Standard Operating Procedure for preparation and
processing of water collection equipments
Supervised by: Dr.John Capece
Southern DataStream Company
Labelle - Florida – U.S.A
Complied by: Hasna Benchiguer
May 2006
Table of Content
1. Introduction ..................................................................................................................... 3
2. Washing jars and Caps .................................................................................................... 3
2.1 Steps for cleaning jars ............................................................................................... 3
2.2 Steps for cleaning tops .............................................................................................. 7
3. Filter holder cleaning ...................................................................................................... 9
4. ISCO sample bottles cleaning ....................................................................................... 10
1
List of figures
Figure1. Dishwasher at park house………………………………………………………3
Figure2. Jars in the dishwasher…………………………………………………………..4
Figure3. Jars covered with rack………………………………………………………….4
Figure4. Flipped jars……………………………………………………………………..5
Figure5. Dishwasher soap and their emplacement………………………………………5
Figure6. Dishwasher turn on steps………………………………………………………6
Figure7. Dishwasher panel………………………………………………………………6
Figure8. Lab grade soap (Micro 90)…………………………………………………….7
Figure9. Liner and cap (top)…………………………………………………………….7
Figure10. Paint remover (solvent)……………………………………………………...8
Figure11. Filter holder with o-ring……………………………………………………...9
2
1. Introduction
This SOP is a protocol to be followed by working trainee of Southern DataStream Inc.,
for preparation and processing of sampler equipments.
2. Washing jars and Caps
Jars are cleaned in the dishwasher installed at park house laboratory immediately after
finishing samples processing.
Figure1. Dishwasher at park house.
2.1 Steps for cleaning jars
1. Separate and empty jars.
2. Load immediately dishwasher with jars, load the bottom of dishwasher first.
(67 jars approximately).
3
Figure2. Jars in the dishwasher.
3. Cover jars with rack to avoid flipped jars and their water filling as showed in
figure 4.
Figure3. Jars covered with rack.
4
Figure4. Flipped jars.
● Flipped jars.
● Water filling jars.
4. Put soap powder in both emplacement of this:
No enclosed
emplacement
Enclosed
emplacement
Figure5. Dishwasher soap and their emplacement.
a.) Wash 1 time (normal, hot, no dry) cycle with automatic soap.
b.) Wash 1 time with half cap special soap (lab soap micro 90) in the enclosed
emplacement of soap.
c.) Wash 1 time without soap, water only.
5
5.
1
2
3
4
Figure6. Dishwasher turn on steps
1
2
3
4
Figure7. Dishwasher panel.
6
Figure8. Lab soap (Micro 90)
5. Rinse jars with deionized water (DI water).
2.2 Steps for cleaning tops
1. Split cap and liner.
Figure9. Liner and cap (top)
7
2. Remove the label with solvent.
Figure10. Paint remover (solvent)
3. Wash caps and liner in clothes washing machine:
a.) 1 time with clothes soap
b.) 1 time with water
c.) Rinse with DI water
4. Reassemble caps and liner
PS: wash jars, liners, caps simultaneous
5. Reassemble (close) jars with tops while wet
6. Store in clean plastic bag to avoid contamination
8
3. Filter holder cleaning
You need 4 buckets arranged in the following sequence:
-
First bucket should contain tap water with lab grade soap
-
Second bucket should contain clean tap water
-
Third bucket should have DI water
-
Last bucket should also have DI water
1. Open filter holder by rotating anti-clockwise
2. Remove dirty/used filter papers
3. Wash both part of filter holder with brush in the first bucket.
4. Rinse the filter holder in clean tap water (2nd bucket).
5. Then rinse in the first DI water (3rd bucket).
6. Rinse finally in the second DI water (4th bucket).
7. Check to ensure there is O-ring in at least one part of the filter holder.
Figure11. Filter holder with o-ring
8. Place filter paper in the bottom part of the filter holder.
9. Add some drop of DI water on the filter to avoid the filter membrane slipping.
10. Attach the top part of the filter to the bottom.
9
4. ISCO sample bottles cleaning
1. Clean with tap water and lab grade soap (Micro 90) using a brush if necessary, to
remove particulate matter or surface film.
2. Rinse thoroughly with tap water.
3. Rinse thoroughly with DI water. Enough water shall be used to ensure that all
equipments surfaces are flushed with water.
4. Allow to air dry as long as possible.
5. Cap and store in clean bag.
Clean sampling bottles, equipments, shall be wrapped in plastic bags to prevent
contamination during storage or transport to the field.
If no further sampling is to be performed, equipment must be put in plastic bags.
10