10 x Vent buffer (Qiagen) i.e.100mM KCl100mM (NH4)2SO4200mM Tris-HCl pH 8.820mM MgSO41% Triton X-100
5mM dNTP
Hazardous Substances Policy - Assessment
CHEMICAL HAZARD AND RISK ASSESSMENT
School of Biosciences
Name of
supervisor
Dr S. Minchin
Assessor
Rita Godfrey
Assessment Number*
1397
Date of Assessment
11 / 6 / 01
Signature
Signature
Notes
A School COSHH form in Word is available on the School Server.
Available from the Health and Safety Unit.
Guidance on making an assessment is given in Making a Chemical Hazard and Risk Assessment.
Guidance is also available from Guidance on Completing the Chemical Hazard and Risk Assessment Form.
Use a continuation sheet to expand any section of this form in hard copy version.
1
LOCATION OF THE WORK ACTIVITY
2
PERSONS WHO MAY BE AT RISK
List names where possible
Labs G4 and G10
All occupants of the lab (Laura Tate, Jo Field, Dong Ling Zeng and Rita Godfrey)
3
ACTIVITY ASSESSED
4
MATERIALS INVOLVED
NAME
PCR Primer Extension.
AMOUNT
max/stored
Attach copies of data sheet(s)
HAZARD
RISK PHRASES
10 x Vent buffer (Qiagen)
i.e.
100mM KCl
100mM (NH4)2SO4
200mM Tris-HCl pH 8.8
20mM MgSO4
1% Triton X-100
1ml
NSH
5mM dNTP
2ml
NSH
Primer labelled with
[gamma 32P] ATP
20ul
RADIOACTIVE
Vent exo- DNA
Polymerase
0.5ml
No data
available but
probably NSH
Mineral oil
25ml
NSH
100% Ethanol
2.5 litres
HIGHLY
FLAMMABLE
R11 Highly flammable
80% Ethanol
50ml
HIGHLY
FLAMMABLE
R11 Highly flammable
3M Na Acetate pH5.2
5ml
NSH
No R no.s but avoid skin
contact and inhalation as
prolonged or repeated
exposure may cause
allergic reactions in
sensitive individuals.
HAZDAT NO***
BIOSCIENCESNO***
DNase Blue i.e
40% Formamide
5M Urea
5mM NaOH
1mM EDTA
0.025% Bromophenol
blue
0.025% Xylene cyanol
FF
5
5ml
TOXIC
IRRITANT
R61 May cause harm to
the unborn child
R40 Possible risk of
irreversible effects.
R36/37/38 Irritating to
eyes, respiratory system
and skin
261 (form
-amide)
INTENDED USE**
Give brief details and attach protocol/instructions
See attached sheet. This assessment does not cover the preparation and use of the gel , for that see assessment
number 1529
6
RISKS to HEALTH and SAFETY from INTENDED USE
From personal exposure or hazardous reactions. Refer to OELs, flash points, etc., as appropriate. Are pregnant women, breast-feeding
mothers especially at risk?
There is a risk of exposure to radiation [32P]
There is a fire risk from the ethanol.
There is a risk of contact with the DNase Blue
7
CONCLUSIONS ABOUT RISKS
Is level of risk acceptable? Can risk be prevented or reduced by change of substance/procedure? Are control measures necessary?
The radiation and fire risks need to be controlled. The amount of DNase Blue used is very small so the risk is very
small but some control is still required.
8
CONTROL MEASURES
Additional to Good Chemical Practice
Wear protective clothing and gloves throughout. Work behind a perspex screen in lab G4 (“hot lab”)
Monitor the work area before and after use. Carry tubes into lab G10 in a perspex box sufficient to contain any
radiation. Place the tubes in the PCR machine behind a perspex screen and place a perspex container over the
top of the tubes so that there is no risk of radiation exposure from the top of the tubes. Use the screen when
removing the samples at the end and transport them to the centrifuge in the pespex container. Work behind the
screen when putting the tubes in and taking them out of the centrifuge. Continue using the screen and perspex
container until the samples have been loaded onto the gel.
Wear gloves when handling DNase Blue and replace the tube cap as soon as possible.
Keep the ethanol away from all sources of ignition.
This work is covered by the University Radiation Scheme 1/P
9
INSTRUCTION/TRAINING
Specify course(s) and/or special arrangements.
University radiation safety course
10
MONITORING
Performance of control measures,
Check integrity of gloves. Check Geiger counter is working properly before use.
Personal exposure
Radiation film badge
Health Surveillance
11
WASTE DISPOSAL PROCEDURE
See School Server for Approved Procedure Document on specific Chemical Waste Disposal.
Radioactive waste is disposed of according to the University Radiation regulations. Single items up to 1uCi and
totalling less than 10uCi should be placed in the low level radioactive waste bin in lab G4.
Waste ethanol should be put in the non-halogenated waste solvent bottle.
12
REVIEW
Enter the date or circumstances for review of assessment (maximum review interval 5 years)
June 2006
13
EMERGENCY ACTION
TO CONTROL HAZARDS To stabilize situation eg spread absorbant on liquid spill; eliminate sources of ignition, etc.
Switch off all sources of ignition. Mop up with paper towels or spill kit if it is large.
If any radioactive components are spilled they should be mopped up with paper towels which are then bagged
and placed in the low level waste bin.
TO PROTECT PERSONNEL Evacuation, protection for personnel involved in clean-up, Special First Aid
Wear protective clothing, gloves and goggles when clearing up the spill.
The room should be ventilated until all vapours from ethanol have gone.
Clean-up/decontamination
TO RENDER SITE OF EMERGENCY SAFE
Radioactive spills should be decontaminated with Decon, diluted according to the instructions on the container.
When all radiation has been removed (monitor with a Geiger counter) the site can be washed with water.
CONTACT
Dr S. Minchin
PHONE
45438
10.10.00
*
**
***
Prefix T is used for Teaching Assessment Number.
Please include amount of chemicals used and how.
Hazdat No is the UNICOSHH datasheet report number.
Biosciences No is the Biosciences data sheet number.
UNICOSHH IS A CHEMICAL DATABASE ON THE HEALTH AND SAFETY UNIT SERVER.
BIOSCIENCES DATA SHEETS ARE AVAILABLE IN THE SCHOOL SAFETY OFFICE.