Raw Material(s) for Hybridoma Development Information Form
Instructions
1.
Please complete and mail a hard copy of this form together with your antigen and all additional information to
Antibody Services, GenScript Corp., 860 Centennial Ave, Piscataway, NJ 08854, USA
2.
* Mandatory information
Customer Information
Account number:
Order ID or Quotation ID:
A.
B.
C.
D.
E.
F.
Immunization Antigen Information
Positive screening/purification Antigen Information
Negative screening/purification Antigen Information
Immunization Cell Line Information
Negative Control Cell Line Information
General amount and COA requirements for materials to be submitted
A. Immunization Antigen Information
Type of antigen*:
Soluble Protein
Membrane Protein
Bacteria
Cell line
Antibody
Peptide
Gene-to-Protein-to-Antibody in GenScript
Other:
Carbohydrate
Lipid
Virus
If cell line is selected, please fill target protein information in this form and fill Cell line information table D.
NOTE: If the antigen is recombinant protein, please describe which fusion tag was used. 0.5-1 mg tag alone or
generic protein with the same tag is requested for monoclonal counter-screening or 3 mg for polyclonal antibody
cross-absorption. Otherwise, tag cleaved recombinant protein should be provided.
Is your antigen toxic or harmful to humans/animals?
Yes
No
If Yes, please specify:
Name of Immunization Antigen*:
Species of Immunization Antigen*:
Human
Mouse
Rat
Yeast
E.coli
Does your antigen have known homologs in other species?
Yes
No
If YES, please specify:
Mouse
Rat
Other animal:
Identities rate:
Yes
Is the sequence or molecular structure of the antigen available?
Other:
No
If YES, please include the sequence information:
Molecular Weight*:
If the antigen MW less than 8 kDa, GenScript will request antigen to be conjugated to carrier protein (KLH, BSA, OVA, etc.) to
enhance immune response.
Format (e.g. liquid, lyophilized, gel, etc.) :
How to reconstitute if it’s lyophilized?
Tag or conjugate*:
Buffer components:
Storage/shipping temperature:
Number of vials:
Volume per vial and concentration:
Precautions:
Certificate of Analysis of your material:
Please attach your COA data of your customer antigen. (ex. SDS-PAGE/HPLC/MS for purity/MW/quantity, etc.)
B. Positive screening/purification Antigen Information
Antigen P1.
Type of antigen
Antigen P2.
Antigen P3.
Antigen name
Sequence or molecular
structure*
MW
Format
Tag or conjugate*
Buffer components
Storage/shipping
temperature
Number of vials
Concentration
COA
How to use
(Recommended usage)
Precautions
C. Negative screening/purification Antigen Information
Antigen N1.
Antigen N2.
Antigen N3.
Type of antigen
Antigen name
Sequence or molecular
structure*
MW
Format
Tag or conjugate*
Buffer components
Storage/shipping
temperature
Number of vials
Concentration
COA
How to use
(Recommended usage)
Precautions
D. Immunization Cell Line Information
Name of Cell line*:
Name of Cell line on Vial Label*:
Format:
Frozen (>2 vials of frozen cells, >106 cells/vial)
Number of vials:
Growth Conditions and Media Requirements:
Mycoplasma Test Result:
Positive
Negative
Unknown
Number of cells/vial:
Note: Our default culture medium is DMEM with 10%FBS and IMDM, RPMi can be selected as well. If you require the
special culture medium, an additional fee will be charged.
Target protein information*: Please fill Table A
Certificate of Analysis of your antigen:
The target protein expression level should be >50,000 copies/cell or more than one log shift on flow cytometry assay.
Precautions:
E. Negative Control Cell Line Information
Name of Cell line*:
Frozen (>2 vials of frozen cells, >106 cells/vial)
Number of vials:
Format:
Name of Cell line on Vial Label*:
Mycoplasma Test Result:
Positive
Number of cells/vial:
Negative
Unknown
Cell density/volume:
Growth Conditions and Media Requirements:
Note: Our default culture medium is DMEM with 10%FBS and IMDM, RPMi can be selected as well. If you require the
special culture medium, an additional fee will be charged.
Target protein information:
Precautions:
F. General amount and COA requirements for materials to be submitted
Screen materials
Fusion tag
The purified protein
Cell line
Bacteria/Virus
Activated protein/antibody
Plasmid
Total quantity
> 0.5 mg
> 0.5 mg
> 106 cells
> 108 clones
> 0.5 mg
>50µg
WB Test materials
The purified protein
The transfected cell
Cell lysate
Tissue lysate
Total quantity
10µg
>1 mg total protein
>1 mg total protein
>1 mg total protein
Total concentration
>0.4 mg/ml
>0.4 mg/ml
>108 /ml
>0.4 mg/ml
Total concentration
>0.5 mg/ml
>2 mg/ml
>2 mg/ml
>2 mg/ml
COA (Not limited)
WB
SDS-PAGE
FC/WB/ICC
Inactivation
Activity assay
Sequencing/vector
COA (Not limited)
SDS-PAGE
WB/FC
Project Information
Is this project for grant application purposes?
When will the project start?
Immediately
Within one month
Yes
No
Within 3 months
Half a year or more