CURICULUM VITAE Name: Work Address: Rania Anastasiou Agricultural University of Athens Laboratory of Dairy Science Iera Odos 75, 118 55 Athens Greece +30 210 527 4676 ranastasiou@aua.gr Tel.: e-mail: EDUCATION 1982 - 1987 1988 - 1993 MS in Biology. National and Kapodistrian University of Athens, Greece. PhD in Biochemistry. National and Kapodistrian University of Athens, Greece. SCOLARSHIPS 1988 - 1992 1990 - 1991 Institute of Biology. N.R.C. "Democritus" Scholarship for postgraduate studies. Volkswagen Foundation. Scientific research at the Institute of Botany, University of Hannover. RESEARCH EXPERIANCE 1985 – 1986 1990 - 1991 1988 - 1992 1993 – today Laboratory of Biology. National & Kapodistrian University of Athens, Greece Institute of Botany. University of Hannover, Germany Institute of Biology. N.R.C. "Democritus", Athens, Greece Laboratory of Dairy Research. Department of Food Science and Technology, Agricultural University of Athens, Greece SCIENTIFIC ACTIVITY A. PUBLICATIONS IN REFEREED JOURNALS 1. Anastassiou, R. and Agryroudi-Akoyunoglou J.H. (1995). Thylakoid-bound proteolytic activity vs exogenous azocoll substrate during chloroplast development in bean. Journal of Plant Physiology 145, 24-30. 2. Bei-Paraskevopoulou, T., Anastassiou, R. and Agryroudi-Akoyunoglou J.H. (1995). Circadian expression of the Light-Harvesting protein of Photosystem II in etiolated bean leaves following a single red light pulse: Coordination with the capacity of the plant to form Chlorophyll and the thylakoid-bound protease. Photosynthesis Research 44, 93-106. October 2010 1 3. Anastassiou, R. and Agryroudi-Akoyunoglou J.H. (1995). Thylakoid-bound proteolytic activity vs LHC-II apoprotein in bean. Photosynthesis Research 43, 241-250. 4. Tsakalidou, E., Anastasiou, R., Papadimitriou, C., Manolopoulou, E. and Kalantzopoulos G. (1998). Purification and characterization of an intracellular X-prolyl-dipeptidyl-aminopeptidase from Streptococcus thermophilus ACA-DC 4. Journal of Biotechnology 59, 203-211. 5. Tsakalidou, E., Anastasiou, R., Vandenberghe, I., Van Beeumen, J. and Kalantzopoulos G. (1999). Cell wall bound proteinase of Lactobacillus delbrueckii subsp. lactis ACA-DC 178. Characterization and specificity towards β-casein. Applied and Environmental Microbiology 65, 2035-2040. 6. Anastasiou, R., Papadelli, M., Georgalaki, M.D., Kalantzopoulos, G. and Tsakalidou E. (2002). Cloning and sequencing of the gene encoding X-prolyl-dipeptidyl aminopeptidase (PepX) from Streptococcus thermophilus strain ACA-DC 4. Journal of Applied Microbiology 93, 52-59. 7. Georgalaki, M., Papadelli, M., Anastasiou R., Kalantzopoulos, G. and Tsakalidou E. (2002). Purification, biochemical characterization and cloning of the intracellular X-prolyl-dipeptidyl aminopeptidase from Streptococcus macedonicus strain ACA-DC 191. Le Lait 82, 657-671. 8. Anastasiou, R., Leverrier, P., Krestas, I., Rouault, A., Kalantzopoulos, G., Boyaval, P., Tsakalidou E. and Jan, G. (2006). Changes in protein synthesis during thermal adaptation of Propionibacterium freudenreichii ssp. shermanii. International Journal of Food Microbiology 108, 301-314. 9. Anastasiou, R., Georgalaki, M., Manolopoulou, M., Kandarakis, I., De Vuyst, L. and Tsakalidou E. (2007). The performance of Streptococcus macedonicus ACA-DC 198 as starter culture in Kasseri cheese production. International Dairy Journal 17, 208-217. 10. Papadelli, M., Karsioti, A., Anastasiou, R., Georgalaki, M. and Tsakalidou E. (2007). Characterization of the gene cluster involved in the biosynthesis of macedocin, the lantibiotic produced by Streptococcus macedonicus. FEMS Microbiology Letters 272, 75-82. 11. Anastasiou R., A. Aktypis, M. Georgalaki, M. Papadelli, L. De Vuyst and E. Tsakalidou (2009). Inhibition of Clostridium tyrobutyricum by Streptococcus macedonicus ACA-DC 198 under conditions simulating Kasseri cheese production. International Dairy Journal 19, 330-335. 12. Georgalaki M., Manolopoulou, E., Anastasiou, R., Papadelli, M. and Tsakalidou E. (2009). Detection of Streptococcus macedonicus in Greek traditional cheeses. International Dairy Journal 19, 96-99. 13. Georgalaki M., Papadelli, M., Chassioti, E., Anastasiou, R., Aktypis, A., De Vuyst, L., Van Driessche, G., Devreese, B., Van Beeumen, J. and Tsakalidou E. (2010). Milk protein fragments induce the biosynthesis of macedocin, the lantibiotic produced by Streptococcus macedonicus ACADC 198. Applied and Environmental Microbiology, 76, 1143-1151. 14. Asteri I-A., Papadimitriou K., Boutou E., Anastasiou R., Pot B., Vorgias C.E. and Tsakalidou E. (2010). Characterization of pLAC1, a cryptic plasmid isolated from Lactobacillus acidipiscis and comparative analysis with its related plasmids. International Journal of Food Microbiology, submitted. 15. Asteri I-A., Boutou E., Anastasiou R., Pot B., Vorgias C.E., Tsakalidou E. and Papadimitriou K. (2010). Characterization of plasmid pPS1 isolated from Pediococcus pentosaceus: In silico evidence for the horizontal gene transfer of the σΒ-regulated in Gram-positive bacteria gsiB to lactic acid bacteria. Applied and Environmental Microbiology, submitted. October 2010 2 B. PARTICIPATION IN CONFERENCES INTERNATIONAL CONFERENCES July 1991, Aghia Pelagia, Crete NATO ASI Regulation of Chloroplast Biogenesis. Anastassiou, R. and Argyroudi-Akoyunoglou, J.H. Thylakoid-bound protease activity as monitored by azo-dye release from azocoll substrate is affected by thylakoid development and chlorophyll synthesis inhibitors. July 1991, Aghia Pelagia, Crete NATO ASI Regulation of Chloroplast Biogenesis. Agryroudi-Akoyunoglou, J.H., Bei, T., Triantaphyllopoulos, K. and Anastassiou, R. (1991). Regulation of photosynthetic unit assembly: Regulation of LH apoprotein transcription, translation and stabilization in thylakoids. October 1991, Tempe, Arizona ISPMB Satellite Conference on the Molecular Biology of Photosynthesis. Arizona State University. Argyroudi-Akoyunoglou, J.H and Anastassiou R. Thylakoid-bound peptidase of Phaseolus vulgaris. July 1992, Szeged, Hungary FESPP Workshop on the Environmental Factors Affecting Photosystem II Anastassiou, R. and Agryroudi-Akoyunoglou J.H. Thylakoid-bound protease activity responsible for degradation of LHC-II apoprotein. September 1992, Nagoya, Japan IX International Congress of Photosynthesis Agryroudi-Akoyunoglou, J.H., Anastassiou, R. and Bei-Paraskevopoulou T. Proceedings IX International Congress of Photosynthesis (Murata N., Ed.) III, 12.327, Kluwer Academic, Dordrecht. Heat-shock or red-light pulse induced circadian rhythm in the capacity for Chlorophyll synthesis in etiolated bean and pea leaves. Correlation with PCR and LHC-II apoprotein appearance-stabilization. September 1995, Kusadasi, Turkey NATO ASI Workshop. Current Advances in Genetics, Metabolism and Applications of Lactic Acid Bacteria Tsakalidou, E., Anastasiou, R., Oikonomou, P. and Kalantzopoulos G. Cell wall bound proteinase of Lactobacillus delbrueckii subsp. lactis ACA-DC 178. Characterization and specificity towards β-casein. June 2001, Ede, The Netherlands NIZO Dairy Conference On Food Microbes. From knowledge to application Georgalaki, M., Papadelli, M., Anastasiou, R., Κalantzopoulos, G. and Tsakalidou E. October 2010 3 Purification, biochemical characterization and cloning of the X-prolyl-dipeptidyl aminopeptidase from Streptococcus macedonicus strain ACA-DC 191. June 2001, Ede, The Netherlands NIZO Dairy Conference On Food Microbes. From knowledge to application Anastasiou, R., Papadelli, M., Georgalaki, M., Kalantzopoulos, G. and Tsakalidou E. Cloning and sequencing of the gene encoding X-prolyl-dipeptidyl aminopeptidase (PepX) from Streptococcus thermophilus strain ACA-DC 4. September 2002, Egmond aan Zee, The Netherlands FEMS 7th Symposium on Lactic Acid Bacteria. Genetics, Metabolism and Applications Anastasiou, R., Papadelli, M., Georgalaki, M., Kalantzopoulos, G. and Tsakalidou E. Cloning and sequencing of the gene encoding X-prolyl-dipeptidyl aminopeptidase(PepX) from Streptococcus thermophilus strain ACA-DC 4. March 2004, Prague, Czech Republic IDF Symposium on Cheese. Ripening, Characterization and Technology Anastasiou, R., Kousta, D., Manolopoulou, E., Georgalaki, M., Kandarakis, I. and Tsakalidou E. Steptococcus macedonicus ACA-DC 198 as adjunct starter in Kasseri cheese production. June 2004, Saint-Malo, France International Symposium on Propionibacteria and Bifidobacteria: Dairy and Probiotic Applications Anastasiou, R., Leverrier, P., Krestas, I., Rouault, A., Kalantzopoulos, G., Boyaval, P., Jan, G. and Tsakalidou E. Changes in protein synthesis during thermal adaptation of Propionibacterium freudenreichii ssp. shermanii strains isolated from traditional Graviera Kritis cheese. August 2005, Egmond aan Zee, The Netherlands 8th FEMS Symposium on Lactic Acid Bacteria. Genetics, Metabolism, and Applications Anastasiou, R., Georgalaki, M., Manolopoulou, M., De Malsche, W., Psirras, D., De Vuyst, L. and Tsakalidou E. The performance of Streptococcus macedonicus ACA-DC 198 as single and protective starter in Kasseri cheese production. August 2005, Egmond aan Zee, The Netherlands 8th FEMS Symposium on Lactic Acid Bacteria. Genetics, Metabolism, and Applications Papadelli, M., Georgalaki, M.D., Anastasiou, R., Karsioti, A. and Tsakalidou E. Sequencing of the macedocin biosynthesis regulon, a lantibiotic produced by Streptococcus macedonicus ACA-DC 198. June 2006, Nantes, France 1st International Symposium on Antimicrobial Peptides, Food, Veterinary and Medical Applications Georgalaki, M., Anastasiou, R., Papadelli, M., Devreese, B., Van Driessche, G., Van Beeumen, J. and Tsakalidou E. October 2010 4 Induction of Bacteriocin Production in Streptococcus macedonicus ACA-DC 198. June 2006, Saint Malo, France American Society for Microbiology. Conference on Streptococcal Genetics Papadelli M., Karsioti, A., Anastasiou, R., Georgalaki, M. and Tsakalidou E. Characterization of the gene cluster involved in the biosynthesis of macedocin, the lantibiotic produced by Streptococcus macedonicus. October 2007, Goslar, Germany The 11th International Conference on Culture Collections Manolopoulou E., Georgalaki, M., Anastasiou, R., Papadelli, M., Aktypis, A., Zoumpopoulou, G., Maragoudakis, P., Asteri, I., Papadimitriou, K., Kalantzopoulos, G. and Tsakalidou E. The ACA-DC Greek Collection of microorganisms. March 2008, Bern, Switzerland 5th IDF Symposium on Cheese Ripening De Vuyst L., Leroy, F., Anastasiou, R., Georgalaki, M. and Tsakalidou E. Streptococcus macedonicus, a multifunctional and promising species for cheese manufacture. September 2008, Aberdeen, Scotland 21st International ICFMH Symposium (Food Micro 2008) - Evolving Microbial Food Quality and Safety Papadelli M., Maragkoudakis P., Georgalaki M., Anastasiou R., Zoumpopoulou G. and Tsakalidou E. Safety evaluation of the macedocin producer Streptococcus macedonicus ACA-DC 198. GREEK CONFERENCES December 1992, Athens 35th Meeting of the Hellenic Society of Biochemistry and Molecular Biology. Anastassiou, R. and Agryroudi-Akoyunoglou J.H. Biochemistry and Biophysics Newsletter 35, 92-94. Thylakoid-bound protease activity: Detection, appearance during thylakoid biogenesis and characteristics. December 2001, Athens 53rd Meeting of the Hellenic Society of Biochemistry and Molecular Biology. Anastasiou, R., Papadelli, M., Georgalaki, M., Kalantzopoulos G. and Tsakalidou E. Cloning and sequencing of the gene encoding X-prolyl-dipeptidyl-aminopeptidase (PepX) from Streptococcus thermophilus ACA-DC 4. April 2005, Athens 1st Hellenic Congress of Food Biotechnology and Food Technology. Oral Presentation. Anastasiou, R., Georgalaki, M., Manolopoulou, E., De Malsche, W., Psirras, D., De Vuyst, L. and Tsakalidou E. Streptococcus macedonicus ACA-DC 198 as adjunct and protective starter in Kasseri cheese production. October 2010 5 March 2007, Athens 2nd Hellenic Congress of Biotechnology and Food Technology. Anastasiou, R., Papalexanderatou, Z., van Genderen, F., Wauters, G., Georgalaki, M., Papadelli, M., De Vuyst, L., and Tsakalidou E. Inhibition of Clostridium tyrobutyricum LMG 1285T by Streptococcus macedonicus ACA-DC 198. March 2007, Athens 2nd Hellenic Congress of Biotechnology and Food Technology. Oral Presentation. Georgalaki, M., Papadelli, M., Anastasiou, R., Vandecasteele, C., Amiradaki, E., Manolopoulou, E. and Tsakalidou E. Microbiota of the Greek traditional cheese ‘Arseniko’ from Naxos. Study of physiological properties related to technology. December 2007, Athens 59th Meeting of the Hellenic Society of Biochemistry and Molecular Biology. Anastasiou, R., Aktypis, A., Georgalaki, M., Papadelli, M. and Tsakalidou E. Inhibition of Clostridium tyrobutyricum by Streptococcus macedonicus ACA-DC 198. December 2007, Athens 59th Meeting of the Hellenic Society of Biochemistry and Molecular Biology. Georgalaki, M., Papadelli, M., Chassioti, E., Anastasiou, R., Devreese, B., Van Driessche, G., Van Beeumen, J. and Tsakalidou E. Induction of Bacteriocin Production in Streptococcus macedonicus ACA-DC 198. December 2007, Athens 59th Meeting of the Hellenic Society of Biochemistry and Molecular Biology Papadelli, M., Karsioti, A., Anastasiou, R., Georgalaki, M. and Tsakalidou E. The biosynthetic gene cluster of macedocin, the lantibiotic produced by Streptococcus macedonicus. October 2008, Athens 1st Greek Congress on Milk and Dairy Products Georgalaki, M., Manolopoulou, E., Anastasiou, R., Papadelli, M. and Tsakalidou E. Detection of Streptococcus macedonicus in Greek traditional cheeses. December 2008, Athens 1st National MicroBioKosmos (MBK) Conference Manolopoulou E., M. Georgalaki, R. Anastasiou, M. Papadelli, A. Aktypis, G. Zoumpopoulou, P. Maragoudakis, I. Asteri, K. Papadimitriou, G. Kalantzopoulos and E. Tsakalidou The ACA-DC Greek Collection of microorganisms. December 2008, Athens 1st National MicroBioKosmos (MBK) Conference Papadelli M., Maragkoudakis P., Georgalaki M., Anastasiou R., Zoumpopoulou G. and Tsakalidou Ε. October 2010 6 Safety Evaluation of the Macedocin Producer Steptococcus macedonicus ACA-DC 198. November 2009, Athens 60th Meeting of the Hellenic Society of Biochemistry and Molecular Biology Asteri I.-A., Papadimitriou K., Boutou E., Anastasiou R., Vorgias K. and E. Tsakalidou Sequencing and characterization of the novel cryptic plasmid pPS1 carrying a general stress protein isolated from Pediococcus pentosaceus. November 2009, Athens 60th Meeting of the Hellenic Society of Biochemistry and Molecular Biology Papadimitriou K., Anastasiou R., Boutou E., Vorgias K. and E. Tsakalidou Osmotic stress-resistant mutants of S. macedonicus preserve their ability to produce the bacteriocin peptide macedocin under hyperosmotic conditions. OTHER PUBLICATIONS Anastasiou, R., Georgalaki, M., Manolopoulou, E., De Malsche, W., Psirras, D., De Wuyst, L. and Tsakalidou E. (2005). The performance of Streptococcus macedonicus ACA-DC 198 as protective starter culture in Kasseri cheese production. Galaktokomia 16, 66-67. Georgalaki, M., Amiradaki, E., Tzirita, M., Anastasiou, R., Papadelli, M., Manolopoulou, E. and Tsakalidou E. (2007). Microflora of the Greek traditional cheese “Arseniko”. Galaktokomia 18, 68-71. LANGUAGES October 2010 English, French 7