These safety training resources, prepared solely for the use of the Regents of the University of California, were provided by a variety of sources. It is your
responsibility to customize the information to match your specific operations. Neither the University of California nor any of its employees, makes any warranty,
express or implied, or assumes any legal liability or responsibility for the accuracy, completeness, or usefulness of any information, or represents that its use would
not infringe privately owned rights. Reference herein to any specific commercial product, process, or service by trade name, trademark, manufacturer, or otherwise,
does not necessarily constitute or imply its endorsement, recommendation, or favoring by the University of California. The views and opinions of authors expressed
herein do not necessarily state or reflect those of the University of California, and shall not be used for advertising or product endorsement purposes.
Chemical Use in Animal Research ● Standard Operating Procedures
BrDU/ENU: 5-bromo-2-deoxyuridine (BrDU, CAS#59-14-3)
Date SOP Created:
Instructions: Insert specific details pertaining to your research; contact EH&S or OLAC as needed for assistance.
Hazard information
Provide known hazards to humans and physical description, color, odor characteristics, etc. Example:
BrdU (Sigma, Cat. No. B5002, CAS no. 59-14-3) is a thymidine analog that gets
incorporated in DNA during replication. Thus it is used as an indicator of DNA synthesis
and replication. It is supplied in lyophilized form as a white powder with no odor. It is a
toxic and mutagenic substance which is harmful by inhalation, by contact with skin and
if swallowed. BrdU is a suspect human carcinogen and may cause eye irritation, birth
defects and heritable genetic effects. It is incompatible with strong oxidizing agents and
excessive heat. Symptoms of acute exposure include wheezing, coughing, shortness of
breath, burning in the mouth, throat, or chest.
Preparation
List procedures used to make the chemical solution. Be specific about the physical form (solid, liquid, etc.)
and locations for work (bench top, fume hood, biosafety cabinet), and be very specific about personal
protective equipment (PPE) to be worn when handling the material. Example: BrDU will be handled
only when appropriate personal protective equipment, including lab coat, closed-toed
shoes, nitrile exam gloves, and safety glasses or a faceshield, is worn. All solutions will
be prepared in the chemical fume hood in Room X. The amount of chemical to be used
will be weighed in Room X. The balance and surrounding areas will be cleaned
thoroughly when done. A diaper will be placed on the surface of the fume hood to
contain spills. All chemicals will be stored and labeled according to manufacturer’s
instruction.
Transportation
Discuss the precautions that will be taken if the chemical is to be transported; if all work will be done
within the same lab, this section is not applicable. Example: Solution for animals is made in Room
X. The solution is transported to the (add location) in a primary container that is leakproof and placed in a suitable package for transport to the animal room, along with a
clear hazard warning and contact information.
Use
List specifics regarding use of the chemical. Include information regarding PPE and location of work.
Example:
During use, post all work areas”Restricteed Area – BrdU in Use – Toxic/Mutagen”
1. Animal injections will be conducted only within a fume hood in Room X. The
animals will be injected intraperitoneally with a X mg/kg body weight dose of BrdU
(insert frequency).
2. The BrdU solution will be handled only when appropriate personal protective
equipment, including lab coat, closed-toed shoes, gloves, and safety glasses, is worn.
3. Following use, excess solutions will be transported back to the <<Insert PI
Name>> Laboratory (<<Insert Location>>) and disposed of in chemical waste
containers for pickup by EH&S.
Office of Environment, Health & Safety ● http://ehs.berkeley.edu/ ● 642-3073
OLAC Manager Initials/Date: _______________
Effective Dates: ____________ to ____________
These safety training resources, prepared solely for the use of the Regents of the University of California, were provided by a variety of sources. It is your
responsibility to customize the information to match your specific operations. Neither the University of California nor any of its employees, makes any warranty,
express or implied, or assumes any legal liability or responsibility for the accuracy, completeness, or usefulness of any information, or represents that its use would
not infringe privately owned rights. Reference herein to any specific commercial product, process, or service by trade name, trademark, manufacturer, or otherwise,
does not necessarily constitute or imply its endorsement, recommendation, or favoring by the University of California. The views and opinions of authors expressed
herein do not necessarily state or reflect those of the University of California, and shall not be used for advertising or product endorsement purposes.
Disposal
If unsure, contact EH&S
at 642-3073 to determine
if chemical disposal is
necessary for bedding,
unused drinking water,
and/or carcasses
following treatment.
Spill response and
emergency
procedures
Hazard
communication,
signs, cage cards,
etc.
Unique instructions
Additional
information or
references
Include language describing the proper method for disposing of unwanted waste. This waste may include
chemically contaminated materials from the preparation of solutions; in some cases bedding and waste
from the animal needs to be handled as hazardous waste. Example: No BRDU waste should go into
the normal trash. Dry chemical waste, including contaminated disposable laboratory
supplies such as gloves, absorbent toweling, weighing containers, pipette tips, etc., will
be separated and discarded into labeled, sealable bags within unbreakable plastic
containers which will be stored in Room X until EH&S pickup. Liquid waste, including
any unused BrDU solution will be collected in containers that can be securely closed for
disposal through EH&S. To arrange for waste pickup by EH&S, complete an electronic
Material Packing List (eMPL) form available at http://mpl.ehs.berkeley.edu/ and submit
it to EH&S. Make sure you follow the instructions when packaging the waste for pickup.
Improperly packaged waste will not be picked up. Carcasses and potentially
contaminated bedding must be red bagged within a biosafety cabinet, tied closed, and
deposited into a red barrel for disposal as biohazardous waste. After injections, needles
should be directly deposited into a sharps container without recapping.
Include a brief description of how an accidental spill will be handled. Example: Small amounts of
dilute chemical should be readily absorbed by a spill pillow, paper towels or granular
absorbent and immediately placed in a fume hood. Any waste generated should be
disposed of through EH&S as chemical waste, see disposal section. A large spill of
concentrated chemical can create an irritating and potentially dangerous level of
airborne vapor. Absorbent material should be placed over the spill if possible and the
lab should be evacuated of all personnel if necessary. For emergency clean up
response, notify EH&S at 642-3073. In the case of exposure, rinse eyes or exposed skin
for at least 15 minutes, get fresh air and seek immediate medical attention at the Tang
Center’s Urgent Care Clinic on Bancroft (after hours call 643-7197 or go to Alta Bates
Hospital on Ashby)
All researchers handling this material should read and sign this document. When
animals have been treated with/administered this chemical, all cage cards should be
labeled “Brdu – Toxic/Mutagen. Researcher will change cage.” with the date of
injection and this SOP should be posted in a plastic sleeve on the applicable cage rack
or housing door to notify OLAC staff and other personnel. Also have the OLAC manager
review and initial below prior to posting.
BRDU must never be disposed of down the drain. All waste containing BRDU must be
collected as hazardous waste and disposed via EH&S. Because BRDU may be excreted
by animals, researchers must tag cages “Treated with BRDU - Do not change cage”and
researchers are responsible for changing the first time after treatment (e.g. after one
week) within a biosafety cabinet and bagging waste bedding for disposal as
biohazardous waste. If there is no further treatment with Brdu, Brdu signage may be
removed and OLAC may resume normal cage changing. If you have questions, ask the
OLAC manager, a vet or EH&S for clarification.
Refer to the MSDS available at http://www.ehs.berkeley.edu/healthsafety/msds.html, your
lab’s chemical hygiene plan, your MAUP, or contact your supervisor or EH&S at 6423073 for further guidance.
Office of Environment, Health & Safety ● http://ehs.berkeley.edu/ ● 642-3073
OLAC Manager Initials/Date: _______________
Effective Dates: ____________ to ____________
These safety training resources, prepared solely for the use of the Regents of the University of California, were provided by a variety of sources. It is your
responsibility to customize the information to match your specific operations. Neither the University of California nor any of its employees, makes any warranty,
express or implied, or assumes any legal liability or responsibility for the accuracy, completeness, or usefulness of any information, or represents that its use would
not infringe privately owned rights. Reference herein to any specific commercial product, process, or service by trade name, trademark, manufacturer, or otherwise,
does not necessarily constitute or imply its endorsement, recommendation, or favoring by the University of California. The views and opinions of authors expressed
herein do not necessarily state or reflect those of the University of California, and shall not be used for advertising or product endorsement purposes.
Training documentation (name and date):
_________________________________________
_________________________________________
_________________________________________
_________________________________________
_________________________________________
_________________________________________
_________________________________________
_________________________________________
_________________________________________
_________________________________________
Office of Environment, Health & Safety ● http://ehs.berkeley.edu/ ● 642-3073
OLAC Manager Initials/Date: _______________
Effective Dates: ____________ to ____________