothesis with steered molecular dynamics simulations and studies with
conformation-selective antibodies that demonstrate the relation of conformational state to affinity for ligand. At MBL we are testing
this hypothesis in cells using advanced fluorescence polarization microscopy techniques.
Sidney Tamm
MBL Association:
Principal Investigator
Institution Contact Information:
Marine Biological Laboratory
Bell Center
7 MBL Street
Woods Hole MA 02543
Email:
tamm@bu.edu
Dates at MBL:
6/15/2014 - 10/15/2014
Research Title:
Research
Description:
Regeneration and Motility of Ctenophores
Room Assignment: R-113
Regeneration of ciiiary comb plates, formation of the statolith, and locomotion and coordination of comb rows in ctenophores.
39
Mark Terasaki
MBL Association:
Principal Investigator
Institution Contact Information:
University of Connecticut Health Center
Cell Biology
263 Farmington Ave
Farmington CT 06032
Email:
terasaki@uchc.edu
Dates at MBL:
5/15/2014 - 10/31/2014
Research Title:
Three dimensional organization of the endoplasmic reticulum
Research
Description:
We recently discovered a new structural motif of the endoplasmic reticulum consisting of helical ramps that connect parallel
sheets. We will be looking for more structural features involving tubules and the connections between tubules and sheets. We
will also work on serial section immunolabeling to localize functions or structural proteins to sub-regions of the endoplasmic
reticulum.
Room Assignment: R-113
Steven Treistman
MBL Association:
Principal Investigator
Institution Contact Information:
Univ. Puerto Rico Medical Sciences
Institute of Neurobiology,
201 Blvd del Valle
San Juan PR 00901
Email:
steven.treistman@upr.edu
Dates at MBL:
6/1/2014 - 9/30/2014
Research Title:
Research
Description:
Puerto Rico Center for Environmental Neuroscience
Room Assignment: R-219
The nervous system is the interface between an organism and its environment. The Puerto Rico Center for Environmental
Neuroscience (PRCEN) will combine neuroscience (the study of the nervous system and behavior) and environmental science
(the study of local ecosystem environments) to tackle environmental issues in Puerto Ricos tropical setting. The Center will
combine neuroscientists from the Institute of Neurobiology and the Dept. of Anatomy of the UPR Medical Sciences campus and
environmental scientists from the Environmental Sciences Program and the Depts. of Biology and Chemistry of the UPR Rio
Piedras campus. The alliance will bring together cutting-edge techniques normally associated with cellular and molecular
neuroscience with expertise in local ecosystems and environmental science to create a novel field.
40
Ron Vale
MBL Association:
Principal Investigator
Institution Contact Information:
UCSF
Cellular and Molecular Pharmacology
600 16th Street UCSF
Genentech Hall n312
San Francisco CA 94158
Email:
vale@cmp.ucsf.edu
Dates at MBL:
6/15/2014 - 8/31/2014
Research Title:
Research
Description:
HCIA-Summer Institute Program
Room Assignment: R-319,320,321
Neurons are distinct from most cell types in that they are highly elongate. At one end of the neuron is the cell body (which houses
the nucleus and DNA) and at the other end resides the nerve terminal that communicates signals to other cells. In between is a
very long and thin tube called the axon (which can be a meter long in humans). The outer membrane of the axon serves as an
electrical conduit while the interior is designed to serve as a highway to transport building blocks between the cell body and the
nerve terminal. The actual tracks or roadways of this highway are called microtubules, long polymers made up of a protein called
tubulin. The goal of this summer project is to understand axonal microtubules, specifically how they are made and how they
change over time.
Shigeki Watanabe
MBL Association:
PI-Grass Fellow
Institution Contact Information:
University of Utah
Biology
257 South 1400 East
Salt Lake City UT 84112
Email:
watanabe@biology.utah.edu
Dates at MBL:
5/26/2014 - 8/20/2014
Research Title:
Research
Description:
Mechanisms of synaptic plasticity in mouse hippocampal synapses
Room Assignment: R-201,223
Use-dependent synaptic potentiation and depression are thought to underlie memory formation in the mammalian brain. One
pathway for long-term depression is mediated by calcium influx through NMDA receptors, which triggers AMPA-type glutamate
receptor endocytosis. I have developed two techniques in electron microscopy; "Flash-and-freeze" electron microscopy (EM)
combines optogenetic neuronal stimulation ("flash") with rapid, high-pressure freezing ("freeze") to capture endocytosis on a
millisecond timescale; nano-fEM combines super-resolution fluorescence microscopy with EM to visualize fluorescently tagged
proteins within their sub-cellular context. Using these techniques, I will test how AMPA receptors are endocytosed from the
surface.
41
Clare Waterman
MBL Association:
Principal Investigator
Institution Contact Information:
National Institutes of Health
Cell Biology and Physiology Center
10 Center Drive, Room 7N220
Bethesda MD 20892
Email:
watermancm@nhlbi.nih.gov
Dates at MBL:
6/10/2014 - 8/5/2014
Research Title:
Research
Description:
Integrin activation and organization by actin dynamics during cell migration.
Room Assignment: R-305, 307
The adhesion and migration of cells are critical for their normal function and are governed by the transmembrane receptors
integrins, which localize to intracellular complexes called focal adhesions. The activation of integrins allows cells to attach and
exert force on their surroundings to migrate. What controls the coordinated activation of integrins is not understood. We want to
test a model where the actin cytoskeleton binding to the cytoplasmic side of aVβ3 integrins and mechanically forces them into an
active state. Two testable predictions come out of this: aVβ3 integrins are organized in aligned clusters at focal adhesions and that
movement and organization of the actin cytoskeleton cause this. We will test these hypotheses with a combination of state-of-theart orthogonal imaging techniques, both of which will be setup at the MBL for the coming two summers. Our preliminary results
show that by using specifically designed constructs, we can study integrin alignment using fluorescence emission polarization
anisotropy microscopy, and with the novel TIRF PolScope at MBL, we can measure the actual orientation of integrins in migrating
cells. With multi-channel acquisition we will simultaneously monitor actin dynamics and focal adhesion markers. This proposed
project is a multi-lab collaboration with the Cell dynamics Laboratory at the MBL, where the participating labs each provide the
world-leading expertise required to successfully test these hypotheses. In typical “blue sky” thinking approach, this project was
conceived as part of the physiology course at the MBL. Within the timeframe of this project we expect to have fully explored the
importance of actin dynamics for integrin activation in migrating cells. Integrins are essential proteins and proven drug targets, with
several integrin-based biologicals in use to treat patients, including multiple sclerosis and Crohn's disease. A further
understanding of their activation mechanism will likely yield new avenues and approaches for future drugs.
Jim Wilhelm
MBL Association:
Principal Investigator
Institution Contact Information:
UC San Diego
Cell and Developmental Biology
9500 Gillman Drive
Pacific Hall 2123A
La Jolla CA 92093-0347
Email:
jwilhelm@ucsd.edu
Dates at MBL:
6/14/2014 - 8/16/2014
Research Title:
Research
Description:
HCIA-Summer Institute Program
Room Assignment: R-319,320,322
This summer we will be focused on in vitro reconstitution of several novel intracellular filaments that we have discovered.
42
Hao Wu
MBL Association:
Principal Investigator
Institution Contact Information:
Boston Children's Hospital and Harvard Medical School
Program in Cellular and Molecular Medicine
3 Blackfan circle, Room 3099
Boston MA 02115
Email:
hao.wu@childrens.harvard.edu
Dates at MBL:
6/16/2014 - 8/1/2014
Research Title:
Research
Description:
HCIA/HHMI Summer Institute
Room Assignment: R-319, 320, 321
Meg Younger
MBL Association:
PI-Grass Fellow
Institution Contact Information:
UCSF
Biochemistry and Biophysics, Davis Lab
GDBS, 1550 4th St
Rm 445, Box 2822
San Francisco CA 94158
Email:
meg.younger@gmail.com
Dates at MBL:
5/26/2014 - 8/30/2014
Research Title:
Research
Description:
Processing of human odorants in the mosquito antennal lobe.
Room Assignment: R-201,223
Female mosquitoes show intense human host-seeking behavior. Attraction to components of human odor such as lactic acid is
enhanced by carbon dioxide (CO2). This sensitization to otherwise unattractive human cues is poorly understood. The aim of my
project as a Grass Fellow is to understand how human odor processing occurs downstream of Olfactory Sensory Neurons in the
brain of the Dengue fever mosquito, Aedes aegypti.
43
Joshua Zimmerberg
MBL Association:
Principal Investigator
Institution Contact Information:
NIH
NICHD
10 /10d14
10 CENTER DRIVE MSC 1855
BETHESDA MD 20892-1855
Email:
zimmerbj@mail.nih.gov
Dates at MBL:
6/15/2014 - 9/7/2014
Research Title:
Research
Description:
Membrane biophysics and lipidomics of the synaptic vesicles of the Woods Hole squid
Room Assignment: R-309
The goal of this project is to continue to understand the role that lipid composition plays in determining the physical parameters of
the biological membrane, particularly in the contect of the nervous system. Although many theories predict a central role for lipid
rafts in the organization of the synaptic membrane, we have discovered that the synaptic vesicle membrane of the squid optic lobe
has neither the right lipids to form traditional rafts nor the degree of lipid order needed to form liquid ordered domains. We
hypothesize a novel domain, for which last summer (2011) we got preliminary data. In summer 2012 we continued this project,
mapping the phase diagram of the synaptosome, which had the same lipid distribution as the synaptic vesicle. We are ready for
physiological and behavioral experiments on squid and squid axons as a function of temperature.
Steven Zottoli
MBL Association:
Principal Investigator
Institution Contact Information:
Williams College
Biology
59 Lab Campus Drive
Dept. of Biology
Williamstown MA 01267
Email:
stephen.j.zottoli@williams.edu
Dates at MBL:
5/1/2014 - 8/31/2014
Research Title:
Research
Description:
Morphological and physiological studies of dorsal cell/supramedullary neurons in teleost fishes
Room Assignment: R-213
Even though the presence of dorsal cell/supramedullary neurons have been known for over 100 years, their function remains a
mystery We continue to study the physiological and morphological properties of these large and accessihle neurons to better
understand the neuronal basis of behavior.
44
LastName
Alford
Armstrong
Augustine
Baker
FirstName
Simon
Peter
George
Robert
AcademicRank
Professor
Professor
Professor
Professor
Institution
University of Illinois at Chicago
University of California
Duke University Medical Center
New York University Langone Medical Center
Balmer
Bennett
Berger
Bezanilla
Bloom
Tim
Michael
Christopher
Magdalena
Ona
Graduate Student
Professor
Associate Professor
Associate Professor
Assistant Professor
Bodznick
Bohorquez
Brady
Brangwynne
Burgess
Chang
Chen
Cohen
David
Diego
Scott
Clifford
David
Fred
Christopher
Lawrence
Professor
Postdoc
Professor
Assistant Professor
Professor
Professor
Graduate Student
Professor
Colin
Colon-Ramos
Costello
Dzakpasu
Echeverry
Sean
Daniel
John
Rhonda
Fabio
Associate Professor
Associate Professor
Professor
Assistant Professor
Graduate Student
Field
Fritzenwanker
Christine
Jens
-Postdoc
Furlow
Gadsby
Garcia-Arraras
Gatlin
Gillis
Gladfelter
Goldman
Gonczy
John
David
Jose
Jesse
James
Amy
Robert
Pierre
Professor
Professor
Professor
Assistant Professor
Postdoc
Associate Professor
Professor
Professor
Goshima
Grant
Gray
Green
Groh
Gross
Hammarlund
Hershko
Gohta
Philip
Jessica
William
Alexander
Jeffrey
Marc
Avram
Professor
Professor
Postdoc
Professor
Postdoc
Associate Professor
Assistant Professor
Professor
Huh
Yeowool
Graduate Student
Georgia State University
Albert Einstein College of Medicine
University of Vermont
University of Massachusetts Amherst
Feinstein Institute for Medical Research;
Hofstra North Shore
Wesleyan University
Duke University Medical Center
University of Illinois at Chicago
Princeton University
Boston College
Columbia University
Albert Einstein College of Medicine
Korea Institute of Science and Technology/
Yale
Roger Williams University
Yale University
Providence College
Georgetown University
Center of Advanced European Studies and
Research
Harvard Medical School
Hopkins Marine Station of Stanford
University
University of California, Davis
Rockefeller University
University of Puerto Rico
University of Wyoming
Dalhousie University
Dartmouth College
Northwestern Univ. Medical School
Swiss Federal Institute of Technology,
Lausanne (EPFL)
Nagoya University
National Institutes of Health
Harvard Medical School
University of Chicago
Technical University of Munich
University of Texas at Austin
Yale University
Technion- Israel Institute of Technology, Fac.
of Medicine
Korea Institution of Science and Technology
Jonas
Jorgensen
Kaczmarek
Karlstrom
Kaupp
Kingston
Kittelberger
Kovar
Lee
Elizabeth
Erik
Leonard
Rolf
U. Benjamin
Alexandra
James
David
Wei-Lih
Associate Professor
Professor
Professor
Professor
Professor
Graduate Student
Associate Professor
Associate Professor
Associate Professor
Yale University
University of Utah/HHMI
Yale University
University of Massachusetts
Research Center caesar
UMBC
Gettysburg College
The University of Chicago
UMass Amherst
Llinas
Llobet
Rodolfo
Artur
Professor
--
Lowe
Christopher
Assistant Professor
Maienschein
Malchow
Martinez-De Luna
Mease
Mensinger
Miller
Mitchison
Morfini
Nakamura
Oakey
Pant
Parker
Pereda
Rome
Rosa-Molinar
Rose
Rosen
Rosenthal
Jane
Robert
Reyna
Rebecca
Allen
Andrew
Timothy
Gerardo
Tetsuya
John
Harish C
Roy
Alberto
Lawrence
Eduardo
Robert
Michael
Joshua
-Associate Professor
Postdoc
Postdoc
Professor
Professor
Professor
Assistant Professor
Postdoc
Assistant Professor
Professor
Professor
Professor
Professor
Associate Professor
Associate Professor
Professor
Assistant Professor
Rost
Benjamin
Postdoc
Selvin
Sloboda
Sluder
Song
Springer
Paul
Roger
Greenfield
Yuyu
Timothy
Professor
Professor
Professor
Postdoc
Professor
Tamm
Terasaki
Treistman
Vale
Watanabe
Waterman
Wilhelm
Wu
Sidney
Mark
Steven
Ron
Shigeki
Clare
Jim
Hao
Professor
Associate Professor
Professor
Professor
Postdoc
-Associate Professor
Professor
Younger
Zimmerberg
Zottoli
Meg
Joshua
Steven
Postdoc
-Professor
New York University School of Medicine
Bellvitge Biomedical Research Institute /
Univ. Barcelona
Hopkins Marine Station of Stanford
University
Center for Biology and Society
University of Illinois at Chicago
SUNY Upstate Medical University
Technical University of Munich
University of Minnesotat Duluth
HKUST
Harvard Medical School
University of Illinois
The University of Chicago
University of Wyoming
NINDS/ NIH
Univ of Colorado
Albert Einstein College of Medicine
University of Pennsylvania
University of Puerto Rico-Rio Piedras
North Carolina State University
UT Southwestern Medical Center
Universidad de Puerto Rico/Recinto de
Ciencias Medicas
German Center for Neurodegenerative
Diseases (DZNE)
University of Illinois Urbana Champaign
Dartmouth College
UMass Medical School
HHMI/Yale Medical School
Childrens Hospital/ Harvard Medical School
Marine Biological Laboratory
University of Connecticut Health Center
Univ. Puerto Rico Medical Sciences
UCSF
University of Utah
National Institutes of Health
UC San Diego
Boston Children's Hospital and Harvard
Medical School
UCSF
NIH
Williams College