1
Supplemental Table 4. Transplantation of Xenogeneic Hematopoietic Cells in
Immunotolerant Animals
Ref
Type
Cells
Host/
Radiation
Chimerism
of Blood
or BM
0.1-10% BMC
CD45+
Not examined
Identification of Bone
Marrow-derived
Hepatocytes
HuALB+; Huc-Met+
# of Host Hepatocytes
Evaluated
S43
hCB
S44
hCB/
TC
NODSCID M/
375 cGy
SCID M/
None
PHK26+; ALB+
Entire sections
scanned for PHK26+
cells
S45
hCB/
hBM
NODSCID or
NODSCID
β2M- M/ 300
cGy
NODSCID M/
250R
>0.5% periph.
blood cells
CD45+
ALB+CD45-
Not stated. Multiple
40x & 60x fields
scanned
S46
hCB
>64% BMC
CD45+
OCH1E5+/HepPar (Ab
putatively specific for
hHepatocytes) hDNA
sequences
~500,000
S47
hCB
Not examined
OCH1E5+; hALB+ PCR for
hX chromosome sequences
OCH1E5+; hALB+;
hCentromeric DNA
100 random fields, ?
mag.
Not quantified
hCB/
hPB
SCID M/
None
NODSCID
β2M-/ None,
but got 5-FU
NODSCID M/
350 cGy
S48
hCB
S49
hALB+; hα-1AT+; hDNA
sequences
Cells not quantified.
S50
hBM
(fetal)
NODSCID M/
300 cGy
Y+; hALB+CD45--
Not stated
S51
hCB
NODSCID M/
None
18% BMC
CD45+ in 7/10
hosts
Not examined
HuALB+; OCH1E5+; h- &
mX chromosome
sequences
3000 isolated; 40005000 in sections
S52
hCB
NODSCID M/
325 cGy
hAFP+; hALB+; OCH1E5+
Not stated.
S53
hPB/TC
NODSCID M/
None
1-6% BMC
expressed
human markers
Not examined
hALB+
Not quantified
S54
hCB
NODSCID M/
250 rad
0-32 % BMC
expressed
human markers
hALB+HepPar1+
Cells in 20 liver
sections
S55
hBM/
TC
Young adult
rats/
None, but
treated with
Cyclosporin A
Not examined
hAFP+; hALB+; hCK18+;
hAGPR+
Sections. Engrafted
cells estimated from
calculated volume of
foci.
S56
hCB/
hBM/
hPB
Preimmune
fetal sheep/
None
1.1-6.4% BMC
CD45+
OCH1E5+; hALB+; hDNA
sequences (ALU)
Not stated, but
involved 4 sections
from each of 4 lobes
S57
hCB
8-22% BMC
CD45+
hCB/
TC
OCH1E5+; hChromosome
8q22 sequence (lipoprotein
lipase gene)
OCH1E5+; hALB+
Not quantified.
S58
Preimmune
fetal sheep/
None
Preimmune
fetal sheep/
None
BMC 28%
CD34+, 42%
CD45+
Not examined
0.09-2.75%
BMC CD45+
Not stated
Not stated.
HEP-05-1202
% of Hepatocytes Putatively
Derived from
Transplanted Cells
0.05-0.1%. None expressed
both human & mouse proteins.
None found after direct
transplantation. 0.18 to 23
PHK26+ cells /section when
TC cells transplanted. PCR
confirmed
<1% (hALB detected in serum
of host animals)
0.0035-0.017% of host
hrpatocytes OCH1E5+ &/or
FISH+ for hDNA sequences.
Both human & mouse DNA
sequences not found in any of
5620 hepatocyted.
0.1-1.0%. hALB detected in
serum of transplanted mice.
“Low level” qualitatively. Both
human & mouse DNA not
found in individual cells..
Rare ALB+ cells qualitatively.
hALB detected in liver at
<0.01% in human liver.
Qualitatively, ~half of
periportal areas in half of
recipients. hALB in serum.
1.5-1.7% hALB+ /OCH1E5+ in
hosts receiving CD34+ hCB.
Less in others. 60-75% had
both human & mouse X
sequences.
0-0.8% hAFP+ or hALB+; 01.1% OCH1E5+
/detected but not quantified.
No cells expressed both mouse
and human ALB
12-58 hALB+/20 sections after
CCl4; 0-4 without CCl4. All
cells that expressed hALB also
expressed mouse CK18.
0 from CD34+ or CD34- hBM
nonMSC. MSC yielded
0.035+0.021% (max.), which
disappeared. Cells contained
mixtures of human & rat
chromosomes.
0-17% (time-dependent).
BM>CB>periph Bl. Sheep
blood contained hALB. None
of 150-500 cells had both
human & mouseDNA,.
Qualitatively detected, but
appear uncommon.
21.1+3.2% . Sheep serum
contained HuALB. Of 80
isolated OCH1E5+hepatocytes
all had only HuDNA,
suggesting lack of fusion.
2
S59.
hCB
S60
hCB
Preimmune
fetal goats/
None
Preimmune
fetal M/
None
0.5-5.0% BMC
expressed
human antigens
Not examined
OCH1E5+
Not quantified
Present but not quantified
OCH1E5+; hALB+; hAAT+
Not quantified
4-6% of livers contained
OCH1E5+ cells; fewer livers
had hALB+ cells