7930 Arjons Drive, Suite B
San Diego, CA 92126
Phone: 858-6788683
Fax:
800-3804198
Email: orders@gentarget.com
Lentiviral service request form
(Please Fax / Email the completed form to the address above)
Customer contact Information:
You Name:
Institute / Company:
Address:
Phone:
Email:
Service Description:
Gentarget provide service to generate: expression lentivirus,
shRNA
knockdown lentivirus, promoter specific signaling lentivirus; anti-microRNA
lentivirus, or microRNA expression lentivirus. Lentivirus titer is >= 1 x 107
IFU/ml.
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For over-expression, we will sub-clone the customer selected genes into
Gentarget’s expression lentivectors, and sequentially produce 0.5 ml expression
lentivirus.
For shRNA knockdown expression, we will design and clone shRNA target
sequences into our shRNA lentivectors, and sequentially produce 0.5 ml of each
shRNA lentivirus.
For anti-microRNA, we will construct the anti-miRNA lentivector that
expresses the anti-sense to the selected miRNA, and sequentially produce 0.5
ml shRNA lentivirus.
For signaling lentivirus, we will clone your desired promoter sequence into
our lentivector to drive a fluorescent marker or luciferase marker, and
sequentially produce 0.5 ml shRNA lentivirus.
For microRNA expression, we will sub-clone the selected precursor microRNA
along with its native flanking sequence (total ~300bp) into our 3UTR microRNA
expression lentivector, and sequentially produce 0.5 ml shRNA lentivirus.
For CRISPR / CAS9 genomic editing lentiviurs: we will provide (1)
standalone humanized and neuclear penetrating CAS9 expression lentivirus; (2)
design and construct gene specific gRNA lentivector and produce gRNA
lentivirus; (3) synthesize the desired repair DNA element, or the knockdown-in
marker cassette when requested;
Lentiviral service request form, Page 1 of 7
www.gentarget.com ; GenTarget Inc Copyrights, 2015
7930 Arjons Drive, Suite B
San Diego, CA 92126
Phone: 858-6788683
Fax:
800-3804198
Email: orders@gentarget.com
Please provide answers the following questions, so we can give you a specific
price quote. You only answer the section that related to your service request.
(A) For shRNA lentivirus (see lentivector's core map scheme below):
1. Can you provide the shRNA target sequence (usually 19-25bp) or do you
need GenTarget to design the shRNAs for you (if we design it, please provide
us the gene name, better the gene accession number)?
Type in:
If to provide your shRNA sequences, please list your shRNA sequences below:
Type in:
2. Please, select the promoter for express the shRNA sequence:
 Human H1 optional inducible promoter,
 Human U6 constitutive promoter,
3. Please, select shRNA lentivector’s fluorescent reference marker (select only
one marker):
 GFP-Puromycin Marker,
 GFP -Blasticidin Marker,
 RFP-Blasticidin Marker,
 RFP -Puromycin Marker,
 Puromycin Marker,
 Blasticidin Marker,
4. Gentarget delivery 0.5ml of each lentivirus as default, if you need more virus,
please specify the volume (in ml) below.
Type in:
Lentiviral service request form, Page 2 of 7
www.gentarget.com ; GenTarget Inc Copyrights, 2015
7930 Arjons Drive, Suite B
San Diego, CA 92126
Phone: 858-6788683
Fax:
800-3804198
Email: orders@gentarget.com
(B) For over expression lentivirus (see the lentivector map schemes below):
1. Gene name (with specie), or accession number :
Type in:
2. Can you provide the gene template (need to provide 5 ug of template
plasmid DNA, in any plasmid backbone), or do you need GenTarget to either
synthesize it or obtain it from cDNA collection?
Type in:
3. Please select the promoter (select only one promoter) :
suCMV,
EF1a,
Inducible suCMV,
CAG promoter,
Lentiviral service request form, Page 3 of 7
www.gentarget.com ; GenTarget Inc Copyrights, 2015
7930 Arjons Drive, Suite B
San Diego, CA 92126
Phone: 858-6788683
Fax:
800-3804198
Email: orders@gentarget.com
4. Please select tag :
No, do not include His-tag,
Put 6His-tag at N-terminal of the gene,
Put 6His-tag at C-terminal of the gene,
Other tags, please provide the sequence and position
Type in:
5. Please select marker (only select one marker):
Use Blasticidin marker,
Use Neomycin marker,
Use Puromycin marker,
Use GFP-Blasticidin marker,
Use GFP-Blasticidin marker,
Use RFP-Puromycin marker,
Use RFP-Puromycin marker,
Use firefly luciferase marker ,
Make the bicistronic expression target and a fluorescent marker (via 2A
element)
,
Any specific requests:
Type in:
6. Is 0.5ml of virus enough, or do you need more (if more, please precise)?
Type in:
(C) For promoter signaling lentivirus (see the lentivector map schemes below):
Your choice promoter name and sequence:
Type in:
Please select marker (only select one marker):
Use Blasticidin marker,
Use Neomycin marker,
Use Puromycin marker,
Use GFP marker,
Use RFP marker,
Lentiviral service request form, Page 4 of 7
www.gentarget.com ; GenTarget Inc Copyrights, 2015
7930 Arjons Drive, Suite B
San Diego, CA 92126
Phone: 858-6788683
Fax:
800-3804198
Email: orders@gentarget.com
(D) For anti-microRNA lentivirus (see the lentivector map schemes below):
1. microRNA ID, name (with specie), or matured miRNA sequence :
Type in:
2. Please, select shRNA lentivector’s marker (select only one marker):
 GFP-Puromycin Marker,
 GFP-Blasticidin Marker,
 RFP-Blasticidin Marker,
 RFP-Puromycin Marker,
 Puromycin Marker,
 Blasticidin Marker,
3. Please, select the promoter for express the anti-miRNA sequence:
 Human H1 optional inducible promoter,
 Human U6 constitutive promoter,
3. Is 0.5ml of virus enough, or do you need more (if more virus, please
precise)?
Type in:
(E) For precursor microRNA expression lentivirus: (see the lentivector map
schemes below):
Lentiviral service request form, Page 5 of 7
www.gentarget.com ; GenTarget Inc Copyrights, 2015
7930 Arjons Drive, Suite B
San Diego, CA 92126
Phone: 858-6788683
Fax:
800-3804198
Email: orders@gentarget.com
1. microRNA ID, name (with specie):
Type in:
Human and mouse microRNA precursors and its native context
sequences (upstream and downstream flanking genomic sequences)
were PCR amplified and cloned into a pLenti-TetCMV(miRNA)Rsv(Puro) lentivector.
3. Is 0.5ml of virus enough, or do you need more (if more virus, please
precise)?
Type in:
(F) CRISPR / Cas9 gene editing lentivirus (see the lentivector map schemes
below):

CAS9 expression lentivectors:

gRNA lentivectors:
Gentarget provide premade CAS9 expression lentivirus with different antibiotic /
fluorescent markers, and design and construct gene specific guild RNA lentivectors,
Lentiviral service request form, Page 6 of 7
www.gentarget.com ; GenTarget Inc Copyrights, 2015
7930 Arjons Drive, Suite B
San Diego, CA 92126
Phone: 858-6788683
Fax:
800-3804198
Email: orders@gentarget.com
then produce ready-to-use targeting gRNA lentivirus, and optionally synthesize the
double stranded loci specific knock-in cassette for gene editing.
Describe service request below:
Lentiviral service request form, Page 7 of 7
www.gentarget.com ; GenTarget Inc Copyrights, 2015