Joe, I just found your email in my spam folder. Sorry about the delay. I
agree...My idea was that this chapter would serve as you required paper for
advancement. We really need to get this out for both of our benefits.
Joe, I am concerned wit the pace of your progress. I need to know if you going to
be able to get into consistent data collection mode. We have invested heavily in
time and money and as we do not have a single useful figure. Galya is excited to
get solidify our collaboration and there are tremendous opportunities. At this
point. We need one fluorescent material that is toxic, one that is not, and them
run a comparative study. All is in place to get this done.
Robert
-----Original Message----From: Fisher, Joseph [mailto:fishejos@onid.orst.edu]
Sent: Tuesday, November 02, 2010 2:05 PM
To: Tanguay, Robert; Harding, Anna
Subject: Project and PhD work -- Meeting Update
Robert,
I met with Anna this morning and gave her an update on my Project/PhD
progress and the new direction of using the ACS book chapter writing to
satisfy the PhD project requirement. Anna is OK with the idea but just
reiterated that the PhD project has specific requirements and it appears
that book chapter would only be a subset of the PhD project.
I've
included the PH project requirements and department references at the
bottom of this email.
Here is our outline of the ACS Chapter/Paper and what I propose and/or
think the PhD project should be to fulfill the Public Health
requirements. Your feedback is welcome.
ACS CHAPTER/PAPER
---------------------------------------------------------------------------------------------------------------------[I. Nanomaterial Properties and Characterization (RT: keep it short):
Properties - chem/phy/bio, Characterization, Periodic System, Reference
Nanomaterials, Database of Nanomaterials.]
[II. Zebrafish (RT: main thrust of chapter): Model Vertebrate Organism,
Toxicologically Relevant (and homologous), Endpoints (physical,
behavioral, genetic), Genetics, High Throughput Screening.]
[III. Nanomaterial Biological Interactions (RT: embryo, fry, adult):
Nanomaterial Stock, Exposure Media Stock, Exposure and Dose, End Effects
Assessment, Biological Distribution and Fate.]
[IV. Hazard Identification and Dose Response Assessment (risk =
f(hazard, exposure): ???, Database of End Effects - Distribution - Fate,
NBI Knowledgebase.]
---------------------------------------------------------------------------------------------------------------------Proposed PhD Project: Nanotechnology and Biotechnology Risk Assessment
-- The Zebrafish Model for Rapid Material Testing.
---------------------------------------------------------------------------------------------------------------------Background: Understand the use of zebrafish as an animal model for
nano-bio interactions and high throughput screening.
Problem: There is little data/information on risk with nano-bio
interactions.
Specific Aims 1 (Lit Review): Review literature and report on zebrafish
as a model vertebrate organism.
RQ 1A: Are zebrafish a toxicologically relevant platform for
investigating nano-bio interactions?
RQ 1B: What endpoints (physical, behavioral, genetic) have been or could
be utilized in analyzing zebrafish?
RQ 1C: What added value do high throughput screens provide?
Specific Aim 2 (Data Analysis): Test nano-bio interactions
(dose/response) and fate versus biological effect (mortality, morbidity,
behavior) with zebrafish.
RQ 2A: Do nano-bio interactions (dose/response) correlate with fate and
biological effect?
RQ 2B: Can nanomaterial fate be detected in zebrafish using high spatial
and temporal imaging?
Specific Aim 3 (Risk Assessment): Use results from the literature review
and data analysis to add to the data/information on risk with nano-bio
interactions.
RQ 3A: How can these results of nano-bio interactions be incorporated
into data and knowledge-bases in order to improve hazard identification
and dose response assessment?
RQ 3B: What ontology and/or parameters are needed in zebrafish
experiments and nano-bio informatics systems to answer important
nanomaterial health and safety questions?
Methods:
(Lit Review) -- Literature databases, peer reviewed and gray literature.
(Data Analysis) -- Zebrafish embryos (0 to 5 dpf), 96 well dose/response
experiments, human/machine assays, statistical analysis, imaging.
(Risk Assessment) -- Database, transforming data to information, agent
(nanomaterial properties) and effect data (molecular, cellular, organ,
organism).
---------------------------------------------------------------------------------------------------------------------DISSERTATION PROPOSAL COMPONENTS
1. Nanomaterials -- Occupational Health and Safety aspects and a
Research Laboratory Health and Safety Guidance. (prior plan for PhD
project)
2. Nanotechnology and Biotechnology Risk Assessment -- The Zebrafish
Model for Rapid Material Testing. (proposed plan for PhD project)
3. TBD (Could incorporate nanomaterial work with military, nano-bio
informatics with Tanguay/Harper, HT informatics with Tanguay, or PNNL
nano-bio imaging with Tanguay/Orr).
PH PROJECT REQUIREMENTS
Project Proposal. Students will write a project proposal between 10-20
pages (double-spaced) and obtain approval to proceed with the project
from their major professor and their second committee member. The
project proposal must contain the following: background/literature
review, specific aims/hypotheses/ research questions, and brief
description of methods, including human subjects/ IRB protocol.
Project Report. The project write-up must include the following
components: background, literature review, specific
aims/hypotheses/research questions, methods (qualitative or
quantitative), results and conclusion. The suggested length of the
project write-up is 25 double-spaced pages, however, page lengths may be
further determined by the student's Project Committee. Students are
highly encouraged to submit their near-to-final draft of their report to
peer review with fellow graduate students with respect to obtaining
feedback on the writing of the project report. Specific information
about this activity will be available from the major professor.
REFERENCES
http://www.hhs.oregonstate.edu/ph/environment-safety-health
http://www.hhs.oregonstate.edu/ph/sites/default/files/2010-2011-PhD-Handbook.pdf
-Joe Fisher
Corvallis, OR 97331
831-915-9404 (cell)
Email: fishejos@onid.orst.edu
Tanguay Lab -- Environmental&
Molecular Toxicology