BIOT
DIVISION OF BIOCHEMICAL TECHNOLOGY
Final Program, 234th ACS National Meeting, Boston, MA, August 19-23,
2007
W. Chen, W. Wang, and A. S. Rathore, Program Chairs
SUNDAY MORNING
Section A
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Stem Cells: Quantifying, Modeling and Controlling Stem Cell Fate
J. M. Piret, Organizer
8:20 —1. Behavior-activated cell selection: Application to stem cell expansion. B.
Dykstra, J. Ramunas, D. Kent, C. J. Eaves, E. Jervis
8:50 —2. Tracking stem cell fate using a cell-based microscale platform. T. G.
Fernandes, S. J. Kwon, M -Y. Lee, M. M. Diogo, C. Lobato da Silva, J. M. Cabral, J. S.
Dordick
9:05 —3. Exploiting the synergy between lysate and acoustic standing wave fields to
increase the retroviral transduction of suspension cells. P. R. Beauchesne, V. S. Tayi, B.
D. Bowen, J. M. Piret
9:20 — Intermission.
9:40 —4. Self-assembly of multipotent adult progenitor cells (MAPCs) and
differentiation to the hepatic lineage. K. Subramanian, K. Pauwelyn, C. Verfaillie, W -S.
Hu
9:55 —5. 3-D differentiation model of ex vivo megakaryopoiesis. Y. Leysi-Derilou, N.
Pineault, C. Duchesne, J -F. Boucher, A. Garnier
10:10 —6. P53 tumor suppressor protein affects hematopoietic stem cell differentiation
in the megakaryocyte compartment. P. G. Fuhrken, P. Apostolidis, S. Lindsey, W. M.
Miller, E. T. Papoutsakis
10:25 —7. Differentiation of adult human stem cells guided by mechano-sensing of
matrix elasticity. F. Rehfeldt, A. J. Engler, D. E. Discher
Section B
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Biophysical and Biomolecular Symposium: Protein Stability
E. J. Fernandez, N. Rathore, C. R. Middaugh, A. Blake-Haskins, and A. Blake-Haskins,
Organizers
8:00 — Introductory Remarks.
8:05 —8. Use of biophysical techniques for manufacturability assessment of therapeutic
proteins. C. Li, Y. Jiang, J. Svitel, J. Wen, R. Ramachander, S. Wang, J. Li, L. Narhi
8:25 —9. A novel bioseparation approach: Use of chaotropic agents for HIC and ion
exchange chromatography to dissociate noncovalent multimers during purification of an
Fc fusion protein. A. B. Magill II
8:45 —10. Aggregation of a monoclonal antibody induced by adsorption to microparticle
surfaces. J. Bee, J. Stevenson, K. Chatterjee, E. Freund, J. F. Carpenter, T. W. Randolph
9:05 —11. Assessment of protein stability during freeze-thaw process. K. Gupta, N.
Rathore, L. Barron, W. Ji, F. Jameel, K. Murphy
9:25 — Intermission.
9:45 —12. Identifying optimal solution conditions for high concentration protein
formulations: Use of self-interaction chromatography. M. C. Manning, C. S. Henry, J. J.
Valente, R. W. Payne, W. W. Wilson
10:05 —13. Stability of high concentration rhuMAb VEGF. H. Liu, M. E. M. Cromwell
10:25 —14. Stability of protein antigens in vaccine delivery formulations. J. D. Chesko,
J. Kazzaz, P. Malyala, K. Patton, M. Ugozzoli, D. O'Hagan, M. Singh
10:45 —15. Tungsten, prefilled syringes and protein aggregation. R. Swift, Y. NashedSamuel, W. Liu, L. Narhi, J. Davis
11:05 — Concluding Remarks.
Section C
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Biophysical and Biomolecular Symposium: Protein Folding & Characterization
K. Mallela and T. Cellmer, Organizers
8:00 —16. Hidden folding intermediates in small proteins: Implications for the folding
energy landscape, cooperativity, and evolution of protein structures. Y. Bai
8:25 —17. Electrostatic properties of the unfolded state: What can we learn from
modeling? N. C. Fitzkee, B. García-Moreno E
8:45 —18. Ultrafast folding of the villin subdomain. T. Cellmer, W. A. Eaton, J.
Hofrichter
9:05 —19. Simultaneous and systematic evaluation of multiple protein refolding
conditions. P. A. Leland
9:25 — Intermission.
9:45 —20. Driving forces for protein folding on the ribosome as a function of
polypeptide chain elongation: Experimental and computational approaches. S.
Cavagnero, J. P. Ellis, B. C. Mounce, N. Kurt
10:10 —21. Joining NMR and simulation to capture Alzheimer's Aβ monomer and fibril
structural ensembles. N. L. Fawzi, T. Head-Gordon
10:30 —22. Denaturant and isotopic effects on stability and folding of interleukin-1
receptor antagonist. R. F. Latypov, D. Liu, T. Kohno, D. N. Brems, A. A. Raibekas
10:50 —23. Protein folding principles: Foldons, sequential stabilization, and optional
errors. K. Mallela, S. W. Englander
Section D
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Upstream Processing: Advances in Biocatalysis
P. C. Cirino and L. Sun, Organizers
8:00 —24. A blue fluorescent protein with oxidoreductase activity. K. M. Polizzi, D. A.
Moore, A. S. Bommarius
8:20 —25. A novel laccase/redox mediator system increases space-time yields and
operational stability in dehydrogenase reactions. R. Ludwig, D. Haltrich
8:40 —26. Alginate-derived nanovolume cytochrome P450 microarrays for highthroughput inhibition assays. S. M. Sukumaran, M -Y. Lee, D. S. Clark, J. S. Dordick
9:00 —27. Evolution in reverse: Engineering a xylose-specific xylose reductase. N. Nair,
H. Zhao
9:20 — Intermission.
9:40 —28. Directed evolution of LuxI for enhanced synthesis of OHHL. P. K. R.
Kambam, L. Sun
10:00 —29. Biocatalytic systems for aromatic oxidations. A. M. McIver, S. V. B. J.
Garikapati, T. L. Peeples
10:20 —30. Design of an enzyme-chaperone chimera as a new approach to enzyme
immobilization and self-renaturation. L. M. Bergeron, T. Tokatlian, D. S. Clark
10:40 —31. Protein dissection and shuffling for generating recombined proteins. Y. Chen,
T. Chen, H. Hua, Z. Lin
Section E
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Alan S. Michaels Recovery Award Lecture
W. Zhou, Organizer
11:30 — Introductory Remarks.
11:35 —32. Early steps in protein recovery: The messy part of a clean industry. M -R. A.
Kula
SUNDAY AFTERNOON
Section A
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Stem Cells: Stem Cell Based Tissue Engineering
T. McDevitt, T. McDevitt, and P. W. Zandstra, Organizers
2:00 —33. Generation and characterization of keratinocytes from human embryonic stem
cells. C. M. Metallo, L. Ji, J. J. De Pablo, S. P. Palecek
2:30 —34. Characterization of embryonic stem cells' differentiation into insulinproducing endocrines cells in 3-D cultures. X. Wang, K. Ye
2:45 —35. A novel method to control differentiating human embryonic stem cell (hESC)
aggregate size and composition: Effects on cardiomyocyte development. C. L. Bauwens,
R. Peerani, S. Niebruegge, K. Kolodziejska, M. Husain, P. W. Zandstra
3:00 — Intermission.
3:15 —36. Serum-free expansion of embryonic stem cells in a bioreactor for tissue
engineering applications. D. E. Kehoe, L. T. Lock, E. S. Tzanakakis
3:30 —37. Hypoxic effect on hematopoietic development in human embryonic stem cellderived embryoid bodies. C. M. Cameron, W -S. Hu, D. S. Kaufman
Section B
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Downstream Processing: Primary Recovery - Membranes and Other Technologies
D. J. Roush and Y. Lu, Organizers
2:00 — Introductory Remarks.
2:05 —38. Engineering the adsorption of plasmid DNA. W. J. Kelly, P. Butts
2:25 —39. Creating a robust and reproducible recovery process using centrifugation for
the recovery of a soluble fusion protein in Saccharomyces cerevisiae. K. Herzog, J.
Barringer, C. Brady
2:45 —40. Ideal membrane cascades for downstream processing. E. N. Lightfoot, J. L.
O'Dell
3:05 — Intermission.
3:15 —41. Optimization of cell culture harvest clarification using an ultrascale down
model for centrifugation. M. Menon, A. Hamilton, F. Riske
3:35 —42. Effect of solid ejection size on continuous centrifugation during mammalian
cell culture product recovery. X. Zhao, J. Zhou
3:55 —43. Case study: Optimization of harvest stream clarification steps of a
commercially-relevant fusion protein mammalian cell culture using charged depth filters.
R. Bates, J. Ma, C. Mason
Section C
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Biophysical and Biomolecular Symposium: Protein Folding & Characterization
K. Mallela and T. Cellmer, Organizers
2:00 —44. All-atom computer simulation of amyloid fibrils disaggregation. C. Tan, H -F.
Chen, R. Luo
2:25 —45. Dynamics of intramolecular contact formation in islet amyloid polypeptide
(IAPP). S. Vaiana, W. A. Eaton, R. Ghirlando, J. Hofrichter
2:45 —46. Folding of the β-helix domain of P22 tailspike protein. M. L. Spatara, C. J.
Roberts, A. S. Robinson
3:05 — Intermission.
3:15 —47. Folding and insertion of melittin in membrane mimics. M. R. Hartings, H. B.
Gray, J. R. Winkler
3:35 —48. Understanding the molecular mechanism of Hsp90: The role of nucleotide
hydrolysis in the chaperone cycle. K. A. Krukenberg, F. Foerster, A. Sali, D. A. Agard
3:55 —49. Breaking the symmetry of a homodimeric enzyme: Engineering and
characterization of monomeric disulfide isomerases. S. A. Arredondo, G. Georgiou
Section D
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Emerging Technologies: Nanobiotechnology
Cosponsored with ANYL, and BIOHW
J. R. Cochran and L. Malmberg, Organizers
2:00 —50. Co-assembly of genetically-modified bacteriophages and inorganic
nanoparticles into silica-stabilized nanoarchitectures. C. E. Ashley, E. C. Carnes, L. T.
White, Z. Yuan, D. R. Dunphy, D. N. Petsev, P. Atanassov, D. Peabody, C. J. Brinker
2:20 —51. Biofabrication with genetically modified viral nanotemplates. H. Yi, G. W.
Rubloff, G. F. Payne, W. E. Bentley, J. N. Culver
2:40 —52. Engineering stability and self-assembly in a caged protein scaffold. S. Lim, M.
Dalmau Mallorqui, C. I. Ruiz, S -W. Wang
3:00 — Intermission.
3:10 —53. Nanoscale reservoirs for spatially and temporally controlled biointerfaces. N.
A. Melosh, J. J. VanDersarl, E. Hagar-Barnard, E. Yenilmez
3:30 —54. Biological fabrication of nanostructured silicon-germanium photonic crystals
possessing unique photoluminescent and electroluminescent properties. G. L. Rorrer, C.
Jeffryes, D. Lee, T. Qin, C -H. Chang, T. Gutu, J. Jiao, R. Solanki
3:50 —55. Biologically programmed synthesis of hybrid semiconductor nanocrystals. S.
Singh, N. Myung, A. Mulchandani, W. Chen
Section E
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Commercialization of Biologics: Case Studies: Post-Approval Changes &
Comparability Challenges
S. Lee and V. Paradkar, Presiding
2:00 — Introductory Remarks.
2:05 —56. Post approval changes for a commercial process: An historical look. C. Heath,
H. Madani
2:25 —57. Case study: Downstream purification support of a cell culture raw material
change for a licensed mAb product. F. Maslanka
2:45 —58. Rituximab post approval changes and comparability assessments. R. Kiss, S.
R. Chary, S. Meier, J. C. Goodrick, D. C. Andersen
3:05 — Intermission.
3:15 —59. Demonstrating comparability for post approval CMC changes. R. Harris
3:35 —60. Comparability protocol to support a chromatography resin change. P. J.
Kramer, R. Frankenberg, R. Bhat, P. Burd, A. Hetherington
3:55 —61. Investigation of an ultrafiltration yield problem for a conjugate vaccine. S -M.
Lee, B. Kruse, A. Robinson, J. Davis, C. Donaldson
4:15 — Concluding Remarks.
Section F
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Perlman Lecture
W. Zhou, Organizer
4:30 —62. Prion proteins and how they fold. S. Lindquist
Chemical Approaches to Protein Function
Sponsored by BIOL, Cosponsored with ANYL, BIOT, MEDI, BTEC, and BIOHW
MONDAY MORNING
Section A
Unknown Site -- Unknown Room
Emerging Technologies: Nanobiotechnology
Cosponsored with ANYL, and BIOHW
J. R. Cochran and L. Malmberg, Organizers
8:00 —63. Inhibiting bacterial biofilm formation by self-assembled monolayers of
functional alkanethiols on gold. S. Hou, E. A. Burton, K. A. Simon, D. Blodgett, Y -Y.
Luk, D. Ren
8:20 —64. Development of electrospun scaffolds for application to medical devices. R.
Delaporte, H. Yesilalan, R. Skowyra, G. Cadd
8:40 —65. Polymersome encapsulated hemoglobin: A novel type of hemoglobin-based
oxygen carrier. A. F. Palmer
9:00 —66. A novel, biomimetic hydrogel construct to repair the cornea: Molecular design
and biological response. D. Myung, J. R. Cochran, J. Noolandi, C. N. Ta, C. W. Frank
9:20 — Intermission.
9:35 —67. Multifunctional titanium-binding peptides for rapid modification of implant
surfaces. X. Khoo, D. J. Kenan, M. W. Grinstaff
9:55 —68. Effects of platinum and gold nanoparticles on osteoblast cells: A study of
nanoparticles' cancer treatment potential. Y. Sun, D. Katz, T. Fraczak, N. Pernodet, M.
Rafailovich, J. Sokolov
10:15 —69. Degradable nanoparticle gene carriers for efficient and versatile nonviral
gene delivery. S. Lu, Y. J. Kwon
10:35 —70. Assembly of PEI/DNA nanoparticles for gene delivery by microfluidic
hydrodynamic focusing. C -G. Koh, Z. Fei, J. Guan, Y. Xie, L. J. Lee
Section B
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Downstream Processing: Advances in Chromatography
A. M. Lenhoff and S. Ghose, Organizers
8:00 —71. Are high capacity and high selectivity mutually exclusive? New approaches to
the chromatographic resin game. J. Rasmussen
8:20 —72. Microstructure and retention properties of dextran-grafted agarose media. H.
Koku, B. D. Bowes, A. M. Lenhoff
8:40 —73. Use of displacement chromatography for proteomic applications. S. T. Evans,
A. S. Freed, M. Platt, S. M. Cramer
9:00 —74. High performance chromatofocusing as a novel analytical method to support
process comparability and product characterization. X. Kang, J. Kutzko, M. Hayes, D.
Frey
9:20 — Intermission.
9:40 —75. Membrane adsorber technology for trace impurity removal applications. M.
W. Phillips, C. Teske, R. Specht, M. Kozlov, W. Moya
10:00 —76. Validation of host cell protein removal in purification processes of
biopharmaceutical manufacturing. C. Jiang, J. Ma, M. Rubacha, S. Lee, A. A. Shukla
10:20 —77. Profiling metal binding E. coli proteins: Its use in purification process
development. R. Varakala, R. Haley, R. Henry, M. M. Ataai, R. R. Beitle
10:40 —78. Affinity adsorption of viruses using small peptide ligands. C. L. Heldt, P. V.
Gurgel, L -A. Jaykus, R. G. Carbonell
Section C
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Upstream Processing: Advances in Microbial Fermentation Process Development
D. Ren, M. Laird, and X. Yang, Organizers
8:00 — Introductory Remarks.
8:10 —79. Alleviation of extracytoplasmic stress for improving cell physiology and
recombinant protein production in Escherichia coli. C. P. Chou
8:30 —80. Metabolic activities of novel inducible fermentations for recombinant
therapeutic proteins. M. Berge, R. Georgescu
8:50 —81. Fast track first-in-human development of novel therapeutic proteins with a
robust microbial fermentation platform. X. Yang, B. Pendleton
9:10 —82. Using the two population theory to design an inducer feeding schedule to
maximize recombinant DNA protein expression in Escherichia coli. J. M. Gonzalez, J. T.
Hsu
9:30 —83. Mixture design for optimization of fermentation medium for cutinase
production from Colleotrichum lindemuthianum. V. Shah, F. J. Rispoli
9:50 —84. Efficient antibody production with suppressing O-Glycosylation in yeast. K.
Kobayashi, K. Kuroda, H. Tsumura, T. Komeda, Y. Chiba, Y. Jigami, K. Ichikawa, K.
Nonaka, T. Suzuki
10:10 — Concluding Remarks.
Section D
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Upstream Processing: Advances in Biocatalysis
P. C. Cirino and L. Sun, Organizers
8:00 —85. Efficient synthesis of simvastatin using whole-cell biocatalysis. Y. Tang, X.
Xie
8:20 —86. Enzyme-carbon nanotube conjugates in room temperature ionic liquids. B.
Eker, P. Asuri, S. Murugesan, R. J. Linhardt, J. S. Dordick
8:40 —87. Exploiting phenotypic diversity through global transcription machinery
engineering. D. Klein-Marcuschamer, G. Stephanopoulos
9:00 —88. High-throughput zcreening for methionyl-tRNA synthetases that enable
residue-specific incorporation of noncanonical amino acids into recombinant proteins in
bacterial cells. T. H. Yoo, D. A. Tirrell
9:20 — Intermission.
9:35 —89. What controls enantioselectivity for carbonyl reductase. L. Hua, D. Zhu, Y.
Yang, T. H -Y. Pan
9:55 —90. Hydration effects on the active site and catalytic performance of organicsoluble subtilisin in octane. E. P. Hudson, J. S. Dordick, J. A. Reimer, D. S. Clark
10:15 —91. Synthesis of anti-obesity stilbene derivatives using engineered microbial
biocatalysts: Structure-function analysis of -glucosidase inhibition potency. E. Leonard,
K -H. Lim, C -G. Lim, J. Chemler, M. Koffas
10:35 —92. Biotransformations on solid-supported substrates. S. Brooks, L. Coulombel,
U. Akbar, D. S. Clark, J. S. Dordick
Section E
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Industrial Biotechnology Award Lecture
W. Zhou, Organizer
11:30 —93. Genentech Lucentis® Development CMC team: "A vision saving therapy
made by biotechnology". J. C. Joly
Genomics of Obesity
Sponsored by BIOHW, Cosponsored with AGFD, BIOL, BIOT, MEDI, and BTEC
Pfizer Award Symposium
Sponsored by BIOL, Cosponsored with ANYL, BIOT, MEDI, BTEC, and BIOHW
MONDAY AFTERNOON
Section A
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Stem Cells: Engineering the Embryonic and Adult Stem Cell Niche
A. Khademhosseini and B. Rao, Organizers
3:00 —94. Matrix and environmental control of stem cell outcomes for tissue engineering.
D. L. Kaplan, H. J. Kim, X. Wang, X. Wang, C. Vepari, A. Murphy, I. Georgakoudi
3:30 —95. The effect of cell density and oxygen level on culture for rodent multipotent
adult progenitor cells (MAPCs). Y. Park, F. Ulloa, C. Verfaillie, W -S. Hu
3:45 —96. cAMP induced neural differentiation of mesenchymal stem cells: Real
differentiation or pseudo-differentiation? L. Zhang, C. Chan
4:00 —97. Effects of scaffold porosity and pore size on cell entrapment, attachment,
distribution and proliferation in 3-D cell culture system. R. Ng, S -T. Yang
4:15 — Intermission.
4:30 —98. Ex vivo expansion of human hematopoietic stem/progenitor cells in a human
stromal-based culture system. C. Lobato da Silva, R. Goncalves, M. Lemos, F. Lemos,
G. Almeida-Porada, J. Cabral
4:45 —99. Multiparameter flow cytometric assay to monitor the activation of cytokine
signaling pathways in specific subsets of primary erythroid cells. W. Wang, J. Audet
Section B
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Downstream Processing: Bioprocess Integration & Industrial Case-Studies
M. R. Ladisch and C. Liu, Organizers
3:00 —100. CSAF: Constant shear affinity filtration technology. P. Francis, E. von
Lieres, F. Taghipour, B. D. Bowen, C. A. Haynes
3:20 —101. Production of enantiopure molecules by integration of SMB technology and
biocatalysis. M. Bechtold, S. Makart, S. Panke
3:40 —102. Considerations for large scale extraction of monoclonal antibodies targeted
to different subcellular compartments in transgenic tobacco plants. S. Hassan, C. van
Dolleweerd, J. Ma, W. Liu, C. R. Thomas, E. Keshavarz-Moore
4:00 — Intermission.
4:20 —103. Evaluating and minimizing water usage and wastewater ceneration in
integrated bioprocesses using process simulation and scheduling tools. D. Petrides, C.
Siletti
4:40 —104. Development and implementation of a manufacturing scale virus filtration
step for monoclonal antibody production. J. F. Hsii, C. Dowd
5:00 —105. Humira after launch: Opportunities and challenges in downstream process.
M. Wan, F. Gaibor, M. Schrader, R. M. Boychyn, E. Lundell, G. Avgerinos
Section C
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Upstream Processing: Advances in Microbial Fermentation Process Development
X. Yang, M. Laird, and D. Ren, Organizers
3:00 — Introductory Remarks.
3:05 —106. "Seeing the light" with cell-free protein synthesis. A. R. Goerke, A. M.
Loening, S. Gambhir, J. R. Swartz
3:25 —107. Enhancing extracellular protein secretion in Escherichia coli by translation
engineering. P. Gupta, P. S. Lee, K. H. Lee
3:45 —108. Developing high throughput screening methods for multiple phenotype
evaluation. B. Wang, Y. Zhang, G. N. Stephanopoulos
4:05 —109. Metabolic and process engineering of Propionibacterium acidipropionici for
enhanced propionic acid fermentation. A. Zhang, S -T. Yang
4:25 —110. Production of sophorolipid from cooking oil waste through fermentation. V.
Shah
4:45 — Panel Discussion.
5:30 — Concluding Remarks.
Section D
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Commercialization of Biologics: Data Management & Process Modeling in Support
of Commercial Processes
J. Prior and S. Ahuja, Organizers
3:00 —111. Multivariate analysis of transition analysis calculations applied to evaluate
trends and changes in chromatography column packed bed integrity at BiogenIdec. B.
Gilbert
3:20 —112. Application of multivariate analysis toward biotech processes: Case study of
a cell-culture unit operation. A. S. Rathore, A. Kirdar
3:40 —113. Production planning, scheduling, and debottlenecking practices in the
biopharmaceutical industries. C. Siletti, D. Petrides
4:00 — Intermission.
4:20 —114. Case study: Applying the Six Sigma methodology to a commercial cell
culture process. R. Davis, S. Ahuja, M. Herwig, K. Hwang
4:40 —115. Strategies for integrating spectral data from raw materials to control
biopharmaceutical manufacturing. J. Wei, M. Kiistala, M. Lanan, J. Ganguly
5:00 —116. Use of mathematical models to determine the effect of various raw materials
on mammalian cell culture performance at commercial-scale. S. Jain, B. Schilling, A. A.
Shukla, S. Lee
Section E
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Industrial Keynote Symposium
W. Chen, Organizer, Presiding
2:00 —117. Keynote Address. B. Adamson
Genomics of Obesity
Sponsored by BIOHW, Cosponsored with AGFD, BIOL, BIOT, MEDI, and BTEC
Nano-Biochemistry and Technology
Sponsored by ANYL, Cosponsored with BIOT, BIOHW, BTEC, and BIOL
Undergraduate Research Poster Session
Biochemistry
Sponsored by CHED, Cosponsored with BIOT, SOCED, and BIOL
TUESDAY MORNING
Section A
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Emerging Technologies: Bioenergy
M. R. Ladisch and C. E. Wyman, Organizers
8:00 —118. Commercialization of biomass processing: A consolidated approach. M.
Moniruzzaman
8:20 —119. Comparative hydrolysis, fermentation, and economic information for
application of leading pretreatment technologies to corn stover and poplar. C. E. Wyman,
B. Yang, B. E. Dale, R. Elander, M. Holtzapple, M. R. Ladisch, Y. Y. Lee, C. Mitchinson,
J. N. Saddler
8:40 —120. Mechanisms of plant cell wall deconstruction as a function of pretreatment
and enzyme hydrolysis. M. Zeng, N. Mosier, C. E. Wyman, M. R. Ladisch
9:00 —121. Efficient ethanol production with engineered microorganisms. C. T. Trinh, F.
Srienc
9:20 — Intermission.
9:40 —122. Two-stage continuous fermentation of cellulose to ethanol. C. Liu, J.
Bardsley, C. E. Wyman
10:00 —123. Butanol fermentation by Clostridium acetobutylicum immobilized in a
fibrous bed bioreactor. W -L. Chang, S -T. Yang
10:20 —124. Enzymatic synthesis of biomass-based fuel methanol. P. Wang, F. S.
Baskaya, M. C. Flickinger
10:40 —125. Long-term hydrogen production from robust nongrowing
Rhodopseudomonas palustris coatings and strategies for increased hydrogen production.
J. L. Gosse, B. J. Engel, A. S. Al-Homoud, C. S. Harwood, M. C. Flickinger
Section B
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Downstream Processing: Advances in Chromatography
A. M. Lenhoff and S. Ghose, Organizers
8:00 —126. Post Protein A removal of contaminants from monoclonal antibodies with a
multimodal anion exchanger. K. Eriksson, A. Ljunglöf, G. Rodrigo
8:20 —127. Removal of HMW species using AEX in the weak partitioning mode for
antibodies and related proteins. S. A. Tobler, A. Noyes, P. R. Brown, M. Switzer, B.
Kelley
8:40 —128. Advances in membrane affinity chromatography for the recovery of
antibodies. C. Boi, S. Dimartino, G. C. Sarti
9:00 —129. The use of modeling tools for the efficient purification process development.
D. Dong, T. Gervais, D. Tran, N. Quinlan, A. Velayudhan, P. Alred, P. Alfonso
9:20 — Intermission.
9:40 —130. Modeling-based rational approach to purify proteins: A case study. B. K.
Nfor, T. Ahamed, M. Ottens, E. J. A. X. van de Sandt, M. H. M. Eppink, G. W. K. van
Dedem, L. A. van der Wielen
10:00 —131. Investigation of protein binding in ion exchange systems with protein
charge ladders and homologous protein libraries. W. K. Chung, Y. Hou, J. J. Keba, G.
Makhatadze, S. M. Cramer
10:20 —132. Analysis and prediction of protein adsorption isotherms from interaction
data. X. Xu, A. M. Lenhoff
Section C
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Biophysical and Biomolecular Symposium: Protein Chemical Instability
E. M. Topp and M. Cromwell, Organizers
8:00 — Introductory Remarks.
8:05 —133. Chemical stability of proteins in injectable drug delivery systems. F. Okumu
8:25 —134. Fragmentation within human IgG1 hinge region: Two distinct mechanisms,
two different outcomes. J. Vlasak, C. E. Price, S. L. Cohen
8:45 —135. Gamma irradiation-induced damage in proteins and peptides. S -X. Xie, T. D.
Williams, D. Willey, E. M. Topp
9:05 —136. Impact of degradations on bioactivity: A reflection from a monoclonal
antibody. B. L. Yu, A. Vizel, M. Young, A. Morando, B. He
9:25 — Intermission.
9:45 —137. Selective protein oxidation through reactive oxygen species: Catalysis by
cysteine. C. Schoneich
10:05 —138. Using solution NMR to probe the effects of excipients on protein stability.
J. S. Laurence
10:25 —139. Effect of residual moisture content on the stability of dried Apo2L/TRAIL
crystals. H. Flores, A. S. Ressurreiçao, B. Zhang, B. Kabakoff, Z. Shahrokh, M. E. M.
Cromwell
10:45 —140. Protein stability at solid substrates: Influence of chemical and thermal
excursions. G. Anand, G. Belfort
Section D
Unknown Site -- Unknown Room
Upstream Processing: Advances in Metabolic Engineering
J. March and I. S. Aldor, Organizers
8:00 — Introductory Remarks.
8:05 —141. Application of metabolic flux and logarithmic sensitivity analysis for
optimization of A. niger medium. R. Gheshlaghi, J. M. Scharer, M. Moo-Young
8:25 —142. Proteome map of Aspergillus nidulans during osmoadaptation. Y. Kim, M. P.
Nandakumar, M. R. Marten
8:45 —143. Nanofactories for synthesis and delivery of signaling molecules: A tool for
engineering metabolism. R. Fernandes, C -Y. Tsao, C. W. Yung, W. E. Bentley
9:05 —144. Engineering signal transduction for treating type 1 diabetes. J. March, F.
Duan
9:25 — Intermission.
9:45 —145. Development of a melanin-based screen for tyrosine production in
Escherichia coli. C. N. S. Santos, G. N. Stephanopoulos
10:05 —146. Engineering synthetic pathways for production of butanol as biofuels. S.
Atsumi, J. C. Liao
10:25 —147. Intracellular enzyme crosslinking: Toward multifunctional enzyme
machines for the production of R-1,2-propanediol. R. J. Conrado, M. DeLisa
10:45 —148. Toward microbial synthesis of glucaric acid. T. S. Moon, A. Lanza, L.
Octavio, K. Jones Prather
Section E
Unknown Site -- Unknown Room
Marvin J. Johnson Award Lecture
W. Zhou, Organizer
11:30 —149. Molecular bioprocessing from design to discovery to dreams. J. S. Dordick
Genomics of Obesity
Sponsored by BIOHW, Cosponsored with AGFD, BIOL, BIOT, MEDI, and BTEC
RNA Interference Based Therapeutics
Sponsored by CARB, Cosponsored with BIOL, BIOT, COMP, MEDI, ORGN, PMSE,
POLY, and BTEC
TUESDAY AFTERNOON
Section A
Unknown Site -- Unknown Room
Emerging Technology Keynote Lecture
H. Zhao and R. Srivastava, Organizers
2:00 — Introductory Remarks.
2:05 —150. Energy from biomass: Anticipating a revolution. L. R. Lynd
Section B
Unknown Site -- Unknown Room
Upstream Processing: Advances in Cell Culture Process Development
S. T. Sharfstein, I. Blumentals, and G. Maheshwari, Organizers
3:00 — Introductory Remarks.
3:05 —151. Leveraging process characterization and validation data for continuous
process improvement. M. Luu, R. Kiss, S. Meier
3:25 —152. Inhibitory threshold values of lactate, ammonia, carbon dioxide, and
osmolality in CHO cell culture for a therapeutic protein production. Z. Xing, Z. Li, V.
Chow, S. S. Lee
3:45 —153. Scale-up of mammalian cell cultures controlled by automated flow cytometry.
G. Sitton, F. Srienc
4:05 — Intermission.
4:20 —154. Metabolic process engineering applied to cell culture production processes.
B. M. Schilling, A. A. Shukla, S. Lee
4:40 —155. Investigating limitations during process development at reduced perfusion
rates. H. Drouin, S. Lanthier, A. Kamen, J. M. Piret, Y. Durocher
5:00 —156. High-throughput cell culture process development using 3-D
microbioreactors and online quantifications of cell growth and GFP expression. X.
Zhang, Y. Wen, S -T. Yang
5:20 — Concluding Remarks.
Section C
Unknown Site -- Unknown Room
Downstream Processing: Molecular Interactions and Modeling Approaches
J. Thommes and K. M. Lacki, Organizers
3:00 —157. Custom chromatography: Optimizing surface functionalization to meet
specific separation needs. C. A. Haynes
3:20 —158. Molecular dynamic investigation of the interaction of supported affinity
ligands with monoclonal antibodies. L. Zamolo, V. Busini, D. Moiani, D. Moscatelli, C.
Cavallotti
3:40 —159. Construction and study of porous polymer media for ion-exchange
adsorption by molecular dynamics modeling and simulations. E. Riccardi, J. -C.Wang, A.
I. Liapis
4:00 — Intermission.
4:20 —160. Investigation of ligand-protein binding affinity in ion exchange
chromatography. T. Yang, W. K. Chung, S. A. McCallum, J. Kempf, S. M. Cramer
4:40 —161. Molecular level inspection of viral clearance and membrane adsorbers. W. T.
Riordan, M. R. Etzel
5:00 —162. Modeling of protein monomer/aggregate separation using Hydrophobic
Interaction Chromatography: Effects of column scale. J. T. McCue
Section D
Unknown Site -- Unknown Room
Commercialization of Biologics: Impact of Design Space Philosophy & Emerging
Regulations on Process Validation
G. Knipp, K. Webber, and A. Kataria, Organizers
3:00 —163. Efficient approach to setting validation acceptance criteria for a
biopharmaceutical process. T. Gleason, R. Burdick, S. Rausch, J. E. Seely
3:20 —164. A systematic method for design space definition. R. D. Hegedus
3:40 —165. Apply design space philosophy in the establishment of process validation
parameters and acceptance criteria. X. Wang, J. Harms, P. Van Hoek, A. S. Rathore
4:00 — Intermission.
4:20 —166. Quality by design and process analytical technology for freeze drying. M.
Pikal
4:40 —167. Critical process parameters and design space. P. W. Wojciechowski
5:00 —168. Use of online Process Analytical Technology (PAT) to monitor
Maytansinoid conjugation to modified antibodies: Implications in establishing robust
processes for manufacturing disulfide-linked immunoconjugates. I. M. Schwartz, S.
Gohain, D. C. Pentia, G. Amphlett, D. H. Meshulam, G. Malin
Section E
Unknown Site -- Unknown Room
Upstream Processing: Advances in Metabolic Engineering
J. March and I. S. Aldor, Organizers
3:00 —169. Incorporation of thermodynamics and metabolomics data into constraintbased model of Methylobacterium extorquens AM1. R. E. Osterhout, M. van der Werf,
S. Van Dien
3:20 —170. Metabolic engineering of natural and unnatural flavonoid biosynthesis in
microorganisms and their application for diabetes treatments. J. Chemler, M. Koffas, E.
S. Tzanakakis, Y. Yan, E. Leonard, Z. Fowler, L. T. Lock
3:40 —171. Metabolic engineering of Saccharomyces cerevisiae for enhanced uptake and
sequestration of arsenic. M. Shen, S. Singh, D. Shah, W. Lee, W. Chen, N. A. Da Silva
4:00 —172. Metabolic flux elucidation for large-scale models: 13C labeled isotope
experiments and experimental design. P. F. Suthers, A. P. Burgard, M. S. Dasika, F.
Nowroozi, S. Van Dien, J. D. Keasling, C. D. Maranas
4:20 — Intermission.
4:30 —173. Biosynthesis of fosfomycin. Z. Shao, R. Woodyer, H. Zhao
4:50 —174. Probing the supply-side attributes of different metabolically engineered host
backgrounds. D. Cunningham, N. Domagalski, R. Koepsel, M. M. Ataai, M. M. Domach
5:10 —175. Growth-phase accumulation of poly-3-hydroxybutyrate in Escherichia coli:
A prospectus for continuous production. K. E. Tyo, G. N. Stephanopoulos
5:30 — Concluding Remarks.
Genomics of Obesity
Sponsored by BIOHW, Cosponsored with AGFD, BIOL, BIOT, MEDI, and BTEC
Mechanism of Action of Natural Products
Sponsored by BIOL, Cosponsored with BIOT, MEDI, ORGN, BTEC, and BIOHW
Molecular Imaging
Visualization of Biological Processes at the Molecular Level
Sponsored by BIOL, Cosponsored with ANYL, BIOT, MEDI, BTEC, PHYS, and BIOHW
RNA Interference Based Therapeutics
Sponsored by CARB, Cosponsored with BIOL, BIOT, COMP, MEDI, ORGN, PMSE,
POLY, and BTEC
WEDNESDAY MORNING
Section A
Unknown Site -- Unknown Room
Emerging Technologies: Systems Biology
Cosponsored with BIOHW
C. Chan and C. Cho, Organizers
8:00 —176. Scaffold proteins tune the regulatory properties of protein kinase cascades. J.
W. Locasale
8:20 —177. Molecular systems biology via multiscale modeling and high-performance
computing. J. Purvis, Y. Liu, A. Shih, N. Agrawal, R. Radhakrishnan
8:40 —178. Predicted points of cell-cycle fragility are consistent with known
malfunctions in solid and hematological cancers. S. Nayak, S. Salim, J. D. Varner
9:00 —179. Transcription analysis with gene expression and network connectivity data.
M. P. Brynildsen, L. M. Tran, T -Y. Wu, S -S. Jang, J. C. Liao
9:20 — Intermission.
9:40 —180. Genome-scale metabolic analysis of bacteriophage infected Escherichia coli.
R. Jain, R. Srivastava
10:00 —181. Elucidating and filling gaps in genome-scale metabolic networks. V. Satish
Kumar, M. S. Dasika, C. D. Maranas
10:20 —182. Metabolic network inference based on probabilistic modeling of metabolic
profiles. J. Yoon, K. Lee
10:40 —183. Isotopically nonstationary flux analysis using an elementary metabolite unit
(EMU) framework. J. L. Walther, J. D. Young, M. R. Antoniewicz, H. Yoo, G.
Stephanopoulos
Section B
Unknown Site -- Unknown Room
Biophysical and Biomolecular Symposium: Targeted Delivery of Proteins &
Nucleotides
C. Meares, W. Liu, and A. Blake-Haskins, Organizers
8:00 — Introductory Remarks.
8:05 —184. (R-Ahx-R)4 cell-penetrating peptide enhances the in vivo delivery of
antisense morpholino oligomers. H. M. Moulton, N. Jearawiriyapaisarn, S. Fletcher, B.
W. Neuman, R. Kole, W. Steve, M. Buchmeier, P. L. Iversen
8:25 —185. Mutant glycosyltransferases assist in linking glycoconjugates via glycan
chains: Development of a targeted drug delivery system and contrast agents for MRI. P.
K. Qasba, E. Boeggeman, B. Ramakrishnan
8:45 —186. A new gene delivery method based on yeast cell wall particles. E. Soto, G.
Ostroff
9:05 —187. Reversing the nucleophile and electrophile for covalent affinity capture. D. D.
Singh, M. R. McCoy, Z. Miao, O. L. Hsu, C. Meares
9:25 — Intermission.
9:40 —188. D-galactose receptor-targeted in vivo spectral fluorescence molecular
imaging of peritoneal metastasis. H. Kobayashi, Y. Hama, A. J. Gunn, Y. Koyama, Y.
Urano, P. L. Choyke
10:00 —189. Design of novel non-viral gene vector based on head-tail type polycation
block copolymer. A. Harada, M. Kawamura, Y. Kimura, C. Kojima, K. Kono
10:20 —190. In vivo RNAi: New concepts and applications of siRNA-based therapeutic
agents. T. M. Rana
10:40 —191. Potent immunoconjugates for cancer therapy. P. D. Senter
Section C
Unknown Site -- Unknown Room
Commercialization of Biologics: Characterization and Improvement of Platforms to
Aid Commercialization of Biologics
S. Gadam and F. Olsen, Organizers
8:00 —192. A systems approach to characterization, validation and monitoring of
commercial processes. A. A. Shukla, S. Lee
8:20 —193. Systematic and logical strategies to reduce process scale-up and technology
transfer challenges from process development to manufacturing. T -S. Lee, M. McGill, P.
Thompson
8:40 —194. Evaluation of process and product impact of UVC treatment on a MAb
process. R. S. Rosenthal
9:00 —195. Viral clearance strategies using improved membrane adsorbers. W. T.
Riordan, M. R. Etzel
9:20 — Intermission.
9:40 —196. Standardization of purification process development for mAbs & Fc-fusion
proteins. R. Godavarti, G. Bolton, J. Booth, P. R. Brown, J. Coffman, C. Esenther, C.
Gallo, T. Iskra, S. Sun, M. Switzer, S. A. Tobler, R. Wright, B. Kelley
10:00 —197. Characterization of a reversed phase chromatography step. X. Hu, A. Wang,
Y. Lu, O. Kaltenbrunner
10:20 —198. Strategies for maximizing the clearance of impurities across an anion
exchange flow through step in the context of an integrated antibody purification process.
A. Kundu, A. Miller, J. Einerwold, M. Xia, R. Duffy, W. Zhou
10:40 —199. Platform ultrafiltration process development: Charge and conductivity
effects on ultrafiltration performance of monoclonal antibodies. A. S. Len, R. van Reis
Section D
Unknown Site -- Unknown Room
Upstream Processing: Advances in Cell Culture Process Development
S. T. Sharfstein, I. Blumentals, and G. Maheshwari, Organizers
8:00 — Introductory Remarks.
8:05 —200. High-producing CHO-GS and NS0-GS cell line generation and
characterization using Cell Xpress(TM) technology. N. Lin, G. Richardson, L. Davis, K.
Lacy, M. Gray, J. Cresswell, A. Dennett, M. Gerber, M. Caple, K. Kayser
8:25 —201. Developments in using the ACE System to generate high MAb expressing,
stable CHO cell lines. M. L. Kennard
8:45 —202. Proteomic and genomic studies of mammalian cell physiology to optimize
production of therapeutic and diagnostic proteins. D. Shen, T. R. Kiehl, S. T. Sharfstein
9:05 — Intermission.
9:25 —203. Impact of scale and bioreactor type on cell growth, productivity, product
quality, and global gene expression. M. A. Hanson, J. Vallejos, X. Ge, K. Brorson, A. R.
Moreira, G. Rao
9:45 —204. Sodium butyrate stimulates mAb over-expression in CHO cells by improving
gene accessibility. Z. Jiang, S. T. Sharfstein
10:05 —205. Improved analytical technique for high throughput analysis of glycoforms.
M. P. Gillmeister, N. Tomiya, S. J. Jacobia, Y. C. Lee, S. F. Gorfien, M. J. Betenbaugh
10:25 — Concluding Remarks.
Section E
Unknown Site -- Unknown Room
Elmer Gaden Award Lecture
W. Zhou, Organizer
11:30 —206. Directing the assembly of multifunctional biomolecular architectures. J. S.
Dordick
WEDNESDAY AFTERNOON
Section A
Unknown Site -- Unknown Room
BIOT Young Investigator Award Lecture
W. Zhou, Organizer
2:00 —207. Building proteins with new therapeutic functions from peptide modules. P. S.
Daugherty
Section B
Unknown Site -- Unknown Room
Emerging Technologies: Systems Biology
Cosponsored with BIOHW
C. Chan and C. Cho, Organizers
3:00 —208. Dissection of the HIV promoter and possible contribution of NFκB and Sp1
to phenotypic bifurcation and latency. J. C. Burnett, A. P. Arkin, D. V. Schaffer
3:20 —209. A new kinetic model reveals the synergistic effect of E-, P- and A-sites on
+1 ribosomal frameshifting. P -Y. Liao, P. Gupta, A. N. Petrov, J. D. Dinman, K. H. Lee
3:40 —210. Global assessment of protein turnover rates in Streptomyces coelicolor using
a multitagging proteomic strategy. K. P. Jayapal, S. Sui, R. J. Philp, Y -J. Kok, M. G.
Yap, T. J. Griffin, W -S. Hu
4:00 — Intermission.
4:20 —211. Universal Sequence Tag ARray (U-STAR) platform: A universal platform
for the quantitative analysis of transcriptomes. A. P. So, C. A. Haynes
4:40 —212. Proteome-wide identification of member-specific natural substrate repertoire
of caspases and other proteases. R. Liu, C. A. Valencia
5:00 —213. Cellular scale qualitative shotgun proteomic analysis of Nostoc sp. PCC
7120 isolated heterocysts and filaments under nitrogen deficient conditions using 8-plex
isobaric peptide tags. S. Y. Ow, K. Stensjö, P. C. Wright, P. Lindblad
Section C
Unknown Site -- Unknown Room
Biophysical and Biomolecular Symposium: Targeted Delivery of Proteins &
Nucleotides
C. Meares and W. Liu, Organizers
3:00 — Introductory Remarks.
3:05 —214. N-ethoxybenzylimidazoles (NEBIs) as potential pH-sensitive linkers for drug
delivery. A. Luong, S. D. Kong, G. Manorek, S. B. Howell, J. Yang
3:25 —215. Selective antitumor aptamer-photodynamic agent conjugates as a tool in
photochemo therapy. P. Mallikaratchy, W. Tan
3:45 —216. Synthesis, phototoxicity and metabolic stability of porphyrins conjugated to
bifunctional peptide sequences. M. G. H. Vicente, M. Sibrian-Vazquez
4:05 — Intermission.
4:20 —217. Optical antisense imaging of tumored mice using a fluorescent DNA duplex.
X. Liu, Y. Wang, K. Nakamura, G. Liu, S. Dou, A. Kubo, M. Rusckowski, D. J.
Hnatowich
4:40 —218. Specific cellular delivery of quantum dot-peptide and quantum dot-polymer
nanoassemblies. J. B. Delehanty, D. Farrell, I. L. Medintz, H. Mattoussi
5:00 —219. Transencapsidation of Semliki Forest virus RNA with tobacco mosaic virus
coat protein: Evaluation of antigen expression, antigen delivery and vaccine efficiacy. A.
A. McCormick, T. Corbo, J. Bernales, J. A. Lindbo, K. E. Palmer, M. L. Smith
Section D
Unknown Site -- Unknown Room
Commercialization of Biologics: Characterization and Improvement of Platforms to
Aid Commercialization of Biologics
S. Gadam and F. Olsen, Organizers
3:00 —220. Development and implementation of a robust chemically-defined and highyielding GS-CHO platform mAb production process. S. A. Casnocha
3:20 —221. Use of mathematical modeling to enable effective implementation of
platform aeration strategies in process scale-up and transfer. G. Nyberg, F. Jimenez, F.
Olsen, G. Grampp
3:40 —222. Robustness screening to enable cell culture process commercialization. A. M.
Rusiniak, T. Wang, G. Miroquesada
4:00 — Intermission.
4:20 —223. Process improvement through media development. W. Wenge
4:40 —224. Rapid development of mammalian cell production process of clinical
material for proof of principle of novel therapeutic molecules. J. Zhu, Y. Xie, V. V. Vyas,
B. Keseling, J. Roach, G. Mitra, S. P. Creekmore
5:00 —225. Rapid upstream commercial development of an Escherichia coli.-based
process for producing therapeutic proteins in inclusion bodies. H. Lin, I. Liu, H.
Barkhordarian, J. Lull, X. Yang
Section E
Unknown Site -- Unknown Room
Biophysical and Biomolecular Symposium: Protein Aggregation
C. J. Roberts and A. Grillo, Organizers
3:00 —226. Biophysical characterization of the amylin-derived peptide pramlintide. A.
Nonoyama, J. S. Laurence, L. Garriques, H. Qi, T. Le, C. R. Middaugh
3:20 —227. Silicone oil contamination of therapeutic protein formulations: Surfactant
and protein effects. J. Gabrielson, D. G. Bates, B. M. Williams, J -B. Hamel, J. F.
Carpenter, T. W. Randolph
3:40 —228. Self-interaction chromatography applied to the rapid development of
formulation for therapeutic proteins. H. Kornmann, N. Collet, G. Baer
4:00 — Intermission.
4:20 —229. Stability of a heterogeneous IgG1 antibody with atypical glycosylation: A
comparison between individual and mixtures of its glycoforms. G. C. Chu, S. B.
Coulibaly, H. K. Khor
4:40 —230. Discrepancies in the phase behaviors of therapeutic antibodies. B. Salinas, S.
Hasige, C. Allan, S. Bishop, A. Shah, J. F. Carpenter, T. W. Randolph
5:00 —231. Chemometric approach toward protein aggregation propensity. M. C.
Manning, C. M. Van Pelt, R. R. Meglen, F. J. Stevens
Symposium in Honor of Perry Frey
Sponsored by BIOL, Cosponsored with BIOT, MEDI, ORGN, BTEC, and BIOHW
WEDNESDAY EVENING
Section A
Unknown Site -- Unknown Room
Poster Session
Upstream Processing
Y. Tang, S. Ozturk, and S. Subramanian, Organizers
5:30 - 7:30
232. Role of astroglial fatty acid metabolism in the pathogenesis of Alzheimer's disease:
A metabolic flux analysis (MFA) study. S. Patil, C. Chan
233. Suppression of PKR activity mediates the apoptosis induced by palmitic acid and
TNF-α in HepG2 cells. X. Yang, C. Chan
234. Studies on novel optimization parameters for production of protease using response
surface methodology. A. I. R. Chaduvula, K. K. Nalla, M. Y. Nikku, S. B. Imandi, S. K.
Karanam, I. Yedluri
235. Evaluating biopharmaceutical economics and capacity with process modeling and
simulation. A. Koulouris, C. Siletti, D. Petrides
236. Monitoring the expression of biosynthetic pathway genes in Catharanthus roseus
hairy root cultures under optimum elicitation conditions. S. Goklany, R. H. Loring, C. W.
Lee-Parsons
237. Analysis of suspended and biofilm atrazine degrading cells. N. K. Biglione, V. G.
Rodgers, T. L. Peeples
238. Biosynthesis and application of colorants from fungi. F. Alihosseini, G. Sun
239. The evolution of a disposable stirred tank bioreactor at Centocor: From concept to
GMP implementation. W. Christian, R. Bhatia, N. Richardson, S. Ozturk
240. Biotransformation of steroids by Exophiala jeanselmei var. lecanii corni. K. P.
McCook, P. B. Reese
241. Multiplex transcript analysis for identifying high-producing cell lines. C. J. Lee, G.
Seth, J. Tsukuda, R. W. Hamilton
242. ReBiT, a database for enzymatic pathway design. C. H. Martin, D. Prather, K.
Jones Prather
243. Construction of ptb disrupted mutant for characterization of its function in
Clostridium tyrobutyricum fermentation. Y. Zhang, S -T. Yang
244. Biotransformation of steroids by Thielaviopsis paradoxa. F. A. Russell, P. B. Reese
245. Continuous perfusion process in a disposable stirred-tank bioreactor: A case study. B.
Chiang, N. Richardson, H. Haddock, C. Wood, R. Bhatia, S. Ozturk
246. Screening of strains for bioconversion from DL-ATC to L-cysteine. T. Dong, Z. Lin,
Y. Huang
247. Development of generic feed media for protein production in contract manufacturing.
G. Wang
248. Enhancement of mass transfer in a 250 L bioreactor by sparger design modification.
C. Wood, H. Haddock, B. Cottee, S. Ozturk
249. Fed-batch process development: A platform-based approach. K. Stoeber, C.
Ranucci, N. Villani
250. Fluid mixing practice for bioreactor scale-up and scale-down. R. D. LaRoche
251. Host system choice challenges bioprocessing of plant-derived therapeutics. L. R.
Wilken, S. Woodard, G. Barros, S. White, Z. L. Nikolov
252. Implementing an automated reactor sampling system for monitoring cell culture
bioreactors. L. Speciner, G. Barringer, J. Perez, C. Grimaldi, R. Reineke, A. Arroyo
253. Physiological characterization of Saccharopolyspora erythraea deletion strains:
Global and pathway specific regulators. I. A. Akinrinsola
254. Production of Galacto-oligosaccharides from whey lactose by using two-step PlugFlow Reactor with immobilized enzyme β-Galactosidase from Aspergillus oryzae and
Bacillus circulans and posterior chromatographic separation. J. I. Sanz Valero, S -T.
Yang
255. Rapid enhancement of antibody production through DOE-based hydrolysate
supplementation of an optimized base medium. C. Hunt, K. Hammett, T. Hill, T.
Oshunwusi, S. Holdread, Y. Bai, J. W. Brooks
256. Simultaneous determination of multiple components in CHO cell culture using
raman spectroscopy. S. Varma, S. Sakhamuri, M. Hausladen, Z. Li, S. S. Lee
257. Strategies for implementation of a new bioprocess container in commercial biologics
manufacturing. R. Marcklinger, C. Williamson, J. Gates, A. A. Shukla, S. Lee
258. Toward reproducible and optimal operation of mammalian cell cultures: A model
based approach for fault detection and isolation. S. Karra, M. N. Karim
259. Using CFD to model gas transfer in bioreactors. W. J. Kelly, J. Anderson
260. Improvement of the bleachability and brightness of wheat straw chemomechanical
pulp with a pretreatment of xylanase from Trichoderma reesei. F. Hong
261. Using MAb enhancing compounds to investigate the MAb production pathway. T. D.
Rau, R. R. Balcarcel
262. Analysis of gene dosage effects for metabolic engineering in Escherichia coli. N.
Negron Rodriguez, K. Schumacher, K. Jones Prather
263. Analysis of substrate metabolism for an immobilized CYP2C9 construct: A potential
bioreactor application. L. Wollenberg, J. L. Kabulski, T. S. Tracy, D. Lederman, P. M.
Gannett
264. Isolation and identification of acetic acid bacteria from Shanxi superior mature
vinegar in China. N. Yang, L. Hao
265. Effect of β-Mercaptoethanol on the fed-batch performance of GS-NS0 cells. Z. Naji,
S. Fries, M. Chartrain, C. Seamans
266. Engineering of an L-arabinose metabolic pathway in Corynebacterium glutamicum.
M. Sasaki, H. Kawaguchi, S. Okino, T. Jyojima, M. Inui, H. Yukawa
267. Novel large-scale production of genetically engineered elastin-like-biopolymer and
endotoxin-binding peptide fusion for neutralization of endotoxin. W. Jia, Y. Lei
268. Establishment of a WaveTM bioreactor scale-down model for process development
and characterization studies. S. F. Khattak, S. Sakhamuri, Z. Li, S. Lee
269. Expression of recombinant human erythropoietin having more complex N-glycans in
Drosophila S2 cells through hexosaminidase inhibition. Y. K. Kim, K. R. Kim, Y. H.
Kim, H. J. Cha
270. Inhibiting the apoptosis pathway using MDM2 in mammalian cell cultures. N. Arden,
B. S. Majors, S -H. Ahn, G. A. Oyler, M. J. Betenbaugh
271. Monitoring of glucose in cell culture by glucose binding protein (GBP) combined
with ultraslow microdialysis. X. Ge, M. A. Hanson, G. Rao, L. Tolosa
272. The impact of residual air in bioreactor sterilization. W. J. Kelly, S. Hannon, M.
Paddock, T. B. Vickroy
273. Optimization of monoclonal antibody production using process simulation and
scheduling tools. V. Papavasileiou, D. Petrides, C. Siletti
274. Proteomic investigation of elicited Eschscholzia californica cultures producing
enhanced levels of benzophenanthridine alkaloids. J. T. Oldham, M. Hincapie, T. Rejtar,
J. G. Dy, C. W. Lee-Parsons
275. Rational design of main-chain elongated substrates acceptable by the E.coli
ribosome. K. Mizusawa, S. Sando, Y. Aoyama
276. Using a global model of protein production with substrate competition to explain the
complex mRNA vs. protein relationship arising from genetic perturbations. R. S.
Kuczenski, K. H. Lee
277. Recombinant baculovirus-based multiple protein expression platform for Drosophila
S2 cell culture. K. R. Kim, Y. K. Kim, H. J. Cha
278. Systematic approach to troubleshooting in support of a late stage antibody
manufacturing campaign. L. Ly, M. Wyckoff, C. Lee, V. Tsang, H. Yusuf-Makagiansar
279. Reduction in high mannose glycoforms in mammalian cell culture through media
optimization. N. Le, B. Pendleton
280. Development of novel sourdoughs with the use of different cereal flours. H.
Hernandez-Sanchez, I. Nava-Arenas
281. Production of 14-oxo-cis-11-eicosenoic acid from lesquerolic acid by genetically
variable Sphingobacterium multivorum strains. T. M. Kuo, A. Rooney, T. A. Isbell
282. Production of D-lactic acid by the RITE bioprocess using genetically engineered
Corynebacterium glutamicum. S. Okino, M. Suda, M. Inui, H. Yukawa, Y. Tsuchida
283. Effects of redox buffers on the folding of disulfide containing proteins. W. J. Lees,
M. Gurbhele, F. Kibria
284. Bridging timescales between atomistic simulation and experiments with master
equation models of protein folding and dynamics. J. D. Chodera, N. Singhal, I. Haque,
W. C. Swope, J. W. Pitera, K. A. Dill, V. S. Pande
285. A simple and generic immunoplatform for detecting histidine tagged proteins based
on biofabrication. H -C. Wu, X. Shi, A. T. Lewandowski, C -Y. Tsao, R. Fernandes, C W. Hung, P. DeShong, E. Kobatake, G. F. Payne, W. E. Bentley
Section B
Unknown Site -- Unknown Room
Poster Session
Downstream Processing
Y. Tang, S. Ozturk, and S. Subramanian, Organizers
5:30 - 7:30
286. Simulation of an industrial ion exchange step: The use of simulation for robustness
analysis. T. B. Hansen, L. Sejergaard, E. Hansen, S. Kidal
287. Simulation of an industrial ion exchange step: The use of simulation to yield a
constant output with a variable input to a process step. S. Kidal, E. Hansen, L. Sejergaard,
T. B. Hansen
288. Strategies for integrating spectral data from raw materials to control
biopharmaceutical manufacture. M. Lanan
289. Characterization of peak broadening during size-exclusion chromatography of IgG2
antibodies. A. Leuras, E. Babcock, T. Li, R. Rajan
290. Cell culture conditioning to improve harvest performance and impurity removal. M.
Westoby, A. Koswara, R. Haverstock
291. Column simulations of chemically selective displacement systems. A. S. Freed, C. J.
Morrison, S. M. Cramer
292. Interrelating protein precipitation and hydrophobic interaction chromatography. B. K.
Nfor, K. Wiedhaup, T. Ahamed, L. A. M. van der Wielen, G. W. K. van Dedem, E. J. A.
X. van de Sandt, M. H. M. Eppink, M. Ottens
293. Purification of recombinant collagen from transgenic corn seed. C. Zhang, J. Baez,
C. E. Glatz
294. Activation of the IRF-3 transcription factor: The mechanisms of phosphorylationinduced IRF-3 dimerization and DNA binding. A. I. Dragan, V. Hargreaves, E. N.
Makeyeva, P. L. Privalov
295. Chromatographic HTS and simulation for improved process development and
optimization. M. Bensch, J. Nielsen, J. Krarup, T. B. Hansen, S. Kidal, L. Sejergaard
296. Impact of process parameters on the performance of Normal Flow Parvovirus (NFP)
filters in an antibody purification process. J. Einerwold, J. Jorgensen, B. Petersen, A.
Kundu
297. Stability of the bZIP homo- and heterodimers and energetics of their interactions
with DNA. R. Carrillo, A. I. Dragan, P. L. Privalov
298. Strategies for optimizing upstream clarification of cell culture fluids. B. Raghunath,
B. Cacace, D. Yavorsky
299. Accelerated physico-chemical characterization of complex protein mixtures for
optimized ion-exchange chromatographic separation. A. Berrill, S. Ho, N. TitchenerHooker, D. G. Bracewell
300. High throughput synthesis and screening for membrane filtration. M. Zhou, J. E.
Kilduff, D. G. Anderson, R. S. Langer, G. Belfort
301. Investigation of chemically selective displacers using robotic high throughput
screening, SPR, NMR and MD simulations. C. J. Morrison, S. A. McCallum, R.
Godawat, J. Moore, S. Garde, S. M. Cramer
302. Platform technology for developing purification processes. M. H. M. Eppink, R.
Schreurs II, A. Gijsen, B. Kamps, K. Verhoeven
303. Rapid optimization of Protein A chromatography using a high-throughput format. A.
Gronberg, F. Lindstrom, T. Bjorkman, G. Rodrigo
304. Streamline hydrophobic interaction chromatography resin screening for protein
purification process development. J. Ma, P. Jorjorian, C. Mason, R. Bates
305. Bench scale cleaning characterization of drug products in a fill-finish facility. N.
Rathore, N. Bhasin, P. Kolhe, R. Law, A. Abdul-Fattah, L. Li, W. Ji, K. Murphy
306. Regulatory strategy for changing a chromatography resin in a licensed process by the
use of a comparability protocol. M. J. Rubino, P. R. DeVoe
307. Chromatographic HTS and simulation for improved process development and
optimization. M. Bensch, J. Nielsen, J. Krarup, T. B. Hansen, S. Kidal, A. Stabe, J.
Hubbuch, E. Hansen, L. Sejergaard
308. Engineering the adsorption of plasmid DNA. W. J. Kelly, P. Butts
309. AKTA Crossflow for characterization of an ultrafiltration and diafiltration step. C.
W. Richey Jr.
310. Development of lab scale systems to evaluate micromixing in biological separations.
J. Girard, M. P. Watson
311. ELISA based selection of elution buffer for IgG-affinity chromatography. R.
Varakala, R. R. Beitle, K. Clark, T. W. Smith
312. Enantioselective hydrolysis of epoxides in organic solvents by Beauveria bassiana. E.
G. Ankudey, T. L. Peeples
313. Risk mitigation and optimization strategies for viral filtration. L. Flansburg, R.
Forng, S. Lee, A. A. Shukla
314. Robust microscale method for yeast cell disruption in the purification of intracellular
proteins. M. D. Wenger, P. DePhillips, D. G. Bracewell
315. Anomalous peak splitting of a human IgG2 mAb on HPLCSEC caused by charge
variants. J. Martin, M. Dupuis, J. Kwong, G. Waszak
316. Buffer distribution capacity analysis at the pilot scale. A. Caparoni, J. B. Fletcher, J.
Sinclair
317. Characterization of a new IgG binder adsorbent. P. Adielsson, S. Eriksson, F.
Alamravesh, H. Wlad, J. Färenmark
318. Characterization of cation exchange adsorbents for monoclonal antibody purification.
D. Roth, C. Daniels, B. To
319. Coimmobilization of pyranose 2-oxidase, laccase, and catalase on solid supports for
enhanced production of 2-keto-aldoses. R. Ludwig, P. Sukyai, T. Resic, D. Haltrich
320. Development of a high throughput purification method for analysis of cell culture
samples. S. Koob, N. Barron, J. H. Chou, M. Sinacore
321. Development of a high-capacity downstream process for an NS0-derived human
IgG2 based on 11-12 day fed batch fermentations with defined feed media. G. Waszak, E.
Yu, M. Dupuis, J. Martin
322. Development of Biacore application for influenza virus titer determination. C.
Estmer Nilsson, M. Bängtsson, A. Larsson, R. Lemmens
323. Monte-Carlo simulations of dye-labeled proteins diffusing in nanopores. J.
Hohlbein, M. Steinhart, E. Hinze, C. Schiene-Fischer, C. G. Hübner, U. Gösele
324. ELISA for Quantifying trace amount of an alkaline stabilized protein A in high
concentration of mAbs. T. Bjorkman
325. High resolution anion exchange chromatography purification of oligonucleotides. J.
K. O'Donnell, E. Gershenow, B. Kempf, S. Nakatani
326. Enhancing the thermostability of β-mannanase by addition of sugars, polylols and
their combinations. W. Wu, Z. Liu, W. Qi, Z. He
327. Evaluation of protein A chromatography media for capture of monoclonal antibodies.
B. R. Nielsen, J. S. Nielsen
328. Extraction of intracellular oxalate decarboxylase from the white-rot wood decay
fungus Trametes versicolor. C. X. Zhu, F. Hong
329. Impact of lot-to-lot variability of cation exchange resin on process performance. J.
Wahome, A. Berhe, J. Jorgensen, J. Einerwold, W. Zhou, A. Kundu
330. Impact of process parameters on the performance of Normal Flow Parvovirus (NFP)
filters in an antibody purification process. J. Einerwold, J. Jorgensen, B. Petersen, A.
Kundu
331. Isolation, identification, and characterization of heavy chain clips and an expressed
intron in a CHO-produced mAb. M. Dupuis, J. Finnessy, J. Leone, T. Carter, J. Martin
332. Kinetics and thermodynamics of thermal inactivation and the thermostable
mechanism of protective additives for beta-mannanase. Z. Liu, W. Wu, W. Qi, Z. He
333. Libraries of multimodal chromatographic ion exchanger for the discovery of new
selectivities and as a tool for HTPD. I. Blomqvist, P. Adielsson, J. Färenmark, J -L.
Maloisel
334. Mechanisms of protein A leaching. C. Victa, J. N. Franklin, P. J. McDonald, R.
Fahrner
335. Novel denitrification process of electrochemical biocatalyst and its stability. J. S.
Cho, J. Y. Park, Y. T. Park, Y. J. Yoo
336. Preparation and characterization of alcohol dehydrogenase-containing liposomes for
improving its quaternary structure. M. Yoshimoto, M. Sato, K. Nakao
337. Process-specific range finding of chromatography resin lots used in the purification
process of a recombinant product. L. Lee, J. Suchecki, K. Apgar, D. Abraham
338. Production of biodiesel by transesterification with new supported solid state alkali
catalyst CaO/NaY. H. Wu, Y. Zhou, J. Zhang, J. Liu
339. Successful scale-up of pH elution on cation-exchange column for the purification of
monoclonal antibodies. F. Zaman, J. Glynn, N. Ramasubramanyan, P. Mensah
340. Use of analytical ultracentrifugation with a fluorescence detector to characterize high
protein concentration formulations. Z. W. Kwong, J. Moore, J. Liu, Y. Shen, Q. Zhu, T.
Kamerzell, T. M. Laue, S. J. Shire
Section C
Unknown Site -- Unknown Room
Poster Session
Biophysical & Biomolecular Engineering
Y. Tang, S. Ozturk, and S. Subramanian, Organizers
5:30 - 7:30
341. A high-throughput genetic selection for protein folding and solubility in the bacterial
periplasm. T. Mansell, M. P. DeLisa
342. Creation of a fluorescent protein biosensor. E. Shusta, T. Pavoor
343. Designing AraC effector specificity through modeling and dual selection. P. C.
Cirino, S -Y. Tang, H. Fazelinia, C. D. Maranas
344. Gankyrin: A general model for Ankyrin Repeat (AR) protein studies. A. Mahajan,
Y. Guo, M -D. Tsai, J. Li
345. High yields of complex proteins with site-specific posttranslational modification
using cell-free protein synthesis. A. R. Goerke, J. J. Wuu, W. Ebina, J. R. Swartz
346. Mathematical model of protein secretion incorporating pore complex sequestration
by aggregated protein. R. S. Kuczenski, P. Gupta, K. H. Lee
347. New design strategies for FRET-based sensor proteins. M. Merkx, E. M. W. M. van
Dongen, T. H. Evers, J. L. Vinkenborg, L. W. J. Klomp, E. W. Meijer
350. Protein engineering of gene switches. H. Zhao, K. Chockalingam, M. McLachlan
351. Site-specific PEGylation of interferon–beta by Cu(I)–catalyzed cycloaddition. N. W.
Nairn, T. J. Graddis, A. Wang, K. Shanebeck, K. Grabstein
352. Stability of the bZIP homo- and heterodimers and energetics of their interactions
with DNA. A. I. Dragan
353. Structural stability and reactivity of bovine liver catalase modulated with liposomes.
M. Yoshimoto, H. Sakamoto, K. Nakao
354. Resolution of disulfide “scrambling” through engineering of a modified form of the
Nogo Receptor (NgR1) Fc fusion protein. P. H. Weinreb
355. Dendritic molecular transporters as highly targeted, controlled intracellullar delivery
vectors. B. L. Evans III, K. Huang, M. Ikizler, P. F. Wright, E. Harth
356. Directed evolution of targeted cell penetrating peptides for trans-BBB delivery. S.
Gao, M. Simon, B. Morrison III, S. Banta
357. Hemoglobin-drug conjugates for targeted delivery of nucleoside drugs. G. Adamson,
D. N. Bell
358. Ketalized polyethylenimine for tunable delivery of plasmid DNA and siRNA to
intracellular targets. M. S. Shim, Y. J. Kwon
359. Polyelectrolyte multilayer films as platforms for efficient siRNA delivery. S.
Kidambi, S. Mehrotra, J. A. Gredell, S. P. Walton, I. Lee, C. Chan
360. Targeting mRNAs with a radiolabeled herceptin/tat/antisense MORF streptavidin
nanoparticle. X. Liu, Y. Wang, K. Nakamura, A. Kubo, D. J. Hnatowich
361. The influence of different antitumor antibodies on anticancer effector accumulation
in solid tumors by MORF pretargeting. G. Liu, S. Dou, M. Rusckowski, D. J. Hnatowich
362. Two novel anti-TAG-72 cancer biomarkers identified by phage display. L. Chen, M.
Rusckowski, D. J. Hnatowich
363. Characterizing protein structure in amorphous solids using hydrogen/deuterium
exchange with mass spectrometry. Y. Li, T. D. Williams, R. L. Schowen, E. M. Topp
364. A highly efficient selection strategy for intracellular protein-protein interactions
mediated by a unique bacterial hitchhiker transport mechanism. D. Waraho, M. P.
DeLisa
365. Effects of water-miscible ionic liquids on the catalysis of horseradish peroxidase. K.
Ryu, E. Hong
366. Formulation and characterization of components in vaccines. J. D. Chesko, J.
Kazzaz, P. Malyala, D. O'Hagan, K. Patton, M. Singh
367. Amplification bias in competitive templates is offset through the use of proofreading
DNA polymerases: Application to the amplification of SAGE ditags. A. P. So, C. A.
Haynes
368. Dual fluorescence reporter system for studying +1 programmed ribosomal
frameshifting in Escherichia coli. P -Y. Liao, K. H. Lee
369. Stabilizing protein conformations in solution with osmolytes and cosolvents. C. H.
Schein
370. Highly active enzyme formulations for use in nonaqueous media. A. L.
Serdakowski, J. P. Lindsay, J. S. Dordick
371. Bioactive protein-based hydrogels for functional bioelectrode construction. I.
Wheeldon, S. Calabrese Barton, S. Banta
372. Determining antibody stability: Creation of defined interfacial effects within a high
shear environment. J. G. Biddlecombe, D. G. Bracewell, B. C. Fish, S. Uddin
373. Development and validation of a novel antioxidant capacity assay using sodA::gfp as
a living sensor. W. A. Lea, L. Tolosa, G. Rao
374. Improved solubility and stability of cytochrome c in hydrated ionic liquids. K. Fujita,
H. Ohno, D. R. MacFarlane, M. Forsyth
375. Surprising results of Pseudomonas aeruginosa heme oxygenase (pa-HO) ortho-19F
phenylalanine labeling. A. Wang, M. Rivera
376. Detection of biomolecules by magnetic reporting of labeled protein nanorods. T. P.
Harrah, J. Xie, J. I. Oh, M. J. Naughton, S. Sun, R. Guertin, E. B. Goldberg
377. Detection of s-phase cell cycle progression using ethynyl-2'-deoxyuridine
incorporation with click chemistry. S. B. Buck, S. T. Clarke, J. A. Bradford, K. Gee, B.
Agnew, A. Salic
378. Structure and dynamics of proteins from designed combinatorial libraries. A. Go, S.
Kim, J. Baum, M. H. Hecht
379. Quantifying the effects of electrostatic forces and microstructure of protein by the
change in Gibbs free energy of binding in IMAC. L. Pathange, D. Bevan, C. Zhang
380. Engineering of self-assembled vault protein nanocapsules. M. Yu, L. E. Goldsmith,
V. A. Kickhoefer, L. H. Rome, H. G. Monbouquette
381. Enzyme characterization at the nano-bio interface. J. L. Kabulski, L. Wollenberg,
M. Yang, N. N. Wu, T. S. Tracy, D. Lederman, P. M. Gannett
382. Fibrinogen self-assembled fibrils on Clay monolayer templates. J. Koo, T. Koga, D.
Galanakis, M. Rafailovich, J. Sokolov
383. Instantaneous distribution of cellular tractions during fibroblast migration. Z. Pan, Y.
Liu, K. Ghosh, T. Nakamura, R. A. F. Clark, M. Rafailovich
384. Integrated platforms for engineered cell-cell communication via cell-directed
assembly. E. C. Carnes, C. E. Ashley, D. M. Lopez, C. M. Douthit, D. R. Dunphy, C. J.
Brinker
385. Nanobiobased electrochemical biosensors for cisplatin detection. J. E. Jett, N. N.
Wu, H. Li, P. M. Gannett
386. Nanotube-assisted protein inactivation. A. Joshi, S. Punyani, T. Borca-Tasçiuc, R. S.
Kane
387. One pot synthesis and biotinylation of CdS nanocrystals by incorporating inteinmediated protein splicing. U. L. Lao, S. H. Kang, A. Mulchandani, W. Chen
388. Phytochelatin-mediated synthesis of cadmium chalcogenide semiconductor
nanocrystals. S. H. Kang, A. Mulchandani, W. Chen
389. Proteomic characterization of E. coli metal binding proteins. R. Varakala, R. Haley,
R. R. Beitle, M. M. Ataai
390. Screening the transcriptional machinery for potent artificial transcriptional activators.
C. Y. Majmudar, A. K. Mapp
391. Single molecule analysis of DNA/protein interactions: The nanopore shift assay. M.
Wanunu, D. Cohen-Karmi, Y. Yu, Z. Weng, A. Meller
392. Targeting gold probes to vault nanocapsule interiors using mINT “shuttles”. L. E.
Goldsmith, L. H. Rome, H. G. Monbouquette
393. The mechanism of arginine interaction with proteins. K. Tsumoto, D. Ejima, T.
Arakawa
394. Ultrafast sensing platform based on surface plasmon-coupled emission. D. S. Smith,
Y. Kostov, R. Badugu, G. Rao
395. A new strategy for acylation of tRNA with unnatural substrates. H. Masu, M.
Hayami, S. Sando, Y. Aoyama
396. Direct measurement of binding interactions among split inteins. M. Sorci, B. Dassa,
G. Anand, S. Pietrokovski, G. Belfort
397. Development of a novel method for direct nanopatterning of bacteriophage using
dip-pen nanolithography. S. F. Filocamo, C -Y. Chiang, A. M. Belcher, C. M. Mello
398. Facile and rapid direct gold surface immobilization with controlled orientation for
diverse carbohydrate types. J. H. Seo, D. G. Kang, Y. K. Kim, K. R. Kim, H. Y. Lee, H. J.
Cha
399. Impact of target mRNA structure on silencing efficiency and immune stimulation in
RNA interference. J. A. Gredell, A. Berger, S. P. Walton
400. Influence of α-gliadin and poly(hydroxyethyl methacrylate-co-4-styrenesulfonic
acid) concentrations on the structure of α-gliadin. L. Liang, J -C. Leroux, M. Subirade
401. Noncovalent immobilization of proteins on a solid surface by cucurbit[7]urilferrocenemethylammonium pair, a potential replacement of biotin-avidin pair. K. Kim, I.
Hwang, K. Baek, M. Jung, Y. Kim, K. M. Park, D -W. Lee, N. Selvapalam
402. Research of BODIPY fluorescence dyes-NHS derivates with high biochemical
activity. W. Dongchuan, P. Xiaojun, F. Jiangli
403. Sensing of nucleic acid sequence using unmodified DNA/RNA as a probe. A.
Narita, S. Sando, Y. Aoyama
404. Sterilizable optical sensor for alcohol measurements. Y. Kostov, G. Rao
405. Tyrosinase immobilization on carbon nanoparticles. K. S. Min, J. Y. Park, J. S. Cho,
Y. J. Yoo
Section D
Unknown Site -- Unknown Room
Poster Session
Stem Cells & Emerging Technologies
Y. Tang, S. Ozturk, and S. Subramanian, Organizers
5:30 - 7:30
406. Evaluation of UVC reactors using photochemically active fluorescent microspheres.
A. Lewis
407. Deregulation of CAP dependent translation can play a role in the malignant
transformation of eukaryotes. S. Nayak, J. K. Siddiqui, J. D. Varner
408. Identifying toxicity pathways through analysis of global transcriptional dynamics. Z.
Li, S. Srivastava, C. Chan
409. Systems analysis of cellular development and apoptosis in taxus cell lines. Y -J.
Yuan
410. Unsupervised classification and identification of physiologically relevant features by
integrating ontology information and expression data using Bayesian regression mixture
models. S. Srivastava, R. Jin, C. Chan
411. Coculture of human embryonic stem cells with murine embryonic fibroblasts on
microwell patterned substrates. A. Khademhosseini, L. Ferreira, J. Blumling, J. Yeh, J.
M. Karp, J. Junji Fukuda, R. Langer
412. An imaging chamber for cultures of human islets of Langerhans. D. Moogk, S.
Hanley, J. Ramunas, A. Blaylock, J. Skorepova, L. Rosenberg, E. Jervis
413. System to study colony-colony interactions in murine embryonic stem cells. S.
Sampattavanich, J. Voldman
414. Effects of fiber alignment on the behavior of human dermal fibroblasts on the 3-D
Poly (methyl methacrylate) (PMMA) electrospun scaffold. Y. Liu, Y. Ji, K. Ghosh, R. A.
Clark, J. C. Sokolov, M. H. Rafailovich
415. Hill colonies, a putative endothelial progenitor cell derived population, are nonclonal and result from aggregation of multiple cell types as determined using long term
imaging. C. S. Russell, A. Blaylock, J. Ramunas, L. Kelly, E. Clarke, C. G. Pereira, A. W.
Wognum, T. Thomas, A. Eaves, E. Jervis
416. Long-term expansion of embryonic stem cells in serum-free media containing plant
protein hydrolysates. N. Liu, S -T. Yang
417. Application of centrifugal force to enhance cell seeding in tissue engineering
scaffold. R. Ng, X. Zhang, S -T. Yang
418. Biomechanical properties of tissue engineered cartilage. F. Horkay, D. C. Lin, I.
Horkayne-Szakaly, E. K. Dimitriadis, C. Silva, P. J. Basser
419. Characterization and isolation of neurons differentiated from mouse ES cells by
DRG-conditioned medium. A. Kitazawa, N. Shimizu
420. Culture under low oxygen conditions markedly enhances differentiation of murine
embryonic stem cells into cardiomyocytes. J. R. Millman, D. E. Powers, J. P. M. Mattos
Almeida, C. K. Colton
421. Engineering polymerized hemoglobins for use in transfusion medicine. A. F.
Palmer
422. Enhanced albumin production by hepatic lineage cells derived from mouse
embryonic stem cells. C -C. Hsiao, C -H. Yin, W. Chen, C -L. Chen, W -T. Wu
423. Efficient ethanol production from glucose and xylose mixture using growth-arrested
Corynebacteria. S. Sakai, M. Sasaki, M. Suda, Y. Tsuchida, S. Okino, T. Jyojima, M.
Inui, H. Yukawa
424. Evaluation of production of bioethanol and biodiesel from renewable resources
using process simulation tools. D. Tsangaris, C. Siletti, D. Petrides
425. Heterologous expression of Hydrogenovibrio marinus NiFe-hydrogenase in
Escherichia coli for biohydrogen production. J. Kim, H. J. Cha
426. Lignin blockers to reduce costs of enzymatic hydrolysis of pretreated cellulose. C. E.
Wyman, B. Yang, D. Willies
427. Preparation of microbial oil by fermentation for biodiesel production. Y. Mo, H. Liu,
J. Zhang, X. Chen
428. Study on mild alkaline/oxidative pretreatment of bagasse. Z. Zhao, K. Cheng, J.
Zhang, F. Gao
THURSDAY MORNING
Section A
Unknown Site -- Unknown Room
Biophysical and Biomolecular Symposium: Protein Engineering
G. Makhatadze and R. S. Rajan, Organizers
8:00 —429. Engineering and design of repeat proteins. L. Regan, A. L. Cortajarena, G.
Haran
8:20 —430. Enzymatic activity from a designed artificial proteome. S. C. Patel, M. H.
Hecht
8:40 —431. Computational procedure for transferring new binding sites into existing
protein scaffolds. H. Fazelinia, P. C. Cirino, C. D. Maranas
9:00 —432. Dynamics in computational protein engineering. N. V. Dokholyan
9:20 — Intermission.
9:40 —433. Protein engineering of extremophilic chaperones for molecular self-assembly.
T. A. Whitehead, D. S. Clark
10:00 —434. Rational approaches to protein design and engineering. P. J. Reeder, C.
Bystroff, J. S. Dordick
10:20 —435. Structure-based humanization of therapeutic antibodies. K. Hanf, Y. Li, K.
Simon, L. L. Chen, M. Jarpe, E. Garber, F. Taylor, L. Silvian, J. Arndt, A. Lugovskoy
10:40 —436. Molecular strategy for designing robust proteins: Looking deeper at the
surface. G. Makhatadze
Section B
Unknown Site -- Unknown Room
Downstream Processing: Non-Chromatographic Separation Techniques: Improving
Process Throughput
G. Vedantham and D. W. Wood, Organizers
8:00 —437. Crystallization of therapeutic proteins and antibodies at Genentech:
Purification and bulk storage applications. B. A. Bean, T. C. Matthews
8:20 —438. Detergent-mediated liberation of intracellular recombinant virus-like
particles (VLPs) from S. cerevisiae homogenate. G. S. Kee, H. Pujar, N. TitchenerHooker
8:40 —439. Elastin-like polypeptide-intein mediated protein purification. A. R. Gillies,
D. W. Wood
9:00 —440. Large-scale antibody purification using non-chromatographic methods. S.
Ramanan, R. Stenson
9:20 — Intermission.
9:40 —441. Salt-dependent transmission of plasmid DNA through ultrafiltration
membranes. D. R. Latulippe, A. Zydney
10:00 —442. Selective precipitation using polyelectrolytes: A novel approach to the
purification of monoclonal antibodies. P. J. McDonald, J. Carter-Franklin, R. Fahrner
10:20 —443. Selective precipitation-assisted recovery of immunoglobulins from bovine
serum. A. Venkiteshwaran, P. Heider, G. Belfort
10:40 —444. Can affinity-sinking become an alternative to affinity-chromatography? G.
Patchornik
Section C
Unknown Site -- Unknown Room
Commercialization of Biologics: Scale-up & Process Design for Commercial
Facilities: Debottlenecking and Facility Fit
R. Kiss and N. Titchener-Hooker, Organizers
8:00 —445. Biopharmaceutical portfolio management and capacity planning: A
stochastic multiobjective optimization approach. E. D. George, S. Farid
8:20 —446. Gauging the technical, regulatory and financial implications of process
changes. I. Hassan, M. Bulmer, J. More, S. Farid
8:40 —447. Industrial case studies of debottlenecking and future proofing through
discrete event simulation. P. A. Sinclair
9:00 —448. Prototype software methodology for the rapid evaluation of
biomanufacturing process options. S. Chhatre, R. Francis, K. O'Donovan, N. J.
Titchener-Hooker, A. R. Newcombe, E. Keshavarz-Moore
9:20 — Intermission.
9:40 —449. Facility fit, when should it happen? J. D. Harms, A. M. Tsai, A. S. Rathore
10:00 —450. Facility fit: Ensuring process performance comparability for commercial
antibody processes when transferring to differently designed equipment. K. L. White, R.
Garcia, K. Carswell, E. Ordonez, A. Bhartiya, B. Jones, T. Renshaw, R. Kiss
10:20 —451. Strategies for increasing downstream capacity. D. H. Peers, M. Ratna
10:40 —452. Can modular construction help to address key bottlenecks in the retrofit
projects? T. Kimmel
Section D
Unknown Site -- Unknown Room
Upstream Processing: Advances in Tissue Engineering
J. B. Leach and A. Coury, Organizers
8:00 —453. Dual growth factor loaded PEGylated fibrin gels to control SMC phenotype
from human MSC. C. T. Drinnan, L. J. Suggs
8:20 —454. A 3-D tissue model for high-throughput drug discovery. X. Zhang, R. Ng, S
-T. Yang
8:40 —455. Intestinal epithelial cell response to crypt-like substrate topography. L.
Wang, S. K. Murthy, R. L. Carrier
9:00 —456. Novel cell adhesion biomaterial based on mussel adhesive protein fused with
RGD peptide. D. S. Hwang, Y. Gim, H. J. Cha
9:20 — Intermission.
9:40 —457. Tissue-like self assemblance of hepatocytes in vitro by manipulation of
collagen. Q. Meng, J. Dai, G. Zhang
10:00 —458. Anticancer drug test in tissue engineered perfusion microbioreactor array. Y.
Wen, X. Zhang, S -T. Yang
10:20 —459. Targeted oxygen delivery to hepatic hollow fiber bioreactors via
hemoglobin-based oxygen carriers. A. F. Palmer
10:40 —460. Novel systems for alginate-immobilized mammalian cell culture in
bioreactors. C. A. Hoesli, K. Raghuram, M. Luu, J. Osborne, G. Korbutt, J. M. Piret
THURSDAY AFTERNOON
Section A
Unknown Site -- Unknown Room
Biophysical and Biomolecular Symposium: Protein Engineering
G. Makhatadze and R. S. Rajan, Organizers
2:00 —461. Beyond biology: Engineering new structure and function with computational
protein design. J. G. Saven
2:20 —462. Directed evolution of conformational changes in peptides. M. Blenner, K.
Chockalingam, S. Banta
2:40 —463. Engineering allosteric inteins for use as biosensors or in bioseparations. A. R.
Gillies, D. W. Wood
3:00 —464. Insights into protein splicing and the engineering of inteins. B. Pereira, G.
Belfort, S. Garde, V. Derbyshire, M. Belfort
3:20 — Intermission.
3:40 —465. Picomolar affinity fibronectin antibody mimics by yeast display. B. Hackel,
K. D. Wittrup
4:00 —466. Intracellular ribosome display for selection of synthetic antibodies in the
bacterial cytoplasm. L. M. Contreras Martinez, M. P. DeLisa
4:20 —467. Structure-guided optimization of a therapeutic bispecific antibody. A.
Lugovskoy, K. Hanf, B. Miller, S. Demarest, J. Michaelson, X. Wang, E. Garber, J.
Browning, S. Glaser
4:40 —468. Engineering protein stability using polyethylene glycol conjugation: GCSF
as a case study. R. S. Rajan, T. Li, C. Sloey, M. Aras, W. Sutherland, D. N. Brems
Section B
Unknown Site -- Unknown Room
Downstream Processing: High Throughput Screening in Process Development
J. Coffman and G. Jagschies, Organizers
2:00 —469. Chromatographic HTS and simulation for improved process development
and optimization. A. Staby, M. Bensch, J. Nielsen, J. C. Krarup, T. B. Hansen, S. Kidal,
L. Sejergaard, E. Hansen, J. Hubbuch
2:20 —470. Development and application of an automated, small-volume
chromatography system for resin and condition screening. M. Teeters, D. Bezila, P.
Alred, A. Velayudhan
2:40 —471. Fast acquisition of bioseparation process development data from crude
protein mixtures. T. Ahamed, B. K. Nfor, E. J. A. X. van de Sandt, M. H. M. Eppink, P.
D. E. M. Verhaert, G. W. K. van Dedem, L. A. M. van der Wielen, M. Ottens
3:00 — Intermission.
3:20 —472. High throughput process development: Determination of dynamic binding
capacity using microtiter plates filled with chromatography resins. K. M. Lacki, T.
Bergander, A. Grönberg, K. Öberg
3:40 —473. High throughput screening of protein A resin for purification process
development. J. Booth, J. Coffman, B. Kelley
4:00 —474. High throughput screening in downstream processing – from solubility
studies to packed bed chromatography systems. J. Hubbuch
Section C
Unknown Site -- Unknown Room
Downstream Processing: Disposable Bioprocessing
D. D. Frey and S. Ramanan, Organizers
2:00 —475. Regulatory considerations for using disposable technologies in bioprocessing.
A. Moreira
2:20 —476. A comparison of conventional and controlled freezing methods. M. Olsen
2:40 —477. Disposable chromatography in antibody manufacturing: The good the bad
and the ugly. U. Gottschalk
3:00 — Intermission.
3:20 —478. Scalability of disposable Mixer system with a single-use levitating impeller.
A. Terentiev
3:40 —479. Use of a disposable tangential flow filtration skid to manufacture
monoclonal antibodies. H. Santhanam
4:00 —480. Viral clearance using disposable systems in Mab commercial downstream
process. J. Zhou
Section D
Unknown Site -- Unknown Room
Biophysical and Biomolecular Symposium: Protein Aggregation
T. Przybycien and M. Ricci, Organizers
2:00 — Introductory Remarks.
2:10 —481. Does one folding defect lead to another? The importance of quaternary
structure in transthyretin-mediated inhibition of beta-amyloid aggregation. L. Liu, J. Hou,
R. M. Murphy
2:35 —482. Folding, unfolding, and fibril formation of human eye lens γC-crystallin. Y.
Wang, J. King
3:00 —483. Molecular insights into self-perpetuating conformational changes of prions
and amyloids. P. M. Tessier, S. Lindquist
3:25 —484. Folding, misfolding, and aggregation of polyglutamine peptides and proteins.
M. Tobelmann, C. Lee, R. Walters, R. M. Murphy
3:50 — Intermission.
4:10 —485. Refolding aggregation-prone GPCRs and G-protein α subunits from
inclusion bodies expressed in Escherichia coli. E. C. McCusker, S. E. Bane, A. S.
Robinson
4:35 —486. Nonnative protein polymers: Structure, morphology, and relation to
nucleation and growth rates. W. F. Weiss IV, C. J. Roberts, A. M. Lenhoff
5:00 —487. Osmolyte controlled nucleation of insulin amyloid fibrillation. A. Nayak, C C. Lee, G. J. McRae, G. Belfort
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