Work Instruction
WI0011
Preparation of Amine-Functionalized Glass Slides
Rev: D
1. Materials
1.1. Name: WI0003, Preparation of Piranha-Washed Glass Slides
1.2. Name: No. 1 cover glass, 43mm X 50 mm
1.2.1. Manufacturer: Electron Microscopy Sciences
1.2.2. Catalog: 3329
1.3. Name: 3-Aminopropyltriethoxysilane (APTES)
1.3.1. Manufacturer: Sigma
1.3.2. Catalog: A-3648
1.4. Name: Toluene
1.4.1. Manufacturer: Sigma-Aldrich
1.4.2. Catalog: 244511
1.5. Name: Absolute Ethanol
1.5.1. Manufacturer: Aaper
2. Procedure
2.1. Piranha wash the glass slide (WI0003).
2.2. Make a solution 5% by volume APTES in toluene. Pour enough to fully
immerse slide.
2.3. Immerse slide and incubate for 1 hr at room temperature.
2.4. Rinse under 100% ethanol to remove all residual toluene and APTES.
2.5. Rinse under DI water.
2.6. Blow dry.
2.7. Cure on a hot plate at the highest setting for 1 hr. If the hot plate temperature can
be set, set to 160 ºC. Use a piece of glass beneath so that the slide does not come
in contact with the dirty hotplate.
2.8. The slide can be stored at this point. Clean immediately before use to rinse off
residual, unlinked APTES.
2.9. Rise with 100% ethanol.
2.10.
Rinse with DI water.
2.11.
Blow dry.
3. References
3.1. Howarter, J.A. and J.P. Youngblood, Optimization of silica silanization by 3aminopropyltriethoxysilane. Langmuir, 2006. 22(26): p. 11142-7.
4. Revision History
Date
Rev Description
Revision Author
7/23/07 Initial release
Brian J. Schmidt
7/24/07 A
Adjust APTES concentration from 10% to Brian J. Schmidt
5%, yielded better results. Removed toluene
rinse step, not necessary at this
concentration.
7/25/07 B
Added curing and rinse steps.
Brian J. Schmidt
9/01/07 C
Updated format.
Brian J. Schmidt
2/24/08 D
Added references section
Brian J. Schmidt
bme.virginia.edu/lawrence
Date: 2/24/08
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