Activities in 2009
Zoonoses in Europe
Prof. Dr Thomas C. Mettenleiter
Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health
Headquarters, Südufer 10, 17493 Greifswald – Isle of Riems, GERMANY
Tel.: (+49-38351) 71.02, Fax: (+49-38351) 71.51
poststelle@fli.bund.de
Summary of activities specifically related to the mandate of
OIE Collaborating Centres
1.
Activities as a centre of research, expertise, standardisation and dissemination of techniques
within the remit of the mandate given by the OIE
Centre of research: The Friedrich-Loeffler-Institut (FLI) is a higher independent Federal authority with the legal
task to perform research on infectious diseases of animals. It houses all national reference laboratories for
notifiable infectious diseases of animals. It is also the national authority to give market authorization for tests for
diagnosis of infectious diseases in animals and participates in epidemiological investigations of animal disease
outbreaks
The FLI is a leading member of the network of excellence 'Epizone' funded by the European Union which brings
together 20 institutes to coordinate research on epizootic diseases of animals. On the national level it coordinates
together with the university of Münster and the Telematikplattform für Medizinische Forschungsnetze e.V. (TMF)
the National Research Platform for Zoonoses. This network bundles the research activities in the field of zoonotic
infectious diseases. Development of flexible and sustainable solutions to strengthen research, prevention, and
therapy of zoonotic infectious diseases are its main objectives.
Bovine tuberculosis/Paratuberculosis: Research project (Deutsche Forschungsgemeinschaft [German Research
Foundation] TA 668/1-1): Mycobacterium bovis in the wildlife-livestock-human interface in East and Southern
Africa. African network building for the control of neglected zoonoses. Research project (EU FP7 – 221948;
ICONZ): Integrated control of neglected zoonoses: improving human health and animal production through
scientific innovation and public engagement.
Participation in the final meeting of the EU Coordination Action VENoMYC (Veterinary Network of Laboratories
Researching into Improved Diagnosis and Epidemiology of Mycobacterial Diseases) in Turin, 17 th-20th June,
2009. Summing up of the workshop “Laboratory diagnosis of Mycobacterium spp.”. Participation in the meeting
of the EU Coordination Action VENoMYC (Veterinary Network of Laboratories Researching into Improved
Diagnosis and Epidemiology of Mycobacterial Diseases) in Madrid, 23rd – 25th March, 2009. VNTR/MIRUs and
DVR spoligotyping for M. bovis typing - Results of the VNTR-DVR Spoligotyping Ring Trial”.
Participation in the meeting of the EU Community Reference Laboratory for Bovine Tuberculosis in Madrid, 5 th –
6th November, 2009
Brucellosis: A second international ring trial for brucella genotyping with Multilocus Variable Number of
Tandem Repeat Analysis (MLVA) was organized by our lab together with Italian colleagues (Istituto
Zooprofilattico Sperimentale "G. Caporale", Teramo). 12 DNA samples were sent out to 15 laboratories in 11
countries and results from 12 labs were received.
Chlamydiae: The Reference Laboratory for Chlamydiosis initiated the drafting of a review article on recent
advances in the laboratory diagnosis of chlamydia infections, which was published in 2009 (Sachse et al. Vet.
Microbiol. 135, 2-21; see list of publications). This paper contains numerous recommendations and comments, all
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of which represent the common position of the leading European researchers in the field of veterinary
chlamydiology
Protocols and technical expertise of real-time PCR detection and DNA microarray tests have been provided to
laboratories in the Netherlands, Switzerland, France, Ireland, Sweden and Argentina. These methods are being
used by diagnostic labs in a number of countries, and some of them have the potential to become standard
procedures.
Echinococcosis: Identification of the red-necked wallaby (Macropus rufogriseus) as an intermediate host of
Echinococcus multilocularis. Studies on the spread and behaviour of the raccoon dog (Nyctereutes procyonoides)
as a new definitive host of Echinococcus multilocularis in eastern and central Europe. After accreditation of the
laboratory according to ISO 17025:2005, the laboratory validated molecular techniques for diagnosis and for the
differentiation of Echinococcus spp. samples and for the distinction of Echinococcus spp. from Taenia spp. eggs.
Contribution to a new edition of the WHO/OIE Manual on Echinococcosis.
Glanders: A protocol of the Western Blot technique for the serological diagnostic was evaluated and compared
with other diagnostic techniques.
Hantavirus: FLI continued the development of diagnostic tools for serological and molecular biological detection
of hantavirus infections in rodents and other small mammals. These techniques were developed in close
collaboration with the Bundeswehr Institute of Microbiology, Munich, the Bernhard-Nocht-Institute for Tropical
Medicine, Hamburg, the Institute of Virology, Charité, Berlin and the Institute of Biotechnology in Vilnius,
Lithuania.
Influenza: Avian Influenza: Research into avian influenza virology in 2009 focussed on molecular tools
enhancing diagnostic capabilities. Studies on the production of recombinant hemagglutinin antigen tailored for use
in subtype-specific ELISAs have been successfully finished. Promising results have also been obtained for a
genetically modified porcine cell line suitable to promote the replication of a wide variety of avian influenza
viruses of low pathogenicity independent of supplementation of the culture medium with trypsin. In addition,
commercial kits for real-time RT LAMP analysis of subtype H5 and H7 viruses have been evaluated; while the
LAMP technology offers advantages particularly for on-site use, the currently available kits were found
significantly less sensitive than the gold standard method, real-time RT PCR.
An OIE twinning project between the National Laboratory for Veterinary Quality Control on Poultry Production
(NLQP), Institute for Animal Health Research (ARI), Cairo, Egypt, and the Friedrich-Loeffler-Institute (FLI), Isle
of Riems, Germany continued. Ongoing collaborations within OFFLU, the OIE/FAO network of expertise on
Avian influenza.
Influenza: New Influenza A/H1N1 “Swineflu”: Research projects in 2009 focussed on disease pathogenesis and
transmissibility studies in pigs. Current activities evaluate commercially available vaccines against influenza virus
infections in pigs for their potential to protect from virus infection, clinical signs, and/or virus shedding.
Newcastle Disease: Research was focussed on NDV in pigeons. Beside molecular characterization and
pathotyping of currently circulating NDV isolates from pigeons a study on prevalence of Avian Paramyxovirus in
wood pigeons (Columba palumbus) was conducted. In addition investigations on immune response of turkeys after
NDV-vaccination were initiated.
The OIE twinning project between the National Laboratory for Veterinary Quality Control on Poultry Production
(NLQP), Institute for Animal Health Research (ARI), Cairo, Egypt, and the Friedrich-Loeffler-Institute (FLI), Isle
of Riems, Germany was ongoing. The program is focused on collaboration for AI and ND diagnosis. This year a
total of seven scientists visited the laboratory for training purposes. Besides national activities for federal agencies
the Laboratory provided assistance for NDV – diagnostic for laboratories from the United Emirates and Ethiopia.
Orthopoxviruses: In 2009, several human infection clusters with cowpox virus (CPXV), transmitted by so-called
pet rats, occurred in Germany and France. Therefore, in a series of animal experiments, pet rats, Wistar rats, and
cattle were infected with a CPXV strain isolated from an infected pet rat. Reference materials from the infected
animals and pathogenesis data were collected. Infection of Wistar rats was established as a suitable infection
model for the recent CPXV.
Rabies: The FLI is responsible for editing the Rabies Bulletin Europe (RBE), in which numbers of reported rabies
cases and rabies surveillance data from all European countries are collected and published quarterly. In 2009, the
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Annual reports of OIE Reference Laboratories and Collaborating Centres, 2009
Zoonoses in Europe
issues 03/2008, 04/2008, 1/2009 and 02/2009 were edited and distributed throughout the world. Data are free to
download and available from our website (http://www.rbe.fli.bund.de/).
Salmonellosis in cattle: Molecular and epidemiological analysis of Salmonella Dublin infections in cattle in
Germany. Epidemiological analysis of bovine salmonellosis according to the data in National Animal Disease
Reporting System in Germany.
TSE: The National TSE Reference Laboratory at the FLI has been in close collaboration with the NRLs of the
other European countries, especially with those of EU members. This is reflected in numerous EU-funded research
projects that have been joined during the last year(s). In the context of these activities FLI has supplied other TSE
NRLs and research groups with several dozens of BSE or scrapie positive samples and reference materials (e.g.
fresh brainstem material or paraffin-embedded fixed tissue). A special emphasis was put on the collaboration with
the Polish NRL regarding the biochemical characterization of the first notified atypical scrapie cases in Poland.
Moreover, the German NRL has been in close contact with the Canadian NRL over the last years and has supplied
this laboratory with ring trial samples in order to support their quality control system.
Tularemia: The National Tularemia Reference Laboratory at the FLI has been in contact with the NRLs of France
and Switzerland as well as other laboratories in Austria, China, Poland and Spain that are involved in the detection
of biological agents. An exchange of strains, techniques and information to evaluate diagnostic and typing tools
took place.
West Nile Fever: The FLI has developed and validated serological (ELISA, Immunofluorescence-assays, microneutralisation test) and molecular biological methods (different quantitative realtime-PCR) for detection of WestNile-Virus (WNV) infections in animals. These techniques were developed in close collaboration with the RobertKoch-Institute in Berlin, the Bernhard-Nocht-Institute for Tropical Medicine in Hamburg and the Paul-EhrlichInstitute in Langen.
The National West-Nile-Virus Reference Laboratory at the FLI has been in contact with the NRLs of France,
Austria, Cyprus, Poland, Switzerland and Romania. Realtime PCR protocols for WNV which were developed by
FLI and evaluated against published methods were supplied to the Swiss WNV NRL in Bern. With the newly
established EU-CRL for equine diseases, AFSSA, Paris, France a vivid exchange of techniques and information to
evaluate a sensitive WNV diagnostic is has been continued. With the NRL in Austria and Poland we exchanged
PCR-protocols, requirements for the safety level 3 laboratories and exchange of information for a better
monitoring study to use as an early-warning system. Furthermore we carry out an intensive advisory service for
the Central Veterinary Service of Cyprus. First efforts for an exchange of serum samples with WNV-antibodies
and virus isolates with the arbovirus NRL in Romania were undertaken.
2.
Proposal or development of any procedure that will facilitate harmonisation of international
regulations applicable to the surveillance and control of animal diseases, food safety or animal
welfare
Bovine tuberculosis/Paratuberculosis: Development of a novel probe based two-target real-time PCR system for
the detection of DNA of members of the M. tuberculosis complex (MTC), including M. bovis and M. caprae, in
tissue samples from cattle and transfer of the protocol to the regional diagnostic laboratories in Germany.
Chlamydiae: RL Chlamydiosis proposes the introduction of an alternative diagnostic reference test for chlamydial
infections to replace cell culture. This procedure includes a validated real-time PCR detection assay in conjunction
with the ArrayTube® microarray test. The proposal is currently being discussed among the chlamydia research
community.
Echinococcosis: Proficiency testing was prepared to compare the performance of different diagnostic techniques
in various laboratories at the international level.
Glanders: In the frame of an EU-Project, the laboratory took part in two international ring trials for antigen
detection of highly infectious agents, including B. mallei and B. pseudomallei together with 21 other European
laboratories. Furthermore, the laboratory took part in the Glanders CFT QAU Proficiency Testing organized by the
VLA, Weybridge.
Influenza: New Influenza A/H1N1 “Swineflu”: Animal experiments also investigated viral dissemination in the
infected organism in order to verify food safety.
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Q fever: The EFSA project "Development of harmonised schemes for monitoring and reporting of rabies and Q
fever in animals in the European Union" has been continued.
Rabies: The FLI together with other 4 European rabies laboratories participated in a project funded by the
European Food Safety Authority (EFSA) on the development of harmonised schemes for monitoring and reporting
of rabies and Q fever in animals in the European Union. Within this project currently existing recommendations on
rabies surveillance of international organisations were reconsidered and based on scientific data the introduction of
a situation-based rabies surveillance both for terrestrial (classical) rabies as well as bat rabies suggested.
Salmonellosis in cattle: Further studies on improving the method for the bacterial detection of salmonellae
according to the national German “Regulation on protection against salmonellosis in cattle” and comparing with
the method according to ISO 6579 Annex D. As result of these activities, the existing method for bacterial
detection of salmonellae according to the national German “Regulation on protection against salmonellosis in
cattle” was replaced by method according to ISO 6579 Annex D.
TSE: In close collaboration with the Community Reference Laboratory for TSEs in Weybridge, UK and other
NRLs, FLI scientists were involved in the establishment of an EU-wide batch control system for rapid tests for the
mass screening of ruminant samples for BSE and scrapie. A major progress has been achieved in this goal, as an
EU-wide batch control system has already been established for four different BSE rapid tests (IDEXX HerdChek
Test, PrioSTRIP Test, Prionics Western Test, BetaPrion BSE EIA Test Kit / ajRoboscreen). The German NRL is
responsible for the EU wide batch control for the TeSeE Test (BioRad), which is currently under preparation and
the start of the EU wide batch control for this test is anticipated for the first quarter of 2010. Moreover, FLI has
contributed to the revisions of the BSE chapter 11.6., the scrapie chapter 14.9., and appendix 3.8.5 of the OIE
Terrestrial Animal Health Standards Commission.
Tularemia: In order to obtain an overview of the prevalence of tularemia in Europe we propose that sentinel
animals (e.g. wild boars) that are tested for other diseases on a routinely basis should be screened serologically.
Serological tests for this purpose need to be evaluated and standardized.
3.
Placement of expert consultants at the disposal of the OIE
Influenza: Avian Influenza: Members of the OIE Reference Laboratory for Avian Influenza at the FLI
contributed to three OFFLU technical groups. Expertise on the availability of commercial AI-related diagnostics,
the production and dissemination of PCR standard controls, and on use of AI vaccines in Indonesia is continuously
provided. In addition, Dr. Harder represented OFFLU at a meeting of the WHO Technical Advisory Group on
molecular influenza diagnosis.
Influenza: New Influenza A/H1N1 “Swine flu”: Dr. Vahlenkamp participated as expert in three meetings
organized by the EU discussing and approving the ‘Working document on surveillance, monitoring and control
measures for the pandemic (H1N1) 2009 influenza virus in pigs and in poultry’.
Newcastle Disease: Revison of the O.I.E. Chapter 2.3.14. NEWCASTLE DISEASE (Editor Claudio L Afonso)
Rabies: Expert opinion on the evaluation of the Rabies Platelia II ELISA test in the frame of the pet travel scheme
Other activities related to the mandate
of OIE Collaborating Centres
4.
Provision of scientific and technical training, within the remit of the mandate given by the OIE, to
personnel from OIE Members
Bovine tuberculosis/Paratuberculosis: Dr. Sodnomdarjaa, Director of the State Central Veterinary Laboratory,
Mongolia, stayed in the FLI for consultation about diagnosis of bovine tuberculosis and paratuberculosis.
Brucellosis: One responsible veterinarian from the NRL Lithuania was trained for one week in serological
methods. Two scientists from University of Veterinary & Animal Science, Lahore, Pakistan and from the National
Research Center, Cairo, Egypt underwent a three-month training on laboratory diagnosis of brucellosis.
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Annual reports of OIE Reference Laboratories and Collaborating Centres, 2009
Zoonoses in Europe
Chlamydiae: In 2009, researchers and lab diagnosticians from France, Italy, Belgium and Sweden visited the
reference lab for technical training and consultations on chlamydial diagnostic methods, with emphasis on
molecular detection methods.
Glanders: One PHD student from University of Veterinary & Animal Science, Lahore, Pakistan started the work
in the field of “Molecular Seroepidemiology of Glanders”. Veterinarians from Brazil, Poland, Spain and UK
stayed each for a two-weeks training on laboratory diagnosis of glanders.
Hantavirus: In the frame of the network “Rodent-borne pathogens” FLI has a close collaboration with a large
number of groups in Germany. These collaborations are mainly driven by the exchange of materials for the
surveillance of infections with hantaviruses and other zoonotic pathogens such as Ljungan virus, orthopox viruses,
tick-borne encephalitis virus, Borrelia spp., Coxiella burnetii, Leptospira spp., E. coli and other bacterial
pathogens, and endoparasites. Serological and molecular biological studies on the orthopox virus prevalence in
wild rodents from Germany were performed in a close collaboration with the Haartman Institute, Helsinki,
Finland. For training purposes young scientists from other laboratories in Germany were involved in the
hantavirus investigations.
Influenza: Avian Influenza: Within the OIE twinning project extensive training visits of staff have been
organized. Training on the job has been provided at the FLI for seven colleagues from the NLQP. Further training
was provided for colleagues from Bangladesh and Russia.
Newcastle Disease: A one year training program for Dr. Fufa Dawo Bari, a scientist from the university of Adis
Abeba, Ethiopia was ongoing. He left the Laboratory on September 5th 2009. Issues of training comprised
molecular and classical techniques of NDV diagnosis. During the second half of the training the focus was on
phylogenic analysis of NDV-isolates. As part of the OIE twinning project with the NLQP, Egypt scientists were
trained on classical diagnostic methods (2 week program), on production of reference materials (4 week program)
and pathogenicity testing (4 weeks program).
Q fever: A training in diagnostic methods for the detection of Q fever was performed for colleagues from Spain
and Poland.
Rabies: Training of scientific and technical staff: In 2009 we hosted a number of colleagues from other OIE
member countries including one person from Lithuania, two persons from Ukraine, two persons from Turkey, two
persons from Mongolia. The training included all standard rabies diagnostic tests, e.g. rabies diagnosis, rabies
serology, follow-up investigations of oral vaccination campaigns and virus characterization (virus typing,
sequencing, rabies surveillance, evaluation of ORV campaigns).
TSE: FLI has been particularly contacted by NRLs of new member states to get assistance in elaborating and
establishing the diagnostic routine. In the past colleagues from FLI have participated in Twinning Projects (one of
these projects was lead by the FLI) and have developed an excellent working relationship with scientists in these
countries. Several follow up visits and visits to other eastern European NRLs have therefore taken place e.g. in
Sofia, Bulgaria, and in Pulawy, Poland. Moreover, colleagues from Bulgaria, Poland and Turkey have stayed in
the FLI laboratory for extended visits in order to gain a thorough insight into the diagnostic and confirmatory work
as it is performed at the FLI-associated TSE National Reference Laboratory.
Tularämie: Short term training in conventional and molecular diagnostic procedures was provided for scientists
from China and Egypt.
5.
Organisation of scientific meetings on behalf of the OIE
Bovine tuberculosis/Paratuberculosis: Organisation of the 4th Workshop on Mycobacterial Infections on 13 th14th May, 2009, in Jena with participants from Germany, Austria, GB, Switzerland, Luxembourg, The Netherlands
and France.
TBE and other Tick-borne Diseases: The Xth International Jena Symposium on Tick-borne Diseases (IJSTD-X)
was held in Weimar, 19-21 march 2009.
The very high participation (more than 300 participants from 31 countries) and the large number of presentations
(52 oral and 112 poster presentations) have shown that the Symposium promises to thrive at the new venue
Weimar. We chose PREVENTION as the main topic for this anniversary symposium, a theme that summarizes the
ultimate aims of all our efforts, i.e. to prevent tick bites and cases of tick-borne disease. In addition to
Annual report of OIE Reference Laboratories and Collaborating Centres, 2009
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Zoonoses in Europe
PREVENTION, all fields of research on ticks, tick-borne pathogens, and tick-borne diseases were dealt with
during the meeting
Süss, J., Kahl, O. (2009): X International Jena Symposium on Tick-borne Diseases: Book of Abstracts, pp. 1-190
www.TBD-SYMPOSIUM.COM
6.
Coordination of scientific and technical studies in collaboration with other laboratories,
organisations or collaborating centres
Bovine tuberculosis/Paratuberculosis: Organisation of an inter-laboratory ring trial regarding detection of
Mycobacterium avium ssp. paratuberculosis in bovine faecal samples with bacteriological and molecular
biological methods (Germany, Austria, Luxembourg, The Netherlands) – summing up.
Organisation of an inter-laboratory ring trial regarding detection of Mycobacterium bovis in bovine tissue samples
with molecular-biological methods (Germany).
Organisation of an inter-laboratory ring trial regarding isolation, cultivation and identification of different
mycobacterial species (Germany, Austria, Spain).
Brucellosis: Two representatives of the OIE reference laboratory for brucellosis (Dr. Neubauer and Dr. Melzer)
were members of the scientific workgroup of the EFSA “Brucellosis in pigs”. It was founded to provide scientific
advice on the risks of Brucella suis being introduced into domestic pig herds as a result of the new
epidemiological situation, the suitability of current and new tests for porcine brucellosis and existing strategies to
control or eradicate this disease. The outcome of the work was published in the EFSA Journal.
Chlamydiae: A joint validation study examining the use of a new DNA microarray-based test for identification of
Chlamydophila psittaci genotypes (serovars) was conducted with AFSSA, Unité Zoonoses Bactériennes, MaisonsAlfort, France (see 7.7 below).
Another collaborative study focused on testing of samples from wild bird monitoring in Switzerland to
characterise the prevalence of chlamydiae in wild bird populations (partners: University of Zurich; see 7.9 below).
Hantavirus: The group at the FLI continued the coordination of the network “rodent-borne pathogens” and
recruited novel partners working on different rodent-associated pathogens such as retroviruses and endoparasites
or rodent phylogeography. Besides the long time running studies on wild rodents, investigations on commensal
rodents such as house mouse and Norway rat were intensified together with several novel network partners.
Moreover, longitudinal studies on rodent population dynamics were initiated together with a group at the Julius
Kühn-Institut, Münster.
Rabies: Research activities were finished to experimentally investigate the host response to infection with
European bat lyssavirus types 1 in Serotine bats (Eptesicus serotinus) (collaboration with VLA, UK; CDC, USA;
Pasteur Institute, France). The FLI continued work within a WHO co-ordinated research project on research and
development on a murine monoclonal antibody (MAbs) cocktail to replace HRIG and ERIG for rabies postexposure prophylaxis (PEP) in humans
The OIE reference laboratory is partner in the EU funded EPIZONE project, work package WILDSURV
harmonization, with special emphasis on rabies surveillance. Also, the FLI together with other 4 European rabies
laboratories participated in a project funded by the European Food Safety Authority (EFSA) on the development
of harmonised schemes for monitoring and reporting of rabies and Q fever in animals in the European Union.
TSE: FLI has collaborated with the Polish NRL for TSEs to characterise atypical and scrapie BSE cases.
Moreover, FLI has worked together with the Central Veterinary Service of Cyprus on the diagnosis and
pathogenesis of TSE diseases in Cypriot sheep and goats.
7.
Publication and dissemination of any information within the remit of the mandate given by the
OIE that may be useful to Members of the OIE
Hoffmann, B., M. Beer, S. M. Reid, P. Mertens, C. A. Oura, P. A. van Rijn, M. J. Slomka, J. Banks, I. H. Brown, D. J.
Alexander, and D. P. King. 2009. A review of RT-PCR technologies used in veterinary virology and disease control: Sensitive
and specific diagnosis of five livestock diseases notifiable to the World Organisation for Animal Health. Veterinary
Microbiology 139:1-23
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Zoonoses in Europe
Thulke, H. H., D. Eisinger, C. Freuling, A. Frohlich, A. Globig, V. Grimm, T. Muller, T. Selhorst, C. Staubach, and S. Zips.
2009. Situation-Based Surveillance: Adapting Investigations to Actual Epidemic Situations. Journal of Wildlife Diseases
45:1089-1103.
Bovine tuberculosis/Paratuberculosis: Bauer, K., Kummer, C., and Moser, I. VNTR-typing of Animal Mycobacterium
tuberculosis Complex Isolates using 24 Loci – Experiences in the German Tuberculosis Reference Laboratory. Proceedings of
the VENoMYC WP7 "VNTR/MIRUs and DVR spoligotyping for M.bovis typing", 9. 2009.
Borrmann, E., Möbius, P., and Köhler, H. Different cytokine responses after interaction between human macrophages and
Mycobacterium avium subsp. paratuberculosis type II and type III strains. Proceedings of the 10th International Colloquium on
Paratuberculosis (ICP). August 9th -14th, 2009, Minneapolis, Minnesota. In press.
Elze, J., Köhler, H., and Liebler-Tenorio, E. The suitability of macroscopic preselection for prevalence estimation of bovine
paratuberculosis at the slaughterhouse. Proceedings of the 10th International Colloquium on Paratuberculosis (ICP). August 9th
-14th, 2009, Minneapolis, Minnesota. In press.
Fritsch, I., Köhler, H., and Möbius, P. Comparative characterization from German isolates of Mycobacterium avium subsp.
paratuberculosis using SSR-, IS900-RFLP- and MIRU-VNTR-analysis. Proceedings of the 10th International Colloquium on
Paratuberculosis (ICP). August 9th -14th, 2009, Minneapolis, Minnesota. In press.
Gierke, F. and H. Kohler. 2009. Tenacity, excretion and transmission of Mycobacterium paratuberculosis - Consequences for
the control of paratuberculosis in cattle herds. Praktische Tierarzt 90:1169-1178.
Gierke, F., Ziller, M., and Köhler, H. Multinomial regression analysis of individual host factors and paratuberculosis test
results. Proceedings of the 10th International Colloquium on Paratuberculosis (ICP). August 9th -14th, 2009, Minneapolis,
Minnesota. In press.
Knobloch, H., Commander, N., Reinhold, P., Turner, C., Spooner, A., Chambers, M., and Köhler, H. Analysis of volatile
organic compounds in sera - a future prospect of paratuberculosis diagnosis? Proceedings of the 10th International Colloquium
on Paratuberculosis (ICP). August 9th -14th, 2009, Minneapolis, Minnesota. In press.
Knobloch, H., H. Köhler, N. Commander, P. Reinhold, C. Turner, and M. Chambers. 2009. Volatile Organic Compound
(VOC) Analysis For Disease Detection: Proof Of Principle For Field Studies Detecting Paratuberculosis And Brucellosis In M.
Pardo and G. Sberveglieri (eds.), CP1137, Olfaction and Electronic Nose: Proceedings of the 13th International Symposium.
American Institute of Physics.
Köhler, H., F. Gierke, T. Seidler, and M. Ziller. Control of paratuberculosis by vaccination - a systematic review and metaanalysis. Proceedings of the 10th International Colloquium on Paratuberculosis (ICP). August 9th -14th, 2009, Minneapolis,
Minnesota. In press.
Möbius, P., I. Fritsch, G. Luyven, H. Hotzel, and H. Köhler. 2009. Unique genotypes of Mycobacterium avium subsp
paratuberculosis strains of Type III. Veterinary Microbiology 139:398-404
Moser, I. The Challenge of Dourine Diagnostics - Actual Methods Performed in the German Reference Laboratory, Proposals
for Future Work. Proceedings of the 1st Workshop of the EU CRL for Dourine, AFSSA-LERPAZ Maisons-Alfort, France,
10th December 2009 . 2009. 10-12-2009.
Brucellose: Al Dahouk, S., S. Loisel-Meyer, H. C. Scholz, H. Tomaso, M. Kersten, A. Harder, H. Neubauer, S. Kohler, and V.
Jubier-Maurin. 2009. Proteomic analysis of Brucella suis under oxygen deficiency reveals flexibility in adaptive expression of
various pathways. Proteomics 9:3011-3021
Maquart, M., P. Le Fleche, G. Foster, M. Tryland, F. Ramisse, B. Djonne, S. Al Dahouk, I. Jacques, H. Neubauer, K.
Walravens, J. Godfroid, A. Cloeckaert, and G. Vergnaud. 2009. MLVA-16 typing of 295 marine mammal Brucella isolates
from different animal and geographic origins identifies 7 major groups within Brucella ceti and Brucella pinnipedialis. Bmc
Microbiology 9:
Scholz, H. C., K. Nöckler, C. Göllner, P. Bahn, G. Vergnaud, H. Tomaso, S. Al Dahouk, P. Kämpfer, A. Cloeckaert, M.
Maquart, M. S. Zygmunt, A. M. Whatmore, M. Pfeffer, B. Huber, H. J. Busse, and K. De Barun. Brucella inopinata sp. nov.,
isolated from a breast implant infection International Journal of Systematic and Evolutionary Microbiology,
doi:10.1099/ijs.0.011148-0
Scholz, H. C., E. Hofer, G. Vergnaud, P. Le Fleche, A. M. Whatmore, S. Al Dahouk, M. Pfeffer, M. Kruger, A. Cloeckaert, and
H. Tomaso. 2009. Isolation of Brucella microti from Mandibular Lymph Nodes of Red Foxes, Vulpes vulpes, in Lower
Austria. Vector-Borne and Zoonotic Diseases 9:153-155
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Schulze zur Wiesch, J., D. Wichmann, I. Sobottka, H. Rohde, G. Schmoock, R. Wernery, S. Schmiedel, G. D. Burchard, and F.
Melzer. 2009. Genomic Tandem Repeat Analysis Proves Laboratory-Acquired Brucellosis in Veterinary (Camel) Diagnostic
Laboratory in the United Arab Emirates. Zoonoses and Public Health doi: 10.1111/j.1863-2378.2009.01258.x
Thoma, B., E. Straube, H. C. Scholz, S. Al Dahouk, L. Zoller, M. Pfeffer, H. Neubauer, and H. Tomaso. 2009. Identification
and antimicrobial susceptibilities of Ochrobactrum spp. International Journal of Medical Microbiology 299:209-220
Chlamydiae: Gaede, W., K. F. Reckling, A. Schliephake, D. Missal, H. Hotzel, and K. Sachse. 2009. Detection of
Chlamydophila caviae and Streptococcus equi subsp. zooepidemicus in horses with signs of rhinitis and conjunctivitis. Vet.
Microbiol. doi:10.1016/j.vetmic.2009.10.011:
Kühlewind, S., Sachse, K, Schubert, E., Ulbrich, M., Schärling, B., Matzner, N. et al. (2009) Patterns of zoonotic exposure to
chlamydia and their impact on acute and/or persistent pulmonary morbidity. Eur. Respir. J. 2009, 34.
Laroucau, K., B. de Barbeyrac, F. Vorimore, M. Clerc, C. Bertin, T. Harkinezhad, K. Verminnen, F. Obeniche, I. Capek, C.
Bebear, B. Durand, G. Zanella, D. Vanrompay, B. Garin-Bastuji, and K. Sachse. 2009. Chlamydial infections in duck farms
associated with human cases of psittacosis in France. Veterinary Microbiology 135:82-89
Laroucau, K., F. Vorimore, C. Bertin, K. Y. Mohamad, S. Thierry, W. Hermann, C. Maingourd, C. Pourcel, D. Longbottom, S.
Magnino, K. Sachse, E. Vretou, and A. Rodolakis. 2009. Genotyping of Chlamydophila abortus strains by multilocus VNTR
analysis. Veterinary Microbiology 137:335-344
Laroucau, K., F. Vorimore, R. Aaziz, A. Berndt, E. Schubert, and K. Sachse. 2009. Isolation of a new chlamydial agent from
infected domestic poultry coincided with cases of atypical pneumonia among slaughterhouse workers in France. Infection,
Genetics and Evolution 9:1240-1247
Pantchev, A., R. Sting, R. Bauerfeind, J. Tyczka, and K. Sachse. 2009. Detection of all Chlamydophila and Chlamydia spp. of
veterinary interest using species-specific real-time PCR assays. Comp Immunol. Microbiol. Infect. Dis.
doi:10.1016/j.cimid.2009.08.002.
Pantchev, A., R. Sting, R. Bauerfeind, J. Tyczka, and K. Sachse. 2009. New real-time PCR tests for species-specific detection
of Chlamydophila psittaci and Chlamydophila abortus from tissue samples. Veterinary Journal 181:145-150.
Reinhold, P., H. Hartmann, and P. D. Constable. 2009. Characterisation of acid-base abnormalities in pigs experimentally
infected with Chlamydia suis. Vet. J. doi:10.1016/j.tvjl.2009.02.005
Sachse, K., E. Vretou, M. Livingstone, N. Borel, A. Pospischil, and D. Longbottom. 2009. Recent developments in the
laboratory diagnosis of chlamydial infections. Veterinary Microbiology 135 :2-21.
Sachse, K., K. Laroucau, F. Vorimore, S. Magnino, J. Feige, W. Muller, S. Kube, H. Hotzel, E. Schubert, P. Slickers, and R.
Ehricht. 2009. DNA microarray-based genotyping of Chlamydophila psittaci strains from culture and clinical samples.
Veterinary Microbiology 135:22-30.
Sprague, L. D., E. Schubert, H. Hotzel, S. Scharf, and K. Sachse. 2009. The detection of Chlamydophila psittaci genotype C
infection in dogs. Veterinary Journal 181:274-279
Zweifel, D., R. Hoop, K. Sachse, A. Pospischil, and N. Borel. 2009. Prevalence of Chlamydophila psittaci in wild birds potential risk for domestic poultry, pet birds, and public health? European Journal of Wildlife Research 55:575-581
Echinococcosis: Peters, M. K. J. W. P. C. F. J. 2009. Alveolar echinococcosis in a captive red-necked wallaby (Macropus
rufogriseus). Berl. Münch. Tierärztl. Wochenschr (in press)
Pitra, C., S. Schwarz, and J. Fickel . 2009. Going west - invasion genetics of the alien raccoon dog Nyctereutes procynoides in
Europe. European Journal of Wildlife Research 55: DOI 10.1007/s10344-009-0283-2
Sutor, A. K. K. A. H. 2010. Diet of the raccoon dog (Nyctereutes procyonoides) – a canid with an opportunistic foraging
strategy. Acta Theriologica (in press)
Glanders: Sprague, L. D., R. Zachariah, H. Neubauer, R. Wernery, M. Joseph, H. C. Scholz, and U. Wernery. 2009.
Prevalence-dependent use of serological tests for diagnosing glanders in horses. BMC Veterinary Research 5: 32; DOI:
10.1186/1746-6148-5-32
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Zoonoses in Europe
Yen, M. W. S., O. Lisanti, F. Thibault, T. S. San, L. G. Kee, V. Hilaire, L. Jiali, H. Neubauer, G. Vergnaud, and V. Ramisse.
2009. Validation of ten new polymorphic tandem repeat loci and application to the MLVA typing of Burkholderia
pseudomallei isolates collected in Singapore from 1988 to 2004. Journal of Microbiological Methods 77:297-301
Hantavirus: Chandy, S., M. Okumura, K. Yoshimatsu, R. G. Ulrich, G. T. John, P. Abraham, J. Arikawa, and G. Sridharan.
2009. Hantavirus species in India: A retrospective study. Journal of Medical Microbiology 27:348-350
Essbauer, S., S. Schex, W. Splettstoesser, M. Pfeffer, R. G. Ulrich, E. Seibold, G. Dobler, R. Wölfel, and W. Bäumler. 2009.
Nagetier-übertragene Zoonosen: Beispiele aus Untersuchungen in Süd- und Westdeutschland, p. 37-48. In J. Jacob (ed.),
Wirbeltierforschung in der Kulturlandschaft - Grundlagen und Anwendung Zur Verabschiedung von Dr. Hans-Joachim Pelz in
den Ruhestand, vol. 421.
Mertens, M., R. Wölfel, K. Ullrich, K. Yoshimatsu, J. Blumhardt, I. Römer, J. Esser, J. Schmidt-Chanasit, M. H. Groschup, G.
Dobler, S. S. Essbauer, and R. G. Ulrich. 2009. Seroepidemiological study in a Puumala virus outbreak area in South-East
Germany. Medical Microbiology and Immunology 198:83-91
Schlegel, M., B. Klempa, B. Auste, M. Bemmann, J. Schmidt-Chanasit, T. Buchner, M. H. Groschup, M. Meier, A. BalkemaBuschmann, H. Zoller, D. H. Kruger, and R. G. Ulrich. 2009. Dobrava-Belgrade Virus Spillover Infections, Germany.
Emerging Infectious Diseases 15:2017-2020
Schmidt-Chanasit, J., A. Bialonski, P. Heinemann, R. G. Ulrich, S. Günther, H. F. Rabenau, and H. W. Doerr. 2009. A 10-year
molecular survey of herpes simplex virus type 1 in Germany demonstrates a stable and high prevalence of genotypes A and B.
Journal of Clinical Virology 44:235-237
Ulrich, R. G., G. Heckel, H.-J. Pelz, L. H. Wieler, M. Nordhoff, G. Dobler, J. Freise, F.-R. Matuschka, J. Jacob, J. SchmidtChanasit, F. W. Gerstengarbe, T. Jäkel, J. Süss, B. Ehlers, A. Nitsche, R. Kallies, R. Johne, S. Günther, K. Henning, R.
Grunow, M. Wenk, L. C. Maul, K.-P. Hunfeld, R. Wölfel, G. Schares, H. C. Scholz, S. O. Brockmann, M. Pfeffer, and S. S.
Essbauer. 2009. Nagetiere und Nagetier-assoziierte Krankheitserreger - Das Netzwerk "Nagetier-übertragene Pathogene" stellt
sich vor. Bundesgesundheitsbl - Gesundheitsforsch - Gesundheitsschutz 52:352-369
Influenza:
Avian
Influenza: Scientific and Technical
Including the following contributions from the FLI:
Review
28
(1),
2009
by
Thomas
C.
Mettenleiter
Artois, M., D. Bicout, D. Doctrinal, R. Fouchier, D. Gavier-Widen, A. Globig, W. Hagemeijer, T. Mundkur, V. Munster, and B. Olsen. 2009.
Outbreaks of highly pathogenic avian influenza in Europe: the risks associated with wild birds. Revue Scientifique et Technique-Office
International des Epizooties 28:69-92
Fuchs, W., A. Romer-Oberdorfer, J. Veits, and T. C. Mettenleiter. 2009. Novel avian influenza virus vaccines. Rev. Sci. Tech. 28:319-332.
Matrosovich, M., J. Stech, and H. D. Klenk. 2009. Influenza receptors, polymerase and host range. Revue Scientifique et Technique-Office
International des Epizooties 28:203-217
Rudolf, M., M. Poppel, A. Frohlich, T. Mettenleiter, M. Beer, and T. Harder. 2009. Efficacy of a commercial inactivated H5 influenza vaccine
against highly pathogenic avian influenza H5N1 in waterfowl evaluated under field conditions. Rev. Sci. Tech. 28:275-291.
Fereidouni, S. R., E. Starick, C. Grund, A. Globig, T. C. Mettenleiter, M. Beer, and T. Harder. 2009. Rapid molecular
subtyping by reverse transcription polymerase chain reaction of the neuraminidase gene of avian influenza A viruses.
Veterinary Microbiology 135:253-260
Fereidouni, S., E. Starick, M. Beer, H. Wilking, D. Kalthoff, C. Grund, R. Häuslaigner, A. Breithaupt, E. Lange, and T. Harder.
2009. Highly Pathogenic Avian Influenza Virus (H5N1) Infection of Mallards with homo- (H5) and heterosubtypic (H4)
Immunity induced by Low Pathogenic Avian Influenza Virus. PLoS one 4:
Gall, A., B. Hoffmann, T. Harder, C. Grund, D. Hoper, and M. Beer. 2009. Design and Validation of a Microarray for
Detection, Hemagglutinin Subtyping, and Pathotyping of Avian Influenza Viruses. Journal of Clinical Microbiology 47:327334
Gall, A., B. Hoffmann, T. Harder, C. Grund, R. Ehricht, and M. Beer. 2009. Rapid and Highly Sensitive Neuraminidase
Subtyping of Avian Influenza Viruses by Use of a Diagnostic DNA Microarray. Journal of Clinical Microbiology 47:29852988
Gall, A., B. Hoffmann, T. Harder, C. Grund, R. Ehricht, and M. Beer. 2009. Rapid haemagglutinin subtyping and pathotyping
of avian influenza viruses by a DNA microarray. Journal of Virological Methods 160:200-205
Annual report of OIE Reference Laboratories and Collaborating Centres, 2009
9
Zoonoses in Europe
Globig, A., A. Baumer, S. Revilla-Fernandez, M. Beer, E. Wodak, M. Fink, N. Greber, T. C. Harder, H. Wilking, I. Brunhart,
D. Matthes, U. Kraatz, P. Strunk, W. Fiedler, S. R. Fereidouni, C. Staubach, F. J. Conraths, C. Griot, T. C. Mettenleiter, and K.
D. C. Stark. 2009. Ducks as Sentinels for Avian Influenza in Wild Birds. Emerging Infectious Diseases 15:1633-1636
Globig, A., C. Staubach, M. Beer, U. Koppen, W. Fiedler, M. Nieburg, H. Wilking, E. Starick, J. P. Teifke, O. Werner, F.
Unger, C. Grund, C. Wolf, H. Roost, F. Feldhusen, F. J. Conraths, T. C. Mettenleiter, and T. C. Harder. 2009. Epidemiological
and Ornithological Aspects of Outbreaks of Highly Pathogenic Avian Influenza Virus H5N1 of Asian Lineage in Wild Birds in
Germany, 2006 and 2007. Transboundary and Emerging Diseases 56:57-72
Harder, T. C. and T. W. Vahlenkamp. 2009. Influenza virus infections in dogs and cats. Vet. Immunol. Immunopathol.
Harder, T. C., J. Teuffert, E. Starick, J. Gethmann, C. Grund, S. Fereidouni, M. Durban, K. H. Bogner, A. Neubauer-Juric, R.
Repper, A. Hlinak, A. Engelhardt, A. Nockler, K. Smietanka, Z. Minta, M. Kramer, A. Globig, T. C. Mettenleiter, F. J.
Conraths, and M. Beer. 2009. Highly Pathogenic Avian Influenza Virus (H5N1) in Frozen Duck Carcasses, Germany, 2007.
Emerging Infectious Diseases 15:272-279
Höper, D., B. Hoffmann, and M. Beer. 2009. Simple, Sensitive, and Swift Sequencing of Complete H5N1 Avian Influenza
Virus Genomes. Journal of Clinical Microbiology 47:674-679
Kalhoro, N. H., J. Veits, S. Rautenschlein, and G. Zimmer. 2009. A recombinant vesicular stomatitis virus replicon vaccine
protects chickens from highly pathogenic avian influenza virus (H7N1). Vaccine 27:1174-1183
Kalthoff, D., A. Breithaupt, B. Helm, J. P. Teifke, and M. Beer. 2009. Migratory status is not related to the susceptibility to
HPAIV H5N1 in an insectivorous passerine species. PLoS one 4 :
Kreibich, A., J. Stech, T. C. Mettenleiter, and O. Stech . 2009. Simultaneous one-tube full-length amplification of the NA, NP,
M, and NS genes of influenza A viruses for reverse genetics. Journal of Virological Methods 159:308-310
Michael, K., T. C. Harder, T. C. Mettenleiter, and A. Karger. 2009. Diagnosis and strain differentiation of avian influenza
viruses by restriction fragment mass analysis. Journal of Virological Methods 158:63-69
Pannwitz, G., C. Wolf, and T. Harder. 2009. Active Surveillance for Avian Influenza Virus Infection in Wild Birds by Analysis
of Avian Fecal Samples from the Environment. Journal of Wildlife Diseases 45:512-518
Pavlova, S. P., J. Veits, G. M. Keil, T. C. Mettenleiter, and W. Fuchs. 2009. Protection of chickens against H5N1 highly
pathogenic avian influenza virus infection by live vaccination with infectious laryngotracheitis virus recombinants expressing
H5 hemagglutinin and N1 neuraminidase. Vaccine 27:773-785
Pavlova, S. P., J. Veits, T. C. Mettenleiter, and W. Fuchs. 2009. Live vaccination with an H5-hemagglutinin-expressing
infectious laryngothracheitis virus recombinant protects chickens against different highly pathogenic avian influenza viruses of
the H5 subtype. Vaccine 27:5085-5090
Schoene, C., T. Harder, A. Globig, M. Beer, F. J. Conraths, and T. C. Mettenleiter. 2009. The wild bird enigma: what role do
wild birds play in the highly pathogenic avian influenza epizootic? Tieraerztliche Umschau 64:77-83
Schroer, D., J. Veits, C. Grund, M. Dauber, G. Keil, H. Granzow, T. C. Mettenleiter, and A. Romer-Oberdorfer. 2009.
Vaccination with Newcastle Disease Virus Vectored Vaccine Protects Chickens Against Highly Pathogenic H7 Avian
Influenza Virus. Avian Diseases 53:190-197
Stech, O., J. Veits, S. Weber, D. Deckers, D. Schroer, T. W. Vahlenkamp, A. Breithaupt, J. Teifke, T. C. Mettenleiter, and J.
Stech. 2009. Acquisition of a Polybasic Hemagglutinin Cleavage Site by a Low-Pathogenic Avian Influenza Virus Is Not
Sufficient for Immediate Transformation into a Highly Pathogenic Strain. Journal of Virology 83:5864-5868
Werner, O., T. Harder, J. Veits, A. Römer-Oberdörfer, W. Fuchs, M. Beer, F. J. Conraths, and T. C. Mettenleiter. 2009. Avian
Influenza Outbreaks in Germany – Development of New Avian Vaccines. In H. D. Klenk, M. Matrosovich, and J. Stech (eds.),
Influenza Monogr. Virol. 27. Karger, Basel.
New Influenza A/H1N1 “Swineflu”: Fereidouni, S. R., M. Beer, T. Vahlenkamp, and E. Starick. 2009. Differentiation of
two distinct clusters among currently circulating influenza A(H1N1)v viruses, March-September 2009. Euro. Surveill 14:
Lange, E., D. Kalthoff, U. Blohm, J. P. Teifke, A. Breithaupt, C. Maresch, E. Starick, S. Fereidouni, B. Hoffmann, T. C.
Mettenleiter, M. Beer, and T. W. Vahlenkamp. 2009. Pathogenesis and transmission of the novel swine-origin influenza virus
A/H1N1 after experimental infection of pigs. J. Gen. Virol. 90:2119-2123
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Annual reports of OIE Reference Laboratories and Collaborating Centres, 2009
Zoonoses in Europe
Newcastle Disease: Hauslaigner, R., J. Sonnenburg, S. Kothlow, B. Kaspers, C. Staubach, and C. Grund. 2009. Evaluation of
an indirect enzyme-linked immunosorbent assay to study the specific humoral immune response of Muscovy ducks (Cairina
moschata) and domestic geese (Anser anser var. domestica) after vaccination against Newcastle disease virus. Avian Pathology
38:89-95
Q fever: Henning, K., H. Hotzel, M. Peters, P. Walter, W. Popp, and D. Theegarten. 2009. Unanticipated outbreak of Q fever
during a study using sheep, and its significance for further projects. Berliner und Munchener Tierarztliche Wochenschrift
122:13-19.
Kittelberger, R., J. Mars, G. Wibberley, R. Sting, K. Henning, G. W. Horner, K. M. Garnett, M. J. Hannah, J. A. Jenner, C. J.
Pigott, and J. S. O'Keefe. 2009. Comparison of the Q-fever complement fixation test and two commercial enzyme-linked
immunosorbent assays for the detection of serum antibodies against Coxiella burnetii (Q-fever) in ruminants:
Recommendations for use of serological tests on imported animals in New Zealand. New Zealand Veterinary Journal 57:262268.
Neubauer, H. 2009. Q-Fever. Berliner und Munchener Tierarztliche Wochenschrift 122:471-473
Rabies: Beckert, A., L. Geue, A. Vos, A. Neubert, C. Freuling, and T. Müller. 2009. Genetic stability (in vivo) of the
attenuated oral rabies virus vaccine SAD B19. Microbiology and Immunology 53 :16-21.
Fooks, A. R., N. Johnson, C. M. Freuling, P. R. Wakeley, A. C. Banyard, L. M. McElhinney, D. A. Marston, A. Dastjerdi, E.
Wright, R. A. Weiss, and T. Muller. 2009. Emerging Technologies for the Detection of Rabies Virus: Challenges and Hopes in
the 21st Century. Plos Neglected Tropical Diseases 3.
Fooks, A. R., N. Johnson, T. Müller, A. Vos, K. Mansfield, D. Hicks, A. Nunez, C. Freuling, L. Neubert, I. Kaipf, A.
Denzinger, R. Franka, and C. E. Rupprecht. 2009. Detection of High Levels of European Bat Lyssavirus Type-1 Viral RNA in
the Thyroid Gland of Experimentally-Infected Eptesicus fuscus Bats. Zoonoses and Public Health 56:270-277.
Freuling, C., A. Vos, N. Johnson, A. R. Fooks, and T. Muller. 2009. Bat Rabies - a Gordian knot? Berliner und Munchener
Tierarztliche Wochenschrift 122:425-433.
Freuling, C., A. Vos, N. Johnson, I. Kaipf, A. Denzinger, L. Neubert, K. Mansfield, D. Hicks, A. Nunez, N. Tordo, C. E.
Rupprecht, A. R. Fooks, and T. Müller. 2009. Experimental infection of Serotine bats (Eptesicus serotinus) with European bat
lyssavirus type 1a (EBLV-1a). Journal of General Virology 90:2493-2502.
Johnson, N., H. Un, A. R. Fooks, C. Freuling, T. Muller, O. Aylan, and A. Vos. 2009. Rabies epidemiology and control in
Turkey: past and present. Epidemiol. Infect. doi:10.1017/S0950268809990963:1-8
Malerczyk, C., T. Selhorst, N. Tordo, S. Moore, and T. Muller. 2009. Antibodies induced by vaccination with purified chick
embryo cell culture vaccine (PCECV) cross-neutralize non-classical bat lyssavirus strains. Vaccine 27:5320-5325.
Marston, D. A., L. M. McElhinney, Y. H. Ali, K. S. Intisar, S. M. Ho, C. Freuling, T. Müller, and A. R. Fooks. 2009.
Phylogenetic analysis of rabies viruses from Sudan provides evidence of a viral clade with a unique molecular signature. Virus
Research 145:244-250.
Muller, T., B. Dietzschold, H. Ertl, A. R. Fooks, C. Freuling, C. Fehlner-Gardiner, J. Kliemt, F. X. Meslin, C. E. Rupprecht, N.
Tordo, A. I. Wanderler, and M. P. Kieny. 2009. Development of a Mouse Monoclonal Antibody Cocktail for Post-exposure
Rabies Prophylaxis in Humans. Plos Neglected Tropical Diseases 3.
Müller, T., H. J. Bätza, A. Beckert, C. Bunzenthal, J. H. Cox, C. M. Freuling, A. R. Fooks, J. Frost, L. Geue, A. Hoeflechner,
D. Marston, A. Neubert, L. Neubert, S. Revilla-Fernandez, E. Vanek, A. Vos, E. Wodak, K. Zimmer, and T. C. Mettenleiter.
2009. Analysis of vaccine-virus-associated rabies cases in red foxes (Vulpes vulpes) after oral rabies vaccination campaigns in
Germany and Austria. Archives of Virology 154:1081-1091.
Pitra, C., S. Schwarz, and J. Fickel. 2009. Going west - invasion genetics of the alien raccoon dog Nyctereutes procynoides in
Europe. European Journal of Wildlife Research 55.
Ramnial, V., R. Kosmider, O. Aylan, C. Freuling, T. Muller, and A. R. Fooks. 2009. Quantitative risk assessment to compare
the risk of rabies entering the UK from Turkey via quarantine, the Pet Travel Scheme and the EU Pet Movement Policy.
Epidemiol. Infect. doi:10.1017/S0950268809991221:1-12.
Thalwitzer, S., B. Wachter, N. Robert, G. Wibbelt, T. Muller, J. Lonzer, M. L. Meli, G. Bay, H. Hofer, and H. Lutz. 2009.
Seroprevalences to viral pathogens in free-ranging and captive cheetahs (Acinonyx jubatus) on Namibian farmland. Clin.
Vaccine Immunol. doi:10.1128/CVI.00345-09.
Annual report of OIE Reference Laboratories and Collaborating Centres, 2009
11
Zoonoses in Europe
Un, H., N. Johnson, A. Vos, T. Muller, A. R. Fooks, and O. Aylan. 2009. Genetic analysis of four human rabies cases reported
in Turkey between 2002 and 2006. Clinical Microbiology and Infection 15:1185-1189.
Vos, A., C. Freuling, S. Eskiizmirliler, H. Un, O. Aylan, N. Johnson, S. Gurbuz, W. Muller, N. Akkoca, T. Muller, A. R.
Fooks, and H. Askaroglu. 2009. Rabies in Foxes, Aegean Region, Turkey. Emerging Infectious Diseases 15:1620-1622.
Salmonellosis in cattle:
Berndt, A., J. Müller, L. Borsi, H. Kosmehl, U. Methner, and A. Berndt. 2009. Reorganisation of the caecal extracellular matrix
upon Salmonella infection--relation between bacterial invasiveness and expression of virulence genes. Vet. Microbiol.
133:123-137.
Methner, U., Heller, M., and Bocklisch, H. Salmonella enterica subspecies enterica serovar Choleraesuis in a wild boar
population in Germany. European Journal of Wildlife Research DOI 10.1007/s10344-009-0339-3. 2009.
TBE and other Tick-borne Diseases
Ticks and Tick-borne Diseases (TTBD) : an international, peer-reviewed scientific journal was founded in 2009.
The journal covers the following topics: Ticks; Ecology/ecoepidemiology of tick-borne diseases; Tick-borne
pathogens; Tick-borne diseases in domestic animals and wildlife; and Tick-borne human diseases: Editor-in-Chief:
Jochen Süss, FLI; Germany (www.TBD-SYMPOSIUM.COM):
Klaus, C., B. Hoffmann, U. Hering, B. Mielke, K. Sachse, M. Beer, and J. Süss. Clinical Microbiology and Infection, in press.
Süss, J. 2009. FSME-, RSSE-Virus, p. 886-891. In B. Neumeister (ed.), Mikrobiologische Diagnostik: Bakteriologie,
Mykologie, Virologie, Parasitologie. Thieme, Stuttgart.
Suss, J. 2009. Ticks - TBE - Climate change: do we have reliable data? Berliner und Munchener Tierarztliche Wochenschrift
122:481-482
Newcastle Disease: Häuslaigner, R., J. Sonnenburg, S. Kothlow, B. Kaspers, C. Staubach, and C. Grund. 2009. Evaluation
of an indirect enzyme-linked immunosorbent assay to study the specific humoral immune response of Muscovy ducks (Cairina
moschata) and domestic geese (Anser anser var. domestica) after vaccination against Newcastle disease virus. Avian Pathology
38:89-95
TSE: The FLI has made major contributions to the Manual of Diagnostic Tests and Vaccines for Terrestrial
Animals by the OIE that has been revised in 2008. The confirmatory methods applied for BSE and scrapie
diagnostics have been formulated in close contact with the FLI.
Beck, J., H. B. Urnovitz, M. H. Groschup, U. Ziegler, B. Brenig, and E. Schutz. 2009. Serum Nucleic Acids in an Experimental
Bovine Transmissible Spongiform Encephalopathy Model. Zoonoses and Public Health 56:384-390
Brenn, A., A. Karger, M. Skiba, U. Ziegler, and M. H. Groschup. 2009. A comprehensive proteome map of bovine
cerebrospinal fluid. Proteomics 9:5199-5205
Geissen, M., H. Mella, A. Saalmüller, M. Eiden, J. Proft, E. Pfaff, H. M. Schätzl, and M. H. Groschup. 2009. Inhibition of
prion amplification by expression of dominant inhibitory mutants--a systematic insertion mutagenesis study. Infect. Disord.
Drug Targets. 9:40-47
Gordon, P. M. K., E. Schutz, J. Beck, H. B. Urnovitz, C. Graham, R. Clark, S. Dudas, S. Czub, M. Sensen, B. Brenig, M. H.
Groschup, R. B. Church, and C. W. Sensen. 2009. Disease-specific motifs can be identified in circulating nucleic acids from
live elk and cattle infected with transmissible spongiform encephalopathies. Nucleic Acids Research 37:550-556
Kuczius, T., H. Karch, and M. H. Groschup. 2009. Differential solubility of prions is associated in manifold phenotypes.
Molecular and Cellular Neuroscience 42:226-233.
Polak, M. P., M. Larska, J. P. M. Langeveld, A. Buschmann, M. H. Groschup, and J. F. Zmudzinski. Veterinary Journal, in
press.
Vaccari, G., C. H. Panagiotidis, C. Acin, S. Peletto, F. Barillet, P. Acutis, A. Bossers, J. Langeveld, L. van Keulen, T.
Sklaviadis, J. J. Badiola, O. Andreoletti, M. H. Groschup, U. Agrimi, J. Foster, and W. Goldmann. 2009. State-of-the-art
review of goat TSE in the European Union, with special emphasis on PRNP genetics and epidemiology. Veterinary Research
40.
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Zoonoses in Europe
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