Biology 321 QUIZ#1 Spring 2011 25 pts. NAME______________________________ 1. (3 pts.) The hormone gibberellin is a major determinant of plant height and dwarfism can be caused by a failure to produce or to respond properly to this compound. Many different gene products are required for the biosynthesis of gibberellin and for the proper cellular response to its presence. Given this information do you think that dwarfism in plants can be inherited as a single gene trait? Circle: YES Then support your answer with a 1-2 sentence explanation. No credit if no explanation. Student Answers: If a mutation in one gene inhibits the entire pathway needed for the synthesis of gibberellin, then it can be a single-gene trait. (One mutation causes a phenotypic change). If one gene product in the sequence is disrupted it could cause dwarfism. For example, the biosynthesis of gibberellin may function properly, but the cellular response may be incorrect. Therefore that single gene for cellular response could cause dwarfism. 2. (5 pts.) American elms (Ulmus americana) lined many city streets before Dutch elm disease killed most of them in the mid 20th century. It has been accepted for many years that American elms are tetraploids (4n=56), but there have been persistent rumors of elm trees that were triploids or diploids. In the 1990s it was shown that a resistant strain of elms found in Washington DC was 3n = 42. More recently diploid elm trees (2n=28) have been found in the wild growing alongside the tetraploids. Circle True or False for each statement. Answer false any part of the statement is false. If there are two statements, the first statement is true and you are to decide if the second statement is T or F. F The information given above implies that the elm strains differ with respect to the number of different chromosomes (double-stranded DNA molecules) per genome copy. F Each product of meiosis I in the tetraploid strain would have 14 chromosomes and 28 chromatids T Each product of meiosis II in the diploid strain would have 14 chromosomes and 14 double-stranded DNA molecules. T A commercially valuable fungal-resistant strain called Jefferson has been derived from the 3n strain. Asexual propagation of this strain via mitosis does not present a problem even though the uneven number of homologs means that some chromosomes will not have a pairing partner. T Although there is no direct evidence, a wild diploid strain may have may have been the original source of the disease resistance seen in the triploid stock. This speculation makes some sense since an accidental cross of a 4n elm with a 2n elm would produce a triploid offspring. 1 3. (9 pts) You are exploring the inheritance of two traits in summer squash. Different parental plants (all of unknown ancestry) were used in each cross. Trait fruit color fruit morphology (shape) Alternative phenotypes green or yellow disk or sphere Number of progeny in each class Parental phenotypes 1 yellow disk y y d c c m m X green sphere green disk 0 0 d yellow sphere yellow disk 500 500 y green sphere w c c ms md cy cw md md 300 100 cw cw m s m d 2 yellow sphere X y w s c c mm d green sphere 300 w 100 w w c c ms m- w c c md md y w c c ms m- cy cw md md cw cw m s m d a. Which color form is dominant? __yellow_____ For part b, fill in the blank from the choices below. No explanation needed. b. Dominance for this trait could be determined from cross 1 only cross 2 only either cross c. Which fruit shape is dominant ? ____sphere__________ d. Dominance for this trait could be determined from cross 2 only cross 1 only cross 2 only either cross d. For each cross indicate the genotypes of the parents and progeny in the space underneath the phenotype. Use these allele designations OR define your own alleles symbols below. cy= yellow cw = green md = disk ms = sphere OPTIONAL: Use your own alleles symbols. Define them here: 2 4. (4 pts.) Examine the diagrams shown below which were taken from one your assigned problems in Chapter 3. Each line represents a single molecule of double-stranded DNA, which will segregate as indicated. A. 3 pts: +1pt for each correct answer; -1 pt for each incorrect answer Diagram #1 (below). Choose all correct interpretations. a) This drawing shows a 1n=2 cell undergoing mitosis b) This drawing shows a 2n=2 cell undergoing mitosis c) In meiosis II in an organism of genotype AABB, all MII divisions will look like this d) In meiosis II in an organism of genotype AaBB, ½ of MII divisions will look like this e) In meiosis II in an organism of genotype AaBb, ½ of MII divisions will look like this Ó Ô B. 1 pt Examine Diagram #2 (below). This drawing is consistent with which of the scenarios listed below? Choose all correct interpretations. a) Mitosis in a 2n=4 cell of genotype AaBb b) Mitosis in a 4n=8 organism of genotype AaBb c) Meiosis I in a 2n=4 organism of genotype AaBb d) Meiosis I in a 4n=8 organism of genotype AaBb e) None of these interpretations are correct Ó Ô 5. (4 pts.) a. A friend of yours needs to determine the genotype (AA or Aa) of a plant that shows the dominant phenotype for a single-gene trait. He allows the plant to self and looks at 10 offspring. If the plant is heterozygous (Aa), what is the probability that all 10 progeny show the dominant phenotype? Show your work and circle your answer. Just set the answer up. Aa X Aa à ¾ chance dominant phenotype vs ¼ chance recessive Probability that all 10 offspring show the dominant phenotype is [3/4]10 b. You argue that a test cross would be better at revealing the presence of a recessive allele among a small number of progeny. Briefly but explicitly explain your argument. Be sure to state what a test cross is. Test cross = Aa X aa (tester) This cross is more likely to reveal the presence of a recessive allele in any given offspring since the probability of seeing a recessive offspring is ½ versus the ¼ probability of seeing a recessive offspring in the self progeny of an Aa plant. The probability that an Aa X aa cross will reveal only dominant phenotypes among 10 offspring is [½]10 -- much lower than [3/4]10 NOTE: Many of you indicated that the probability of seeing a recessive offspring in an Aa X aa cross was ½, but didn’t state that this probability is higher that what 3 you would expect from a self-cross. On future quizzes and exams, but sure to make these connections explicitly. 4 Page (pts) 1 (8 ) Score 2 (9) 3 (8 ) Total (25) 5