Bhanupriya Madarampalli Ph.D.
1506 W. Covington Club Apt-4
Peoria IL 61614
Phone: (309) 634-8607
e-mail: bhanupriya.arun@yahoo.com
EDUCATION
2007
Ph.D., Virology (Plant Molecular Virology)
Sri Venkateswara University (SVU), Tirupati, INDIA
“Biological and molecular characterization of tospoviruses infecting
economically important crops in India and development of
transgenic tomato with nucleocapsid gene”.
2000
M.Sc., Virology
Sri Venkateswara University, Tirupati, India
1997
B.Sc., Botany, Zoology and Chemistry
Sri Venkateswara University, Tirupati, India
RESEARCH EXPERIENCE
June 2012 – Present
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Characterization of plant promoters driving insect resistance gene(s)/pathway in maize
and wheat
July 2007- June 2008
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Volunteer (Research)
Advisor: Dr. Eric Johnson
Crop Bioprotection Research
National Center for Agricultural Utilization
Research, ARS-USDA, Peoria IL
Post Doctoral Fellow (Government of India
sponsored fellowship)
Advisor(s): Drs. K.K. Sharma & P. Lava Kumar
Genetic Transformation Lab,
International Crop Research Institute for Semi-Arid
Tropics (ICRISAT),
Patancheru, Hyderabad, INDIA.
Evaluated transgenic events expressing viral coat protein gene using PCR, Reverse
transcription-PCR, Southern, northern and western blot analysis
Investigated the molecular mechanism underlying virus resistance in the transgenic plants
Outlined the studies to perform RNAi based viral plasmids/constructs
April 2002-October 2005
Research Fellow (Doctoral research)
Advisors: Dr. K. S. Ravi & Prof. P. Sreenvasulu
Molecular Virology Lab,
MAHYCO Research Center,
Jalna, Maharashtra, INDIA.
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Diagnosis and Characterized tospoviruses prevalent in India
Studied molecular variability of tospoviruses by cloning and sequencing viral coat
protein gene
Expressed viral recombinant protein in bacterial system and purified viral protein by biochemical techniques.
Developed robust sero-diagnostic tools for virus detection using antibodies raised against
recombinant viral coat protein
Constructed plasmids expressing viral genes using bioinformatics and molecular biology
techniques
Designed approaches to generate transgenic plants expressing viral coat protein gene
September 2001 – March 2002
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Research Fellow (Doctoral research)
Advisor: Dr. D.V.R. Reddy & Prof. P. Sreenvasulu
Mycotoxin Diagnostic and Virology Lab,
ICRISAT),Patancheru, Hyderabad, INDIA.
Diagnosis, isolation and characterization of peanut bud necrosis virus isolates
SPECIALIZATIONS / RESEARCH INTERESTS
 Recombinant DNA technology
 Plant Molecular Virology
 Plant Genetic Engineering
AWARDS AND HONORS

Gold medal receipt: University topper in M.Sc. Virology (2000) from SVU,
Tirupati, India
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Post Doctoral Fellowship (2007-2008) from Indian Institute of ScienceDepartment of Biotechnology, India
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Best poster award for presenting a poster titled: “Characterization of
Watermelon bud necrosis virus from Maharashtra" at "International Symposium
on Vector borne Viral Diseases", during February 7-10, 2006; ICRISAT,
Hyderabad, India.
RESEARCH SKILLS
 Molecular biology
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Nucleic acids isolation
Primer designing, PCR and revere transcription (RT)-PCR analysis
Cloning: Site-directed or PCR based ligation, bacterial transformation,
plasmid isolation and restriction digestion analysis
Sub-cloning of restriction digestion fragments into expression vectors
Nucleic acid and protein hybridization techniques: Southern, Northern,
Western and dot blot analysis
 Biochemical techniques
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Induction/expression of recombinant protein in bacterial system
Protein purification: Isolation of tagged (6xHIS-Tag) proteins from total
cell lysates using affinity chromatography methods (Nickel-NTA resin
based)
Denaturing and native PAGE
Western and dot blot analysis
ELISA: Direct, indirect, antigen coating and double antibody sandwich
 Bio-Informatics tools
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Vector NTI-6.0 software for sequence analysis
 Virology
 Conducting field surveys, identification and collection of plant viruses
 Diagnosis of plant viruses using serological (ELISA, Dot, Tissue imprint)
and molecular biology (PCR and hybridization based) techniques
 Characterization of plant viruses
 Maintenance of virus culture under green house conditions
 Virus transmission and experimental host range studies
 Purification of plant viruses
 Isolation of viral nucleic acid
 Cloning and sequencing of viral genome fragments
 Sequence comparison and analysis using bioinformatics tools
 Development of sero-diagnostic tools for detection of plant viruses.
 Evaluation of virus resistance transgenic plants under green house
conditions
 Plant Genetic Engineering
 Designing and construction of binary vectors/plasmids expressing plant
viral gene
 Agro-bacterium mediated transformation of the binary plasmid into plant
systems/genome
 Validating the stable integration of viral gene into the plant genome by
variety of molecular biology methods.
 Screening the transgenic plants under green house conditions
PUBLICATIONS AND CONFERECNE PRESENTATIONS
1. Suresh R Kunkalikar, Sudarsana Poojari, Bhanupriya M Arun, Prem A Rajagopalan,
Tsung-Chi Chen, Shyi-Dong Yeh, Rayapati A Naidu, Usha B Zehr, Kankanallu S
Ravi (2011). Importance and genetic diversity of vegetable-infecting tospoviruses
in India. Phytopathology, 2011 Mar: 101 (3): 367-376.
2. M. Bhanupriya, P. Sreenivasulu, Usha B Zehr and K. S. Ravi. "Biological and
molecular characterization of tospovirus isolates from India" presented at
"National Symposium on Molecular Diagnostic for the Management of Viral
Diseases", during October 14-16, 2004; Indian Agricultural Research Institute, New
Delhi, India.
3. M. Bhanupriya, P. Sreenivasulu, Usha B Zehr and K. S. Ravi "Natural occurrence
and genetic diversity of tospovirus isolates from India" at " VIII International
symposium on Thysanoptera and Tospoviruses", during September 11-15, 2005;
Asilomar Conference Grounds, Pacific Grove, California, USA.
4. M. Bhanupriya M, P. Sudarsana, P. Sreenivasulu, Usha B Zehr and K. S. Ravi.
"Characterization of Watermelon bud necrosis virus from Maharashtra" at
"International Symposium on Vector borne Viral Diseases", during February 7 - 10,
2006; ICRISAT, Hyderabad, India.
5. S.R. Kunkalikar, P Sudarsana, M. Bhanupriya, Prem A. Rajagopalan, S.D. Yeh,
R.A. Naidu, Usha B. Zher and K.S. Ravi
“Biological and molecular
characterization of vegetable infecting tospoviruses in India” at IXth International
Symposium on Thysanoptera and Tospoviruses, during 31st August – 4th September
2009, Sea World Resort, Queensland, Australia.
MEMBERSHIP AND CONFERENCES ATTENDED
2004 to 2006
2004
2006
2007
2007
Indian Virological Society, India
“National Symposium on Molecular Diagnostic for the
Management of Viral Diseases”, during 14-16 October 2004;
Indian Agricultural Research Institute, New Delhi, India.
"International Symposium on Vector borne Viral Diseases", during
7-10 February 2006; ICRISAT, Hyderabad, India.
“10th International Plant Virus Epidemiology Symposium
Controlling Epidemics of Emerging and Established Plant Virus
Diseases - The Way Forward” during 15-19 October 2007;
ICRISAT, Hyderabad, India
“RNAi in genome Control” during 12-14 December 2007; Center
for Cellular and Molecular Biology, Hyderabad, India.
REFERENCES
 Dr. K.K. Sharma
Principal Scientist
Genetic Transformation Lab,
ICRISAT, Patancheru – 503 324
Hyderabad
Andhra Pradesh, India
E-mail : k.sharma@cgiar.org
Phone: +91- 40-30713300
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Dr. K.S Ravi
Senior Principal Investigator - Molecular Virology,
Mahyco Research Center, Jalna-431203
Maharahstra, India.
E-mail: ravi.kankanallu@mahyco.com
Tel: +91-80-27700632 / 27737299
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Dr. P. Lava Kumar
Principal Scientist, Virology
IITA-Nigeria Ibadan,
PMB 5320, Ibadan Oyo State, Nigeria
Email: L.kumar@cgiar.org
Tel: +234 2 7517472
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Prof. P. Sreenivasulu
Department of Virology
School of Biological and Earth Sciences
Sri Venkateswara University, Tirupati
Andhra Pradesh, India
E-mail: pothursree@yahoo.com
Tel : +91-877-2249666, Ext.252