PI Name:__________________
Date:____________
9.2 Plasmid vectors (transferable genetic elements, capable of autonomous replication within a suitable host):
QUESTION
Name of plasmid
construct?
Vector 1
Vector 2
Vector 3
Vector 4
Name of inserted nucleic
acid?
Source of nucleic acid
inserted?
Function of inserted
nucleic acid?
Discuss risks associated
with this inserted nucleic
acid if it were
accidentally transferred to
an unintended host
(e.g. oncogenic,
immunogenic, toxic,
immune suppressive,
angiogenic, an allergen,
etc)
List any key expression
control elements (human
promoter, enhancer, etc.)
List ALL host(s) the
plasmid will be
transferred into-list
genus/species where
applicable (e.g. cell
lines, prokaryotes ,
plants, animals, insects,
etc):
How will it be transferred
to each host?
(e.g. transformation,
conjugation,
electroporation, injection,
particle bombardment,
etc)
How much will be
transferred to each host
(concentration and
volume)?
9.2 Recombinant/Synthetic Nucleic Acid ver. 09/13
PI Name:__________________
How did you obtain the
plasmid vector:
Date:____________
a.
Bought a commercial
kit Yes
No
a.
Bought a commercial
kit Yes
No
a.
Bought a commercial
kit Yes
No
a.
Bought a commercial
kit Yes
No
Which:
Which:
Which:
Which:
b.
Made all components
in my lab
Yes
No
b.
Made all components
in my lab
Yes
No
b.
Made all components
in my lab
Yes
No
b.
Made all components
in my lab
Yes
No
c.
Assembled in my lab
from components
made/obtained
elsewhere
Yes
No
c.
Assembled in my lab
from components
made/obtained
elsewhere
Yes
No
c.
Assembled in my lab
from components
made/obtained
elsewhere
Yes
No
c.
Assembled in my lab
from components
made/obtained
elsewhere
Yes
No
Obtained from:
Obtained from:
Obtained from:
Obtained from:
d.
d.
d.
d.
Received cells
already containing
the plasmid
Yes
No
Obtained from:
Received cells
already containing
the plasmid
Yes
No
Obtained from:
Received cells
already containing
the plasmid
Yes
No
Obtained from:
Received cells
already containing
the plasmid
Yes
No
Obtained from:
Attach a map of all vector constructs at the end of the document
9.2 Recombinant/Synthetic Nucleic Acid ver. 09/13