UNIVERSITY OF ILLINOIS AT CHICAGO
Department of Psychiatry
Fifth Annual Research Forum – Extravaganza 2014
POSTER TITLE
A new method to generate neurons effectively from cultured SH-SY5Y cells
Neuronal production, SH-SY5Y cells, neural stem cells, and condition medium
DISEASE/KEY
WORDS:
AUTHORS:
Hongna Yang, Yan Li, Feng Wang, and Tingyu Qu
MENTEE
CATEGORY:
Post- doc, PhD student,
Visiting scientist
BACKGROUND:
It is well known that the differentiated SH-SY5Y cells can be used as a
neuronal cell model in vitro for neuroscience and neurotoxicity research. Retinoic
acid (RA) is the most commonly used inducer for neuronal differentiation of SHSY5Y cells. However, neuronal production is very low under RA treatment or
combination of RA and other chemicals.
METHODS:
We explored to use a conditioned medium of human neural stem cells (CMhNSCs) to culture SH-SY5Y cells in combination with RA for 3 to 7 days to
produce neuronal cells.
RESULTS:
We found that the neuronal proportion of differentiated SH-SY5Y cells was
significantly iicreased after 3 and 7 day treatment of CM-hNSCs and RA
compared to that with only RA treatment, in which about 90% of differentiated
cells showing positive beta-III tubulin staining, a well-accepted neuronal marker.
In addition, maturation of the neurons differentiated from SH-SY5Y cells
following CM-hNSCs and RA treatment was greatly improved, with long and
multiple cell process as well as fiber development.
CONCLUSIONS:
It is the first study to use CM-hNSCs for promoting neuronal differentiation of RA
treated SH-SY5Y cells. Our new method can effectively induce neuronal
differentiation of SH-SY5Y cells and increase the proportion of neurons in these
differentiated cells. This research is supported by grants of DoD (PR100499P1) &
the Boothroyd Foundation to T. QU.
RESEARCH MENTOR:
Tingyu Qu