How Do Plants Grow: Gaining, Transforming and Losing Plant Mass 1. What are the materials that contributed to the radish seedlings mass? How did the plant get those things? Look at this young tree planted in a bucket of soil. As the tree grows it gains weight. Think about whether the soil is food for the plant. 2. Do you think the weight of this tree came mostly from materials the plant took from the soil? YES NO 3. Predict whether the weight of the soil in the pot will “increase”, “decrease”, or stay the “same” as the plant grows: In the following paragraph, van Helmont describes his experiment. “I took an earthen pot and in it placed 200 pounds of earth which had been dried out in an oven. This I moistened with rain water, and in it planted a shoot of willow which weighed five pounds. When five years had passed the tree which grew from it weighed 169 pounds and about three ounces. The earthen pot was wetted whenever it was necessary with rain or distilled water only. It was very large, and was sunk in the ground, and had a tin plated iron lid with many holes punched in it, which covered the edge of the pot to keep air-borne dust from mixing with the earth. I did not keep track of the weight of the leaves which fell in each of the four autumns. Finally, I dried out the earth in the pot once more, and found the same 200 pounds, less about 2 ounces. Thus, 164 pounds of wood, bark, and roots had arisen from water alone." (Howe 1965) 4. Copy the table and write down the changes in weight of the tree and the soil. WEIGHT CHANGE OF TREE WEIGHT CHANGE OF SOIL 5. How would you explain the results that von Helmont found? Where does the majority of a plant’s mass come from if not the soil? 6. Why did the soil lose some mass? What components of the soil might now be somewhere else? 7. Although von Helmont was able to show that plants didn’t simply take mass from the soil for all of their growth, he believed that instead the plant’s material was somehow composed of water, the only thing that he had added to the bucket other than soil. Why is that idea incomplete? 8. What process describing plant growth was unknown to him and other scientists of the time that we now take for granted? 9. What is the main product of this process that contributes to plant mass? 10. As you consider any plant, though, it is obvious that although it has both water and this product, it is more complex than either of those things. Can you think of any other molecules that make up a plant’s dry material, and where within the plant or its cells those molecules might be found? 11. What was van Helmont's reason for conducting the experiment? In other words, what question did he set out to answer? 12. What was van Helmont's hypothesis? 13. Briefly restate in your own words the experiment performed by van Helmont. 14. List at least five variables that might affect this experiment. Which variable(s) is(are) controlled? Which is the experimental variable(s)? 15. Identify the experimental group and control group in this experiment. 16. Seedlings use energy to grow structures like stems and roots before plants develop leaves. What do you think is the source of this energy? 17. The +light, + water seedlings were constantly under light. Do you think these plants were carrying out cellular respiration? Explain your answer. 18. To summarize all that we’ve discussed, please copy and complete the following table: Gaining Mass Transforming Mass Losing Mass Which process is responsible? What is the effect on CO2 around the plant? What is the main product(s) of the process? 19. Read page 107 in your textbook. How do Joseph Priestly’s, Saussure’s and von Mayer’s results modify van Helmon’t conclusions?