Curriculum Vitae
Date : 15 February 2016
Guangping Gao, Ph.D.
Home Address:
408 Yorkshire Road
Rosemont, PA 19010-1119
610-527-4680
Office Address:
2000, Translational Research Laboratories
125S, 31st Street
Philadelphia, PA 19104-3403
Tel: 215-898-2046
Cell: 484-686-7000 (Blackberry)
Fax: 215-898-6588
Citizenship:
U.S. Citizen
Education:
1978-82 B.M.
1988-90 M.S.
1990-94 Ph.D.
West China University of Medical Sciences
Florida International University
Florida International University (Biological Sciences)
Postgraduate Training and Fellowship Appointments:
1988-89
1994-96
Fellowship, Biomedical Engineering
World Health Organization/Florida International University, Miami
Postdoctoral Fellow, Gene Therapy, Institute for Human Gene Therapy
University of Pennsylvania, Philadelphia, PA
Faculty /Staff Appointment:
1986-1988
Instructor in Biochemistry, Department of Biochemistry, College of Pharmaceutical
Sciences, West China University of Medical Sciences, Chengdu, P.R. China
1996-1998
Assistant Director, Human Applications Laboratory, Institute for Human Gene Therapy
University of Pennsylvania, Philadelphia, PA
1998-2002
Associate Director, Institute for Human Gene Therapy
University of Pennsylvania, Philadelphia, PA
2003-present
Director, Vector Program, Gene Therapy Program, Department of Medicine
Division of Medical Genetics, School of Medicine
University of Pennsylvania, Philadelphia, PA
Other Appointments
2006-present
Deputy Director, Vaccine Research Institute
Joint Research Program of University of Pennsylvania and Sun Yat-Sen University
Guangzhou, China
Chair, Manufacturing Committee
Guangping Gao, M.D., Ph.D.
Page 2
International AAV Vector Reference Standard Working Group
Co-chairs for AAV vector sessions at annual meetings for
American Society of Gene Therapy
Abstract review and selection committees for ASGT Annual Meetings
Scientific review board of “Human Gene Therapy”
Ad hoc reviewer for
Current Gene Therapy
Gene Therapy
Journal of Virology
Molecular Therapy
Nature Medicine
Nature Biotechnology
And other gene therapy and virology journals
Invited for grant reviews by the following study sections:
NIH/NIAID, Integrated Clinical/Pre-Clinical HIV Therapeutics
NIH/NIDDK, Centers of Excellence in Hematology
Research Grants Council of Hong Kong, Infectious Diseases
Association Française contre les Myopathies
Membership in Honorary Societies
1989
Phi Kappa Phi
Memberships in Professional and Scientific Societies:
American Society of Human Genetics
American Society of Gene Therapy
American Association for the Advancement of Science
Lectures by Invitation and Chaired sessions at International conferences :
April 1, 1994
Baylor Medical College, Houston, TX
Sept 7, 1996
The 3rd Annual Retreat of the Institute for Human Gene Therapy, Absecon, NJ
June 3, 1997
Cystic Fibrosis Foundation Conference, Gene Therapy Session, Williamsburg, VA
Nov 8, 1998
The 5th Annual Retreat of the Institute for Human Gene Therapy, Absecon, NJ
June 9, 2002
American Society of Gene Therapy Annual Meeting, Boston, MA
August 28, 2002
IX Parvovirus Workshop, Bologna, Italy
June 5, 2003
American Society of Gene Therapy Annual Meeting, Washington, DC
June 6, 2003
American Society of Gene Therapy Annual Meeting, Washington, DC
March 4, 2004
University of North Carolina at Chapel Hill
June 2, 2004
American Society of Gene Therapy Annual Meeting, Minneapolis, ME
Guangping Gao, M.D., Ph.D.
Page 3
June 4, 2004
American Society of Gene Therapy Annual Meeting, Minneapolis, ME
Sept. 8, 2004
X Parvovirus Workshop, St. Petersburg, Florida
Sept. 30, 2004
NHF 7th Workshop on novel technologies and gene transfer for hemophilia B, Philadelphia, PA
Oct. 18, 2004
Symposium of Gene Therapy, Taipei, Taiwan
Dec. 3, 2004
University of Pittsburgh, Pittsburgh, PA
May 8, 2005
Gene Therapy Symposium, The Institute of Biomedical Sciences, Academia Sinica
Taipei, Taiwan
June 1, 2005
American Society of Gene Therapy Annual meeting, St. Louis, USA.
June 3, 2005
American Society of Gene Therapy Annual meeting, St. Louis, USA.
August 8, 2005
International conference for novel Biological Product Development, Mclean, VA, U.S.A.
March 31, 2006
NHF 8th Workshop on novel technologies and gene transfer for hemophilia B, Philadelphia, PA
May 30, 2006
American Society of Gene Therapy Annual meeting, Baltimore, MD
June 24, 2006
AAV gene vector manufacturing workshop, Organized by the Monegasque Association
against Muscular Dystrophy, Duchenne Parent Project France and Parent Project UK
Monte Carlo, Monaco
August 30, 2006
XI Parvovirus Workshop, Les Diablerets, Switzerland
June 3, 2007
American Society of Gene Therapy Annual Meeting, Seattle, WA
July 5th, 2007
Chinese Institute of Medical Sciences, Beijing, China
July 6th, 2007
Vector Gene Transfer Company, Beijing, China
Bibliography:
Chapters in Books and Reviews:
1.
Gao, G.P., Wivel N., and James M. Wilson. Production of Recombinant AAV, Viral Vectors for Gene Therapy. In Advances
in Virus Research. Edited by J.C. Glorioso. Academic Press, New York, 55:529-543, 2000
2.
Wivel N., Gao, G.P., and James M. Wilson. Adenoviral Vectors. The Development of Human Gene Therapy. Edited by
Theodore Friedman, Cold Spring Harbor Laboratory Press, New York, pp.87-110, 1999
3.
Gao, G.P., Vandenberghe, L.H. and Wilson J.M. New Serotypes of AAV Vectors. Current Gene Therapy, 2005, (3):285-97
4.
Gao, G.P., Vandenberghe, L.H. and Wilson J.M. Identification of Novel AAV Serotypes for Use as Vectors. GENE
THERAPY IN THE BRAIN, Edited by Michael G. Kaplitt and Matthew During, 2006.
Research Publications, Peer Reviewed:
1.
Susan L. Pichla_Gollon, Mark Drinker, Xiangyang Zhou, Feng Xue, John J. Rux, Guangping Gao, James M. Wilson,
Guangping Gao, M.D., Ph.D.
Page 4
Hildegund C.J. Ertl, Roger M. Burnett and Jeffrey M. Bergelson. Structure-Based Identification of a Major Neutralizing Site in
an Adenovirus Hexon. J. Virol. In press.
2.
Luk H. Vandenberghe, Lili Wang, Suryanarayam Somanathan, Yan Zhi, Joanita Figueredo, Roberto Calcedo, Julio
Sanmiguel, Ravi A. Desai, Christopher S. Chen, Julie Johnston, Rebecca L. Grant, Guangping Gao, and James M. Wilson.
Heparin binding directs activation of T cells against adeno-associated virus serotype 2 capsid. Nat Med, 12:967-971, 2006.
3.
Zhi Y, Figeuredo J, Kobinger G, Hagan H, Calcedo R, Miller J, Gao G and Wilson JM. Efficacy of a SARS vaccine based on
a non-human primate adenovirus in the presence of immunity against human adenovirus. Hum Gene Ther, 17:500-506,
2006
4.
Gao G, Yu L, Johnston J, Calcedo R, Grant R and Wilson JM. High level transgene expression in nonhuman primate liver
with novel AAV serotypes containing self-complementary genomes. J Virol, 80:6192-6194, 2006.
5.
Kelkar S, De B, Gao G, Wilson JM, Crystal RG and Leopold PL. A common mechanism for cytoplasmic dynein-dependent
microtubule binding shared among adeno-associated virus and adenovirus serotypes. J Virol, 80:7781-7785, 2006.
6.
Gao GP, You Lu, Roberto Caceldo, Grant Rebecca and James Wilson: Biology of novel AAV serotype vector mediated liver
gene transfer in NHPs. Molecular Therapy, 13:77-87, 2006.
7.
Kitajima K, Miller GC, Gao G, Marchadier DHL, Rader D and Wilson JM. Complete prevention of atherosclerosis in apoE
deficient mice by hepatic human apoE gene transfer with adeno-associated virus serotypes 7 and 8. Arterioscler Thromb
Vasc Biol, 26:1852-1857, 2006.
8.
Kobinger GP, Feldmann H, Zhi Y, Schumer G, Gao GP, Feldmann F, Jones S and Wilson JM. Simian Adenovirus Vaccines
Protects Against Zaire Ebola Virus, J Virol, 346:394-401, 2006.
9.
De BD, Heguy A, Hackett NR, Lee J, Pierre L, Gao G, Wilson JM and Crystal RG. High levels of persistent expression of
α1-antitrypsin mediated by the non-human primate serotype rh.10 adeno- associated virus despite pre-existing immunity to
common human adeno-associated viruses. Mol Ther, Mol Ther, 13:67-76, 2006
10. Tatsis N, Tesma L, Robinson E, Giles-Davis W, McCoy K, Gao G, Wilson JM and Ertl HCJ. Chimpanzee-origin adenovirus
vectors as vaccine carriers. Gene Ther, 13:421-429, 2006
11. Moscioni AD, Morizono H, McCarter RJ, Stern A, Cabrera-Luque J, Hoang A, Sanmiguel J, Wu D, Bell P, Gao G, Raper
SE, Batshaw ML and Wilson JM. Long-term correction of ammonia metabolism and prolonged survival in ornithine
transcarbamylase-deficient mice following liver-directed treatment with novel adeno-associated viral vectors. Mol Ther,
14:24-33, 2006.
12. Zhi Y, Kobinger GP, Jordan H, Suchma K, Shen H, Gao GP, Boyer JL, Crystal RG and Wilson JM. Identification of Murine
CD8 T Cell Epitopes in Codon Optimized SARS-Associated Coronavirus Spike Protein. Virology, 335:34-45, 2005.
13. Bell P, Limberis M, Gao GP, Wu D, Bove MS, Sanmiguel J and Wilson JM. An Optimized Protocol for Detection of E. coli
β- galactosidase in Lung Tissue following Gene Transfer. Histochem Cell Biol, 124:77-85, 2005.
14. Bell P, Wang L, Lebherz C, Flieder DB, Bove MS, Wu D, Gao GP, Wilson JM and Wivel NA. No Evidence for
Tumorigenesis of AAV Vectors in a Large Scale Study in Mice. Mol Ther, 12:299 306, 2005.
15. Lucy Deriy, Haruko Ogawa, Guangping Gao, and Uri Galili. In vivo targeting of vaccinating tumor cells to antigenpresenting cells by a gene therapy method with adenovirus containing the 1,3 galactosyltransferase gene. Cancer Gene
Therapy. 2005, 12:528-539.
16. Jennings K, Miyamae T, Traister R, Marinov A, Katakura S, Sowders D, Trapnell B, Wilson JM, Gao GP and Hirsch R.
Proteasome Inhibition Enhances AAV-Mediated Transgene Expression in cytes in Vitro and in Vivo. Mol Ther, 11:600-607,
2005.
Guangping Gao, M.D., Ph.D.
Page 5
17. Li Z, Qiao H, Lebherz, Choi SR, Zhou X, Gao G, Kung HF, Rader DJ, Wilson JM, Glickson JD and Zhou R. Creatine Kinase,
a Magnetic Resonance-Detectable Marker Gene for Quantification of Liver-Directed Gene Transfer. Hum Gene Ther,
16:1429-1438, 2005.
18. Rivera VM, Gao GP, Grant RL, Schnell MA, Zoltick PJ, Rozamus LW, Clackson T and Wilson JM. Long-Term
Pharmacologically Regulated Expression of Erythropoietin in Primates following AAV-Mediated Gene Transfer. Blood,
105:1424-30, 2005
19. Varnavski AN, Calcedo R, Bove M, Gao GP and Wilson JM. Evaluation of Toxicity from High-Dose Systemic Administration
of Recombinant Adenovirus Vector in Vector-Naïve and Pre-immunized Mice. Gene Thera, 12:427-436, 2005
20. Katakura S, Jennings K, Watanabe S, Adachi E, Thornton S, Gao GP, Wilson JM, Burstein H, Trapnell B and Hirsch R.
Recombinant Adeno-Associated Virus Preferentially Transduces Human, Compared to Mouse, Synovium: Implications for
Arthritis Therapy. Mod Rheumatol, 14: 18-24, 2004.
21. Hogan RJ, Gao GP, Rowe T, Bell P, Flieder D, Paragas J, Kobinger GP, Wivel NA, Crystal RG, Boyer J, Feldmann H, Voss
TG and Wilson JM. Resolution of Primary SARS-Associated Coronavirus Infection Requires Stat-1. J Virol,, 2004.
22. Rowe T, Gao GP, Hogan RJ, Crystal RG, Voss TG, Grant RL, Bell P, Kobinger GP, Wivel NA and Wilson JM. Macaque
Model for SARS. J Virol,, 2004.
23. Roy S, Gao GP, Clawson D, Farina SF and Wilson JM. Complete Nucleotide Sequences and Genome Organization of Four
Chimpanzee Adenoviruses. Virology, 324: 361-372, 2004.
24. Gao GP*, Roy S*., Lu Y, Zhou X, Lock M, Calcedo R and Wilson JM. Characterization of a Family of Chimpanzee
Adenoviruses and Development of Molecular Clones for Gene Transfer Vectors. Hum Gene Thera, 15: 519-530, 2004.
*Equal contribution.
25. Reyes-Sandoval A, Fitzgerald JC, Grant R, Roy S, Xiang ZQ, Li Y, Gao GP, Wilson JM and Ertl HCJ. HIV-1 Specific
Immune Responses in Primates upon Sequential Immunization with Adenoviral Vaccine Carriers of Human and Simian
Serotypes. J Virol, 78: 7392-7399, 2004.
26. Gao GP, Luk H. Vandenberghe, Mauricio R. Alvira, You Lu, Roberto Calcedo, Xiangyang Zhou and James M. Wilson.
Clades of Adeno-Associated Viruses are Widely Disseminated in Human Tissues. J Virol, 78: 6381-6388, 2004
27. Ziegler RJ, Lonning SM, Armentano D, Li C, Souza DW, Cherry M, Ford C, Barbon CM, Desnick RJ, Gao GP, Wilson JM,
Peluso R, Godwin S, Carter BJ, Gregory RJ, Wadsworth SC and Cheng SH. AAV2 Vector Harboring a Liver-Restricted
P
-galactosidase A and the Induction of Immune
Tolerance in Fabry Mice. Mol Thera, 9: 231-240, 2004
28. Gao GP, Lebherz C, Weiner DJ, Grant R, Calcedo R, Bagg A, Zhang Y and Wilson JM. Erythropoietin Gene Therapy
Leads to Autoimmune Anemia in Macaques. Blood, 103: 3300-3302, 2004.
29. Lebherz C, Gao GP, Louboutin JP, Millar J, Rader D and Wilson JM. Gene Therapy with Novel Adeno-Associated Virus
Vectors Substantially Diminishes Atherosclerosis in a Murine Model of Familial Hypercholesterolemia. J of Gene Med, 6:
663-672, 2004
30. Pinto AR, Fitzgerald J, Gao GP, Wilson JM and Ertl HCJ. Induction of CD8+ T Cells to an HIV-1 Antigen upon Oral
Immunization of Mice with a Simian E1-Deleted Adenoviral Vector. J Immunol, 171: 6774-6779, 2003
31. Xiang ZQ, Li Y, Gao GP, Wilson JM and Ertl HJC. Mucosally Delivered E1-Deleted Adenoviral Vaccine Carriers Induce
Transgene Product-Specific Antibody Responses in Neonatal Mice. J Immunol, 171:4287-4293, 2003.
32. Raper SE, Chirmule N, Lee FS, Bagg A, Gao GP, Heidenreich R, Wilson JM and Batshaw ML. Fatal Systemic Inflammatory
Response Syndrome in an Ornithine Transcarbamylase Deficient Patient following Adenoviral Gene Transfer. Mol Gen and
Metab, 80: 148-158, 2003.
Guangping Gao, M.D., Ph.D.
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33. Sarkar R, Tetreault R, Gao GP, Wang L, Bell P, Chandler R, Wilson JM and Kazazian HH. Total Correction of Hemophilia A
Mice with Canine FVIII Using an AAV8 Serotype. Blood, epub ahead of print, Oct 9, accession number 14551134, 2003.
34. Xiang ZQ, Gao GP, Reyes-Sandoval A, Li Y, Wilson JM and Ertl HC. Oral Vaccination of Mice with Adenoviral Vectors is not
Impaired by Preexisting Immunity to the Vaccine Carrier. J Virol, 77: 10780-10789, 2003.
35. Pinto AR, Fitzgerald JC, Giles-Davis W, Gao GP, Wilson JM and Ertl HCJ. Induction of CD8+ T Cells to an HIV-1 Antigen
through a Prime Boost Regimen with Heterologous E1-Deleted Adenoviral Vaccine Carriers. J Immunol, 171: 6774-6779,
2003.
36. Vinner L, Wee EGT, Patel S, Corbet S, Gao GP, Nielsen C, Wilson JM, Ertl HCJ, Hanke T and Fomsgaard A.
Immunogenicity in Mamu-A*01 Rhesus Macaques of a CCR5-Tropic Human Immunodeficiency Virus Type 1 Envelope from
the Primary Isolate (Bx08) after Synthetic DNA Prime and Recombinant Adenovirus 5 Boost. J Gen Virol, 84: 203-213,
2003.
37. Gao, G.P., Alvira, M.R., Somanathan, S., Lu, Y., Sanmiguel, J., Abbas, Z., Johnston, J., and Wilson, J.M. Adenoassociated viruses undergo substantial evolution in primates during natural infections. PNAS, 100:6081-6086, 2003.
38. Gao GP, Zhou X, Alvira MR, Tran P, Marsh J, Lynd K, Xiao W and Wilson JM. High Throughput Creation of Recombinant
Adenovirus Vectors by Direct Cloning, Green White Selection and I-Sce I Mediated Rescue of Circular Adenovirus Plasmids
in 293 Cells. Gene Therapy, 10: 1926-1930, 2003.
39. Xiang ZQ, Gao GP, Li Y, Wilson JM and Ertl HCJ. T Helper Cell- Independent Antibody Responses to the Transgene
Product of an E1-Deleted Adenoviral Vaccine Require NK1.1 T Cells. Virology, 305: 397-405, 2003
40. Fitzgerald J, Gao GP, Reyes-Sandoval A, Pavlakis GN, Xiang ZQ, Wlazlo AP, Giles-Davis W, Wilson JM and Ertl HCJ. A
Simian Replication-Defective Adenoviral Recombinant Vaccine to HIV-1 Gag. J Immunol, 170: 1416-1422, 2003
41. Johnston J, Tazelaar J, Rivera VM, Clackson T, Gao GP and Wilson JM. Regulated Expression of Erythropoietin from an
AAV Vector Safely Improves the Anemia of β-thalassemia in a Mouse Model. Mol Thera, in press.
42. Varnavski AN, Schlienger K, Bergelson JM, Gao GP and Wilson JM. Efficient Transduction of Human Monocyte-Derived
Dendritic Cells by Chimpanzee-Derived Adenoviral Vector. Hum Gene Thera, 14:533-544, 2003
43. Green AP, Huang JJ, Scott MO, Beaupre I, Meyers A, Gao GP and Wilson JM. A New Scaleable Method for the
Purification of Recombinant Adenovirus Vectors. Hum Gene Thera, 13:1921-1934, 2002.
44. Gao, G.P., Alvira, M., Wang, L., Calcedo, R., and Wilson, J.M. Novel adeno-associated viruses from rhesus monkeys as
vectors for human gene therapy. Proc Natl Acad Sci USA, 99: 11854-11859, 2002.
45. Auricchio, A., O’Connor, E., Weiner, D., Gao, G.P., Hildinger, M., Wang, L., and Wilson, J.M. Non-invasive gene transfer to
lung for systemic delivery of therapeutic proteins. J Clin Invest, in press, 2002.
46. Auricchio, A., Gao, G.P., Yu, Q.C., Raper, S., Rivera V., and Wilson, J.M. Constitutive and regulated expression of
processed insulin following In Vivo Hepatic Gene Transfer. Gene Thera, 9:962-970, 2002.
47. Derily L., Chen ZC, Gao GP and Galili U. Expression of -gal epitopes on HeLa cells transduced with adenovirus containing
1,3 galactosyltransferase cDNA. Glycobiology, 2002, 12:135-144.
48. Cohen, C., Xiang, Z.Q., Gao, G.P., Ertl, H.C.J., Wilson, J.M., and Bergelson, J.M. Chimapanzee adenovirus 68 adapted as
a gene delivery vector interacts with the coxsackievirus and adenovirus receptor (CAR). J Gen Virol, 83:151-155, 2002.
49. Gao, G.P., Lu, F.M., Sanmiguel, J.C., Tran, PT., Abbas, Z., Lynd, K.S., Marsh, J., Spinner, N.B., and Wilson, J.M. Rep/Cap
gene amplification and high yield production of AAV in an A549-Cell line expressing Rep/Cap. Mol Thera, 5:644-649,
2002.
Guangping Gao, M.D., Ph.D.
Page 7
50. Varnavski, A.N., Zhang, Y., Schnell, M., Tazelaar, J., Louboutin, J.P., Yu Q.C., Bagg, A., Gao, G.P., and Wilson, J.M.
Preexisting immunity to Adenovirus in rhesus monkeys fails to prevent vector-induced toxicity. J Virol, 76:5711-5719, 2002.
51. Xiang, Z.Q., Gao, G.P., Reyes-Synoval, A., Cohen, C.J., Li Y., Wilson, J.and Ertl, H.C.J. Novel, chimpanzee serotype 68based adenoviral vaccine carrier for induction of antibodies to a transgene product. J Virol, 76:2667-2675, 2002.
52. Auricchio, A., Hildinger, M., O’Connor, E., Gao, G.P. and Wilson J.M. Isolation of highly infectious and pure AAV2 vectors
with a single-step gravity-flow column. Hum Gene Thera, 12:71-76, 2001.
53. Cordier, L., Gao, G.P., Hack, A.A., McNally, E.M., Wilson, J.M., Chirmule, N., and Sweeney, H.L. Muscle-specific
promoters may be necessary for AAV-mediated gene transfer in the treatment of muscular dystrophies. Hum Gene Thera,
12: 205-215,2001.
54. Cordier, L, Gao, G.P., Wilson, J.M., Chirmule, N., and Sweeney, L.H. Use of muscle-specific promoter may be
necessaryfor AAV-mediated gene transfer in the treatment of muscle dystrophies. Hum Gene Thera, 12:205-215, 2001.
55. Dejneka, N.S., Auricchio, A., Maguire, A.M., Ye, X., Gao, G.P., Wilson, J.M. and Bennett, J. Pharmacologically-regulated
gene expression in the retina following transduction with viral vectors. Gene Thera, 8:442-446, 2001
56. Eck, S.L., Alavi, J.B., Judy, K., Phillips, P., Alavi, A., Hackney, D., Cross, P., Hughes, J., Gao, G.P., Wilson, J.M., and
Propert, K. Treatment of recurrent or progressive malignant glioma with a recombinant adenovirus expressing human
interferon-beta (H5.010CMVhIFN- ): A phase I trial. Hum Gene Thera, 12:97-113, 2001.
57. *Gao, G.P., *Farina, S.F., Xiang, Z.Q., Rux, J.J., Burnett, R.M., Alvira, M.R., Marsh, J., Ertl, H.C.J., and Wilson, J.M. A
replication defective vector based on a chimpanzee adenovirus. J Virol, 75:11606-11613, 2001. . *Equal contribution.
58. *Gao, G.P., *Tao, N., *Parr, M., Johnston, J., Baradet T., Wilson, J.M., Barsoum, J., and Fawell, S.E. Sequestration of
adenoviral vector by Kupffer cells lads to a non-linear dose response of transduction in liver. Mol Therapy, 3:28-35,
2001.*Equal contribution
59. Hildinger, M., Auricchio, A., Gao, G.P., Wang, L., Chirmule, N.and Wilson, J.M. Hybrid vectors based on adeno- associated
virus serotypes 2 and 5 for muscle-directed gene transfer. J Virol, 75:6199-6203, 2001.
60. Odaka, M., Sterman, D.H., Wiewordt, R., Zhang, Y., Kiefer, Amin K., Gao, G.P., Wilson, J.M., Barsoum J., Kaiser, L. and
Albelda, S.M. Eradication of intraperitoneal and distant tumor by adenovirus mediated interferon beta gene transfer due to
induction of systematic immunity. Cancer Research, 61:6201-6212, 2001
61. Raper, S.E., Yukoff, M., Chirmule, N., Gao., G.P., Nunes, F., Haskal, Z.J., Furth, E.E., Propert, K.J., Robinson, M.B.,
Magosin, S., Simoes, H., Speicher, L., Hughes, J., Tazelaar, J., Wivel, N.A., Wilson, J.M. and Batshaw, M.L. A pilot study of
in vivo liver-directed gene transfer with an adenovirus vector in partial ornithine transcarbamylase deficiency. Hum Gene
Thera, 13:163-175, 2001.
62. Schnell, M.A., Zhang, Y., Tazelaar, J., Gao, G.P., Yu, Q.C., Qian, R., Chen, S., Varnavski, A.N., LeClair, C., Raper, S.E.,
and Wilson, J.M. Activation of innate immunity in nonhuman primates following intraportal administration of adenoviral
vectors. Mol Therapy, 3:708-722, 2001.
63. Zhang, Y., Schnell, M.A., Tazelaar, J., Gao., G.P., Yu, Q.C., Qian, R., Chen, S., Varnavski, A., Le Clair C., Raper S.E., and
Wilson, J.M. Activation of innate immunity in nonhuman primates following intraportal administration of adenovirus vectors.
Mol Therapy, (in press), 2001.
64. Zhang, Y., Chirmule, N., Gao., G.P., Qian, R., Croyle, MA., Joshi, B.,Tazelaar, J., and Wilson, J.M. Acute cytokine
response to systematic adenoviral vectors in mice is mediated by dendritic cells and macrophages. Mol Therapy, 3:697-707,
2001.
65. Zoltick, P.W., Chirmule N., Schnell M.A., Gao, G.P., Hughes, J.V. and Wilson, J.M. Biology of E1-deleted adenovirus
vectors in nonhuman primate muscle. J Virol, 75:5222-5229, 2001.
Guangping Gao, M.D., Ph.D.
Page 8
66. Cordier, L., Hack, A.A., Scott, M.O., Barton-Davis, E.R., Gao, G.P., Wilson, J.M., McNally, E.M., and Sweeney, H.L.
Rescue of skeletal muscles of Gamma-sarcoglycan deficient mice with AAV-mediated gene transfer. Mol Therapy, 1: 119129. 2000
67. Chen, J.C., Rader, D.J., Tazelaar, J., Kawashiri, M., Gao, G.P., and Wilson, J.M. Prolonged correction of hyperlipidemia in
mice with familial hypercholesterolemia using an adeno-associated viral vector expressing VLDL receptor. Mol Therapy,
2:256-261, 2000
68. Gao, G.P., Qu, G., Burnham, M.S., Huang, J., Chirmule N., Joshi., B., Yu, Q.C., Marsh, J .M, Conceicao C. A. and Wilson,
J.M. Purification of recombinant adeno-associated virus vectors by column chromatography and its performance in vivo.
Hum Gene Thera, 11:2079-2091, 2000.
69. Gao, G.P., Engdahl, R.K. and Wilson, J.M. A cell line for high-yield production of E1-deleted adenovirus vectors without the
emergence of replication-competent virus. Hum Gene Thera, 11:213-219, 2000.
70. Sarkar, R., Gao, G.P., Chirmule R., Tazzelaar, J., and Kazazian, H. Jr. Partial correction of murine hemophilia A with Neoantigenic murine factor V III. Hum Gene Thera , 11:881-894, 2000.
71. Watanabe, S., Imagawa, T., Boivin, G.P., Gao, G.P., Wilson, J.M., And Hirsch, R. Adeno-associated virus mediates longterm gene transfer and delivery of chondroprotective IL-4 to murine synovium. Mol Therapy. 2:147-152,2000
72. Zhang, Y., Chirmule, N., Gao, G.P., and Wilson, J.M. CD40 ligand dependent activation of cytotoxic T lymphocytes by AAV
vectors in vivo: Role of immature dendratic cells. J Virol, 74:8003-8010, 2000
73. Bennett J., Maguire A.M., Cideciyan A.V., Schnell M., Glover E., Anand V., Aleman T.S., Chirmule N., Gupta A.R., Huang
Y., Gao G.P., Nyberg W.C., Tazelaar J., Hughes J., Wilson J.M., and Jacobson S.G. Stable transgene expression in rod
photoreceptors after recombinant adeno-associated virus-mediated gene transfer to monkey retina. Proc Natl Acad Sci
USA, 96:9920-9925, 1999.
74. Chirmule, N., Truneh, A., Haecker, S., Tazelaar, J., Gao, G.P., Raper, S., Hughes, J.V., Wilson, J.M. Repeated
administration of adenoviral vectors in lungs of human CD4 transgenic mice treated with a non-depleting CD4 antibody. J.
Immunol, 163:448-455, 1999.
75. Greelish JP., Su L.T., Lankford E.B., Burkman J.M., Chen H., Konig S.K., Mercier I.M., Desjardins P.R., Mitchell M.A.,
Zheng X.G., Leverovich J., Gao G.P., Balice-Gordon R.J., Wilson J.M. and Stedman H. Stable restoration of the
sarcoglycan complex in dystrophic muscle perfused with histamine and a recombinant adeno-associated viral vector. Nat
Med, 5:439-443, 1999.
76. Grifman M, Chen N.N., Gao, G.P., Cathomen T., Wilson J.M. and Weitzman M. Overexpression of cyclin A inhibits
augmentation of recombinant adeno-associated virus transduction by the adenovirus E4orf6 protein. J Virol, 73: 1001010019, 1999.
77. Lanuti, M., Gao, G.P., Force, S.D., Chang, M.Y., E1 Kouri, C., Amin, K., Hughes, J.V., Wilson, J.M., Kaiser, L.R., Albelda,
S.M. Evaluation of an E1/E4-deleted adenovirus expressing the herpes simplex thymidine kinase suicide gene in cancer
gene therapy. Hum Gene Thera, 10:463-475, 1999.
78. Xiao, W.D., Chirmule, N., Berta, S.C., McCullough, B., Gao, G.P., Wilson, J.M. Gene Therapy Vectors Based on Adenoassociated Virus Type I. J Virol, 73:3994-4003, 1999.
79. Ye X.H., Rivera, V.M., Zoltick, P., Cerasoli F., Schnell, M.A., Gao, G.P., Hughes, J.V., Gilman, M., Wilson, J.M. Regulated
delivery of therapeutic proteins after in vivo somatic cell gene transfer. Science, 283:88-91, 1999.
80. Chirmule, N., Hughes, J.V., Gao, G.P., Raper, E.S., and Wilson, J.M. The role of E4 in eliciting CD4 T-cell and B-cell
responses to adenoviral vectors delivered to murine and nonhuman primate lungs. J Virol, 72:6138-6145, 1998.
Guangping Gao, M.D., Ph.D.
Page 9
81. Gao, G.P., Qu, G., Faust, Lynn Z., Engdahl, Ryan K., Xiao, W.D., Hughes, J.V., Zoltick, P.W., and Wilson, J.M. High-titer
adeno-associated viral vectors from a rep/cap cell line and hybrid shuttle virus. Hum Gene Thera, 9:2353-2362, 1998.
82. Raper, S.E., Haskal, Z.J., Ye, X.H., Pugh, C., Furth, E.E., Gao, G.P., and Wilson, J.M. Selective gene transfer into the liver
of non-human primates with E1-deleted, E2A- defective or E1-E4 deleted recombinant adenoviruses: a preclinical toxicology
study. Hum Gene Thera, 9:671-679, 1998.
83. Ye X., Gao G-P, Pabin C, Raper S.E. and Wilson J.M. Evaluating the potential of germ line transmission after intravenous
administration of recombinant adenovirus in the C3H mouse. Hum Gene Thera, 9:2135-2142, 1998.
84. Fisher, K.J., Gao, G.P., Weitzman, M.D., Dematteo, R., Burda,J.F., and Wilson, J.M. Trandsduction with recombinant
adeno-associated virus for gene therapy is limited by leading-strand synthesis. J Virol, 70:520-532, 1996.
85. Gao, G.P., Yang, Y.P., and Wilson J.M. Biology of adenoviral vectors deleted of E1 and E4 for liver-directed gene therapy.
J. Virol, 70:8934-8943, 1996.
86. Gao, G.P. and Herrera R. Enrichment of middle repetitive element Bm-1 transcripts in
fractions of the silkmoth, Bombyx mori. Genetica, 97:173-182, 1996.
translationally active RNA
87. Kaul, R*., Gao, G.P., Matalon, R., Aloya, M., Su, Q., Jin, M., Johnson, A.B., Schutgens, R.B.H., and Clarke, J.T.R.
Identification and expression of eight novel mutations among non-Jewish patients with Canavan disease. Am J Hum Genet
59:95-102, 1996.
88. Kaul, R*., Gao, G.P., Michals, K., Whelan, D., Levin, S.,and Matalon, R. (1995) Novel cys152 -> arg missense mutation in
Arab patients with Canavan disease. Hum Mutat, 5:269-271, 1995.
89. Matalon, R., Kaul, R*., Gao, G.P., Michals, K., Gray, R.G.F., Bennett-Briton, S., Norman, A., Smith, M., and Jakobs,
Prenatal diagnosis for Canavan disease: the use of DNA markers. J. Inhert Metabol Dis, 16:215-217, 1995.
90. Kaul, R*., Balamurugan, K., Gao, G.P., and Matalon, R. Canavan disease: genomic organization of human ASPA to 17p13ter; conservation of the ASP gene during evolution. Genomics, 2:364-370, 1994.
91. Kaul, R*., Gao, G.P., Balamurugan, K., and Matalon, R. Canavan Disease: molecular basis of spartoacylase deficiency. J
Inhert Metabol Dis,. 17:295-297, 1994.
92. Kaul, R*., Gao, G.P., Aloya, M., Balamurugan, K., Petrosky, A., Michals, K., and Matalon, R*. Canavan Disease: mutations
among Jewish and Non-Jewish Patients. Am J Hum Genet, 55:34-4, 1994.
93. Kaul, R*., Gao, G.P., Balamurugan, K., and Matalon, R. Cloning of the human aspartoacylase cDNA and a common
missense mutation in Canavan Disease. Nature Genet, 5:118-123, 1993.
*Ph.D. dissertation advisor and the publications generated from my dissertation project
94. von Sternberg, R.M., Novick, G.E., Gao, G.P., and Herrera, R.J. Genome canalization: the coevolution of transposable and
Interspersed repetitive elements with single copy DNA Genetica, 86:215-246, 1992.
95. Yang, J.Y., Gao, G.P., Peng, X.F., Zeng, R., and Zhu, X.F. Studies on the new technique of preparing pancreatin from
swine pancreas in accordance with the standard of the three enzymes in the Chinese Pharmacopeia, (1986). West China J.
Pharmaceutical Sci., 3:15-18, 1988.
96. Yang, JY., Gao, G.P., Peng, X.F., Zeng, R., and Zhu, X.F. The relation among the activities of three enzymes in the
process of preparing pancreatin from swine pancreas. West China J. Pharmaceutical Sci, 2:198-202, 1988.
Patents:
1.
Gao.G.P, Wilson,J.M, and Ertl. Method for rapid screening of bacterial transformants and novel simian adenovirus proteins.
Guangping Gao, M.D., Ph.D.
Page 10
2.
Gao, G.P., Wilson, J.M. and Zhou, X. Y. Polyvalent viral vectors and system for production thereof.
3.
Vandenberghe, L., Gao, G.P., and Wilson, J.M. Method of increasing the function of an AAV vector.
4.
Wilson,J.M, Gao.G.P, Vandenberghe, L.H and Mauricio, R. A. AAV Clades, Sequences, vectors contained same, and uses
Thereof
5.
Gao, G.P and Wilson J.M. Method for direct rescue and amplification of integrated viruses from cellular DNA of tissues.
6.
Gao, G.P. Wilson, J.M, and Alvira MR. Method of detecting and/or identifying AAV Sequences and isolating novel
sequences identified thereby.
7.
Gao, G.P. Wilson, J.M, and Alvira MR. Novel AAV Serotype 8 Sequences, and vectors containing same, and uses
therefore
8.
Gao, G.P. Wilson, J.M, and Alvira MR. Novel AAV Serotype 9 Sequences and uses therefore
9.
Gao, G.P. and Wilson, J.M. Methods for efficient rescue and high throughput creation of adenovirus vectors.
10. Wilson, J.M., Gao, G.P. , and Roy, S. Chimpanzee adenovirus nucleic acid and amino acid sequences, vector construction
containing same, and methods of use.
11. Gao, G.P. , Wilson, J.M and Alvira, M.R., Compositions and methods for production of recombinant viruses and uses
therefore.
12. Zoltick.P, Gao, G.P. and Wilson.J.M. Regulatable gene expression system.
13. Gao, G.P. and Wilson, J.M. Compositions and methods for increasing packaging and yields of recombinant
adenoviruses using multiple packaging signals.
14. Gao G.P. and Wilson, J.M. Cell lines and constructs useful in production of E1-Deleted adenovirus in absence of replication
competent adenovirus.
15. Gao, G.P. and Wilson, J.M. Compositions ands Methods for helper-free production of recombinant adeno-associate
viruses.
16. Gao, G.P. and Wilson, J.M., and Methods and cells lines for production of recombinant adeno-associated viruses.
17. Gao, G.P. and Wilson, J.M., and Novel adenovirus gene therapy Vehicle and cell lines.
18. Wilson, J.M., Fisher, K., and Gao, G.P. Recombinant adenovirus and adeno-associated virus.
19. Wilson, J.M., and Gao, G.P. Method for improved production of recombinant adenovirus vectors.
20. Matalon, R., Kaul, R., Gao, G.P., Balamurugan, K., Michals-Matalon, K. Aspartoacylase gene, protein, and methods of
screening formutations associated with Canavan disease.
Guangping Gao, M.D., Ph.D.
Page 11
Past Grant Support
NAME
Guangping Gao
DEPARTMENT
Pathology and Laboratory Medicine
Grant Name & Sponsor
Number
Period of
Award
Grant
Category*
Role in Grant
**
Juvenile Diabetes
Foundation International
070199 063005
PG
AAV Collaboration with
ARIAD
041502011403
Gene Therapy for
Malignant Mesothelioma
Improved Adenoassociated Virus
Purification
P30DK19525
Diabetes Endocrinology
Research Center
Creation of Vector
Systems for Gene
Therapy
P30DK47757
Gene Therapy for Cystic
Fibrosis and other
Genetic Disease
DATE
2/15/2016
% Effort
Funding
Source
Annual Direct
Cost
Additional
Comments
Project
Leader
15%
JDRF
$164, 671
($1,388,892)
PI: Wilson
IG
Project
Leader
25%
ARIAD
$293,782
PI: Wilson
051800033105
PP
Project
Leader
5%
NIH/NCI
P01
($1,252,292)
PI: Albelda
031502022804
IG
PI
5%
Puresyn Inc
$190,162
040100033103
PP
Project
Leader
5%
NIH/NIDDK
$44,000
070100063005
IG
Project
Leader
10%
GSK
$235,980
(2,100,000)
PI: Wilson
19982003
PP
Project
Leader
NIH/NIDDK
$68, 369
PI:Wilson
**For Grant Category, use code in bold from the following menu :
R01 NIH R01
PP NIH Program Project, Center or Core Grant
FG Federal Grants – Other (including other individual NIH grants
And grants from VA, NSF, Dept of Energy, etc)
grants)
CT
TG
IG
PG
O
PI: Lazar/
Matchinsky
Clinical Trials
Training Grants
Industrial Grants (including pharmaceuticals)
Private Foundation Grants (including internal PENN
Other
* For program projects, specify whether PI, co-leader, or project leader. For center, core and training grants, similarly specify
your role.** Include any additional, brief information. For clinical trials, for example, specify if multi-center or single center and
indicate role of PENN site. Explain any grants in “Other” category.
Guangping Gao, M.D., Ph.D.
Page 12
Current Grant Support
NAME
Guangping Gao
DEPARTMENT
Pathology and Laboratory Medicine
DATE
2/15/2016
Grant Name & Sponsor
Number
Period of
Award
Grant
Category
*
Role in Grant **
% Effort
Funding
Source
Annual
Direct Cost
P01 A15227
HIV-1 Vaccine based on
chimp serotypes of
adenovirus
040102033107
PP
Project Leader
5%
NIH/NIAID
$205,693
P30 DK47757
Molecular Therapy for CF
and Genetic Disease
090103083108
PP
Project Leader
.6 cal
NIH/NIDDK
$105,000
($666,588)
PI:Wilson
P01 HL051746
Gene Therapy For Cystic
Fibrosis
070104063009
PP
Project Leader
2.4 cal
NIH/NHLBI
$188,313
PI:Crystal
Po1 HL59407
DNA Viruses as Vectors
for Cardiovascular
Diseases
070104063009
PP
Co-Leader
2.4
NIH/NHLBI
$263,034
$1,226,121
PI:Wilson
CFF R881
Research Development
Program – Component III
120103113006
PG
Project Leader
1.2 cal
CF
Foundation
$80,000
($400,000)
PI:Wilson
Additional
Comments
**For Grant Category, use code in bold from the following menu :
R01 NIH R01
PP NIH Program Project, Center or Core Grant
FG Federal Grants – Other (including other individual NIH grants
And grants from VA, NSF, Dept of Energy, etc)
grants)
CT
TG
IG
PG
O
Clinical Trials
Training Grants
Industrial Grants (including pharmaceuticals)
Private Foundation Grants (including internal PENN
Other
* For program projects, specify whether PI, co-leader, or project leader. For center, core and training grants, similarly specify
your role.
** Include any additional, brief information. For clinical trials, for example, specify if multi-center or single center and indicate role
of PENN site. Explain any grants in “Other” category.
Guangping Gao, M.D., Ph.D.
Page 13
Pending Grant Support
NAME
Guangping Gao
Grant Name &
Sponsor
Number
Period of
Award
DEPARTMENT
Grant
Category*
Pathology and Laboratory Medicine
Role in Grant
**
% Effort
Funding
Source
DATE
Annual Direct
Cost
2/15/2016
Additional
Comments
n/a
**For Grant Category, use code in bold from the following menu :
R01 NIH R01
PP NIH Program Project, Center or Core Grant
FG Federal Grants – Other (including other individual NIH grants
And grants from VA, NSF, Dept of Energy, etc)
grants)
CT
TG
IG
PG
O
Clinical Trials
Training Grants
Industrial Grants (including pharmaceuticals)
Private Foundation Grants (including internal PENN
Other
* For program projects, specify whether PI, co-leader, or project leader. For center, core and training grants, similarly specify
your role.
** Include any additional, brief information. For clinical trials, for example, specify if multi-center or single center and indicate role
of PENN site. Explain any grants in “Other” category.