Important Location Information Thursday, June 4th 7:30A Breakfast in the Lewis and Clark Room on the first floor of the Stoney Creek Inn 8:00 Registration, outside Lewis and Clark Room 8:45 Scientific presentations in the Lewis and Clark Room at the Stoney Creek Inn 12:00P Lunch will be in the Galley in the Stoney Creek Inn. This dining area is adjacent to the check-in desk as you enter the hotel. 5:30 Bus leaves Stoney Creek in for Dinner at Les Bourgeois Vineyards in Rocheport, Missouri. Transportation will be provided (15 miles). Dinner will be outside on the Gooseneck Deck at the Les Bourgeois Vineyards A-Frame. Friday, June 5th 7:00A Breakfast will be in the Lewis and Clark Room on the first floor of the Stoney Creek Inn 7:30 Registration, Outside the Lewis and Clark Room 8:00 Scientific presentations in the Lewis and Clark Room at the Stoney Creek Inn 12:00 A box lunch “to go” will be provided in the Lewis and Clark Room at the Stoney Creek Inn Directions to the Veterinary Medical Diagnostic Laboratory (a 3 mile drive) will be provided for the optional laboratory tour. Tours begin at 12:30 Les Bourgeois is located just one mile off of Interstate 70 in historic Rocheport, Missouri--just about 15 minutes from the University of Missouri Campus in Columbia. Take I-70 west to Exit 115. Take Highway BB towards Rocheport (right). The Les Bourgeois vineyard entrance is 1.1 miles from the highway on the left, before your reach Rocheport. Program, Thursday June 4 8:00-8:30: ON-SITE REGISTRATION, Lewis and Clark Room Presentations marked with an asterisk (*) are participating in the graduate student award competition. The presenter’s names are in italics. 8:45 WELCOME: Dean Neil Olson, Department Chair Dr. George Stewart, VMDL Director Dr. Alex Bermudez .................................................................................................................... 1 9:00 Prevalence of Coxiella burnetii and Listeria monocytogenes in Wisconsin bulk-tank milk-Suzanne Gibbons-Burgener, Francine Cigel, Ogi Okwumabua and Kathy Toohey Kurth, University of Wisconsin ............................................................................................ 7 9:20 Development of an InvaderPlus assay for the detection of pathogenic Leptospira and species specific Identification of Leptospira borgpetersenii serovar hardjo- Michael O’Connor and Ogi Okwumabua, University of Wisconsin……………………………………………….9 9:40 *BVD-Mucosal disease in a cow and calf – all in the family- Chantelle C. Bozynski, Susan K. Schommer and William J. Mitchell, University of Missouri…………………………………….…10 10:00 *Variation in viremic concentration among cattle persistently infected with Bovine Viral Diarrhea Virus- Jason S. Nickell, Bradley J. White, Robert L. Larson and David G. Renter, Kansas State University…………………………………………………………………………………..12 10:20 Break 10:40 *Transcriptional analysis and promoter characterization of host specifically expressed outer membrane protein genes of Ehrlichia chaffeensis- Lalitha Peddireddi, Chuanmin Cheng and Roman R. Ganta, Kansas State University……………………………………………………14 11:00 *Utilization of real time PCR for quantification of Ehrlichia canis rickettsemia during experimental canine monocytic ehrlichiosis- R.T. Stoffel, J.C. McClure, J.J. Schaefer, S.A. Ewing and R.W. Stich, University of Missouri and Oklahoma State University………………….16 11:20 *Comparison of three tetracycline antibiotic treatment regimens for carrier clearance of persistent Anaplasma marginale infection derived under field conditions- James B. Reinbold, Johann Coetzee and Roman Ganta, Kansas State University…………………………18 11: 40 *Efficacy of doxycycline clearance on Ehrlichia canis after treatment initiated during the three phases of experimental canine monocytic ehrlichiosis- J.C. McClure, M.L. Crothers, J. J. Schaefer, P.D. Stanley, Y. Rikihisa, G. R. Needham, S. A. Ewing and R. W. Stich, University of Missouri, Ohio State University, Oklahoma State University………....20 12:00 Lunch, the Galley 12:40 Science, policy, and politics of lead poisoning: a complex topic with complex implications - Guest speaker, John Schultz, 1Resource Scientist, Missouri Department of Conservation…………………………………………………………………………………………………………………………….22 1:20 *Trypanosoma theileri in bovine synovial fluid- Laura C. Cregar, Marlyn S. Whitney, Jeff W.Tyler, Munashe Chigerwe and Sarai Rivera-Garcia, University of Missouri……………….25 1:40 Case Study: Identification of a non-classical (bison) strain of Mycobacterium avium subsp paratuberculosis in Cervus elaphus subsp. nelsoni (Rocky Mountain Elk)- P. E. Dykema, S. Robbe-Austerman, B.V. Thomsen and R. Bidfell, NVSL, Ames IA and Oregon State University……………………………………………………………………………………………………………27 2:00 *The significance of Spa-type represented in swine Erysipelothrix rhusiopathiae bacterins for cross-species protection against erysipelas- Alaina L. Ingebritson, APHIS and University of Iowa……………………………………………………………………………………………….…28 2:20 *Detection of swine influenza virus in porcine oral fluid samples- Susan E. Detmer, Marie R. Gramer and Sagar M. Goyal, University of Minnesota……………………………………30 2:40 Determination of the Sample Type, Serologic Data and Culture Methods for the Detection of Canine Brucellosis- W.H. Fales, Gayle C. Johnson, Irene Ganjam, Audrey Rottinghaus and Thomas J. Reilly, University of Missouri……………………………………………..32 3:00 BREAK 3:20 *Comparison of Salmonella species isolation and antimicrobial resistance patterns from porcine tissues between 2003 and 2008- Kris A. Clothier and Joann M. Kinyon, Iowa State University……………………………………………………………………………………………………34 3:40 Purification and biochemical characterization of the recombinant Taylorella equigenitalis alkaline phosphatase- Thomas J. Reilly, Britain A. Abel, Tendai, Mutangadura, Michael J. Calcutt, Irene K. Ganjam and William H. Fales, University of Missouri……………………………………………………………………………………………………………………….36 4:00 Comparison of Listeria monocytogenes isolates from dairy milk and human cases in Wisconsin- Jacy Steele, Ogi Okwumabua, Tim Monson and Suzanne Gibbons-Burgener, University of Wisconsin………………………………………………………………………………………………..38 4:20 A Case of Suspected Caffeine Intoxication in Two Dogs Possibly Exposed to Waste from a Methamphetamine Laboratory- Tim J. Evans, Dae Y. Kim, George E. Rottinghaus and Wilson Rumbeiha, University of Missouri and Michigan State University……………………..40 5:30 Adjourn to Le Bourgeois Program, Friday June 5 7:30 ONSITE REGISTRATION, Columbia Room 8:00 Suspected Xylitol Toxicity in a Dog- Ingeborg M. Langohr, Vinicius S. Carreira, and Karen Brantman, Michigan State Diagnostic Lab and Michigan Veterinary Specialists..............42 8:20 A Case of Acute Organic Arsenic Intoxication in Cattle with Concurrent Exposure to Fluoride- Tim J. Evans, Dan Shaw, Gayle Johnson, George E. Rottinghaus, Wilson Rumbeiha and Paula Imerman, University of Missouri and Michigan State University………………….44 8:40 *Gastrointestinal Leiomyositis as a Cause of Pseudo-obstruction in Two Dogs- Chanran K. Ganta, Gordon A. Andrews, Kyathanahalli S. Janardhan, Kelli M. Almes, Emily Klocke, Shane Lyon and Brad M. DeBey, Kansas State University…………………………………………………………………....46 9:00 *Histoplasmosis in a Domestic Short-Haired Cat and Brief Review of Cases Reported in Nebraska from 1998 to Present- Elisa Salas, Alan Doster, Scott McVey, Julie Knoezer, David Steffen and Debra Royal, University of Nebraska……………………………..…………………48 9:20 *Case Study of Acinetobacter lwoffii isolated from a Huacaya alpaca- Rachel A. Wardlow, Brenda C. Love, Timothy A. Snider and Suzanne G. Genova, Oklahoma State University…………………………………………………………………………………………………………………..…50 9:40 A Non-invasive Technique for the Evaluation of the Effects of Xenoestrogens on Prepubertal Gilts- Tim J. Evans, David R. Ledoux, Trygve L. Veum, George E. Rottinghaus, Alex J. Bermudez and Mark R. Ellersieck, University of Missouri……………………..……………52 10:00 BREAK 10:20 Use of Digitized Photomicrographs in Quantifying Hepatic Lipidosis in Bovine Livers in the Presence and Absence of Ketosis- Gayle C. Johnson, William Chamberlin and John Middleton, University of Missouri……………………………………………………………………………..…54 10:40 Hepatosplenic T-cell leukemia in a cat- Linda M. Berent, William J. Mitchell, Charles E. Wiedmeyer and Kara Osterbur, University of Missouri………………………………………………...56 11:00 Non-Infectious Age-Associated Pathology of Wild-Type Rats: A Description of Two Interesting Cases- Angela B. Royal and Jennifer M. Hughes, University of Missouri…….58 11:20 Spontaneous Paratuberculosis in an Angora Rabbit- Dae Young Kim and William J. Mitchell, University of Missouri…………………………………………………………………………………...60 11:40 NCAAVLD business meeting 12:05 Presentation of Graduate Student Awards 12:30 Optional Tour, Veterinary Medical Diagnostic Laboratory, University of Missouri