Guanidinium chloride.
2-mercaptoethanol.
Tween 80.
Chloroform.
Hazardous Substances Policy - Assessment
CHEMICAL HAZARD AND RISK ASSESSMENT
School of Biosciences
Name of
supervisor
G. S. Besra
Assessor
D. Lonergan
Assessment Number*
2019
Date of Assessment
22/04/02
Signature
Signature
Notes
A School COSHH form in Word is available on the School Server.
Available from the Health and Safety Unit.
Guidance on making an assessment is given in Making a Chemical Hazard and Risk Assessment.
Guidance is also available from Guidance on Completing the Chemical Hazard and Risk Assessment Form.
Use a continuation sheet to expand any section of this form in hard copy version.
1
LOCATION OF THE WORK ACTIVITY
2
PERSONS WHO MAY BE AT RISK
E114
List names where possible
D. Lonergan.
L.G.Dover
3
ACTIVITY ASSESSED
4
MATERIALS INVOLVED
NAME
Isolation of Mycobacterial DNA.
AMOUNT
max/stored
Attach copies of data sheet(s)
HAZARD
Guanidinium chloride.
100 g
HARMFUL.
2-mercaptoethanol.
500 ml
TOXIC.
Tween 80.
500 ml
RISK PHRASES
HARMFUL IF
SWALLOWED
IRRITATING TO EYES
AND SKIN. R: 22-36/38; S:
22
TOXIC IN CONTACT
WITH SKIN.
HARMFUL BY
INHALATION AND IF
SWALLOWED.
IRRITATING TO EYES,
RESPIRATORY SYSTEM
AND SKIN. READILY
ABSORBED THROUGH
SKIN. COMBUSTIBLE
LIQUID. STENCH. TOXIC
IF INHALED.R: 24-20/2236/37/38
S: 26-36/37/39-45
MAY CAUSE SKIN
IRRITATION. MAY BE
HARMFUL IF ABSORBED
THROUGH THE SKIN.
MAY CAUSE EYE
IRRITATION. MAY BE
HARMFUL IF INHALED.
MATERIAL MAY BE
IRRITATING TO MUCOUS
MEMBRANES AND
UPPER RESPIRATORY
TRACT.
MAY BE HARMFUL IF
SWALLOWED.
HAZDAT NO***
BIOSCIENCESNO***
Chloroform.
2.5 l
HARMFUL.
FLAMMABLE
.
Isoamyl alcohol.
500 ml
FLAMMABLE
. HARMFUL.
5
HARMFUL IF
SWALLOWED.
IRRITATING TO EYES
AND SKIN. POSSIBLE
RISK OF IRREVERSIBLE
EFFECTS. DANGER OF
SERIOUS DAMAGE TO
HEALTH BY PROLONGED
EXPOSURE THROUGH
INHALATION AND IF
SWALLOWED. POSSIBLE
MUTAGEN. R: 22-38-4048/20/22
S: 36/37
HARMFUL IF
SWALLOWED. RISK OF
SERIOUS DAMAGE TO
EYES. IRRITATING TO
RESPIRATORY SYSTEM
AND SKIN. READILY
ABSORBED THROUGH
SKIN.R: 10-22-41-37/38
S: 16-26-36
INTENDED USE**
Give brief details and attach protocol/instructions
Guanidinium chloride: to be mixed with bacterial suspensions with gentle heating (65 c) and cooling several times in closed
containers.
2-mercaptoethanol: to be mixed with bacterial suspensions with gentle heating (65 c) and cooling several times.
Tween 80: to be mixed with bacterial suspensions with gentle heating (65 c) and cooling several times in closed containers.
Chloroform: to be mixed with chilled bacterial suspensions in closed containers.
Isoamyl alcohol: to be mixed with chilled bacterial suspensions in closed containers.
6
RISKS to HEALTH and SAFETY from INTENDED USE
From personal exposure or hazardous reactions. Refer to OELs, flash points, etc., as appropriate. Are pregnant women, breast-feeding
mothers especially at risk?
Gaunidinium chloride: CAUSES SKIN IRRITATION. MAY BE HARMFUL IF ABSORBED THROUGH THE SKIN.
CAUSES EYE IRRITATION. MAY BE HARMFUL IF INHALED. MATERIAL MAY BE IRRITATING TO MUCOUS
MEMBRANES AND UPPER RESPIRATORY TRACT. HARMFUL IF SWALLOWED.
2-mercaptoethanol. CAUSES SKIN IRRITATION. READILY ABSORBED THROUGH SKIN. HIGHLY TOXICABSORPTION. CAUSES EYE IRRITATION. TOXIC IF INHALED. MATERIAL IS IRRITATING TO MUCOUS
MEMBRANES AND UPPER RESPIRATORY TRACT. TOXIC IF SWALLOWED. FLASH POINT: 73 C.
Tween 80: MAY CAUSE SKIN IRRITATION. MAY BE HARMFUL IF ABSORBED THROUGH THE SKIN. MAY
CAUSE EYE IRRITATION. MAY BE HARMFUL IF INHALED. MATERIAL MAY BE IRRITATING TO MUCOUS
MEMBRANES AND UPPER RESPIRATORY TRACT.
MAY BE HARMFUL IF SWALLOWED.
Chloroform: EXPOSURE CAN CAUSE: VOMITING GASTROINTESTINAL DISTURBANCES. EXPOSURE TO
AND/OR CONSUMPTION OF ALCOHOL MAY INCREASE TOXIC EFFECTS. MAY CAUSE NERVOUS SYSTEM
DISTURBANCES. MAY CAUSE SKIN IRRITATION. MAY BE HARMFUL IF ABSORBED THROUGH THE SKIN.
MAY CAUSE EYE IRRITATION. MATERIAL MAY BE IRRITATING TO MUCOUS MEMBRANES AND UPPER
RESPIRATORY TRACT. MAY BE HARMFUL IF INHALED. MAY BE HARMFUL IF SWALLOWED.
Isoamyl alcohol: CAUSES SKIN IRRITATION. READILY ABSORBED THROUGH SKIN. MAY BE HARMFUL IF
ABSORBED THROUGH THE SKIN. CAUSES SEVERE EYE IRRITATION. MAY BE HARMFUL IF INHALED.
MATERIAL IS IRRITATING TO MUCOUS MEMBRANES AND UPPERRESPIRATORY TRACT. HARMFUL IF
SWALLOWED. PROLONGED CONTACT CAN CAUSE: DIZZINESS, NARCOTIC EFFECT, NAUSEA, HEADACHE
AND VOMITING COUGHING, CHEST PAINS, DIFFICULTY IN BREATHING.
7
CONCLUSIONS ABOUT RISKS
Is level of risk acceptable? Can risk be prevented or reduced by change of substance/procedure? Are control measures necessary?
Isoamyl alcohol; Chloroform; Tween 80; 2-mercaptoethanol; Gaunidinium chloride: The level of risk is acceptable. No
alternative chemicals are suitable. Control measures are required, see below.
8
CONTROL MEASURES
Additional to Good Chemical Practice
Gaunidinium chloride: WORK IN A FUME HOOD. AVOID CONTACT WITH EYES, SKIN AND CLOTHING. AVOID
PROLONGED OR REPEATED EXPOSURE. USE COMPATIBLE CHEMICAL-RESISTANT GLOVES. CHEMICAL
SAFETY GOGGLES. STORE IN A COOL DRY PLACE. KEEP TIGHTLY CLOSED.
2-mercaptoethanol: WASH THOROUGHLY AFTER HANDLING. DO NOT BREATHE VAPOR. AVOID CONTACT
WITH EYES, SKIN AND CLOTHING. AVOID PROLONGED OR REPEATED EXPOSURE. USE COMPATIBLE
CHEMICAL-RESISTANT GLOVES. CHEMICAL SAFETY GOGGLES. USE ONLY IN A CHEMICAL FUME HOOD.
KEEP TIGHTLY CLOSED. KEEP AWAY FROM HEAT AND OPEN FLAME. STORE IN A COOL DRY PLACE.
Tween 80: AVOID INHALATION. AVOID CONTACT WITH EYES, SKIN AND CLOTHING. COMPATIBLE
CHEMICAL-RESISTANT GLOVES. CHEMICAL SAFETY GOGGLES. KEEP TIGHTLY CLOSED. STORE IN A
COOL DRY PLACE.
Chloroform: WORK IN A FUME HOOD. DO NOT BREATHE VAPOR. DO NOT GET IN EYES, ON SKIN, ON
CLOTHING. AVOID PROLONGED OR REPEATED EXPOSURE. USE COMPATIBLE CHEMICAL-RESISTANT
GLOVES. CHEMICAL SAFETY GOGGLES. STORE IN A COOL DRY PLACE. KEEP TIGHTLY CLOSED.
Isoamyl alcohol: DO NOT BREATHE VAPOR. AVOID CONTACT WITH EYES, SKIN AND CLOTHING. AVOID
PROLONGED OR REPEATED EXPOSURE. USE COMPATIBLE CHEMICAL-RESISTANT GLOVES. CHEMICAL
SAFETY GOGGLES. KEEP TIGHTLY CLOSED. KEEP AWAY FROM HEAT AND OPEN FLAME. STORE IN A
COOL DRY PLACE.
9
INSTRUCTION/TRAINING
Specify course(s) and/or special arrangements.
Isoamyl alcohol; Chloroform.Tween 80; Gaunidinium chloride; 2-mercaptoethanol:NEW OPPERATOR TO BE TRAINED
BY REPONSABLE MEMBER OF STAFF.
10
MONITORING
Performance of control measures,
Isoamyl alcohol; Chloroform; Tween 80; Gaunidinium chloride; 2-mercaptoethanol: NONE
Personal exposure
11
Health Surveillance
WASTE DISPOSAL PROCEDURE
See School Server for Approved Procedure Document on specific Chemical Waste Disposal.
Isoamyl alcohol;Tween 80; 2-mercaptoethanol; Gaunidinium chloride: Dispose of via the normal chemical waste route with
high levels of dilution.
Chloroform: COLLECT ALL WASTE SOLVENT AND DISPOSE OF VIA THE HALOGENATED WASTE SOLVENT
ROUTE.
12
REVIEW
Enter the date or circumstances for review of assessment (maximum review interval 5 years)
Isoamyl alcohol. Chloroform; Tween 80; 2-mercaptoethanol; Gaunidinium chloride: april 2007
13
EMERGENCY ACTION
TO CONTROL HAZARDS To stabilize situation eg spread absorbant on liquid spill; eliminate sources of ignition, etc.
Gaunidinium chloride: WEAR CHEMICAL SAFETY GOGGLES, WEAR RUBBER GLOVES. SWEEP UP, PLACE IN A
BAG AND HOLD FOR WASTE DISPOSAL. AVOID RAISING DUST. VENTILATE AREA AND WASH SPILL SITE
AFTER MATERIAL PICKUP IS COMPLETE. EVACUATE AREA.
2-mercaptoethanol: WEAR CHEMICAL SAFETY GOGGLES, WEAR RUBBER GLOVES. USE ABSORBANT. SWEEP
UP, PLACE IN A BAG AND HOLD FOR WASTE DISPOSAL. AVOID RAISING DUST. VENTILATE AREA AND
WASH SPILL SITE AFTER MATERIAL PICKUP IS COMPLETE. EVACUATE AREA.
Tween 80. WEAR CHEMICAL SAFETY GOGGLES, WEAR GLOVES. ABSORB ON SAND OR VERMICULITE AND
PLACE IN CLOSED CONTAINERS FOR DISPOSAL. VENTILATE AREA AND WASH SPILL SITE AFTER
MATERIAL PICKUP IS COMPLETE.
Isoamyl alcohol; Chloroform. WEAR CHEMICAL SAFETY GOGGLES, WEAR GLOVES. WEAR DISPOSABLE
COVERALLS AND DISCARD THEM AFTER USE. ABSORB ON SAND OR VERMICULITE AND PLACE IN
CLOSED CONTAINERS FOR DISPOSAL. VENTILATE AREA AND WASH SPILL SITE AFTER MATERIAL PICKUP
IS COMPLETE. EVACUATE AREA.
TO PROTECT PERSONNEL Evacuation, protection for personnel involved in clean-up, Special First Aid
Isoamyl alcohol; Chloroform; Tween 80; 2-mercaptoethanol; Gaunidinium chloride: Evacuate, Isolate the area and inform
personnel.
Clean-up/decontamination
TO RENDER SITE OF EMERGENCY SAFE
Isoamyl alcohol; Chloroform; 2-mercaptoethanol; Gaunidinium chloride: VENTILATE AREA AND WASH SPILL SITE
AFTER MATERIAL PICKUP IS COMPLETE. EVACUATE AREA.
CONTACT
G S BESRA
PHONE
58123
10.10.00
*
**
***
Prefix T is used for Teaching Assessment Number.
Please include amount of chemicals used and how.
Hazdat No is the UNICOSHH datasheet report number.
Biosciences No is the Biosciences data sheet number.
UNICOSHH IS A CHEMICAL DATABASE ON THE HEALTH AND SAFETY UNIT SERVER.
BIOSCIENCES DATA SHEETS ARE AVAILABLE IN THE SCHOOL SAFETY OFFICE.