Figure 7 b
The blank fields on the inventory sheet must be completed on the inventory sheet as soon as the shipment has arrived in the laboratory.
Decay Table for P-32
Isotope P-32
alpha dCTP
Activity Ordered 0.250000 mCi
Permit Holder
Dr. N. Person
Permit No:
2001-01
Date Ordered:
2012.05.30
Date received: _2012.06.06_
Before
Days
After
10
20
30
40
50
60
70
Reference date: ____June 9, 2012_______
Activity on Ref. Date: ____9.25____MBq
Volume Rec’d: ___0.025_______ ml
Lot No: ___XT 123__________
Concentration: _____370_____MBq/ml
1.000
0
1.000
0.616
0.379
0.233
0.144
0.088
0.054
0.033
1.049
1
0.953
0.587
0.361
0.222
0.137
0.085
0.052
0.031
1.101
2
0.906
0.559
0.344
0.212
0130
0.080
0.049
0.029
1.156
3
0.865
0.532
0.328
0.202
0.124
0.077
0.047
0.027
1.214
4
0.824
0.507
0.312
0.192
0.118
0.073
0.045
0.025
1.274
5
0.785
0.483
0.297
0.183
0.113
0.069
0.043
0.023
1.337
6
0.748
0.460
0.283
0.174
0.107
0.066
0.041
0.021
1.404
7
0.712
0.436
0.270
0.165
0.102
0.063
0.039
0.019
1.474
8
0.678
0.418
0.257
0.158
0.096
0.060
0.037
0.017
1.547
9
0.646
0.396
0.245
0.151
0.093
0.057
0.035
0.015
Carcass
swipe
checks
Upon Arrival in the lab each vial in this shipment must be swipe tested for contamination AS SOON AS POSSIBLE.
Vial 1 _XX__ NET CPM Vial 2 _____ NET CPM Vials 3 _____ NET CPM Vial 4 _____NET CPM
Storage location of stock vial (building & room) _____SA 013______ Fridge or Freezer # ___number 1_
Stock vial
Product
Room where
material is
used
Procedure
User
Name
Date
μl used
μl remain
μl stored
13
13
17
13
Procedure 1(end labelling)
Procedure 2 (hybridization)
Procedure 3 (library)
Procedure 1 (waste disposal)
Able
Bill
Bill
Able
06/ 06
06/ 13
06/ 18
06/ 18
4
3
4
-
21
18
14
-
0.1
0
0
13
Stock Vial Disposal
BJ
07/ 26
Record dilution factor under procedure
14
Waste Disposal
Solid
Liquid
μl
can #
μl
can #
3.6
2
2
0.1
104
202
202
202
0. 3
1
2
RA 103
RA 001
RA 303
Activity in Stock Vial Disposed
LS vials
√
√
√
0.528 MBq
ACTIVITY REMAINING IN THE LABORATORY
Liquid Waste Pick-up Information
Solid Waste Pick-up Information
0
Pick-up Date
Can #
Activity in Solid (MBq)
Pick-up Date
Can #
Activity in Liquid ( MBq)
June 14 , 2012
July 5 , 2012
104
202
1.045
0.429
June 14 , 2012
June 21 , 2012
July 5 , 2012
RA 103
RA 001
RA303
0.087
0.207
0.209
Waste Pick-up : Activity = total microliters (μl) disposed in each container X 0.001 X original concentration of stock solution X decay factor (number of days between ref. date and pick-up date).
Send inventory sheet to Safety Services once all waste has been removed from the laboratory. Keep a photocopy for your records.
Each time radioactive materials are aliquoted, used, stored, or disposed; information must be recorded on the Radioisotope Inventory Sheet. Indicate the Room
where the materials will be used, the name of the Procedure that will be performed (end labelling, sequencing, western blot, PCR, hybridization, etc.), the name of
the individual (User Name) performing the procedure, and the Date the procedure was done. When materials are removed from the stock vial the volume used
and the volume remaining in the stock vial is to be recorded. The amount of waste in each container needs to known and recorded. At the end of the procedure
the individual MUST complete a contamination survey, record on the Radioisotope Inventory Sheet that swipe checks were completed, and place the results in
the Contamination Logbook.
Figure 7 b
For Procedure 1
4 μl were removed from the stock vial therefore: 25 μl – 4 μl = 21 μl remain in the stock vial
the 4 μl removed from the stock vial were used in an experiment and the materials were distributed in the waste stream as
= 0.1 μl as stored material in a gel (that will be disposed of later) + 3.6 μl was placed in the solid waste container 104
+ 0.3 μl was placed in the liquid waste RA 103
For Procedure 2
3 μl were removed from the stock vial therefore: 21 μl – 3 μl = 18 μl remain in the stock vial
the 3 μl removed from the stock vial were used in an experiment and the materials were distributed in the waste stream as
= 2.0 μl was placed in the solid waste container 202 + 1.0 μl was placed in the liquid waste RA 001
For Procedure 3
4 μl were removed from the stock vial therefore: 18 μl – 4 μl = 14 μl remain in the stock vial
the 4 μl removed from the stock vial were used in an experiment and the materials were distributed in the waste stream as
= 2.0 μl was placed in the solid waste container 202 + 2.0 μl was placed in the liquid waste RA 303
Activity calculations for the waste disposal:
Activity = total microliters (μl) disposed in each container X 0.001 X original concentration of stock solution X decay factor (number of days between ref. date
and pick-up date).
Can # 104
Activity = 3.6 μl X 0.001ml/μl X 370 MBq/ml X 0.785 is the decay factor for 5 days from the decay table.
= 1.045 MBq
Can #202
Activity = (2+2+0.1) 4.1 μl X 0.001ml/μl X 370 MBq/ml X 0.283 is the decay factor for 26 days from the decay table.
= 0.429 MBq
Can # RA 103
Activity = 0.3 μl X 0.001ml/μl X 370 MBq/ml X 0.785 is the decay factor for 5 days from the decay table.
= 0.087 MBq
Can # RA 001
Activity = 1.0 μl X 0.001ml/μl X 370 MBq/ml X 0.559 is the decay factor for 12 days from the decay table.
= 0.207 MBq
Can # RA 303
Activity = 2.0 μl X 0.001ml/μl X 370 MBq/ml X 0.283 is the decay factor for 26 days from the decay table.
= 0.209 MBq
Stock Vial disposal
Activity = 14.0 μl X 0.001ml/μl X 370 MBq/ml X 0.102 is the decay factor for 47 days from the decay table.
= 0.528 MBq