Holocene Rock Varnish Microstratigraphy and its Chronometric Application in the Drylands of western USA Tanzhuo Liu and Wallace S. Broecker Lamont-Doherty Earth Observatory, Columbia University, Palisades, New York, NY 10964, USA Abstract Analyses of hundreds of rock varnish samples from latest Pleistocene and Holocene geomorphic features in the drylands of western USA reveal a regionally replicable Holocene layering sequence. This sequence consists of twelve approximately evenly-spaced weak dark layers interdigitated with thirteen orange/yellow layers. Preliminary radiometric age calibration indicates that six dark layers in the upper portion of the sequence deposited during the last 6000 years, diagnostic of the Little Ice Age and late Holocene wet events; five dark layers in the lower portion of the sequence deposited after the termination of the Pleistocene but slightly before 7000 years BP, indicative of the early Holocene wet events; one dark layer in the middle portion of the sequence deposited around 6500 years BP, suggestive of the middle Holocene wet phase. Our age calibration further indicates that the Holocene wet events represented by those dark layers largely correlate in time with the millennial-scale Holocene cooling events in the North Atlantic region (Bond et a., 1997, 1999). This radiometrically calibrated and climatically correlated Holocene varnish layering sequence was then used as a unique correlative dating tool to determine the surface exposure ages of geomorphic and geoarchaeological features in the western US deserts. The varnish layering dating of debris-flow fan deposits in Death Valley, California yields minimum ages of 12 500, 12 500-11 100, 11 100, 10 300, 9400, and 2800 years BP for six debris-flow fan building events, suggesting that such events are more likely to have occurred during relatively wet periods of the Holocene. The varnish layering dating of a historic grinding stone from Chili of northern New Mexico yields a minimum age of 900-1100 years BP for the abandonment of this occupation site by the Anasazi Indians. The varnish layering dating of a prehistoric flaked stone (i.e., a primary core) from Ocotillo, southern California yields a minimum age of 12 500 years BP for the making of this stone artifact, suggesting at least a Paleo-Indian human occupation at Ocotillo during the terminal Pleistocene. These results indicate that, when properly applied, varnish layering dating has the great potential to yield numerical age assignments for surface stone tools, petroglyphs, and geoglyphs of both historic and prehistoric age in the drylands of western USA. Keywords: Rock varnish dating; Wet events; Holocene climate; Debris-flow fan; Petroglyph; Stone artifact; The Great Basin 2 1. Introduction Rock varnish is a dark to dark-brown coating on subaerially exposed rock surfaces. It is probably the world's slowest-accumulating sedimentary deposit, growing at only a few to tens of microns per thousand years (Liu and Broecker, 2000). Its thickness ranges from less than 5 up to 600 µm, with a typical thickness of 100 µm or so. Although found in all terrestrial environments, rock varnish is mostly developed and well preserved in arid to semiarid deserts of the world. It is composed of about 30% manganese and iron oxides, up to 70% clay minerals, and over a dozen trace and rare earth elements (Potter and Rossman, 1977, 1979). The building blocks of rock varnish are largely blown in as airborne dust (Fleisher et al., 1999; Moore et al., 2001; Thiagarajan and Lee, 2004). Microlaminations in rock varnish were first reported by Perry and Adams (1978), who recognized their potential as a paleoenvironmental indicator in drylands. Microlaminations can be observed when the varnish is shaved thin enough (5-10 µm) to see through in ultra-thin section with a light microscope (Fig. 1). Electron microprobe chemical mapping reveals that dark layers in varnish thin section are rich in Mn and Ba, but poor in Si and Al, while orange and yellow layers are poor in Mn and Ba, but rich in Si and Al (Fig. 1). These two types of layers are intercalated to form distinct microstratigraphy in varnish. A growing body of evidence indicates that varnish microstratigraphy carries a climatic record (Dorn, 1984; Dorn, 1990; Cremaschi, 1996; Liu and Dorn, 1996; Liu et al., 2000; Broecker and Liu, 2001). In the Great Basin of western USA, Mn-poor yellow layers (usually containing 5-15% MnO) formed during dry periods of the Holocene and the last interglacial, while Mn-rich black layers (usually containing 25-45% MnO) 3 deposited during wet periods of the last glacial time; Mn-intermediate orange layers (usually containing 15-25% MnO) formed during periods of climatic transition between extremely dry and extremely wet condition (Broecker and Liu, 2001). Furthermore, the glacial-age black layers in varnish microstratigraphy appear to correlate in time with the cold episodes of the Younger Dryas and Heinrich Events (Broecker, 1994; Bond et al., 1993) in the North Atlantic region (Liu and Dorn, 1996; Liu et al., 2000; Liu, 2003). Varnish microstratigraphy as a correlative dating technique (cf. terminology in Colman et al., 1987) is relatively new and different in principle and independent from both cation-ratio and AMS 14C methods (Dorn, 1983; Dorn et al., 1989). It was first used by Dorn (1988) to study the chronostratigraphy of alluvial-fan deposits in Death Valley of California. Subsequent studies by others (Liu, 1994; Liu and Dorn, 1996; Liu, 2003) have greatly improved the usability of the technique. The basic assumption of this dating approach is that the formation of varnish microstratigraphy is largely influenced by regional climatic variations. Since climatic signals recorded in varnish have proven to be regionally contemporaneous (Liu and Dorn, 1996; Liu et al., 2000), varnish microstratigraphy has the potential use as a tephrachronology-like dating tool. Recently a rigorous and completely blind test of this method has been conducted on late Quaternary lava flows in the Mojave Desert, California (Liu, 2003; Phillips, 2003). The testing results demonstrate that varnish microstratigraphy is a valid dating tool to provide surface exposure age for late Pleistocene surficial geomorphic features in the Great Basin of western USA (Marston, 2003). The purpose of this paper is to extend the utility of varnish microstratigraphy method to surficial geomorphic and geoarchaeological features of the Holocene. For this 4 purpose, we need to: (1) systematically establish the Holocene varnish microstratigraphy for the Great Basin of western USA; (2) radiometrically calibrate microlaminations in the varnish microstratigraphy; and (3) demonstrate the potential use of this dating technique in determination of surface exposure age for various geomorphic and geoarchaeological features in the drylands of western USA. 2. Study region and methods The Great Basin and its vicinities of western USA have been chosen as our study region for several reasons (Fig. 2). First, previous studies (Liu, 1994; Liu and Dorn, 1996; Liu, 2003) have established and radiometrically calibrated the varnish microstratigraphy of late Pleistocene age for the region, providing a temporal framework for further studying of the Holocene varnish microstratigraphy. Second, fine-grained and fastgrowing rock varnish of latest Pleistocene to late Holocene age is well developed in the study region, facilitating generalization of high-resolution Holocene varnish microstratigraphy. Third, the study region also hosts numerous latest Pleistocene and Holocene geomorphic features that have been dated by radiometric methods (Table 1), thus providing opportunities for radiometric calibration of Holocene varnish microstratigraphy. In order to establish a complete Holocene varnish microstratigraphy for the study region, varnish samples were intentionally collected from well-dated geomorphic features of latest Pleistocene age (Fig. 2; Table 1). These features include the highstand shorelines of Summer Lake (~ 18 ka; see Fig. 3F of this study; Freidel, 1993), Bonneville Lake (~ 14.4 14C ka; Cerling and Craig, 1994), Lahontan Lake (~ 13.6 14C ka; Benson et al., 5 1995), Searles Lake (~ 14 14C ka; Benson et al., 1990), Silver Lake (10.5-15 14C ka; Wells et al., 1987), Panamint Lake (~ 15 14C ka; Jayko et al., 2001); and the Owens River Dry Falls in Owens Valley (~ 15.9 3He ka; Cerling, 1990). For some localities in central Arizona (i.e., McDowell Mountain) and southern California (i.e., Blythe) where such well-dated geomorphic features don’t exist, samples were collected from alluvial-fan surfaces of latest Pleistocene age based on field observation and judgment (Fig. 2). To radiometrically calibrate Holocene varnish microstratigraphy, samples were collected from well-dated geomorphic features of Holocene age in the study region (Fig. 2; Table 3). These features include radiocarbon dated alluvial-fan surfaces in Las Vegas Valley of southern Nevada (~ 9.9 14C ka; Bell et al, 1998a), Fish Lake Valley of southern Nevada (0.5-6.5 14C ka; Reheis et al., 1993, 1995), Silurian Valley of southern California (~ 1.8 14C ka; Anderson and Wells, 2003), Ajo Mountains of southern Arizona (~ 2.5 14C ka; Pohl, 1995), cosmogenic 3He dated debris flow boulders in the Grand Canyon of northern Arizona (~ 1.5 ka; Cerling, 1997, written comm.), cosmogenic 36Cl dated Carrizozo lava flows (~ 5.2 ka; Dunbar, 2002), and radiocarbon dated McCartys lava flows (~ 3.0 14C ka; Laughlin et al., 1994) in central New Mexico. To demonstrate the potential use of varnish microstratigraphy method as a unique geomorphic and geoarchaeological dating tool in the drylands of western USA, varnish samples were collected from varied age surfaces of a debris-flow fan in Death Valley of southern California; a grinding stone (i.e., metate) near Chili of northern New Mexico, and a stone artifact (i.e., a primary core) near Ocotillo of southern California (Fig. 2; Table 2). 6 A key to this study is an innovative method of making ultra thin sections of rock varnish (Liu, 1994; Liu and Dorn, 1996), which reduces failure rates from 80% to <5% and permits rapid preparation and hence inter-comparison of many sections. The conventional way of making varnish thin sections cannot be employed because the resulting thin sections are too thick (25-30 µm) and do not reveal the microstratigraphy, which is opaque in normal geological thin sections. Ultra-thin sections (5-10 µm) of varnish, in contrast, allow for identification and analysis of late Pleistocene varnish microstratigraphy (see Fig.1). Unlike pre-Holocene varnish microstratigraphy, however, ultra-thin sections need to be made slightly thicker (10-15 µm) to see Holocene varnish microstratigraphy. Otherwise, the entire Holocene microstratigraphy would be turned into a single surface yellow layer in ultra-thin section (see the inset image of optical varnish microstratigraphy in Fig. 1). Varnish ultra-thin sections were then photographed on Kodachrome ASA 64 film using a Leica DMLB light microscope, equipped with a Leica MPS 60 Photoautomat system. A polarized light was utilized to increase slide contrast. The use of 10 eyepiece in combination with 40 objective lens yields good quality of color slides with 400 magnification. Kodak Royal Gold ASA 100 color print films were used to make prints of the color slides on a slide duplicator. Both slide and color print films were commercially developed after exposure. Color slides and prints thus obtained provide high-resolution (~ 1 µm) images of optical varnish microstratigraphy for layering pattern analysis. Varnish ultra-thin sections which contain clearly-defined microstratigraphies were selected for further electron microprobe chemical analyses. X-ray elemental mapping and 7 line profile were obtained on a fully-automated five-spectrometer CAMECA SX100 electron probe, with the use of quantitative mode of wavelength dispersive x-ray spectrometry (WDS). Probe mapping provides high-resolution (~ 2 µm) images of chemical varnish microstratigraphy. Combination of both optical and chemical microstratigraphy allows detailed detection of Mn, Ba, and other elemental fluctuations in varnish. In this study, we collected 138 varnish samples from 20 different localities that cover most parts of the Great Basin and its vicinities in the western US drylands. A total of 414 ultra-thin sections were made and 1078 spot chemical measurements were obtained by electron microprobe along 25 individual line profiles. These chemical data, together with 754 high-resolution (~ 1 µm) optical images of varnish microstratigraphy, form the database used to generalize and correlate varnish layering sequences. 3. Results 3.1. Holocene varnish microstratigraphy Our results indicate that, by carefully selecting fine-grained (< 0.1 µm in size) and fast-growing (10-20 µm/ky) varnish and by making slightly thicker (10-15 µm) varnish ultra-thin sections, we can detect and assess relatively weak signals of Holocene wet phases in the western US drylands. For instance, a fast-growing varnish of Holocene age from the ~ 16 800 yr BP (in calendar years before present; Table 1) highstand shorelines of Searles Lake in the Mojave Desert displays eleven approximately evenly-spaced weak black layers (Fig. 3A). Similar layering sequences were observed in varnish samples from latest Pleistocene highstand shorelines of Summer Lake (OR), Lahontan Lake (NV), and 8 Panamint Lake (CA) and from the Owens River Dry Falls (CA) in the study region (Figs. 3B,D-F). One varnish sample from an alluvial-fan surface of latest Pleistocene age in the McDowell Mountain of Arizona displays the most complete Holocene layering sequence (Fig. 3C). Based on examination of hundreds of varnish microstratigraphies from the study region, we established a generalized Holocene layering sequence in varnish (Fig. 4). This sequence contains twelve approximately evenly-spaced Mn- and Ba-rich weak black layers interdigitated with thirteen Mn- and Ba-poor orange/yellow layers. Six of these black layers, called here as WH1 to WH6 (WH stands for “Wet event in Holocene”), occur in the upper portion of the sequence, likely indicative of the Little Ice Age and late Holocene wet phases in the study region. Five of these black layers WH8 to WH12 appear in the lower portion of the sequence, probably indicative of early Holocene wet phases. There is one relatively weak dark layer WH7 roughly in the middle portion of the sequence, likely indicative of a middle Holocene weak wet phase. Other orange/yellow layers in the sequence appear to be diagnostic of Holocene dry phases in the study region. Table 2 presents a detailed description of characteristics of these black and orange/yellow layers in the Holocene varnish layering sequence. Figure 5 shows comparison between optical and chemical microstratigraphies in varnish samples from three locales along an N-S traverse in southern California. It can be seen that, for a given varnish microstratigraphy, chemical signals of Mn and Ba in the Holocene portion of the layering sequence are generally much weaker than those in the pre-Holocene sequence. Compared to the pre-Holocene black layers that contain 25-45% MnO and 2-6% BaO, the Holocene black layers often contain only about 15-25% MnO 9 and less than 1-4% BaO (Fig. 5). This observation is consistent with the fact that the overall climate during the last glacial was much wetter than during the current interglacial in the drylands of western USA (Broecker and Liu, 2000). Moreover, black layers WH1 to WH12 observed in the optical microstratigraphy can be clearly identified as minor Mnand Ba-rich peaks in the chemical microstratigraphy (Fig. 5). Also, as seen in Fig. 5, there are regional variations of Mn and Ba content in the Holocene varnish layers along the traverse. On average, the Holocene layers in varnish from Death Valley contains <10% MnO and <0.5% BaO, while those from Blythe contains about 10% MnO and 0.5-1.0% BaO, and those from Ocotillo contains about >10-15% MnO and >1.0-1.5% BaO. Such variations of Mn and Ba content in the Holocene varnish layers seem to reflect the present-day effective moisture gradient along the traverse: from the most arid land with less summer monsoonal precipitation in Death Valley of the Mojave Desert to a relatively less arid region with more summer monsoonal precipitation in Ocotillo of the Yuma Desert. Figure 6 presents chemical microstratigraphies of Ba for varnish samples of different age and inter-sample correlation of Holocene dark layers WH1-WH12 represented by Ba peaks. We found that the chemical signals of Ba in the lower portion of the Holocene microstratigraphy are generally stronger than those in the upper portion (Figs. 6A,B), likely indicative of an overall wetter climate condition during the early Holocene than during the late Holocene in the study region. We also noticed that the Ba peaks of dark layers WH1-WH6 in varnish of late Holocene age are easily recognized and correlated in the chemical microstratigraphy (Figs. 6C-E), and so are the Ba peaks of dark layers WH8-WH12 in varnish of early Holocene age (Figs. 6A,B). Due to differential 10 varnish growth, however, the Ba peaks of dark layer WH1-WH6 in early Holocene varnish are often more likely to be “squeezed up” than those in late Holocene varnish, which may cause some ambiguity in inter-sample correlation of these dark layers (Figs. 6A,C). In most cases, the use of both optical and chemical microstratigraphies (see Fig. 5) helps reduce such ambiguity and assures correct identification and inter-sample correlation of the Holocene dark layers. 3.2. Climatic correlation The Holocene wetness variations uncovered in varnish microstratigraphy of the western US drylands appear to have a striking correlation with the Holocene climate events in the North Atlantic region (Fig. 4). Bond et al. (1997, 1999) discovered a pervasive millennial-scale cycle, with a cyclicity of 1470 ± 500 years, of Holocene events and abrupt climate shifts during the last glaciation in the North Atlantic deep sea cores. The discovery most interesting to this study is that the Holocene portion of the climate record revealed a total of nine cooling events, with the Little Ice Age (LIA) being the most recent cold phase in the series of millennial-scale cycles. For the sake of simplicity, Bond et al. (1997, 1999) named these events (from oldest to youngest) as 8, 7, 6, 5, 4, 3, 2, 1 and 0 cooling events, and their reported ages are ca. 11 100, 10 300, 9400, 8100, 5900, 4100, 2800, 1400, and 650-300 yr BP, respectively (Fig. 4). Several lines of evidence support a possible correlation of the varnish climate record with the deep sea record. First, our previous studies in the Great Basin of western USA have shown that the formation of pre-Holocene dark layers in varnish microstratigraphy was contemporaneous with the abrupt climate shifts such as Heinrich 11 events during the last glaciation (Liu and Dorn, 1996; Liu et al., 2000; Broecker and Liu, 2000; Liu, 2003). In the Las Vegas Valley of southern Nevada, black layer WP0 (WP stands for “Wet event in Pleistocene”; also see Liu, 2003 for more discussion on this topic) in the latest Pleistocene varnish microstratigraphy has proven to correlate in time with the Younger Dryas (YD) cold snap in the North Atlantic (Liu et al., 2000). The YD cooling was considered as one of those abrupt climate shifts that manifest the millennialscale climatic cyclicity (Bond et al., 1997, 1999). Second, there is nearly a perfect match in the number of wet/cold phases between the two climate records (Fig. 4). Our current study has documented a total of 12 black layers in varnish microstratigraphy, each representing a Holocene wet phase in the western US drylands. Four black layers of the early Holocene, namely WH12, WH11, WH10 and WH9, presumably match four early Holocene cooling events 8, 7, 6, 5; and four late Holocene black layers WH6, WH5, WH4, WH3 presumably match four late Holocene cooling events 4, 3, 2, 1. Since the topmost black layer WH1 in the varnish microstratigraphy represents the most recent wet phase during the Holocene, it most likely match the LIA cooling. Although black layers WH8, WH7, and WH2 each have no clear match to any of the nine cooling events reported by Bond et al. (1997, 1999), further examination of the deep sea record suggests that these black layers may match some minor cold peaks in the record, as indicated by a question mark (?) in Fig. 4. Specifically, if such climatic correlation exists, the deposition of black layers WH8, WH7, WH2 in the varnish microstratigraphy likely occurred around 7300, 6500, 1100-900 yr BP, respectively, based on the chronology of the deep sea record (Bond et al., 1997, 1999; Fig. 4). 12 Third, there is a close tie-up in the cyclicity of the Holocene wet/cold events in both records. Given a well-known duration of ~ 12 000 years for the Holocene, a total of 12 roughly evenly-spaced black layers in the Holocene varnish microstratigraphy implicate, to the first degree of approximation, a 1000-year cyclicity of wet events in the drylands of western USA. Such cyclicity overlaps, within ± 1 error, the reported cyclicity of 1374 ± 502 years for the Holocene cooling events, or 1470 ± 500 years for both the Holocene and the last glacial cold events in the North Atlantic region. In summary, although still lack of a detailed radiometric chronology at this moment, the evidence presented above seems to support a tentative correlation between the Holocene varnish climate record in the western US drylands and the Holocene deep sea record in the North Atlantic. 3.3. Age calibration In order to make a firm correlation between the varnish climate record and the deep sea record, we need to build a reliable radiometric chronology for the Holocene varnish microstratigraphy. Unlike the pre-Holocene layering sequence that has been radiometrically calibrated (Liu, 2003), it is extremely difficult to calibrate the Holocene sequence for the following reasons. First, the Holocene sequence contains a total of 25 black and orange/yellow layers within a time period of ~ 12 000 years. This suggests an average duration of nearly 500 years for each individual varnish layer and thus requires much more accurate and precise radiometric age constraints for calibration. Secondly, although it would be ideal to date each individual layers within varnish microstratigraphy, such breakthrough has not yet been accomplished. So our approach to dating varnish 13 layers was still indirect: examining samples from sites with radiometric age control for the underlying landform. Such age control is maximum for the start of the layering sequence but minimum for the age of the geomorphic feature, because the varnish started to form only after the dated landform was created. Lastly, there are only few localities in the western US drylands where varnished Holocene geomorphic features have been accurately and precisely dated by radiometric means to provide unambiguous age constraints in the study region (Table 1). However, the results of our preliminary age calibration for the Holocene varnish microstratigraphy are very promising (Figs. 7 and 8). Most of our calibration samples were from radiocarbon dated Holocene alluvial-fan surfaces (500-7500 yr BP; Table 3) in Indian Creek, Leidy Creek, and Trail Canyon of Fish Lake Valley, Nevada (Reheis et al., 1993, 1995). Varnish from the youngest radiocarbon dated alluvial-fan surface (540 ± 30 yr BP; Table 3) in Trail Canyon displays a latest Holocene layering sequence LU-1 (WH1) (Fig. 7A), which stands for “Layering Unit 1 with a basal layer WH1” (see Liu, 2003 for terminology). If the lag time of varnish initiation on subaerially exposed rock surfaces is assumed to be ~ 200 years (see discussion on this topic in Liu, 2003), the basal dark layer WH1 in the varnish probably deposited around 300-400 yr BP, which is synchronous with the LIA cold snap that culminated 300 years ago (Fig. 8). Varnish from radiocarbon dated alluvial-fan surfaces in Leidy Creek of Fish Lake Valley (1510 ± 70 yr BP) and Silurian Valley of the Mojave Desert (1700 ± 70 yr BP) displays two similar and well-defined late Holocene layering sequences LU-1 (WH3) (Figs. 7D,E; Table 3). If the varnish lag time of ~ 200 years is still assumed, the deposition of the basal dark layer WH3 likely occurred around 1300-1500 yr BP, which largely correlates in time with the 14 late Holocene cooling event 1 (ca. 1400 yr BP) in the North Atlantic region (Fig. 8). Varnish from radiocarbon dated alluvial-fan surfaces (4280 ± 150 yr BP) in Leidy Creek displays a late Pleistocene layering sequence LU-1 (WH5) (Fig. 7I). Given a varnish lag time of ~ 200 years, the deposition of the basal dark layer WH5 likely occurred around 4000-4100 yr BP, which perfectly correlates in time with the late Holocene cooling event 3 (ca. 4100 yr BP) in the North Atlantic region (Fig. 8). Varnish from the Carrizozo lava flows in central New Mexico displays a late Holocene layering sequence LU-1 (WH6) (Fig. 7K). Dunbar (2002) reported three cosmogenic 36Cl ages for emplacement of the flows: 4900 ± 500, 5400 ± 1000, and 5600 ± 900 yr BP, with a weighted average of 5200 ± 700 yr BP. If the maximum flow age of 5600 ± 900 yr BP is assumed, the basal dark layer WH6 in the varnish from the flows probably deposited around a time that is largely close, within ± 1 error, to ca. 5900 yr BP as implicated by WH6 (Fig. 8). Further, varnish from radiocarbon dated alluvial-fan surfaces (11421 ± 458 yr BP) in Las Vegas Valley of southern Nevada (Liu et al., 2000) displays a nearly complete Holocene layering sequence LU-1 (WH12) (Fig. 7N). Given a ~ 200 years of varnish lag time, the basal dark layer WH12 in the varnish likely formed around 11 200-11 300 yr BP, a time that is in accord with the reported age of ca.11 100 yr BP for the earliest Holocene cooling event 8 in the North Atlantic (Fig. 8 ). For dark layers WH2, WH7, and WH8 in the Holocene layering sequence, the results of our preliminary age calibration are also very encouraging. One varnish sample from radiocarbon dated latest Holocene alluvial-fan surfaces (1320 ± 190 yr BP) in Trail Canyon displays a latest Holocene layering sequence LU-1 (WH2) (Fig. 7B). Given a varnish lag time of ~ 200 years, the basal dark layer WH2 likely deposited around 110015 1200 yr BP, which is fairly consistent with the correlative age of ca. 900-1100 yr BP for WH2 (Fig. 8). Another varnish sample from radiocarbon dated early Holocene alluvialfan surfaces (7450 ± 80 yr BP) in Indian Creek displays a clearly-defined layering sequence LU-1 (WH8) (Fig. 7M). Given a varnish lag time of ~ 200 years, the basal dark layer WH8 likely deposited around 7200-7300 yr BP, which perfectly matches the correlative age of ca. 7300 yr BP for WH8 (Fig. 8). In this study, no age control was obtained for WH7, a relatively weak dark layer at the middle point of the Holocene layering sequence. However, one varnish sample from radiocarbon dated middle Holocene alluvial-fan surfaces (7181 ± 120 yr BP) in Trail Canyon displays a clearlydefined layering sequence LU-1 (WH7+) (Fig. 7L); here WH7+ stands for the orange layer directly underneath dark layer WH7 (Fig. 8). Given a varnish lag time of ~ 200 years, the basal orange layer WH7+ likely deposited around 6900-7000 yr BP, thus predating dark layer WH7 that is presumably at ca. 6500 yr BP. For those orange/yellow layers in the Holocene layering sequence, our preliminary age calibration provides reasonable time constraints. Varnish from cosmogenic 3He dated ~ 1500 yr BP debris-flow boulders in the Grand Canyon of northern Arizona (Cerling, 1997, written comm.) displays a latest Holocene layering sequence LU-1 (WH2+) (Fig. 7C). Given a lag time of ~ 200 years for the varnish initiation, this layering sequence suggests an age of 1300 yr BP for the basal orange layer WH2+, which predates dark layer WH1 at ca. 900-1100 yr BP and postdates dark layer WH3 at ca. 1400 yr BP (Fig. 8). Varnish from radiocarbon dated alluvial terraces (2613 ± 110 yr BP) in the Ajo Mountains of southern Arizona (Pohl, 1995) displays a late Holocene layering sequence LU-1 (WH3+) (Fig. 7F). Given a lag time of ~ 200 years for the varnish initiation, this 16 layering sequence suggests an age of 2400-2500 yr BP for the basal orange layer WH3+, which postdates dark layer WH4 at ca. 2800 yr BP (Fig. 8). Similarly, varnish from radiocarbon dated McCartys lava flows (3170 ± 140 yr BP) in central New Mexico (Laughlin et al., 1994) and alluvial-fan surfaces (3630 ± 80 yr BP) in Leidy Creek of Fish Lake Valley displays a late Holocene layering sequence LU-1 (WH4+) (Figs. 7G,H). This suggests an age of 3000-3400 yr BP for the basal orange layer WH4+ that predates dark layer WH4 at ca. 2800 yr BP and postdates dark layer WH5 at ca. 4100 yr BP (Fig. 8). Furthermore, varnish from radiocarbon dated alluvial-fan surfaces in Leidy Creek (5750 ± 90 yr BP) displays a well-defined late Holocene layering sequence LU-1 (WH5+) (Fig. 7J), suggesting an age of 5500-5600 yr BP for the basal orange layer WH5+ that predates dark layer WH5 at ca. 4100 yr BP and postdates dark layer WH6 at ca. 5900 yr BP (Fig. 8). In conclusion, although some of the twelve dark layers such as WH9, WH10, and WH11 in the Holocene varnish layering sequence have not been calibrated due to lack of appropriate radiocarbon age control in the study region, our preliminary age calibration presented above indicates that there is generally a temporal correlation between the varnish climate record and the deep sea record. In other words, dark layers WH12-WH9, WH6-WH3, and WH1 in the Holocene varnish layering sequence most likely correlate in time with the Holocene cooling events 8-5, 4-1, and LIA uncovered in the deep sea record; and dark layers WH8, WH7, and WH2 likely correlate in time with minor cold phases around ca. 7300, 6500, 900-1100 yr BP that were not qualified as the Holocene cooling events in the deep sea record based on the criteria of Bond et al. (1997, 1999). 17 3.4. Chronometric application Once established and radiometrically calibrated, varnish microstratigraphy can be used to estimate numerical ages for geomorphic and geoarchaeological features of interest (Liu and Dorn, 1996; Bell et al., 1998b; Friend et al., 2000; Liu, 2003; Douglass et al., 2005). For the purpose of age dating in this study, we tentatively assume that dark layers WH1-WH12 in the Holocene varnish microstratigraphy deposited around 300-650, 9001100, 1400, 2800, 4100, 5900, 6500, 7300, 8100, 9400, 10 300, and 11 100 yr BP, respectively, based on our preliminary age calibration and climatic correlation. Figure 8 gives a graphic presentation of these age assignments. We also like to point out that any varnish-based age estimate should be interpreted as minimum surface exposure age for the varnished geomorphic feature and only as good as its calibration could get. In the following sections, we present several case studies to demonstrate the potential use of varnish microstratigraphy as a unique dating tool in the western US drylands. 3.4.1. Geomorphic use Debris-flow fan deposits, Death Valley Debris-flow fans are common features in arid to semiarid environments. In Death Valley of the Mojave Desert, some ten debris-flow fans of various sizes crop out within a distance of about 1 km on the east side of the valley north of Mormon Point, as illustrated by this most fantastic one that we studied (Fig. 9). Previous studies (Bull, 1991) classified the deposits of this debris-flow fan into five age groups, namely Q2c, Q3a, Q3b, Q3c, and Q4, based largely on differential development of rock varnish on the deposits (see Fig. 2.26B of Bull, 1991). Here, Q2c represents the oldest fan unit with dark varnish coverage, 18 probably formed during the terminal Pleistocene (70-12 ka); Q3a-c represents the subsequent fan units with varying degree of orange varnish coverage, likely formed during the early-to-late Holocene around 12-8, 8-4, 4-2 ka, respectively; and Q4 represents the modern debris-flow deposits that have no varnish coverage, likely formed during the latest Holocene around 2-0 ka (see Table 2.13 of Bull, 1991). However, due to the extreme difficulty in radiometric dating of debris-flow fan deposits, no specific numerical chronology exists for the formation and evolution of this debris-flow fan. In this study, varnish layering dating was used to provide numerical age estimates for the debris-flow fan deposits. Based on fan morphology and varying degree of varnish development, we identified seven debris-flow fan units (i.e., Df1 through Df7), with Df1 being the oldest and Df7 the youngest (Fig. 9). Varnish samples were collected from all but the youngest (i.e., Df7) units for layering pattern analysis and age dating. Table 4 gives a summary of these debris-flow fan units, samples collected on each unit, oldest varnish layering patterns observed, varnish-based age estimates, and their correlation to Bull’s (1991) generalized alluvial-fan units (i.e., Q2c, Q3a-c, and Q4) in the Mojave Desert. Varnish from Df1 and Df2 debris-flow fan units with the darkest/dark varnish coverage (i.e., Q2c) displays, respectively, the oldest pre-Holocene LU-1/LU-2 (WP0) and early Holocene LU-1 (WH12+) layering sequences, suggesting a minimum age of 12 500 yr BP for Df1 and 11 100-12 500 yr BP for Df2 (Figs. 9E,F; see Liu, 2003 for terminology). Varnish from Df3 and Df4 debris-flow fan units with dark/orange varnish coverage (i.e., Q3a) shows early Holocene layering sequences LU-1 (WH12) and LU-1 (WH11) (Figs. 9C,D), indicating a minimum age of 11 100 yr BP for Df3 and 10 300 yr BP for Df4. Varnish from Df5 debris-flow fan units with light orange varnish coverage 19 (i.e., Q3b) displays an early Holocene layering sequence LU-1 (WP10) (Fig. 9B), suggesting a minimum age of 9400 yr BP for Df5. Varnish from Df6 debris-flow fan unit with the lightest orange varnish coverage (i.e., Q3c) shows a late Holocene layering sequence LU-1 (WH4) (Fig. 9A), indicating a minimum age of 2800 yr BP for Df6. It is clear from Table 4 that our preliminary varnish-based age estimates are in close agreement with Bull’s (1991) correlative age assignments for these debris-flow fan units and also have much better age resolution. More importantly, our dating results indicate that debris-flow fan building events in Death Valley of the Mojave Desert are more likely to have occurred during relatively wet periods of the Holocene, as evidenced by the basal dark layers in varnish microstratigraphies from four (out of five) sampled Holocene debris-flow fan units in this case study (Fig. 9). 3.4.2. Geoarchaeological use Grinding Stone, Chili of northern New Mexico Although varnish microstratigraphy method has been previously applied to dating prehistoric rock arts in the Great Basin of western USA (e.g., Dorn,1992; Liu and Dorn, 1996), this dating technique was used for the first time in this study to determine the age of historic stone artifacts in the western US deserts. One varnish sample was obtained from a grinding stone (i.e., metate) near Chili of northern New Mexico (Figs. 2 and 10A). The outcrop of hundreds of Anasazi pottery shards (Pueblo II-III pottery; Liu, unpublished data) from a nearby hillslope implicates a maximum age of 700-1100 yr BP (Lister and Lister, 1978) for the abandonment of this occupation site by the Anasazi Indians. Varnish from the sampled grinding stone displays a latest Holocene layering 20 sequence LU-1 (WH2) (Fig.10B; also see Fig. 6E), suggesting a minimum age of 9001100 yr BP for the varnish initiation on the abandoned metate. This age estimate appears to be consistent with the available archaeological evidence from the sampling site. Stone Artifact, Ocotillo of southern California In order to demonstrate the great potential of varnish microstratigraphy method in dating old prehistoric stone artifacts in the western US deserts, one sample was also obtained from a well-varnished flaked stone (i.e., a primary core) collected by archaeologist Jay von Werlhof near Ocotillo, southern California (Figs. 2 and 10C). This varnish displays a latest Pleistocene layering sequence LU-1/LU-2 (WP0) (Fig.10D; also see Fig. 5C), indicating a minimum age of 12 500 yr BP for the making of this stone artifact and thus suggesting at least a Paleo-Indian human occupation at Ocotillo during the terminal Pleistocene. 4. Discussion As illustrated above, varnish microstratigraphy method is a unique dating tool in studying desert geomorphology and geoarchaeology in the western US drylands. It can be very useful in dating surficial geomorphic features where other radiometric means such as radiocarbon and cosmogenic radionuclide methods are not applicable or difficult to use. However, like any other Quaternary dating techniques in earth sciences, it also has some intrinsic problems that deserve further discussion. 4.1. Validity of age calibration 21 As a correlative dating tool, varnish microstratigraphy method depends largely on radiometric age calibration of layering sequence. In this study, most of the age constraints came from radiocarbon dates (with age uncertainty of less than 200 years), which are accurate and precise enough to differentiate the Holocene millennial-scale wet events recorded in rock varnish (see Figs. 7 and 8). However, there is an intrinsic uncertainty related to our indirect way of radiometric age calibration. Because the dated charcoal or organics are often buried 0.5-5 m underneath the sampled alluvial-fan surfaces (Table 3), there must be a time gap between deposition of the dated materials and deposition of the varnished alluvial boulders. In our age calibration model, this time gap plus a varnish lag time is tentatively assumed to be about ~ 200 years (Liu, 2003). Such assumption may not be true for buildup of large alluvial fans during the last glacial time when long-lasting (on a time scale of ~ 103 years) and extremely wet climate was dominant in the drylands of western USA (Bension et al., 1990; Oviatt et al., 1992). It is however probably reasonable for buildup of small alluvial fans during the interglacial time of the Holocene when shortlived (on a time scale of ~ 102 years) and debris-flow fan building wet climate was prevalent (Miller et al., 2001). One piece of circumstantial evidence from this study appears to support such claim. For all of the radiocarbon dated Holocene alluvial-fan surfaces in the study region, if we take 200 years of varnish lag time plus the depositional time gap into consideration, the radiometric age constraints available for the basal dark layers of Holocene varnish samples almost perfectly match the corresponding climatic correlation-based age assignments of these dark layers; otherwise, the former would be systematically older than the latter for about 200 years (Table 3). Therefore, this evidence 22 further indicates that our age calibration of the Holocene layering sequence is probably as good as radiocarbon dating could get for the varnish climate record. 4.2. Validity of varnish climate record As a correlative dating tool, varnish microstratigraphy method also depends on a premise that climate variations are recorded in varnish. Based on our preliminary radiometric age calibration, we found that the Holocene climate record in rock varnish is generally valid and comparable to other climate records in the study region (Table 5). In the Mojave Desert, for example, Enzel et al. (1989) reported three Holocene wet periods in the lacustrine record of Silver Lake. The first wet period occurred around 10 540 ± 140 yr BP, fairly in accord with the early Holocene wet phase WH11 presumably at 10 300 yr BP. The second one occurred around 3930 ± 100 yr BP, closely tied to the late Holocene wet phase WH5 at 4100 yr BP. The third one occurred around 440 ± 100 yr BP, largely overlapped the most recent wet phase WH1 around 300-650 yr BP. Miller et al. (2001) documented a wet period in the middle Holocene at 6500 ± 500 yr BP (Infrared Stimulated Luminescence or IRSL age) that was concurrent with paired debris-flow building and a short-lived lake stand in the northern Silurian Valley of the Mojave Desert. This wet period correlates favorably with the middle Holocene wet phase WH7 presumably at 6500 yr BP in the varnish record (Fig. 8). Other regional climate proxies, such as a pollen record from San Joaquin Marsh (Davis, 1992), a flow discharge record from Sacramento River (Meko et al., 2001; also see Yuan et al., 2004), lake sediment records from Walker Lake (Adams, 2005), Owens and Pyramid Lakes (Benson et al., 2002), black mat records from southern Nevada (Quade et al., 1998) and Silurian Valley 23 (Anderson and Wells, 2003), and morainal and lake sediment records of Holocene glacier advances in the Sierra Nevada (Curry, 1969; Bowerman, 2005), provide complementary Holocene wetness records in the western US drylands that nearly perfectly correlate in time with the twelve Holocene wet phases in the varnish record (see Table 5 for interrecord correlation). It is also interesting to mention that varnish microstratigraphy may have the potential to carry a high-resolution (centennial scale) Holocene wetness record in the western US drylands. As illustrated in Fig.11 (right panel), one fine-grained and fastgrowing varnish sample from radiocarbon dated alluvial-fan surfaces in Leidy Creek of Fish Lake Valley (1510 ± 70 yr BP; Table 3) hosts a high-resolution optical microstratigraphy. Here, dark layer WH1 contains three secondary dark layers (namely, WH1a, WH1b, and WH1c) and dark layer WH2 contains two secondary dark layers (namely, WH2a and WH2b); each of these secondary dark layers further consists of 2-4 narrowly-spaced dark bands. Given a lag time of ~ 200 years for varnish initiation on the sampled alluvial boulder, the basal dark layer WH3 in the microstratigraphy likely deposited around 1310 ± 70 yr BP. If these dark layers are interpreted as a climatic proxy of wetness (Liu et al., 2000; Broecker and Liu, 2001; Liu, 2003), the varnish microstratigraphy provides a centennial-scale wetness record of the past 1300-1400 years for Fish Lake Valley of Nevada. A comparison of this varnish record with a nearby 2000year long Mono Lake record (Stine, 1990) suggests a fascinating correlation (Fig.11). Specifically, three secondary dark layers WH1a, WH1b, and WH1c in the varnish record likely correlate in time with three LIA highstands (HS) of Mono Lake (i.e., Clover Ranch HS, Danberg Beach HS, and Rush Delta HS), one secondary dark layer WH2a with an 24 Medieval Warm Period (MWP) (ca. 800-1200 yr BP; Soon et al., 2003) highstand (i.e., Post Office HS), and one major dark layer WH3 with a pre-MWP highstand (i.e., Mill Creek-East HS). The secondary dark layer WH2b in the varnish record may correlate in time with a short-lived wet period (~ 1100 yr BP) during the MWP that has been well documented in other regional climate records from the western US drylands (Table 5), but such wet period is not discernible in the Mono Lake record (Fig. 11). Clearly, without detailed radiocarbon age calibration of these dark layers, the above mentioned correlation between the two climate records is at best conjectural. However, if such correlation indeed exists, dark layers WH1a, WH1b, and WH1c are most likely to be deposited respectively around ca. 300, 500, and 650 yr BP, and dark layers WH2a and WH2b around ca. 900 and 1100 yr BP (Fig. 11; Table 5). More studies in the future are desperately needed to ascertain this correlation. 5. Conclusion Rock varnish microstratigraphy is a useful geomorphic and geoarchaeological dating tool. Without age calibration, varnish microstratigraphy can stand along as a regional correlation and mapping tool (Liu, 1994; Liu and Dorn, 1996). With radiometric age calibration, it can provide numerical age constraints on surficial geomorphic and geoarchaeological features in the drylands of western USA, with a possible age resolution of 300-500 years for the Holocene and a few thousand years during the late Pleistocene (Liu, 2003). Moreover, when detailed radiometric age calibration is accomplished for the Holocene varnish layering sequence, the technique has the potential to yield numerical age assignments for surface stone tools, petroglyphs, and geoglyphs that may be 25 correlated to the major cultural periods and phases of American prehistory in the western U.S. deserts. Acknowledgements This research was funded by the US Department of Energy (DE-FG0295ER14572). The chemical analyses of rock varnish samples were conducted by a CAMECA SX100 electron probe at the American Museum of Natural History in New York City. We thank the late Gerard Bond for stimulating discussion on chronology of Holocene cooling events; Charles Mandeville for assistance in obtaining the probe data; George Kukla and Richard Alley for comments on the paleoclimatic aspects of this paper. We thank Thure Cerling, Dorothy E. Freidel, and the late Jay von Werlhof for providing varnish samples for microstratigraphic analysis. Thanks also go to Moanna St. Clair for her decade-long support and help in managing this DOE project. Last but not least, the first author likes to thank Ping Zhou and Marisa Liu for their assistance in numerous field trips to the Great Basin and for their understanding of the importance of this work. Without their continued appreciation and support, this work could have never been completed. 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A 1200 year record of hydrologic variability in the Sierra Nevada from sediments in Walker Lake, Nevada. Geochemistry, Geophysics, Geosystems 5, Q03007. 33 Figure Captions Figure 1. Electron microprobe element maps (128 128 m) of rock varnish from Death Valley, California. The inset image at lower right is an optical microstratigraphy seen in varnish ultra-thin section under a polarized light microscope. As seen from the probe maps, silica (Si), aluminum (Al), magnesium (Mg), and potassium (K) achieve their highest concentrations in the yellow layer at the top of the varnish microstratigraphy (interpreted to represent the Holocene; Liu, 2003). In contrast, maganese (Mn), barium (Ba), calcium (Ca), and phosphorus (P) achieve their highest concentrations in the dark layers (interpreted to represent the last glacial time; Liu, 2003). Iron (Fe) shows the smallest glacial to interglacial change in concentration. With some exceptions, sulphur (S) is not generally associated with barium in varnish microlaminae during both glacial and interglacial time. The line profile of barium was taken along a vertical traverse and the probe mapped portion of the varnish microstratigraphy is marked by a square. This varnish contains a layering sequence of LU-1/LU-2/LU-3/LU-4 (WP6), suggesting a minimum varnish-based age estimate of 60,000 yr BP (see Liu, 2003 for more discussion on layering pattern interpretation of rock varnish). Figure 2. Location map of rock varnish sampling sites in the drylands of western USA. Abbreviations of some localities are: Death Valley (DV), Owens Valley (OV), Panamint Valley (PV), Searles Lake (SL), Silver Lake and Silurian Valley (SV). 34 Figure 3. Replicated Holocene varnish microstratigraphies in the drylands of western USA. Varnish samples in (A), (B), (D), and (F) were from latest Pleistocene highstand shorelines of Searles Lake, Panamint Lake, Lahontan Lake, and Summer Lake. Varnish in (C) was from latest Pleistocene alluvial-fan surfaces in McDowell Mountain of Arizona, and the one in (E) from the Dry Falls of Owens Valley, California. These fast-growing and younger varnish samples were intentionally collected from the older latest Pleistocene geomorphic surfaces for observation of a complete Holocene varnish microstratigraphy (except for (F) that was collected by D.E. Freidel). Age constraints are maximum-limiting ages for varnish (reported in cal yr BP), and age sources are given in Table1. WH = Wet event in Holocene, and WP = Wet event in Pleistocene. See Table 2 and text for discussion. Figure 4. Rock varnish record of the Holocene wet phases (WH1-WH12) in the drylands of western USA and its possible correlation with deep sea sediment record of the Holocene millennial-scale cooling events (LIA and 1-8) in the subpolar North Atlantic region. WH = Wet event in Holocene, WP = Wet event in Pleistocene, LIA = Little Ice Age, and YD = Younger Dryas. Figure 5. Optical (left) and chemical (right) microstratigraphies in rock varnish from three localities along an N-S traverse in the drylands of western USA. Note that Mn and Ba contents in the Holocene portion of each varnish microstratigraphy are much lower than that in the terminal Pleistocene portion of the microstratigraphy. Also note that the average Holocene Mn and Ba contents in the varnish exhibit a general trend 35 along the traverse, from the lowest in Death Valley to the highest in Ocotillo, which appears to reflect the present-day effective moisture gradient in southern California (see text for discussion). WH1-WH12 and WP0 in the right panel identify Mn-rich peaks on each probe line profile that correspond to the Holocene and terminal Pleistocene dark layers seen in the optical microstratigraphies. WH = Wet event in Holocene, WP = Wet event in Pleistocene. Figure 6. Chemical microstratigraphies of Ba in rock varnish of different age and intersample correlation of Holocene dark layers WH1-WH12 as represented by Ba peaks. Note that the vertical axis of varnish thickness is on the same scale for all varnish samples except for (C) that is on a different scale, as labeled in the figure. Age constraints for (C) and (D) are from Table 3, and those for (A), (B), and (E) are from Figs. 9 and 10. Figure 7. Optical microstratigraphies in rock varnish from radiometrically dated geomorphic features in the western US drylands. Age constraints are reported in cal yr BP (± 1) and sources of age control are given in Table 3. Letters in the varnish images correspond to those in both Table 3 and Fig. 8. Note that black layer WH7 in (N) is missed due probably to postdepositional modifications such as wind abrasion or peeling (see Liu, 2003, for more discussion). The green lines in (C) and (L) identify positions of probe line profile for Ba seen in Figs. 6C, D. WH = Wet event in Holocene, WP = Wet event in Pleistocene. 36 Figure 8. A generalized Holocene varnish layering sequence in the drylands of western USA and its preliminary radiometric age calibration. The assumed age assignments for dark layers in the varnish layering sequence are based on a possible correlation between the Holocene wet events recorded in rock varnish and the Holocene millennial-scale cooling events recorded in deep sea sediments from the subpolar North Atlantic region (Fig. 4; Bond et al., 1999). Radiometric age constraints are from Table 3 and the letters are keyed to those in both Fig. 7 and Table 3. Figure 9. A debris-flow fan on the east side of Death Valley, California (upper panel; creosote bushes of ~ 2-m high in the foreground for scale) and optical microstratigraphies of rock varnish from the debris-flow fan deposits (lower panel). Seven debris-flow fan units (Df1-Df7) were identified on the basis of fan morphology and degree of varnish coverage (see Table 4). Varnish-based age estimates (reported in cal yr BP) indicate that placement of these fan units are more likely to have occurred during relatively wet periods of the Holocene. Note that while optically similar due to slightly greater thickness of varnish ultra-thin sections, the Holocene dark layers in these images often contain less barium than the pre-Holocene dark layers, as illustrated in (C) and (F). The brown burning marks in (C) were made by electron microprobe during line profiling. Figure 10. Varnish layering dating of stone artifacts in the drylands of western USA. (A) A varnished grinding stone from Chili of northern New Mexico, where numerous Anasazi pottery shards were found. (B) Rock varnish from the grinding stone in (A) 37 displays a latest Holocene layering sequence LU-1 (WH2), suggesting a varnish-based minimum age of 900-1100 yr BP for abandonment of this occupation site by the Anasazi Indians. (C) A varnished primary stone core from Ocotillo of southern California (collected by Jay von Werlhof, Desert Museum of Imperial Valley College). (D) Rock varnish from the primary core in (C) displays a latest Pleistocene layering sequence LU-1/LU-2 (WP0), suggesting a varnish-based minimum age of 12 500 yr BP for the making of this stone artifact by the Paleo-Indians. A green line in (B) identifies position of probe line profile for Ba seen in Fig. 6E. A camera cap in (A) is for scale; arrows in (A) and (C) point to sites where varnish-filled microbasins were cored with a mini-drill (4 mm in diameter) for dating. Figure 11. A possible correlation of rock varnish climate record with Mono Lake record. The varnish record was from Leidy Creek of Fish Lake Valley, NV, with radiometric age control of 1310 ± 70 yr BP for the basal dark layer of WH3 (Fig. 7D; Table 3). The Mono Lake record was from Stine (1990). Note that the LIA wet phases of WH1a, WH1b, and WH1c recorded in varnish likely correlate in time with the Clover Ranch HS, Danberg Beach HS, and Rush Delta HS of Mono Lake at ca. 350, 500, 650 yr BP, respectively. Similarly, wet phase WH2a likely correlates with the Post Office HS at ca. 900 yr BP during the Medieval Warm Period, and wet phase WH3 with the Mill Creek-East HS at ca. 1375 yr BP. Wet phase WH2b may be concurrent with the 1100-yr BP wet/cooling event in the western US drylands (see Table 5) that is however not discernible in the Mono Lake record. HS = Highstand, LIA = Little Ice Age, and WH = Wet event in Holocene. 38