Lecture of Clinical Microscopy 40431300 Credit:1 Instructor: Ya-Chien Yang The course is an essential curriculum offered to the juniors of School of Medical Technology in the first semester. The students have to pass this course before entering the practice course of Clinical Microscopy. Course syllabus This course provides an overview of clinical utilities, principles and techniques of the clinical microscopy. The course will cover the examinations of urine, stool, sputum, cerebrospinal fluid, ascites and pleural effusion, synovial fluid, semen, gastric juice and duodenal juice, amniotic fluid, pregnancy test, quality assurance in clinical microscopy laboratory. The class will also include basic concepts of clinical physiology, such as EKG and pulmonary function tests. In addition, specimen collection for microbiological examination and the basics of the optical microscope will also be introduced. Laboratory of Clinical Microscopy 40431400 Credit:1 Instructor: Ya-Chien Yang The course is an essential curriculum offered to the juniors of School of Medical Technology in the first semester. The students have to pass this course before entering the practice course of Clinical Microscopy. Course syllabus This laboratory course is designed to be a procedural-orientated course coordinating the lecture course of clinical microscopy. Students will be assigned to practice the examinations of urine, stool, sputum, cerebrospinal fluid, ascites and pleural effusion, and pregnancy test as performed in the clinical laboratory. Students will also practice EKG and pulmonary function tests by using simple instruments. 血液學 (Hematology) 40436300 林亮音、胡忠怡、林淑華 The course (Hematology) is offered to the third year students of our department and is arranged every Monday afternoon from 3 to 5 at the first semester. This course introduces normal physiology function of red cells (hematopoiesis, hemoglobin, metabolism, nutrition, membrane, oxygen carrier and carbon dioxide carrier), white cells (lymphocyte, neutrophil, monocyte, basophil, eosinophil), platelets and coagulation (coagulation factors, coagulation, and fibrinolysis). 醫事技術學系 血液學實驗 (Hematology Experiment) 40436400 林亮音、胡忠怡、林淑華 The course (Hematology Experimant) is offered to the third year students who have passed the Hematololgy course, it is coupled with Clinical Hematology course, and it is arranged every Friday afternoon from 1 to 5 at the second semester. In this course, the students will be trained to familiar to routine hematology experiments at both principle and practice aspects, such as macroscopic and microscopic observation of blood, blood counting, blood smear preparation and staining, WBC classification, special tests for various anemia, bleeding time, PT and aPTT. 醫事技術學系 臨床血液學 (Clinical Hematology) 46300 林亮音、胡忠怡、林淑華 The course is for seniors who have knowledge in hematology and have taken related courses, i.e., basic hematology and laboratory hematology. The course will cover the following areas: introduction to anemia, acute and chronic leukemia and lymphoma, multiple myoloma, defects in platelet structure and function, and in hemostatic function and thrombotic abnormalities. 醫事技術學系 血庫學 (Blood Banking) 40446600 林東燦、林亮音 The course (Blood Banking) is for seniors of Medical Technology students, and it is arranged every Tuesday morning from 9 to 10 at the first semester. This course covers the following areas: introduction and identification of various blood types, introduction and preparation of various blood components, various laboratory tests for proper transfusion and investigation of transfusion reaction. MT4 Instrument Analysis 1 credit Time: Intensive course before laboratory practices Room: D506 Topic Speakers UV, visible and IR spectrophotometer I & II JT Kao Nephelometry and Turbidimetry RF Hsieh Scintillation counter RF Hsieh Flame photometry WH Fang Atomic Absorption WH Fang Fluorometry and luminometry SB Lin Electrophoresis WH Fang Potentiometry and coulometry JT Kao Centrifugation WH Fang Flow cytometry RF Hsieh Automatic blood cell counter CY Hu High performance liquid chromatography SB Lin Gas chromatography SJ Liao Microscopy LJ Teng Practice of Clinical Microscopy 40441400 Credits:3 Instructor: Ya-Chien Yang The course is an essential curriculum offered to the seniors of School of Medical Technology in the both semesters. The students have to pass the lecture and practice courses of Clinical Microscopy before entering the practice course. Each small group of 4 to 5 students will practice in the Division of Clinical Microscopy, Laboratory Medicine Department, NTUH, from Monday to Friday (8:30 to 17:00) for three weeks. Course syllabus This course is designed to provide a clinical laboratory education. Students will participate in clinical practicum experiences such as the examinations of urine, stool, sputum, cerebrospinal fluid, ascites and pleural effusion, synovial fluid, and pregnancy test in the diagnostic laboratory. Students will also get the supervised phlebotomy experience including routine venipuncture and specimen handling. Clinical Bacteriology and Mycology 404 44810 Credits:2 Instructors: Shen-Wu Ho, Lee-Jene Teng, Shwu-Jen Liaw, Hsin-Chih Lai This course discusses basic principles and concepts of diagnostic bacteriology and mycology. It includes two major parts, (I) identification systems and antimicrobial susceptibility and (II) laboratory diagnosis of infectious diseases at various body sites. In the identification section, the course covers organisms by taxonomic groups and concentrates on laboratory identification of significant isolates by conventional methods, rapid tests or new techniques such as molecular approaches. The references include English textbooks and recent papers. 醫技系 臨床細菌及黴菌學實驗 40444910 賴信志、何憲武、鄧麗珍、廖淑貞 (HC Lai)This course is designed for the fourth year students in this Department. The whole period takes one year. Students are grouped based on practical situation for efficient teaching purpose. The contents of the course include: isolation and identification of common and clinically important bacteria and fungi; The principles and practical operational procedures are all emphasized. Students learn important techniques including making staining solution and culture media, and observation of characteristics of bacterial species. The specific pre-practice technical training is emphasized for the purpose of training high-quality National-class experts in the field of clinical diagnosis. 臨床血清免疫學實習 40445100 謝榮峰 This course is mainly in Laboratory thecniques setup teaching and how to applicate to clinical tests, and also learn how to do a real clinical work in the hospital 醫事技術學系 臨床血清免疫學 40445300 謝榮峰、楊雅倩、陶秘華 This course mainly makes a bridging course between basic immunology and clinical immunology, and mentioned about the applications on disease association, and also the application of laboratory techniques. 醫事技術學系 臨床血清免疫學實驗 40445400 謝榮峰 This course mainly in training of various kind of immunology basic technique and applications. 醫事技術學系 臨床血液學實習 (Clinical hematology practice) 404 46400 林亮音、胡忠怡、林淑華 The course (Clinical Hematology Practice) is offered to the fourth year students who have passed the Hematololgy, Clinical Hematology and Hematology Experiment courses, it is coupled with Clinical Hematology Experiment course, and it is arranged for three weeks. In this course, the students will be assigned to participate in the clinical activities in our Hospital, such as automatic cell counting practice, automatic differential counter practice, automatic coagulation tests practice, quality control, so as to understand the basic requirement be a technician at Hematology lab. In addition, they should be read recent hematology-associated article from updated high-grade journals and make presentation to class. 醫事技術學系 臨床血液學實驗 (Clinical hematology Experiment) 404 46420 林亮音、胡忠怡、林淑華 The course (Clinical Hematology Experimant) is offered to the fourth year students who have passed the Hematololgy, Clinical Hematology and Hematology Experiment courses, it is coupled with Clinical Hematology Practice course, and it is arranged for two weeks. In this course, the students will be trained to familiar to special hematology experiments in addition to routine tests, so as to be appropriate for the challenge in Clinical Hematology Practice at our Hospital, such as hemoglobin electrophoresis, lab tests for hemolytic anemias, cytochemical stains for leukemic cells, bone marrow observation and differential diagnosis of various bleeding diseases. 醫事技術學系 血庫實習 404 46500 林東燦、林亮音 Blood Banking Practice The course (Blood Banking Practice) is for seniors of Medical Technology students who have passed Blood Banking and it is arranged for one week. This course provides students understanding the basic laboratory technique about Blood Banking, such as: blood typing, direct and indirect Coombs’ test, crossmatching, irregular antibody screening and identification, preparation and storage of blood components. Moreover, these students could enter the clinical lab at our hospital for practice and quality control exercise. Furthermore, these students have the opportunities to Taipei Blood Center for understanding the complicated procedures from blood correction to blood components in detail at Friday afternoon. 醫事技術學系 臨床病毒學 40447500 高全良、李君男、張淑媛 The course was designed mainly for senior undergraduates, who have taken microbiology and immunology, of our Department. The course is one credit and is given every year in Fall semester. The aim of this course is to help students to understand the underlying principles and techniques of laboratory diagnosis in viral infectious disease. The first part of this course is the general introduction to the tissue/cell cultures, principle of virus culture/isolation, rapid diagnosis method, all kinds of antigen and antibody detection methods, such as complement fixation test, neutralization assay, hemagglutination assay, immunofluorescent assay, enzyme-linked immunosorbent assay, and western blotting, etc. The second part consists of in-depth discussions on each virus, which includes their unique properties, clinical symptoms and the related laboratory diagnosis methods. Adenovirus, herpes simplex virus, varicella zoster virus, cytomegalovirus, Epstein-Barr virus, influenza virus, parainfluenza virus, Mumps virus, respiratory syncytium virus, measles virus, rubella virus, dengue virus, Japanese encephalitis virus, enterovirus, rotavirus, human immunodeficiency virus, and hanta virus will be covered in this course. 醫事技術學系 臨床病毒學實習 404 47600 高全良、李君男、張淑媛 The course was designed mainly for senior undergraduates of our Department. Microbiology, immunology, clinical virology and clinical virology experiment are pre-requisites for this course. The course is one credit and is given eight times every year. For each round, students will practice in the Division of Clinical Virology, Laboratory Medicine Department, NTUH, from Tuesday to Friday (8:30 to 17:00) for two weeks. The course is aimed to help students to understand the overall process and the routine practices in the viral diagnostic laboratory; and is part of the training for a medical technologist. Students will learn the virus isolation, determination of anti-viral antibody, rapid diagnosis, and chlamydia diagnosis, etc. During the 2-week practice, students must identify the viral origin of a given unknown specimen using the tests in the viral diagnostic laboratory. In the second week, each student will give an oral presentation about one recent paper in related field and participate in a panel discussion. 醫事技術學系 臨床病毒學實驗 40447700 高全良、李君男、張淑媛 The course was designed mainly for senior undergraduates of our Department. Microbiology, immunology, and clinical virology are pre-requisites for this course. The course is one credit and is given four times every year. The aim of this course is to help students familiar with the basic techniques used in the viral diagnostic laboratory and research in the future. The course consists of cell culture (preparation of medium, primary culture and subculture), virus culture (cell culture and chicken embryo inoculation), titration of virus titer (TCID50 and plaque assay), complement fixation assay, hemagglutination assay, hemagglutination inhibition assay, hemadsorption assay, neutralization assay, Direct/indirect immunofluorescent assay, enzyme-linked immunosorbent assay, and polymerase chain reaction, etc. MT4 Practice in Clinical Biochemistry Time: 3 weeks rotation Room: Department of Laboratory Medicine, NTUH This course provide 3 weeks rotation for practicing in Clinical Biochemistry. All the routine performed analysis should be practiced. Currently the instruments used by Clinical Biochemistry laboratory include: General Biochemical assays: Toshiba 100, 200 Blood gas Dry Chemistry: Vitros 950 HPLC: VMA, catecholamine, HbA1c Atomic absorption: Copper and lead Isoenyzyme electrophoresis: CK, LD Osmometer Immunoassays: Axysm, TDx, IMX, Vitros eci, Immulite Manual items: 17 KS, ICG MT4 Clinical Chemistry Laboratory Time: 2 weeks rotation Room: Diognostic Building D302 This course provides basic laboratory technique training. Some topics are selected for practicing, including: Orientation, basic techniques, pipetting, reagent preparation, etc 1. Water preparation and determine purity of water. 2. Operation of spectrophotometer and wave length calibration. 3. Glucose determination, enzymatic assay, colorimetric assay, method evaluation, practice of quality control, interference by lipemia. (DM) 4. Total protein and albumin. 5. Calcium determination, interference by icteric samples. (electrolytes) 6. Creatinine determination, serum and urine, calculation for creatinine clearance test. (Renal function) 7. Creatine kinase determination. (Cardiac function) 8. Bilirubin determination, direct and indirect (liver function) 9. 17 KS determination. (hormone)