UCSF Lab Standard Operating Procedure
Chemical Process, Name or Hazard Class:
o-Toluidine
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Department:
Date SOP was written:
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This lab specific SOP has been reviewed and approved by:
Principal Investigator Name:
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Principal Investigator Signature
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Type of SOP:
☐ Process
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☒Hazardous Chemical
☐ Hazardous Class
Purpose
o-Toluidine is primarily used in the manufacture of azo dyes. It has been used in the laboratory as a research
chemical. If inhaled or absorbed through the skin it can affect the blood’s ability to carry oxygen. It is
carcinogenic and toxic.
Physical & Chemical Properties/Definition of Chemical Group
CAS#: 95-53-4
Class: Suspect Carcinogens, Toxic
Molecular Formula: C7H9N
Form (physical state): liquid
Color: clear to light yellow
Boiling point: 199 oC
Potential Hazards/Toxicity
Potential Acute Health Effects:
o-Toluidine
1
Date: Click here to enter a date.
Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/)
Very hazardous in case of skin contact (permeator), of ingestion, of inhalation. Hazardous in case of skin
contact (irritant), of eye contact (irritant). Potential Chronic Health Effects: Very hazardous in case of eye
contact (irritant). CARCINOGENIC EFFECTS: Classified + (PROVEN) by OSHA. Classified 2B (Possible for
human.) by IARC. Classified A2 (Suspected for human.) by ACGIH, 2 (Reasonably anticipated.) by NTP.
MUTAGENIC EFFECTS: Classified SUSPECTED for human. TERATOGENIC EFFECTS: Not available.
DEVELOPMENTAL TOXICITY: PROVEN The substance is toxic to blood, kidneys, the nervous system, the
reproductive system, liver, mucous membranes. Repeated or prolonged exposure to the substance can
produce target organs damage.
Engineering Controls
Work with this chemical in a certified ducted fume hood. Facilities storing or utilizing this chemical should be
equipped with an eyewash facility and a safety shower.
Personal Protective Equipment (PPE)
Respirator Protection
If lab personnel would like to use respirator on a voluntary basis, they must be trained and fit-tested by
EH&S. This is a regulatory requirement. (http://or.ucsf.edu/ehs/8193-DSY/version/default/part/4/data/)
Hand Protection
Handle with nitrile gloves. Gloves must be inspected prior to use. Use proper glove removal technique
(without touching
glove's outer surface) to avoid skin contact with this product. Dispose of contaminated gloves after use in
accordance with applicable laws and good laboratory practices. Wash and dry hands..
NOTE: Consult with your preferred glove manufacturer to ensure that the gloves you plan on using are
compatible with o-toluidine
Refer to glove selection chart from the links below:
http://www.ansellpro.com/download/Ansell_8thEditionChemicalResistanceGuide.pdf
OR
http://www.allsafetyproducts.biz/page/74172
OR
http://www.showabestglove.com/site/default.aspx
OR
http://www.mapaglove.com/
Eye Protection
ANSI approved safety glasses or goggles
Skin and Body Protection
Long pants, closed toed shoes, and laboratory coat
Hygiene Measures
Avoid contact with, skin, eyes, and clothing. Handle in accordance with good industrial hygiene and safety
practice. Wash hands before breaks and immediately after handling the chemical.
First Aid Procedures
If inhaled
o-Toluidine
2
Date: Click here to enter a date.
Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/)
If inhaled, remove to fresh air. If not breathing, give artificial respiration. If breathing is difficult consult a
physician.
In case of skin contact
After contact with skin, wash immediately with plenty of water. Gently and thoroughly wash the
contaminated skin with running water and non-abrasive soap. Be particularly careful to clean folds, crevices,
creases and groin. Cold water may be used. Cover the irritated skin with an emollient. If irritation persists,
seek medical attention.
In case of eye contact
Check for and remove any contact lenses. In case of contact, immediately flush eyes with plenty of water for at
least 15
minutes. Cold water may be used. Get medical attention immediately.
If swallowed
Do not induce vomiting. Loosen tight clothing such as a collar, tie, belt or waistband. If the victim is not
breathing, perform mouth-to-mouth resuscitation. Seek immediate medical attention.
Special Handling and Storage Requirements
Potential Acute Health Effects: Very hazardous in case of skin contact (permeator), of ingestion, of
inhalation. Hazardous in case of skin contact (irritant), of eye contact (irritant). Potential Chronic Health
Effects: Very hazardous in case of eye contact (irritant). CARCINOGENIC EFFECTS: Classified + (PROVEN) by
OSHA. Classified 2B (Possible for human.) by IARC. Classified A2 (Suspected for human.) by ACGIH, 2
(Reasonably anticipated.) by NTP. MUTAGENIC EFFECTS: Classified SUSPECTED for human. TERATOGENIC
EFFECTS: Not available. DEVELOPMENTAL TOXICITY: PROVEN The substance is toxic to blood, kidneys, the
nervous system, the reproductive system, liver, mucous membranes. Repeated or prolonged exposure to the
substance can produce target organs damage.
Precautions for safe handling Avoid contact with skin and eyes. Avoid inhalation of vapour or mist.
Conditions for safe storage Keep container tightly closed in a dry and well-ventilated place. Containers
which are opened must be carefully resealed and kept upright to prevent leakage. Light sensitive. Store under
inert gas. Place in a secondary container. Recommended storage temperature 2-8 oC. Label the chemical, the
secondary container, and storage cabinet with a Cancer Hazard label..
Spill and Accident Procedure
Chemical Spill Dial 9-911 from campus phone or 415-476-1414 from cell phone or 415-2068522 (SFGH only)
Spill – Assess the extent of danger. Assist contaminated or injured persons. Evacuate the spill area.
Avoid breathing vapors. If possible, confine the spill to a small area using a spill kit or absorbent
material. Keep others from entering contaminated area (e.g., use caution tape, barriers, etc.).
Small (<1 L) – If you have training, you may assist in the clean-up effort. Use appropriate personal
protective equipment and clean-up material for chemical spilled. Double bag spill waste in clear
plastic bags, label and take to the next chemical waste pick-up.
Large (>1 L) – Dial 9-911 from campus phone or 415-476-1414 from cell phone or 415-2068522 (SFGH only) for assistance.
o-Toluidine
3
Date: Click here to enter a date.
Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/)
Chemical Spill on Body or Clothes – Remove clothing and rinse body thoroughly in emergency
shower for at least 15 minutes. If discomfort persists, proceed to the Emergency Department. If no
further discomfort is experienced, have the SDS ready and contact Poison Control Hotline at 1-800222-1222 for further exposure information. Notify your direct supervisor and EH&S at 415-4761300 during work hours, or 9-911 during non-working hours and weekends.
Chemical Splash Into Eyes – Immediately rinse eyeball and inner surface of eyelid with water for
15 minutes by forcibly holding the eye open. If discomfort persists, proceed to the Emergency
Department. If no further discomfort is experienced, have the SDS ready and contact Poison
Control Hotline at 1-800-222-1222 for further exposure information. Notify your direct
supervisor and EH&S at 415-476-1300 during work hours, or 9-911 during non-working
hours and weekends.
Medical Emergency Dial 9-911 (campus phone) or 476-6911 (cell phone)
Note: All serious injuries must be reported to EH&S at 415-476-1300 within 8 hours.
Non-Life Threatening Emergency– Go to Occupational Health Programs (OHP) Clinic, 415-8857580, 2330 Post Street, Suite 460 Hours of Operation for Appointments: Monday - Friday
7:30 a.m. - 4:00 p.m. (except Holidays).
Note: All serious injuries must be reported to EH&S at 415-476-1300 within 8 hours.
Needle stick/puncture exposure (as applicable to chemical handling procedure) – Wash the
affected area with antiseptic soap and warm water for 15 minutes. For mucous membrane
exposure, flush the affected area for 15 minutes using an eyewash station. Page the needle stick
nurse by dialing 415-353-7842 (STIC).
Decontamination/Waste Disposal Procedure
Clean contaminated surfaces with soap and water and paper towels. Dispose of the paper towels as
hazardous waste.
Safety Data Sheet (SDS) Location
Online SDS can be accessed at http://or.ucsf.edu/ehs/7241-DSY/msds.html
Protocol/Procedure
Quantities covered by this SOP:
______ (g , ml) to _______ (g, ml)
Temperature range covered by this SOP:
__ °C – __ °C
General Overview and Purpose:
o-Toluidine
4
Date: Click here to enter a date.
Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/)
Enter the experimental purpose
Procedure:
Enter experimental procedure. You can copy procedure from your lab notebook or from literature.
NOTE
Any deviation from this SOP requires approval from the Principal Investigator.
o-Toluidine
5
Date: Click here to enter a date.
Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/)