UCSF Lab Standard Operating Procedure
Chemical Process, Name or Hazard Class:
(Trimethylsilyl)diazomethane (TMSD)
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☒Hazardous Chemical
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Purpose
(Trimethylsilyl)diazomethane (TMSD) is a flammable liquid that is very toxic when inhaled. Inhalation can
cause pulmonary edema. It may be harmful if ingested or absorbed through the skin. It causes respiratory
tract, skin, and eye irritation. Symptoms may be delayed for hours. It is suspected to cause cancer and damage
to fertility. (Trimethylsilyl)diazomethane is mainly used as a methylating agent for generating methyl esters
from carboxylic acids. It is regarded as a less explosive alternative to diazomethane, but it should still be
handled with care. Two reports of exposure to TMSD resulted in death, both from respiratory distress (as of
2011).
Physical & Chemical Properties/Definition of Chemical Group
CAS#: 18107-18-1
Class: Flammable, carcinogen, reproductive hazard, very toxic, irritant
Molecular Formula: C4H10N2Si
Form (physical state): Liquid
Color: Yellow, greenish yellow
Boiling point: 94-96 °C
(Trimethylsilyl)diazomethane (TMSD)
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Date: Click here to enter a date.
Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/)
Potential Hazards/Toxicity
(Trimethylsilyl)diazomethane is a flammable liquid and vapor that is very toxic or fatal when inhaled.
Inhalation may cause pulmonary edema, drowsiness, and dizziness. It may be harmful if ingested or absorbed
through the skin. Symptoms from exposure include gastrointestinal discomfort, central nervous system
depression, lung irritation, chest pain, giddiness, slowed reaction time, slurred speech, headache, dizziness,
drowsiness, unconsciousness, nausea, vomiting, and diarrhea. It causes respiratory tract, skin, and eye
irritation. Symptoms may be delayed. Prolonged skin exposure may cause defatting and dermatitis. Contact
with eyes can cause redness, tearing, and blurred vision. May cause adverse liver effects. May cause cancer.
Suspected of damaging fertility.
Engineering Controls
Use only in a closed system or a certified chemical fume hood with effective ventilation. Electrically grounded
lines and equipment.
Personal Protective Equipment (PPE)
Respirator Protection
If lab personnel would like to use respirator on a voluntary basis, they must be trained and fit-tested by
EH&S. This is a regulatory requirement. (http://or.ucsf.edu/ehs/8193-DSY/version/default/part/4/data/)
Hand Protection
Handle with gloves. Nitrile gloves are recommended.
NOTE: Consult with your preferred glove manufacturer to ensure that the gloves you plan on using are
compatible with (trimethylsilyl)diazomethane.
Refer to glove selection chart from the links below:
http://www.ansellpro.com/download/Ansell_8thEditionChemicalResistanceGuide.pdf
OR
http://www.allsafetyproducts.biz/page/74172
OR
http://www.showabestglove.com/site/default.aspx
OR
http://www.mapaglove.com/
Eye Protection
ANSI approved, tight-fitting glasses/goggles. Face shields are recommended.
Skin and Body Protection
Flame-resistant/cotton lab coat preferably made of anti-static material, long pants, closed-toe shoes.
Hygiene Measures
Avoid contact with skin, eyes, and clothing. Wash hands before breaks and after handling.
First Aid Procedures
If inhaled
Move person into fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen.
Immediate medical attention is required.
(Trimethylsilyl)diazomethane (TMSD)
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Date: Click here to enter a date.
Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/)
In case of skin contact
Flush with plenty of water for at least 15 minutes while removing contaminated clothing. Take victim
immediately to hospital.
In case of eye contact
Flush eyes with plenty of water for at least 15 minutes lifting upper and lower eyelids and removing contact
lenses. Consult a physician.
If swallowed
Do not induce vomiting. Never give anything by mouth to an unconscious person. Rinse mouth with water.
Consult a physician.
Special Handling and Storage Requirements
Precautions for safe handling: Avoid contact with skin, eyes, and clothing. Avoid inhalation and ingestion.
Use explosion-proof equipment. Keep away from sources of ignition- No smoking. Prevent the build-up or
electrostatic charge.
Conditions for safe storage: Keep container tightly closed in a cool, dry, and well-ventilated area. Opened
containers must be carefully resealed and kept upright to prevent leakage. Avoid temperature extremes and
direct sunlight. Store in flammables area. Avoid oxidizing agents, chlorine, fluorine, and perchlorates.
Special Handling and Storage Requirements
Precautions for safe handling
Avoid contact with skin and eyes. Avoid inhalation of vapor or mist. Keep away from sources of
ignition (such as Bunsen burners). Take measures to prevent the build-up of electrostatic charge.
Conditions for safe storage
Keep container tightly closed in a dry and well-ventilated place. Containers which are opened must
be carefully resealed and kept upright to prevent leakage.
Possibility of hazardous reactions
Vapors may form explosive mixture with air.
Conditions to avoid
Heat, flames and sparks. Extremes of temperature and direct sunlight.
Materials to avoid
Oxygen, Oxidizing agents, Halogens, Reducing agents, Perchlorates, Trimethylaluminum
Hazardous decomposition products
Hazardous decomposition products formed under fire conditions. - Carbon oxides
Spill and Accident Procedure
Chemical Spill Dial 9-911 from campus phone or 415-476-1414 from cell phone or 415-2068522 (SFGH only)
(Trimethylsilyl)diazomethane (TMSD)
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Date: Click here to enter a date.
Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/)
Spill – Assess the extent of danger. Assist contaminated or injured persons. Evacuate the spill area.
Avoid breathing vapors. If possible, confine the spill to a small area using a spill kit or absorbent
material. Keep others from entering contaminated area (e.g., use caution tape, barriers, etc.).
Small (<1 L) – If you have training, you may assist in the clean-up effort. Use appropriate personal
protective equipment and clean-up material for chemical spilled. Double bag spill waste in clear
plastic bags, label and take to the next chemical waste pick-up.
Large (>1 L) – Dial 9-911 from campus phone or 415-476-1414 from cell phone or 415-2068522 (SFGH only) for assistance.
Chemical Spill on Body or Clothes – Remove clothing and rinse body thoroughly in emergency
shower for at least 15 minutes. If discomfort persists, proceed to the Emergency Department. If no
further discomfort is experienced, have the SDS ready and contact Poison Control Hotline at 1-800222-1222 for further exposure information. Notify your direct supervisor and EH&S at 415-4761300 during work hours, or 9-911 during non-working hours and weekends.
Chemical Splash Into Eyes – Immediately rinse eyeball and inner surface of eyelid with water for
15 minutes by forcibly holding the eye open. If discomfort persists, proceed to the Emergency
Department. If no further discomfort is experienced, have the SDS ready and contact Poison
Control Hotline at 1-800-222-1222 for further exposure information. Notify your direct
supervisor and EH&S at 415-476-1300 during work hours, or 9-911 during non-working
hours and weekends.
Medical Emergency Dial 9-911 (campus phone) or 476-6911 (cell phone)
Note: All serious injuries must be reported to EH&S at 415-476-1300 within 8 hours.
Non-Life Threatening Emergency– Go to Occupational Health Programs (OHP) Clinic, 415-8857580, 2330 Post Street, Suite 460 Hours of Operation for Appointments: Monday - Friday
7:30 a.m. - 4:00 p.m. (except Holidays).
Note: All serious injuries must be reported to EH&S at 415-476-1300 within 8 hours.
Needle stick/puncture exposure (as applicable to chemical handling procedure) – Wash the
affected area with antiseptic soap and warm water for 15 minutes. For mucous membrane
exposure, flush the affected area for 15 minutes using an eyewash station. Page the needle stick
nurse by dialing 415-353-7842 (STIC).
Decontamination/Waste Disposal Procedure
Clean contaminated surfaces with soap and water and paper towels. Dispose of the paper towels as
hazardous waste.
Safety Data Sheet (SDS) Location
(Trimethylsilyl)diazomethane (TMSD)
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Date: Click here to enter a date.
Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/)
Online SDS can be accessed at http://or.ucsf.edu/ehs/7241-DSY/msds.html
Protocol/Procedure
Quantities covered by this SOP:
______ (g , ml) to _______ (g, ml)
Temperature range covered by this SOP:
__ °C – __ °C
General Overview and Purpose:
Enter the experimental purpose
Procedure:
Enter experimental procedure. You can copy procedure from your lab notebook or from literature.
NOTE
Any deviation from this SOP requires approval from the Principal Investigator.
(Trimethylsilyl)diazomethane (TMSD)
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Date: Click here to enter a date.
Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/)