Product Specification
gRAD-RWB
For use with Rabbit antibody-derived detection conjugates
to analyse Whole Blood samples
Summary
In most applications, the specific test reagent
contains both a biotinylated capture reagent and a
detection conjugate. When the test reagent is
mixed with the sample, both the capture reagent
and detection conjugate react with the analyte
present in the sample to form a complex. The
sample mixture is applied to the round well of the
gRAD, blood cells or other particles are filtered
out and the liquid flows through the device,
passing the test line. The test line contains a
biotin-binding
protein
that
captures
both
complexed and free biotinylated capture reagent,
the ratio of the first to the second relating to the
amount of analyte present in the sample.
Sufficient capture reagent is present in the test
reagent to saturate the test line after running for
5-10 minutes. On saturation, the amount of
analyte complex immobilized at the test line
depends on its ratio to the free capture reagent,
not on the volume of sample that has crossed the
test line.
The round and square application wells
Apart from the round well, the gRAD has an additional square well which can be used when the detection
conjugate is inhibited by other components present in the sample (e.g. immune response assays in which the
detection conjugate binds all antibodies or a class of antibodies). In these cases the sample is mixed with the
biotinylated capture reagent before being applied to the square well, whereupon the detection conjugate is
added to the round well. The detection conjugate solution pushes the sample mixture in front of it through the
gRAD to the test line, where the biotin binding protein captures the biotinylated capture reagent. The rest of
the sample mixture containing the inhibiting components continues through the gRAD, leaving the capture
reagent-analyte complex at the test line ready to react with the detection conjugate that passes over it.
Control line - for rabbit antibody-derived detection conjugates
The gRAD-RWB is designed for use with rabbit antibody-derived detection conjugates in that the control line
contains antibodies that bind the mouse antibody which is responsible for immobilizing the detection conjugate
at the test line. A visibly coloured control line indicates that the sample mixture or sample mixture + detection
conjugate has passed correctly through the device.
Filter for whole blood or particulate samples
The gRAD-RWB is designed with a filter to permit the analysis of whole blood and samples containing
particulate matter. The gold colloid conjugate passes through the filter with the rest of the sample mixture to
interact with the test and control lines.
Supply
The gRAD-RWB is supplied in a plain white box of 40 units marked only with the batch number. All other
labelling is present on a plastic film wrapping that is easily removed, allowing you to put on your own labels as
required. Each unit is packed in a white foil pouch which is also marked only with a batch number to permit
your own labelling. The batch number is printed directly on the gRAD, as well as an broad arrow indicating the
direction in which the gRAD should be placed in reader. Sample identification can be written within the arrow.
Specifications
Dimensions:
Sample pad:
Sample type:
Test line:
Control Line:
Detection:
Application volume:
78 mm (L) x 20 mm (W) x 5mm (D)
Whole blood filter
Whole blood, serum, plasma, urine and other aqueous solutions.
Biotin specific
Rabbit IgG specific
Only for use with gold colloid-based detection conjugates
100-200 µl