Principal Investigator/Program Director (Last, First, Middle):
Jiang, Jianfei
BIOGRAPHICAL SKETCH
Provide the following information for the key personnel and other significant contributors in the order listed on Form Page 2.
Follow this format for each person. DO NOT EXCEED FOUR PAGES.
NAME
POSITION TITLE
Jianfei Jiang, Ph.D.
eRA COMMONS USER NAME
Research Assistant Professor
JFJIANG
EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.)
INSTITUTION AND LOCATION
DEGREE
(if applicable)
YEAR(s)
B.S.
1991-1995
Biochemistry
Ph.D.
1995-2000
Pharmacology
2000-2003
Toxicology
2003-2005
Toxicology
Lanzhou University, Lanzhou, Gansu, China
Shanghai Institute of Materia Medica, Chinese
Academy of Sciences, Shanghai, China
University of Pittsburgh, Pittsburgh, PA
University of Pittsburgh, Pittsburgh, PA
Postdoctoral
fellow
Research
Associate
FIELD OF STUDY
Please refer to the application instructions in order to complete sections A, B, and C of the Biographical
Sketch.
A. Positions and Honors
1999 Outstanding Award of 4th Ph.D. Forum of New Medicine in China
2000 DIAO Scholarship of Chinese Academy of Science
2000 Excellent Award of 5th Meeting of Cancer Research Fund in China
2003 American Society of Toxicology
2003 American Society for Experimental Biology and Medicine
2005 American Radiation Research Society
2005 Research Assistant Professor, Environmental and Occupational Health, University of Pittsburgh, PA
B. Publications
1. Arroyo A, Modriansky M, Serinkan FB, Bello RI, Matsura T, Jiang J, Tyurin VA, Tyurina YY, Fadeel B,
Kagan VE. NADPH oxidase-dependent oxidation and externalization of phosphatidylserine during
apoptosis in DMSO-differentiated HL-60 cells: Role in phagocytic clearance. J Biol Chem. 277: 4996549975, 2002.
2. Jiang J, Claudette M. St Croix, Nancy Sussman, Qing Zhao, Bruce R. Pitt, and Valerian E. Kagan.
Contribution of Glutathione and Metallothionein to Protection Against Copper Toxicity and Redox Cycing:
Quantitative Analysis Using MT+/+ and MT-/- Mouse Lung Fibroblase Cells. Chem Res Toxicol 15:10801087, 2002.
3. Bayir H, Kochanek PM, Liu S-X, Arroyo A, Osipov A, Jiang J, Wisniewski S, Adelson PD, Graham SH,
Kagan VE. Increased S-nitrosothiols and S-nitrosoalbumin in cerebrospinal fluid after severe traumatic
brain injury in infants and children: Indirect association with intracranial pressure. J. Cerebral Blood Flow
and Metabolism. 23: 51-61, 2003.
4. Liu WJ, Jiang J, Xiao D, and Ding J. Down-regulation of Telomerase Activity via Protein Phosphatase 2A
Activation in Salvicine-Induced Human Leukemia HL-60 Cell Apoptosis. Biochemical Pharmacol. 64: 16771687, 2002
5. Kagan VE, Borisenko GG, Serinkan BF, Tyurina YY, Tyurin VA, Jiang J, Liu SX, Shvedova AA, Fabisiak
JP, Uthaisang W, Fadeel B. Appetizing rancidity of apoptotic cells for macrophages: oxidation,
externalization, and recognition of phosphatidylserine. Am J Physiol Lung Cell Mol Physiol. 285: L1-17,
2003.
6. Borisenko GG, Matsura T, Liu SX, Tyurin VA, Jiang J, Serinkan FB, Kagan VE. Macrophage recognition of
externalized phosphatidylserine and phagocytosis of apoptotic Jurkat cells--existence of a threshold. Arch
Biochem Biophys. 413: 41-52, 2003.
PHS 398/2590 (Rev. 09/04, Reissued 4/2006)
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Principal Investigator/Program Director (Last, First, Middle):
Jiang, Jianfei
7. Jiang J, Serinkan BF, Tyurina YY, Borisenko GG, Mi z, Robbins PD, and Kagan VE. Transfection of Jurkat
cells with pro-apoptotic peptide DP1 causes selective peroxidation and externalization of
phosphatidylserine associated with realease of cytochrome c from mitochondria. Free Radic Biol Med
35:814-825, 2003.
8. Liang C, Wu ZG, Ding J, Jiang J, Huang GZ, Du RZ, Ge JB. Losartan inhibited expression of matrix
metalloproteinases in rat atherosclerotic lesions and angiotensin II-stimulated macrophages. Acta
Pharmacol Sin 25:1426-1432, 2004.
9. Kagan VE, Borisenko GG, Tyurina YY, Tyurin VA, Jiang J, Potapovich AI, Kini V, Amoscato AA, Fujii Y.
Oxidative lipidomics of apoptosis: redox catalytic interactions of cytochrome c with cardiolipin and
phosphatidylserine. Free Radic Biol Med. 37:1963-1985, 2004.
10. Liu WJ, Zhang YW, Shen Y, Jiang J, Miao ZH, Ding J. Telomerase inhibition is a specific early event in
salvicine-treated human lung adenocarcinoma A549 cells. Biochem Biophys Res Commun 323: 660-667,
2004.
11. Jiang J, Borisenko GG, Osipov A, Martin I, Chen R, Shvedova AA, Sorokin A, Tyurina YY, Potapovich A,
Tyurin VA, Graham SH, Kagan VE. Arachidonic acid-induced carbon-centered radicals and phospholipid
peroxidation in cyclo-oxygenase-2-transfected PC12 cells. J Neurochem. 90:1036-1049, 2004.
12. Jiang J, Kini V, Belikova N, Serinkan BF, Borisenko GG, Tyurina YY, Tyurin VA, Kagan VE. Cytochrome c
Release is Required for Phosphatidylserine Peroxidation during Fas-Triggered Apoptosis in Lung Epithelial
A549 Cells. Lipids 39:1133-1142, 2004.
13. Gandley RE, Tyurin VA, Huang W, Arroyo A, Daftary A, Harger G, Jiang J, Pitt B, Taylor RN, Hubel CA,
Kagan VE. S-Nitrosoalbumin-Mediated Relaxation Is Enhanced by Ascorbate and Copper. Effects in
Pregnancy and Preeclampsia Plasma. Hypertension 45:21-27, 2005.
14. Wipf P, Xiao J, Jiang J, Belikova NA, Tyurin VA, Fink MP, Kagan VE. Mitochondrial targeting of selective
electron scavengers: synthesis and biological analysis of hemigramicidin-TEMPO conjugates. J Am Chem
Soc 127: 12460-12461, 2005.
15. Kagan VE, Tyurin VA, Jiang J, Tyurina YY, Ritov VB, Amoscato AA, Osipov AN, Belikova NA, Kapralov
AA, Kini V, Vlasova II, Zhao Q, Zou M, Di P, Svistunenko DA, Kurnikov IV, Borisenko GG. Cytochrome c
acts as a cardiolipin oxygenase required for release of proapoptotic factors. Nat Chem Biol. 1: 223-232,
2005.
16. Tyurina Y, Kini V, Tyurin V, Vlasova I, Jiang J, Kapralov A, Belikova N, Yalowich J, Kurnikov I, Kagan V.
Mechanisms of cardiolipin oxidation by cytochrome c: relevance to pro- and anti-apoptotic functions of
etoposide. Mol Pharmacol 70: 706-717, 2006.
17. Tyurina YY, Kapralov AA, Jiang J, Borisenko GG, Potapovich AI, Sorokin A, Kochanek PM, Graham SH,
Schor NF, Kagan VE. Oxidation and cytotoxicity of 6-OHDA are mediated by reactive intermediates of
COX-2 overexpressed in PC12 cells. Brain Res. 1093:71-82, 2006.
18. Bayir H, Fadeel B, Palladino MJ, Witasp E, Kurnikov IV, Tyurina YY, Tyurin VA, Amoscato AA, Jiang J,
Kochanek PM, DeKosky ST, Greenberger JS, Shvedova AA, Kagan VE. Apoptotic interactions of
cytochrome c: redox flirting with anionic phospholipids within and outside of mitochondria. Biochim Biophys
Acta. 1757: 648-659, 2006.
19. Kagan VE, Tyurina YY, Bayir H, Chue CT, Kapralov AA, Vlasova II, Belikova NA, Tyurin VA, Amoscato A,
Epperly M, Greenberger J, DeKosky S, Shvedova AA, Jiang J. The “pro-apoptotic genies” get out of
mitochondria: Oxidative lipidomics and redox activity of cytochrome c/cardiolipin complexes. Chem Biol
Interact. 163:15-28, 2006.
20. Jiang J, Kurnikov I, Belikova NA, Xiao J, Zhao Q, Amoscato AA, Braslau R, Studer A, Fink MP,
Greenberger JS, Wipf P, Kagan VE. Structural requirements for optimized delivery, inhibition of oxidative
stress, and antiapoptotic activity of targeted nitroxides. J Pharmacol Exp Ther 320:1050-1060, 2007.
21. Kagan VE, Jiang J, Bayir H, Stoyanovsky DA. Targeting nitroxides to mitochondria: location, location,
location and concentration. Free Radic Biol Med. 43: 348-350, 2007.
22. Belikova NA, Jiang J, Tyurina YY, Zhao Q, Epperly MW, Greenberger JS, Kagan VE. Cardiolipin-specific
peroxidase reactions of cytochrome c in mitochondria during irradiation induced apoptosis. Int J Radiat
Oncol. 69:176-186, 2007.
23. Fink MP, Macias CA, Xiao J, Tyurina YY, Jiang J, Belikova NA, Delude RL, Greenberger JS, Kagan VE,
Wipf P. Hemigramicidin-TEMPO Conjugates: Novel Mitochondria-targeted Anti-oxidants. Biochem
Pharmacol. 74: 801-809, 2007.
PHS 398/2590 (Rev. 09/04, Reissued 4/2006)
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Principal Investigator/Program Director (Last, First, Middle):
Jiang, Jianfei
C. Research Support
Active
NIH/NIAID
Validation of radioprotective effects of drugs identified by siRNA library screening CMCR
Role: Principal Investigator
NIH/NIAID
Mitochondrial Targeting Against Radiation Damage CMRC
Role: Collaborator
Human Frontier Science Program
Oxidative Lipidomics of Programmed Cell Clearance: from nematodes to humans
Role: Collaborator
Completed
NIH
Pulmonary Phosphatidylserine Oxidation in Apoptosis
Role: collaborator
PHS 398/2590 (Rev. 09/04, Reissued 4/2006)
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