ORDER OF DRAW TUBE GUIDE
Central Laboratory: 41 Victoria Parade, Fitzroy (03) 9231 2888
NOTE: Tubes are listed in recommended order of draw - Blood cultures drawn first.
Closure &
Tube Content
Determinations
General Biochemistry Tests (LFT, U&E’s, CRP etc), Lipid Studies (Chol, Trigs,
HDL), Cardiac Enzymes, Troponin, TFTs, PTH, Cortisol, Most Therapeutic
Drugs, Antibiotic Assays, Serum Electrophoresis, Vitamin B12, Folate, Viral &
Microbial Serology (HIV, Hepatitis, EBV, CMV, Syphilis, Rubella,
Toxoplasmosis, Varicella Zoster, HSV etc), Autoimmune Serology, Anti - platelet
abs, Anti - neutrophil abs, Anti - leucocyte abs, HITS screen
Serum Separator
(Gel)
8ml
Mix by gentle, figure of eight inversion.
Troponin – NB: Lithium Heparin tube required for Troponin on inpatients
and patients receiving IV heparin.
INR (Prothrombin), APTT, Protein S, Protein C, Lupus Anticoagulant (x2 tubes),
Antithrombin III, Activated Protein C resistance, Coagulation Studies, D-Dimer,
Disseminated Intravascular coagulation
Sodium Citrate
2.7ml
Instructions
Refrigerate and Transport at 4°C within 2-3 hours. DO NOT FREEZE.
Allow tube to fill to completion as
the ratio of Blood to Anticoagulant is
critical.
Mix by gentle, figure of eight inversion.
C-Peptide, Insulin – Refrigerate and Transport at 4°C. DO NOT FREEZE.
Lithium Heparin
(Gel)
8ml
St Vincent’s Hospital ED, ICU and CCU Patients AND Patients Receiving IV
Heparin: General Biochemistry Tests (i.e. LFT, U&E’s, CRP, Troponin etc).
Mix by gentle, figure of eight inversion.
Karyotyping
Allow tube to fill to completion.
Aluminium
K2 EDTA
6ml
EDTA
4ml
FBE (Hb, WCC, Diff, Platelets), Blood film, Reticulocytes, ESR, Direct Coombs
Test, HLA-B27, Cell Markers (Lymphocyte Markers), T-Cell Subsets (CD4
Count), Red Cell Folate, Platelet Associated Immunoglobulin (x2 tubes),
Cyclosporin, Tacrolimus, Sirolimus, Flecainide, Amiodarone, Perhexiline,
HbA1c, Hb Electrophoresis
Mix by gentle, figure of eight inversion
Mix by gentle, figure of eight inversion.
Hemochromatosis, Factor V Leiden, Prothrombin and JAK-2 Gene analyses,
Thalassaemia gene analysis.
Cross Match, Blood Group & Antibody Screen
Hand written labelling is required on this
tube. Blood Bank Department will not
accept printed labels.
EDTA
6ml
Allow tube to fill to completion.
Glucose, Glucose Tolerance Test, Glucose Challenge Test, Alcohol, Lactate.
Mix by gentle, figure of eight inversion.
Fluoride Oxalate
2ml
HLA Typing, HIV Viral Load, Hep C PCR, Hep C Viral Load,
Hep B Viral Load.
Mix by gentle, figure of eight inversion.
ACD
10ml
HAZARD WARNING: (Never inject blood into the tube from needle and syringe.)
ALWAYS LABEL TUBES LEGIBLY - SURNAME, GIVEN NAME, DATE AND TIME OF COLLECTION, DATE OF BIRTH, COLLECTOR’S INITIALS.
For other tests please consult the online Pathology Test Manual at http://path.svhm.org.au/
or contact St Vincent’s Pathology call centre on 03 9231 2888
General Feedback: pathologyfeedback@svha.org.au
PA-COL-F-61 v11 19/3/2015