SI Guide

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SI Guide
Supplementary Figure 1
MiR-122 is quantitatively recovered from a duplex formed with antagomir-122.
Equal amounts of miR-122 and antagomir-122 were annealed in vitro and added to a
Trizol/liver homogenate. RNA was extracted and samples were separated on a
polyacrylamide gel in the absence of formamide. The data demonstrate that miR-122 can
be quantitatively recovered from a miR-122/antagomir-122 duplex using the Trizol
protocol with ethanol precipitation.
Suppl.Fig. 1.pdf
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Supplementary Figure 2
Annealing antagomir-122 to liver RNA in vitro does not alter miR-122 detection in
formamide-containing gels.
Total liver RNA was incubated in the presence of different amounts of antagomir-122.
Samples were subjected to Trizol extraction and separated in polyacrylamide gels in the
presence or absence of 20% formamide. MiR-122 was detected by Northern blotting.
Suppl.Fig. 2.pdf
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Supplementary Figure 3
Sustained silencing of miR-122 after injection of antagomir-122 into mice.
a, Dose response relationship of total amount of antagomir-122 injected and silencing
activity in liver. b, Time-course study demonstrating that the silencing effect of
antagomir-122 is long-lasting.
Suppl.Fig. 3.pdf
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Supplementary Figure 4
Antagomirs specifically target miRNAs that derive from the same primary transcript.
Northern blots of total RNA isolated from liver and kidney after injection of antagomir192 or antagomir-194. The results demonstrate specific inhibition of miRNAs derived
from polycistronic precursors by antagomirs.
Suppl.Fig. 4.pdf
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Supplementary Table 1
Affymetrix gene expression analysis of genes up-regulated ≥1.4-fold in livers of mice
treated with antagomir-122 compared to control mm-antagomir-122.
Suppl.Table 1.xls
Microsoft Excel 124 KB
Supplementary Table 2
Affymetrix gene expression analysis of genes down-regulated ≥1.4-fold in livers of mice
treated with antagomir-122 compared to control mm-antagomir-122. Probe sets with
"absent (A)" to "absent" changes were excluded.
Suppl.Table 2.xls
Microsoft Excel 100 KB
Key genes
Hfe2
Tmed3
Slc35a4
AldoA
Ndrg3
Cs
Iqgap1
Hmgcr
Dhcr7
Acas2
Mvk
Hmgcs1
Fdps
Sqle
Fdft1
hemochromatosis type 2 (juvenile)
transmembrane emp24 protein transport domain containing 3
solute carrier family 35, member A4
aldolase A, fructose-bisphosphate
N-myc downstream regulated gene 3
citrate synthase
IQ motif containing GTPase activating protein 1
3-hydroxy-3-methylglutaryl-Coenzyme A reductase
7-dehydrocholesterol reductase
acetyl-Coenzyme A synthetase 2 (ADP forming)
mevalonate kinase
3-hydroxy-3-methylglutaryl-coenzyme A synthase 1
farnesyl diphosphate synthetase
squalene epoxidase
farnesyl-diphosphate farnesyltransferase 1
2
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