Research Infrastructure and Environment Keck Medicine of USC is located on the University of Southern California Health Sciences Campus, a 30.8 acre site, consisting of the Keck School of Medicine of USC and the Keck Medical Center of USC. The Keck Medical Center of USC includes the Keck Hospital of USC and the USC Norris Cancer Hospital. The campus also includes the USC School of Pharmacy, the Center for Craniofacial Molecular Biology (CCMB) and the Occupational Therapy/Physical Therapy Division of the Herman Ostrow School of Dentistry and the Doheny Eye Institute. Directly adjacent is the brand new Los Angeles County-USC Medical Center, a 600 bed teaching hospital (replacement of old hospital). The medical school campus has several major research enterprises which are mentioned here so as to emphasize the rich intellectual environment. USC Norris Comprehensive Cancer Center Keck Hospital of USC Zilkha Neurogenetic Institute & Broad Center Figure 1. USC Health Sciences Campus The USC Norris Comprehensive Cancer Center is an NIH supported Comprehensive Cancer Center with a variety of Core facilities including transgenic animals, DNA sequencing, microarray/genomics and flow cytometry cores described below. Keck School of Medicine of USC hosts the centralized location of the Southern California Clinical Translational Science Institute (SC CTSI).This is a significant NIH supported institute focused on translational research and education. The Zilkha Neurogenetic Institute (ZNI) is an integral part of a broader USC neuroscience initiative promoting collaboration between researchers from diverse disciplines. Scientists at the Institute reach across boundaries to embrace methods and techniques from other fields of study, identifying new approaches to examine nervous system function so we may all better understand the underlying causes of neurological and psychiatric disorders. The Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research at USC focuses on embryonic stem cell biology, developmental mechanisms related to stem cell regulation, tissue regeneration and repair, tissue stem cells, and chemical genomics applied to stem cell research. One entire floor of this facility will be devoted to training, core laboratories, and the provision of space and facilities for workers from outside the Center to develop pilot projects and undertake collaborations with Center researchers. The Broad Center is now turning efforts to further development of regenerative medicine in four therapeutic focus areas of excellence in translational and clinical medicine at the Keck School. These areas of particular research strength at USC include ophthalmology, cardiovascular medicine, hematology and oncology, and liver/gastrointestinal diseases. USC has established the first dedicated USC Epigenome Center in the nation, occupying an entire floor of the new Harlyne Norris Research Tower of the USC/Norris Comprehensive Cancer Center. The Center is well-equipped and has outstanding bioinformatic capabilities and is a great resource for multidisciplinary research, education and training in genomics, epigenomics, bioinformatics and translational medicine. Peter Laird who pioneered the demonstration of a causal relationship between DNA methylation and mouse tumorigenesis, and Michel Stallcup who was the first scientist to demonstrate a causal link between histone modification and gene expression. Cellular Analysis Cores Bioreagents & Cell Culture Core Facility This core facility supports cancer related research that requires in vitro experiments, provides reagents for cell culture and prepares bioreagents that are produced by cells grown in the facility. The core grows suspension cultures of hybridoma cells in large quantities and produces purified monoclonal antibodies using a New Brunswick BioReactor, providing for substantial savings to the investigator. The core is in the process of developing and evaluating lipofectin preparations for cell transfection at highly reduced costs. Additional available services include the preparations of specialized serum products, LB medium, matrix coated culture dishes, and AmpicillinR plates. Studies will be conducted to evaluate the feasibility of producing recombinant proteins, cytokines and growth factors. This facility is housed within the Norris Comprehensive Cancer Center. Flow Cytometry and Immune Monitoring Core This core offers advice, technical support and equipment for performing flow cytometry and immune monitoring. The facility is housed within the Norris Comprehensive Cancer Center. The Non-Parenchymal Liver Cell Core This core provides freshly isolated or cultured Kupffer, hepatic and sinusoidal endothelial cells (KC: hepatic macrophages), hepatic stellate cells (HSC) and sinusoidal endothelial cells (SEC) from normal rats or rat models of liver injury. This facility is housed within the department of pathology and managed by the Southern California Research Center for ALPD & Cirrhosis. Molecular Analysis Cores CHLA Vector Core The CHLA Vector Core, re-established in January 2010, provides assistance in the design and construction viral vectors, as well as in the production of viral particles for gene-modified in vitro as well as in vivo animal studies. The Vector Core also provides consulting services to help researchers identify their gene transfer needs, determine the best way to produce gene-modified cells, and to assist in experimental troubleshooting. The CHLA Vector Core is located at the Children's Hospital Los Angeles campus at 4650 Sunset Blvd. DNA Core Facility This core provides DNA sequencing, oligonucleotide synthesis, protein sequencing and peptide synthesis services to researchers at USC and other institutions. The facility is housed within the Norris Comprehensive Cancer Center. Genomics Core Facility This core performs high throughput analyses of genetic (polymorphism/mutation) and epigenetic variations (DNA methylation) that underlie predisposition and progression to cancer. The facility is housed within the Norris Comprehensive Cancer Center. Proteomics Core Facility Established in 2008, this core facility provides services associated with the technologies of high-sensitivity and high-resolution mass spectrometry for protein and peptide analysis. The facility is housed in department of molecular microbiology & immunology space and is administered by the department of medicine. Transgenic/Knockout Mouse Core Facility This facility was established in 1993 to provide high-quality embryo and stem cell manipulation, microinjection and related services to the USC community, as well as outside researchers. The core handles the production of transgenics, gene targeting and the production of knockouts, construction of targeting vector design, preparation of DNA for injection and in vitro fertilization, re-derivation, as well as cryopreservation. USC Epigenome Center USC has established the first dedicated Epigenome Center in the nation, occupying an entire floor of the newly constructed Harlyne Norris Research Tower of the USC/Norris Comprehensive Cancer Center. This 8,500-square-foot facility encompasses a state-of-the-art laboratory (4,800 square feet) and expansive dedicated bioinformatics facilities, including office space for 30 staff programmers, computational biologists, statisticians, and bioinformatics faculty. The Epigenome Center currently has one operational Illumina Genome Analyzer, a second Genome Analyzer version 2 with paired-end read capability on order, and Univesity funds dedicated for the establishment of the Illumina BeadLab, which will be moved to the Center. Imaging Facilities Cell and Tissue Imaging Core Facility The primary function of this core facility is to provide assistance and training in the operation of a wide variety of instrumentation for cell and tissue imaging. The core facility also offers support for specimen preparation, thin sectioning techniques, embedding procedures, cryosectioning techniques, photography, digital photomicroscopy and photomicrography and computer aided graphics. This facility is housed within the Department of Ophthalmology and the Doheny Eye Institute and is co-administered by the Norris Comprehensive Cancer Center. Molecular Imaging Center The USC Molecular Imaging Center offers accessibility to a wide variety of state-of-the-art imaging instrumentation. The core’s mission is to increase awareness and accessibility for all scientists, as well as assist investigators with imaging research projects. This facility is administered by the department of radiology. Data Analysis & Management Bioinformatics Services The Norris Medical Library provides bioinformatics support services for USC investigators. Services range from consultation to workshops to support for software licenses, to email alerts. Division of Bioinformatics The Division of Bioinformatics was recently formed to address the increasingly critical role of genomics data in biomedical research. Division researchers are involved in development of new bioinformatics methodologies, as well as in collaborative research projects with other faculty inside and outside of USC. The Biostatistics Core Services This resource provides statistical support to clinical, basic science and cancer cause and prevention investigators. This support may range from simple advice, to participating in the design of the research project, to carrying out aspects of the data management and statistical analysis of a project. This resource is housed within the Norris Comprehensive Cancer Center. Statistical Consultation & Research Center The Statistical Consultation and Research Center (SCRC) is an Organized Research Unit at the University of Southern California which integrates statistical, epidemiological and computing resources and offers them to professionals conducting clinical, biomedical and translational research. The SCRC consists of a team of statisticians, epidemiologists, database developers, programmers, project coordinators and data managers that provides an optimal structure to assist both the public and private sectors in carrying out clinical and prevention trials, observational and retrospective studies, crosssectional and longitudinal surveys and translational research. Members of the SCRC are co- investigators on a range of studies in atherosclerosis, cancer, diabetes, neuroscience, ophthalmology, stroke rehabilitation interventions, lifestyle redesign interventions and alternative medicine. Human Studies Support Clinical Investigations Support Office This office provides centralized administration for protocol development, review, prioritization, regulatory compliance, study conduct and monitoring, and quality assurance. It is comprised of a centralized Protocol Administration Unit and a Research Nursing/Data Management Unit. The resource is housed within the Norris Comprehensive Cancer Center. Clinical Trials Unit The Los Angeles Basin Clinical Translational Science Institute (LAB CTSI) Clinical Trials Unit (CTU) provides research services and support for a variety of clinical investigations – from diabetes to seizure disorders.