• Annotated SAS Output (ASO) Michael P. Meredith, David M. Lansky and Foster B. Cady The analyses for 15 data sets are done using the statistical software package SAS. annotated SAS output The focus of the <ASO) is upon specifying appropriate include problems involving multiple linear regression, polynomial regression with lack-of-fit, comparison of regression lines and analysis of covariance. treatrnPnL means is demonstrated for one-way classifications • and Lwo-way factorial treatment designs from completely randomized and randomized complete block experimental de~; i qn !::. !·-,a.\/ :i. n q E!qual <::~r-, d u.1·"! t::·qui:il J•-pp 1 i c: ,;:. t. i or-,. T1,.. c~at. rnE·n t: means are also analyzed from split-plot, repeated measures and crossover experiments. BU-866-M in the Biometrics Unit series, Cornell University, ,J i::ll..ii..I.EII'.. y, • Ithaca, NY lCJU:'! • ID. t.r:..oduct. ion.. The present form of the Annotated SAS Output CASO) has evolved from the original project aimed at illustrating common statistical methods using the statistical software package SAS <BU-664-M, BU-705-M, and BU-814-M). pa.c::ka(Je The primary goal of these annotated outputs data at the level of Statistics 602. .l.••... L lJ outside the classroom. Over the past six years there have been many people who have contributed to the ASO. students, Most of these people have been either lab instructors or undergraduate assistants involved with the conduct of Statistics 602. Suzanne Aref, This list includes Anna Angelos, Valerie Arneson, Jim Babb, Calvin Berry, Margaret Patricia Firey, Laura Gnazzo, Walter Kremers, George Legall, Maatta, Charles McCulloch, gorsch, • Patricia Nolan, Norma Phalen, Cecce~ Jon Walter Ple- Beth Snelbaker, and David Umbach . P. t~l-~.~;:,;.r.-:J._p t. Lc;m. A total of 15 data sets are employed to illustrate the application of SAS in the analysis of data. The data sets are derived from actual designed or observational experiments. The name of the data set, which appears in the Table of Contents, and a description of the type of analysis that it serves to illustrate are provided below. +I'" om f}n ,::t 1 y -::.~ j:..l:l~l ~~~.~U::lg[jJJl~~ n t <:t t Q.SI t f!. The first 10 data sets are taken t:!.Y.· !3..e:- cl.t:..<~..§..~.:i..<..:?.D. ( {1 l 1 e r. 1 <.1 n d C'" d y , :l 9 U::: ) , while references are given for the sources of the remaining five. :1.) !~.r:J.:;e!Ji..£~ ..Q£~t..iE.~ i 11u~:;tl"'i:?ttf2 s~tr.. <:.1i ght--1 :i nE~ J·-·~::gr.. f?!S!Si on for- the m<;=dn ..·- slope and intercept-slope models. 2) !:J.J::.!=!fl..:L. l2.s!.t.<;!. illus;tr·,:::..tE! multiple linear· r·egt··es;siun with twc) predictor Vdriables. Model sequences are demonstrated and par- t.ial leverage and residual plots are shown. Orthogonal polynomial • contrast coefficients are calculated for unequdlly spdced treatThese contrasts are compared with the sequential model sums of squares. -i.- 4) !~.L~f:J:. r- ..i c:...Lt.. 2::.....1.!2~~.L... J1'E.d:;_§:~ :l 1 1 us t r· C:t t ~? a mCJ d f..~ 1 s e que n c E~ whE~ n s £~\IE-:? r.. a .l straight lines may need to be fitted. • Fot.~t.~.2._J::::i~.~~.f.!J.i:;u:}QET...Jd.!~~J~ 5~\. ill us;t.1·· ,:::~t<;~ an ar..lc:tl y~> is of via a complete set of orthogonal contrasts. tt-eatm£~nt me<::tn S'; The experiment design is a one-way classification in a completely randomized design with equal replication. f:..) b.:iinpho<;vtELlLiu~a ill us;t1~ <=•.te an an.:.~l y!::;:i s CJf a 2 ;.; 2 +<:tc:tclr· i al experiment laid out in a completely randomized design with equal r·eplicatiDn. 7) Fat _.kL9.!?"25.?.~J. i . t~..LLLt..L...Jl§tt.~~~ i 1 1 us t r· ate an an ctl y s i s Df +act-- a :2 ;.: 2 orial experiment laid out in a randomized complete block design. B) EJ:.:J::! t;,~;:.tJ::LJ:~.:!.t.!::.J.J) C!.IL..JL~'.tj:ij!. pletely randomized !"1 on -cw t or· t !:;E·~t t~2.l:.':!.<E!.!llR ..... R!:LJL:~:!.:t~~ !J £.; + onE?.-·-~"~ <::t ·y <::t with unequal replication. de~ign con t1r· ;;1 ~::;.t !:; h D~i CH"IiO.tl 1-·1 O.:J c::.r·1 ii'i.l :i. 1 1 us t r at t::! an .:1 n c\ 1 y s; i ;:H- F£~ p 1r F2!:;en t f?d as:; t ii!t n a 1 y s t·H~ 11 ,::ts> <:t A set of c:clinp 1 et •::? . i 1 1 u <:; t I'" ii:i t E) 1-1 <:? j !"::> CJ f c: e 1 l mE-) an s :i n factorial experiment with unequal replication. 2 :-: ::::: ii.-i The first anal- ysis follows that presented in Allen and Cady C1982). • com-· Several ANOVA tables are considered as are the weighted and unweighted cell means (the MEANS and LSMEANS of SAS). The second approach demonstrates an analysis of unweighted means which is discussed :in Snedecor ii:ind Cochran (1980). 1 () ) ti!,;l:'i b E:~ill::!.. _J::JJ..Y.:1?.LL9.lJ.;:1.9iJ;,;.id..... J~.§:\1_<Ei :i. 1 1 us; t.l'" at t") a c:: o v i:£t r" :i. <:t t e <:it 1"1 Et 1 y s; :l ~:5 • Several ways are shown to estimate treatment means adjusted and unadjusted for the covariate. ·rhe ''c:l<!:'ts;s.;ical'' {~NCClV{.'i t.:tble is given as is a test for homogeneity of slopes. :1. l ) 1:::· u t !E:!.:L.U.......!J!~:~.!E~.!2. . J2.~~~t§~ :i. 1 1 u !:; t r· ;,:t t. <·!~ .::t n ,:t n ii:'t 1 y s; :i. !5 c1 + a 2 ;: :::; f '"' c:: t tJ r- :i i::i 1 experiment where one treatment factor is qualitative with two levels and the other treatment fii:ictor :ic quant:itii:itive with three unequally spaced levels. T~;~c; <:::qui vc:d ent analyses are presented: the first :is a model sequence approach comparing quadratic regression curves (see Allen and Cady, Unit 19>; the second is an analysis of cell means wherein appropriate single degree-offreedom orthogonal polynomial contrasts and interaction contrasts These data are taken from a larger • :i. 1.':.') J"1 Cc:)c:l·n· ;,;tl'i i::'tl H::l Cu;: {:lJJ~QJJ.nX::J:!.t:. .Lt.q......n~~L~~.'. ment. Cl p. 9'7) .. '?~5'/'., :i. J ltt<:; tx set presented d t. C) the an a 1 '!!=> i ~:; qf a !;; p 1 :i. t --u rd. t f.~;.; p E) 1 L .. · The cell means model is fitted as described in Allen and Cady (1982, p.280). The approach presented allows the simple -ii- effects to be estimated more easily than in a default model ~;pt:~c • i +:i cat i Cin. The whole-unit analysis proceeds by analyzing the sums and the split-unit analysis is accomplished after removing the whole-unit variability. Such an approach can result in substantial savings of computing dollars and a strengthening of the understanding of such experiments. The usual approach 1s presented for comparison. 1 ~:;) ~~r··<':?a §yntj"'iE!S:i..J~_Q£!~!;:2! :i 11 U)";tl·-·,:::tt~:! the anal Y~";i s of <::1. r-epE~ated meas-- ures experiment with two treatment groups of unequal numbers, and two repeated measurements upon each experimental unit. The pertinent hypothesis tests may be reduced to three twosample t-tests based upon the within subject sums or differSuch an approach tends to render the analysis more understandable than does the usual ANOVA. also presented for completeness. Brogan and Kutner I. f.!.) Li.!fii.T:!!;Jg.J.._.~!J;U,_l:L.l).!.~!J~::i The ANOVA table is These data are taken from (1980). :i. 1 1 Lt ~::; t t' ;::~. t. ;.:;, t. hE:! an a 1 y !5 :i. !:; o + a t:. wD ..... p t? I" i n d c 1'- D ~5 s; u v E~ I'" experiment with unequal numbers of experimental un1ts 1n the • two groups. The calculations for this design are exactly the same as those of the preceding Urea Synthesis Data. The distinc- tion between the two designs is in the method of treatment alloThese data are taken from Grizzle (1965). cation. :1. ~:: ) . t1.Ll.J~-~{_:j.J:d 5LJ~i:=!L.£ i 1 l us t. I"' <:t t. E~ t. h (':? an a 1 y ~;; :i. ~5 o + a t h r· f'! E! -· p t=21'" i o cl t h t·· t::~ e treatment crossover design which is balanced for first order carryover effects. The layout is in repeated Latin squares. Treatment means adjusted and unadjusted for carryover effects ii:'l I'" E! 1.:;) :i. \,/ E! 1"1 i:':'t <;; a 1··- E·! t. h E-! i ,.-· from Cochran and Cox • <;:; t. <:":t 1' ..1cl i::\ I'" d (1957, E~ I'" I·-· C! ,.-· <:;;... p. 135) . -iii- These data are t:.a~en • Intrc)duc:ti Dn Df2sct··· :i. i pt. ion In {U len F:i • tr <-:.~+ J.·/ D;::~ti:\ l ::::;2 7 (:;):~ Soymi 1 k 0.::\t:.c:\ l ·-· ''=!' 1 :1. :l Electricity Load Data c· 1 ,:j 14 l PCJtato Leafhopper Data ·") .. . .:.. ; :1. ~79 :2f.J l '?::::; Fat Digestib1lity Data :~: :1. c;s) Protein Nutrition Data :::::'-1· r·t btA!atnp pl···l Do:\ta :::::6 ~?~'? Soybean Physiological Data 4~5 ::?::::::~:. L..ymphoc:yt.E~ DE1t<::! , l c::· ·Potato Scab Data ·-· l l r:.::- ,.) 1 * Alcohol-Drug Data !:.'i8 2E!O Urea Synthesis Data 66 Hf,:'lliDgl obi woy * n Di.;·~t<:'! £::- i..J 1"1i 1 k Yi E•l d Data t34 Htl * See ,,::. description for references . • -iv- w 'I• * • TITLE a;s~~!C • '1!,T6. '"5fNIC; :11ST o\ 0 S:NIC; 1, t· v = C I ; T - l o. 1 ; xo = I; (l';f.>JT 4 II 1') I2 I'• n ?;I \ •) it-, ani f.; Annotated Computer Output, p. j\c (BMDP) :.' ) l'rints n heading on coer. pHr.:r·. l. 1-; ) • 2 •, IV \.'),> I • .J 'i ARSENIC is the observed arsenic level, :. IST is the distance from the arsenic source, ~EV is the difference between Dist and the mean distance (16.1), .) •• ~ "l .'! 1 .i :; '' ..:1ne 1.~7 OBS 1 2 '5 4 f'(,r t:•.ra<"h 1•1J~;t·r·v;d, i (Jfl • b 7 B 1 • .l4 j. 7 J 1. t. () .~ • ~ 1 r-l'r.CL P~'ti\T; VI" 10 tlEV "iJ ARSENIC DATA ,..., 'f'b(·r:•· r:i.td.emen tA <T<·Ii 1.<· '' ,J:oi.t, r.Pt. "'' l1 Pd ARGENIC whlvl. '" 11la l11s four var [;,I.JJet.:: (.\·';)); 2 ~ ~- Un:t;; ., ' 1\TA; • q l'W!C: tJI<;T AR<;E•HC; l~!rN'r print.r. t,hc· 11!111 AIWl!:Nl('. v~or'i~otd~:n xo, I~'V, 10 !JfD'T', ,J(J [)EV DIST ARSE~IC -14.1 2 3.19 l -12.1 4 3.26 l 1 l 1 1 -8.1 -6 • l -4. l -1.1 4.9 1 6.9 1 1 13.9 8 10 12 15 21 23 30 36 19.9 ~ ese ol~o 1.82 1 •0 2 1 • 8 '5 2.0') 1.34 ).79 0.66 0.30 two are uaed in model@ pre · t ar enic levels. ;:- r, ~ ~ T .\ '1. R Sf' "'If; PROC REG is a regression procedure; ~ "llEL A115~'11!C =X) OFV/'II'l'H <;~tl'~ ,, ClM -;q >:;; l'uucu!v<id:;t~reillunt.rutetlB-E: 'l'hese _tw colw:.ns are used in O:fTPUT u·.JT=NEWl ,>~t·J!CTE 1 1=fHATl OfSl9'.1H='< Sill ~B is a mean slope ~odel. model'Q L'J'i"'=L'lWEC. J9'i'l. =cJi>P[D :;r•W=S-=P"'() STU~' NT=<;To)H'S; C is an intercept slope model. ~ )11(1:)~1. Af. s E"'l C = 01 s T 1:;-= Q~ o SS 1 s <; 2; D is regression through the origin. This column is used alone 2( M•JtJEL Atl.S"'IIIC = !liST/'IIOl"oJT S!:'Hl <> SSl <;S;>; E is a mean model. in mode 1 1;1; • ,§,) "'('')cL ~PSC::N!C = Xv/1'1.-J!NT : The OUTPUT stat<•ments create new data sets called OdTPUT OUT=!\!Ewl P 0 EJ!CTE:)=Y!HI 0 ; Nl!."'Wl and NEW2. Eaoh new data set contains Pll variThis column is used alone ln model~. ables in the original data set plus the variables :>-ot:r. PLCT 0.\ T ~ = NEWt:----_ nruncd in t.he output statement. ~ PL•JT RF.Sl:.ll*YI-lAT/V;l.EF='J: ]J PLCT AP<;E:<IC*OIST='*' YHATli<;H'>T~-. ·•n• ~Notice that we have directed SAS to use the data set NEW1 for these plots. If we do not specifY a 'I PDF";: *c11 ')T= 'l) 1 l.JWcR*()!·;T= 'L '/rVF~l !l.Y; d:otu sE-t t'nr n procedure, SAS will use the most recently created data set, in this case SAS would have used NEW2. DATA OFCC"P; Plot ':Q) is a residual plot for models ~ and @. Mt:f<GE i'l!:lol1 NE~i VREI•' = 0 druws u horizont!il line at RESI!> = 0. c~sl = YHAT1 - YBARi Plot "B) prints observed, predicted, and confidence limits on the same set of axes using the symbols *, P, U and 1. PRQC ~R!NT OAT~=DECQMP; DECOMP is a new data set which is a combination, or MERGE of NEWl and NEW2. DECOMP conj) [.\" \"S~'"l!C ftitl."' "'!:51 o<;S!Lll STIF::'S SFPDEJ; TITLr ~o\T~ DECOMPOSITICN FrR A1S~NIC DATA A'~ti.LY~IS; tains all the variables in NEWl and NEW2 and the new variable. RESl is the diff~ence ~ween the mean arsenic level and the predicted arsenic level l'rom model JV or \.£). vr. -'C ~ = \ I 1;) PROC PRINT prints th£' dat.11 lecomposi tion. -1- 1» • S~~UE:f~T IAL o A~! '~ETc:!' 1.,oz~~ ~ 2 XO OED VARIAE'LF:: •:~T I··~ t or ME ~.N DF :-:-~u~;;rs SCU,~"E F VALUE FqQf;)" ?. 3::'.31:~~14 H:of-'='32~7 :;7,00 C.Q001. F\ 2.3403!'6 3:.• 7 2SQ 10 ROOT Note that using DEV instead of DIS~ as the X variable produces sequential b' s that are the same as the partials. See@). Coefficient for the mean model (see p.35). Coefficients for the mean and slope model (see pp. 39 and ~5). ~ur-~ MO n:::L E:R"O'l U T::JTAL T[S ~The option SEQB produces the sequential b's. '< S <:'':I C ANOVA: SOURCE MODEL ARSENIC=XO DEV/l~OINT P ~E~:;; ~21 SS2 CLM; e -, :; ·; ~ 1:::, 1 ~ i , DEV • MEAN ANI j:: Q(J 0.2c2::4.~== s 2 - U TOTAL stands for UNCORRECTED TOTAL. MS£" P 0.3345 o.::>2E:3 -SOUAR: ~CJ ')(P t!EA''' P-SQ The option NOINT instructs SAS not to supply an intercept] [ since XO has been included in the model. When XO is in the model and the NOINT option is used, the model SS includes the SS for the mean. ROOT MSE is the standard deviation. when NOINT is used, the reported ~ is incorrect. PARTIAL ST P aii.AMfTE;l VA RIA~LE XO 1.&2P8CO= Y -0. 0 7 b 1 ~ 1 =.;> 1 1 DEV r.~:DAP.D c:srr••:TE CF T FOI'\ HO: EPRrf' PA~A~ET~R=O :.171040 c 16112 3.51!'-4.850 (1. ~lope 0 > P.OB IT I C.OCC! 0.0013 TYFE I SS 2 ~ • ~ C 3 2 10: = R( 0) E ,&8;::~74= R(DEVIO) / L The options SSl and SS2 in the MODEL statement produce the TYPE I SS (the sequential SS) and the TYPE II SS (the partial SS). VA" I ABLE OF TYP:.. II ss I xo The P option in the MODEL statement prints each observed value, predicted value, and residual. 2 6.: 0 3 f-4 0 = R( 0 DEV) !::.;;82":7'1= R(DEVJO) DEV PR~DIC- STD ERR LOWrFq~l UPFER351 OBS ACTUAL ~ALU~ FREDirT MEAN ~EA~ 1 3~19C 3~260 ~.73P ~.574 0.2~4371 ?.074 2 1.~76 3 4 1~820 o.259352 :.261 0.215145 1~020 ~.10~ 0.19726~ 2>050 1i340 ~ o.79oooo 1.oa~ 9 0.660000 Oe54170E 10 0.300000 0.072601 o.2o39~7 o.s1e3~9 0.28180~ •.10~140 0.~63402 -.7E5212 5 6 7 SUr-' 0 F su~ 1~850 RES1DU~LS !.76~ 1.650 loq4: 0,1833~4 loc26 1.714 o.1719cE 1.317 1.24~ O.l8B3f2 0.810647 RESIDUAL ~ . . ~~ Corrected Model ss When NOINT is used, the reported n- ~s ~ncorrect. -"=Corrected Total SS where both model and total SS have been corrected for the mean. NOINT causes the SS for the mean to be included in both numerator and denominator. To calculate the correct ~' subtract the SS for the mean (n~) from the MODEL SS and TOTAL SS. 3.386 0,460075 o.6a6377 2.757 -.441020 2o56C -1.C85 2 0 371 -.OqR418 2.111 o.336C34 1o679 0.094938 1.559 -.2~27&0 1.1~2 0.11f294 C,S10615 0.~27199 ~.172 Ex. : @ ~ n;? = 26.503840 33.386414- nY: 0.7462 35. 7268oo- n? (compare to above ~) ~v :IF 'SGUAt:ED F,ESICIIOL.S '~ Should be exact~y zero. The CLM option prints the upper and lower confidence limits for a 95% confidence interval on " 9-t each observed X. Model ~ ~d~ - ·- Corrected Model SS = 1 _ RESIDUAL SS Corrected Total SS Corrected Total SS l- *___;;:(~---LL=l (Re_sid_ss_) )] [P where n is the number of observations and Corrected Total SS p is the number of variables fitted, in~lud~~c the interce?t or XO. • S!:·:iU=:··'T~!tL P!T:R<E~ c;.;!METL.:( DED VARU::L::: ~l!"' OF s:;u ~:; ES 8 R"OT ') EP c. v. I "'EAN SGUlPE 6.&13~:74 F.~P2574 2.3403(:!'. C' • F V~LL;E PRCE>I=' 23.52~ 0.0004 2 9 2 ;; 4 8 = s2 , same as @ R·SGUARE 0. 7462 .(;--Correct ~. M'J P-SQ C. 714 5 PARTIAL PAkAI>'"'TfP STANDA~D ESTII'l.\Tf: ERROR T FOR ~0: PARAMET~R=C 2.ii~6?~·e Co310720 9.01€:112 ~.2t9 o.ooo1 -4.850 0.0013 YS£ L: A~., f- The adjusted ~ is "adjusted 11 for the number of variables in the model. :.:.22:.42 ['!" 1/ARH.'?L:: NTC:'~CEC DI The NOINT option is not used, so SAS supplies an] [ intercept. The model SS does not include the SS for the mean (nj2). ® \fi.Sf.tdC o• SOURCE ~10DEL ARSENIC= DIST/P SEQB SSl SS2; ;<;T!Pt~TCS 1.!0::8 2 • & :. ~ 23 • • 0 7 ~ 1:: 1 } same sequentials as D I c:T • SLOPE MODEL :9 ~T -0.078151 PROB > ITI (SEQUENTIAL) T YF E I ')S .503840 fo882:74 z~ Lsame as @ (PARTIAL) VARI.l.DL:: C·F TYP!:.. r1 SS I NT£11 CE;> DI"lT 1 1 25.241773 6 .E.825 74 Csame as ® PRU'ICT OB'> ~CTLIAL VdLU': RtSIDU-~L 1 2 :ool90 3it260 2. 73() c .460070:: 2e~7A 3 h>l20 Z.26:i. 4 l .. 02C 2.1 o~ 7 B '• 9 t:o .... 1!) .e: c lo94P -.G91'4lf- ;<•050 1. 71'· Oo33EC::'4 1.24"" O.G~4':'::'i· 1. 0 's•'; -.29~7~( ~ ~ 6 c. O.f:SE:377 -.4410::( -1.0£-:' 1.340 :;(lOGO The parameter estimates are different from those in but the TYPE I SS are the same. same as PROC REG does not compute F tests for the TYPE I or TYPE II ss. The t tests of the parameter estimates are equivalent to F tests of the TYPE II ss. To perform the independent F tests of the TYPE I SS, form the ratio ® (TYPE I SS)/df _ df (Residual SS)/(Residual df) - FResid df 0.54170!: Oo1H';''?4 ~ 0 C'C 0 o.c7280l o.:::211'?:; :o~Jo su~ o~ RESIDU!LS SUM "~ s:uA~Er RESICUAL~ @, 8.40~ 0 4~-~~ 2.34D3tE -3- • SE)UE~!TIAL cAK~~~T~~ .04~7':-73 D! oT JC::P V.l.I\IJlE'LE: c:Tl''AT~~ =slope of line through origin (seep. 53) lFiSEUC s u ~, o' SOIJ~CE llEP c.v. model. Result: fitting a straight line through the origin. r·;:: .1 N sru:.c:. a l'o144Eli1 ::.0646P4 lQ 8.1440:41 27.:82159 35.726PQQ MSE 1.750l24 M::-A~l l.f:2f<GOC "-<:91.1!\I>E' ~CJ R-SG ~RR::l"< ~nor j sr;ur;Es = s2 , F 'I•LUE F'ROt->"' 2.1058 0.1::7'0 ~L: compare to previous models ~ E N I y-£2~ USE~. R-SGUAR[ IS • •• ' c 107.5322 NOTE: NO INTERCEPT TERM IS • A?.SENIC = DIST/NOINT; NOINT is used, so SAS does not supply the intercept, and XO is not specified in the includes SS for the mean DF M0 '.lEL U TOTAL :!J . DIST R~QEFTNEC. Such a model does not make much sense with the VARIAPLZ: OF STA"JDMlD F.R R rR PARAMETi:i\=0 1 0 .c 4f.798 C,G2870E. 1.630 D~'" (PARTIAL) TYPE II ss 1 8.1441' 41 0! ST V.6'l!APL::: T FOR HO: PARA ~1['!' E.P ESTI'HTE DI ST PFiOE > ARSENIC data set, although regression through (SEQUENTIAL) IT I TYFE I SS 0.12"7': P,l44641 the origin is useful in other circumstances. MEAN MODEL @ DEP VARIABLE: DF SOU'lCE' ER."<O'l U T·}HL 9 10 'l00T r-'SE 'JEP MEAN c.v. \:) '3.22~: bO 35.726 00 1 • c 12~ 11 1.621'000 62.1".126 ss for the mean, equal to nr = 10 * (1.628) 2 14E &N F Sr~AOE 2€:.503840 1.024773 V~LUE pqG~)I'" 2=.8€:3 G.OC07 ~Note that s 2 for this model is larger than for models reduces the estimate of cr 2 c.741P C,74ll' "·SGUAP[ ~[·J R. -SG ® and @. Fitting the slope • No INTERCEPT TFR'·' r~, u~E:~". F-SG'U~RE: IS R:::c.un;e::o. 1~t.iEI> STAMO.oG OF SSTP""T::: FRR"R P~RAMET~R=r 1 l.E:2~C•CC c.?~D121 ~.OfE PARA VAk!APLC XC ~ SUM OF ............ S"U'"'""S 26.503 4 MOC'SL <§"Q!): ARSO)IC ARSENIC "'XO /NOINT T F8R HC: Pft\lB > ITI Do80C7 -4- TYFE ISS 0 This model was run so that the predicted values, each equal to y =1. 628, could be saved in the data set ONE, to be used in the data decomposition, part G;). .,. • r!msiruiLANALYSISJ PLOT OF RES!Dl•YHATl LEGEND: A : 1 OBS, A : 2 OASt ETCo @ Residual plot for the models in Oo6 + .) ® and A @. RESID is on the vertical axis, YHAT is on the horizontal axis (see p. 317). A 0 o4 + A A Oo2 + I I l 0 .o \· R ::. s A A · ·-·--------------------·---------------·-------------------------------1 I I -o .2 /ote the horizontal line drawn by VREF = 0 A I + I D GD A u A L s OBS -0.4 + A / DATA DECO~POSITION FOR ARSENIC DATA ANALYSIS ARSENIC 1 2 3 1 o6 2fl 1o628 1o628 lo628 1.628 1o628 lo628 1.628 1o628 ~ 1.1019 0. 74 56 0.6330 0.4767 0.3204 0.0860 -0.3829 -0.5392 -1.0863 ~..-...,.-.--' lo62~ -~ (yi -i") 7 8 -o.8 9 + 10 I I I I :vi I I I A •1o2 + I I -·---------·---------+---------·---------·---------·---------·---------· 0.4 o.P 1.2 2.0 2.4 o.o 1o6 2o8 PREIHCTED VALUF - y + STORES RESIDl 0.4601 Oo6864 -o." 41 o -1.0847 -0.091l4 0.3360 .. 0.0949 -0.2988 Ooll83 1.0000 1.4461 -0.8887 -2.1538 -0.1934 0.6553 Ool873 -0.5964 ~ + (yi -yi) Each observation can be deco..!posed into the overall mean, the difference between the predicted values and the mean, and the difference between the observed and predicted values. See Unit 6, p. 50. -1.0 + I RESl 3.19 3o26 lo82 lo02 loSS 2o05 lo34 o. 79 Oo66 "56 -0.6 + YBAR -5- 0.2562 0.5671 SEPRED 0.284371 0.259352 0.215145 0.197268 0.183354 0.171956 0.188382 0.203997 0.281803 0.363402 • i LOT (,F sv~~~cL ~RS'.I,JC•l!ST CL')T (JI' YHATl+OIST fLOT OF !:FPFR •CI <:T PLOT OF LC'W":F<*CISi ' " ~ ·~ ..I I ~v~c~L SY~~~L SY~~OL u !:' u f"\ C· u u IS • IS F IS U • [' IS L ® u I • • ~~ I OVERLAY plot of the observed values (*), the predicted values (P), and the upper (U) and lower (L) 95% confidence limits for a mean predicted value. 3.0 + I ~ I 1.' I P~~ L· I~ 2 .5 + I p R E 0 I c 2.0 l<:·"~"'" 1 .s + T E 0 J 1 .a A + A L yi-yi ) I' "" J 1~ l II u L L IJ p, \.1 L + u £ o.s y =2.886-0.07815 * DIST L = + 1.628- 0.07815 Note* outside of L's 0. c + L -0.5 + '-1.0 + --·---·---·---·~--·---·---·~--·--~·---+---·---·---·---·---·---·---·---·I. ~ lC 1? ]A 1~ 1~ 20 22 24 2~ 2F ~0 ~~ ~4 3f t:: 2 ~I ~:T -6- * DEV • • • FIREFLY DATA- Units 10, 12, and 13; ACO, p. 351 (SAS) TITLE FlR=FLY JATA; DATA fiREFLY; ~~PUT fl{~E LIGHT TEMP XO=l; ~~; Creates the data ~~~ FIREFLY. FTTh1E is the ~·esponsc variable; LIGHT and TEMP are e::planetory variables. We used~~ to tell SAS to read the entire line because we have more than one observation on each line. C~RDS; 45 26 21.1 52 55 23.3 38 79 25.0 31 130 25.5 56 40 17.8 50 41 22.0 31 45 22.3 4..) 3 5 23 9 33 56 25.5 54 55 20.5 40 70 21.7 28 75 26.7 36 87 24.4 3b 100 22.3 46 100 25.5 40 110 26.7 4•J 140 26.7 0 FIREF'LY DATA OE!S 1 2 3 PRINT; VAR XO LIGHT TE~'~P FTU~E; -Prints the~:!_ matrix for@. _,--,_P~OC .!y @ ~ ~ PROC PLCT; PLOT FTI,.E*LlGHT FTIME*TEI4P LlGHJ:_*Tl;fo\P; - 4 3 separate plots, FTTh1E against each explanatory variable and LIGHT vs. TEMP. PRCC REG; MODEL FTI~E=XO LIGHT TEMP/NOINT P SSl SSZ SEQB; OUTPUT OUT=NEW1 PPEDICTED=YHATl RESIDUAL=RESIOl; MCDEL FTI~==TEMP LIGHT/P CLM SSl SS2 SEQB PARTIAL TOL; MODEL FTI~E=TEMP/P SSl SS2 SEQB; CUTPUT OUT=NEW2 PREOlCTEO=YHAf2 RESIDUAL=~ESID2; PROC REG used to i'i t reodels with both LIGHT and TEMP, and then a reduced model. 1 5 6 7 8 9 10 11 12 13 14 i5 16 17 '1\' XII 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 LTGHT TEMP 26 35 4J 41 45 55 56 55 7J 75 79 87 HJ 1 c <) 1U 21.1 23.9 17of:! 22.} 22.3 23.3 25.5 20.5 21.7 26.7 25.0 24.4 22.3 25.5 2 6. 7 25.5 26.7 13~ 140 FTIME 45 4~ 51'1 5n 31 52 3JC\ 54 4'1 28 38 36 36 46 '+~ 31 4" X ! Y matrix for the general model: ;M "'XO LIGHT TEMP /NOINT in part DATA NE~Alli MERGE ~Ewl NEwZ; PROC PLOT CATA=NEWALL; ®PLOT RESICl*YHATl/VREF=O; -Residual plot for ~and~@ PLCT RESID2*TEMP/VREF=O; -Residual plot for ®. -7- ©. • • ...-----·~I FULL HODEL FTIME = XO LIGHT TEMP/NO"-,~ @ FIREFLY DAU StOU!:NTIAL PARAMETER ESTIMATES- The sequential coefficients are the diagonal elements of t!J.e SEQB output (seep. 99). XI' LIGHT TE"''P OEP 41.352 9 = y -. See p. 94. 48,8962 -.103083 Intercept and slope coefficients for FTJNE = XO LIGHT (seep. 95). 91.4745 6.9E-Q4 -2.12753 +--Intercept and two slopes which define a tilted pla:-!e (seep. 99). VARIABL~: Note that the slope in the LIGHT direction changes sign when TEMP is aided to the model. FTIMc SUM Of MEAN SQUA«f SOURCE OF S.;)U,\"ES "'OO':L 3 14 17 29527.857 683.143 3 J 211.000 - ERP.Of{ U TOTAL ROOT t1S t:: OEP MEA"' c.v. V.~LUE F t' 1<0B>F c ,I)Q.')l 9842.619 201.710 48.795924 = s 2 Includes SS for the mean. R-SQUARE 6.985408 41.352941 16.89217 !\OJ R-SQ ~4 !t2 0 [~OTE:jNO HJERtEPT TERM IS USED. R-S~JARE IS REDEFINED. STANDARD ERROF P!IRA'~ETER ::OS Til-lATE VAr:; I Af\L E OF ,...-.. Intercept ~ xo l 91.474451 18.825103 {LIGHT 1 0.0006919891-~ u.J68339 f. TEMP 1 -2.127529~ 0.917599 ~Slope L. Partial coefficients. VA~ £ABLE xo Df 1 LIGHT t TE'-IP l rw:: T FCR HO: PARAMETER=;) 4.859 0.010 -2.319 ss PROS > ITI 45.00J 40.000 58.:))!) 50.000 31.000 52.0)0 38.000 54.000 41. O'l•) 28.000 38.000 36.0:)0 36.1)0) 46.000 6 .J.0003 0.9921 G.03t>l 7 B 9 29371.118 1152.148 = R(O,T,L) 194.42~ 0.005003143 = R(L T,O) 26.2.318~. 262.318 = R(T L,O) \ 1 2 3 TYPE I I SS \\ ACTUAL 4 5 of plane in LIGHT direction, see p. 100. Slope of plane in TEMP direction, see p. 100. 1 013$ 10 11 12 13 14 15 16 11 4').000 31.000 40.000 PREDICT 'JAUJE STO ERR PREDICT LOWER95~ MEAN UPPER95t MEAN RESIDUAL 46.602 40.651 53.632 4tt. 69 7 44.06.2 41.941 37.261 47.898 45.356 34.721 38.341 3q.623 44.100 37.292 31t.746 37.312 34.766 2.972 3.280 4.517 2. 373 2.235 2.015 3.170 2. 795 2.326 3.256 2.011 1. 843 3.231 2.259 2.821 3.478 3.847 40.227 33.616 4'3.944 39.607 39.2b8 37.620 30.462 41.904 40.36 7 27. 73 7 34.028 35.61') 37.110 32.447 28.695 29.853 26.516 52.976 -1.602 47.6135 -.65072C 63.321 4.366 49.787 5.303 413.855 -13.062 46.262 10. •)59 44.061 '). 738795 53.1:l92 6.102 51). 344 -5.356 41.705 -6.721 42.653 -.340885 43.576 -3.623 51.030 -8.100 4.2.136 a. 708 4'). 796 5.254 44.772 -6.312 43.016 5.234 \;. R(O~ \.== R(L 0) SUM OF RESIDUALS SUM OF SQUARED RESIDUALS = R(T L,O) 'Nhen LIGHT is fitted last, it does not account for much of the remaining SS. -2- 3.94351E-13 683.1429 • • ,---- • ------. FULL MODEL ~ FTIME = TEHP LIGHT SEQUENTIAL P~RAMETE~ ESTI~ATES lNTERCE:P TEMP LIGHT DED VAP. [ABLE: Note that the sequential coefficient.s from ABDO !:!re on the diagonal of the SEQB output. See p. 100. FT 1'1<0 SOUPCE OF SUM OF SQUAR':S MEAN SQUARE MODEL 2 14 16 456.739 683.143 1139.882 228.370 48.195924 POOT lolSE OEP MEAN 6,98'3408 1<.-SQUARE 41.352941 ERRCR C TOTAL c.v. PR08>f 1 same ANOVA as ~, except that F VALUE ~ ~DJ 4.680 = s2 , same as 0. 02 76 @. j the MODEL and TOTAL SS are correctei for the mean. P-SQ 16.89217 STANDARf) ERROR P~R>\"'ETER VARIABLE OF ESTIMATE lNTERC!:J> l 91.4 74451 TE~P 1 -2.127529 LIGHT l VARIABLE OF lNTERCEP TEMP 1 1 LIGHT 1 T FOP HO: PARAMETER=J PROB > ITI 4.859 -2.319 :),()JJ69198<H 18.825103 1),917599 <) • .)68 339 0.0003 0.')361 Q,!Jl(} J.9921 SS TypE I I ss TOLERANCE 2 -1)71.118 456.734 o. 0135003143 1152.148 262.318 0.005003143 0.571054 0.511054 ~ ~ TYPE I R(OI = R(L T,O) = = R(T 0) = = = R(OIT,l) R(TL,O) R(LjT,O) r all other X' s ( ~ ' '2 l\Xi- X (0, ···)' ____________, Tel = i L(Xi- x)2 ; = 1 - ~ RXi. Again, LIGHT fitted after TEMP accounts for little of the remaining SS. all other X's Same as ~. -9- • ~ PLUT OF 60 • fiREFLY DATA ~ F~!ME*LIGHT LEGEN:J: A 1 fJBS, 2 OB$ 1 ETC. B 54 51 RESIDU~L • PLCTS fTIME + I I I 57 ~ PARTIAL R=GRcSSION 21) + 15 + The option PARTIAL in the model statement frorr. @ produces this (and other) plots. These are similar to the plots on p. 141. + + A I I I ·~ + 1': + + + ' ~ 48 + 5 I I I 45 FTI ME 42 + A .) + A I + I -5 :. 39 A + + + + + + + + ... + + A A + + + + A + + + -1·J + 36 A + A I + I I 33 + 30 + -15 + A !:. -21 + I I I 27 +---------+---------+---------+---------+ -'50 0 50 100 -l 0:) !:. LIGHT + /""--.. 24 + --+----+----+----·----+----+----·----·----+----·----·----·----·----·20 30 50 60 7C BJ 90 l)J 111 121 131 141 15) 4~ LIGHT -10- LIGHT - LIGHT (INTERCEPT, TEMP) There is little linear trend in this plot, so fitting LIGHT after an intercept and TEJ.lP v1ill acc~t for a small portion of the remaining 2wo of squares, as in (.£) and @. • s~~UE~TIAL PARlMET~R ESTIMATES 41o3"'29=Y 91 • 3 216 -2.121 '14 "r- same estimates as second line, l(' T:: '1 P • REDUCL~ @ FTIME =XO TEMP /NOINT ::E; o::o VARIABLE: FTIM[ $ 0 1JRC J GC:L 2 ER~OR 15 17 M 5UM OF SGUA.RES OF ~'" u TQT AL 2'1527.852 683.148 3a211.000 R 1)0T MSE r:lEP MEAIII 6. 74P570 41.352941 16.319'14 c. v. t'.EAN SQUARE 14763.926 4 5 • 5 4 3 19 6 = s2 R-SQUAP.E ADJ R-SQ >" F VALUE PROe 324.17'1 o.J'JDt Note that s 2 has not increased very much from the s 2 in @ and @ -.-here both TEMP and LIGHT were fitted. n• .,774 0.3759 1\!0TE: NO INTERCEPT TERM IS USEDo R-SGUARF IS R"DEFINED. V~'HABLE X1 T E'•P OF P.ARAMrTER EST !MATE 1 1 31.381582 -2.1214'1'1 STANDARD ERRrR T FOR H!1! PARAMET::R=C' 5.754 -3 ol67 PllC2 ) TYF E I ITI o.o·~!'l o.il064 ss SS for reduced model (FTIME = XO/NOINT) = SS for the mean (n~) ~ 456.736 2'?q1.11E 456.734 standard errors of the estimates, part ~ OF VA"IflflLE x· 1 1 Ti:. vp IT SS TOLERANt"E 1507.671 456.734 1805.'165876 loOOODt'J TYPE F test for the need of the general model with TEMP and LIGHT over the reduced model with TEMP (pp. 138-140): difference in df between models df,general model '"~BS ACTUAL 1 45.00" 2 3 4 5 6 7 8 ;_ 5!).00~ 31.00~ 52.00~ 11 38.00~ 12 36.00~ 13 36.00~ SIJ" ) 4!Je00 ... 1'l 14 15 16 17 . 'l" ... c '16.619 -1.619 4a.679 -.b79071 53.620 4o380 5.291! 44.710 44.073 -13.073 41.952 10.048 37.285 0.715240 6.1(18 47.892 4!:J.346 -5.346 3'+.739 -6.739 38.345 -.34541l2 39.618 -3.618 4'1.()73 -8.073 8. 715 37.285 34.739 5.261 -6.2R5 37.2f\5 5.261 34.739 58.00" 38.00') 54.001 4(1.001 28.(101 9 PREDICT VALUE RESIDUAL 46.0C~ 4 o.oo, 31.00~ 40.001 '\ESIDUALS )~I..'~I)Er I'!C~!DU~L" ~.65930[-13 E:P3.147"' _(456.739-456.736)/1.!. 0 0001 (683.143)/14 - . Reduced model is adequate This test is equivalent to the t-test of the LIGHT parameter estimate in @ or @ . -11- . • PLOT OF RES!Dl•YHATI LEGEND: A 1 0~ 2 OElSo S, B ., • nc. PLOT OF RESID2•TEMP 1 O,Q + A A A A A 8 + A I I 5.0 + A + I I I 2.5 + A ·---------------------------------------------------------------------A A o.o ·---------------------------------------------------------------------A A I A -2.5 + F.: s A I I I A I I D u -7.5 + A s I I I A A -7.5 + I I • -1 -12.5 A -5.0 + A L A A -2.5 + I -s. ·; • ~.a I I R A -1 B A I ~.J oes, ETc. 7.5 + A 2.5 2 I I I 7.5 + 'i.:; LEGEND: A : I OBS, 8 A 1 ~.1 + + A I I I o.o A I + -12.5 + A -1'0\.Q + -1 '5. 0 + -17.5 @ + ® -17.5 + Residual plot for full model F'rn!E : XO TEMP LIGHT, parts @ and Residual plot for reduced model FTIME: XO TEMP, part @ @ y -2 ry.J + --·-----·-----·-----·-----·-----·-----·-----·-----·-----·-----·-----·-32 34 36 38 40 "2 44 "6 '18 50 52 5'1 -2 ~.a • ---·----·----·----·----·----·----·-15 16 17 19 18 20 rREOICTED V4LUE -12- Residuals can be plotted against or against any of t:1e explanatory variabl· 23 2'1 25 26 27 28 • • ~;---~-TA-----U-n-it_l_l-.1 DATA SOYMILK; INPUT TIME Y 00; TIME2 = TIME*TIME; LOF z TIME; CARDS; In this output the time is coded in minutes. Thus, the coefficients differ from those reported in Unit 11 by a ·factor of Eo or 60 2 for x and x 2 , respectively. 8 2.74 0 2.25 0 2.34 12 3.14 12 2.68 12 2.83 30 3.44 30 3.53 30 3.63 60 3.68 60 3.75 60 3.51 ~ PROC RES; MODEL Y TIME TIME2 I SS1 SS2 SEQB; OUTPUT OUT=QUAD PREDI~TED=YHAT RESIDUAL=RESQ; Fitting a quadratic pol::nomial as a reduced model. = The sequential and partial SS and regression coefficients are requested. ~ PROC SLM; CLASS TIME; MODEL Y TIME; ESTIMATE 'LINEAR' TIME -25.5 -13.5 4.5 34.5 I DIVISOR=2043; ESTIMATE 'QUADRATIC' TIME 415.507 -182.379 -539.207 306.079./ DIVISOR=590336.7098; CONTRAST 'LINEAR' TIME -25.5 -13.5 4.5 34.5; CONTRAST 'QUADRATIC' TIME 415.507 -182.379 -539.207 306.079; LSMEANS TIME I STDERR; OUTPUT OUT=FULL PREDICTED=YBAR RESIDUAL=RESID; = ~ ~ PROC PLOT; PLOT RESID*YBAR=TIME I VREF=0; PLOT Y*TIME='*' YHAT*TIME='P' YBAR*TIME='M' I OVERLAY; ~ PROC 6LM; CLASS LOF; MODEL Y = TIME X = [ Fitting the full (cell means) model. The ESTI~ffiTE statements compute the sequential coefficients using the orthogonal polynomial contrast coefficients obtained via the ORTHO algorithm (see X and L below). The CONTFAST statements give the SS associated with the above ESTIMATE statements. Compare wit~ the PROC REG Type I SS. The residual plot for t~e full (cell means) model and an overlay of the observed data, the fitted means and the predicted response from the quadratic model. This provides an easy -wa:: to assess the Lack-of-Fit due to fitting a lower order polynomial rather than the full (cell means) model. TIME2 LOF; ~o1 ~ 144~] --+ [~0 -13.5 -182.379 ::;~~']x) Note: _:;\ 12 1 30 900 l 60 3600 • L ,. 1 1 ORTHO 1 4. 5 -539· 207 34.5 306.079 E(x-~(0)) 2 The computations required in the ORTHO algorithm may be carried out by any regression program. For example, the last column of L may be obtained as the residuals from a regression of x 2 on x and x (see p. 109). 0 These results vrere found using the REGR command in MINITAB. Compare with L on p. 114 where time is in hours. = 2043 E(x 2 -~(0,x)) 2 = 590336.7098 -13- ••• ® • • @ PROC REG output for the fitted quadratic polynomial oodel Test for lack-of-Fit SEQUENTIAL PARAMETER ESTIMATES INTERCEP TIME TIME2 3. 12667 GENERAL LINEAR MODELS PROCEDURE =y 2. 62141 0. 019814" bl· 2. 41049 0. 051197 -5. 1%:-04 = b0·12 = bl•02 CLASS LEVEL INFORMATION = b2·0l LEVELS CLASS 4 LOF DEP VARIABLE: Y MEAN SQUARE 1.431282 0.035323 F VALUE 40.520 R-SQUARE ADJ R-SQ 0.9000 0.8778 STANDARD ERROR T FOR H0: PARAMETER=0 PROB > ITI 0.100670 2.410490 0. 051197 0.009055173 1 1 -0.000507621 0.0001412264 23.944 5.654 -3.594 0.0001 0.0003 0.0058 OF SOURCE 2 MODEL ERROR 9 11 C TOTAL ROOT MSE DEP MEAN c. v. SUM OF SQUARES 2.862563 0.317903 3.180467 0.187943 3.126667 6.010972 Partial PARAMETER VARIABLE INTERCEP TIME TIME2 OF ESTIMATE VARIABLE OF TYPE II SS INTERCEP TIME TIME2 1 1 1 20. 251745 1. 129142 0. 456351 DEPENDENT VARIABLE: Y TYPE I SS = R(x Ix,x2 ) = R(xYx0 ,x2 ) = R(x 2 jx0 ,x) 117.313 2. 406212 0. 456351 0 12 30 60 NUMBER OF OBSERVATIONS IN DATA SET = 12 PROB>F 0.0001 DF SUM OF SQUARES MEAN SQUARE F VALUE MODEL 3 2.88580000 0.96193333 26. 12 ERROR a 0.29466667 0.03683333 PR > F 11 3.18046667 R-SQUARE c. v. ROOT MSE Y MEAN 0.907351 G.1382 0.19192012 3.126666£.7 OF TYPE I SS F VALUE PR > F 1 1 1 2.40621204 0.45635131 0.02323665 65.33 12.39 0.63 0.0001 0.0078 0.4500 OF TYPE I II SS F VALUE PR ) F 0 0.00000000 0.00000000 0.02323665 0.63 0.4500 SOURCE 1 VALUES = R(x0 ) = R(XIX ) = R(x2j~0 ,x) CORRECTED TOTAL SOURCE TIME TIME2 ~OF SOURCE TIME TIME2 LOF 0 1 Note: 0.000>= The F statistic reported above could have been computed from the combined results of parts A and B as F = ~S(full model) - SS(reduced model)]/ (dffull - dfreduced) SS(due to experimental error)/ dfexpt'l error or, .,-14- -15- l 0.63 = (2.88580 - 2.862563)/(3 - 2) o. 29466667/8 • • GENERAL LINEAR MODELS PROCEDURE ~· CLASS LEVEL INF8RMATION CLASS LEVELS TIME VALUES • 0 12 30 60 4 NUMBER OF OBSERVATIONS IN DATA SET = 12 )EPENDENT VARIABLE: Y OF SUM OF SQUARES MEAN SQUARE F VALUE ~ODEL 3 2.88580000 0.96193333 26. 12 ::RROR 8 0.29466667 0. 03683333 11 3. 18046667 R-SQUARE c. v. ROOT MSE Y MEAN 21.907351 6. 1382 0.19192012 3. 12666667 DF TYPE I SS F VALUE PR > F 3 2.88580000 2:6.12 0.0002 30URCE :ORRECTED TOTAL SOURCE TIME SOURCE TIME CONTRAST OF TYPE III SS F VALUE PR > F 3 2.88580000 26.12 0.0002 ss F VALUE R(xlx 0 ) = 2. 40621204 65.33 12.39 DF. 1 1 LINEAR QUADRATIC R(x 2 jx ,x) ~ PR ESTIMATE PARAMETER=0 0.01981400 -0.00050762 8.08 -3.52 PARAMETER bl.O: 0 2.01 . T FOR H0: 0 LINEAR QUADRATIC 0.45635231 > PR 0.0001 0.0078 0.0001 0.0078 LSMEAN STD ERR LSMEAN 0 12 30 2.44333333 2.88333333 3.53333333 0. 11080513 0.11080513 0.11080513 1=171 -.:. E.4E,F,6F.F. 7 Ql_ TIME ~ ~ t7!.Pt.?!C: ~ 7 PR > F Note that s 2 is the pooled estimate of 0.0002 experimental error with 4(3-1) df. l I This is not of any real interest since it is J a test of J These SS correspond to the TYPe I SS reported in PROC REG. H0 : ~0 = ••• = ~ 60 -.... STD ERROR OF ESTIMATE JTI 0. 00245147 0.00014421 l These estimates are the same as the sequential estimates reported in PROC REG via the SEQB option. J' The standard errors differ because the experimental error is used here, whereas the residual error was used in PROC REG. l LEAST SQUARES MEANS y F ~ = s2 These are the means for each time and their standard r •71 01171 -16- errors. Note that SEM = {):036833/3 1= 0.1108 • RESID 0.40 + © PLOT OF RESID*YBAR SYMBOL IS VALUE OF TIME • PLOT OF Y*TIME PLOT OF YHAT*TIME PLOT OF YBAR*TIME SYMBOL USED IS * SYMBOL USED IS P SYMBOL USED IS M • * Residual plot for full model. J.7 + @ ~ I I 0.35 + I I ~ p 3.5 + 3.4 M * 3.6 + * * + I I p R E D 3.3 + 3.2 + I I I 3.1 + T E D 3.0 + v 2.9 + 2.a + c * p 1 I I A l u 0.00 +---------------------------------------------------------3-----------l E 2.7 I I* + I I -0.05 + I I I -0.10 + I I I 1 2.6 + -0.20 + I ------__ Sum of these squared / gives the SS due to /: Sum of these squared gives * l ss(Full)- ss(reduced) 2.5 + 3 0 = SS(I.OF) I IM 2.4 +P 6 -0. 15 + I I I l I I* 2.3 + I* I 0 1 -+---------+---------+---------+---------+---------+---------+---------+ 2.4 2.6 2.8 3.0 3.2 3.4 3.6 3.8 2.2 + -+---------+---------+---------+---------+---------+-----0 12 24 36 48 60 TIME YBAR -17- • ! ~ECTRICITY LOAD DATA·,~ 13; ACO, p. <6' (~DP)I • TITLE ELECTRICITY LGAD CATA; DATA ELECTJC; '/3 ::.ndicates that DAY is a character variable. INPUT OATE C~Y $ TE~P Yi X3=J; X4=J; X5=J; XE=); Xl=l; X2=TEMP; IF DAY='SU' cc CATE=5 THEN DO; All statements ~r~ a DO to an END are exec~~ed as a group. X5•1; X6=TEMP; Xl=ili X2=0; END; SA· is changed to Q for ,;.t:::h::e:......!p:::l::::o:..:t:......::i::;n:._b~G:,:._:·_ _ _ _ _ _ _ _ _ _ _ _ ____, IF DAY =1 SA' THEN DO; 1 1 Xl=l; X4=TEMP; Xl=O; X2=0; JAY= 1 Q 1 ; END; See ® and ® for the X variables created CARDS; by the above statements. 1 ® ::>~-,:: Pf\I"JT: "il\ ...::; ~ ]) " 001"" 0 ~ R•'O. >: Y 1 X:: VAl' .. . X 4 ':: 1 1 3 intercepts and 3 slopes. '· t;-; Prints the X matrix for the full model: O:>H:T• "' ... V!l.P. -~: 'I 3 X": T ~ r-;:: Prints the X matrix for the reduced model: GLI"l MOCEL Y=Xl X2 X3 X4 v5 XE/~OI~T PI OUTPUT CUT=~E~l !=~EDICT~Q=YHATl "'~" • "'L ~ T CAT A 3 intercepts and 1 slope. ~[f!CUhL=P~~!Ol: =•. c: i·' I: ?LOT ~E~Irl•YHATl/VIi"'!==C;} Residual plot for the full model. ~ 0 ~·~· Gu•; MC~EL Y=Xl our~ur ~R"r I 'G' PL~T X~ '15 T~VD/~CI~T; LU!=~~w: c~F~ICTL~=Y~4T~ >i[SH.Ut-L=~rsro:;:; } Fits the reduced model. ~ATA=~rw2: PLCT PESir2•Y~-'AT2/VF.'"!==Ci The residual plot for the reduced model. 0 L~.T YoTEr->F=CAYl y vs. TEMP, and the character used to plot each observation (Y) is the data stored in DAY which corresponds to that observation (i.e., the first letter of the day in which the observation was·taken). -18- • • ~ The X natrix for the reduced model in ']) The X matrix for the full model in ,]) , p. 145 /1L - : -ELECT'<IIITY --r,f('~1 _____...---~ lCtD JHA .' Y.:: >3 1 1 77 7"' c 0 0 0 4 0 5 () ~ 1 7 8 1::' 11 c 72 79 0 0 :2 14 ~ l~ : 7"' 77 77 1 ~. 0 0 17 1 :::: 1 1 1 1 1 7 f'. 7a c 2~ G 0 ::·G. 1 1 1 1 7q 73 ~s ~· ~ .: G 31 c c Xl =1 if X3 = 1 if X'5 = 1 if X2 =temp X4 =temp X6 =temp 0 75 "E 1 Q 0 :J G ~. .'i 2 1 1 0 c 1:"> ~} 4 77 0 G G f. e. ~ ·~ v c. 0 74 1 ~ c J 0 1 "u G G G 0 a 1 ;:::. b 7 & C· 0 0 71 Ci n 0 0 1 ~ 0 2 ·.:; 30 31 weekday, 0 otherwise Saturday, 0 otherwise SUnday or holiday, 0 otherwise if weekday, 0 otherwise if Saturday, 0 otherwise if Sunday or holiday, 0 otherwise ~ 1 a c 0 0 1 0 0 ~ c c 0 0 78 78 1 0 0 1 7'+ 77 0 0 79 0 0 G . 1 0 c 71 73 73 J c 7: 0 0 0 l 0 69 71 0 f:-8 ~i/ Separate Intercepts -19- 0 ~ 1 0 24 0 23 ;2 1 1 E.G ;:.:: 77 77 82. 74 72 "~ 0 !'1 7"' 23 c 0 1 0 0 77 c 7f: :7 72 79 0 1 0 c 6 0, 1 -' "- a G u 1 1 0 17 12 :9 20 r: 0 0 0 7A 0 G c 1 1 :0.6 J c c 7".: 7':' 77 0 c 74 a c " c-· c 77 G 0 0 " "·c (\ " .' 14 i5 c T ':~' F 12 13 G G 1 X5 c c j E.;< "3 JlT~ 1c 11 :, C· lJAO ~ 7 P2 -. :i. D ~ 0 0 72 71 73 :>3 ?'I 27 c 0 G 't6 0 7E 7'+ ~ 77 ~2 0 c 77. l lP J fLECTP"CITY .~ ~ Y: Q r ' 17 -~ 81 £5 g @ Separate Slopes Separate Intercepts . • t_Common Slope • D-: =:. '! o::: '' T v A" ._9) FULL !WD~L, 3 .:f: T : f L ::: Y ~E' .~.22 X2 :.:~ :·::. X5 :·:.: :miNT = 72 y s :- !_ ~ c: :-F ~·L~··; t)e rw'! :-A ~-J SGUt.RES s Q L1 ;\ 1:' ~ ·: '::L E: 34~4C~~7.0~721010 57~Cl~2.~4Se~~C2 '~". <;'( 2:: 2E:S47,'.iC27E.990 106:.7H11H~ 31 ::47cES5.CCCDGOOO :~ 'J':-J=l'1E~i!CD • :" SLOPES r··.TPL F '" ~ t! L'.l~ 4::2.:::. ~: ). F :.COOl Like PROC ?EG, PROC GLM computes R2 when NOINT is used c.v. STO DlV ~.lCl~ ~2.~4P3707~ s J Jq c c: (sequentials) TYP> I SS i'F 1 y ~·£~~- 1C':2.E:7741"i?~ F V.lLUt:. ~· R > r:: o.oc·c: \( C779.42430620 47 3'?20.COCOCOCO ?.4621.01 47.64 4431.79 0.802: X Ei>'C.OCOCGOOJ 27.C9 o.:GC·: 2""1.aocooooo ::u.t:P.E·2771 3464.41: C· .:.GGl 0.~3 '2.~7~= X 1 \( ~62 ::.t: \( \( -~ r,J •:: C f. ~~32.1QQ4761• (partials) TYPE IV SS r>F X1 F c.:CCl V A.LUE. F· R n I. > "' 4 c:. c;; 1 X2 ~ ' 613.59455571 5C77"'.4248C620 X3 1 1~3.724GG562 c.'5.8 47.64 C.lO \(4 ~ 2e~.::o.OOC00008 27 .C"' 1E:C3.8411E:G67 1 .50 ::.• 2. 5E::-.6f.1~2771 c ·~~ "" Ca47~c 1 x:: l( f, PARTIAL <>:."c\M::-Tc::~ ·~ S 1 1•'' A '!'!:. T to : ~='0P HC! R .\ r.o ': T ~.1'1: 0 Y 1 weekday intercept=11 G • "34 2 1 9 2 7 X? weekday slope = 13 • 3 0 <; 3 C2 3 3 C.76 6.'70 Satur. intercept=·to2.142E:714 'f4 Satur. slope= 13.':7142€'5.7 <:: Sunday intercept=5~:;. t: 50 E: r :< 41 ·t ~ Sunday slope = 4 .1 2 0 4 t: 1 9 ~ .. ;:: .• 31 :. -~ 1 1. 2 3 c.7? ·~: H< .., IT I • ... ...... c.:cCl ~.7'577 o.ccc: ~ 14 ~TC ER~~~ OF ~~TJV'T~ (.4551 :'-~ .ce1 C!1°S' c.ooc1 1.=2P 32'51 9 • .21C· :;t;c.7 2.£:728487 c.2314 t.::s.~4:::2;:s C.OOCl c.1:.11 (.4739 :~ '5 -~ -:::-.;- .fE6~074E: PROC GLM has no option which gives estimates of the sequential b's. • .----•!:..--~ REDUCED MODE:L, 3 INTERCEPTS AND A CCMMON SLOPE @ Gf~~P'L :=:c:::r:~·r·'...i :; '·I~ "~ -.,... · .: ~ :; E L C.: C: LI~FlR ~OOELS Y = Xl X3 X5 TEMP /NOINT PROCfDUPE y r.f su~ OF SGUH\ES ~F~N sr.UAP> F VALUE. 79%.55 ~L 4 3473e249,28002397 8E:845E:2,32CCJ5:o .:::.:-~~a 27 29315.71997603 1S85.7674:E.52 31 34767565,000COOQO ~\z~~ c.v. STD DEll y ''E.! N J.~7 3.1302 32.950%4'?1 1052.67741S35 •J'I':')~~ECTt:: S: "':'j'{CI: Sequentials TYPF. I S$ C:F (1} :< :" intercept. t:; T" r-,:.L "'P~ slo;o• 1 1 1 1 R(l) R(311) R(5j3,1) R(Tj5,3,1) = 26"24:3932.19 047619 = 4 7 2 3 9 2 0, 0 0 0 0 0 0 C0 = 3692841o800GOOOO = 77555.28954779 Partials TYP/0: IV S~ s'":ur<cF "il 1 )(3 )(5 T r ·•p 1 1 1 o ~ ·' l.'·• ··r::.;:;: • R(lj3,5, T) =1 :134.52471<:.2.0 R(3jl,5,T) =o,OOC06809 R(5,1,3,T) = 1325,61070453 R(T 1,3,5) =77555.28'?5477<! ro;TI r'A TE X: weekday int,,•cept=i52. 7367432€: Y '3 Satur. intc.·cept= 0. 0 2 9 0 34 97 Sunday inte~ · ~pt=-13 0, 4 286 C.9 3C I(= T:.:.''ol common L?pe=yl2.75552448 1 T FOR HO: P:\R.\MF.TE.f\=0 1o33 c.oo -1.1 () 8.4 5 PR ) F 0.0001 To evaluate the adequacy of the reduced model, form the F statistic [(Model ~'P F VALUE.. F c .n 01 2417 0 .£(; 4350.77 :;'.401.14 71.43 F ) C:.'R ) 1.78 0 .o 0 1 .2'2 71 .4 3 Col931 0. 0 ':'98 PF ) IT [34,740,917-34,738,249]/2,;, 1.25 = ~ 1065.916 25 0,00('1 GoC:G01 C,J001 VALLI( Based on the relatively small F value, the reduced model is judged to be sufficient and there is no need to fit separate slopes. F 0.278=! 0,0001 I c.1931 o.'?':'9P 0.:: 7&'? C.C001 difference in df model ) - (Model SS, reduced model ) ] / b t th 2 d e ween e mo e 1 \Res:i.dua.Y ss-;-full modelJ/ (Residual df, full model) ss, full ~T ERROR OF [ STIMATE 0 114.42601&14 115.945065gC 118,04118545 1.50924939 Predic;.: :>n equation given ·.n p. 146 -21- • • I PLOT OF RCSI01•YHAT1 @ ~=·~xnt LEGE~O: A • RESIIllJAL ANALYSIS = l OBS, A = 2 OPSt £TC. I LCGEND! A PLOT OF Rf.SID2•YHAT2 Residual plot for the :f'Ull model R!: SI 02 50 40 ~ A = 1 OBS, e : 2 OFSo Residual plot for the reduced model A 30 A 40 A A A A A A 20 A A A A A 30 A 20 A 10 I I I A A •--------------······-···------------------------------A-------------···· 10 I I I A 0 ·-------------------------------------------------------1---------------- -10 A A A -20 -10 + A A A •30 -20 I I A + I I •4 D + •30 + -4 0 + A A " A -50 -~ + A -50 0 A -70 -fl 0 -·-------·-------·-------·-------·-------·-------·-------·-------·------1100 900 950 1000 1050 1150 1200 ll5 0 I I I + A A A .-60 -70 + --·-------·-------·-------·-------·-------·---·---·-------·---~--·-·-----1200 11!i0 1100 800 850 900 950 1000 10~0 ~00 Yl-'i<T1 YHAT2 -22- • y 1250 PLOT OF Y•THH' • IS VALUE OF DAY 5YM~OL @ Plot of Y vs. TEMP. + F The lines are the estimated regression lines from the reduced 3-intercept, 1slope model in part ®. T 1200 + Weekdays: yw =lll8+12.76(xw -75.67) M F T 11'00 II + ,, \.1 11 0 0 + p 1 0 ~0 Saturdays: YQ = 972 + 12. 76(~ -76.2) + Q 1000 ::0'50 900 + I I I I + I I I I Sundays: +12. 76(x8 -77 .6) y 8 = 859.4 + Q s ® s B'iO + s !\CO + ---·---·-·-+·--·---·---·---+---·---·---·---·---·---·-·-+---·---·---·---·--6!! 69 70 71 72 73 71! 7':1 76 77 78 79 80 81 82 83 8'1 85 TF.MP -23- • • ,..--- .'---------,I LEAFHOPPER DATA - u:-,:.-:; 1:': TITLE LEAFHOPPER DATA; DATA LHOPPER; INPUT TRTS $ DAYS; • CARDS; \DAYS is the response variable CONTROL 2.3 CONTROL 1. 7 ~ indicates that TRTS is a non-numerical variable SUCROSE 3.6 SUCROSE 4. 0 GLUCOSE 3. 0 GLUCOSE 2.8 FRUCTOSE 2. 1 FRUCTOSE 2. 3 [The CLASS statement constructs the treatment indicator variables. CIJ_SS ic not available in PROC REG. Treatment indicators PROC GLM; \.irould have to be set up in the input statement as in the Electri::;. ty load Data or the Soybean Physiological Data. CLASS TRTS; MODEL DA YS:TRTS/NOINT SOLUTION P XPX SSI; This model, following a CLASe statement, is the equivalent of a general means model. A SO~TION option is needed after a CLASS statement so OUTPUT OUT:NEW PREDICTED:YHAT RESIDUAL:RESID; that the parameter estimates will be printed. ESTIMATE 'CONTROL VS SUGARS' } X?X prints the~·~ matrix (see pp. 179-180). TRTS 3 -1 -1 -1 /DIVISOR:3 E; ESTIMATE '6-CARBONS VS SUCROSE' ESTIMATE TRTS 0 -.5 -.5 1/E; contrasts, SAS orders levels of a classed variable alphabetically, or numerically, so ESTIMATE 'FRUCTOSE vs GLUCOSE I P• 181 the coefficient~ ~ust be ordered: fontrol, ~ructose, Qlucose, ~ucrose. TRTS 0 -1 1 0/E; MEANS TRTS; Calculates TRT means. ® PROC PLOT; @ PLOT RESID*YHAT/VREF:O; Residual plot for the. general means model in J;) PROC GLM; CLASS TRTS; MODEL DAYS:TRTS/P XPX SSI; ESTIMATE 'CONTROL VS SUGARS' TRTS 3 -1 -1 -1 /DIVISOR=3 ESTIMATE '6-CARBONS VS SUCROSE' TRTS 0 -.5 -.5 1; ESTIMATE 'FRUCTOSE VS GLUCOSE' TRTS 0 -1 1 0; LEAFHOPPER DAH The CLASS statement produces this output. rl ASS G~~ERAL LIH~~R MOCELS CL'SS LrVFL L-VELS TPTS 4 0 ~0CEDURE I~FSRM3TICN vAL ur::s C0~TROL FRUCTJSE GLUCOSE SUCR~Sr Note all'habetical ordering '-:ur-cr.:R CF OE'SEFV.t.TIU.s rr~ iJATA SFT = -:.~-- • • With a CLASS statement, SAS creates indicator (dummy) variables and actUEUly forms an X'X matrix as if indicator variables had been set up in the input statements. LEAFHOPPER DATA LEAFHOPPER OAT.\ GENERAL LIN~AR MODELS PROCEDURE GEN ER AL LINEAR MODELS PR 0 CE.O-UR F. MATRIX ELf.MtNT R~PRESENTATION THr X •X MATRIX fPENDENT VARIABLE: DAYS DEPENOENT VARIABLE: DAYS EFFECT REPRESENTATION DUMMYr01 OUMMY002 DUMMY003 TPTS CONTRI"L FRUCTOSF;: GLUCO~E SUCROSE DUMMYI')Ol DUMMY ('1)2 DUMMYnl';< DUMMYn04 DUMMY "01 DUMMY 'l02 DUMMY n()3 DUMMYI'I04 D TRTS COEFFICIENTS CONTROL FRUCT('SE RUCOSE SUCR nsF.: 1} 0.333333 ·0.333333 ·11.333333 ') (l 2 ., 0 The E option for each ESTIMATE statement prints the contrast vector (the c vector of cs) • ESTIMABLE FUNCTIONS FOR CONTROL VS SUGARS EFFECT n 0 2 Note the function of the DIVISOR= 3 option. ~STJMABLf FUNCTIONS FOR 6 CARBONS VS SUCRrSE COEF F J C1 PHS EFFECT MEAfJS TR TS CONTROL FRUCTrSE GLUCr.!:'f. SUCROSE (I TPTS ·0.5 -0.5 1 CONTROL FRUCTOSE GLUCOSE SUCROSE ESTIMABLE FUNrTIONS FOR FRUCTOSE VS GLUCOSE TRTS CONT RPL FRUCTr.SE GLUCO":;( SUCR"Sf. IJ -1 1 0 ., ? ::> ? DAYS 2.0000001')0 2.20000000 2.9(10000'.)0 3.8001JOOOtl MEANS TRTS prints the treatment means. Compare to parameter estimates on next page. COEFFICTEIIITS EFFECT N -25- • OUMMY004 1] 2 • • • GENERAL MEANS MODEL Jij Y = TRTS /NOINT GfNERAL LIN[AR MODELS PROCEDURE 1ARI'ELE: DAYS D~PE~rE~T S ~"'lCC 1-1 r,'"'t:L 4 f.3.31J00GOOJ 0~ 4 0,3"l"'J0•)0J E 63.68~oooo: E•<::: U~"JRPECTEJ TOTAL ~·~.~£ 1~.05'!0 SIJ'JR'.'O:: rF T« ~s TYP> Note order CO''TROL E~TIMA "SE =Ys VS SUG 'RS 6-CAR~ONS VS S~CRCSE F~UCT"SE VS GLUCOSE oe~::RVAT!ON 15.8450"0"" 211.?7 DEV I S3 l_3 .8"0000 J~ ·t1o96666F67 1.250COO:i~ 0,7(\00000.1 OBSERVED V! L UE .:ns !' "0"~ > !) • 0 0 "1 F 2.7250~0~" F F' R 211.27 .• ~0"! IT I STD ERROR OF ESTIM!TE 0. f): 05 Oo0003 n."' !l 01 0.19364917 0 ol 9 36 4 917 0 • 1 '? 36 4 9 1 7 0.19364917 1 D.33 llo36 14.98 l9o62 -4.32 5,27 2.56 PREDICTED VALUE > " VALUE. PR > <E--% :ilc o.~~Cl Oo0124 0.%29 ~ .3 0 "1000 0 _, o3 (F·"OPr 0 - • 2 0 ·"' : -') !' ~ c 2.30SCOOOO 2.COOJil:l0" lo70':'Ct'OOL' 2.CCO)JJ~') ~ :'i,60'!0COO~ ~.P..OO'Jj31J 4 4.0010000" 3.00;)(, 0000 :'.P':'O~J:l8D .2o~~'"'~,..o 2.c:;OO)J(IQ':' o10c'l')r"o - .lCfl"'!':'r:'G -".1t'""'l"r·c 2ofl'J'COaO':' ?.'?ro··JJ:J~· 2o10'l0000(1 ;>,20011JllilC 8 ?..30')00000 ~.2T!OJJOJO =IJ.c =l-Ie -=0 "'~~750 RESIOUftl 1 7 =J.lF 0.2236068:1 ') .23717 ')P.2]Contrasts 0.273&6128. O,'i"·6? 2 5 6 PR MEAN DAYS T FOR He: PARA"10:TER= 1 TF GLUC fl~E <;UCR F VALUE ~ 63.38~GG:JOJ (2 • IJ 0 0 0 C0 Q~ = Yc 12 .2 0 0 0 (l 0 0" =YF ) :> • 9 (:' 0 0 c t:: ': ' = YG CGNTRCL FRUCFSE SQlJAFE r-"E~N 0.27:."&6128 Sequentia1s from ABDO, p. 180 PA'<A~":TEP SGU.~RES STD 4 TQ '"S QP c.v. 95? )'• 0 SUr-< CF • .1 'J~'lonr -26- Control vs. Sugar contrast (seep. 181): Estimate= -.9667 Standard Error= .2236 = 2.0= SQ,RT[ .3333(2.2)- .3333(2.9)- .3333(3.8) 1. ~ (1+119+119+119)] o . ,.,.. • ~ Y LEA~-1-'0PPE~ GfNERAL .-- ""'I:""":::- t•-. ~. - : ,., ';~: ~ '~ HE'LE: ':" MODELS LIN~~R oqoCECUR~ D~YS SUM ~F 0~ SGUARES t'EAN SQUARI' 3 3.97500C0} 1.325010Dn >!lP.')q 4 0.3;}-:'CC~':l.l ,,,()75-~-~~~ 7 4.27:-JCOG~ R-<"~U~R: c.v. STD DEl/ DAYS "EAII! ' . "'29;25 1'.05:'0 0.27:"8612?- 2.725r'10fC" f'F TYP"" I SS 3 3.975 ::;oc c J r:~.,R:~"T::: -r~L SOVRC:::- TR TS ESTIM ~~G'RS F PR 1 7 .6 7 "•'!Oq~ 'TE PAq~METER=' -C.9E-66 667 -4.32 1.2st1o ,a) SLUCOSE 0.7090 '0') 5.27 ?.:.G OJ? SERVED V~LUE 1 2 3 A f 7 e PREDICTED V.ALUE 0~ 17.67 PR > F PR > ITI =11F =lla =lls =11 STD ERROR ')F ESTIMHE (\ .223606P~ 0.23717082 0 .273861 :?E' 0.'124 o.,-o£2 o.:'E?9 OfSIDUtL 2.000. JQOO 1.70~00000 JBO -".3o~nonr.o 3,6n··o::;ao~ 2,['00 ?- .e'J'J JOVC -~.20"'J3CCO 4.1)');-.0COOO 3,8QO JJ')O ".2CfrJO!lfO 3.C:'~(~~C~::" 2. 0 20~j:CC 'olC''9('I('O ;>.e-0"0()1)0" ?.10'1CJG0'1 ?.3')'00000 ?,Q[•(l'JO'J!) -".10~''1)('('() ?.2::('');)8(1 .r.10"~0t'r·o 2.20C:'JOCO R:i-lc F 2.3o~ooooa RESTDUALS OF SQU'RED ~ESIDUALS ~~~ OF SQU'~ED RESIDUALS ~RRCR SS 'JQ~T ORD~P AUTOCORRELATI0N ·u~·'·IN· .. ATSIJN C' ·.u~· ~u~ > VALUE ';ucROSE 0!'-<'EflVATl' F VALUE} '1,30°·:'~ _, T FOP f-10: PaC!A"':T::; CO\ITRr·L \: 6-CARI?O'i" ~"RUCT"~SE · = TRTS DAH ·J '"'EL t.A • ·~ GENERAL MEANS t-10DEL '>,;QI'\'l(){){\Q LEA~'"I-'OPPER GF.NERAL CL~SS c.1ornoooo ...... 0"~~('1':') ".:H• ""1"C" 0 -~."0')':'!:'0('0 LINEO~ a ~ss TRTS L=-VELS IJ OAT~ M02ELS L~VEL 0 ROC[DUR~ I~FORM~TIO~ V~LUES 2."6f-,f.6H7 'CUMe<R CF O'SE'VP!O>'S IN DATA SP " -27- J rC''TROL FRUCTOSE GLUCOSE SUCFI !SF' .r.2o~~~cco F. • • r LYMPHOCYTE DATA - Unit 17 TITLE LYMPHOCYTE DATA; DATA LYMPH; INPUT ATP IG V; CARDS; ® j0 no stimulation anti-IG stimulation no ATP treatment (o,o) treatment (0,1) ATP added treatment (1,0) treatment (1,1) PROC GLM; CLASSES ATP IG; Class on both ATP and IG. MODEL V = ATP IG ATP*IG/P; ~ This model fits the main effects of ATP and IG, OUTPUT OUT = NEW R=RESID P= YHAT; f and their interaction, ATP * IG. PROC GLM DATA = LYMPH; CLASSES ATP IG; MODEL Y = ATP*lG/NOlNT SOLUTION SS1; eSTIMATE 'STI~ULUS' ATP*IG 1 -1 1 -1/DIVISOR=Z; =sTIMATE 1 ATP PRESENCE' ATP*IG 1 1 -1 -l/OIVISOR=29 ESTIMATE 'INTERACTION' ATP*IG 1 -1 -1 l/DIV1SOR=2; ESTIMATE 'INTERACTION AOJ 1 ATP*IG 1 -1 -1 1; Use the general means model to estimate contrasts. Contrasts among treatment means in part ~ would be "non-estimable" because the main effects are fitted first. The CLASSES statement orders the treatments numerically: (0,0); (0,1); (1,0); (1,1). The contrast coefficients must be in this order. ------r Refers to variables in CLA.SSES Statement. 9 • I PROC PLOT; PLOT RESID*YHAT/VREF=O;- Residual plot for @ and @. -2..:- • D~ 0 ENDENT • FAC:O~LP-~ALYSIS 'l) Y = AI'P IG VA~IAELE! A~IG v SOURCE f.'F OF SGU.A.fiES SUI" MEAN SGUA~'E F VALUE "'CDEL ~ 19"6oO:'COOG0j 635.3333"33:! 87o63 ~~'lOR 4 29 .OO(' ~co,n 7.25nf)~COO PR > F CORRECTED TOT4L 7 19:"5.00t ;JCO OJ R-SQUAR[ c.v. 01 851'13 f:o335= 2o6925b24") ['·F TYP"" I SS 'l. SOURCE Q.00()4 y "TO GEV _, "E~"-' 42o500CI'!Q(~ F VALUE FR > F 39. T2 216.2 8 6.90 r."'584 o\TP 1 IG ATP•IG 1 21l8oOOOjOGO 1568.0Q')Ij(-()0 1 50.00~JOOO SOURCE rF TYPE IV SS F VALUE PR > F ATP IG 4TP•I(; 1 ia 8. o~ ~ oc. c !3 J 15f..8.0000GOllJ 1 ':'C.OOOGOOOJ 39.72 216.28 6o90 e.J0:!2 1 c • :: 0 3 2 "1 ~· ."0~1 o.~trl t'o"5e'+ C'B"ERVED VALUE PRED!CTEP VALUE RESIDUAL 1 3~+.oo:coooo 2 30.00'JLOOCO 3 62.50~00000 4 6 7 .5~ 'l(j 0000 2~ .5Qfj lj 0000 32.000JJ00!1 32.COOJ:i0')0 65.000J!lJOO 65oOOODJOO 25.CIOCJ.lll!l0 2."0C'O)Cil0 -2."00000CO -<:.5GCOCOOO 2.'50C!!oroo 25.00Qj)Q00 H.COOuilGuO •J o50t':)'J()C0 -2 .::r,r>'JOCOO 48oll00J):J00 2.:ti(aCI"C·!! 08~ERVATION 5 6 7 8 24 .so co ooco 46oOOCCOCOO 5:·.000CCOOC1 suu CF ~ESIDUALS S!JV C!= SQL'!"!EO RESIDUALS SU~ OF ct~.:T SQU~RED 9ft8E~: RESIDUALS - ERRCR SS ,Q!!T~CORPEI ATION nu<>:· rrJ-w~.ts.ow· iT ~.5cr~t~~ro r."OC~OCCO 2~.~0CO!H!CO r..~OOOOCCO eo~5'.. c·~~·O ~41~+-<;:3 Note that TYPE I and TYPE IV SS's are the same due to orthogonality. The SSI option could have been used. • • r]) G[NERAL D~PEN~E~T VARI~fLE: LIN~tR r·F ~ODELS PROCEDUR~ SUt>' ()<=" SQUtP E'3 MEAN SOUAR!'" 4r£'.9.:Jor·o.~orn "'O'>EL 4 163':&.cr.:.'JCOC1 ERROR 4 ?9.D'H.lLCO: UNCORRECTED TOTAL i' 163E'5.000J0;)01 R~~E c.v. STD DEV !).~3J E .33:-.5 2.69258241 S 0LIRCE ['f TYPE I SS AT 0 •IG 4 1635&.ooovOC:l~ PII.RA"'ETI:R a 32. C OUi)(!OC =leo 1 6!':1. 1 !l 1 1 25. STT"'ULUS A TP PRESErJCE I ~~'!';O:P ACTI0~J INTERACT ION AOJ 0 'J u0 0 0 0 = lo 1 (' 0 () 0 c c0 = ~l 0 0 0 0 0 1J 0 0 = Y11 48. - 28 • <) 0 0 0 0 0 0 0} cooooc -s.nooooooo 12. r:.c -10.00000000 7. 2 5 r 0 1 0 r: P· 197 (I F VALUE %4 =s2 .flo PR > F Y "~UN 42.5000G000 F VALUE PR > F 564 .oc ~.10('1 PR ) IT I STD ERROR OF ESTIMqE 16.81 34.14 O.OOCl 1.90394328 0.0001 1.90394328 13.13 25.21 0.00~2 '}.00!11 1.90394328 1.903'9't328 -14.71 ; .3 0 -~ s:t.~ame as for ~ 0.0001 T FOR HJ: PARAr-'ET!'"R=(I ESTIMATE ~ • Y=ATP*IG/NOINT Y SIJI!RC: ATD•!" • GENERAL MEANS MODEL .63 -2.63 lJ.OOCl\ 1.90394328} 1.90394328 o.oo3~j · e.o5?4 1.90394328 0.058~ . 3.80788655 Compare s~gnif~cance levels to those of the TYPE I SS in part ® -30- In 'A) the contrasts are computed as part of the model. In :}3) the contrasts are specified. • .----------::•---.. FAT DIGESTIBILITY DArA- ~ni: 17; ACO, p. 365 (SAS) TITLE FAT 01GtSTI6ILITV DATA; OATA FAT_CIG; [NPUT BLOCK F!T $ LECITHIN V; ) LAeEL 8LCCK = PERIGO; IF FAT='T' Af\jO LECITHIN=O ThEN HIT=l; ELSE IF F~T='C' AND LECITHII'i"'J THEN TMT=2; Creating the indicator variables. ELSE IF FAT= 1 T 1 AND LECITHIN=l THEN TMT=3; ELSE If FAT= 1 C1 AND LECITHIN=l THEN TMT=4; CARDS; T o o4.6 T 0 52.4 T 0 S3.8 c c 0 6l: •.j 0 60.1 c 0 64.4 T 1 as.o T 1 68.<; T 1 17.5 c 1 <;6.0 C 1 ~O.It c 1 98.2 PROC GLM; CLASS BLCCK T!olT; MODEL Y = BLCCK T~T/XPX; 10 OUTPUT OUT=NEW R=RESID P=YHAl; ESTIMATE •w VS WO LECITHIN' TMT .5 .5 -.5 -.5; ESTIMATE 'F~T OIFF WO LECITHIN' TMT 1 -1 0 O; ESTIMATE 1 FAT Olff W LECITHIN' TMT 0 0 1 -1; PROC PLOT; ~ PLCT RESID*YHAT/¥~Ef=O; Residual plot for ®. } ~del' Equal means/Period Indicators/Treatment Indicators and contrasts as discussed in unit 17. • • • GENERAL LINEAR ~UDELS MODEL SEQUENCE PPOCEOURE ® CLASS LEVEL INFORMATION CLASS LEVELS VALUES BLOCK 3 l 2 3 HIT 4 1 2 3 4 NUMBE~ • OF CBSERVATIONS IN DATA SET Y = Equal means IPeriods I'l'reatments 12 THE X'X MATRIX DEPENDENT VARIABLE: Y INTERCEPT BLOCK 1 12 4 4 INTERCEPT BLOCK 1 BLOCK 2 BLOCK 3 TMT 1 TMT 2 TMT 3 TMT 4 4 3 3 3 3 BLOCK 2 BLOCK 3 •4 4 I~ 0 4 4 0 I I I I I - -- -- 1 1 1 1 I) ~ 1 1 1 1 1 1 1 1 TMT 1 3 .- - , - - - - 1 l 3 0 0 0 OF SUM Of SQlJARES MEAN SQUARE MODEL 5 2729.5~083333 545.90816667 46.06 ERRCR 6 71.10833333 ll.85l38889 PR > F 11 2800.6491666 7 R-SQUARE c.v. ROOT IolSE Y MEAN IJ.<;1461C 4.7089 3.44258'•62 13.10833333 OF TYPE I SS F VALUE PR > F !~LOCK 2 TMT 3 1911.81166667 2530.72916667 8.39 71.18 O.Olfl3 0.0001 l)f TYPE 11 I SS F VALUE PR > F 2 3 198.tHl66667 8.39 2530.72916667 71.18 0.0183 0.0001 ' CORRECTED TOTAL SOURCE SOURCE f3LOCK T14T PARAMETER Contrasts: W VS WO LECITHIN FAT OIFF ~C LECITHIN FAT diFF W LECITHIN f VALUE 0.0001 ESTIMATE T FOR HO: PAR AMET ER=O PR > IT I STll ERROR UF ESTIMATE -25.78333333 -6. 5f666667 -17.73333333 -12.97 -2.34 -6.31 0.0001 0.0581 0 •.0007 1.98757716 2.81085857 2.81085857 3 ·- 1- 1 1 0 3 0 0 - - - - TMT 4 3 r-- - - - 3r·- 1 1 0 0 3 0 The XPX option on the model statement produces this listing o~ the X'X matrix. Notice that the blocks are orthogonal to one another (upper left box) and the treatments are orthogonal to one another (lower right box). Further, the blocks are orthogonal to the treatments. DEPENDENT VARIABLE: Y SOU~CE TMT 3 THT 2 -32- 1 1 0 0 0 3 I ' • FAr ~LCT JF ~ES!C~VHAT U:GENO: A • ~ ResUual ::clot :'!'c::: ~. CATA DIG~STlo!LlT~ l 2 CBS, OBS, 8 ETC. .HSIO 4 + 3 + 2 A A I I I I A + A l 0 + • I I I I A A A +------------------------------------A------------------------------1 I I I -1 + -2 + A -3 A + I I I I -4 -5 A r.. + + --+---+---+---+---+---+---+---+---·---•---+---+---+---+---+---+---+-51 S4 51 60 63 66 f9 72 7~ VH~T 78 81 84 97 ~1 ~3 96 ~~ • • • I ?::\::7-r~; :~::::·RITION TITLE: ~UTRITI l"l DATA PPOlE'If\; lf\PUT FRCTEI\ CARCS; CATA; $ DATA - C:ni;; lC I Y; H 179 h 136 I" ll:O H 227 H 217 H ll:8 :1.0 ::::crsebean observations H 108 H 124 H 143 H 140 L 309 l 22S L 1s1 l 141 l 260 L 2iJ3 l 148 12 linseed observations l lf:9 l 213 L 257 l 244 l s s s s s s s s s s s s s s 211 243 230 248 327 329 250 1<;3 211 316 14 sc~•bean observations 2l:7 199 177 158 248 PROC GL~; CLASS PRUTEIN; MGDEL Y = P~CTEIN/NOI~T SOLUTION CuTPLT UuT=NE~ P=YhAT R=RESIO; ESll~ATE 'HORSEBEAN VS OllMEAL' { ESTIMATE 'LINSEED VS SOY9EAN 1 ~ ESTIMATE •ORTHO H-8 VS OllHEAL' See p. 217 Natural r:ontrasts ~ I P SSl; PROTEIN l -. 5 - • 5 ; ---..J PROTEII\4 0 l -1; PROTEIN 13 -6 -7/0IVISOR=l3; PRCC PLCl; j) PLC T .?. ES IU* YH AT /VR. EF=O; Residual plot for Using the CLASS statement to fit the general means model, ) followed by natural an1 orthogonal contrasts, as discussed in Unit 18. See p. 219 Ortho Contrasts 'J;}. - 'O)J- • • • • GENERAL MEANS MODEL @ Y "'PROTEIN INOINT DEPENDENT VARIABLE: Y DF SUM OF SQUARES MEAN SQUARE F VALUE MOnEL 3 16839q7.~3571~29 561332.~78571~3 229.66 ERROR 33 80659.56~28571 2~~~.22922078 UNrQRRECTED TOTAL 36 1764657.0!1000000 R-SQUARE c.v. STD DEV Y MUN ~.954292 23.1656 49.1t391~66/t 213.41666667 DF TYPr I SS F VALUE 3 1683997.43571429 229.66 SOURC".: SOURCE P~'lTEIN PARAMETER PR 'lTE IN ESTIMATf. iH 160o20000000 = 218o75000000= lL 2~6.8571'+286 = Ys H L s PR > F 0.0001 PR > Ff- Tests: fl• n T FOR Hf1! PARAMETER:O PR > IT I 10.25 15.33 18.68 o.ooo1 0.0001 0.0!101 J..lH =~-~t. =J..Ls =0 not J..lH =J..LL =J..Ls =J..L 0111 ST D ERROR OF EST I MATE 15.63~03000 14.27185231 1 3 • 21316 7 7 3 Contrasts: VS OILMEAL LI ORTHO H-B VS OILMEAL HO~SEBEAN ~!SttD-VS-SOYPEAN OBSERVATION 1 2 3 q 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 -72.603571~:'1 -3.94 o.ooo~ -28.10714286 -73.681!6153R -1.~5 0.1'578 o.nno3 -4.(!1 18.41171677~ 19. 4 492 5628 In this case, the ~atural and ORTHO 1 a • 3 9 6 51 ~ 3 0~contrasts give si!lll.lar results. OBSERVED VALUE PREDICTED VALUE RESIDUAL 179.00IJOOOOO 136.00000000 161J.OOOOOOOO 227.00!10000(1 217.00000000 168.00000000 1oa.oor.ooooo 124.001100000 1'+3.00(100000 141l.OOOOOOOO 30"'.001'00000 229.00{100000 181.00000000 141.00000000 26n.oooooooo 2 03 .DODO OOiiO 1411.0000 0000 169.00000000 213. OOQO 0000 257.00000001) 160 .200!liJOO" 160.2000il000 160.2001)0000 160.200t10000 lii.P.O[Inonco -2q .2 000000 0 -0.20!100000 66.80000(100 56.fl0£100000 7 .so (1'}000 0 -52.20(1'!0(!00 -3 6. 2 0 Q0 0 (1 (I 0 160.200'J~il00 160.200:)0000 16().2(101\0000 16'l.2001):J000 160.2000,)o)()0 160.2000~000 218.750~1)0t)0 218. 750'l\l000 211lo750!l'JQOO 218.75000000 218.7500tlOIJO 218.75000000 218 .750'1i)000 218.75000000 218. 7501JO 00 218.7500\IOGO -17.20~00!!00 -20.20000000 9!'.251'1000(\0 10 .25"00000 -37.75~00000 -77.75('00000 '11.25'1'!0000 •15.75!l!lOC!'O -70.75!'l'l0000 -49.75000000 -5 • 75r"'HIOOO 3R.25000000 -35- OBSERVED VALUE PREDICTED VALUE RESIDUAL 24~ .oooooooo 271.00000000 2q3.00000000 231l.OOOOOOOO 21t8.00(100000 327.01)000000 329 .oooo 0000 251'1.00000000 193.00000000 271.00000000 316.00000000 267.00(100000 l9CI.OOIJOOOCO 177 .oooooooo 158.00000000 248.00000000 218.75000001'1 218.750!lil000 246.85711j286 246.85714286 246.857111286 246.857H286 21j6 .85711t286 246.85714286 2~6 .115714286 2~6 .85711t286 21j6.85714286 24 6.85 71'12 86 25.2511(100(10 52.251100000 -3.85714286 -16.85714286 2~6.85714286 2~6.85714286 246.85711j286 2'16.85714286 SUM OF RESIDUALS SUM OF SQU~RED RESIDUALS SUM OF SQUARED RESIDUALS - ERROR SS FIRST O~DER AUTOCORRELATION DURBIN-WATSON D 1.14211571~ 80.14285714 82.11j285714 3.1~2A571~ -53.857111286 24 .1~28571 ~ 69.1428571/j 2Clol~28571'1 -q7.fl5714286 -69.857142e6 -88 .857llj286 1eH285711j IJ.IJQn(lOOOO 8 0659 o561j 2.8571 -O.IJOl1l1001lO 0 •.~4539716 1.304P.0762 • • r s;·:A.:,lF :;::H :A':.A • I CPTIGNS lS=75 NOCATE; Sto~Afo'P; I~PUT lOC DAU TYPE TMT Y; Creates the data set SWAMP. CAllOS; Flow Chart of the Analysis PROC Glfol; CLASSES LOC TYPE; 10MODEL Y=LCC TYPE LOC*TYPE/P SS1 SS2 SS3 CLM; O~TPUT OUT=NEW P=YHAT R=RESIOl; MEANS LGC TYPE LOC*TYPE I OEPONLY; LSMEANS LOC TYPE lOC*TYPE I STOERR; 1. ':A) Model Sequence for: a. 2. PROC GUI; lOC TYPE; ClASSES MCOEL Y=LCC*TYPE/NCINT; ~ESTifiATE 1 CCNTRAST1 IN R0Wjl 1 LOC*TYPE 0 c ESliMATE 1 WEIGHTED CONT2 ROWL' LOC*TYPE ;:. ESTIMATE 1 CGNTRASTl IN ROW2 1 LOC*TYPE 0 fESTH44TE 'kEIGHTEO CONTZ ROW2 1 LOC*TYPE ~ Interac .....i on ...< s ·., ~:::>-or composite test of interaction b. residual evaluation. Decide if interaction is important. ~Interaction is not important. t~ an 1 -1 0 0 O; @ 3, ll -6 -5 0 0 J/OIVISOR=ll; 0 0 0 1 -1; 4 0 0 0 14 -8 -6/0IVISOR""l4i ' ':: ESTU4ATE 'UNWGHTED CONTZ ROW1 1 LOC*TYPE 2 -1 -1 0 0 0/t>IVISOR=Z; ~ESTIMATE 1 UNWGHTED CONT2 ROWZ' lOC*TYPE 0 0 0 2 -1 -1/DIVISOR,..2; Make 1st simple effects contrasts in each row (or col.), . . nd · ~~~~~:s~! ;hou~~!e weighted or unweighted. l © ~ cw ® ~ ~ ;w 3. Force interaction into the error term. Choose among Natural main effect contrasts. Proportional main effect contrasts 2E£ main effect contrast orthogonal to lst main effect contrast (adjusted for unequal nij ), PROC GLM: CLASSES LCC TYPE; lV MCDEL Y=LOC TYPE/SSl ·ssz; ESTIMATE 1 LOC NAT MAIN EFFECT 1 LCC 1 -1; ESTI~ATE 1 TYPE NAT ~AIN EfECT1 1 TYPE 0 1 -1; GV ESTIMATE 1 TYPE NAT MAIN EfECT2 1 TYPE 1 -.5 -.5; GD ESTIMATE 'TYPE PRO MAIN EFECT2 1 TYPE 25 -14 -11/DIVISOR=Z5; GY ESTIMATE 'TYPE ORTHO MAIN EFF2 1 TYPE 1 -.552577 -.447423; -:6- • General J.Iea.::o ;.!od.el Analysis Using Model Sequence GENERAl LINEAR MODELS PROCEDURE J) DEPENDENT VARIABLE: Y SOURC!: OF SUM OF SQUARES F VALUE 5 ;~- 42538095 2.5") MODEL ERROR 29 CORRECTED TOTAL 5.63633333 34 ROOT MSE 0.300852 6.6167 0.44085862 = = 3.99 2.68 1.56 0.0553 0.0853 0.2266 ss I F VALUE PR > F I TYPE II 1.33577182 ( 6.87 2.68 1.56 1.04276977~ 0.60773900 I \ TVPE III SS F VALUE 1 2 2 1.00971645 0.50903840 0.60173900 5.20 LOG y LSMEAN STO ERR lSMEAN 19 16 (1)6 .52631579 6.82500000 3)6.47055556 6.85000000 0.10304181 0.13075228 TYPE N y y LSMEUI P~ (35)(6.66285) 2 1553.8 STO ERR LS"'EAIII LOG 0 1 2 North Mesic Shrub @6.84000000 6.62857143 6.54545455 :0 6.86250000 11 O.Ol3d 6.60833333 6.51000000 North Mesic Shrub North Mesic Shrub N '( LSMEAN STO ERR l S"'EAN 8 6.82500000 6.46666667 6.12000000 6.90000000 6.75030000 6.90000000 6.8250COOO 6.46666667 6.12000000 6.90000000 6.75000000 6.90000000 0.15586706 0.17997978 0.19715797 0.31173412 0.15586706 0.17997978 6 5 2 8 6 1.31 1.56 PR > F 0.0302 0.2854 0.2266 Notice that the cell means agree with the cell least square means. For the General Means model the predicted values are these cell means. ANOVA (1) Source R(Mea::) R (LOC) R(TYPE ILOC) R(LOC*TYP.E!LOC,TYPE) Residual df ss 1 1 2 1553.8 o. 7749 If interaction is judged important Lo43 2 29 0.6077 5.636 If interaction is not judged to be important => both LOC and TYPE are needed (pg. 230) => @ then => @ or GV. W then @, @, or Residual Analysis: l\NOVJ.. (2) Source R (!>lear;.) R(TYPE) R (LOCI :YPE.:) R(TI~Rfl.C:ION) ?.esii'..:.sl df 1 2 1 2 0.17426467 0.11904543 0.13347658 y TYPE OJ 0 0 Near 1 () 2 0 1 Away 1 1 2 0.0853 0.2266 10 14 11 TYPE LOC OF TYPE LOC•TYPE (Inte!'action) N I LOC TYPE SOURCE > F 1 1 8176 {0. 77487218 2 ° 1.04276977 2 0.60773900 1 2 2 y LOG 6.66285714~ F VALUE OF LEAST SQUARES MEANS ME~NS Y Mr::AN TYPE I SS OF LOG TYPE LOC*TYPE [6.757 + 6.391 + 6.319./:- (unweighted average o~ estimf.~ei cell means) [6.757 + 7.172=/2 0.0536 R(Mean) SOURCE j) ~ 0 Near 1 Away c.v. lOG TYPE LOC*TYPE ~· [6"825(8) + 6.l,.c?(6) + 6o12(5)]/19- (weighted average o:' c'::ser[6.825(8) + 6"9(2)]/10 vations) PR > F 0.19435632 8.06171429 R-SQUARE SOURCE • pH UTA :iJ Not shown but the magnitude of the residuals is acceptable and no pattern is e'.'ident (one or two values s!:ould be checked). ss 1553.8 1.8176- lo3358 1.335 0.6077 = o.4818 29 -37- JV. • ..:2-- SAS • Ge!lel,::: GENEPAL LINEAR MODELS PROCEDURE .!:Cs:J.s • Ee:.:.~r.c:ion DEPENDENT VARIABLE: Y SOUPCE OF SUM Of SQUARES MEAN SQUAR!: MODEL 6 1556.2)366667 259.36727778 1334.49 ERFOP 29 5.63633333 0.19435632 PR > F UNCORRECTED TOTAL 35 1561.84000000 R-SQUARE c.v. POOT MSE Y MEAN o. '3<;6391 6.6167 0.44085862 6.66285114 OF TYPE I SS F VALUE PR > F 6 1556.20366667 1334.49 0.0001 OF TYPE I II SS F VALUE p~ 6 1556.20366667 1334.49 0.0001 SOURCE LOC*TYPE SOURCE LOC*TYPE PARAMETER ~ONTRAST1 IN ROW1 ~EIGHTEO CONTZ ROWl ~ONTRASTl IN P.OW2 ~EIGHTED CONT2 ROW2 ~NWGHTEO CONT2 ROWl ..;,tJNWGHTEO CCPH2 ROW2 ESTIMATE F VALUE 0.0001 PR > ITI STO EI<ROR Of ESTIMATE 1.30 2.52 -0.63 0.26 2.59 0.22 0.2043 o.o176 0.5336 0.7988 o.ot4a 0.8237 0.26695315 0.34666667 -0.15000000 O.J8571429 I o.53t66667 0.17500000 Interpretation: Objective: j~dged to be important (see text o.204849~ 0.23809087 0.33325779 o.zoszoa5z J ~.33369144 (1) Within the near location, community type North has a higher (0.5) pH than the average of the other two corr.muni ty types (p = • 02). (2) There is some evidence that within the near location, the pH for the Mesic Community is higher than Shrub Community (p = .20). (3) vii thin the away location, the pH for community types does not vary. -38- r. (1) to estimate the cell means from the general means model; (2) to estimate column contrasts within each row (or row contrasts within each column). > F T FOR HO: PARAMETER=O r o.sls9o9o9 Interaction is 22C and 229). • • • @ General Means Est ...... - tion (with No Inte:--c.ction) SAS GENERAL LINEAR MODELS PROCEDURE Situation: The intercction is judged to be unimportant but bath LOC and TYPE are needed in the model. Objective: To estimate main effects contrasts from the restricted m0del. The restricted model (without interacti0n) both adds the interaction SS to the error SS AND f0rces differences between rows (cols.) within allC0lumns (rows) to be equal. DEPENDENT VARIABLE: Y OF SUM OF SQUARES MEAN SQUARE "*DDEl 3 1.81764195 0.605880&5 3.01 ERROR 31 6.24407234 0.20142169 PR > F CORRECTED TOTAL 34 8.06171429 SOURCE R-SQUARE 0.225466 SOURCE LOC TYPE SOURCE LOC TYPE PARAMETER LOC NAT MAIN EFFECT TYPE NAT ~AIN EFECT1 ~TYPE NAT MAIN EFECT2 ~TYPE PRO MAIN EFECT2 &TYPE ORTHO MAIN EFF2 c.v. ROOT MSE We can see these restricted means with Means, LS Means or the P option. Community Y MEAN 0.44880028 OF TYPE l SS F VALUE 1 2 0.77487218 1.04276977 3.85 2.?9 OF TYPE II SS F VALUE l 2 1.33577182 1.04276977 6.63 2.59 -0.41500335 0.07233758 0.40174146 0.39740121 0.39793817 VALUE 0.0451 6.7359 ESTIMATE F LOC 6.66285714 PR > F PR > 6. 757 7-172 Mesic Shrub 6.391 6.806 6.319 6.734 Note that the difference between rows is the same for each column (any contrast among columns is the same for each row). 0.0589 0.0913 F 0.0150 1).0913 T FOR HO: PARAME'T ER=IJ PR > ITI -2.58 0.40 2.26 2.24 0.0150 0.6919 0.0311 0.0326 0.0324 2.24 near away North STD ERROR OF ESTIMATE 0.16115307 ~ :>.18087522 •).17791409 2 1).17766482 ').17765975 4 6.9o4 - 6.489 = o.415 6.599- 6.527 = 0.072 6.965 - (6.599 + 6.527]/2 = 0.4017 ((25)(6.965) - (14)(6.599) - (11)(6.527)]/25 = 0.397 6.965- ((.552577)(6.599) + (.447423)(6.527)] = 0.398 These main effect contrasts are contrasts among the restricte0 cell ""-"!ans. -:9- OBSERVATION • • • OBSERVED PREDICTED RES l DUAL LOWER q5:c CUI UPPER 95lf: CLM l 6.60000000 2 7.2000JOOO 3 7.200')0000 4 7.00000000 '5 6.80.JJOOOO 6 6.40000000 7 7.0000~000 8 6.40000000 q 6.80000000 10 7.00000000 11 6.20000000 12 6.20000000 13 6.40000000 14 6.2000JOOO 15 6.4000')000 16 5.20000000 17 6.20001)000 18 6.40000000 19 6.40000000 20 6.800001)00 21 7.00000000 22 6.2000!)000 23 5.60000000 24 7.20000'l00 25 7.20000000 26 7.20000000 27 6.20!)1)1)000 28 7.20000000 29 7.20000000 30 7.20'JJOOOO 3l 6.80000000 32 7.00000000 33 6.80000000 34 7.00000000 35 6.6000:)~00 6.82500000 -1). 22500001) 6.82500000 0.37500001) 6.82500000 0.37500000 6.82500000 0.17500000 6.82500000 -0.02500000 6.82500000 -0.42500000 6.82500000 0.17500000 6.82500000 -0.42500000 6.'t6666667 0.33333333 6.46666667 0.'53333333 6.46666667 -0.26666667 6.46666667 -').26666667 6.46666667 -0.06666667 6.46666667 -0.26666667 6.12000000 0.28000000 6.12000000 -0.92000000 6.12000000 o.o8oooooa 6.12000000 0.280001)0·:) 6.12000000 0.28000001) 6.9000001)0 -0.10010000 6.90000000 0.10000000 6.75000000 -0.55000000 6.75'100000 -1.15000000 6.75000000 0.45000000 6.7500000') 0.45000000 6.75000000 ').45000000 6.75000000 -0.55000000 6.75000000 0.45000000 6.75000000 1).45000000 6. 90000001) 0.30000000 6.90000000 -0.10000000 6.90000001) 0.1000000() 6.90000000 -0.10000000 6.90000000 0.10000000 6.90000000 -0.30000000 6.50621858 7.14378142 6.50621858 7.14378142 6.50621858 7.14378142 6.50621858 7.14378142 6.50621858 7.14378142 6.50621858 7.14378142 6.50621858 7.14378142 6.50621858 7.14378142 6.09856959 6.83476374 6.09856959 6.83476374 6.09856959 6.83476374 6.09856959 6.83476314 6.09856959 6.83476374 6.09856959 6.83476314 5. 71676985 6.52323015 5. 71676985 6.52323015 5. 716 76985 6.52323015 5.71676985 6.52323015 5. 71676985 6.52323015 6.26243716 7.53756284 6.26243716 7.53156284 6.43121R58 7.06878142 6.4H21858 7.06878142 6.4.H21858 7.0667tH42 6.43121858 7.06878142 6.43121858 7.06878142 6.43121858 7.06878142 6.43121858 7.06878142 6.43121858 7.06878142 6.53190292 7.26809708 6.53190292 7.26809708 6. 53190292 7.26809708 6.531()0292 7.26809708 6.53190292 7.26809708 6.531<J0292 7.26809708 SUM OF RESIDUALS SUM OF SQUARED RESIDUALS SUM OF SQUARED RESIDUALS - ERROR PRESS STAll STIC FIRST ORDER AUTOCORRELATION OURBIN-kATSON D ss 0.00000000 5. 63633333 0.00000001) 7.80612245 -0.02583880 2.02672788 +-- ~ • • SWAMP DATA - Unweighted Analysis of Cell Means -- -see Snedecor & Cochran, 7 ed., p. 418 OATA SwAMP; INPUT LOC TYPE TMT Y; CARDS; ~ PKUC ANOVA; CLASS TMT; MODEL Y -= TMT; MEANS TMT I DEPONLY; -::;:'\ The six treatment combinations are indicated in the CLASS variable ThlT. · -6' The residual !-13 is an estimate of cr 2 • PRUC SOKT; BY TYPE LOG; ~ !;. /,:\ The six cell means are computed and placed in the data set NEW. PROC MEANS MEAN NGPRINT; oY TYPE LUC; OUTPUT OUT=NEW MEAN=MY; VAR Y; -.ii.J PROC ANOVA; CLASS TYPE LuC; MUUEL MY -= LOC TY~E LUC*TYPE; MEANS LOC TYPE LOC*TYPE I DEPONLY; ·JD The main effect SS and interaction SS are determined at this step. -41- • • j[ • • One-way ANOVA on the six treatment combinations. ANALYSIS OF VARIANCE PROGEDURE CLASS INFJRMATluN LEV~L VALut:~ LEVELS CLASS TMT 2 6 j 4 '> 6 NUMBER Of OdSERVATlDNS IN DATA SET = 35 DEPENDENT V~RIABLE: SOURCE Y OF SUM OF SI.JUARES 1'1EAI'< Sl.iUAt<.E: f MODEL 5 2.42538095 0.4tJ.5076U ERROR 29 5.63o333.H I Ool'1'1-.h632] CORRECTEu TuTAL R-SQUARE c.v. 0.300852 SOURCE TMT Y MEAN 6.6167 0.440851362 6.6o2tJ51l4 OF AN0VA SS 5 2.42538095 VALUE F I'R 2.50 i)j.; > f Note: s2 is all that is to be used from this ANOVA table along with the associated error df = 29 . o.053o Dt:V STD 2.50 = s2 8.06171429 34 1/'ALU( > f 0.0536 MEANS are the cell means TI'1T N y l 2 3 8 6.82500000 6.46oo6667 6.12000000 6.90000000 6.75000000 6.90000000 6 5 2 4 5 6 Calculate: 1 ~ 1 d 6 (1 1 1 1 1 1\ = 2ffi )3"+0'+5+2+-s+"b; = 0.21389. Note: Thus, ~ -42- = 4.675 max(nij) . 8 SJ.nce i ( ) =2 > 2 m n nij the analysis of unweighted cell means is of dubious worth. • :!, 'l'here is no output from ANALYSIS OF VARIANCE J: PROCEOU~E • • DEPENDENT VARIABLE: MY SOURCE uf SUM OF S~UARES MEAN S-1\JAr<.i: MODEL 5 0.47950231 0.095'10046 ERROK. 0 O.JOJJOOOO O.OOOO.JOOJ CORRECT!:!) TOTAL 5 v.H9S0231 ANALYSIS Of VARIANCE PROCEDURE CLASS LEVEL CLASS R-SQUARE c.v. STu OEV 1.000000 0.00·)0 O.OOOOJ·JOO lilY MEAN LOC TYPE TYPE*LOC l)f ANOVA SS 1 2 2 0.21596713 0.13235093 0.13118426 LEVELS 3 1 2 3 LCC 2 l 2 6.6602171d F VALUE PK > 08SERVAfiO~S TYP~LOC ERROR F.05 1,29 F.Ol 1,29 df ss MS "!:, 1 2 2 29 1.00965 0.151874 0.61329 5.63633 1.00965 0.30937 0.3o664 0.19436 5-19 1.59 1.58 = 4.18 = 7.60 . F.05 2,29 F.25 . (N JATA SET n where: LOC SS = 4.675(0.21596713) = 1.00965 TYPE SS = 4.675(0.132:;093) = 0.61874 TYPE*LOC SS = 4.675(0.1311E426) = 0.61329 Error SS from part :!) These are only approximate F ratios because given equal weight in this analysis. · -43- t~e cell means are Loc 1 =Near 2 =Away 3 =Shrub =o the number of cell means. These are the desired SS which need to be multiplied by nh = 4. 675, the harmonic mean number of observations per cell. = 3-33 2,29 = 1.45 2 =Mesic f ANOVA of Unweigl1ted Cell Means Source LOC TYPE Type 1 = North VALUES TYPE NUMBEK OF SOURCE lNFO~MATJON • • :K) continued • ANALYSIS OF VARIANCE PKOCEDURE MEANS N 1 2 I TYPE 3 TYPE LOC l 1 1 2 2 3 2 I I MY I \ .. ,. These are unweighted means, e.g. 6.86250000 6.60833333 6.51000000· .. ' 6.471 1 = 316.825 + 6.467 + 6.120) . Thus, they correspond to the LSMEANS from the model which incluoes LOC, TYPE and LO~TYPE. Note: The N are not appropriate on this page. /'. MY 6.8.2500000 6.90000000 6.46666667 6.75000000 6.12000000 6.90000000) l 2 1 2 The standard error of any location ' 6.47055556 6.85000000 J 1 2 3 MY LOC mean~is The standard error of any type mean is / .J These are the six cell means -- compare with part 0). - They are in different order because we list TYPE LOC here and LOC TYPE in~. s2 3(~) 0.1~436 3(4. 75) 0.19436 0.1442 2(4.675) = 0.1177 Seep. 419 of Snedecor and Cochran (7 ed.) for a discussion of calculating the correct standard errors of comparisons among row means, column means and individual cell means. -44- • r-------~----·~ -~------------------.I SOYBEAN DATA- 1..::c.i"'; 19; ACO, p. 273 (J.liNITAB) DATA SOY8EA~; TITLE SOY~EAN CATA; I~P~T LIG~T $ • Create the data set SOYBEAN. YIELD ~EIG~T ~~; fJ"'V=I-F:lGHT; X1 = ((L!GHT='L');r.e~e?re L I GHT = 1 C 1 I ; ) Th "1 og~~a · 1 ~· f" saemens. t t t If th esaemen t t t ~ns~e . . d th e:;;r,ren th es~s~s . . t rue, th en th eparen. th es~s . h esavaueor; l ' 1 XZ= Xl = 1 _ x1 _ xz;' ~f ~t ~s false ~t has a value of zero. c c CA~DS; c 52 12.4 50 12.4 L 63 16.6 l 4J 15.8 l <;Z 15.9 s 52 9.1 s 66 10.2 48 12.2 61 12.7 c 51 12.3 L 5) 15.8 L 4'il 15.8 s 62 9.8 s 5'5 9.4 c c 42 ll.<J 33 11.4 L 50 15.8 l 50 16.() s 52 9.5 s 67 10.3 s 56 9.3 c c 35 ll.3 c 4d 12.3 L 63 16.5 L 49 15.8 s 54 9.5 s 55 9.5 -Sets mea.t'l of DEV @PROC STANCARO MEAN=O; = 0. C 40 llo B c 4812.1 c 51 12.2 c 56 12.6 L 33 15.·) L 38 15.4 L 35 ts.v L '51) 16.2 s 58 9.6 s 45 tl.8 s 40 8.5 s 41 8.6 c oo c o5 L 45 L 62 s 57 s 67 u.1 13.2 15.6 16. 1 9.5 10.4 I t is equivalent to Height- Height. VAR OEV; 'ID PROC Prints the ~ matrix plus height and Y (Yield) :'or PRl~T; "C". VAR X1 X2 X3 DEV HEIGHT YIELD; f0PROC REG; MODEL YIELO=Xl X2 X3 OEVINOINT SEQB SS1 SS2; CLTPLT OLT=TRY P=Y~AT R=RESlO; C_VS_TRT: TEST Xl-.5*X2-.5*X3=0; L_VS_S : TEST X2-X3=0i @ PROC PLOT; PLOT RESID*YHAT=LlGHT/VREF=O; PLCT YlELD*HEIGHT=LIGHT; Model fitting the cevariate deviations last. Use TEST statements with PROC REG to test contrasts. Instead of specifying the coefficients as with EST~~TE, use the equation that represents the contrasts under the null hypothesis. Residual plot for'~· Plot o~ the response variable vs. the covariate. @PROC GLM; CLASS LIGHT; MODEL VIELD=LIGHT HEIGHT LIGHT*HEIGHT; Model fitting sepa-a~e slopes ~~ter a common slope. 'l)P~OC GLI'!; CLASS LIGHT; ~OCEL YlELC=LIGHT H~IGHT; L SME llNS Ll GHT IS TDER.fl. i + - Adjusted treatment means. MEA,_S LIGHT; ESTIMATE •CilNTRCL \IS TMT' HEIGHT J LIGHT Z -1 ESTIMATE 'LIGHT \IS SHADE' LIGHT 0 l -1; ESTT~ATE 1 CO~MCN SLOPE' HEIGHT 1; -li01'/ISOR=2; 'Jnadjuste:i trest~cnt means AND means of the covariate (Height) in each :!.eve::. of light. • • • SUYIJEAN DATA UI3S 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 21 28 29 30 31 32 33 34 35 36 31 38 39 41) 41 42 43 44 45 ~ Control treatment INDicator X1 X2 X3 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 1 1 1 ;) 0 0 I) 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 1 1 1 1 1 fJ 1 1 1 1 1 1 1 0 0 0 0 () 0 0 0 I} 0 ') 0 () 0 ') 0 0 I] a 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 1 1 'J 1 1 1 0 1. 0 0 HEIGHT UEV -3.2 o.8 -9.2 -16.2 -11.2 -3.2 8.a 9.6 -1.2 -18.2 -3.2 -0.2 4.8 13.8 -0.2 11.8 -1.2 11.8 -18.2 -13.2 -6.2 -1.2 -3.2 -1.2 -2.2 -16.2 -1.2 10.8 -2.2 0.8 o.a 2.8 6 .. 8 ":'6.2 . 5.8 ' 10.8 o.a 15.8 3.8 -11.2 -10.2 15.8 3.8 14.8 4.8 J Light \ Shade treatment INDicator treatment INDicator ' -46- 4U 52 42 35 40 4fl 60 61 50 33 46 51 5o 65 51 63 50 63 I \ 33 38 45 50 48 50 49 35 50 62 49 52 52 54 58 I YIELD 12.2 12.4 11.9 11.3 11.8 12.1 13.1 12.7 12.4 11.4 12.3 12.2 12.6 13.2 12.3 16.b 15.8 16.5 15.0 1.5 .4 1'5.6 15.8 ·~~ 1i5.8 : 1'6. 0 ' 15. ai 15.0 16.2 16.7 15.8 1'>.9 9.5 9.5 9.6 a.a 45 57 62 52 67 55 4J 41 67 <~.5 <).8 9.1 1=1. 3 9.5 8.5 8.6 10.4 9.4 10.2 9.3 55 66 56 Height - Height (deviation of the covariate from its mean) - @ The ~ matrix plus Height and Yield for part@ • ....... _j • s•GUC:'ITIAL P~~!~ET£R 1 2 • 26 = Yc 12 .26 1• X3 12.26 1~o&E' 12.3 69 15.780€: DEP VARIAELE: .a f = .YL 9o4E-6E'7 =Ys 9.23709 .0583E8:0=canmon slope oF MEAN DF SQUARES SQU~QE 1-lODEL 4 41 45 7383.377 0.683301 lf-45.844 0.016!'o66 su~ ROOT MSE [lEP ME:A•! c .v. NOTE: NO VARIABLE Xl X2 X3 DEV VI\RIA8LE X1 X2 X3 DEV Vol.LUF. F TER~ ¥9 D-SQUAPE ~OJ P-SQ 0.129C% 12 .52':<-':189 1.03039 0/{9 IS USED. R-SGUARE IS R~DEFINEC. T FOR nO: STA~JoAr<o i::STI~"-TE ERR"R PARAME.ER=O 1 12.368"'54 1 15o9BOE28 9o237085 1 1 slope=O. 0 58368 Co0335"'2 0.033650 0.034469 OoOC22315l3 3f8.213 474.906 267.9£4 26.1Sf. OF TYPE. II SS 1 1 1 1 2259.578 3758.754 119fo866 11.402032 NUMERnoP.: JENOM~t~o.TCR: TEST: L_Vs_s )~'" 0.0('01 110155 oR12 P -'RAM""TER TF.'ST: C_VS_TRT PR 0'- 7384.~60 INTERCEPT OF ~The first three partials are the adjusted treatment means (see P• 237). YI(Lu SOIJRCE E:RROR u TOTAL I YIELD= Treatment indicators covariate deviations E"~Tll"ATfS Xl X2 DEV • GENERAL MEANS MODEL :g) NUM~RqOR: ilUJCM•tJATCR: PROE ) D~: ~ D~=': '+1 c VALUE: Ppcc )F : 315.f:04 .OH6f:'59 ')F : 1 41 "' vaLu(: rpop )I'" ~~: I OoOOOl OoOOOl GoOGOl O.OCOl 0.5623!'8 .01£:6659 ' IT TYF E. ss 2254.614 3773.094 1::'44.2f-7 11.402032 33.7431 c.ooo1 8°37.1324 : I n.oool Yc ( adj) = 12. 37 YL ( adj) = 15. 98 Ys (adj) = 9.24 TESTS of contrasts These contrasts test differences between adjusted means. The ESTIMATE option in GLM would provide the estimate and standard error (see ]) ) . -L7_ • • ]) rL-~T GF SY~BDL YlfL:·~~!G~T IS VALUl :F YT""LD See plot, p. 17 236 + I I I II CX.,Y,) I H + 15 . --L L~ /x,YL,ad) L ---.,. L~L!'- L----- Supplemental Light ~L-q-c- ' 'I L 1 • • LI~~T I L I I I I I I I 14 I + I I I I 1~ + - I 12 1 ~c l_.c c ____-c ----- r I I I i ,~,-- ~ c~J:.-- (X, ,y, • I I I 11 il " II <' II • C • ad J ) - r I I: I 11 I II I I s-- :I I 10 1l + I II Control I1I J I It --- __.- c( I' I I 11 x, y, lrl" " (' -- C I I I" IJ s shading s sis I I 9 + I I I I I I I 8 I + I I'I II I II !--·---··--·---·---·---·---·---·-~!~--·~--·---·1--·---·---·---·---·---·-:-: 3~ ~:; 41 4::'- 4!: 47~4S'Xc!:lx5::'- 5!:Xs57 5" €-1 €<: 65 67 :q rlE! Go-JT 'J 'J T :-:: r- :-- ( rl I:·~!~ (Covariate) ·-~~- • • • snvr.--AN DATA G~~EPAL LJN~AR CLESS CLA L~V~L ~OCELS l~FCRM~TION Lc-v;::Ls ~~ Liff<T ~ Treatments/common slope/separate slopes medel ~~OCE~URF VALU::S :!- ·: L S hUMPER 0F 0PSERVATICNS I~ J~TA SFT : 4~ SOYBEAN DATA GENERAL DEPENDENT VARIAELE: SOURCE LIN~aR ~ODELS P~OCEDURE YT~LD MF SUr-' OF SGU~RE:; ~~EAN SGUARf F VALUE. 4110.01 MOnEL !5 ::'19.6657E021 6::'.93::'1=1S04 ERROR 39 0.601:66423 0.01~5:;54? CORRECTED TOTAL 44 320.27244'144 R-%lUARE c.v. STIJ Dt:V YIELt' VEAN o.93.q1o6 0.99"'5 0.1247216& 12 .521<8!'!'P'? SOURCE !"F L TGHT HEIGHT 2 1 HEIG~T•LIGHT 2 SOURCE LISHT Hr::IGHT Hr::IGHT•LIGHT r:>F <. 1 ;: F VALUE FR > F 308.18711111 11.40203184 0 .OHIS~726 9906.05 732.99 2.46 c.ooo1 c.c9e: ss F VALUE' FP > c• 0 01 (·. C::P:'- TYP"' IV 10.53C<.771 11.47CE494 O.OHE:::'72 --?- > F 0.0001 ss TYP'" I P~ 338.47 7 3 7.41 2o4f. G.CCCl F :J. 0(11 this line tests H0 : t~1 "' t~2 , t~ 3 ( =tl) vs. Ha : different slopes needed. • • Sl.iYdEAN i.lATA GE~ERAL LINEAR MODELS PRULEuJ~E L • Treatments/cor!'lnon slope model OEPENuENT VARIABLE: YIELD JF SUM OF Sl.it.JARES MEAN 5-iuA"-E: MOllEl 3 319.58914296 lJtJ.)2'oill .. 32 ERROR 41 0.68330149 0.016;)6:)3;; CORRECTED TOTAL 44 320.27244444 R-SQUAk£ c.v. STO IJEY YIELiJ "'EAN 0.997866 1.0304 0.12909644 12.52b8odo~ Of TYPE I SS 2 1 308.18711111 11.4020318ft. 9246.C.4 684.15 o.ooo1 O.J001 OF- TYPE IV SS F VALUE ?K 2 1 311.810961rd9 lloit-0203184 9534.71 684.15 O.OJ01 0.0001 SOURCE SOURCE LluHT HEIGHT = R(Lj!-L) = R(l3 L,c:) SOURCE LIGHT HEIGHT = R(LIS,!l) PARAMETER CONTROL liS TMT (1) LIGHT VS SHADE (2) COMMON SLOPE (3) f VALUE o?':l2.0o I"K > r 0.0001 F VALUE l-'r\ > > f R(~l!!) a common slope before the treatments. F 1 fOR HO: PARAMETER=O PR > IT I STO f:I\KOk Uf ESTIMATE -o. 23990239 6.74354249 o. 05836!U9 -5.81 137.b1 26.16 0.0001 u.OOJ1 0.0001 J.04129927 o.u .. 9J0394 0.00223151 E5TIMATE MEANS = unadjusted treatment means LIGHT c s L N YIELD ;;::I.:;tiT 15 15 15 12.2oOOuOJ 15. 86000·)1) 9.46o6667 49.3333331 4J.lJ.B3B 5'>.1333J;; = R(L,Sju) -R(LjS,!J.) = 319. 58914 - 317. 81096 = 1.77818 = SS due to fitting (1) and (2) are the treatment means in the TESTs of (3) is the estimate covariate. contrasts among adjusted fsame as the contrasts used part ,9 ) . of the common slope of the Note that the F-value output for (2) in PROC REG is not correct. LEAST SQUARES MEANS= adjusted treatment means and their standard errors. LIGHT c L s LSMEAN STu ERR LS11EAN PROB > ITI HO:LSMEAN=·) 12,;3o89540 15. 9d0627b 9.2370851 o.o33591o O.OH6501 0.0344688 0.0001 0.0001 0.0001 YIELD Compare the MEANS and LSMEANS with the SEQB output of PFOC REG in ;f) . -50- • • • POTATO SCA::> DA:A - Comparison of regression lines in a 2 X 3 factorial - - - - - - - experiment (two qualitative levels X three quantitative levels). P. 97, Cochran and Cox, 1957. DATA SCAB; INPUT YIELD TMT S LEVEL XO XOl X02 Xl Xll Xl2 X2 X2l X22; CARDS; }; PROC PRINT N; b PROC RFG; c' PR~C '-C./ ~ODEL @ YIELD = XO XOl X02 Xl Xl1 X12 X2 X21 X22 I NOINT P SEQB SS1 CLM; and @ correspond to fitting a sequence of regression models REG; ~ODEL YIELD = X01 X02 X1 X2 I NOINT P SEQB SSl CLM; OUTPUT OUT=NEW P=YHAT R=RES U95M=UPPER L95M=LOWER; ~ PRrC PLOT DATA=NEW; PLOT VIELD*LEVEL=~T; PLOT RES*VHAT I VREF=O; PLOT VHAT*LEVEL='P' UPPER*LEVEL='U' LJWER*LEVEL='l' I OVERLAY; A plot of the raw data as well as a residual plot and plot of predicted values for the model in part :E) . " PROC UNIVARIATE NORMAL PLOT DATA=NEW; VAR RES; More analysis of residuals from the model in part ]:, . l PRJC ~ ~ GL~ OATA=SCAB; CLASS TMT LEVEL; MODEL YIELD = TMT*LEVEL I NOINT P; FSTI~ATE 1 T~T' T~T*LEVEL 1 1 1 -1 -1 -1 ESTIMATE 1 8 LIN' TMT*LEVEL -4 -1 5 -4 -1 5 ESTIMATE 'T*B LIN' T~T*LEVEL -4 -1 5 4 1 -5 [STIMATE 'B QUAD' TMT*LEVEL 2 -3 1 2 -3 1 ESTIMATE 1 T*B QUAD' TMT*LEVEL 2 -3 l -2 3 -1 OIVISOR=3; DIVISOR=S•; I DIVISOR=42; I DIVISOR=l08; I OIVISOR=54; I I Recall that b2 01 = ~tiy. where the J,. 's may be computed from, say, the ORTHO . ~ 2 ~ -3 1 algorithm. In this example t 1 =54'' t 2 =5Ji: and t 3 =54. I f the levels of the quantitative factor had been equally spaced, then the J,i's could have been obtained from a table of orthogonal polynomial coefficients. -51-· Fitting the cell means model and examining single degree-of-freedom contrasts which correspond to linear, quadratic, treatment and the respective interaction terms. The results are identical to those of part ~. • J~S 2 3 4 ~ 6 7 2 9 10 11 12 1'3 YJHO TMT LEVEL ') F F F F 3 9 16 4 30 7 21 9 16 to 22 18 18 18 24 12 19 10 4 4 5 17 7 23 16 2lt 17 l.r. 15 16 l7 18 19 20 21 :}) 3 3 3 s 3 s c; s 3 3 F 3 6 6 F F 6 6 f s s s 6 6 6 s 6 12 12 12 12 12 12 12 12 F F F F s s s s • The data and indicator variables xo L 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 X01 X02 1 1 1 1 0 0 0 0 1 1 1 1 0 0 0 0 1 1 1 1 0 0 0 0 0 0 0 0 1 1 1 1 0 0 0 0 1 1 1 1 0 0 0 0 1 1 1 1 X1 Xll X12 X2 X21 X22 3 3 3 3 3 3 3 3 3 0 0 0 0 3 3 3 3 0 0 0 0 9 9 9 9 9 9 9 9 9 9 9 9 0 0 0 0 0 0 0 0 9 36 36 36 36 36 36 36 36 144 1'.4 144 144 144 144 144 144 36 36 36 36 3 3 3 0 0 0 0 6 6 6 6 6 6 6 0 0 6 6 6 6 6 12 12 12 12 12 12 12 12 0 0 . 12 12 12 12 0 0 0 0 6 6 6 6 0 0 0 0 12 12 12 12 ~=24 -52- .-l • 0 0 0 0 144 144 144 144 0 0 0 0 q 9 9 0 0 0 0 36 36 36 36 0 0 0 0 144 144 llt4 144 • ~ SEf)lJENTl AL PARAMETER ESTIMATES 13."''33:" 16.4167 16.4167 19.64'58 18.7'5 18.7'5 '>.77083 12.9167 12.9167 X'.l XOl X02 X1 Xll X12 X2 X21 X22 OEP VARIABL~: = y XOjXOl X02\X1\Xll Xl2\X2\X21 X22 SUM OF SQUARES MODEL ERROR U TOTAL 6 18 24 ROOT MSE DEP MEA~l common mean\separate means\common linear\separate linear\common quadratic\separate quadratic - - "'EA~ SQUARE F VALUE PROB>F 4721.000 633.000 5354.000 786.833 35.166667 22.374 0.0001 5.930149 13.333333 44.47612 !;~Q~'!~@ g_:::t I~TERCEPT Standard errors and hypothesis tests are most easily determined from part ~ . TERM IS USED. R-SQUARE IS REDEFINED. NOTE: MODEL IS NOT FULL RANK. LEAST SQUARES SOLUTIONS FOR THE PARA~ETEPS ARE NOT UNIQUE. SOME STATISTICS WILL BE MISLEADI~G. A REPORTED OF OF 0 8R B MEANS THAT THE ESTI~ATf IS BIASED. THE FOLLOWI~G PARAMETERS HAVE BEEN SET TO ~. SINCE THE VARIABLES ARE A LINEAR 'OMBINATION OF OTHF~ VARIABLES AS SHOWN. X02 Xl2 X22 VARIABLE xo X01 X02 Xl Xll Xl2 'X? X21 =+XO =+Xl =+X2 -l*X01 -l*Xll -l:OCX21 ~not of use. PARAMETER ESTIMATE STANDARD ERROR T FOR HO: PARAMETER=O 9. 932877 14.04 7209 q 12.916667 -16.666667 0 1.666667 8 "~.958333 3. 202646 4. 5292?6 ') 0 -0.129630 -0.273148 0.205450 0.290550 OF q B 0 g I' • YIELD ·1F NOTE: NO which is: • -6.16667 = YF- Ys -6.16667 1.4E-14 = 0 -6.16667 1.7E-14 -0.46131 = bl.O (common linear) -4.375 1.7E-14 -.333333 -. 255952 = bFl.O- bsl. o -4.375 1.7E-14 -. 333333 -.255952 1.1E-13 = 0 -4.3 75 1.8E-14 3'.77381 -.255952 1.6E-13 -.266204 = b2.0l (common quadratic) -16.6667 3.3E-14 1.66667 3.95833 -2.2E-13 -0.12963 -.2131 48 = ~2.o1 -bs2.o1 -16.6667 3.3E-14 1.66667 3.95833 -2.2E-13 -0.12963 -.273148 1.2E-l2 = 0 SOURCE c.v. Fitting the model sequence . . > ITI TYPE I SS 1.3()0 -1.186 0.2099 0.2509 0.520 0.874 0.6091 0.3937 -0.940 0.5360 0.3596 4266.667 228.167 0 11.502976 5.502976 0 118.080 31.080357 . . -0.631 PROB . . -53- • • ]: OBS 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 ACTUAL •.• , 9.000 16.000 4.000 ?0.000 7.0(10 21.000 9.001) 16.000 10.000 19.000 18.000 18.000 24.000 12.000 19.000 10.000 4.000 4.000 5.ooo 17.000 7.000 1o.IJOO 17.000 Results of the P and CIM option PREDICT VALUE STO ERR LOWER951 UPPER951 MEAN RESIDUAL PREDICT ltEAN (Cell Means) •.•oi1 9.500 9.500 9.500 16.1501 16.750 16.750 16.750 15.50 15.500 15.500 15.500 B. 18.250 18.250 18.250 5. 750 5.750 5. 750 5.750 14. ,.~ 14.250 14.250 14.250 SUM Of RESIDUALS SUM Of SQUARED RESIDUALS 2.965 2.965 2.965 2.965 2.965 2.965 2.965 2.965 2.965 2.965 2.965 2.965 2.965 2.965 2.965 2.965 2.965 2.965 2.965 2.965 2.965 2.965 2.965 2.965 3.271 3.271 3.211 3.271 10.521 10.521 10.521 10.521 9.271 9.271 9.271 9.271 12.021 12.021 12.021 12.021 -.479346 -.479346 -.479346 -.479346 8.021 8.021 8.021 8.021 15.729 15.729 15.729 15.729 22.979 22.979 22.979 22.979 21.729 21.729 21.729 21.729 24.479 24.479 24.479 24.479 11.979 11.979 11.979 11.979 20.479 20.479 20.479 20.479 -.500000 -.500000 6.500 -5.500 13.250 -9.750 4.250 -7.750 0.500000 -5.500 2.500 2.500 -.250000 5.750 -6.250 0.750000 4.250 -1.750 -1.750 -. 750000 2.750 -7.250 1.750 2.750 2.34479E-13 633 -~- • :e & SEO!~NT!AL Fitting the model sequence which is: PARAMETER ESTIMATES • • XOl X02\Xl\X2 separate :neansicommon linear!common quadratic 10.25 = YF 1:!.25 16.4167 = Ys 13.4 792 19.6458 -0.46131 = b1.o 1.5 7.66667 3.64583 -.266204 = b 2 . 01 XOl xo:: x1 X2 OEP H•IABLE: YIELD SOUl:::: MOO'",_ 4 ERR:c< 20 24 U r,.AL ;IJCT "1SE :JEP "1EAN :.v. NOT~: ~J VARl•BL!' DF xoz 1 1 Xl X2 1 1 oos ACTUAL 1 9.00:> 9.000 16.000 4.000 30.00') 7.000 21.000 9.000 16.000 10.00:> 18.00') 18.000 U!. 000 24.00J 12.0 00 3 4 5 6 1 B 9 to 11 12 13 14 15 MEAN SQUARE 4684.417 669.583 5354.000 5.786118 13.333333 43.39589 I~TERCEPT XOl z SUM OF SQUARES JF F VALUE PROB>F 1171.104 33.479167 34.980 0.0001 ~~Q~!:~ c.:~:: TERM IS USED. R-SQUARE IS REDEFINED. PARANIETER ESTIMATE STANDARD ERROR T FOR· HO: PARAMETER=O PROS > ITI 1.500000 7.666667 3.645833 -0.266204 6.954049 6.954049 2.209610 0.141747 0.216 1.102 1.650 -1.878 0.6314 0.2833 0.1146 0.0750 PREDICT STO ERR LOWER95t MEAN VALUE PREDICT ,=res":ric"';ed cell means) 5.\14 10.042 2.362 5.114 10.042 2.362 5.114 10.042 2.362 2.362 5.114 10.042 11. 281 16.208 2.362 2.362 11.281 16.208 16.208 2.362 11.281 16.208 11.281 2.362 13.792 2.362 8.864 13.792 2.362 8.864 8.864 13.792 2.362 13.792 2.362 8.864 19.958 2.362 15.031 15.031 19.958 2.362 19.958 2.362 15.031 TYPE I 55) 1260.750 3234.083 71.502976 118.080 not useful MEAN RESIDUAL OBS ACTUAL PREDICT VALUE -1.042 -1.042 5.958 -6.042 13.792 -9.208 4.792 -7.208 2.208 -3.792 4.208 4.208 -1.958 4.042 -7.958 16 17 18 19 20 21 22 23 19.000 10.000 4.001) 4.000 5.000 17.000 7.000 16.00:> 17.001 19.958 6.917 6.917 6.917 6.917 13.083 13.083 1?.083 13.083 UPPER95~ 14.969 14.969 14.969 14.969 21.136 21.136 21.136 21.136 18.719 18.719 18.719 18.719 24.886 24.886 24.886 24 SUM OF RFSIDUALS SUM OF SQUARED RESIDUALS -55- STO ERR LOWER951 UPPER951 PREDICT MEAN HEAN RESIDUAL 2. 362 2.362 2.362 2.362 2.?62 2.362 2.362 2.362 2.362 ts. o:n 1.989 1.989 1.989 1.989 8.156 8.156 8.156 8.156 1.34115E-13 669.5833 24.886 -.958333 11.844 3.083 ll.844 -2.917 11.844 -2.917 11.844 -1.917 18.011 3.917 18.011 -6.083 18.011 2.917 18.011 3.917 • ~· GENERAL DEPENOE~T V~~fA~LE: ~OOELS General Means Model • GENERAL LINEAR MODELS PROCEDURE PROCEDURE CLASS LEVEL INFORMATION YIELD JF SOURCE LINEA~ :.,V SUM OF SQUA~ES MEAN SQUARE F VALUe !o!ODEL 6 4 721.00000000 786.83333333 2 2. 37 E~RDR 18 633.00000000 35.16666667 PR > F UNCORRECTED rnTAL 24 5354.00000000 R-SQUARE c.v. STD OEV YIELD MEAN 0.88l77l 44.4761 5.93014896 13.33333333 OF TYPE I SS F VALUE 6 4721.00000000 22.37 0.0001 OF TYPE IV SS VALUE PR 6 4721.00000000 22.37 0.0001 0.0001 CLASS LEVELS VALUES PH 2 F S LEVEL 3 3 6 12 NUMBER OF OBSERVATIONS IN DATA SET = Note that the parameter estimates are the same as those given in part under the SEQE output. Here we also have the standard errors and t-tests; however, we had to calculate the ~. 's for each cohtrast. JD SOURCE T~T*LEVEL SOURCE T~T* LEVEL PARAMETER HIT B LIN T*B LIN B QUAD T*B QUIW ESTIMATE T FOR HO: PARAMETER=O -6.16666667 -0.46130952 -0.25595238 -0.26620370 -0.27314815 -2.55 -1.43 -0.40 -1.83 -0.94 F PR PR > F ~ > F > ITI STO ERROR OF ESTIMATE 0.0202 0.1710 0.6971 0.0835 0.3596 2.42097317 0.32351615 0.647')3230 0.14527499 0.29054999 ( Ce 11 means) LEVEL 08SERVATIC1N l 2 3 4 5 6 1 8 9 10 l.l ~2 OBSERVED VALUE 9.00000000 9.00000000 16.00000000 4.00000000 30.00000000 7.00000000 21.')0000000 9.00000000 16.00000000 10.00000000 18.00000000 18.00000000 PREDICTED VALUE ( = cell means) 9.50000000 { 9.5000.JOOO 9.500000')0 9.50000000 16.75000000 { 16.75000000 16.75000000 16.75000000 15.50000000 { 15.50000000 15.50000000 15.50000000 RESIDUAL -0.50000000 -0.50000000 6.50000000 -5.50000000 13.25000000 -9.75000000 4.25000000 -7.75000000 0.50000000 -5.50000000 2.5')000000 2.50000000 -56- ....-;._:· 24 'IM'r 3 6 12 F 9.50 l5.50 5.75 s 16.75 18.25 14.25 The standard error of each cell mean is .; --·<5.1667 , =2.965. The cell means and standard errors could have been printed by using the option: LSMEANS TMT#LEVEL/STDERR; :e • ® continued GENERAL LINEAR MODELS PROCEDURE DEPENDENT VARIABLE: YIELD OBSERVATION 13 14 15 16 17 18 19 20 21 22 23 24 OBSERVED VALUE PREDICTED VALUE RESIDUAL 18.00000000 24.00000000 12.00000000 19.00000000 10.00000000 4.00000000 4.00000000 5.00000000 17.00000000 7.00000000 16.00000000 17.00000000 {18. 25 000000 18.25000000 18.25000000 18.25000000 r-75000000 5.75000000 5.75000000 5.7'5000000 -0.25000000 5.75000000 -6.25000000 0.75000000 4.25000000 -1.75000000 -1.75000000 -0.75000000 2.75000000 -7.25000000 1.75000000 2.75000000 ~4-25000000 4.25000000 fto25000000 _4.25000000 SUM OF RESIDUALS SUM OF SQUAllED RESIDUALS SIH1 OF SQUARED RES I DUALS - ERROR SS FIRST ORDER AUTOCORRELATION DURBIN-WATSON D Note: ---- o.oooooooo 633.00000000 0.00000000 -0.63467615 3.25701027 In order to get the classical ANOVA table as well as the single degree-of-freedom contrasts and means with standard errors we could have used the statements: PROC GIM; CLASS 'lMT LEVEL; MODEL YIELD= 'lMT LEVEL 'lMT*LEVEL/P CIM; [ same ESTIMATE statements] LSMEANS 'lMT LEVEL 'lMT4tLEVEL/STDERR; Note: To obtain the same results as in @we woulC' fit the morel with interaction restricted to be zero That is, PROC GIM) CLASS TMl' LEVEL: IDDEL YIELD '" TMT LEVEL /P CIM; -57- • :e • ALCOHOL-DRUG uATA WHULC; !~PUT Yl Y2 Y3i S·JdJECT = _N_i ALC.LiHOL = •vES'; Analysis of a IF-~-> b THE~ ALCOHOL='NU'i YS !Yl+YZ+Y31/SI.iKTI31; XG = 1; C.At<.JS i = ~KUC SOkT; 8Y ALCOHOL; Pri.i.JC. P~INT N; ® t>ii.uC GLM; CLASS ALCOHOL; MODEL YS = XO ALCOHOL I NOINT; LS~EANS ALCOHOL I STOERR; ESTIMATE 'DIFFERENCE' ALCUHOL l -l; © DATA SPLif; SET WHOLE; Y=Yl; ORUG• 1 A1 ; OUTPUT; i=Y2; DRUG= 1 8 1 i OUTPUT; Y=Y3i DRUG= 1 C1 i OUTPUT; OKOP Vl-Y3 YS; QD P~u(. PKl~T Ni i.IY PKOC ® Pi-f.(. Y3 = Control response B response A res~onse Yl+Y2+Y3 13 ALCOHOL; PRGC SURTi BY AlCOHOL SUBJECT; ® = Drug = Drug = p. 280. Spli~-Unit ~xperiment Yl Y2 YS h DA~, Rearranging the data to enable analysis of the subplot factor. PROC SORT must be used on the CLASS variables used in the ABSORB statement below. ABSORBing the whole plot factors reduces the storage requirements and hence the time and cost of the analysis. GLM; ~oSORB ~LCOHOL SUBJECT; CLASS JKUG ALCOHOL; I'IOuEL Y = DRUG ALCUHOL*DRUG; Effects model for the subplot factor and main effects contrasts. lSTIMATE 'MAIN EfFECT: AB VS ~· JRUG -1 -1 2 I DIVISOR=2; FSTI"''ATE 'MAli~ EfFEC.T: A VS 13 1 uRU" l -1 J; l;L ·~; A~SURu ALCUHCL SUBJECT; -;G- • • • CLASSES ALCuHOl DRUG; MODEL Y = ALCOHOL*DRUG I NOINT SSl SS2; E~TIMATE 'AB VS CONTRuL @ ~0 1 ALCOHOL*DRUG -l -1 2 0 0 0 I DIVISOK=L E; ~STIMATE 'A VS B ~ NU ALCUHuL 1 ALCOrlOL*DRUG l -1 J 0 0 O; t~TlMATc 1 AB VS CONTKOL ~ VES' ALCOHOL*DRUG 0 0 0 -l -1 2 I DIVISOk=2; ~STI~AIE 'A VS B @ YES ALCOHOL' ALCGHOL*DRUG 0 0 0 1 -1 O; ® General ® ~eans model and simple effects contrasts. PkOC GLM; CLASSES SUBJECT ALCOHOL DRUG; Y = XO ALCOHOL SvBJECTlALCOHOLI ALCOHOl*DRUG I SSl SS2 NOINT; TEST H=ALCUHOL E=SUBJECT(ALCOHOLJ I HTYPE=l ETYPt=l; CuNTRAST 'ALCOHOL OIFFtkENCl' ALCOHOL 1 -1 I E=SUBJECTIALCOHOLJ ETYPE=l; eSTIMATE 1 Ad VS CONTROL ~ NO' URUG -1 -1 l ALCOHOL*DkUG -1 -l 2 0 0 0 I DIVISOR=2 E; ESTIMATE 'A VS B i NO ALCOHUL 1 JRUG 1 -1 0 ALCOHOL*DRUG 1 -1 0 0 0 O; ESTIMATE 1 AB VS COhTRUL ~ YES' JRUG -1 -1 2 ALCOHOL*DRUG 0 0 0 -1 -1 2 I UIVISGR=Z; ESTIMATE 1 A VS 6 ~ YES ALCOHOL' DRUG 1 -1 0 ALCOHOL*DRUG 0 0 0 1 -1 O; GJTP~T OUT=NEW2 P=P R=R; ~u)EL This analysis perforos both the whole-plot and sub-plot analyses all at one time. The simple effects contrasts are more difficult to speci~, and the computing costs are much steeper. DRU~ JD • ~~OC PLGf; PLuf K*P='*' I V~EF=O; Analysis of residuals PROC ANOVA may 'te '.lsed since this experiment is balanced. The PROC 4NOVA; CLASSES SUBJECT ALCOHOl DRUG; EST1MATE option is not available, but the cell means and SS MODEL Y = ALCOHOL SU8JECT(ALCOHOL) DRUG ALCOHOL*DIWG; for the ANOVA table are computed and F-tests made. TEST HzALCOHOL E=S~BJECT(ALCOHOL); MEANS ALCOHOL SUBJECT(ALCOHOL) DRUG ALCOHOL•DRUG I DEPONLY; -S"J-- :. • • ---------------------------------- ALCOrlOL=NO ---------------------------------- oes Y1 Y2 Y3 2.5~ 2.63 2.7"3 3.3d 2.ld 2.12 2.53 ~ 2.d3 2.93 3.58 4 L.':fti 5 2.32 2. 73 ., 0 2.42 .3.99 3. u7 2.15 3.23 :.i.JBJECT YS 7 4.o24!HI 4.o6499 6. 32199 5.09800 3. 8041<t 4.90170 8 9 10 ll 12 xu l l 1 1 1 1 N=6 --------------------------------- ALCOHUL=YES ---------------------------------CJ:3S 7 & 9 10 11 12 Yl Y2 Y3 SUAJECT YS 3. 5o 3. 79 4.J4 J.dS 5.32 4.38 3 .o 3 3.o3 3.26 3.49 3.79 1 2 3 3.03 3.05 4 5 6 6.270J2 6.44323 7.62102 5.93516 5.76773 5. NOU2 4.09 3.1C 3. 33 3.35 z.so xo 1 l l l . l 1 N=6 -60- ® See p. 283 of Allen and Cady for a discussion of the assumed model. ;. • LINEAR MODELS PROCEDURE ~ENERAL CLASS LEVEL lNFJKMATlON LlVELS C. LASS ALCOHOL ~ • .c.nalysis of s•.1ms to test for .,:hole--plot I alcohol~ ::.::.:=-:=-erences. VALUES NO YE:S 2 NUMBtR uF OBSERVATIONS IN DATA SET = 12 DEPENDENT VARIABLE: YS SOURCE ')f SUM QF SQUARES MEAN :)IJUAkE MODEL 2 382.70960556 191.35480278 328.0J ERROl<. LO 0.58339611 PK > F UNCORRE~TEJ TOTAL ~4=ss 12 due to SUBJECT(P~COHOL). 3od.54356667 1- :p) OBS 0.0001 ALCOHOL xo 7 NU 1 NO Nu 1 3 7 7 1 4 8 1 z.·n 9 1 l 2.42 2.13 3.513 3.99 3.38 2.98 3. J I 2.7tl YS MEAN 1.3.6304 0.76380371 5. 603C>o 54'> 5 9 PR > F 1 8 9 NO NLl NO NO NO ·) 9 ~0 1 1) 10 l~u l 11 1C 12 13 10 .11 NO NO r~o 14 11 ~0 15 L1 lo l7 12 NO NG TYPE I SS OF xo 1 1 ALCOtiG.L SOUKCE .)f- xo ALCOHOL F VALUE 376.81280278 5.89680278 645.90 O.OJOl 10.11 0.009ti TYPE IV SS F VALUt: > f O.JOOOOOOO 0 5.89bci0278 E ::> T I o-IA TE T FuR HO: PARAMEHR=J -1.40199890 -3.18 PARAI-IETE~ i>K 10.11 PR O.UO'id STJ t:t\«.uR lJF b T !MATE 0.0098 J.'t<t0'>1ts22!i LEAST SQUARES MEANS ALCOrlCL NO YES 4. YS LSME:AN STO E RK LSMEAN PROB > JT I HJ:LSMEAN=O ~0266604 J. 3ll82l!l6 0.31182156 0.0001 0.0001 b. 30't66494 13 > lT I 1'1 20 a 12 12 1 1 ~-= 24 25 1 2 2 2 3 26 3 21 2d 3 4 29 '+ ::lJ H 4 32 5 21 > ' "-~ u ~ 5 NO NO YES YES YES YES Yi:S YES Y[S YES YES YES YES YES YES YES YES YtS 1 1 ' .L l 1 1 1 1 1 l 1 2.:33 2.55 2.o 3 ~.32 l 2.15 2.12 2.73 3.23 2.53 3.56 4.04 3.26 3.79 3.88 3.4<i 4.09 5.32 3. 79 3.10 4.38 2.80 3.33 3.o3 1 3.J3 1 1 1 1 l 1 . l 1 1 1 l 1 DRUG A B c A B c A 8 c A B c A B c A b c A 8 c A ~ c A B c A B c A B (. A () Yi:S l l 3.35 35 3.63 i::l jt, ::, 'l't.) 1 3.:;::; 34 "' y 1 z 0 01 FF t:RENCE SUBJECl STU i.JEV SOURC.E ®. Com-pare SS found on this -page with those in. say.. analysis vALUE c.v. R-SQUA>..E 0.984'1:!5 ~ t- " ...r • • GENERAL LINEAR MODELS PRuLEOliRE CLASS LEVtL 1NfuKMATION CLAS:> NU~dER VALUES ORJG 3 A B C HCOHGL 2 NO YES OF GBSERVATIUNS IN DATA SET ~ENERAL DEPENDt~~ LEVELS = 36 LINEAR MODELS PROCEDURE VAR!AdLE: Y SUM SQUARES SOURCE Jf MODEL 15 14.47667500 0.96511167 ERROR 20 1.41262222 J.J70o3lL1 CORRE:CT:OJ TwTAL 35 15.88929722 R-SQlJA?:: c.v. STO OEV Y 1'1E:AN o. 911-J·l•, 8.2146 0.26576514 3.2352T77d JF SOURCE SUBJECTI~LCLHCLJ DKUG DRUG*AL C <jr1i.JL SOUHLt. DRUG DRUG>I<L\L ', CrlOL UF fYt>E I SS 2 2 5.s9oaoz7o 5.ti33961ll 1.87722222 0.868688!l9 OF TYPE i.V SS 2 2 l.d17 Z2222 o.S6do8il89 1 ALCOHOL 10 MEA1~ l[ t< 11Alt'i t:.HEC.T: !\o V:) C HA IN E: Ff £ C T; A VS B f VALUE: f VALUt l3.oo PR > f PR > F ~ F 13.2':1 J.JC02 o-15 J.JJSJ VALUE ,>K 13.29 6.15 o.oao2 ESTIMATE PARA liE TER=O -).40416667 -4.30 -2.64 -o. 30833333 5-.IUAI\E 0.0001 T hJK HO: PARA:~E • > F Note that ALCOHOL and SUEJ~CTfAtCOHOL) are not included in the Type IV SS. J.OO.H P~<. >Ill STu EI<KOii 01' t:~ f l MATE J.JJJ~ 0.0'1Bo2ll 0.0101 0.108~9817 -62- These contrasts ar~ Qf l~ttle interest since the DRUG*ALCOHOL interaction is clearly important. ,. ~ OEPENDE~l GENERAL LINEAR MOUELS • PROCE~URE General :r.ea11s model with whcle-plot factor ABSO?.:::ed. V4RIABLt: Y SOURCE (JF SUI-I OF SQUARES MODEL 15 14.47667500 0.96511167 13.66 ERROR. 20 1.41.262222 0.07063111 PK > F CORRECTEC TJTAL 35 15.88929722 R-S~uAki: c.v. sro oEv 0.91.1096 d.2l46 o.2o5765l't SOURCE: JF- ALCO rlDL. SUBJECT(ALCC.HGL) AlCOHOL*DI<uu l 10 SOURCE DF AlCOHOl*JRuG PARAMETER AB VS CUNTKUL ~ ~~ ~ NO ALCOHOL AB VS ~uNTkUL ~ YeS A VS d ~ YES ALCOHOL A VS B • 4 '+ :'iEAN Y VALul MEAt~ 3.23527778 F VALJE 5 .!!96602 76 5.83396111 2. 74591111 83.49 13.26 Pk. > F 0. J JO l ~.72 0.0001 0.0002 II SS F VALUI: PK > F 2. 745'llll.l 9.72 0.0002 TYPE: F 0.0001. 1 SS TYPE S~uArd::: T FOR HO: PR > ITJ STU ERROR OF EST I MATE PARAMHER=O ESTIMATE ·-------- ----------·- ------------------ -().20333333 -0.00666667 -0.60500000 -0.61000000 --------- -I..SJ -0.04 -4.5::> -3.98 0.1416 0.9o5o J.JI)OL 0.0007 -6:3- J. U2od25/ o.l53<+395b J.l3288257 o. l531t-39!18 This is a set of simple ef:'ects contrasts (see Table 22.3, p. 2Cl - ~llen and Cady). • Dt:t-'ENU.CNT ·~ Comcined whole-plot and split-plot analysis with \itNERAL Ll~EAR MODELS PROCEDURE vA·< 1 •<tL:O: si~pl~ects ~ SOU'<CE :JF Su."\ OF SI./UAr\ES MOuEL lo 3'il.28'J47U8 24.4555'/236 ERROR 20 lo4l2o22l2 J.J7Qo3lll 3b 392.70210000 R-SQUM~E c.v. STU JE:V Y Mt:Af'< 0.946-'>03 il.21H 0.2657o514 3.2352711b TOTlL UNCOR~ECT~J SOUKCi: OF xu TYPE I ss ri,£.\N Si.OJAF<.t F VALUe > Pk ~0.0001 ALCOHCL~Ot'.JG SOURCE JF TYPE II SS F VALUE: PR xo 0 1 10 2 2 0.00000000. 5.89680278 5. 83396111 1.87722222 o.8o8o8d89 DRUG ALCOHOL*OR.J.> Of HYPOfHE:~~S TYPt l SS JF ALCOHOL USINC THE TYPE 1 CONTRAST ALCuHGL JIFfE~ENCE PARAMETER AS (..'Ji~T.J ,,5 A 1/) AB VS A Vi .. 13 a• "l'J t CuNr~UL ~ ~ Y£~ Ql Nu .\ICJdJl. JJ YES ~LCOHGL ~S ~:; 1 ~.89680278 f- o.ovo2 0.0083 > F ~ O.OJJl 0.0002 0.JOd3 F VALUE PK ) 10.11. f f A~ AN VALLlt PR > lO.ll 0 .o 09(i T FOK HO: Pk. TE~M Compare with the analysis in part 0• ""' J.009o FGA 5USJECTIALCJHOLl JF > . 8J.49 d.2o u. 29 6.15 '>.89680278 HYP~lHE~ES Pr\ USING THE TYPE l MS FOR SUBJECT(ALCOHULI AS AN tRr\WK SOU!< C.t TESTS GF .; ... b.L't ~ 5334.94 83.4'7 8.26 13.2'1 o.15 ALCOHOL SUBJECT(ALCUHULl VALJc f 376.d1280278 5.896d0278 5.83346111 l • b 77 22 2 2l. O.d6868d89 l f J.OOOl l 10 2 2 ALCDHCL SUBJEC J( ALCC..Hl.'ll ORU& TE~T~ • ·:cntrs.sts. > ITJ E~KuK TE~M t STD EKii.l..lK Uf E:STIMATE PARAME TER=O -0.2033333.3 -1.~3 J.l~l6 -O.JOo66:.C:. 7 vol.32otl2~/ -0.04 o.~o5d -0.60500000 -0.61000000 J.l:>!>'t395b -4.55 J.0002 -3.98 O.JJ07 0. U2ocl257 J. 1 '>343'158 tSTIMATE Gimple effects as :in ®. • k 0.4 SYMBOL uSED IS PLOT OF R*P I I • * * + @ NO YES 0.2 + + * * * * * * y 7 8 NO NO NO NO NO NO YES YES 3 2.67000000 2.69333333 3.65000000 2.94333333 2.19666667 2.8300000() 3.62000000 3.72Jil00J;) 4.40:JOOOOO 3.42666657 3.33000000 3.34333333 6 -o.z A ~ + * I I I I * * * c * * * * * * -0.3 + 3 3 3 3 N y 12 12 12 3.21583333 3.52416667 2. 96583333 ALCOHOL DRUG N '( NO NO NO YES YES A 6 6 z. !l950000() 2.90166667 2. 6950000() 3.53666667 4.14666667 3.23666667 res * >l< 3 3 YES YES l'ES * * 3 3 3 3 3 res 3 4 5 * -0.1 + * 2.83055556 3.64000000 N DRUG I I I 18 18 ALCOH:>L 10 11 12 1 2 0. 0 +- -·- --------------:- --:--------------------------------------------------- l y SUBJECT 9 * * * * * * N ALCOHOL * * ~ROCEOURE HEANS * * • OF VARIANCE * Residual plot O.J + o. l b A~ALYSIS B c A s c 6 6 6 6 -O.'t + --+---------+---------+---------·---------·---------+---------+---------·2.0 3.o 3.5 4.u s.s z.~ ~.s ~.J p -65-- Note: Only the ME.~~2 output is included from the PROC ANOVA output. Although all the appropriate SS have t-een obtained via PROC GLM, PROC ANOVA Kould be less costly. ~· ~RUC (Brogan & Kutner, :cs-:::c. The Americ_an Statistician ~:+:22')-232) These statements calculate the sums and differences re~uired for the appropriate t-tests. See printout in ~art '];) and JV. SORT; BY GRUUP; ~ PkwC PRINT; BY GKOUP; !, PROC TTEST; CLASS ~ROUP; VAR TMT PREPCST TMT_X_PP; ~ PKuC GLM; CLASS GROUP; MJOEL TMT PREPJST TMT_X_PP LSMEA~S ~ROUP I STOERR; ESTIMATE 'NE~ VS STU' GROUP @ • UREA SYNTHESIS IJATA - Ana::.::sis of a repeated measures experiment DoHA SHUNT; INPUT P~E PUST ~~; SULiJt:C T = -~~-; IF _N_ LE 8 TnEN GROUP='NEri 1 i ELSE GROUP='Ol0'; XO=l; PREPGST = PUST - PKE; TMT = PGST + PRE; TMT_X_PP= PKEPGST; IF GROUP= 1 0LD 1 THEN PREPUST=-PREPUSI; lAr<JS; This gives the three appropriate t-tests for treatment effects, time effects and interaction. XU GROUP I NOINT; -1 i DATA REPEAT; SEI SHUNT; Y= PRE; TIME=l; OUTPUT; Y=PUST; TIMt=2; UUTPUT; DRJP PKt POST TMT PkEPOST TMT_X_PP; Same analysis as in @ except the analyses are performed using 1-way Al!!OVA. Compare with tEl. Note that more than one dependent variable may appear i;o the left of the equal sign in the MODEL statement. Rearrange data for a "split-plot" tYIJe analysis. PkuC PRINT; VAR SUBJt:CT XO GROUP TIME Y; 6 PRUC GLM; CLASS GRuUP TIME SUBJECT; MODEL Y = XC GROUP SUBJECTIGRJUP) TIME TIME*G~OUP I ~~INT SSl SS2 SS3 ~54 P; M~ANS G~UUP SUBJECTIGK0JP) TIM[ TIME*G~OJP I JEPON~Y; lEST H=~kUUP E=SUBJECTIGKOUPI I HTYPc:l cTYPt:l; OUTPUT OUT=PluT RESIOUAL=KcS ~k~uiCIEu=P; J: P~UC PLOT; PLOT RES*P='*' I VREF=O; Residual plot. -66- Combined ANOVA :able with two error terms. • ~ ---------------------------------JbS • • • i>f< E GRuJP=~~n r'UST :.i.JdJECT 4il 1 1 XCJ ----------------------------------Pi'-EPU:)T r-.. r fMT_X_Pt' '>19 -3 20 Tl:e assumed model yijk = 1 2 51 35 :J'j c: 1 3 4 61) 60 'TO 39 46 52 36 43 46 3 4 5 6 1 1 1 1 7 i 't2 54 8 1 5 6 7 6 3~ -3 20 '>J -5 -3 -3 -6 12 i -5 -3 uib: 12 ~ ---------------------------------- ~ROUP:OLu ----------------------------------JbS 9 lJ 11 PkE i>GST 34 16 3o lo ld 32 'tJ 34 36 15 .;a 14 15 16 17 j2 44 50 u:J ld 63 2J 43 45 0 7 50 36 11 12 13 14 15 16 17 1d 19 't2 :.3 34 20 32 21 21 14 PR& 1 1 1 't 10 12 19 20 xu SudJECT 1 1 1 l l 1 1 1 1 1 f'kEPUS T 18 :)J -18 4 7o -4 1d 1B :)0 -1o 54 b 70 18 24 4o 64 7 '13 15 -4 14 105 -18 -6 -18 -24 -7 -15 130 4 do -14 8 l1 7o -a 75 -11 1, 2; 1,.- ·, n 1 ; ith treatment effect 'rk kth time effect (cc:·\k c.:'(i) €ij'l1 treatment by time interaction effect of subject j nested within ith treatment group random error Eij'H- N(O, 0'~) 0.J (.~ ) - -2' N( 0 -~ ~ ')' Yijk ~ N(u,~,~;+~2) --- POST = postoperative response Pre Post New (n:8) ~11 ~12 Old 'n=l:O) ~21 ~22 Treatmf'~t :Iote the.t: il>i'I'_):_?P PP~POST = = { . - y ij1 = d~J .. ~ij2 dij -dij if if (=within subject differences) group =NEW group= OLD P?'F?-'ST ~ :_: ; ;_eer1 Er 'Fe -·)Wr: 'r'PEST a•.-:.c~!la"" -'-:'!'":,_~ 'IMT = Yij1 + Yij2 z1 ~ ( = within sub,ject :o·..t.'!l~' 1.2 overall mean preoperative response c k cell mean of ith treatment, kth time combination .yi TMT_X_PP T/oiT -'- :; :: i) + Eijk where: -3 -6 'Hl '16 be written as: ~ik = u-'-~'i +-:k+(a-r)ik -6 12o 75 75 d9 -6 11 ik ~ay s ·~n: 1:· ~-=:}~ec r\- r-=-.2\ -; •' :"f'~rer1r.:e.2 2 • E • \IAI{l ABLE: -b t-test uses TMT zl- z2 "difference of sums" r.. ;~EAI~ STu OE V NE:Io 0 13 ':13.:>i:JOL000 75.CJCOC.JOJ lo.1o87~2'13 OLO 2't.LJd3<j929 GROuP VARIANCES I DF UNEo~UAL 2.0'~04 EQUAL l.'1J44 18. 8 19.0 • TT EST PtWC EUURL 50 "1Ii'li1'1UM 1-IAXlMJM :).71oSl742 7~.0JJOJOJJ 6. 122.5U 42. 'tt..JJJJJJJO 12o.uJuJJOu UJ.uOJJJJJ STu tRKuR y . ~ r :.o ~ ~ u l l o - - fl12 "'21)( . """ > IT I PROB FOR HJ: VARIA~LES A~t VAKIA8LE: PKEPOST GROUt> t-test uses N NE<I OLD 8 13 EJUAL, F'= ~ITH 12 ANO 1 PKub J~ pre > F'= o.Zo'Jl "sum of differences" "'EAN STtJ OEV STu ci{RUK MI NU4UI'I MAXIMUM 0.75JOOOOO 12. J76923Jd 9.13579551 7.63174db0 3 ... 4212351 2.l1oo6628 -t>.JJOOJOOJ -4.0JOOOJOO 2.0.000JOOOO 24.00000000 Df PROB > IT I -2. dJJl -2.9767 12.3 J .Jl57 l~.O 0.007o \' J ~ UNEQUAL EQUAL FOR HO: VA.<.lfHL[S AKE EJUAL, F 1 -= 1.63 wiTH 7 ANO 12. Uf PR.uB > f'-= 0.4375 -----------------------------------------------------------------------------VARIABLE: TMT X PP GROuP d 0.75CJOOJ0 11 -L2.J76923u:3 VARIANCES UNEIJUAL E.JUAL fOR HO: MEAN ,''4 NEw OLu t-test uses T Jf 3.1743 3.3{01 12. 3 H.O V4KIA'll.. cS AKI: ::.QUJ\L, ~ -~ STLl OE "difference of differences" v 9.1357'>551 /.6.31 74ooJ PROB STtJ tKROk :'-l HI U.'1 MAXiMlM 3.4<t212J51 -o.OOJJJJJO 2.11666628 -24.JJJOJOJJ ZO.OOJOOOJO ~H ~.00000000 > IT I J.007& 0.0032 F'= post These are not helpful due to the significant interaction. (\ + d.2 T VARIANCES II 2.25 ·~ . '/..~22 30 .. J.0498 0.072.l • Since the interaction is clearly important we need to consider simple effects l.6j WITH 7 ANu 12 Jf PkOB > F'= -68- J.~375 • • • GENERAL LINEAR MODELS PROCEDURE _£ CLASS LEVEL CLASS vt<.uuP An alt~~ative ~roach to finding the same information as::.~ :;:arts (!;) or@. INFO~~ATION LtVtLS VALUES NErl OLJ 2 hiJMdi:R Of OdSEkVATIUNS 1N SET ~ATA = l1 DEPENDENT VARIABlE: H4T SOUkCE 01- SUM OF S..,jUARES MEAN Si.IUARI:: JOO 00000 71 ,31. 5 0000000 467.36::1421()5 MODEL 2 ERROR 19 68d ..). 00000000 21 bl943.ovoooooo R-SCJUAkE c.v. STiJ DI::V HH MEAN 0.941557 26.3490 n.o187J5H d2.047o1905 JF TYt'E: I SS l l l4l3oti.047ol905 lo.;.4.9523d095 UNCOkRECTEJ SOURCE xo GROUP T~TAl Jf SoURCE xo () GROUP l l<t 3063. TYI'f IV SS 0.00000000 lo94.'15l3H095 T fLR HO: PARA~IE NElli \IS TE R STJ ESTIMATE PAKAMETE R=0 lo.,JJJCJOO l. iO f VALuE 153.05 PK ) f O.uOOl f >JALIJI: PK 3 02.4 8 VALUE t>R 3.o3 PK F o.uOOl 0.0721 3.o3 f > > F J.07ll > llj .)f.; I:RRUK Gf E;,fiMAit 0.0721 9. -69- 7145~'Hb • • © • GENERAL LINEAR MOL>ELS PROCEOUitE DEPENDENT VARIABLE: PREPUST OF SUM OF SI.IUARES MEAN SUUARE F VALUE MODEL 2 1900.5 76923011 950.2111146154 13.2!; ERROR 19 1362.42307692 11.101>4111 J PR > F UNCORRECTED TUTAL 21 3263.00000000 R-SQUARE c.v. STD DEV PREPOST MEAN o.5B24113 109.0965 8.46796775 7.761904711 SOURCE SOURCE XO GROUP SOURCE xo GROUP PARAMETER NEW VS STD O. OOO.i! > L>f TYPE I SS VALUE PR 1 1 1265.19047619 635.311644689 17.64 8.86· 0.0005 0.0078 OF TYPE 1V SS F VALUE PR > F 0 1 o.oooooooo 635.3 B644689 ESTlMAIE T FOR HO: PARAMETER=O -11.32692308 -2.98 f . II.B6 GROUP f I . GROUP 0.0078 > Ill STD ERROR OF ESTIMATE 0.00711 3.80515341 PR NEW OLD GROUP DEPENDENT VARIABLE: 1111 X PP Df SUM Uf SQUARES MEAN SI:IUARE F VALUE MODEL 2 1900.5 7692308 950.28846154 U.25 ERROR 19 1362.42307692 11.70647173 PR > F UNCORRECTED TOTAL 21 3263.00000000 R-SI.IUARE c.v. STU DEV TMT_X_I'P t4EAN o. 5112463 117.7664 8.46796715 -7.19047619 Uf TYPE I SS F VALUE PR > F 1 1 1085.71>190476 B14.1l1501832 15.14 11.36 0.0010 0.0032 Df TYI'E IV SS F VALUE 0 1 o.oooooooo 814.111501832 11.31> SOURCE SOURCE XO GROUP SOURI:E xo GROUP PARAMETER NEW YS STU NEW DLO NEW OLD 0.0002 PR > f 0.0032 T FOR HO: PARAMETER=O PK > Ill ES HMATE STO ERROR UF ESTIMATe 12.826'12308 3.'31 0.0032 3.Bv515343 I -70- LEAST SQ~AR~~ HE4~~ JMT LSMEAN STO ERR LSMEAN PROB > III HO:LSMEAN=O 93.5000000 75.0000000 7.6433666 5.9959501 0.0001 0.0001 PREPOST LSMEAN STD ERR LSME:AN PROB > Ill HO:LSMEAN=O o. 7500000 12.0769231 2.9938787 2.3485917 0.8049 0.0001 TMT_X_PP LSMEAN STU EH.R LSMEAN PRUil > I Tl HO: LSME:AN=O o. 7500000 -12.07119231 2.9938787 2.3485917 0.8049 0.0001 • SuBJECT xo GI\OUP 1 2 1 1 3 2 2 l 1 1 1 1 l 1 1 1 l l 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 NEw NEW NEw NEW NEW NEw GB.:i :R) ,'* 3 6 7 3 4 4 5 9 9 10 11 5 6 6 12 1 13 14 15 16 11 1::3 19 2J .21 22 23 24 25 26 27 7 8 0 'I 9 10 10 11 ll 12 12 13 13 14 14 15 15 16 16 17 17 18 18 19 19 20 20 21 21 28 29 3::J 31 32 33 34 35 36 37 3d 3'1 40 41 42 1 2 1 2 1 2 1 2 1 2 1 NEW NEw NEw NEW OLD ULD OLD OLD 1 1 1 1 1 1 1 l 1 1 1 36 34 16 36 18 38 32 32 14 44 20 50 43 60 45 1 2 1 2 OLD OLD OLD ULO LlLO OLU OLD OLO OLD OlD OlD OLD UL!J OLO OLD OLD OLD OLD OLD llLtJ OLD l <tO 2 (JltJ 1 1 ]5 55 o6 60 40 35 3'1 36 46 43 52 46 42 !14 34 16 2 NEW l 51 4b 1 2 l NEW NEw NEw NEW • y l 2 1\I.EW CLASS LEVEL LEVeLS l 2 1 2 l 2 1 2. 1 2. 1 2 1 2. l 2 63 67 50 36 42. 34 1 43 2 32 2 ~E.w TIMt 2 l 2 Ll l~FuRMATIO~ VALUES GkOUP SU!:lJlCT 1 2 • GENERAL LINEAk HOOELS PROCEDURE ® CLASS TIME lJLO 1 2. 3 4 5 6 7 8 'I lJ ll 12. lj 14 1~ 16 17 J.blJ202l -71- \'..) M :-} f ;', (l F Ut_j -~ -:-_ 1•· 1·/ ~ T I 1\j' . ~- ; ') .) ~ +~ -..' • "" .2/ DEPENDENT VA~!ABLE: uENERAL LINEAR MODELS • ~ROCtDURE Y S~o~UARE t- \1 AlUc 76'i21.78tl4o154 33H.4255d528 93.2o 19 681.21153840 37.d5J23d87 42 17603.00000000 R-SIJUARE (:.>/. STu iJE\1 0.991222 l4.~95tl 5.98775142 JF T't'PE l SS 1 1 70oU4.023ti0952 847.47619048 4440.00000000 542.8809523ti 407.40750916 SOURCt: JF SUM OF SoiUARLS . HODEL 23 ERROR UNCOkRECTED TuTAL SOURCE xo GROUP SU6JEC:T L Gk.l.JUP I TIME GKO\JP*T l Mt: 19 1 1 MEAN Pi<. > • F J.OJ01 Y MEAN 41.02380952 I' VALUE 1971.48 23.64 o.sz 15.14 11.36 ?R > f t ~ 1 0 0.0010 0.0032 = (-A)< -0."5) + (M)(l2.0769) {2(35.8532h'[(281)2 (~)+(~i)2 (l~)]}i = 3.89 SOURCE OF TVPE 11 SS xo 0 1 19 O.JOOOOOOO 847.47619048 4440.00000000 54.2.88095238 ;.PtLoOOU,:.>L.:>O 407.40750916 GROUP SUBJECT I GKi.JUP I Tl ME GROUP*TIME SOURCE xo . L i JF TYPE I l l SS 0 1 &ROUP SU6JECTIGKUUPI TIME GROUP*TIME 19 1 1 0.00000000 847.47()19048 4440.00000000 317.69322344 407.407 50916 SOURCE Uf TYPE IV SS xo GROU!l SUBJ EC Tl GfWuP I T I ltf: GROUP* TIME 0 1 19 l 1 GROUP F f VALUE •. L----/ 23.o4 6.52 15.14 A.:.>eL't ll.3o ~ VALUE PR > f 23.64 o.52 S.l:l6 ll. 36 0.0001 0.0001 0.0078 0.0032 VALUE PR > F 23.o4 o.52 0.0001 0.0001 ~ 0.001 o.u032 t:lo86 u.oo1a 11.36 ().0032 SU~JECT(GROUP) l)f TYPE I SS 1 847.47619048 f VALUE 3.o3 F = (3.89) 2 = 15.14 , PR > F o.oooooooo 847.47619048 4440.00000000 317.69322344 407.407 50916 TfSlS Of HYPOTHESES USING THE TYPE l MS FOR SOuRCE f ~ l ~ AS AN tRROR TERM PR > f o.o 721 The T,ype I and II SS for TIME test equality of the weighted means -- weighted according to sample size. This is probably not desired and the Type III or IV should be used as they test equality of unweighted !!leanS, j -"2- C=pare with the results of ® and @. • • GENERAL LINEAR MODELS PROCEDURE ~ • 11EANS t.ROUP ;H:w ULD N '( lo 2b 46.7500000 37.5000000 SUdJ ll.. T GKUUP N '( l NEoi NEW NEw NEW NEw NEW NEw NEW OLD OLD l.JLD OlD OLD uLD OLU OLD OLD OLD OLD ULD OLD 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 49.5vOOOJO 45.0000000 o3.0Jvooou 37.5000000 37.5000000 44.5000000 49.0000000 43.0000000 25.0000000 33.0000000 25.0000000 27.0000000 35.0000COO 23.0000000 32.0000000 46.5000000 52.5000000 65.0000000 43.0000000 33.0000000 37.5Jil0000 2 3 4 5 6 7 8 "10 11 12 13 14 15 16 17 1.8 1';1 20 21 2 2 2 y N TIME 1 21 2 21 44.619047o 37.42d57l4 GROuP Tl Mt N '( NEw NEW 1 2 8 ~LiJ 1 2 13 13 46.3750000 47.1250000 43.533't615 3l.4615j85 CLD a l ~ These means are plotted in part @. j -73- :~' ·" ·~· ·.· • + I I I I I * • I I II I + * I I I I I + I I I I I * * '.I) * * * * * •JJ * 1.1) ::::) _, 0 ·"'>-::!: * * * ** * * * * * * * UJ "X u. 0 .... _, (J 0.. • .j.l 0 rl * PI rl * +0 * I I + I I I I * "' +"' I"' 1 I 1 1 * 1"' 1 1 * +----·----·----·----i----·----+-~=~+----+ 0 0 ~ .... ·rl . l.t'l ~-- l.t'l 0 "' 0 • . . l.t'l 0 "' l.t'l ..... I I • I z. .i.J 0 '::l T V'l .,... +O * . "'"' I I I 1 * 0 ~ I'<"~ p:; IX a.. +O Q) 1.1) ~ * •rl :.u "' I I I I I * ro ,g + 1 * ./'\ "' "' I * .., 0 I I I I I * a.. 1.1) "' + 0 * * <.II .... I ..0 I I * * It'\ + 0 + ':) ,... • ..,., I I •"" I .... l.t'l 0 " • H3MOGLOBIN • - Analysis of a 2-Period Cr8ss-over Design (Grizzle, 1965, J AT A HE!o\U; l:'lt>JT Yl Y2 ,..;,; SJ:3JEC T = -~-; IF _N_ LE 6 THEN GKOUP= 1 Al_B2'; ELSE GKOUP= 1 Bl_A2 1 i f'H = YZ-Yl; i\!:::510= YZ+Yl; Ttl.b-IJ= HIT; iF GKOUP='Bl_AZ' THEN TREND=-TMT; XJ = li L.A.<.JS; • ~ j3j._ometric~ 21: 46;-I+EC) These statements calculate the sums and differences required for the appropriate t-tests. See printout for };) and ]) . PRuC jUKTi BY GKUUP; ~ PKUC ~· PKOC TTEST; CLASS bROUP; VAK TMT RESID TREND Yl; "C' PRUC GLM; CLASS GRJUP; M00£L TMT RESID TREND Yl = XO GkuUP I NUINT; LS~EANS GROUP I STDERRl ESTIMATE •CONTRAST' GROUP 1 -1; ]) O~TA '-./ ~ROC Pf<INT N; BY GROUP; PROC TTEST gives the appropriate four t-tests for treatment effects, carry-over effects, time trend and treatment differences within first period only. AUV; StT HEMO; Y-=Vl; t'E:RIOD=l; If _N_ LE a THE:"i TRT= 1 A1 Y=YZ; PERIOO=Z; If _N_ LE: 6 THEN TRT= 1 B1 ORUP Yl Y2 TMT RESIO TREND; PRl~T; ; ; ELSE: TRT='B'; l.JlJTPUTo ELSE TKT= 1 A1 i OUTPUT; ?KuC GLM; CLASS TRT PERIOO SUBJECT ~ROUP; MODEL Y = XC GROUP SUBJECTIGROUP) PERIOD TRT I NOINT P SSl SS£ SSJ SS~ MEANS GROUP SUBJECT(GROUP) PERIOO TRT I OEPONLY TE5T H=GROlJP E=SUBJECTlGROUP) I HTYPE=l ETYPc=l ESTIMATE 'TRT' TRT l -1; eSTIMATE 'PERIOD' PERIOD 1 -1; :W' PROC PLOT; PLOT RES*P~••• I VREF=O; Combined ANOVA table with two error terms. Residual plot. -'/'~- ®. Rearranging the data so that the "usual" ANOVA table may be constructed. VAR SUBJECT XO GROUP PERIGO TRT Y; ® '-"'--' This performs the same analyses as those found in par:t Compare also with the results in part @ . • QD ---- ___ -------------------------LlBS l l 3 Yl Y2 0.2 1.0 -0.7 0.2 1.1 0.4 1.2 o.o -0.8 O.b 0.3 1.5 't 5 b GKOUP=Al_B SllBJEC T 1 2 3 4 5 6 2 -- • • -------------------------------- TMT RESliJ 0.8 -0.7 1.0 0.5 (). 1 -0.3 1.2 -0.1 -:>.o 1. 7 0.7 2.7 xu IRENU .:>.a 1 1 1 1 1 1 -0.7 1. 0 0.5 o. 1 -0.3 The assumed model appears as: y ijk = uik + ~ij + Eijk where uik N=o j=l,···,n1 ; --------------------------------- GROUP=B1_A2 Y1 uSS 1 1.3 ii -2.3 0.9 1.0 0.6 -0.3 -1.0 1.7 -0.3 o.o -o.a ~ 10 -u.4 -2.9 -1.9 -2.9 11 12 lJ 1-+ SUBJECT Y2 7 8 9 10 11 0.'7 TMT -0.4 3.3 0.6 0.5 -0.6 12 4.o 13 14 3.8 loo = J.L + !Tk + ¢.e + A,e i=l,2; k=l,2; L=l,2 and IU:SID 2.2 -1.3 0.6 -1.1 -1.4 -1.2 -2.2 -z.o xo TREND 0.4 -3.3 -0.6 -0.5 0.6 -4.6 -1 • .:. -3.d = general mean, = the effect of the i-th patient (subject) within the Kh eequence, which, for the eake of testing hypotheses, we must assume to be a normally distributed random variable with mean 0 and variance ..: , .... = the eft'ect of the k-th period, •• = the direet e1fect of the l-th drug, >., = the residual e1fect of the l-th drug, and ••~> = the random fluctuation which is normally distributed with mean 0 and variance or! , and is independent of the f 11 • ,. ~.~ 1 1 1 1 1 1 1 1 The assumptions made· about E11 and e.,. imply that the variance of an obeervation is or! or! and that two observations on an indi"ridual have covariance or! • Observations made on diHerent subjects are dependent. N=8 + Yl are the responses during period l. m- Y2 are the responses during period 2 Note: Group (Sequence) l(n=6) 2(n=8) 1 A J.Ln B J.L21 2 B J.Ll2 A J.L22 Period ( i) (ii) (iii) CY1·1 - Y1. 2) - ( y2 ·1 - y 2· ,) = 2 (tf>1 - (Y1•1- y1•2) + (y2•1- y2·2) = 2 (TT1- TT2)- (:\.1 + A2) + (E1•1- E1·2 + E2·1- E2·2) (yl·l +yl·2)- (Y2·1 +y2·2) = (A-2- :\.1) + 2 (~1· - ~2·) + (E\.1 + €1·2- €2·1- €2·2) (iv) 'lMT = Y1 j 2 -yijl = dij TRE!'m ={ y1·1-y2·1 (=within subject differences) RESID = yij 2 +yijl = zij (=within subject sums) ~2) + ( A2 - A1) + ( €1·1 - €1· 2 - €2 ·1 + €2. 2' rtf>1 -<1>2 ) + (~1.- ~2·) + (€1·1- €2·1' Now, i) through iv) may be reccenized as: d .. if sequence is AB l.J (i) -dij if sequence is BA (ii) (iii) (iv) ~-­ -~c- #;_>:.·. d1 -a2 a1+d2 , a test of treat=ent effects if there is no carryover effect , a test of perio~ effects if there is no carryover effect zl-z2 ' tests for :arr:.-c·ter ef!'e-.: ~" tests for treatment effe:ts based upon the first period data only ]) VA.< I ABL. . . T t-:~st TTEST PROCELlURE uses d.1 - d.2 "difference of differences'' • GROUP '; MEA 'II STU OEV STD ERROR MINIMUI-1 MAXIMUM A1_B2 B1_A2 :, :, O.B33BB 1.b7,00000 O.t-5625198 1.99051321 0.26791375 0.70375270 -0.70000000 -0.6000000C 1.00000JOO 4.ocooouoo VARIANCE~ UNEQUAL EQUAL FOR HO: T -1.9145 tl.~ o.uadz -1.o~l5 12.0 o.t1o5 vA~IANCES VARIABlE: > I Tl ?ROB i,)f A~E EJUAL 1 f'= t-test uses ~ES!J 9.20 WITH 1 AND 5 DF -zl- -z2 ·~ MEAN STO OEV A1_B2 8l_A2 b O.d3333333 -O.dJCOOOOO 1.32614130 1.47't54593 T [jf 2.1732 2..1379 11.5 12.0 VARIANCES UNEQUAL EQUAL FOR HO: VAKl~NCES VARIABLE: Tr'.f:f\10 PROB > f 1 = J.02o5 "difference of sums" GROUP d ERROR MINIMUM MAXIMUM 0.54139737 0.52133071 -0.7000000J -2.20JOOOOO 2.10000000 2.20000000 ST~ > ITI PROS 0.0515 u.05J8 There appear to be important carry-over effects. Thus, the above test for treatment effects is not free of residual effects and we must resort to the results of the first p~r:o1 ~nly (variaole Yl below). AKE EQuAL, f'= 1.24 WITH 1 AND 5 Of t-test uses al + a.2 "sum :>f differences" PROB > F'= 0.8437 GROUP !'-< :olEAN STO DEV STD ERROR MINIMUM MAXIMUM Al_B2 8l_A2 0 o. 23333333 -l.6750JOOO 0.65625198 1.99051321 0.2b791375 0.70375270 - o. 7000000 0 -4.6\lilOOOOO 1.00000000 O.bOJJOOOO PRU8 > F•= 0.0265 3 T Df 2..5342 2..2390 8.9 12.0 VARIANCES UNEQUAL EI.IUAL PROB t-test uses GROUP N Al_B2 6 3 6l_A2 VARIANCES UNhhJAL EQUAL > IT I 0.0323 0.0449 FOR HO: VARIANCES ARE EQUAL 1 F 1 = VARIABLE': Y1 9.20 WITH 7 AND 5 OF yl·l -y2·1 "independent two-sample t-test for the first period only" MEAN STL) OEV STO ERkOR M1NIMUH MAXIMUM ::lo3JJOOOJO o. 75365775 1.50990175 0.30767949 Oo533d3301 -0.80000000 -2.90000000 1.50000000 l.JCOuOOOO -1.237~0000 T 2.49~3 2. 2746 FUR HO: VARIANCES ARE Of PROB 10.8 12.0 > ITI 0.0302 0.0421 E~UAL, _,.-; • . f'= There appear to be treatment effects present. Note: the con~lusion wou11 likely have been different. 4.01 WITH 1 AND 5 DF ~ROB> Had we chosen to :gn~re residual effects F•: 0.1453 :·: ;·~:·:- :. .0 • • GENERAL LINEAR HODELS PROLEOURE DEPENDtNT VARIABLE: TMT j)f SUM OF SQUARES MEAN SQUARE F VALUE MODEL 2 22.17166667 ll.3d~83333 4.57 ERROR 12 29.8tltl33333 2.49ilb~'t44 UNCORRECTEJ TOTAL 14 52.66000000 R-SQUAil.E c.v. SHl DfV TMT MEAN 0.432428 lit9.2866 1.57819341 1. 05714286 SOURCE: PR > f u.0334 LEAST SQUARES MEANS SOURCE OF TYPE I SS F IIALUE 1 1 15.64571429 7.12595238 6.28 2.86 xo GROUP PARAMEH:l. CONTRAST PR > f o.o21c. 0.1165 PR > ITI ESTIMATE T FOR HO: PARAMETER=O -1.44166667 -1.69 0.1165 STD ERROR Of ESTIMATE OF SUM OF SQUARES MEAN SWUARE MODEL 2 9.2 8666667 4.64333333 ERH.uli 12 24.01333333 2.00111111 UNCORREC Tbl TuTAL 14 33.30000000 R-SQUAkE c.v. sro oev RESIO MEAN o.27&lH9 1414.6063 1.41460634 -0.10000000 SOURCt xu GROUP PARAMETEI< CONTRAST '!$ ·~,t f lfALUI: PR > f 0.1406 F VALUE PR > F 1 1 0.14000000 9.14666667 0.07 ._.57 o. 7959 o.o5n T FOR HO: PARA14ETER=O PR > lT I E:STHlATE STO ERROR OF ESJIMATE 1.63333333 2.14 0.0538 o. 76397474 TYPE I STO ERR t.SHEAN PRUS > ITI HO:LSM£AN=O Al_S2 0.23333333 1.67~00000 0.64429476 0.55797563 0.7235 Bl_A2 Gt{OUP RESID LSMEAN STD EHR LSMEAN PROB > ITI HO:LSMEAN=O A1_o2 S1_A2 O.t13333333 -0.80000000 0.57751062 0.50013887 0.1357 GROUP TREND LSMEAN sro ERR PROB > I r& HO:LSMEAN=O Al_B2 B1_AZ 0.23333333 -1.67500000 0.55797563 o.ouo Yl LSMEAN STI> ERR LSMEAN PRUB > ITI HO:LSMEAN=O 0.30000000 -1.23750000 o.5109133.tt 0.44251589 o.56ao 0.0161 o.ouo 0.17~6 LSMEAN 0.64~29476 0.7235 2.32 SS Of TMT LSMEAN o.o52Jll86 DEPENDENT VARIABLE: RESlO SOURCE GRJiJP GR~UP Al_B2 Bl_A2 -76- .. ~-- ~· '· ·.: --:: • s DEPENOE~T • GENERAL LINEAR MODELS PROCEOURE V6~1ABLE: TREND SOURCE OF SUM OF SQUARES MEAN SlollJAKE F 1/ALUE t-IOOEL 2 22.77166667 ll.385d3333 ~.57 ERROR 12 29. 8dti33333 2.4'1J6944~ UNCORRECTcJ TOTAL 14 52.66)00000 R-SQUARE C.IJ. STU OEIJ TREND MEAN 0.432428 184.1226 1. 57819341 -0.85714286 JF- TYPE I SS 1 1 10.28571429 12.48595238 ... u ESTIMATE T FOR HO: PARAMETER=O PR > ITI STD ERROR Of ESTII'IATE 1.90833333 2.24 0.0449 o.a5232186 SOURCE xo 'JROUP PARAMHE~ CONTRAST PR > f o.J33-. f IJALUE PR >F 0.0649 0.0449 5.01 DEPENDENT VARIABLE: V1 OF SUM OF SQUARES MEAN S<lUAkE F VALUE MODEL 2 12.79125000 6.39562500 4.08 ERROR 12 1B.79d75000 l.:to65o250 PR > F UNCORRECTED TOTAL 14 31.59000000 R-SQUAKE C.IJ. STD DEV Yl MEAN 0.404915 21&. 3301 1. 2 5162 395 -0.57857143 t)f TYPE l SS F IJALUE 1 1 4.6db42857 8.10482143 2.99 5.17 ESTIMATE T FOR HO: PARA14ETER=O PR 1.53750000 2.27 SOURCE SOURCE )(0 GROUP PARAHETE R CONTK4ST 0.0444 PR > f 0.1093 0.0421 > IT I STO ERROR Of ES Tl14A TE 0.0421 0.67595419 -79- • :. 0135 'D' ....__, • • 1 2 3 4 5 6 7 & 9 10 11 12 13 l<t 15 16 17 ltl 19 20 21 22 23 24 25 26 21 28 SUBJECT 1 1 2 2 3 3 . 4 5 5 b b 7 1 8 8 9 9 10 10 11 11 12 12 13 13 14 1ft xo ;.>ROUP 1 1 1 1 1 1 1 1 Al_B2 Al_B2 Al_B2 A1_B2 Al_B2 A1_82 A1_82 A1_82 A1_d2 A1_82 Al_B2 Al_B2 6l_A2 B1_A2 dl_A2 B1_A2 Bl_A2 Bl_A2 Bl_A2 o1_A2 B1_A2 tH_AZ B1_A2 B1_A2 IH_A2 81_A2 Bl_A2 .8l_A2 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 PERIOD 1 2 1 2 1 2 1 2 1 2 1 2 1 2 1 2 1 2 1 2 1 2 1 2 1 2 1 2 y TRT 0.2 loO A 0 A B A 8 A o.o -o.a -0.7 0.2 0.6 1.1 0.3 0.4 1.5 B A 8 A B 1.2 B A 1.3 0.9 -2.3 1.0 B A d A o.o 0.6 -O.d -0.3 -0.4 -1.0 -2.9 B A 8 A 8 A B A B A 1.7 -1.9 -0.3 -2.9 0.9 -:.G- -·"":;: .!) • GENER4L LINEAR MODELS PROCEDURE CLASS LEVEL CLASS LEVE:LS VALUES TRT 2 A 8 Pi::RiuO 2 l 2. SUBJECT 14 ukfJUP 2 IN~GKMATIUN 1 2 3 4 5 6 7 d 9 10 11 12 13 • l~ A1_82 B1_A2 NUMdER Of OBSERVATIONS IN DATA SET = 28 DEPENDENT VARIABLE: Y MEAN SOURCE OF SUM OF Sf.IUARES ptOOE L 16 2.d.03583333 1.75.223958 ERROR 12 14.94416667 1.24534722 UNCORRECTEJ TOTAL 28 42.9b000000 R-S~.o~IJARE c.v. STO DE:V 0.652300 22H.9025 l.ll595126 OF TYPE I SS F VALUE xo 1 GROUf> 1 0.06 3.o7 1 .).07000000 4.57333333 12.00666667 7.82285714 3.56297619 SOURCE OF TYPE II SS xo 0 1 12 1 SOURCE SUBJECT! ~.>ROUP I PERIOD JRT GROuP SUB.I EC f I Gt<JUP I PERIOD TRT SOUIR CE xo GROUP SUBJtCTivRCJUt>l PERiUG r~ r 12 1 Y VALUE 1.41 PR > F MEAN -0.05000000 PR > F o.so The TYPe I SS test equality of weighted means for the PERIOD effect. This is probably not of interest. 6o28 2.86 f VALUE PR > f o.oooooooo l 3.67 0.80 5.01 2.86 OF TYPE Ill SS F VALUE 0 O.JOOOOOOO 1 4.57333333 3.67 1 12.00666667 6.24297619 3.56297619 0.30 5. 01 2.86 1 f 0.2780 4.57333333 12.00666667 6.24297619 3.56297619 u Sf.IUARE ~ 0.044'; 0.1165 f'j{ > f ~ o.044<J 0.1165 The Type III SS give the appropriate tests of unwe"'.ghtef1 menn" for t"!:': TRT and PERIOD effects. (See J) , and ESTIM.~.TE on next pa~;e. ' JY -2l- • ~ ~ • • ., TESTS OF HYPOTHESES USING THE TYPE l MS fOR SUBJECTtGROUPl AS AN ERROR TERM JF SOURCE GROUP TYt>E I SS F VALUE 4.57333333 4.57 > f I (Residual) T fOR HO: ESTIMATE PARAME f ER=O 0.72083333 -0.95416667 1.69 -.2.24 PARAMETE.R TRT PERl 00 PR 0.0538 > IT I PR STu ERROR Of tS II MATE HEANS N y A1_82 Bl_A2 12 0.4166666 7 -0.40000000 16 SUBJECT GI(OUP N y l Al_B2 Al_82 Al.:_B2 Al_B2 A1_82 Al_B2 Bl_A2 Bl_A2 Bl_A2 tH_A2 Bl_A2 Bl_A2 B1_A2 B1_A2 2 2 2 0.60000000 -0.35000000 -0.30000000 0.85000000 0.35000000 1.35000000 1.10000000 -0.65000000 0.30000000 -0.55000000 -0.70000000 -0.60000000 -1.10000000 -1.00000000 2 3 4 5 6 1 !l 9 10 11 12 13 14 2 2 2 2 2 2 2 2 2 2 2 PER 100 l 2 TRT A d N y 14 14 -0.51857143 0.4 7857143 N y 14 14 o. 37857143 -0.47851143 ( o.426l6J93 0.42616093 _.) 0.1165 0.0449 GRUUP \ -b2- Compare with parts ]) and :0 . • I I I I + * • I i.•l 'C• I * * + ..... * * * <I') * * I I I I I * * * * + 0.. * * * * 0.. .... * w <I') (~'«) 0 I I * * I I I I I I I I I I * * * * <:>: I l u.. 0 ..... a I I I * 0.. * * I I I I I I I II I I I I I * I I *I I +1'\J I I I ! I I I - - + - - - + - - - + - - - + - - - + - - - + ---·---+-~~~---+---•-1I . 1(\ .... a- 0 • . "" 0 . 0 0 I I I I I * ...j • N . ,., -o a- 0 I 0 I 0 I . . . N 1(\ .... ..... I I I • ~IIK41t~D-------------------------------------3alanced for Residual Effects. DATA cow; INPUT S~UARE COW CARDS; lJ PkCC P~RIOD TRT $ YIELU RA RB RC (p. 134ff in Cochran and Cox, 1957) ~ES; P~INT; VAH SQUARE COW PERluD TRT RA RB RC RES YlELu; TITLE A CROSSOVER OESIG~ wHEN THERE ARE PGSSIBLE RESIJUAL EffECTS; ® PKOC GLM; CLASSES S~UARE COW PERIOD TRT; ~OJEL YIELD = COw PERIODISQUAREJ RA RB RC TRT I SSli ~EANS TRT I UEPONLY; LSMEANS TRT I STDERR; ~ PRGC GLM; CLASSES SQUARE COw PERIOD TRT; MU~El YIELD= COw PERIOOIS~UAREJ TRT RA RB kC I SSli ESTIMATE 'TKT A VS AVGIB+C)' TRT 1 -0.5 -O.Si ESTIMATE 'TRT B VS T~T C1 TRT 0 1 -1; LS~EA~S TRT I STDERK; MEANS TRT I OEPONlY; OUTPUT OUT=NEW PREOICTED=P RESlDUAl=R; ~ PROC PLOT; PLOT R*P= 1 * 1 I VREF=O; A check on the residuals reveals no obvious violations of the assumed model. ® OBS SQUARE 1 l. 3 4 5 SQUARE, COW, PERIOD, TRT and RES are CLASS variables. R~, RB and RC are 0,1 indicator variables which indicate the residual effects of each of the three treat~ent~. YIELD is the response variable, coded milk yield. cow 1 1 1 1 1 1 2 2 2 3 15 1 1 1 1 1 1 2 2 2 2 2 2 lb 2 0 1 d 9 10 11 12 13 14 z 17 18 2 -:.'-- CLASS, indicator and response variables PERIOD l 2 3 1 2 3 TRT A B c B c A c 1 2 A 3 B 4 4 l 2 A 4 3 1 ti i3 2 3 l A c c 2 6 3 A = 3 5 5 5 b b 6 c RA RB RC 0 ;J 1 0 0 0 0 0 0 1 0 1 0 J 0 1 0 0 0 0 0 0 1 i) 0 0 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 l 0 1 0 0 0 1 0 0 0 0 1 0 RES 0 1 2 0 2 3 0 3 l 0 YIELD 38 2.5 15 109 8& 39 124 12 27 1 8& 76 J '+b 0 l. 1 0 3 z 75 35 14 101 63 1 • ~ • I'he model fitting residual effec··fore treat:::ent effects. d A CROSSOVER DESIGN WHEN THERE AKE POSSIBLE RtSlOUAL EFFECTS GENERAL LINEAR ~UOELS P~JCCUURE CLASS LEVEL INFORMATION CLASS LeVELS VALUES S<JUAKE 2 l 2 Cuw & l 2 Pt:IUUu 3 l 2 3 TKT 3 A B C j 4 5 6 NUMBER Of OBSERVATIONS IN IJATA SET TRT = Rougha€e diet B = Li~ited grain diet C = ?ull grain diet A = 1:3 DEPENDENT VARIABLE: YIELD SOURCE Df SUM OF SQUARES MEAN Sf.1UAI{E '400El 13 20163.1'1444444 1551.01495726 ERROR 4 199.25000000 49.81250000 17 20362.44444444 R-SQUAf<E c.v. STO OI:V YIELO MEAN o. <}<}0215 12.0761 7.05779711 5d.44444444 Jr H'PE I SS 5 5781.11111111 ll48'i.lll1llll 11.75555556 2o.66666667 CORRECTED TUTAL SOURCE cow PERIOD I S>.luAf-:[ l RA1 R.B 4 1 1 RC J· 0 TR T 2 z f VALUe PR > f 23.21. 57.66 0.24 0.54 0.0047 0.0009 0.6525 0.5049 28.65 0.0043 I'he SS due to residual effects unadjusted for treatments is found as: ss Rer-id.ual effects (unadj.) = RA + RB + RC 11. 7 556 +26.6667 +0 ~c.:,.22~ VALUE 31.14 PR > f 0.0023 J.OOOOOOOO 2d54.55000000 f >·rith 2 df. -::;- ·;:.,,. '{!t • :s • The JlOd.el-fi tting treatments -oefore . a l effects. A CKOSSGVEK DESIGN WHEN THERE ARE POSSISLE RESIDUAL EFFECTS GENERAL LINEAR ~ODELS PROCEDURE OEPtNDENT Vt..'K !A3LE: Yl ELO SOURCE OF SUM GF SQUARES MEAN SQUARE .''IODEL 13 20163.1'14ft4444 .1:>51. Jl495726 31.14 EKRCR 4 l 9 "· 2 50 00 0 0 0 49.81250000 PR > F 17 20362.44444444 R-SQUAKE c.v. STO OI:V YIELD MEAN 0.990215 12.07ol 7.0577'1711 58.44444444 CDRRECTEJ TOTAL SOURCE cow 5 4 2 1 1 0 PERIGO! SQUAKE I TRT RA} RB RC SS F VALUE 578l.llllllll ll489.llllllll 227o. 77777778 { 258.67361111 357.52083333 0.00000000 23.21 57.66 22.o5 5.19 7.18 TRT A VS AVGIB•C) T~ T C PR PR > ITI -23.93150000 -20.b2500000 -6.07 -4.53 0.0037 0.010b @ Source Cows Periods w/i squares Treatments (unadj.) Residual (adj.) Residual (unadj.) I'rea tmen ts (adj.) Error Corrected Total > F 0.0047 0.0009 0.0065 0.084'1 0.0553 T FOR HO: PAR AMETEk=O Combining the results of L i. I 5 4 2 2 2 2 -~ 17 ss MS 5781.1111 11489.1111 2276.7778' 616.1944 38.4223 2854.5500 199.2500 20362.4444 1138.3889 308.0972 19.1115 1427.2750 49.8125 S 3 Residual effects (adj. ) = RA + RB + RC = 258.6736 + 357. 5202 + 0 = 616.1944 with 2 df. STU EKfWR Of ESTIMATE 7 3.94542853 4.55576644 ; and ]) gives the ANOVA table found on page df VALUE 0.0023 ESTH4AH PARAMETER TRT B VS TYPE DF F -E6- These are contrasts among the unadjusted treatment means. 135 of Cochran and Cox; • • A CROSSOVErt DESIGN HHEN THEkE ARE POSSIBLE RESIDUAL EFFECTS GENEKAL LIN~AR ~OOELS :-lEANS ~MuSSOVER The unadjusted treatment means TRT '\j YIELD A B 6 45.1666667 57.5000000 72.6666667 6 6 c A PRUCEUUKE UESIGN WHEN THERe ARE POSSIBLE KESIDUAL EFFECTS GENERAL LINEAR ~00ELS PRuCEDUME LEAST SQJARES MEANS = TkT '((ELll LSMEAi~ A .. z .4861111 B 56.1111111 76.7361111 c The treatment means adjusted f8r residual effects STD f:RR LSMEAti PROa > lTI HO:LSMEAN=O 3.1121960 3.1121960 0.0002 3.1121960 o.ooo1 0.0001 Notice that the adjustments are intuitively pleasing. -~<7- • • Allen, David M. and Cady, . .JlY..... Regr·essi on. Foster B. ( 1982). Analyzing .E:-:pel~iment,..?..L Lifetime Learning Publications, Belmont, CA. U§.i.~ Aref, Suzanne and Piegorsch, Walter Data by Regression X SAS: ( 1980) . Analyzing Experimental l.f.:!chni cal Annotated outputs. p,:tpt:~r-· BU-705-M in the Biometrics Unit series, Cornell University, Ithac;:,, N\1 • Arneson, Valerie, Firey, Patricia A. and McCulloch, Charles E. Statistical methods X SAS. Brogan, • Donna R. and Kutner, Technical paper BU-664-M in the Michael H. ( 1980) • Comparative analyses of pretest-posttest research designs . Cochran, William G. and Cox, John Wiley, Gertrude M. (195'7). New York. Grizzle, James E. The two-period change-over design and its use in clinical trials. Meredith, Michael P. Annotated SAS Output CASO). Technical paper BU-814-M in the Biometrics Unit series, Cornell University, Ith<::1c:a, N'i'". SAS Institute, InstitutE?, Inc. ( 19B2) • Inc., Car-·y:, 1\!C:, 584 pp. Snedecor, George W. and Cochran, William G. Iowa State University Press, Ames, -88- Iowa.