Curriculum Vitae
Ioannis V. Zaganas, MD, PhD
Heraklion, Crete, November 2010
Personal Information
http://www.neurologycrete.gr/index.php/el/zaganasioannis
Date of birth:
Address:
Tel:
Ε-mail:
May 16th, 1973
Voutes, 70013, Heraklion, Crete, Greece
+30-2810721493, +30-6946060360
johnzag@med.uoc.gr, johnzag@yahoo.com
Education
1991-1997: University of Crete, Medical School (MD Degree)
Postgraduate training and professional appointments
(1)
(2)
(3)
(4)
(5)
Doctoral Dissertation (1999-2005): «Glutamate dehydrogenase in the biology of the
mammalian nervous system». Medical School, University of Crete (Advisor: Professor A.
Plaitakis)
Postdoctoral Fellow (2004-2005): Mount Sinai School of Medicine, Department of
Psychiatry and Neurobiology (New York, USA), on Genetics and Neurobiology of
Alzheimer’s Disease (Supervisor: Professor N. Robakis)
Neurology Residency (2005-2008): Neurology Department, University Hospital of
Heraklion, Crete (Neurology Certification on October 2010)
Internal Medicine Residency (1999): Internal Medicine Department, University Hospital of
Heraklion, Crete
Psychiatry Residency (2000): Psychiatry Department, University Hospital of Heraklion,
Crete
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(6)
(7)
Neurology Consultant, Neurology Department, University Hospital of Heraklion, Crete
(2010)
Rural Service, Kastelli Health Center, Crete (1997-1999)
Faculty appointments
(1)
(2)
Temporary Lecturer of Neurology (Law PD407), University of Crete, Medical School
(2009-2010)
Temporary Assistant Professor of Neurology (Law PD407), University of Crete, Medical
School (2008-2009)
Scholarships
(1)
(2)
(3)
(4)
(5)
Scholarship from the “A. Onassis” foundation for training in Mount Sinai Hospital, New
York (2004-2005)
Scholarship “M. Manassaki” (University of Crete) for postgraduate studies (2001-2003)
Scholarships from the Association for Research and Treatment of Neurological Disorders
of Crete “EY ZHN” (1999-2009)
Scholarship from the Erasmus program (pre-graduate training in Pediatrics in Lyon,
France) (1996)
Greek State Scholarships (IKY) for being within the top students in Medical School (19921994)
Molecular Biology Techniques and other skills
(1)
(2)
Cell cultures (mammalian and insect cells)
Cloning, mutagenesis, protein expression in bacteria, insect cells, mammalian cells (EGFP,
retroviral and Baculovirus systems, etc)
(3) Enzyme kinetics, protein purification (chromatography systems), structural modeling
software (Pymol, Swiss PDB viewer, etc)
(4) SDS PAGE, Western blot, Immuno-fluorescence, Confocal microscopy, Coimmunoprecipitation
(5) Cellular biology studies (cell survival pathways, apoptosis studies, kinase pathways, etc)
(6) Mouse neuronal primary cultures
(7) Production and characterization of polyclonal antibodies
(8) PCR, Sequencing (ABI 3100 etc), Fragment Analysis
(9) Genetic studies: Linkage analysis, Association studies
(10) Statistical software (Origin, SPSS, Excel, etc)
(11) English, French (fluently)
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Publications in peer-reviewed journals:
(1)
Zaganas I, Plaitakis A. Single amino acid substitution (G456A) in the vicinity of the GTP
binding domain of human housekeeping glutamate dehydrogenase markedly attenuates
GTP inhibition and abolishes the cooperative behaviour of the enzyme. J Biol Chem 277:
26422-26428, 2002.
(2) Zaganas I, Spanaki C, Karpusas M, Plaitakis A. Substitution of Ser for Arg443 in the
regulatory domain of human housekeeping (GLUD1) glutamate dehydrogenase virtually
abolishes basal activity and markedly alters the activation of the enzyme by ADP and Lleucine. J Biol Chem 277: 46552-46558, 2002.
(3) Spanaki C*, Zaganas I*, Kleopa K, Plaitakis A. Human GLUD2 glutamate dehydrogenase
is expressed in neural and testicular supporting cells. J Biol Chem 2010 (in press). *equal
contribution
(4) Xu J, Litterst C, Georgakopoulos A, Zaganas I, Robakis NK. Peptide EphB2/CTF2
generated by the gamma-secretase processing of EphB2 receptor promotes tyrosine
phosphorylation and cell surface localization of N-methyl-D-aspartate receptors. J Biol
Chem 284: 27220-27228, 2009.
(5) Zaganas I, Waagepetersen HS, Georgopoulos P, Sonnewald U, Plaitakis A, Schousboe A.
Differential expression of glutamate dehydrogenase in cultured neurons and astrocytes
from mouse cerebellum and cerebral cortex. J Neurosci Res 66: 909-913, 2001.
(6) Zaganas I, Prinianakis G, Xirouchaki N, Mavridis M. Opsoclonus-myoclonus syndrome
associated with Cytomegalovirus encephalitis. Neurology 68: 1636, 2007.
(7) Zaganas Ι, Latsoudis Η, Papadaki Ε, Vorgia P, Spilioti M, Plaitakis A. A8344G tRNALys
Mutation Associated with Recurrent Brain Stem Stroke-like Episodes. J Neurol 256: 271273, 2009.
(8) Zaganas I, Kanavouras K, Mastorodemos V, Latsoudis H, Spanaki C, Plaitakis A. The
human GLUD2 glutamate dehydrogenase: localization and functional aspects. Neurochem
Int 55: 52-63, 2009.
(9) Plaitakis A, Zaganas I. Regulation of Human Glutamate Dehydrogenases: Implications
for Glutamate, Ammonia and Energy Metabolism in Brain. J Neurosci Res 66: 899-908,
2001.
(10) Plaitakis A, Spanaki C, Mastorodemos V, Zaganas I. Study of Structure-Function
Relationships in Human Glutamate Dehydrogenases Reveals Novel Molecular Mechanisms
for the Regulation of the Nerve Tissue-Specific (GLUD2) Isoenzyme. Neurochem Int 43:
401-410, 2003.
(11) Mastorodemos V, Zaganas I, Spanaki C, Bessa M, Plaitakis A. Molecular basis of human
glutamate dehydrogenase regulation under changing energy demands. J Neurosci Res 79:
65-73, 2005.
(12) Saloustros E, Zaganas I, Mavridis M, Vamvakas L, Plaitakis A, Georgoulias V, Mavroudis
D. Anti-CV2 associated cerebellar degeneration after complete response to chemoradiation
of head and neck carcinoma. J Neurooncol 97: 291-294, 2010.
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(13) Mastorodemos V, Kotzamani D, Zaganas I, Arianoglou G, Latsoudis H, Plaitakis A.
Human GLUD1 and GLUD2 glutamate dehydrogenase localize to mitochondria and
endoplasmic reticulum. Biochem Cell Biol 87: 505-516, 2009.
(14) Kanavouras K, Borompokas N, Latsoudis H, Stagourakis A, Zaganas I, Plaitakis A.
Mutations in human GLUD2 glutamate dehydrogenase affecting basal activity and
regulation. J Neurochem 109 (Sup. 1): 167-173, 2009.
(15) Plaitakis A, Latsoudis H, Kanavouras K, Ritz B, Bronstein JM, Skoula I, Mastorodemos V,
Papapetropoulos S, Borompokas N, Zaganas I, Xiromerisiou G, Hadjigeorgiou GM,
Spanaki C. Gain-of-function variant in GLUD2 glutamate dehydrogenase modifies
Parkinson's disease onset. Eur J Hum Genet 18: 336-341, 2010.
(16) Plaitakis A, Viskadouraki K, Tzagournissakis M, Zaganas I, Verghese-Nikolakaki S,
Karagiorgis V, Panagiotides I, Kilindireas C, Patsouris E, Haberler C, Budka H, Sklaviadis
T. Increased incidence of Sporadic Creutzfeldt-Jakob disease on the island of Crete
associated with a high rate of PRNP 129 methionine homozygosity in the local population.
Ann Neurol 50: 227-233, 2001.
(17) Kofteridis DP, Karabekios S, Repa A, Zaganas I, Anastasopoulos T, Barbounakis E, Gikas
A. A case of human immunodeficiency virus infection disclosed by cytomegalovirus
encephalitis. Int J Infect Dis. 11:373-5, 2007.
(18) Borompokas N, Papachatzaki MM, Kanavouras K, Mastorodemos V, Zaganas I, Spanaki
C, Plaitakis A. Estrogen modification of human glutamate dehydrogenases is linked to
enzyme activation state. J Biol Chem, 285: 31380-7, 2010.
(19) Papapanagiotou P, Iacovidou N, Spengos K, Xanthos T, Zaganas I, Aggelina A,
Alegakis A, Vemmos K. Temporal trends and associated factors for prehospital and in
hospital stroke-related delay over a 16-year period: the Athens Study. Cerebrovasc Dis (in
press), 2010.
Selected abstracts
(1)
(2)
(3)
Zaganas I, Halpin AP, Oleink A, Kotzamani D, Chlapoutaki C, Tsimoulis D, Zafiris S,
Giannakoudakis E, Alegakis A, Papadaki E, Furie KL, Greenberg SM, Plaitakis A. A
Comparison of Acute Hemorrhagic Stroke Outcomes in Two Populations: The Crete-Boston
Study. International Stroke Conference 2010, San Antonio, Texas (February 24-26, 2010).
Stroke 41 (4): e13.
Zaganas I, Latsoudis H, Papadaki E, Vorgia P, Spilioti M, Plaitakis A. Mitochondrial DNA
A8344G tRNALys mutation associated with recurrent migratory brainstem stroke-like episodes.
132nd Annual Meeting of the American Neurological Association, 7-10 October 2007,
Washington DC, United States. Ann Neurol 62 (suppl. 11): S47, 2007.
Zaganas I, Saloustros E, Vamvakas L, Mavridis M. Paraneoplastic cerebellar degeneration
associated with tongue cancer and anti-CV2 auto-antibodies. Poster Presentation at the 18th
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Meeting of the European Neurological Society (7 - 11 June 2008, Nice, France). J Neurol 255
(suppl. 2): S153, 2008.
(4) Zaganas I, Latsoudis H, Spilioti M, Tzagournissakis M, Amoiridis G, Plaitakis A. Title: Novel
spastin mutation (IVS14+5G > A) in a Greek family with hereditary spastic paraplegia. Poster
Presentation at the 18th Meeting of the European Neurological Society (20 - 24 June 2009,
Milan, Italy). J Neurol 256 (suppl. 2): S156, 2009.
(5) Plaitakis A, Viskadouraki A, Tzagournissakis M, Zaganas JV, Panayiotides J, Manousis T,
Verghese-Nikolakaki S, Patsouris E, Haberler C, Budka H, Sklaviadis T. Increased incidence of
sporadic Creutzfeldt-Jacob disease on the Island of Crete associated with a reduced rate of codon
129 Methionine/Valine heterozygosity in the local population. 52nd American Academy of
Neurology Annual Congress 2000, San Diego, CA, United States. Neurology 2000; 54 (suppl 3):
A285.
(6) Spanaki C, Zaganas I, Plaitakis A. Frequent familial occurrence of late onset, Dopa responsive
Parkinson’s disease on the island of Crete. 52nd American Academy of Neurology Annual
Congress 2000, San Diego, CA, United States. Neurology 2000; 54 (suppl 3): A263
(7) Plaitakis A, Zaganas I. Allosteric regulation of human glutamate dehydrogenase, derived
from the GLUD1 (housekeeping) and the GLUD2 genes (nerve tissue specific), and its
implications for glutamate metabolism under changing energy demands. 5th International
Conference on Brain Energy Metabolism. Trondheim, Norway, 19-22/5/2001.
(8) Plaitakis A, Zaganas I. Gly456 and Arg443 are essential for allosteric regulation of human
GLUD1 glutamate dehydrogenase. 33rd Annual Meeting of the American Society for
Neurochemistry, 22-26 June 2002, Palm Beach, Florida, United States. J Neurochem 81
(suppl 1): 26, 2002.
(9) Tzifa G, Kalikakis G, Zaganas I, Spilioti M, Kofteridis D, Daraki V, Petkova V,
Tzoutzouraki P, Papavasileiou S. Secondary hypothalamic amenorrhea as the initial
manifestation of HIV infection. 9th European Congress of Endocrinology, 28 April - 2 May
2007, Budapest, Hungary.
(10) Zaganas I, Spanaki C, Plaitakis A. Structure-function relationship of brain-specific
glutamate dehydrogenase, as studied by site-directed mutagenesis, reveals that Ser443 and
Ala456 are critical for enzyme regulation in nerve tissue. 17th meeting of the Greek Society
for Neuroscience: “Brain, Mind and Behavior”, 4-6 October 2002, Rethimnon, Crete,
Greece.
(11) Spanaki C, Mastorodemos V, Zaganas I, Plaitakis A. Purified nerve tissue-specific
glutamate dehydrogenase has properties adapted to the metabolism of excitatory
neurotransmitter glutamate. 17th meeting of the Greek Society for Neuroscience: “Brain,
Mind and Behavior”, 4-6 October 2002, Rethimnon, Crete, Greece.
(12) Evangeliou A, Varakis N, Spilioti M, Michailidou H, Sofokleous C, Frysira H, Zaganas I,
Tzoufi M, Nikolaidou P. Autism and inborn errors of metabolism. Are we effective with
the diagnosis? 1st Aegean Meeting on Neurologic Therapeutics, September 11-15, 2004,
Heraklion, Crete, Greece.
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(13) Mastorodemos V, Zaganas I, Petsakou A, Plaitakis A. Subcellular localisation of human
GLUD1 and GLUD2-derived glutamate dehydrogenase. 2nd Aegean Meeting on Neurologic
Therapeutics, May 20-23, 2006, Heraklion, Crete, Greece.
(14) Kanavouras K, Spanaki C, Zaganas I, Plaitakis A. Substitution of Serine 445 by Alanine in
the GLUD2-gene encoding neural tissue specific glutamate dehydrogenase associated with
early onset Parkinson’s disease in male hemizygotic carriers. 2nd Aegean Meeting on
Neurologic Therapeutics, May 20-23, 2006, Heraklion, Crete, Greece.
Invited speaker in meetings
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(2)
Clinical picture and pathophysiology of Dementia: Nuclear Medicine / Neurology
Conference, Heraklion (November 24th, 2007).
Human glutamate dehydrogenases: structure and function: Glutamate in the tripartite
synapse: functional and metabolic relations in norm and pathology Conference, Wierzba
(Mazurian lakes, Poland) (23-27 August 2008).
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