Plant FTIR Results
Plant FTIR
1.10
a
1.00
b
0.90
c
0.80
absorbance
f
d
g
e
h
0.70
i
j
0.60
k
l
0.50
m
0.40
0.30
3900.00
3400.00
PRP-8 LEAF
2900.00
2400.00
PRP-8 VEIN
1900.00
PRP-8 ROOT
1400.00
PRP-11 GREEN
900.00
PRP-12 BROWN
0.20
400.00
Table 1 Interpretation of Plant FTIR profile
Peak
Wavenumber
Description
broad, strong
intensity
Possible functional groups
O-H Stretching(3350±150);NH2
Stretching(3300±100)
CH3 Stretching(2962±10),CH2
Stretching(2926±10), C-H Stretching(2961)
-COOH Overtone (2500±300),-COOH
Stretching (3000±500)
a
3370-3570
b
2900-2960
sharp peak
c
2850-2870
sharp peak
d
2352&2364
e
1727
f
1608-1670
medium
intensity, sharp
with saw tooth
g
1465
medium
intensity, sharp
with saw tooth
CH2 bend(1465)
h
1383
medium
intensity, sharp
CH3 bend (1375±10)
i
1180
medium
intensity
j
1000-1100
weak
k
904
l
671
m
435
medium
intensity, sharp
with two peaks
medium
intensity, sharp
with saw tooth
weak
medium
intensity, sharp
medium
intensity with saw
tooth
CO2
C=O (1727) aldehyde
H2O bending(1650±30);C=C Stetching
(1640±20,1670±10); Aromatic Ring Stretching
(1950±10);
Comments
Since it's broad and strong intensity, this is
due to O-H bond rather than NH2 bond
This part is generally accept as acid dimer
or group as -COOH in cellulose study
Source
Wang et al
2006
Wang et al
2006
the dip between two peaks could be well
explained by the CO2
For PRP-8 root, this peak is more obvious
which could be explained by less cellulose
oxidation noise
Saw tooth are highly possible products of
cellulose (H-C-OH) partial oxidation (16171745). Meanwhile, same peak in the leaf
sample (PRP-8) are smoother. Water should
also be considered as a possible results
Possible part of cellulose, also more evident
for stem and root samples instead of PRP-8
leaf sample
This peak is most obvious in the leaf
sample. The saw tooth in the rest samples
could possible be both xyloglucan(1371) and
cellulose(1362)
J.Løjewska et
al 2005
M.Kačuráková
et al 2002
M.Kačuráková
et al 2002
Most likely CH3 bending.
C-O Stretching, CCstretching(1115,1100,1075,1060,1042,1030,10
15)
=CH2 bending(909)
Aromatic bending(690±20)
S-S Stretch (430-500)
Literature ascribes small peaks in this range
as cellulose, pectin, xyloglucan
This peak could only be explained as a
standard cellulose peak according to
literatures (894), also it is most obvious on the
brown sample
Most likely C6, very sharp peak is found at
Leaf Sample
Most possible Aryl disulfides, sharp peak is
found at the green sample
M.Kačuráková
et al 2002
Zhong et al
2000
Wang et al
2006
Coates 2000
Soil FTIR Results
Soil FTIR - Norm alized
1
0.9
b
c
n
0.7
l
h
d
e
f
0.6
m
0.5
g
j
0.4
0.3
k
0.2
0.1
0
4000
3500
15
3000
2500
16
2000
Wavelength (cm-1)
17
1500
1000
18
0
500
7
Absorbance
a
0.8
i
Table 2 Interpretation of Soil FTIR profile
Peak
Wavenumber
Description
Possible functional groups
O-H stretching region (3800-3400 for clay
mineral),
O-H stretching region (3800-3400 for clay
mineral)
a
3700
sharp peak
b
3622
sharp peak
c
3464
broad, strong
intensity
O-H , N-H
c
2935
tiny broad
C-H (3150-2850)
e
2372 & 2347
f
g
2011
1901
tiny saw tooth
peaks
weak
weak
h
1655
medium intensity
i
1450 & 1400
weak
j
1099-1034
k
800
l
696
m
540
n
472
sharp &
strongest peak
medium intensity,
saw tooth
medium intensity,
sharp
medium intensity,
sharp
strong intensity,
sharp
Comments
Source
Stuart
Bands due to Si-O-O-OH vibration.
Since it's broad and strong intensity, this is
due to O-H bond rather than N-H bond.
The peak is below 3000, so it is an aliphatic CH vibration. Medium intensity absorptions at
1450 and 1375 cm-1 will indicate -CH3 bend
stretching.
CO2 bend
CO2 bend
C=C (1680-1600 for aromatic and
alkenes); C=O vibrations (1680-1630 for
amide), C=N (1690-1630) and also of N-H
bend (1650-1475); water absorbance (~
1640)
Some soil literature assigned this to C=O
vibrations of carboxylates and aromatic.
Vibrations involving most polar bonds, such as
C=O and O-H have the most intense IR
absorptions. This peak has medium intensity and
most likely due to water.
C-H, alkenes, -CH3 (bend, 1450 and
1375), -CH2 (bend, at 1465),
Most likely CH3 bending.
Si-O vibration of clay minerals
Consistent with FTIR spectra of soil in the
literature
NH2 wagging and twisting, =C-H bend,
alkenes
N-C=O bend for secondary amides
C-C=O bend for secondary amides, SiO3-2
Intense absorption at 460-475 corresponds to
SiO3-2 vibration. In the literature, bands at
800,780,650,590,530 and 470 are attributed to
inorganic materials, such as clay and quartz
minerals.
Gerzabek et al
2006, Lojewska et
al. 2005
Gerzabeck et al.
2006
Stuart
Grasshopper Soil FTIR Results
Grasshopper FTIR
0.65
a
0.60
e
b
0.55
f
absorbance
c
0.50
g
h
d
0.45
i j
k
0.40
l
0.35
m
0.30
0.25
3900.00
3400.00
2900.00
2400.00
1900.00
wavenumber
1400.00
900.00
0.20
400.00
Table 3 Interpretation of Grasshopper FTIR profile
Peak
Wavenumber
a
3430
b
2967
c
2933
d
2346&2360
Description
broad, strong
intensity
medium
intensity
medium
intensity
medium
intensity with two
peaks
sharp
Possible functional groups
O-H Stretching(3350±150);NH2
Stretching(3300±100)
CH3 Stretching(2962±10)
CH2 Stretching(2926±10)
CO2
H2O bending(1650±30);N-H bending(15901650), =N-H bending,(1550-1660),C=C
Stretching(1640±20),β sheet
e
1650
f
1556
g
1471
h
1407
i
1251
j
1182
weak
CH3 bending
k
1097
weak
C-O Stretching; C-C Stretching(1150±25,
1125±25)
l
682
m
418
medium
intensity with saw
tooth
medium
intensity with saw
tooth
medium
intensity
medium
intensity with saw
tooth
medium
intensity, sharp
medium
intensity with saw
tooth
Aromatic nitro compounds (1485-1555);
Aliphatic nitro compounds(1540-1560);
Carboxylate acid salt(1550-1610)
Comments
Since it's broad and strong intensity, this is
due to O-H bond rather than NH2 bond
Hydrocarbons have major absorption bands at
2800–3000
Hydrocarbons have major absorption bands at
2800–3000
Source
Gibbs 2002
Gibbs 2003
the dip between two peaks could be well
explained by the CO2
Possible the influence of water , if water
influence is not considered, this peak should
be Amide I (1600-1700), literature points out
1655 should be Amide I which is mainly
composition of Chitin
Literature shows no peaks around 15001600 is ascribed to Chitin
Iconomidou et
al 2001
Iconomidou et
al 2002; Coates
2000
Methylene C-H bending(1445-1485);Aromatic
ring stretching(1450-1510)
Ammonium ion(1390-1430)
not a peak of Amine or Amide
C-N stretching(1250-1340);P=O
stretching(1250-1350)
this peak should be Amide III (1230-1320),
also mainly composition of Chitin
Coates 2000
Same peak is also found in plant samples,
so here we would not ascribe it into Amine and
Amide group
Coates 2000
Aromatic bending(690±20)
Most likely C6,same peak found in plant
samples
Coates 2000
S-S Stretching (430-500)
Most possible Aryl disulfides, same peak
found in plant samples
Coates 2000