The University of Jordan
Faculty of Agriculture
Biotechnology in Plant Protection
606950
Dept. of Plant Protection Instructor: Staff members.
Coordinator: Prof. H. Khlaif.
Course description
The purpose of this course is to give the students an idea about the most recent technique in biotechnology and molecular biology employed in the different fields in plant protection.
Week
1
I : Introduction:
Subject
2-5 1DNA, RNA, and protein structures.
2DNA replication and recombination.
3Profiling techniques for identification of plant pathogens. isolation of DNA, PCR, RFLP, and AFLP.
4Protein profiles, electrophoresis, serology and fatty acids composition …… etc.
5Molecular genetics of pathogenicity of phytopathogenic bacteria enzymes extrapolysaccharides, plant growth substances and toxins.
6Plasmids and their role in phytopathogenic bacteria.
6-7 II :Biotechnology in plant nematology.
1Molecular diagnosis of plant parasitic nematodes.
2Phylogenetic analysis using molecular data.
3Molecular biology of host pathogen interaction (recognition, penetration, colonization, virulence, and resistance mechanisms)
8-9 III :1-Methods for the recovery and quantitative estimation of propagules from soil.
2-Quantification of vesicular arbuscular mycorrhizae in plant roots.
3-Production of endomycorrhizal inoculum
4-Procedure for inoculation of plants with VMA in laboratories, green house, and field.
5-Evaluation of plant response to colonization by VMA fungi.
6-Spore germination and axenic culture of endomycorrhizae.

10-13 VI :1-Recent advances in agrochemicals.
2-Mass rearing of natural enemies
3-Pheromones: Identification, Extraction, and application.
4-Insect identification using molecular techniques.
14-15 V: 1- Herbicides resistance in plants.
2-Allelochemicals in plant protection.
Course outcome:
At the end of the course the students should be able to:
1Be familiar with the structure of DNA, RNA, and protein.
2Replication and recombination of DNA.
3Most recent topics in biotechnology and molecular biology.
4Be familiar with PCR, RFLP, AFLP, Serology, Elisa,....
etc., in detection, identification and separation of plant pests into strains.
5Virulence factor, of plant pests.
6Molecular basis of resistsance for the different pests.
7Molecular basis for host-pathogen interaction.
Grading System:
1rst hr Exam
2nd hr Exam
30%
30%
Final Exam 40%
References:
1.
Hampton, R., E. Ball, S. De Boer. 1993. Serological methods for detection and Identification of Viral and Bacterial Plant
Pathogens.APS Press.
2.
Madigan, T.M., Martinko, M.J. and Jack Parker. 2003: Brock/
Biology of Microorganisms. Tenth edition, Southern Illinois
University, Carbon dale. pp 1019.
3.
Newton, C.R. and A. Graham. 1995. PCR. Bios Scientific
Publisher.pp 161.
4.
Nickle, W-R. 1991. Manual of Agricultural Nematology. Dekker. pp.1035.

5.
Voss, G. and G.Ramos, ed. 2003. Chemistry of Crop Protection.
Wiley-VCH Verlag Gmblt & Co Germany.
6.
Watson, J.D., Gilman, M., Witkowski, J. and Zoller, M. 1992.
Recombinant DNA. W.K. Freeman and company, New York.pp
626.
7.
Sambrook, J., Russell, W.D. 2001. Molecular cloning. Laboratory
Manual, 3 rd edition 1,2,3. Cold Spring Harbor. Laboratory Press.
8.
11 th IUPAC International Congress of Pesticide Chemistry, Kobe,
Japan.2006.