Activity Template Subject Area(s) Life Science Associated Unit None Associated Lesson Activity Title None Natural Selection In Action: Tadpoles vs. Crayfish Header Image 1 ADA Description: ___? Caption: ___? Image file name: ___? Source/Rights: Copyright © ___? Grade Level _7 (6_-_8) Activity Dependency Time Required None 3 class periods of 52 minutes Group Size 28 Expendable Cost per Group US$20 This does not include the costs of laminating the cards since most schools have laminators available. Summary The purpose of this activity is to simulate evolution by natural selection. Students will participate in a game in which predators (crayfish) and prey (tadpoles) face off; tadpole tail size is the trait under selection in this simulation. Students will make predictions about what will happen after several rounds of predation and reproduction, play the game, reassess their predictions, and graph the distribution of tadpole tail sizes after predation. Engineering Connection Engineers use models to simulate natural phenomenon, as students will do in this exercise. Further, students practice graphing and interpreting data, crucial skills in engineering and science. Engineering Category Choose the category that best describes this activity’s amount/depth of engineering content: (1) relates science concept to engineering, (2) relates math concept to engineering, (3) provides engineering analysis or partial design, or (4) provides complete engineering design process. Or (5) does not include any engineering content. 1 or 5 Level of Inquiry Level 2: Structured Inquiry: students investigate a teacher-presented question through a prescribed procedure. Keywords adaptation, evolution, game, graph, natural selection Educational Standards 1) CA State Standards addressed: 7th Grade Science Genetics 7.2b. Students know sexual reproduction produces offspring that inherit half of their genes from each parent. 7.2c. Students know an inherited trait can be determined by one or more genes. Evolution 7.3a. Students know both genetic variation and environmental factors are causes of evolution and diversity of organisms. 7.3b. Students know the reasoning used by Charles Darwin in reaching his conclusion that natural selection is the mechanism of evolution. 7.3e. Students know that extinction of a species occurs when the environment changes and the adaptive characteristics of a species are insufficient for its survival. Investigation and Experimentation 7a. Select and use appropriate tools and technology (including calculators, computers, balance, spring scales, microscopes, and binoculars) to perform tests, collect data, and display data. 7c. Communicate the logical connection among hypotheses, science concepts, tests conducted, data collected, and conclusions drawn from the scientific evidence. 7d. Construct scale models, maps, and appropriately labeled diagrams to communicate scientific knowledge. 7e. Communicate the steps and results from an investigation in written reports and oral presentations. Math Statistics, Data Analysis, and Probability 1.0 Students collect, organize, and represent data sets that have one or more variables and identify relationships among variables within a data set by hand and through the use of an electronic spreadsheet software program: 1.1 Know various forms of display for data sets, including a stem-and-leaf plot or boxand-whisker plot; use the forms to display a single set of data or to compare two sets of data. 2) National Standards addressed: Science as Inquiry Standards Levels 5-8 Abilities necessary to do scientific inquiry Understanding about scientific inquiry Life Science Standards Levels 5-8 Populations and ecosystems Diversity and adaptations of organisms Science in Personal and Social Perspectives Levels 5-8 Populations, resources, and environments Pre-Requisite Knowledge Students should have learned about the concepts of evolution and natural selection already. They should also be familiar with how to calculate an average. Learning Objectives After this activity, students should be able to: Describe how natural selection works Relate the environmental conditions to whether or not a trait is adaptive Materials List To share with the entire class: Color print outs of tadpole cards, crayfish signs, and other signs indicating stations Laminating machine to laminate cards and signs 2 quarters Calculators for 2nd and 3rd days (or have students bring their own or borrow from math teacher) Clipboard for data recorder Paper for handouts (see attachments) Introduction / Motivation I had previously described my research to the students. In the Santa Monica Mountains, there are non-native crayfish in the streams that are eating the native tadpoles. I am researching whether the tadpoles are adapting to the crayfish and whether they are evolving any anti-predator traits to help them avoid being preyed upon by crayfish. Before beginning the game, I reminded the students about the tadpoles and crayfish in the Santa Monica Mountains and told them about how the introduced crayfish are eating the tadpoles. I started by asking the students about what traits they thought might be important for tadpoles to have to avoid being eaten by crayfish. I also asked students to define natural selection and we discussed that concept. Vocabulary / Definitions Word Definition Natural The process by which individuals that are better adapted selection to their environment survive and reproduce more successfully than less well adapted individuals; theory to explain the mechanism of evolution Adaptation A characteristic that improves an individual’s ability to survive and reproduce in a particular environment Evolution The process in which inherited characteristics within a population change over generations such that new species sometimes arise Trait Native species A genetically determined characteristic A species that is found in a particular region as the result of only natural forces, without human intervention Non-native invasive species Crayfish A species that is found in an area outside of its natural range due to human activity – frequently that species has negative impacts Crustacean that lives in fresh water – looks like a small lobster Procedure DAY 1 Tell the students that you went to a stream in the Santa Monica Mountains and collected tadpoles and you found that they had very different sizes of tails. You also noticed that there were crayfish in the streams (not native) that were eating the tadpoles. You then wondered if tail size might be important for determining whether a tadpole could escape predation by a crayfish. Ask the students how tail size might help or hurt a tadpole in escaping predation. Have them write down their hypothesis on “Hypothesis Handout” (Question1). Go over how the game will be played generally with an overhead schematic image – attached file “Game Schematic” Start by splitting up the class: 1 student will be the game leader 1 student will be the data recorder (will get a data sheet) 16 students to the tadpole group 2 students will be crayfish remaining students will be next generation of tadpoles Round 1: Give instructions to the game leader, have them follow instructions and tell other students what to do (help them as needed) 16 students that are tadpoles will select a card from the tadpole pile of cards – each student will be a tadpole with a specific sized tail that will be represented by an image and by a “tail score” Tail scores and the distribution of tail sizes are indicated in the tables below: Tail Score 2 (worst) 3 4 5 6 7 8 (best) Number 1 2 3 4 3 2 1 After tadpoles have received their tail sizes have them sit down and have the data recorder call out the tail scores and record the number of students with that tail score (can indicate by raising hands) Students that are crayfish will stand by the crayfish stations (denoted by a crayfish sign) Simulate Predation: Tadpoles will split up into approximately equal groups at each crayfish station When it is their turn, the crayfish student flips a quarter If the quarter shows heads: the tadpole does not encounter a crayfish and survives If the quarter shows tails: the tadpole encounters a crayfish The tadpole escapes predation and survives if their tail score is 6 or greater The tadpole is eaten is if their tail score is 5 or less Dead tadpoles go to “Dead Tadpoles” area and return their card to the ordered piles Surviving tadpoles go to “Survivor Tadpoles” area and get ready for reproduction Reproduction: After all tadpoles have gone through the predation cycle, the surviving tadpoles reproduce If you are a surviving tadpole: you reproduce and create 2 new tadpoles Since tail size is a heritable trait, offspring are similar but not identical to their parent (ask the students what is one simplification here? Answer: We are using only one parent in reproduction) One surviving tadpole produces two offspring, one with a tail size one level smaller than the parent, and one with a tail size one level higher than the parent (for instance, if the parent tail score is 6, they will produce 2 offspring, 1 with tail score 5, and one with tail score 7) You can get tail scores greater than 8 and less than 2, but not less than zero Have each surviving tadpole go through reproduction; the game leader should take the original card and hand out the new cards; the parent tadpole dies Two new students become offspring, if not enough students have dead tadpole or parent come back to life as an offspring or have students hold 2 cards This is the end of Round 1 Before beginning Round 2, the data need to be recorded Have all students sit down for the data to be recorded Have the data recorder write down the numbers of tadpoles with different tail scores as was done before Round 2: All tadpoles go through predation process again with the same rules as before All surviving tadpoles go through reproduction process as before Tally number at end of Round – record on data sheet by data recorder Repeat Rounds 3 times total At end, have students answer Question 2 from the “Hypothesis Handout” DAY 2 Pass out Data Analysis Handout Have students work in pairs – they should: 1. Copy down the raw data – on an overhead of “Data Recorder Sheet”, write in the data from that class that they collected the previous day and have students copy the numbers 2. Compute average tail scores for each round (will need calculators) – students will need help with this – do an example using an overhead of “Round 0 Example” 3. Walk students through this example – you need to start by multiplying the number of individuals by their tail size for each tail score class, add them up, and divide by the total number in that round 4. Graph tail size distribution of final tadpoles after Round 3 – students may need help with this – show how to draw in one sample on an overhead 5. Graph average tail size over time – encourage them to make a line graph 6. Describe how tail size changes with time 7. Reassess their hypothesis DAY 3 Have students finish the worksheet as many won’t finish on Day 2. Discuss follow up questions with students as a group. Possible answers are given but there may be other answers too. How did the trait of tail size change through the generations? ANS: tail size should increase over time because tadpoles with bigger tails are more likely to survive and reproduce Talk about other possibilities: Is this what is really happening in the streams? What are some simplifications? ANS: only one predator, there may be other traits more or equally important, crayfish is not evolving, etc… What if crayfish go away? ANS: there won’t be selection on tail size, will likely see a wider range of tail sizes after many generations, small tail size is no longer unfavorable – whether a trait is favorable or unfavorable depends on the environment What if another predator comes into the stream? ANS: if the other predator is very different from a crayfish, there may be selection on different tadpole traits, or even in the opposite direction as the selection by crayfish What if crayfish are such good predators that tadpoles almost never can escape predation? ANS: tadpoles would go extinct in those streams with crayfish Do you think there are other traits of the tadpole that are under selection? ANS: yes, possibly behavior (hiding), camouflage, size, speed, etc… Do you think there are traits of the crayfish under selection? ANS: yes, likely – ability to catch tadpoles, speed, vision, ability to smell tadpole chemical cues, claw size Is there any random process in this game? ANS: the flipping of the coin is the random process – organisms with a “bad” trait do not always get killed, they can survive and reproduce if they happen (randomly) to never encounter a crayfish – in this game, organisms with the “best” trait do always survive and reproduce which is an over simplification and not very realistic – in the real world, they may be subject to random events such as a drought or disease and die Background Before the Activity Before Day 1: Print out tadpole cards in color and laminate them – I printed 4 cards for tail scores 0-2 and 9-11 and 12 cards for tail scores 3-8 (however, I did run out a few times of specific cards so suggest printing more if possible – in a pinch, have a dry eraser marker and write the needed tail score on an unused card) Print out signs in color and laminate them – 2 crayfish signs, 1 of the others Make photocopies of handouts (Hypothesis and Data Analysis) and Data Sheets Make overhead of “Game Schematic” Before Day 2: Record data in from the game in a spreadsheet, make calculations Make overhead of data recorder sheet and fill in data for each class Make overhead of “Round 0 example” to show students how to calculate the average tail score for each round Make overheads of “Data Analysis” Handout to help students and show them example of how to graph if necessary With the Students 1. Image Insert Image # or Figure # here, [note position: left justified, centered or right justified] Figure 1 ADA Description: ___? Caption: Figure 1: ___? Image file name: ___? Source/Rights: Copyright © ___? Attachments Game Schematic Hypothesis Handout (Day 1) Data Analysis Handout (Day 2) Instructions for Game Leader Data Recorder Sheet Round 0 Example Tadpole Cards and Signs Safety Issues Troubleshooting Tips Day 1 can get a little chaotic and loud. Try to stay as organized as possible – having the students sit back down after the reproduction stage really helped regain a bit of order. The game works better for larger classes when you start out with 16 tadpoles in Round 0. By the end of Round 3 or even before, if you don’t have enough students, you will need to have students play the role of 2 or more tadpoles. If they do this, make sure to have them flip the coin once for each tadpole card they have. Having more tadpoles than students at the end of the game occurred in nearly all of the 9 classes in which this game was tested. If the class is very small, you could start with fewer than 16 tadpoles – for instance you could use 9 students to start with the tail distribution as below: Tail Score 2 (worst) 3 4 5 6 7 8 (best) Number 0 1 2 3 2 1 0 Investigating Questions See Day 3 questions. Assessment The questions on Day 3 will give you an idea as to whether the students got it. As well, grading the worksheets will help you to assess the students understanding of the concepts. I found that many students confused tadpole tail growth over generations for tadpole tail growth in individual in one lifetime. This might be something to focus on in discussion also. Pre-Activity Assessment Title: ___? Activity Embedded Assessment Title: ___? Post-Activity Assessment Title: ___? Activity Extensions Activity Scaling For lower grades, ___? For upper grades, ___? Additional Multimedia Support References Gendron, Robert P. “The Natural Selection Game”, Indiana University of Pennsylvania. Accessed November 5, 2008. http://nsm1.nsm.iup.edu/rgendron/LabsDownload.shtml Holt, Rinehart, and Winston. Life Science. Austin, TX: Harcourt Education Company, 2007. Photo/Image credits: Hypothesis handout and Data Analysis handout Tadpole image: http://www.pwrc.usgs.gov/TADPOLE/tadmorp.htm Crayfish image: http://www.freewebs.com/siganurme/seapea.htm Cards All photos by Katherine Pease Signs Reproduction: Photo by Gary Nafis from http://www.californiaherps.com/frogs/images/pregillaeggsbu308.jpg Dead Tadpoles: photo by Katherine Pease, photo by Mike Benard from http://www.mister-toad.com/PacificTreeFrog.html Survivor Tadpoles: photos by Katherine Pease Crayfish: http://www.malibuwatershed.org/2ndLevel/creaturefeature/creature_redsw ampcrayfish.html Schematic Tadpole: http://clean-water.uwex.edu/pubs/clipart/critters.tadpole.htm Crayfish: http://www.freewebs.com/siganurme/seapea.htm Other Redirect URL [For TE submissions only] Owner Katherine Pease, UCLA Science and Engineering of the Environment of Los Angeles, NSF GK-12 Contributors This activity has been classroom tested in the 7th grade classrooms at Emerson Middle School within the Los Angeles Unified School District under the guidance and help of teachers Elvia Park and Brian Gabrich. 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