SYLLABUS FOR MOLECULAR BIOLOGY
GLEN PALMER
JOY STURTEVANT
gpalme@lsuhsc.edu
jsturt@lsuhsc.edu
OF EUKARYOTIC PATHOGENS MICRO 231
DS7339B
MEB6207
941-8426
568-6116
Major objectives:
 Importance of eukaryotic pathogens
 Challenges of molecular eukaryotic pathogen research
 Techniques and approaches used
o How to choose a technique/approach
o Design of approach, appropriate controls, pitfalls
o Interpretation
 Exposure to major areas of molecular eukaryotic pathogen research
Part I
Emphasis is on how current methods are utilized to identify or functionally analyze virulence
attributes of eukaryotic pathogens.
 Explanation of method: history, rationale, how/why it works
 Brief summaries of specific examples of investigations conducted with fungal or parasitic
pathogens where possible.
 Journal articles will be assigned for some lectures
Part II and III:
Each section will include a ‘participatory’ lecture. Most sections will also include a journal club
led by a student.
Additional journal articles may be assigned for reading prior to class.
Part II
Fungal pathogenesis
Part III
Parasite pathogenesis
EVALUATION
40 % - class participation
20 % - midterm – journal article critique (more info later)
40 % - final – a ‘wee’ problem (more info later)
For midterm and final – you will not have to know any of the details we present (in the absence
of notes/articles). However, you will need to understand the lectures so you can use this
information to understand the journal articles and the final ‘problem’ you will be assigned.
CLASSES: MON/ WED 9 – 10:30, LARGE MIP CONFERENCE ROOM
PART1.
METHODS IN EUKARYOTIC PATHOGENESIS AND APPLICATIONS
WED AUG 13
INTRO TO EUKARYOTIC PATHOGENS.
MON AUG 18
WED AUG 20
CONSIDERATIONS FOR MOLECULAR INVESTIGATIONS I
CONSIDERATIONS FOR MOLECULAR INVESTIGATIONS II
PALMER
PALMER
MON AUG 25
WED AUG 27
GENE DELETION AND GENETIC MANIPULATION I
GENE DELETION AND GENETIC MANIPULATION II
PALMER
KELLY
MON SEP 1
LABOR DAY
WED SEP 3
EPITOPE TAGS AND REPORTER GENES
WANG
MON SEP 8
WED SEP 10
GENOMICS I
GENOMICS II
STURTEVANT
KELLY
MON SEP 15
GENE LIBRARIES AND IVET
STURTEVANT
WED SEP 17
TRANSCRIPTOMICS I
FOX
MON SEP 22
WED SEP 24
PROTEOMICS AND PROTEIN:PROTEIN INTERACTIONS
PROTEOMICS AND PROTEIN:PROTEIN INTERACTIONS
MON SEP 29
WED OCT 1
MIDTERM EXAM – JOURNAL CRITIQUE AND PRESENTATION*
MIDTERM EXAM – JOURNAL CRITIQUE AND PRESENTATION*
PART 2
FUNGAL PATHOGENESIS
MON OCT 6
WED OCT 8
ANTIFUNGAL DRUG RESISTANCE I
ANTIFUNGAL DRUG RESISTANCE II
FOX
FOX
MON OCT 13
WED OCT 15
IMPORTANCE OF FUNGAL MORPHOGENESIS I
IMPORTANCE OF FUNGAL MORPHOGENESIS I
PALMER
PALMER
MON OCT 20
WED OCT 22
CRYPTOCOCCAL VIRULENCE I
CRYPTOCOCCAL VIRULENCE I
WANG
WANG
MON OCT 27
WED OCT 29
THE SEARCH FOR A VACCINE (CELL WALL) I
THE SEARCH FOR A VACCINE (CELL WALL) II
CUTLER
CUTLER
STURTEVANT/KELLY
I
I
FOX
FOX
(STAFF)
(STAFF)
PART 3
PARASITE PATHOGENESIS
MON NOV 3
WED NOV 5
PARASITE ENTRY, IMMUNE EVASION I
PARASITE ENTRY, IMMUNE EVASION II
STURTEVANT
STURTEVANT
MON NOV 10
WED NOV 12
KINETOPLASTID VIRULENCE FACTORS I
KINETOPLASTID VIRULENCE FACTORS I
KELLY
KELLY
MON NOV 17
WED NOV 19
MOLECULAR EVENTS IN PARASITES, IMPLICATIONS IN VIRULENCE I
MOLECULAR EVENTS IN PARASITES, IMPLICATIONS IN VIRULENCE II
KELLY
KELLY
MON NOV 23
WED NOV 26
PLASMODIUM GENETICS
COURSE SUMMATION
AIYAR
(STAFF)
MON DEC 1
WED DEC 3
MON DEC 8
WED DEC 10
FINAL: STUDENT PRESENTATION*
FINAL: STUDENT PRESENTATION*
FINAL: STUDENT PRESENTATION*
FINAL: STUDENT PRESENTATION*
* CLASS MAY LAST UNTIL 11:00 AM INSTEAD OF 10:30
(STAFF)
(STAFF)
(STAFF)
(STAFF)