EVALUATION OF THE DNA STABILITY AND GROW PARAMETERS OF BEAN
SEEDS OF ARMENIAN NPP LOCATION REGIONS.
N.K.Karapetyan, Y.B.Dalyan, K.K.Karamyan, P.O.Vardevanyan, E.S.Gevorgyan.
Yerevan State University, 375025, Yerevan, Armenia (e-mail: nelkh@ysu.am)
The goal of this work is the radiation monitoring of the beans selected from six areas
of Armenian NPP location regions was conducted. The bean is known as the accumulator of
radionuclides. The choice of regions was determined by direction of the wind rose in this
region.
We separated the analyzed territory on three zones: zone I- territory in the radius of
1-3 km from NPP, zone II – territory with radius of 3-5 km, zone III- located on 30 km radius
around NPP. For the control we used the bean from region 60 km away from NPP. The
specific content of the radionuclides was determined in this bean samples. We investigated
the grow physiology of the bean seeds (growth linear-weight parameters analysis). We
studied parameters of seeds quality, such as germination energy, germination, yield of empty
seeds; this let us to testify the viability of grains.
Our results have shown, that

zone I - the main growth parameters were decreased, and the yield of empty seeds was
increased, compared with control.

zone II – (at the leeward side of NPP) was detected a low suppression of the grow and
slight increase in the yield of empty seeds.

zone III - the beans sprouts were the same sizes and viability index, as the control.
DNA has been isolated from the dry sprouts. After that, we investigated DNA melting
characteristics. It is well known, that this method allows to establish defects of structure
(single and double stranded breaks, inter- and intramolecular crosslink’s) of irradiated DNA
molecules.
The results have shown that the parameters of DNA melting of beans from various
zones of NPP practically are not changed in comparison with control.
This work was supported by grant ISTC A-773.