LIUBOMIR ANDREI PISAROV http://www.pitt.edu/~pisarov 3259 Niaga Pittsburgh PA 15 Email: pisarov@pitt.edu Phone: 412.412 EDUCATION BS in Neuroscience, University of Pittsburgh, Pittsburgh PA PROFESSIONAL EXPERIENCE Senior Research Specialist, Department of Psychiatry University of Pittsburgh, Pittsburgh PA 2000 Assisted in a longitudinal study of prefrontal cortical functioning in heroin-dependent patients during the course of opiate maintenance using a newly developed variant of the GO/NO GO task, Delay Discount task and functional magnetic resonance imaging (fMRI). Performed femoral artery cannulations in rats undergoing chronic pharmacologic treatments. Research Specialist IV, Department of Pathology University of Pittsburgh, Pittsburgh PA 199 Performed immunohistochemical and laser confocal microscopic analysis of bloodbrain barrier tight junction proteins that are disrupted in HIV-1 encephalitis and immunohistochemical analysis of cell trafficking adhesion molecules. Grafted second trimester human fetal neuroglia into the ventricles of scid mice via stereotactic surgery and performed immunohistochemical analysis of the xenografts. Research Specialist III, Pittsburgh Cancer Institute University of Pittsburgh, Pittsburgh PA Used perfusion techniques to isolate human hepatocytes for cryopreservation, pharmaceutical studies, and cell transplantation in liver failure patients. Isolated human prostate epithelial cells and human liver, lung and intestinal microsomes. Extracted RNA from human organ tissues. Maintained inventory of all laboratory chemicals and reagents. Isolated, cultured and infected human hepatocytes with Hepatitis C. PUBLICATIONS Dallasta, L.M., Pisarov L. A., et al. “Blood-brain barrier tight junction disruption in human immunodeficiency virus-1 encephalitis.” American Journal of Pathology. 155(6):1915-27, 1999 Dec. O’Connell, J.F., S. Cox, et al. “Primary Human Hepatocyte Cultures for the Study of Hepatitis C Virus (HCV)”. Methods in Molecular Medicine, Totowa, NJ: Humanna Press. Chapter 55, 1998. 19 Runge, D.M. et al. "Epidermal Growth Factor - and Hepatocyte Growth Factor Receptor Activity in Serum-Free Cultures of Human Hepatocytes". Journal of Hepatology. 30:2, p. 265-74, 1999. Strom, S.C., L. A. Pisarov, et al. “The use of human hepatocytes to study P450 gene induction”. Methods of Enzymology 272, p. 388-401,1997. Strom, S.C., R. A. Fisher, et al. “Transplantation of Human Hepatocytes”. Transplant Proc., 29:4, p. 2103-6,1997. Summer Premedical Academic Enrichment Program (S.P.A.E.P.) 1990-1992, 8 weeks/sess Pittsburgh, PA Montefiore, Presbyterian, and Children’s Hospitals, 1992: assisted in surgery and post ope patient care for patients with total hip arthroplasty. Western Psychiatric Institute, 1991: assisted in patient interview, test administration, and p examination for clinical research concerning Alzheimer’s disease. Magee Women’s Hospital, 1990: assisted in fetal autopsy and placental processing, monit entered in Reflex Data Base System records of fetal and placental diseases for clinical stud AWARDS & HONORS University of Pittsburgh, Dean’s List, fall of 1992 and fall - summer of 1993 National Honor Society Westinghouse Science Honors Institute, 1989 LANGUAGES & SKILLS Oral and written fluency in Romanian and English PUBLICATIONS INTERNSHIPS