The use of higher plants and fungi for bioremediation of sites


Common publications by members of COST Action 859

(at least 2 different institutes from 2 different countries)


Bontidean L, Mortari A, Leth S, Brown NL, Karlson U , Larsen MM, Vangronsveld J ,

Corbisier P, Csoregi E (2004) Biosensors for detection of mercury in contaminated soils.


Dasgan HY, Abak K, Cakmak I , Romheld V , Sensoy S (2004) Inheritance of tolerance to leaf iron deficiency chlorosis in tomato. EUPHYTICA 139 (1): 51-57

Drager DB, Desbrosses-Fonrouge AG, Krach C, Chardonnens AN, Meyer RC, Saumitou-

Laprade P , Kramer U (2004) Two genes encoding Arabidopsis halleri MTP1 metal transport proteins co-segregate with zinc tolerance and account for high MTP1 transcript levels. PLANT JOURNAL 39 (3): 425-439

Germaine, K, Keogh E, Borremans B, van der Lelie D , Barac T, Oeyen L, Vangronsveld J ,

Porteous Moore F, Moore E, Campbell C, Ryan D, Dowling DN (2004) Colonisation of poplar trees by gfp expressing bacterial endophytes. FEMS Microbiology Ecology 48, 109-


Glombitza S, Dubuis PH, Thulke O, Welzl G, Bovet L , Gotz M, Affenzeller M, Geist B,

Hehn A, Asnaghi C, Ernst D, Seidlitz HK, Gundlach H, Mayer KF, Martinoia E, Werck-

Reichhart D , Mauch F, Schaffner AR (2004) Crosstalk and differential response to abiotic and biotic stressors reflected at the transcriptional level of effector genes from secondary metabolism. PLANT MOLECULAR BIOLOGY 54: 817-835

Golan-Goldhirsh A , Barazani O, Nepovim A, Soudek P, Smrcek S , Dufkova L, Krenkova

S, Yrjala K , Schröder P , Vanek T (2004) Plant response to heavy metals and organic pollutants in cell culture and at whole plant level. Journal of Soil and Sediments 4, 133-140

Lux A

, Šottníková A, Opatrná J,

Greger M (2004) Differences in structure of adventitious roots in Salix clones with contrasting characteristics of Cd accumulation and sensitivity.

Physiologia Plantarum 120, 537-545

Nepovim A, Podlipna R, Soudek P, Schröder P , Vanek T (2004) Effects of heavy metals and nitroaromatic compounds on horseradish glutathione S-transferase and peroxidase.

Chemosphere 57, 1007-1015

Renella G , Mench M , Van der Lelie D , Pietramellara G, Ascher J, Ceccherini MT, Landi L,

Nannipieri P (2004) Hydrolase activity, microbial biomass and community structure in longterm Cd-contaminated soils. Soil Biology Biochemistry 36, 443-451

Seuntjens P , Nowack B, Schulin R (2004) Root-zone modeling of heavy metal uptake and leaching in the presence of organic ligands. PLANT AND SOIL 265 (1-2): 61-73

Vassilev A ,

Schwitzguébel JP

, Thewys T, van der Lelie D , Vangronsveld J (2004) The use of plants for remediation of metal contaminated soils. The Scientific World Journal 4, 9-34

Whiting SN, Reeves RD, Richards D, Johnson MS, Cooke JA, Malaisse F, Paton A, Smith

JAC , Angle JS, Chaney RL, Ginocchio R, Jaffre T, Johns R, McIntyre T, Purvis OW, Salt

DE, Schat H , Zhao FJ , Baker AJM (2004) Research priorities for conservation of metallophyte biodiversity and their potential for restoration and site remediation.



Zha HG, Jiang RF, Zhao FJ , Vooijs R, Schat H , Barker JHA , McGrath SP (2004) Cosegregation analysis of cadmium and zinc accumulation in Thlaspi caerulescens interecotypic crosses. New Phytologist 163, 299-312


Baranska M, Schulz H , Baranski R, Nothnagel T, Christensen LP (2005) In situ simultaneous analysis of polyacetylenes, carotenoids and polysaccharides in carrot roots.


 Brown S, Christensen B, Lombi E, McLaughlin M, McGrath S , Colpaert J, Vangronsveld J

(2005) An inter-laboratory study to test the ability of amendments to reduce the availability of

Cd, Pb, and Zn in situ. Environmental Pollution 138, 34-45

Comino E , Whiting SN, Neumann PM, Baker AJM (2005) Salt (NaCl) tolerance in the Ni hyperaccumulator Alyssum murale and the Zn hyperaccumulator Thlaspi caerulescens .

PLANT AND SOIL 270 (1-2): 91-99

Cuypers A , Koistinen KM, Kokko H,

Kärenlampi S

, Auriola S, Vangronsveld J (2005)

Copper stress affects several PR-10 proteins and two putative chloroplast-related proteins in bean ( Phaseolus vulgaris L.), Journal of Plant Physiology 162, 383-392

Desbrosses-Fonrouge AG, Voigt K, Schroder A, Arrivault S, Thomine S , Kramer U (2005)

Arabidopsis thaliana MTP1 is a Zn transporter in the vacuolar membrane which mediates Zn detoxification and drives leaf Zn accumulation. FEBS LETTERS 579 (19): 4165-4174

Edwards R , Del Buono D , Fordham M, Skipsey M, Brazier M, Dixon DP, Cummins I

(2005) Differential induction of glutathione transferases and glucosyltransferases in wheat, maize and Arabidopsis thaliana by herbicide safeners. Zeitschrift Naturforschung. Biosci

C . 60. 307-316

Kurumata M, Takahashi M, Sakamoto A, Ramos JL, Nepovim A, Vanek T , Hirata T,

Morikawa H (2005) Tolerance to, and uptake and degradation of 2,4,6-trinitrotoluene (TNT) are enhanced by the expression of a bacterial nitroreductase gene in Arabidpsis thaliana. Z.

Naturforsch. 60c, 272-278

 Lanquar V, Lelievre F, Bolte S, Hames C, Alcon C, Neumann D, Vansuyt G, Curie C,

Schroder A, Kramer U , Barbier-Brygoo H, Thomine S (2005) Mobilization of vacuolar iron by AtNRAMP3 and AtNRAMP4 is essential for seed germination on low iron. EMBO

JOURNAL 24 (23): 4041-4051

Marcacci S, Raveton M , Ravanel P,

Schwitzguébel JP

(2005) The possible role of hydroxylation in the detoxification of atrazine in mature vetiver ( Chrysopogon zizanioides

Nash) grown in hydroponics.

Zeitschrift für Naturforschung C

60(5-6), 427-434

Nepovim A, Hebner A, Soudek P, Gerth A , Thomas H, Smrcek S , Vanek T (2005)

Phytoremediation of TBT by selected helophytes. Chemosphere 60, 1454-1461

Quartacci MF, Baker AJM , Navari-Izzo F (2005) Nitriloacetate- and citric acid assisted phytoextraction of cadmium by Indian mustard ( Brassica juncea (L.) Czernj, Brassicaceae ).

Chemosphere 59, 1249-1255

Renella G , Mench M , Landi L, Nannipieri P (2005) Microbial activity and hydrolase synthesis in long-term Cd-contaminated soils. Soil BiologyBiochemistry 37, 133-139


Renella G , Mench M , Gelsomino A, Landi A, Nannipieri P (2005) Functional activity and microbial community structure in soils amended with bimetallic sludges. Soil Biology

Biochemistry 37, 1498-1506

 Vassilev A , Perez-Sanz A, Semane B, Carleer R, Vangronsveld J (2005) Cadmium accumulation and tolerance of two Salix genotypes hydroponically grown in presence of cadmium. Journal of Plant Nutrition 28(12), 2159-2177

Villacieros M, Whelan C, Mackova M, Molgaard J, Sanchez-Contreras M, Lloret J, Aguirre de Carcer D, Oruezabal RI, Bolanos L, Macek T , Karlson U , Dowling D , Martin M, Rivilla

R (2005) Polychlorinated biphenyl rhizoremediation by Pseudomonas fluorescens F113 derivatives, using a Sinorhizobium meliloti nod system to drive bph gene expression. Appl.

Environ. Microbiol.

71, 2687-2694

Wang YD, Stauffer C, Keller C , Greger M (2005) Changes in Hg fractionation in soil induced by willow. PLANT AND SOIL 275 (1-2): 67-75 AUG 2005


Cape JN, Forczek ST, Gullner G , Mena-Benitez G, Schröder P , Matucha M (2006)

Progress in understanding the sources, deposition and above-ground fate of trichloroacetic acid. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH 13 (4): 276-286

Craciun AR, Courbot M, Bourgis F, Salis P, Saumitou-Laprade P , Verbruggen N (2006)

Comparative cDNA-AFLP analysis of Cd-tolerant and -sensitive genotypes derived from crosses between the Cd hyperaccumulator Arabidopsis halleri and Arabidopsis lyrata ssp petraea.


Eker S, Ozturk L, Yazici A, Erenoglu B, Romheld V , Cakmak I (2006) Foliar-applied glyphosate substantially reduced uptake and transport of iron and manganese in sunflower


54 (26): 10019-10025

Geebelen W, Sappin-Didier V, Ruttens A , Carleer R, Yperman J, Bongue-Boma K, Mench

M , Van der Lelie N , Vangronsveld J (2006) Evaluation of cyclonic ash, commercial Nasilicates, lime and phosphoric acid for metal immobilisation purposes in contaminated soils in Flanders (Belgium). ENVIRONMENTAL POLLUTION 144 (1): 32-39

Grytsyuk N , Arapis G , Davydchuk V (2006) Root uptake of Cs-137 by natural and seminatural grasses as a function of texture and moisture of soils. JOURNAL OF


Grytsyuk N , Arapis G , Perepelyatnikova L, Ivanova T, Vynograds'ka V (2006) Heavy metals effects on forage elements accumulation in crops yield and estimation of plants as affected by soil. SCIENCE OF THE TOTAL ENVIRONMENT 354 (2-3): 224-231

Hammond JP, Bowen HC, White PJ, Mills V, Pyke KA, Baker AJM , Whiting SN, May ST,

Broadley MR (2006) A comparison of the Thlaspi caerulescens and Thlaspi arvense shoot transcriptomes.

NEW PHYTOLOGIST 170 (2): 239-260

Hernandez-Allica J, Garbisu C , Becerril JM , Barrutia O, Garcia-Plazaola JI, Zhao FJ ,

Mcgrath SP (2006) Synthesis of low molecular weight thiols in response to Cd exposure in

Thlaspi caerulescens . PLANT CELL AND ENVIRONMENT 29 (7): 1422-1429


Kumpiene J , Ore S, Renella G , Mench M , Lagerkvist A, Maurice C (2006) Assessment of zerovalent iron for stabilization of chromium, copper, and arsenic in soil.


Marcacci S, Raveton M , Ravanel P and

Schwitzguébel JP

(2006) Conjugation of atrazine in vetiver ( Chrysopogon zizanioides Nash) grown in hydroponics. Environmental and

Experimental Botany 56, 205-215.

Mench M , Renella G , Gelsomino A, Landi L, Nannipieri P (2006) Biochemical parameters and bacterial species richness in soils contaminated by sludge-borne metals and remediated with inorganic soil amendments. ENVIRONMENTAL POLLUTION 144 (1): 24-31

Mench M, Vangronsveld J, Beckx C, Ruttens A (2006) Progress in assisted natural remediation of an arsenic contaminated agricultural soil. ENVIRONMENTAL POLLUTION

144 (1): 51-61

Quartacci MF, Argilla A, Baker AJM and Navari-Izzo F (2006) Phytoextraction of metals from a multiply contaminated soil by Indian mustard. Chemosphere 63, 918-925.

Renella G , Egamberdiyeva D, Landi L, Mench M , Nannipieri P (2006) Microbial activity and hydrolase activities during decomposition of root exudates released by an artificial root surface in Cd-contaminated soils. SOIL BIOLOGY & BIOCHEMISTRY 38 (4): 702-708

Ruttens A , Mench M , Colpaert JV, Boisson J, Carleer R, Vangronsveld J (2006)

Phytostabilization of a metal contaminated sandy soil. I: Influence of compost and/or inorganic metal immobilizing soil amendments on phytotoxicity and plant availability of metals. ENVIRONMENTAL POLLUTION 144 (2): 524-532

Ruttens A , Colpaert JV, Mench M , Boisson J, Carleer R, Vangronsveld J (2006)

Phytostabilization of a metal contaminated sandy soil. II: Influence of compost and/or inorganic metal immobilizing soil amendments on metal leaching. ENVIRONMENTAL

POLLUTION 144 (2): 533-539

Sarret G , Harada E, Choi YE, Isaure MP, Geoffroy N, Fakra S, Marcus MA, Birschwilks M,

Clemens S , Manceau A (2006) Trichomes of tobacco excrete zinc as zinc-substituted calcium carbonate and other zinc-containing compounds. PLANT PHYSIOLOGY 141 (3):


 Schwitzguébel JP

, Meyer J, Kidd P (2006) Pesticides removal using plants: phytodegradation versus phytostimulation. Chapter 13 in: Focus in Biotechnology, Vol 9A,

Phytoremediation / Rhizomediation, Mackova M, Dowling D and Macek T, editors. Springer

Verlag, ISBN 1-4020-4952-8, pp. 179-198

Tolra R, Pongrac P , Poschenrieder C , Vogel-Mikus K, Regvar M , Barcelo J (2006)

Distinctive effects of cadmium on glucosinolate profiles in Cd hyperaccumulator Thlaspi praecox and non-hyperaccumulator Thlaspi arvense . PLANT AND SOIL 288 (1-2): 333-341

Tuomainen MH, Nunan N, Lehesranta SJ, Tervahauta AI , Hassinen VH, Schat H ,

Koistinen KM, Auriola S, McNicol J, Karenlampi SO (2006) Multivariate analysis of protein profiles of metal hyperaccumulator Thlaspi caerulescens accessions. PROTEOMICS

6 (12): 3696-3706



Broadley MR , White PJ, Hammond JP, Zelko I, Lux A (2007) Zinc in plants. NEW

PHYTOLOGIST 173 (4): 677-702

Courbot M, Willems G, Motte P, Arvidsson S, Roosens N, Saumitou-Laprade P ,

Verbruggen N (2007) A major quantitative trait locus for cadmium tolerance in Arabidopsis halleri colocalizes with HMA4, a gene encoding a heavy metal ATPase. PLANT

PHYSIOLOGY 144 (2): 1052-1065

 de Carcer DA, Martin M, Mackova M, Macek T , Karlson U , Rivilla R (2007) The introduction of genetically modified microorganisms designed for rhizoremediation induces changes on native bacteria in the rhizosphere but not in the surrounding soil. ISME

JOURNAL 1 (3): 215-223

Gorinova N , Nedkovska M , Todorovska E, Simova-Stoilova L, Stoyanova Z, Georgieva K,

Demirevska-Kepova K, Atanassov A, Herzig R (2007) Improved phytoaccumulation of cadmium by genetically modified tobacco plants ( Nicotiana tabacum L.). Physiological and biochemical response of the transformants to cadmium toxicity. ENVIRONMENTAL

POLLUTION 145 (1): 161-170

Hassinen VH, Tervahauta AI , Halimaa P, Plessl M, Peraniemi S, Schat H , Aarts MGM ,

Servomaa K, Karenlampi SO (2007) Isolation of Zn-responsive genes from two accessions of the hyperaccumulator plant Thlaspi caerulescens . PLANTA 225 (4): 977-989

Lepp NW , Madejon P (2007) Cadmium and zinc in vegetation and litter of a voluntary woodland that has developed on contaminated sediment-derived soil. JOURNAL OF


Madejon P , Lepp NW (2007) Arsenic in soils and plants of woodland regenerated on an arsenic-contaminated substrate: A sustainable natural remediation? SCIENCE OF THE

TOTAL ENVIRONMENT 379 (2-3): 256-262

Madejon P , Murillo JM, Maranon T, Lepp NW (2007) Factors affecting accumulation of thallium and other trace elements in two wild Brassicaceae spontaneously growing on soils contaminated by tailings dam waste. CHEMOSPHERE 67 (1): 20-28

Perepelyatnikov G, Perepelyatnikova L, Ivanova T, Kalynenko L, Grytsyuk N , Zhurba M,

Davydchuk V, Arapis G (2007) Radiological efficiency of agricultural countermeasures applied in radiocontaminated fields. Journal of Environmental Engineering and Landscape

Management 15 (4): 243-252

Plaza S , Tearall KL, Zhao FJ , Buchner P, McGrath SP , Hawkesford MJ (2007) Expression and functional analysis of metal transporter genes in two contrasting ecotypes of the hyperaccumulator Thlaspi calerulescens . JOURNAL OF EXPERIMENTAL BOTANY 58 (7):


Pongrac P , Vogel-Mikus, Kump P, Necemer M, Tolra R, Poschenrieder C , Barcelo J,

Regvar M (2007) Changes in elemental uptake and arbuscular mycorrhizal colonisation during the life cycle of Thlaspi praecox Wulfen. Chemosphere 69: 1602-1609

Quartacci MF, Irtelli B, Baker AJM , Navari-Izzo F (2007) The use of NTA and EDDS for enhanced phytoextraction of metals from a multiply contaminated soil by Brassica carinata .

CHEMOSPHERE 68 (10): 1920-1928

 Schröder P

, Navarro-Avino J , Azaizeh H , Golan-Goldhirsh A , Di Gregorio S , Komives

T , Langergraber G , Lenz A, Maestri E , Memon AR , Ranalli A , Sebastiani L , Smrcek S ,


Vanek T , Vuilleumier S , Wissing F (2007) Using phytoremediation technologies to upgrade waste water treatment in Europe.


14 (7): 490-497

Semane B, Cuypers A , Smeets K, Van Belleghem F, Horemans N, Schat H , Vangronsveld

J (2007) Cadmium responses in Arabidopsis thaliana : glutathione metabolism and antioxidative defence system. PHYSIOLOGIA PLANTARUM 129 (3): 519-528

Shilev S , Fernandez Lopez A, Sancho Prieto M, Sancho ED (2007) Induced protein profile changes in arsenate tolerant and sensitive Pseudomonas fluorescens strains. Journal of

Environmental Engineering and Landscape Management 15 (4): 221-226

Shilev S , Naydenov, Tahsin N, Sancho ED , Benlloch M, Vancheva V, Sapundjieva K,

Kuzmanova J (2007) Effect of easily biodegradable amendments on heavy metal solubilization and accumulation in technical crops – A field trial. Journal of Environmental Engineering and

Landscape Management 15 (4): 237-242

Vanek T , Gerth A , Vakrikova Z, Podlipna R, Soudek P (2007) Phytoremediation of explosives.

Chapter 3.7 in: Advanced Science and Technology for Biological Decontamination of Sites

Affected by Chemical and Radiological Nuclear Agents, Marmiroli N, Samotokin B, Marmiroli

M, editors, NATO Science Series, IV. Earth and Environmental Sciences, Vol. 75, Springer,

ISBN 978-1-4020-5519-5, pp. 209-225

Vassillev A , Perez-Sanz A, Cuypers A , Vangronsveld J (2007) Tolerance of two hydroponically grown Salix genotypes to excess zinc. JOURNAL OF PLANT NUTRITION

30 (7-9): 1471-1482

Willems G, Drager DB, Courbot M, Gode C, Verbruggen N , Saumitou-Laprade P (2007)

The genetic basis of zinc tolerance in the metallophyte Arabidopsis halleri ssp halleri

(Brassicaceae): An analysis of quantitative trait loci. GENETICS 176 (1): 659-674


Arnetoli M, Vooijs R, ten Bookum W, Galardi F, Gonnelli C , Gabbrielli R , Schat H , Verkleij

J (2008) Arsenate tolerance in Silene paradoxa does not rely on phytochelatin-dependent sequestration. Environmental Pollution 152(3): 585-591

Arnetoli M, Vooijs R, Gonnelli C , Gabbrielli R , Verkleij J, Schat H (2008) High-level Zn and Cd rolerance in Silene paradoxa L. from a moderately Cd- and Zn-contaminated copper mine tailing. Environmental Pollution 156(2): 380-386

Bott S, Tesfamariam T, Candan H, Cakmak I , Roemheld V , Neumann G (2008)

Glyphosate-induced impairment of plant growth and micronutrient status in glyphosateresistant soybean ( Glycine max L.). Plant and Soil 312(1-2): 185-194

Clemente R , Dickinson NM , Lepp NW (2008) Mobility of metals and metalloids in a multi-element contaminated soil 20 years after cessation of the pollution source activity.

Environmental Pollution 155(2): 254-261

Koopmans GF, Romkens PFAM, Fokkema MJ, Song J, Luo YM, Japenga J , Zhao FJ

(2008) Feasibility of phytoextraction to remediate cadmium and zinc contaminated soils.

Environmental Pollution 156(3): 905-914

Mills RF, Doherty ML, Lopez-Marques RL, Weimar T, Dupree P, Palmgren MG , Pittman JK,

Williams LE (2008) ECA3, a Golgi-localized P-2A-type ATPase, plays a crucial role in manganese nutrition in Arabidopsis. Plant Physiology 146(1): 116-128


Ozturk L, Yazici A, Eker S, Gokmen O, Roemheld V , Cakmak I (2008) Glyphosate inhibition of ferric reductase activity in iron deficient sunflower roots. New Phytologist 177(4): 899-906

Palmgren MG , Clemens S , Williams LE , Krämer U , Borg S, Schjorring JK, Sanders D

(2008) Zinc biofortification of cereals: problems and solutions. Trends in Plant Science 13(9):


Pongrac P, Vogel-Mikus K, Regvar M , Tolra R, Poschenrieder C , Barcelo J (2008)

Glucosinolate profiles change during the life cycle and mycorrhizal colonization in a Cd/Zn hyperaccumulator Thlaspi praecox (Brassicaceae). Journal of Chemical Ecology 34(8): 1038-


Renella G , Landi L, Ascher J, Ceccherini MT, Pietramellara G, Mench M , Nannipieri P (2008)

Long-term effects of applied phytostabilisation of trace elements on microbial biomass and activity, enzyme activities, and composition of microbial community in the Jales contaminated mine spoils. Environmental Pollution 152(3): 702-712

Sas-Nowosielska A, Kucharski R, Pogrzeba M, Krzyzak J, Kuperberg JM, Japenga J (2008)

Phytoremediation technologies used to reduce environmental threat posed by metalcontaminated soils: theory and reality. In: Simulation and Assessment of Chemical Processes in a Multiphase Environment. NATO Science for Peace and Securiry Series C – Environmental

Security, 285-297

Schroeder P, Herzig R, Bojinov B, Ruttens A, Nehnevajova E , Stamatiadis S, Memon A ,

Vassilev A , Caviezel M, Vangronsveld J (2008) Bioenergy to save the world – Producing novel energy plants for growth on abandoned land. Environmental Science and Pollution

Research 15(3): 196-204

Wojas S , Clemens S , Hennig J, Skodowska A, Kopera E, Schat H , Bal W, Antosiewicz

DM (2008) Overexpression of phytochelatin synthase in tobacco: distinctive effects of

AtPCS1 and CePCS genes on plant response to cadmium. Journal of Experimental Botany

59(8): 2205-2219

Xing JP, Jiang RF, Ueno D, Ma JF, Schat H , McGrath SP , Zhao FJ (2008) Variation in root-to-shoot translocation of cadmium and zinc among different accessions of the hyperaccumulators Thlaspi caerulescens and Thlaspi praecox . New Phytologist 178: 315-325