Virtual Cell Learning Objectives Draft (ETC Excerpt)
Ganesh Padmanabhan
3/25/2004
Module ETC:
1. The learner should recognize that the ETC is a process that is auxiliary to ATP
production.
2. The learner should recognize that the ETC’s role in ATP production is to pump
hydrogen ions out of the matrix space and into the inter-membrane space.
3. Given a cross-sectional diagram of the inner mitochondrial membrane and a
visual representation of the concentration of hydrogen ions on both sides of the
membrane, the learner should be able to identify which way if any would the ions
flow if the membrane were removed.
4. The learner should be able to identify which terms amongst those involved in
photosynthesis and other cellular processes are directly involved in ATP
synthesis.
5. The learner should be able to diagram a mitochondrion and label the outer
membrane, inner membrane, inter-membrane space, and matrix.
6. Given a diagram of a mitochondrion, and ATP Synthase oriented properly across
the inner membrane, the learner should be able to orient a hydrogen ion pump
appropriately so that ATP Synthase can produce ATP.
7. The learner should recognize that the following terms are related to ATP
Synthesis and the ETC: O2, H2O, ATP, NADH, NADH Dehydrogenase,
Ubiquinone, Cytochrome b-c1, Cytochrome c, Cytochrome oxidase, ATP
Synthase
8. Given a list of the electron carriers in the ETC (NADH, NADH dehydrogenase,
ubiquinone, cytochrome b-c1, cytochrome c, cytochrome oxidase, H2O/O2), the
learner should be able to identify which ones pump ions across the membrane.
9. Given a list of the electron carriers in the ETC (NADH, NADH dehydrogenase,
ubiquinone, cytochrome b-c1, cytochrome c, cytochrome oxidase, H2O/O2), the
learner should be able to identify which ones shuttle electrons between proton
pumps in the membrane.
10. Given a list of the electron carriers in the ETC (NADH, NADH dehydrogenase,
ubiquinone, cytochrome b-c1, cytochrome c, cytochrome oxidase, H2O/O2), the
learner should be able to identify which one is the final recipient of electrons.
11. Given a list of the electron carriers in the ETC (NADH, NADH dehydrogenase,
ubiquinone, cytochrome b-c1, cytochrome c, cytochrome oxidase, H2O/O2), the
learner should be able to identify which ones are not bound by the membrane..
12. Given a list of the electron carriers in the ETC (NADH, NADH dehydrogenase,
ubiquinone, cytochrome b-c1, cytochrome c, cytochrome oxidase, H2O/O2), the
learner should be able to identify which ones bring electrons out of the ETC.
13. Given a list of the electron carriers in the ETC (NADH, NADH dehydrogenase,
ubiquinone, cytochrome b-c1, cytochrome c, cytochrome oxidase, H2O/O2), the
learner should be able to identify which ones bring electrons into the ETC.
14. Given a diagram with ETC components labeled, the learner should be able to
identify where ascorbate, FADH, and NADH can donate electrons.
15. The learner should be able to calculate how many protons would be pumped if an
electron enters the ETC from NADH, ascorbate, or FADH.
16. Given a variant of an ETC where one of the three proton pump complexes
(NADH dehydrogenase, cytochrome b-c1, cytochrome oxidase) pumps a
different, but given number of protons, the learner should be able to calculate how
many protons would be pumped if an electron enters the ETC from NADH,
ascorbate, or FADH.
17. Given a damaged ETC system and information on that system’s performance
when particular substrates are added, the learner should be able to identify
whether the data provided is sufficient evidence to conclude whether a specified
component is dysfunctional.
18. Given a damaged ETC system and insufficient evidence to conclude which
component is dysfunctional, the learner should be able to describe an experiment
using only the substrates provided and procedures to replace components with
functioning analogs that would generate the evidence needed.
19. The learner should be able to identify whether electrons “enter” the ETC from the
matrix space or inter-membrane space.
20. The learner should be able to identify whether electrons “exit” the ETC on the
matrix space or the inter-membrane space.
21. For each proton pump component in the ETC, the learner should be able to
identify both from which carrier it receives electrons and to which carrier it
donates electrons.
22. For each electron carrier shuttle, the learner should be able to identify between
which two proton pumps the carrier shuttles electrons.