Supplementary Table 3: Comparison of size variation, efficacy and induction response of synthetic and native
promoters
Serial
Number
Name of
Promoter
1
4×W2
Size of
promot
er
(bp)
112
Sources of promoter
Size of
Native
Promoter
2
4×W1
120
PR1 Gene Parsley
840 bp
3
4 ×D
124
PR2 Gene Parsley
-------
PR1 Gene Parsley
840 bp
Induction
Condition
Reference
(Rushton et
al. 1996)
4
4×S
96
ELI7.1 Gene Parsley
ELI7.2 Gene Parsley
5
4 × GST
100 bp
GST 1 Potato
6
EE/ FF
CaMV35S
7
FFEE
CaMV35S
8
C18
49 bp
HVA1 Gene
68 bp
pathogenderived
peptide
elicitor,
pep25
pathogenderived
peptide
elicitor,
pep25
pathogenderived
peptide
elicitor,
pep25
pathogenderived
peptide
elicitor,
pep25
pathogenderived
peptide
elicitor,
pep25
Rhizoctonia
solani
Sclerotina
sclerotioru
m
20μM ABA
9
C19
49 bp
HVA1 Gene
68 bp
20μM ABA
10
C20
49 bp
HVA1 Gene
68 bp
20μM ABA
11
C21
49 bp
HVA1 Gene
68 bp
20μM ABA
12
C22
51 bp
HVA1 Gene
68 bp
20μM ABA
380 bp
1400bp
(Rushton et
al. 1996)
(Rushton et
al. 1996)
(Kirsch et al.
2000)
(Strittmatter
et al. 1996)
(Kirsch et al.
2001)
(Heise et al.
2002)
(Hong et al.
1988)
(Hong et al.
1988)
(Hong et al.
1988)
(Hong et al.
1988)
(Hong et al.
13
GT1NOS101
165 bp
pea rbcS-3A promoter
and Nopaline synthase
gene
410 bp
14
Z-NOS101
147 bp
1120 bp
15
G-NOS101
153 bp
Z-motif of the
Arabidopsis cab1
promoter and
Nopaline synthase
gene
Arabidopsis cab
promoter and
Nopaline synthase
gene
16
GATANOS101
149 bp
1721 bp
17
G/GATANOS101
197 bp
Arabidopsis rbcS
promoter and
Nopaline synthase
gene
-----
18
GT1/GATANOS101
209 bp
------
------
19
4H1-46
21 bp
Histone gene promoter 681 bp
20
mPtDrl02
1041 bp
PtDrl02 promoter
derived from poplar
[(Populus tomentosa
x P. bolleana)
x P. tomentosa]
986 bp
21
Triple-Op
117 bp
one operator (O1)- 1
bp up- stream of the
TATA-box, a second
operator (O2)- 1 bp
downstream of the
350 bp
950 bp
-----
white light
(100
mmol/m2/s)
and blue
light
white light
(100
mmol/m2/s)
1988)
(Mitra and
An 1989)
(Mitra and
An 1989)
white light
(100
mmol/m2/s)
and blue
light
white light
(100
mmol/m2/s)
(Mitra and
An 1989)
Far red
light, red
light and
blue light
Far red
light, red
light and
blue light
(NaCl, 0.25
M), ABA
(100 pm )
2,4-D (2.40, 100 pm)
and 6-BAP
(100 pm)
Methyl
jasmonate
(200 μM)
(Mitra and
An 1989)
Tetracycline
(0.1 mg/l)
(Gatz et al.
1991)
(Mitra and
An 1989)
(Mitra and
An 1989)
(Mikami et
al. 1987)
(Zheng et al.
2010)
22
pCAR46C
(4 x 38
bp =152
bp)
23
6 x XRE-P
109 bp
24
4xNRE-minGUS
172 bp
25
DR5(8×)
88 bp
TATA-box and a third
operator (O3)- 23 bp
downstream of the
TATA-box fused to
CaMV
35 S promoter
Chalcone synthase
(chs15) promoter
277 bp
six tandem repeated
XRE sequences of
CYP1A1
gene
Nitrite reductase
(NIR1) promoter
3.1 kb
AuxRE from the GH3
promoter
592 bp
3.1 kb
p-coumaric
acid
(5 x 10-4
M)
3-methylCholanthren
e (5 nM)
(Ryder et al.
1987)
10 mM
KNO3 and
10 mM
NH4NO3
αnaphthalene
acetic
acid (50
μM)
(Konishi and
Yanagisawa
2010)
(Xu and
Pasco 1998)
(Liu et al.
1994)
References:
Gatz C, Kaiser A, Wendenburg R (1991) Regulation of a modified CaMV 35S promoter by the
Tn10-encoded Tet repressor in transgenic tobacco. Molecular & general genetics : MGG 227:
229-237
Heise A, Lippok B, Kirsch C, Hahlbrock K (2002) Two immediate-early pathogen-responsive
members of the AtCMPG gene family in Arabidopsis thaliana and the W-box-containing elicitorresponse element of AtCMPG1. Proceedings of the National Academy of Sciences of the United
States of America 99: 9049-9054
Hong B, Uknes SJ, Ho TH (1988) Cloning and characterization of a cDNA encoding a mRNA
rapidly-induced by ABA in barley aleurone layers. Plant molecular biology 11: 495-506
Kirsch C, Takamiya-Wik M, Schmelzer E, Hahlbrock K, Somssich IE (2000) A novel regulatory
element involved in rapid activation of parsley ELI7 gene family members by fungal elicitor or
pathogen infection. Molecular plant pathology 1: 243-251
Kirsch C, Logemann E, Lippok B, Schmelzer E, Hahlbrock K (2001) A highly specific
pathogen-esponsive promoter element from the immediate-early activated CMPG1 gene in
Petroselinum crispum. The Plant journal : for cell and molecular biology 26: 217-227
Konishi M, Yanagisawa S (2010) Identification of a nitrate-responsive cis-element in the
Arabidopsis NIR1 promoter defines the presence of multiple cis-regulatory elements for nitrogen
response. The Plant journal : for cell and molecular biology 63: 269-282
Liu ZB, Ulmasov T, Shi X, Hagen G, Guilfoyle TJ (1994). Soybean GH3 promoter contains
multiple auxin-inducible elements. Plant Cell 6, 645-657.
Mikami K, Tabata T, Kawata T, Nakayama T, Iwabuchi M (1987) Nuclear protein(s) binding to
the conserved DNA hexameric sequence postulated to regulate transcription of wheat histone
genes. FEBS letters 223: 273-278
Mitra A, An G (1989) Three distinct regulatory elements comprise the upstream promoter region
of the nopaline synthase gene. Molecular & general genetics : MGG 215: 294-299
Rushton PJ, Torres JT, Parniske M, Wernert P, Hahlbrock K, Somssich IE (1996) Interaction of
elicitor-induced DNA-binding proteins with elicitor response elements in the promoters of
parsley PR1 genes. The EMBO journal 15: 5690-5700
Ryder TB, Hedrick SA, Bell JN, Liang XW, Clouse SD, Lamb CJ (1987) Organization and
differential activation of a gene family encoding the plant defense enzyme chalcone synthase in
Phaseolus vulgaris. Molecular & general genetics : MGG 210: 219-233
Strittmatter G, Gheysen G, Gianinazzi-Pearson V, Hahn K, Niebel A, Rohde W, Tacke E (1996)
Infections with various types of organisms stimulate transcription from a short promoter
fragment of the potato gst1 gene. Molecular plant-microbe interactions: MPMI 9: 68-73
Xu C, Pasco DS (1998) Suppression of CYP1A1 transcription by H2O2 is mediated by
xenobiotic-response element. Archives of biochemistry and biophysics 356: 142-150
Zheng H, Lin S, Zhang Q, Lei Y, Hou L, Zhang Z (2010) Functional identification and
regulation of the PtDrl02 gene promoter from triploid white poplar. Plant Cell Rep 29: 449–460.