FORM A – APPLICATION TO REGISTER AS A STUD SIRE
Greyhound Racing SA
55 Cardigan St, Angle Park SA 5010
PO Box 2352, Regency Park SA 5942
Telephone: 08 8243 7100 Fax: 08 8268 2870
FEE : $155.00
PLEASE NOTE – Prior to submitting this application please ensure that the following is
observed:
* Fee is attached
* Semen evaluation is enclosed
* Application for DNA has been lodged with Greyhounds Australasia
_______________________________________________________________________________________
In consideration of Greyhound Racing SA granting this application to register the greyhound described in
the schedule herein, I agree to be bound by and to comply with all such rules in respect of registration as
shall for the time being and from time to time be made by the said Greyhound Racing SA, and all decisions
that shall from time to time be made thereunder by Greyhound Racing SA or by any other authority or
person authorised under such rules to make the same.
Date of application____________________Signature of
Applicant________________________
SCHEDULE
Name Of Greyhound
Whelped
Breeding: Sire Owner or Studmaster: Name Address:
Phone No.:
Colour
Certificate No.
Dam -
Ear Brand
Registration No.
NOTE: Where applicant is not owner or lessee of greyhound, an authority to register
services per Form B must be lodged with the office of Greyhound Racing SA.
I further agree that as a condition of registration and retention of registration that:
a) That before any registered greyhound is eligible to be used for Stud purposes, such greyhound must be DNA tested, be semen
evaluated and registered as a sire with Greyhound Racing SA. The application for stud registration shall be made by the
registered owner, owner-trainer of the sire or some person authorised thereto in writing by such owner or owner-trainer. The
application shall be made on the prescribed form to Greyhound Racing SA and shall be accompanied by the prescribed fee.
b) The number of collections taken from the greyhound which is the subject of this application shall not exceed 14 in any one
calendar month, regardless whether the collection is used for a natural mating or for freezing.
c) That I will accept responsibility for payment of any veterinary fees incurred by a direction of Greyhound Racing SA in relation to
fertility testing of the greyhound referred to in the schedule.
d) That if the tests referred to in the preceding clause (c) are not to the satisfaction of Greyhound Racing SA, then the said
Authority shall have the unqualified right to suspend the Stud registration for One (1) month and at the end of this period, I
shall submit the dog to further tests as required by Greyhound Racing SA.
e) That I clearly understand that Greyhound Racing SA shall have the right to publish the number of services by the dog referred to
herein and the number of litters produced from such services.
f)
That if the dog should fail to produce veterinary tests to the satisfaction of Greyhound Racing SA, then it shall be sufficient
reason for Greyhound Racing SA to suspend the dog’s Stud registration until it is satisfied that the dog can perform its stud
duties in a proper manner.
g) That I will ensure all services by the above mentioned sire are by natural means unless approval has been granted by the
Authority for any other service means.
h) That when registering this greyhound as a stud sire I will be bound by the Greyhound Racing Rules of Greyhound Racing SA.
______________________________________________________________________________________________________
_____
PAYMENT BY CREDIT CARD –
Card Number
Card Holder’s Name
If you would like to pay by credit card (Bankcard, Mastercard or Visa only)
please complete the following details
-
-
Signature
Expiry Date
/
EXPLANATORY NOTES FOR SEMEN EVALUATION BY VETERINARIAN/AUTHORISED
PERSON
COLLECTION
A bitch in season may or may not be available; to facilitate collection of the ejaculate but their
presence often helps considerably. Sires approved for A.I. are less likely to require such
motivation.
Collection should be made into a chemically clean, dry container, warmed to body temperature
(38-39°C). Protect the fresh semen from excess light, heat and cold.
Semen evaluation should be commenced as soon as possible following the collection.
MATERIAL REQUIRED
-
-
Chemically clean dry container.
Warming facility for collection container (eg. Microbiology incubator; container of warm
water; hot-air hair dryer; heat lamp).
Microscope glass slides and cover slips – also warmed to body temperature for motility
estimation.
Pasteur pipettes, or A.I. pipettes for transfer of semen from container to slides. Note
that the silicon lubricated plastic hypodermic syringes used for injections may be
spermicidal and reduce the number and motility of live sperm, leading to an inaccurate
evaluation.
UNOPETTE for Red Blood Cell Estimation No 5851 – this is used for direct sperm count
procedures.
Standard Neubauer haemocytometer with coverslip.
Microscope – to 400xhigh power.
EVALUATIONS
LIBIDO
-
Rate from one (poor) to three (good).
COLOUR OF SEMEN
-
Indicate findings of visual examination of fresh ejaculation.
MOTILITY
Using a warmed Pasteur pipette or A.I. pipette, transfer a
drop of fresh semen to a warmed glass slide. Apply a cover slip and
examine under
the microscope using 400xhigh power. Record
quality from 0-5 using the
following guidelines.
0
=
Dead
1
=
Slow side to side – no forward progression
2
=
Slow side to side – forward progression in spurts
3
=
Rapid side to side – forward progression in spurts
4
=
Slow forward progression
5
=
Fast forward progression
Now estimate percentage live to dead sperm and record.
SPERM COUNT PER ML – USING UNOPETTE NO5851 – (AS USED FOR RED BLOOD CEEL
ESTIMATION).
Open by inverting top and marking hole in container of fluid by downward push of cap. Fill capillary
pipette with fresh semen by touching capillary pipette to surface of ejaculate. Remove excess
semen from exterior of capillary pipette by wiping swiftly with a tissue. Squeeze Unopette container
to remove some air, insert capillary pipette firmly, and release pressure on container so that the
vacuum created draws the semen from the pipette into the container. Wash the solution up and
down the pipette 3-4 times by gently squeezing and releasing the sides of the container. Remove
the cap and squeeze it to empty the pipette into the container. Invert cap and push firmly into place
to seal the container.
Rotate Unopette for 45-60 seconds to ensure even mixing of contents – Do Not Shake.
Invert and squeeze Unopette to remove 5-6 drops of fluid then proceed immediately to fill the clean
dry haemocytometer chamber. If chamber overfills, repeat the process. Count the sperm in the four
(4) corner squares – then add four zeros (0000) to the total number of sperm counted in the five (5)
squares to give the total number of sperm per cubic millilitre.
ALTERNATIVELY – USING UNOPETTE NO.5855 - (AS USED FOR WHITE BLOOD CELL
ESTIMATIONS).
Count the sperm in the central area of small squares on both sides of the Neubauer
Hemocytometer, and divide the total by 2 to obtain the mean (average) of the two areas.
Multiply this figure by 1000 to obtain the number of sperm per cubic millilitre.
TOTAL SPERM COUNT
- equals sperm count per ml multiplied by volume of ejaculate in mls.
VOLUME OF EJACULATE
- as the motility assessment has already been completed, the volume measurement may be
undertaken using a measuring pipette, a graduated test tube, or a 5ml standard hypodermic
syringe. Record ejaculate volume to nearest 0.5ml.
GROSS AND DETAILED MORPHOLOGY
- return to the slide used for motility estimate, or, prepare a fresh similar slide. Count at least 200
sperm cells using 400xhigh power. Record normals and abnormals for each 100 cells counted.
Record also the R.B.C. and W.B.C. for each of 4 separate fields at 400x magnification and record
the average per field for each.
Record cellular debris seen over at least 4 separate fields as per R.B.C. and W.B.C.
VETERINARY/AUTHORISED PERSON ASSESSMENT
- a reasonable fertility rating should take into account a desirable requirement of 10 million sperm
per pup; so that for a litter of 10 pups one should look to 100 million live normal sperm per
ejaculate.
If the evaluation is unsatisfactory in any aspect, the procedure should be repeated in 48-96 hours
and/or appropriate therapy be instituted to correct any underlying clinical problem.
REFERENCE: Laboratory Procedures – Using The Unopette Brand System.; Benton & Dickson &
Co.
SEMEN EVALUATION REPORT
Name Of Sire_____________________________________Ear Brands__________________________
Colour________________________Date of Examination____________________________________
Studmaster_________________________________________________________________________
Address_____________________________________________________________________________
Phone___________________________Mobile_____________________________Facsimile________
Email______________________________________________________________________________
CLINICAL EVALUATION:
1.
Libido
Poor
Average
Good
2.
Colour Of Semen
Clear
Milky
Blood Tinged
3.
Motility
Poor
Average
Good
Excellent
Excellent
Percentage_____________________
4.
Volume Of Ejaculate_____________
5.
Sperm Count/Ml________________
6.
Total Sperm Count______________
7.
Gross Morphology
8.
Total Live Normal Cells At Time Of Ejaculate_____________
Normal______%
Abnormal______%
VETERINARY/AUTHORISED PERSON ASSESSMENT OF FERTILITY STATUS
I,_________________________________________ have assessed the semen for the greyhound as
detailed
Name
above, as satisfactory for the purpose of standing this greyhound at stud.
Signed______________________________________________________________________________
Veterinarian/Authorised Person
___________________________________________________________________________________
Address
Phone________________________Mobile______________________________Facsimile__________
Email______________________________________________________________________