ADCC and CDC Assay Services Quotation Form
Instructions
1. Please complete and email this form together with the supporting materials to bioassay@genscript.com
2. Our service representative will contact you with the quote
Customer Information
Name:
Phone:
Institution/Company:
Shipping address (needed to determine shipping cost):
Email:
ADCC Assay Service (SC1544)
Do You Need An Approved Monoclonal Antibody As Gold Standard?
Yes, I need.
And I will provide the antibody.
Yes, I need.
But I don’t have the antibody.
No, I don’t need.
Note: Rituximab (Rituxan) Trastuzumab (Herceptin), and Cetuximab (Erbitux) are available at GenScript.
Number of Test Antibodies:
Note: The gold standard would be also included, if you choose it.
Nature of Test Antibodies:
Human；
IgG1；
Mouse；
IgG2；
Human & Mouse Chimeric；
IgG3；
Fc fusion protein；
Others
Others
Name of Target Cell Lines:
Hot target cell line options:
(1) HER2+:
SK-BR-3;
BT-474;
MDA-MB-231;
(2) CD20+:
Daudi;
Raji
(3) EGFR+:
A431;
A549
(4) tmTNFα+:
JIMT-1;
SK-OV-3;
T-47D;
MCF-7;
MDA-MB-453;
immortalized tmTNFα+ stable cell line at GenScript
Others (please specify):
Note: There are over 250 tumor cell lines available at GenScript. Please contact us for detailed list.
Effector cell options:
Effector stable cell line (NK92 cells stably expressing FcRγ3A.158V/V)
Human peripheral blood lymphocytes (PBL)
Human blood NK cells purified from PBL
Other species
Mouse
Rat
Optimization of E/T Ratios:
a. Antibody concentration: please specify:
(μg/ml) (10μg/ml by default).
b. Number of E/T ratios :
Only one fixed E/T ratio.
Please specify:
.
PBL ADCC:
(50:1, 25:1, and 10:1 by default).
NK92/ FcRγ3A.158V/V or blood NK ADCC:
(10:1, 5:1, and 1:1 by default).
Dose-response Curve:
8 points (including 0) in triplicate.
Default starting concentration: 10 (μg/ml); dilution factor: 10 ;
Customized starting concentration:
(μg/ml); dilution factor:
Detection of Antigen Expression Level of Target Cell(s) on FACS (Optional):
Yes, I need.
No, I don’t need.
Please specify any special requirements:
Project Information
Is this project for grant application purpose?
When will the project start?
Immediately
Yes
No
Within one month
Within three months
Half a year later
Note: Final report includes protocol summary, plus concentration-response curve and EC50 value for each antibody.
CDC Assay Service (SC1545)
Do You Need An Approved Monoclonal Antibody As Gold Standard?
Yes, I need.
And I will provide the antibody.
Yes, I need.
But I don’t have the antibody.
No, I don’t need.
Note: Rituxan (Rituximab) is available at GenScript.
Number of Test Antibodies:
Note: The gold standard would be also included, if you choose it.
Nature of Test Antibodies:
Human；
IgG1；
Mouse；
IgG2；
Human & Mouse Chimeric；
IgG3；
Others
Fc fusion protein；
Others
Name of Target Cell Lines:
Hot target cell line options:
(1) CD20+:
(2) tmTNFα+:
Daudi;
Raji
immortalized tmTNFα+ stable cell line at GenScript
Others (please specify):
Note: There are over 250 tumor cell lines available at GenScript.
Please contact us for detail list.
Dose-response Curve:
8 points (including 0) in triplicate.
Default starting concentration: 10 (μg/ml); dilution factor: 10 ;
Customized starting concentration:
(μg/ml); dilution factor:
Please specify any special requirements:
Project Information
Is this project for grant application purpose?
When will the project start?
Immediately
Yes
Within one month
No
Within three months
Half a year later
Note: Final report includes protocol summary, plus concentration-response curve and EC50 value for each antibody.