Lab Two
Bacterial Cell Structures
Bacterial Structures and Shapes
Simple Staining procedures
Part One - Bacterial Structures and Shapes
In this exercise, you will view prepared slides of bacteria that make capsules, spores/endospores, or
have unique shapes/structures beyond the standard rods or cocci you have seen so far. Review
lecture notes, Chapter 3 about all these microbes and structures.
Prepared Slides to View - Work In Pairs
Bacterial Capsules, 100X objective
Flagella
Fat/PHB bodies
Bacillus stained for endospores, 100X objective
Treponema or Spirochetes, unusual shape and structure, 100X objective
Clostridium
Staphylococcus
Anabaena (view at 40X) make 2 different kinds of cells - a smaller green one that is
photosynthetic and a larger colorless one that fixes nitrogen. Note and include BOTH cell types in
your final drawing.
Part Two - Slide preparation of Microbes
Procedures
Each person stains, Streptococcus, Serratia, and Yogurt - label slides as such
Mix 1 loopful of water with a loop of each bacteria and set slides aside to COMLETELY air-dry
Show your instructor your drying slides to confirm you have enough material on them
After you have finished ALL other exercises, pass dried slides through flame 3 times to heat-fix
Biology 318, Lab Two, 1
Biology 318 Worksheet Due Next Lab - Turn in Individually
Name:
(1) 12 pts. Prepared Slides: Bacterial Structures and Shapes.
100X Objective
Capsule
(a) Where located in cell:
(b) What is it made of?
(c) Function(s):
DRAW AN ARROW TO THE PART FEATURED!!!
Endospores
(a) Define this term:
(b) Function(s):
(c) Example microbe that uses:
DRAW AN ARROW TO THE PART FEATURED!!!
Treponema
(a) What is this shape:
(b) Name a disease caused by this organism:
Flagella
Identify the two types
Biology 318, Lab Two, 2
Fat/PHB bodies
(a) Function:
(b) Example microbe that uses:
DRAW AN ARROW TO THE PART FEATURED!!!
Clostridium
(a) What is this shape:
(b) Name a disease caused by this organism:
Staphylococcus
(a) What is this shape:
(b) Name a disease caused by this organism:
Anabaena
a) Draw and label the two cell types,
b) Identify the functions of each:
Biology 318, Lab Two, 3
(2) 6 pts. Stain Results
100X Objective
Serratia
Streptococcus
Yogurt
Biology 318, Lab Two, 4