Protein Standards
•
'D
Invitrogen"
life technologies
Featuring the Gold Standards
in Band Identification
With Invitrogen's protein
standards you can expect:
• Clear band resolution
• Accurate, easy-to-read
results
• A time-saving, load-and­
go format
•
t:'
Invitrogen·
life technologies
Setting the Standard
nvitrogen's complete line of protein markers sets the standard in electrophoresis
band identification . Eight different protein standards are available, each with unique
advantages, all offering maximum convenience. With every standard, you'll get:
• Unambiguous identification-proteins resolve into clear, sharp bands for precise results
• Wide size range of protein markers- enables you to identify diverse protein molecular
weights easily
• Load-and-go format- standards are supplied ready to use; with no need to mi x,
redu ce, or heat before using
• Consistent high quality-standards are strictly quality controlled to ensure consistent
band intensity
Table of contents
Description
Applicat ion Overvi ew . Pre-Sta ined Molecular Weight Sta ndards . Page
..3
. .. .. . . .. 4-7 Mult iMar k· Mulli-Co lo red Sta nd il l·d . . 4
See ll lue 1>lu s2 Pre-Stain ed Sta nd clrd . .5 Scc Blu e Pre-S tai ned Sta nd ard Bench Ma rk Pre-Stain ed Prot ein Ladder .. Unstained Mol ec ular We ight Standards .. ... 6 . 7
. . .. 8-9 Mar kl r Unstain ed Stil nd a rd .8
BenchMd rk··· Prot ein Ladder . .9 Ma g icMark " Wes te rn Protein Sta ndard .. SERVA JEF 3-10 Marker .. Ordering In formati on . 2 I I 800 955 6288
10 . . ... 11 . . 12
Application Overview
A standard for every application
A broad selection of protein standards is available
accurate size estimation than pre-stained standards.
to mee t your electrophoresis needs. Whether you're
Pre-stained standards, however, are well suited for
J pproximating molecular weight, verifying transfer
confirming electrophoresis runs and estimating
eifi ciency, or determining protein isoelectric point,
transfer efficiency. They also offer good molecular
yo u 're sure to find a standard that meets your appli­
weight approximation if they are calibrated accord­
( <lt ion need ' (,rable 1). Each proteill standard is sup­
ing to the gel/buffer system in llse.
plicll in a ready-to-use format, eliminating tile need
to dilute, mix, or heat before loading.
Marker for westem blot
Molecular weight tandards for 5DS-PAGE
easy and convenient means to accurately estimate
MagicMark' Western Protein Standard provides an
Protein molecular weight standards provide the
protein molecular weight directly on western blots.
meJ ns to estimate moleculal' weight as well as to
You can visualize MagicMark' standard bands simul­
confi lm electrophoresis and transfer runs in SDS­
taneollsly with your target protein using the same
PAGe (sodium dodecyl sulfate polyacrylamide gel
antibody conjugate and protocol.
ele ctrophoresis ). l3y constructing a standard curve
with a series of standards, you call estimate tile
IEF marker tor Isoelectric focusing gel
ap pare nt molecular weig ht of a sample protein
The SERVA Liquid Mix IEF Marker enables you to
based
determine the isolectric point (pJ) of unkl10wn pro­
0 11
its relative mobility. Unstained protein
molecll iar weig ht standards (i.E'. , Mar-kI2' Unstained
tein samples on horizontal and vertical isolectric
Sl,l ndard, BenchMark" Protein Ladder) provide more
focusing (rEF) gels.
Table
~
1 -
Advantages of Invitrogen's protein standards
'.
,
• • • • l1li­
Multi",lJI k Mutti- Colored SIJndJrd
4 -250 kD a t
Multi-colO'e d
SeeBl ue r lus 2 Pre :i tai ned Stan d.) rci
4-250 kDa 1
Sl1,l[ply resolved ban ds , including two colored bands
for easv ar1,1lysis
SeeBlue t' re-Sta ined S!.lncLud
1-250 kDa t
Sh,lrpest, most co rl sistent pre-stairleli bands
10-190 kOa t
SI1arp blue bands with one pirlk band fm e.lsy
Bl'llchlYLl rk I'n,,-Stc1irled Protein Ladder
N
ba nds for ahl g],lnce id entific.lti on
band identification
i\1,u k t2 Unstai ned Sta nci .l rd
2.5-200 kDa
i\ccmate estimation of lllolecular weight with the
broadest molecular weigbt range
HCllcilM.ll k Pro lL'in Lldder
10-220 kDa
i\ccurate estimation of molecular weight includ ing two
triple-inte ns ity bands for easy referen ce
Magicivt.rrk Western Prol ein St,lndard
20-120 kDa
i\ccur,lte molecular weight estimation directly un
western blots
SF RVA li q uid Mix IU' Marker
pi 3.5-10.7
Accurate determination of protein isoelectric points
www.invitrogen.com I3
•
•:. Invitrogen
life technologies
For the easiest molecular weight band identification
The MlIlli\l,lIJ.. 'vll1l li Co\nrl'U
colorful alterncllive
lO
SI,md,mJ
gives you a
Because eac h band is a differellt co lor, you'll be abl e to
eas il y and immediatel y idenlif), the Ill oiecu l" r weight of
blu e, pre-sla ined mol ec ular weight
markers. It conSlSIS of 9 mulli-colored bands (Figure ll.
each pro tein.
Figure 1 - Apparent molec ular weights' of the MultiMark " Multi-Colored Standard
5DS PAGE System
... Protein
Tris-Glycine
Tricine
NuPAGE"
Bis-Tri s (MES)
NuPAGE"
Bi s-Tris (MOPS)
NuPAGE '
Tris Acetate
:Vl yos ill
250
208
185
IRS
20 ')
Pho"pho rvIJ'" B
148
105
98
97
III
Clut,llilic Debytiwgc ll cl St'
60
5"
52
S2
52
Carbon ic Anhydr,l se
42
34
31
31
.,'1
Myogluhin -'1 II"
Iii
• 1
,.
19'
21'
11 / .1 M yoglobill - R"d
22
I~
1"
I 'J'
11 / ~l
Lys07.y n1c
17
13
II
12
n/ Ll
I\protillin
6
7
6
11 / ,1
11 / .1
Insulin, B Cil .-li ll
4
4
3
11 / ,1
n/ .1
Approximale Mull'c ulclr \'\'cigllls (k[) a)
"ll I'M ,L 4 I': " BI~ · Trj,
CI.'I \\' i MFS S[1S Bu fit ' l
\k4 \, ' 'Jll t:' :'- ! lIgr.llhm 1I,liltllll" l,' ,\l\vg!nhJl I R'-'d JJl(i
l lidu'" Sy.qH.'m". Ull1~
.lH'
rjolo\'('r~r rllu
the NIIP/\GI:. lh5--Tlb l\trS .lUli ."10P5 UUIH.'rs ci"nn r.'Htld 10
IIJ~
In.. . t;lvrtllf' .llld , Il.\ i.1J.1 uiotl P~lth'ln ~ ill '('\'(1 " , 1 hu lkl <: 1 ..,1,'111:-. 1H~ :;-.hCl\\ II ~)l'~I1lJ"1" ~)IClI(.'1t1 b.HlH'" Wi ll h.l\'f' dllll'r~1I I11nlnlltl(>... 111 l.hlr...'H.'JH SDS P/\~[ h~rth'1" :-:"Sh'I1l:>. 111 1" IIlllff'
illldtJlMriUl1 011 Ihi:, I' hC'IWl\lt'lInn , r o nl.)1 I . ' I ~ , hr1li:,1 . $0\ lel' f\l'IUII'!~f' I1t "'li \l ~ .:11 H.O() '1 ~S
(,."!H6. ('XI 2 \,r rt'vi t"''''
lit JT'h·ll'L'IJIM wl'igJ1t M,lU( 1 .Hl1~ (or ~hlh H'l lI 1111 1ff'r " \"Wtu.... nil our wl'tl ~ill' ~I! w w will v i!n,)og€'11 ClllH
4
I
I 800 955 6288
l)ll'
,ech rllul !:OIL l'nhllcd '"/\ccnr.Htl
Crlllh',1 11 1.11 '
Pre-stained ,Molecular Weight Standards
j'
Sharp bands and easy analysis Th e 'lt,t't:llIl' rllb2 I'rl' Sl.li l1 t'tI SIJlld.HcI co nsist s
(F igure 2) . The two co l.ored band s make il easy to
of 10 pre-s ta ined pro lein markers -8 blu e and
immediat ely ide nlify th e pro tein mark ers.
2 co iored - Illat re so lve into sl1arp dis tin ct bands
Figure 2 - Apparent molecular weights' of the SeeBlue " Plus2 Pre-Stained Standard
5DS-PAGE
Sy ~ tell\
Protein
Tris-Glycine
Tricine
NuPAGE'
Bis-Tris (MES)
NuPAGE '
Bis-Tris (MOPS)
NuPAGE '
Tris-Acetate
M yo,;i ll
2:;0
210
I RS
191
2lO
135,\
98
78
62
64
71
CIUIJlIlic DehyfirngE'IlJ sc
64
5)
49
51
55
/\ Ico hul n cil ytirogel1Jse
50
45
38
39
41
C.lrbo llic J\lIilyclr,lsl'
36
34
28
28
n/ a
jv!vuglub ill -Rt'r1
22
I'
17
I 'l
I1/ J
[.ysozy m e
16
16
14
14
II/a
r\ pro l i II i 11
6
7
6
Il/ a
II / a
IIISl1l ill , 13 eiJ ,l in
4
4
3
11 /.1
11/,1
I'lw,;phul-yl,ISl' B
...
Approx im ale Mo lecular
Wc i ~ hl s
(kD,l)
~'lIit"I!?111
SuS- rACe
'"PAG I~ 4- 12 t;~, \3i ~- TI is
l;.'1 \\ ' \ ,11:::; SDS Rul IL'1
• \l ll~IAtI!Jl l IMlh' n" 111 <.I'velll
i llh' t1~I.t1Hll' 1) 1\ 111)"
h!:frt'r "; \",11'111'1 HI.: ~hoW III'\"r,1II~t pnrtl'illl'MHI, wlll h.J" ~ Jlll('(C'11 1 rn( tttiJ i llt~ III
ph,·'U,l1111'nvtl. "(':111 1.1(1 .1 h'. tnll~JI ~ i ' I \'lce 1-: t:'llrl.'''4,,'r11,Hlvl.. .11 suo I);~ (')'lI'1 , ~,~ 1 ,1 UJ l~v l\.'w
the fl"chM!(.! 1 UuW
bu fh.~f ~)~lt'IW~ . ru /
"HIl t'
MUIIIl'Ll "J\t'LW.1I.~ LJllln,11ioC'lll
·)1 llndt.'c'lJku \W l ~h l ~ t .lOrl.lrrl" tnr (hili '~'111 hl1 l1,. >''i.. I~ m ::- '' VII nlll \\'t.'/I .;.llI' U \\,Ww .lll\ Irluge-II r ulU ,
www.invitrogen.cOlll
I
5
•
.D
Invitrogen
life tec;hnologies
Sharpest band resolution
For silctrp , consiS lent pre-s lained bands, th e ')lct' Blut'
blue-Ihal provide Illgil reso lut ion in any SDS-PACE sys tem
I'll' Slailll'd
(Figure 3) . Since SeeB lu e is suppli ed pre-stained, th ere i:;
t! is th e molecu lar weight standard
S[,mU dl
of choice. It consists of 9 indi vidual protein band s-all
no need to s tain th e gel in order to visualize Ih e stJndard .
Figure 3 - Apparent molecular weights* of the SeeBlue ' Pre-Stained Standard
SOS PAGE System
Protein
Tris-Glycine
Tricine
NuPAGE '
Bis-Tris (MES)
NuPAGE < Bis-Tris (MOPS) NuPAGE Tris Acetate M yo;;in
250
210
188
191
210
8 51\
98
78
62
64
71
CI UIJmi c D l'h ydrogen,l se
64
SS
49
51
5S
Alcohol Oehyd rot;E%lSe
50
45
38
39
41
Carbonic !\ ohyd rd ,;e
36
34
28
28
11 / ,1
Myoglobin
30
23
18
19
11 / ,1
I .y,;u zy me
16
16
14
14
11 /.1
Aprol inin
6
7
6
n/ .1
o/ a
Insulin , 13 chain
4
4
3
n/ ,\
n/J
A
Allprox ilnJIC MoleculJr Welgh ls IkO.ll
t>.' uPAC C I
L~
;, 13i ~- fIlS
Cel WI ),lI'S SOS Buffer
\ 11!:!1.11111 p..t l h'W", III
l'~ l! h, I I'u ffl'l :-o.\·~ 1 t' 1I1 !-.lrr :- ho wil on:,Hl~; l' jl ltlll' l lI h, III J ~ ,.\ d1 11.1\'(" d iHC' IC'l lllIlnhl lllk" il1l liitC'I't '11 1 ~f lS l'r\l; 1
ml"o r. 11.1,I\I') Ill ) Ihh I I !U,1Il(lIlII!!1lJll, t:Oll l.I{"1 I ' 1{t("h IlIL·a J Sl'f\'!(:t: H'1Ift'c;\'I' t.1I Ivt ,It A.ll0
'1
6
mrdC'1
111.11
'.\ ,:Lglll
~ 1. ' llI !,t rd'l; fi ll lhtll'I t.' !1t I'Wlttl ~Y ""::'I1l'i "
I I 800 955 6288
on
'Jr;,
6~~H\, l,';\.!
111111" 1 !-\'~I t:m~. r ut trt l ll13
.l III I l~\' il~\\' the ft'd tn h',tl Imlt' 1'" 1I 1111t'l l ··,\\..'(tlr,11-.' cl llbrJlIllI'
P1I r Well <:'1 1. ' ,11 w ww Il\ l! ro~C'n .n1l11
Pre-stained Molecular Weight Standards
Monitor electrophoretic separation in real time T he Ikllcll~tJrl<
['rlL
S1.1illL'd PW It!in L.1ddC'r al lo ws
T he standa rd prot eins are affinity-purified ilnd
you to monitor the progress and qualit y of an
cov,llently coupl ed w ith dye. You'l l see superb band
electrophoretic sepa ration. Like other pre-stained
sharpn ess (figure 4), ge t easy orient atIO n w ith a
pro tein sta ndards, you can also llse the BenchMark"
pink reference band, and approximate molecular
l're- Sta ined Ladd er to estimat e the efficien cy o f
weight wit hout diffi culty
proll'in trJl1sfer w hen perform l\1g western blotting.
Figure 4 -
BenchMark ~
kfl,
~
190
~
12U
Pre-Stained Protein Ladder
k 1,1
." I tl(l
o
1211
~
HS
~(JO
~
SO
.-, \0
. 4()
" 15
.... 10
. \l1'fllI>\ 11 11111 ' dllntHOplh)h:" 10 Ih ~ prntl'lll:-. .lll('('h ·ht'lr'llp.\l Ci:1 llinit'CLI!.lr
wdght ill ~p:-; ]!A( ~F H'It1 l h '(' i n 11Il~1 "IIll'd S\,lUlLml:-. . •.•l lll h,lIld in IhC' (Uf' :; IAilh;.lJ
.uMrr j... ('J.hbr..1 'I:d 11~j.l i BS I 111I~I.l;rll'll BI'nrll;-..t.lI
fi 'QIC'iI1 Lllhlt.'r Illl 1 l.:'II ':,
R("j .1fld rill' '~!'ll.,lrt!l)r m{) l ~fll l.l r \\'('i~ ! H b rrpcu l ~1 on Il\t· plOdUfI
lri~ ' <; ;\Till(,
~rQhk .
Th e prlP "1,111\(,,1 j1rpri'jn ].ldI1 t" Si lOllld tlll h tHo, u:·wd ht
lppro.;,jnllllC'
<; . /("
moit'(:ul.1I
L h~H.·111Iinl' ., "
w ~IGhl
www.invitrogen.com I7
•
'i Invitrogen '
life technologies
Most accurate estimation of molecular weight
The 12 protein ba nd s on the M,lrkl)
migrate th e closest
to
UIISlrtilll'li
SfJrllJan l
their true molec ul ar weight. That's
dard are un stain ed . th ei r migration patte rn is not mod ifi ed
by the dye. all owing yo u to achi eve th e mos t accura te
because the dye used in pre-s tained standards ca n affect
es ti ma tion o f molecu lar weight. The MiHk1 2" bands
band migra tio n pa tt ern s, res ultin g in app are nt molecula r
appea r sharp and di sti nct when vis ualized with
weights th at al-e difl'ere nt from those of patl efil S in th eir
Coo mass ie' (Figure 5) or sil ve r stain .
lI n ~ I .l ill e d
sta te. Since th e protein s in the Ma rkl 2" stan ­
Figure 5 - The Mark 12 " Unstained Standard
Protein
Approximate Molecular Weights (kDa)
Myosin
200
r-:l-gdl,KI()siJ .1se
-
97.4
Bovin(' Sl'rt lil l .1 lh1l l11 i n
66 ..l
Gl ul,llllic ci ehydroge nase
55.4
t,.J Cl.lle deh yJrugt'll dse
36 .5
CJrh oni c Jnh yclrase
21,S
Lyso zy me
14.4
I 800 9 55 6288
6
Insul in B cha in .'1. 5
IJl S1IJi I) A chai n 2.5
NII PAGE 4- t 2 ')" I:lis-'ji'i ;
Gel w / MFS sl,lllled w ilio
C UOIll.I S~It' R· 250
I
31
Tryp sin inh ibi lor
Ap roti ni n 8
11 6 .3
Ph os ph oryl.1st' B
Unstained Molecular Weight Standards
The benchmark of protein ladders
lk'nLilM,lCk I'nll L'in
I.l!j d.er~
are idea l [or estimati on
of molecu lar weight of unknown pr'o teins by SDS­
ei ther Coomass ie Brilli ant Blu e R-2S0 stain (Figure 6)
or silver stain. Standard ba nds. inc lud ing two trip le­
pol yacry lam ide gel electrophoresis. Affinit y-purifi ed
int ens it y reference band s, are in easy -to-identify
pro tein s generate sharp. intense bands without back­
incrernents for pro per band icien tijical ion
grou nd [o r accu racy.
YOLl
can vis uali ze b,ln ds using
Figure 6 - BenchMark" Protein ladder
kDJ
160 -
LW
I l()
Ino ­
11>0
so---.2Q
1 '0
[,0
7()
MJ
911
RO
7n
411
[,II
I II
·llI
lO
' ,I
IS
15
10
.~
on i~ -G I \'cine
-2.0 I~~ , Ih,,-CIYrllll'
1.!.5 '\,
"pI stJ.ltlC'd witil
1\('1 ,t,lined with
CIIOnl,I";1' R-2SIJ
Cuom,)·.. i,' R·:? 5t1
www.il1v itrogen.com I9
•
• ~ Invitrogen'
lire l echnologies
Easy, accurate western blot analysis
~1 ! g l cM.1r~
\V~S l ~l l l P , .l l t'lll
SIJ,lld arti lets YOll acc urat e­
che milum i nesce nt, I1uorescen t, o r colorime tric detec tIOn
ly estimate mo lecular weig ht directly on west er n bl o ts.
methods for your analysis. With MagicMark"; you' ll
Eac h prot ein o f the MagicMark" Stan dard co ntain s an
bypa ss steps required in conv entional methods, yet
IgG bindi ng si te.
obtain sharp bands and precise molec ular weight
YO LI
can vi suali ze MagicMark" prot ein
band s simu llaneously with you r target protein using th e
es timation o n your west em blot s.
sa me ant ibody conj ugate and protocol (Fi gure 7). Use
Figure 7 - Sharp bands of MagicMark" detected simultaneously
with target protein
.-111 1Jf) h j l-\~\Jli(
I"h. 11 il, llllll\ll' l t'llt
IJ
II l·~\I.,HI
·A lkn llllL'
pl1Cl>rh,lI<1se bJ sed
1-0.'
•.:~1
Iii!!
80
,,0
.~,\
,10
.10
!~1
tit I Alk,li ill e
p hos ph,ll']se b ~sed
Pt'll'.
kLlJ
.-------,
--- -
j21J
nil
Sil
(jQ
50
,10
-
-
'\.'~~t~flllh · c("/, ..t
Clwltliliit 11 1I'·... t t'ral
Hnrser,!llish
rwru xidd s(' ilJseJ
O(·(,'C1! I. lfl
111
20
Kit
r - - - -,
120 Ill. --
-
\Vl'slcrnBr<"l'1.c Ki I
,0
f1f'
",
,10
10
:0
-
-
Luminol ~1I0!'l1rrJtt? trom
other tllilIl1l'JI ' ( \I r~1
M ...l irMark - S I ~l )Ic1J.rrl cl ll d ,11) f','< pressf'd pm teil) (oill allli llg a VS t;'p l[ope 1.1g \\'L'H.'
sepa r.lI(,u 0 11 " NlI PACE I 12 '~o !:I i, ·Tl is Gel dlld tr,lIl~ferred 10 a lIilrtlCellul,,,,,,
nleml ·rallc. Tile hlols we le prolred lI' ill. d 1:5,000 dil ut ion of II ro U;,' .lnti- V5 !,,,m,lI\,
anl ihoLly alld (i<'I Pc leLi willi tile ill diciited wrSICfIl de tec li on Sl'S!ClnS.
1.,1I1e 1: 5 III of MJgicMark" s!alld,lfd
Lalle 2: 2 ng Qi prol ein
Fo r more information on MagicMa rk' Western Protein Standa rd ,
requ est the MagicMa rk" brochure ilt \vIVW. 111\, 11 m gl' lI .rnlll .
10
I
1 800 955 6288
Standards for Western Blots and IEF
Accurate estimation of isolectric points
The Sr.RVi\ l.iq l lin M ix IEf rVLll kel provides 9 differ­
straight bands for precise results. Unlike other IEF
E'll t pro teins w il h 13 isoforms (Figure 8) fo[- determin­
markers, the SERVA' Marker contains bromophenol
ing the is oelectric poin t (pI) of a full range of
blue and methyl red dyes, so you can visualize the
un kn ow n protei n samples
IJ1
vertical or horizontal
progress of the markers during electrophoresis.
IEF gels . Si nce the standards are salt-free, you'll get
Figure 8 - SChematic representation of SERVN IEF markers in various pH fractions
Protein
pH 3-10
Riho [wcieJse II
'>Illum1 I
3-5
3-6
4-6
5-7
-5-9
6-9
3-10
4-6
5-7
3-7
c'uh"J c
j~o t (lm l l
LeCl iu
M"llglohm
II
L1C [llgl"buli ll
l\ my tngill('OS.ld<l'!' l<J.
;\1 1Ih t'l:' +
----------------------------~--~~--------
www.invitrogen.com I1 1
Protein Standards
A standard to meet your needs At In vitroge n yo u' re s ure to fllld a s ta ndard for
isoe lec tri c po in ts. 111 addition, yo u'll save tim e
eve ry p ro tein elec trophoresis app licati o n (Ta bl e 2).
with th e s uitabl e load -and-go (onnat. Ca ll and
Eac h standard provides clear band resoluti o n so
order today.
yo u can acc urate ly es tim a te molec ular weights or
Table 2 - Choosing a protein standard
-
.
-­
-
See Blue'
Plus21
Bentl1Mark "
,MultlMal1<'
~eeBlu.l! ·
Pre-~Ined
Mal1<!2 "
B.enchMal1< "
Maglc.Mari<"
GIIL)!I
Good
Good
Good
Good
Goud
11/,\ 11/,'
11/ ,1
II/ a
ili a
n/.1
II/a
Ek:.,;11 Wl'stern Illol
Good
Good
l;ood
Good
Cond
B",II
n/,I lillllledi,l(l' n,md Idl'llII lic,ltioll
!lest I
Good
Good
*
*
*
*
Sh,Irp Band,
GtU lll
Hr,l!
Goud
Bpst!
Bes l!
Besll
Gth ld
\1W HllI1l dlhlll
Gnul l
Goorl
GOllet
n~M!
Hl'si!
IJpsl!
1'1 r.Sl llll ,lI iOIl
Com!
Good
Good
n/a
n/ "
11;:1
n/,)
G.)od
Good
Coad
Ik",tl
Coad
Good
20-1 20 k[)"
p i 3 S 10 7
SERVA" IEF
Mllri<er 3~~
Application 5 DS P,\ GE
IEl' Cel
Munit()/l\'1i!jl .lI io [1
du nn!'. Ek'ctluphores,s
~ilvt' r ~l .l il U1lg
·1 250 k Da
Type 4·250 kD"
N lllU Til l
Protem:;
Na lurdl
ProteiliS
~
10- 190 klla
B~s l !
10-220
2.5-2nO kD"
Ht.2l0Illbillall[
Natura l
Prol C' Ul S
r)role ill $
~ Da
Recomh ina nt
Pl'O teill S
RL'co lllhillZl IH
'L1t ur,iI
Protein:,
rnl1~ill :)
• H.I/hls vj'lhh' 0nlr ,lll"r ~t.ll nin!}
Description Quantity
Cat. no .
MultiMark ' Multi-Col ored St,l ndard 500
~t1
LC5725
SeeB lue Plus2 Pre-Stain ed Standard 500 pI
LC5925
500 pi
LC5625
See Blue Pre-Slained Standard Be nchMark" Pre -St ained Pro lein Ladde r 2 x 250 pi
Mark12' Unstained Standard 1 ml
Benc hMark ' Protein Ladder 2 x2 50pl
10748-010
LC5677
10747-012
Mag icMa rk" Wes tern Prote in Sta nda rd 250 pi
LC5600
SERVA IEF 3-10 Ma rker 500 pi
392 12-01
,.' I " rn1l nl; I Ht.t,'T " "t II 11 h\1.H~ Prt,'" .lin,·,J I'm'"
I., ,I, ,'," rw " t,1 h\ LlIllll~+I ;., , ~. ulx:1 lJ. I.'I\( t\V. -I I . 1",''''\\(' I 1('1 1(' thf' 1\1 " ,;")t:"l T,.. ",j
..lJrr,; IIIHhttll" (Inrllul ;~ l-thd ... 1L.~Il't;' JI~·'II~·tll~ (vt '\'$l'J.dl ~·tIIlJf.'.'~. Iluh t.~(lI1U,~ (. I .) 1(,&I\ f l'H'IIIf.I,lto tllllk .J I II~ 1111, '11"'1111 ,I 11I.I~l!ttl PI.(
,I. I'! ,- ,II.
I'f.
.,IP It!
•
ti) lnvitrogen ­
life technologies
C.orpordte hCddqll<1rtl!rs ;
1600 I oleda y
,\V("IIUC •
Corl,llaiJ. CA 9 2008 USA ' Te l: 7606037200' Fax: 760 602 6500' TO il
rrcc lei. 800 955 6288 ' r mail : lee ll .>ervicei;,nvilrogen!.Um · wwwllhitrogeTlconi
European heildquartel'i:
I""ill ugenllu ' InclHlI n"n i3USllle"S Park , 3 Founta " . Dlive • 1\1isley PA4 9RF. UK ' lei . !-44 (01 t4 I 8 14 6 100 • Fax: +4 4 101 14 I 8 14 6260 • E .Tldil CUlOleC l1 " ilwilroqen co m
II J U} IJ ttl
06.~-6<) :
10M