KAPI'OLANI COMMUNITY COLLEGE
University of Hawai'i
COURSE OUTLINE FORM (Form: 4/25/94)
MICRO 230 MOLECULAR BIOLOGY
1.
COURSE DESCRIPTION:
(10/27/2000)
MICRO 230 MOLECULAR BIOLOGY (3)
6 hours lecture/lab per week
Prerequisites:
MICRO 130 or MICRO 135 or BIOL 171;
MICRO 140 or MLT 107 or BIOL 171 L;
CHEM 151 or higher;
CHEM 151L or higher
This course covers the fundamental theoretical and
laboratory aspects of molecular biology. The basic
principles which govern the structure and function of
proteins, nucleic acids and macromolecular complexes
will be studied. Students will learn and become
proficient at performing the fundamental laboratory
procedures of biotechnology.
2.
COURSE OBJECTIVES/COMPETENCIES:
Upon successful completion of MICRO 230, the student
should be able to:
... Describe the structure of proteins, nucleic
acids and macromolecular complexes.
... Describe the function of nucleic acids,
proteins and macromolecules in DNA replication,
transcription, translation, mutagenesis and DNA
repair.
... Describe the regulation of gene activity in
prokaryotes and eukaryotes.
... Describe basic principles and techniques of
molecular biology including the use of plasmids
and transposons to generate recombinant DNA.
1
... Prepare, sterilize and dispense the basic
types of media used for the cultivation of
bacteria.
... Operate all the basic equipment of a
molecular biology laboratory, including but not
limited to large autoclaves and bench top
autoclaves, water distillation apparatus,
biological safety cabinets, spectrophotometers and
ELISA readers, electrophoresis equipment,
centrifuges and microcentrifuges.
...
Perform agarose gel electrophoresis
... Isolate and quantitate chromosomal and
plasmid DNA from bacteria
... Perform and analyze restriction enzyme
digestions of DNA
...
Prepare and screen a genomic library.
... Prepare enzyme labeled probes and perform
southern blots.
... Perform polymerase chain reactions under a
variety of conditions.
... Analyze DNA and amino acid sequence data by
searching sequence data bases.
3.
GENERAL EDUCATION AND RELATIONSHIP TO OTHER COURSES:
MICRO 230 is an additional elective course in the MLT
curriculum. This course adds a molecular biology
component to the curriculum and is intended to help
prepare MLT students for work in biotechnology
laboratories. MICRO 230 also meets requirements as a
Group 1 Biological Science (NS1) elective in the
Liberal Arts curriculum. This is a sophomore level
biological science laboratory course and as such it
requires a significant foundation with laboratory
experience in freshman biology and chemistry. Hence
the prerequisites of MICRO 130 or MICRO 135 or BIOL
171; and MICRO 140 or MLT 107 or BIOL 171L; and CHEM
151 or higher; and CHEM 151L or higher.
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This course supports the following college competency
areas:
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computation and communication abilities
values for living
quality of life as affected by technology and
awareness of the dynamics in contemporary issues
problem-solving and decision-making abilities
responsiveness to the arts and humanities
career choices and life-long learning
study in a selected program
science
This course also satisfies the following Associate in
Science degree competencies:
...
Employ skills and understandings in language and
mathematics essential to fulfill program
requirements.
... Understand attitudes and values of various
cultures and examine their potential for improving the
quality of life and meaningfulness in work.
...
Recognize efforts of technology and science on the
natural and human environments.
...
Understand contemporary issues and problems and
respond to the impact of current conditions.
...
Demonstrate abilities of conceptual, analytic, and
critical modes of thinking.
... Develop insights in human experience and apply
them
to personal, occupational, and social
relationships.
...
Recognize relevance of career choices to life-long
learning.
...
Demonstrate competence in a selected program of
study.
This course satisfies the following program
competencies:
... Demonstrate technical skills, social behavior and
professional awareness incumbent upon a laboratory
technician as defined by the American Society for
Clinical Laboratory Science (ASCLS) and the American
Society of Clinical Pathologists (ASCP).
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... Apply systemized problem solving techniques to
identify and correct procedural errors, identify
instrument malfunctions and seek proper supervisory
assistance and verify the accuracy of laboratory
results obtained.
...Operate and maintain laboratory equpment, utilizing
appropriate quality control procedures.
This course also satisfies the following Associate in
Arts degree competencies:
... Employ those skills in communication, mathematics,
and historical content essential to further college
work.
... Follow those steps employed in the scientific
method for valid conclusions or demonstrable hypotheses
as used in the natural sciences.
... Correlate the skills and understanding learned in
the physical and biological sciences to produce an
awareness
of our technological and natural
environment.
... Gather and filter data, compose and refine
conclusions, solutions, and alternatives to issues or
concerns posed
in science courses.
... Show, by completion of elective and/or required
courses, the educational background necessary for more
specific professional and personal goals.
... Make a decision if desired about further course of
study in a four-year college, with a capacity to
declare a major and select courses directed toward that
major, based upon a realistic assessment of personal
needs and aspirations.
This course also satisfies the following Associate in
Arts degree requirements:
This course fulfills the requirements as a Group I
Natural Science for both the KCC AA Degree and the
UHM Core.
This course also satisfies the following Associate in
Science degree requirements:
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This course fulfills the requirements as a Natural
Science for both the KCC AS Degree and the UHM
Core.
MICRO 230 satisfies the following departmental and/or
program competencies:
... Demonstrate awareness of science as a discipline
that has been and continues to be objective, rational,
cumulative, international, and quantitative.
... Reason mathematically and understand mathematical
concepts.
... Apply mathematical reasoning and concepts in a
study of the relationship of mathematics to the modern
world.
... Express the ethical nature of the scientific
attitude
with attention to its strict intellectual honesty and
accuracy of observation, calculation, and conclusion.
... Show an awareness of the fundamental physical,
chemical, and biological processes that operate in the
human individual and in the human environment.
... Understand the observational and experimental
techniques and methodologies employed in the natural
sciences.
... Make simple measurements and test of reality
according to a degree relative to current and future
lifestyles.
... Show acquisition of sufficient scientific
knowledge
necessary to continue pursuit of anticipated academic,
vocational and personal needs.
4.
COURSE CONTENT:
Week
1
Topic
Lecture:
Introduction
Quick review of Mendelian
genetics
Lab:
5
Basic Equipment and Techniques
Review of bacteriological
techniques
Preparation of media and
reagents
2
Lecture:
Molecular Genetics
DNA structure
Replication
Genes and genetic elements
Mechanisms of recombination
transformation, conjugation,
transduction, transposition
Lab:
Basic Equipment and Techniques
Review of bacteriological
techniques
Preparation of media and
reagents
Isolation of luminescent
bacteria from natural sources
3
4
Lecture:
Molecular Genetics continued
DNA structure
Replication
Genes and genetic elements
Mechanisms of recombination
transformation, conjugation,
transduction, transposition.
Lab:
Agarose gel electrophoresis
Restriction digestion of
plasmid DNA
Lecture:
Molecular Genetics continued
RNA genes and RNA structure
The genetic code
Protein synthesis
Lab:
Isolation of chromosomal DNA
from bacteria
Purification of plasmid DNA
5
Lecture:
Restriction enzymes
6
Digestion, recombination and
ligation
6
7
8
9
10
11
Lab:
Spectrophotometric analysis of
DNA
Quantitation of genomic DNA
Restriction digestion of
genomic DNA
Lecture:
Hosts and vectors
Lab:
Ligation of restriction
fragments to a plasmid
vector
Preparation of competent cells
Transformation of competent
cells with
recombinant
plasmids
Lecture: Genomic, cDNA and
expression Libraries
Lab:
Screening of the genomic
library
Lecture:
Sequencing and interpreting
sequences
Lab:
Isolation of plasmids
containing the gene of interest
Lecture:
Gene regulation - mechanisms of
differential gene expression
Lab:
Restriction mapping isolated
plasmids
Lecture:
Gene regulation - mechanisms of
differential gene expression
Lab:
Southern blotting and RFLP
Purification and subcloning
Colony hybridization to screen
subclones
Lecture:
Molecular biology of cancer
7
12
13
14
15
Lab:
Polymerase chain reaction
Lecture:
Human genetic applications
Lab:
DNA sequencing
Lecture:
Human genetic applications
Lab:
Computer analysis of genetic
sequences
Lecture:
Applications in medicine,
agriculture and industry
Lab:
Advanced techniques
Lecture:
Applications in medicine,
agriculture and industry
Lab:
Loose ends and closure
5.
POSSIBLE TEXTS:
Winfrey, M.R., Rott, M.A., and Wortman, A.T.,
Unraveling DNA: Molecular Biology for the Laboratory,
1997, Prentice-Hall, Inc.
Bloom, M.V., Freyer, G.A., and Micklos, D.A.,
Laboratory DNA Science, 1996, The Benjamin/Cummings
Publishing Company, Inc.
Weaver, Robert F.,Molecular Biology, 1999, WCB/McGrawHill.
Malacinski, G.M. and Freifelder, D., Essentials of
Molecular Biology, 3rd Edition, 1998, Jones and
Bartlett Publishers, Inc.
Students will be expected to purchase a lab coat,
latex/vinyl gloves and safety glasses for this course..
6.
REFERENCE MATERIALS:
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Additional resources, such as textbooks and journals,
are available in the library, the MLT laboratory the
microbiology laboratory and the Natural Science
Learning Assistance Center. Web resources will also be
used in this course and they can be accessed from
numerous computers on campus or from personal computers
off campus.
7.
AUXILIARY MATERIALS AND CONTENT:
Videotapes, CD-ROM and other computer-assisted
instructional material may be used.
8.
METHODS OF INSTRUCTION:
Lecture, discussion and laboratory experiments will be
used.
9.
METHOD OF EVALUATION:
2 midterm exams at 50 points each.
5 laboratory reports at 20 points each.
10 quizzes at 10 points each.
Total = 300 points
90% - 100% = A
80% - 89% = B
70% - 79% = C
60% - 69% = D
Less than 60% = F
10.
JUSTIFICATION:
A.
This course adds a new dimension to the MLT
curriculum and will help to expand the job
opportunities in biotechnology for graduates of
the program. The course also broadens the scope
and nature of the biological science course
offerings at KCC and is essential to the
development of a biotechnology emphasis at this
institution. Also, the Hawai’i Millenium Workforce
Development Initiative has identified
biotechnology as a priority area for development
and has awarded KCC a grant to develop a
biotechnology program. This course would be an
essential component of such a biotechnology
program.
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B.
This course is being taught on an
experimental basis this semester (Fall 2000).
There are 12 students enrolled and most of these
are medical technologists or medical laboratory
technicians who are taking this course because
they see molecular biology as increasingly
important to their work. This course is currently
the only one of its kind offered in the State.
Furthermore, there are two college faculty members
who are sitting in on the course in order to
strengthen their mastery of the subject.
C.
This course will not increase or decrease the
number of required hours needed for a certificate
or degree.
11.
RESOURCE REQUIREMENTS:
A.
Source of funding -- We were awarded $35,251
by the Hawai’i Millenium Workforce Development
Initiative for the initial planning phase of the
biotechnology program during the 99/00 academic
year. Approximately $15,000 of that was used for
the purchase of the supplies and equipment needed
to set up the laboratory facilities for this
course.
B.
This course will not have an impact on other
departments in the areas of prerequisites or
program support. The laboratory for this
course will be conducted in the microbiology
laboratory, Kokio 106. This course will
impose a significant time requirement on the
facility since students will have to also
work on experiments outside of the regularly
scheduled class time. Currently there are
blocks of time available on Tuesdays,
Thursdays and Fridays which could accommodate
those requirements.
This course will be taught by the faculty and
lecturers of the Math/Science Department. It
is a new course and it does not replace any
offerings of the Department; as such it
represents an expansion of the Department’s
offerings. Once the start-up funding is
expended, the cost of the course will be
covered by the Department’s regular
allotment.
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C.
Maximum enrollment in this course is 20
students.
12.
ARTICULATION:
A.
There is no similar course in the University
of Hawai’i system.
B.
This course is appropriate for articulation
and acceptance into the core for UHM.
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