Microbial risk assessment for pluvial urban flooding Introduction Public health risk is an aspect of urban pluvial flooding that up till now has received little attention in technical discussions on the subject. In this research, the results of pathogen measurements in sewer systems and an urban flooding experiment are presented and used in the application of an existing risk analysis method for the quantification of infection probabilities. Research objective The objective of the research project is to find a way to apply existing methods for risk quantification to the case of urban flooding, to assess and quantify the health risk via the oral-fecal contamination route of urban flooding and demonstrate the application of this method with data from practice. A comparison to the WHO Guideline for safe recreational water environments is used to give a framework for the magnitude of the risk. water op straat.jpg Method Quantitative Microbial Risk Assessment is chosen to quantify infection risks. The method uses ingested doses of pathogenic organisms for the calculation of an infection probability. In order to estimate this dose wastewater samples have been collected from the sewer system of Utrecht and have been analyzed for concentrations of Campylobacter, Cryptosporidium and Giardia. Probability distributions on ingested doses are used in Monte-Carlo simulations to quantify infection probabilities for flooding events. Results The results show that mean probabilities of obtaining a Campylobacter or Giardia infection as a result of contact with wastewater in urban flooding are for splashed pedestrians respectively 2.8% and 0.6% per event; for playing children these probabilities are higher than respectively 5.7% and 1.0% per event. Infection probabilities for Cryptosporidium are about 1000 times lower than for Giardia. Samples from an actual urban flooding situation show that the calculations may be conservative. When comparing the infection probabilities of urban flooding to the values for ‘acceptable risk’ as defined by the WHO for bathing water, the health risk caused by urban flooding appears to be higher than the risk posed by swimming in recreational freshwater environments. Conclusions and recommendations The health risk posed by exposure to flood water is significant for Campylobacter and Giardia. It is therefore recommendable to avoid the use of urban flooding water for recreational purposes and for drivers to be careful not to splash pedestrians. A case-study for a flood-sensitive area can be performed in order to gain more insight in the epidemiological aspects of the health risk caused by flooding. Student: G. Sterk Committee: Prof. dr. ir. F.L.H.R. Clemens, ir. J.A.E. ten Veldhuis, dr. B.R. Berends (IRAS, University of Utrecht), Prof. dr. ir. N.C. van de Giesen, dr. ir. J. de Koning For more information please contact the section Sanitary Engineering (+31 (0)15 2783347)