PSI Community Outreach Proposal
Applicant:
Target is nominated by [insert name] of [institution]
We request that this project is carried out by the Northeast Structural Genomics
Consortium
Target:
[protein name] [# amino acids] [MW]
[insert amino acid sequence]
Structural homologues:
Sequence alignment of [target] and [homologue] the protein with most similar sequence
already in the PDB [PDB ID], shows < [% ID] sequence identity over [# residues] residues:
[insert sequence alignment]
Rationale:
[insert biological relevance / community value]
NMR or X-ray Crystallographic Protocols:
[known experimental parameters and suggested protocols]
Protein Purity, Stability, Concentration:
[evidence of protein purity, stability, foldness (e.g.: MS, NMR, and CD data)]
Expression:
[protocols and resources]